REDEFEND
REDEFEND
REDEFEND
INDICATOR
• Cadeliña,Rosejane
• Sasing, Klenth
Ms. ElfinaCandelario
RESREACH INSTRUCTOR
INDICATOR
____________________________
___________________________
Requirements for
____________________________
By
• Cadeliña,Rosejane
• Sasing, Klenth
AKNOWLEDGEMENT
Foremost, we would like to give our deepest and warmest gratitude to our research
professor, Engr. Elfina Z. Cadenlario for his patience, motivation, immense knowledge and
continuos support in the making of our research paper. We wouldn't have done all of this without
you, madam.
We would also like to give our thanks to Cebu Doctors University for allowing us to
Nobody has been more important to us in the pursuit of this project than our families. We
would like to thank our parents, whose love and guidance are always with us in whatever we
Last but not the least, we would like to express our gratefulness and praise to our God.
Thank you for keeping us safe and away from danger, for giving us good health and letting us
wake up and be productive everyday. We would not be here without your guidance and for that,
ACKNOWLEDGMENT 3
LIST OF FIGURES
ABSTRACT
CHAPTER
INTRODUCTION
Rationale 6-7
THE PROBLEM
Research Design 16
Research Environment 17
Research Procedures
Gathering of Data 17
INTRODUCTION
Rationale
different conditions. One angle considered is the shade reaction which can be resolved by means
of the utilization of markers. A pointer is a substance that changes shading, contingent upon
cautioning signs are colors that are themselves helpless acids and powerless bases. One marker
that almost every individual mates with acids and bases is the litmus, which can possibly change
Chemistry because of the reality its shading interchange is extremely self-evident, making it
helpful to utilize. There are numerous different indications that trade shade at various pH levels,
as are valuable for outstanding purposes. Another for the most part utilized pointer, the pH paper,
comprises of a blend of various side effects that substitute hues at exceptional pH levels.
Corrosive - Base markers (otherwise called pH pointers) are substances which change shading
with pH. They are typically powerless acids or bases, which when disintegrated in water separate
somewhat and structure particles. In spite of the directly accessible instrumental systems for the
synthetic examination of different examples, the customary strategies for investigation are as yet
proper in many applications. Pointers are colors or colors that can be segregated from an
assortment of sources, including plants, parasites, and green growth. For all intents and purposes
any bloom that is red, blue, or purple in shading contains a class of natural shades known as
anthocyanin that can change shading with pH. Some normally hued substances change hues
when the sharpness or alkalinity of their condition changes, for instance, grape juice, darker tea,
and some bloom shades. These substances are called corrosive/base markers. Markers change
shading at a specific phase of concoction response various usually utilized pointers in the labs
are methyl red, methyl orange, phenolphthalein, phenol red, methyl yellow, pentamethoxy red,
bromophenol blue, thymol blue, etc.. Most sort markers are accessible for various kinds of
titrimetric investigations.
For corrosive base titrations, natural colors, which are either powerless acids or bases,
serve superbly as markers. Corrosive base markers are substances that are uncovered through the
trademark shading which changes the level of causticity or alkalinity of arrangements. The
decision of a pointer for a specific titration relies upon the normal for the balance bend. In
corrosive base titration, a pointer is utilized to decide the end purpose of the titration at which the
corrosive and base are in the definite extents important to frame salt and water as it were. As of
late, there has been an expanding enthusiasm for the look for elective wellsprings of pointers
from normal wellsprings of plant starting point. These choices from plant beginning are most
likely less expensive, promptly accessible, simple to extricate, less poisonous to clients, and
naturally neighborly. For comfort, numerous research centers pick to utilize pH meters or pH
pens. Many plant shades found in nature can be utilized viably as corrosive base pointers. The
colors in blueberries and red cabbages have a place with a group of atoms called anthocyanins.
This group of particles is in charge of the shading found in radishes, eggplants, and various
blossoms. The anthocyanins are water-solvent and have hues that are reliant upon the pH of the
responding arrangement. In this examination, the analyst tried the practicality of mayana and
camote tops concentrate as a corrosive base marker. Mayana (blumei coleus) contains rosmarinic
a 7.5 ph level which is a totally steady and camote tops, which has a powerless corrosive.
Theoretical Background
In corrosive base titrations, Indicators are utilized to decide the end point (identicalness
point). The marker demonstrates sharp shading change concerning an adjustment in pH. Usually
utilized pointers for acid-base titrations are engineered. Every marker displays an alternate scope
of hues at various pH esteems. They are found to have risky impacts in the human body and
contaminate the earth. They are marginally costly and are inaccessible, so an endeavor is made to
In the course of recent years, a plenty of therapeutic plants has been put to preliminary as
the wellspring of multifunctional green substance operators. The exceedingly shaded shades
acquired from plants are found to show shading changes with variety of pH. Normal colors are
eco-accommodating in nature. Flavonoids are hued shades that can be disengaged from different
pieces of plants like blossoms, foods grown from the ground pH delicate. Flavonoids show
surprising changes in shading with the adjustment in pH because of this property; it offers a
chance to utilize it as a corrosive base titration pointer as opposed to the customary engineered
markers. In this way, it has been conjectured that the bloom concentrate could be used as a
pointer for various kinds of corrosive base titrations. Quisqualisindica L. is a creeper with red
bloom bunches otherwise called 'Rangoon creeper' has a place with the Combretaceae family
local to Asia.
Pentaslanceolata otherwise called 'Egyptian star group' is a types of blossoming plant in
known as 'lutki' is a blooming plant in the family Melastomataceae. Impatiens acaulis ordinarily
known as 'shake resin' that local to Western Ghats of India and Sri Lanka.Rutin and
study uncovers that there is no writing identified with corrosive base pointer property of the
above plant. Thus, the present examination is to explore the corrosive base marker property of
was gathered from Alto-Porvorim, Goa-403521. The blossoms of Pentaslanceolata was gathered
were gathered from Mangeli, Maharashtra-416512. Every one of the synthetic concoctions and
reagents like hydrochloric corrosive (HCl), Sodium hydroxide (NaOH), Acetic Acid
(CH3COOH), Ammonia (NH3) and phenolphthalein, and so forth was secured from Sigma
Aldrich, E-Merck (Germany) and S D Fine Chemicals (India). The crisp blossoms of
cleaned with refined water and cut into little pieces with the assistance of scissors independently.
Bits of each blossom were absorbed 25 ml ethanol for 24 h. The arrangement was sifted
to expel remaining bloom matter and exposed to dissipation to evacuate the dissolvable and
utilized as a marker in AcidBase titrations. The concentrate was saved in a firmly shut
Choi, Inyoung (2017). Another colorimetric pH pointer film was created utilizing agar,
potato starch, and regular colors separated from purple sweet potato, Ipomoea batatas. Both agar
and potato starch are strong networks used to immobilize regular colors, anthocyanins. The
bright unmistakable (UV-vis) range of anthocyanin remove arrangements and agar/potato starch
films with anthocyanins demonstrated shading varieties to various pH esteems (pH 2.0-10.0).
Fourier change infrared (FT-IR) and UV-vis area spectra demonstrated similarity between agar,
starch, and anthocyanin removes. Shading varieties of pH marker films were estimated by a
colorimeter after drenching in various pH cushions. An application test was led for potential use
as a meat waste sensor. The pH marker films indicated pH changes and waste purpose of pork
tests, changing from red to green. Along these lines, the created pH marker movies could be
In acid-base titrations, Indicators are used to determine the end point (equivalence point).
The indicator shows sharp color change with respect to a change in pH. Commonly used
indicators for acid base titrations are synthetic. Each indicator exhibits a different range of colors
at different pH values. They are found to possess hazardous effects in the human body and
pollute the environment. They are somewhat costly and are inaccessible, so an endeavor is made
to get ready normal marker from plant parts. In the course of recent years, a plenty of restorative
plants has been put to preliminary as the wellspring of multifunctional green substance operators.
The profoundly hued shades acquired from plants are found to show shading changes with
influence customers and can in like manner cause biological pollution. Thus there has been a
growing energy for filtering for elective wellsprings of markers from trademark beginnings.
These alternatives would be more affordable, logically available, simple to evacuate, less deadly
to customers and earth very much arranged. Volumetric examination is one of the key
destructive correspondence with strong or frail acids also, bases in crude materials, intermediates
and completed items for quality confirmation purposes. This is cultivated by means of the
utilization of suitable frail natural colors or acids pH pointers. Most pH pointers are either
powerless natural acids or bases colors which acknowledge or give electrons. The adjustment in
shading at a negligible range is ascribed to their acridity or basicity properties. In spite of the fact
that there are mechanized titration device that decide the proportionate focuses between
responding species, markers are as yet required for educating and research labs for basic titration.
Common markers have been separated from Hibiscus (red species), Bougainvillea and rose
blossoms. A few creators have investigated the viability of common markers in corrosive base
alba linn natural product remove marker and Ixoracoccinea, Daturastramonium, Sun blossom
palmate) bloom petal extricates. The common pointer sources researched in these papers have
Wadkar K An et al. (2008) expressed that Waakye leaves are gotten from Guinea corn
which is developed for the most part in African nations and different pieces of the world. The
plant is recorded as the fourth significant world oat crop base on its creation and is additionally
considered as staple nourishment in semi-parched tropics. The leaves are considered as great
color hotspots for kicking the bucket caps, totes and wallets in Ghana (Azumdo 2006).Besides
the above critical part of the leaves, they have been accounted for to contain basic unsaturated
fats and vital bioactive mixes, for example, lycopene, beta-carotene, phenolics, chlorophyll an
and chlorophyll b, dense tannins and flavonoids On a past report did by Azumdo 2006 colors
were separated from the guinea corn leaves and utilized for titration. Be that as it may, the
information was not steady and little is thought about whether the colors could respond well with
a wide range of responding species (solid bases versus solid acids as well as frail acids versus
solid base or the other way around or feeble acids versus powerless bases). Likewise no proof is
accessible with respect to the pH scopes of the pointer, its ideal capacity and its conceivable
Deshpande An et al. (2010) assessed The bloom concentrate of Ixoracoccinea for its
utilization as a corrosive base marker in different corrosive base titrations, and revealed
consequences of this screening contrasted and the outcomes acquired by standard pointers
methyl orange, blended pointer [methyl orange: bromocresol green for solid corrosive v/s solid
base (HCl and NaOH), Strong corrosive v/s frail base (HCl and NH4OH), feeble corrosive v/s
solid base (Oxalic corrosive and NaOH), and powerless corrosive v/s powerless base (Oxalic
corrosive and NH4OH) titrations green to pink and blue to pink shading separately at end point
Sharma P et al. (2013) and Jain P et al. (2012) analyzed Dahlia pinnata for its corrosive
base marker movement against phenolphthalein, methyl orange and phenol red as standard
pointer. The macerated hydro alcoholic 40:60 Aq. what's more, methanolic petals concentrate of
blooms utilized and it demonstrates lackluster to yellow for solid corrosive v/s solid base, feeble
corrosive v/s solid base while it swings yellow to boring for powerless base with solid corrosive
and frail corrosive and frail base titration inside pH run 3-5 against methyl orange (ethanolic
remove). Methanolic and aq. Concentrate has orange to wine red, pink to red yellow and orange
to yellow shading at end point. This investigation used to be led to almost certainly decide
whether the camote leaf extricate, a showed pH pointer, could be assimilated and dried on paper
without the concentrate dropping its capacity to substitute in shading upon exposure to various
degrees of pH. It proposes an expanded method for the utilization of and putting away the
camote extricate. The gathering, in the wake of demonstrating the possibility of camote pH
papers, defined elite research facility conventions and classroom activities in regards to acids and
bases that would help educators in presenting that exercise without losing expensive industrially
reachable pH papers.
Camote leaf separate was once acquired through heating up the camote tops. This
concentrate was once consumed by channel paper. Subsequent to drying the channel papers,
experimenting with was once led. The camote pH papers were diminish into strips and
submerged into explicit acids and bases. The outcomes for shading adjustment have been certain.
The kamote pH papers swings to neon pink when presented to acids and it swings to a splendid
unpracticed when submerged in essential arrangements. The power of the shading is lessened as
sharpness diminishes as it likewise does with bases. Besides, the handiness of the paper is never
again traded off with time as opposed to the snappy time span of usability of fluid concentrates.
synthetic substances and it even has affectability to exhaust. It have to never again be ignored
that other plant concentrates could be utilized as a base concentrate for this pH marker. This find
out about has, in any case, been fit to demonstrate the limit of the concentrate to hold its
corrosive base marker. This examination looks at how it is as a pH pointer. The two shades of
camote leaves had been utilized, to be specific, the green and pink assortments. Regardless of
whether they have extensive contrasts in the sign dimension was also researched. Shading
with perceived pH ranges were treated with tests of both the unpracticed and red leaf
concentrates, and results were contrasted and estimations taken with other monetarily utilized
markers like pH meters and pH papers. Respondents were asked for on the off chance that they
saw shading changes because of pH in the managed tests, regardless of whether the tints were
distinguishable, and whether they favored red or green. It was once discovered that the two sorts
created worthwhile outcomes, with recognizable shading changes for every single pH level.
Between the two, in any case, progressively individuals wanted the purple assortment, as shade
adjustments had been additional pronounced. The oversee group is the team that stays the
equivalent and is currently not changed at all. One gathering, known as the control gathering, is
revealed to the majority of the circumstances of the test anyway does not get something is being
analyzed or changed in the test. At the equivalent time, the test group gets the majority of the
circumstances of the examination PLUS the one variable being analyzed by method for the trial.
Smelling salts arrangement, also viewed as ammonium hydroxide, alkali water, ammonical
alcohol, alkali alcohol, water alkali, fluid smelling salts, or genuinely smelling salts, is an answer
of smelling salts in water. Kalamansi juice is a corrosive. It has gentle harsh taste. Harsh taste is
a normal for an acidic property. Ammonium hydroxide, NH4OH, which is a frail base.
(Buenaventura, 2004, p. 20) Today engineered markers are the inclination of corrosive base
titrations. In any case, because of ecological contamination, accessibility and cost, the look for
characteristic mixes as a corrosive base pointer was once begun. The present employment
features the take advantage of the fermented methanolic concentrate of the blossoms of couple of
therapeutic vegetation as a corrosive base marker in corrosive base titrations. This common
outcomes had been gotten when it was once looked at against in vogue engineered pointers.
Titration demonstrates sharp shading trade at the proportionality point. The identicalness factors
got by method for the blossoms remove concur with the comparability factors acquired by
utilizing far reaching pointers. These home grown cautioning signs are seen to be a valuable,
corrosive base titration which is separated from guinea corn leaves prevalently known as
"waakye leaves" in Ghana. Four sorts of corrosive base titration were contemplated: solid
corrosive versus (v/s) solid base, solid corrosive versus frail base, powerless corrosive versus
solid base, and feeble corrosive versus powerless base. The pointer tinge change, pH run and the
normal titre esteems had been resolved for each sort of corrosive base titration.
Conceptual Framework
Mayana and
Acid Base
Camote Tops Boiled
Indicator
leaves
The main aim of this study is to have an eco friendly, natural acid base alternative acid base
indicator for the benefit of the school, researchers and for the students to have an inexpensive
comparative study of mayana and camotetops as acid base indicator in order to make an
THE PROBLEM
Our research is based on comparative study of mayana and camote tops as an acid-base
indicator.
Considering acid-base indicator using litmus paper is a high costly acid-base indicator so the
it's safe or not, it may have low acid or not. This study should be done so that people would be
able to have an eco-friendly, natural and safe acid-base indicator. The findings of this study will
Researchers. The success of the study would deliver an experience of achievement and
Students. They will be able to use the mayana and camote tops as an effective and safe
Teachers and Public schools located in remote areas. They can use mayana and
frequently used commercial indicators provided that these extracts that we get is
The scope of this study is to investigate comparative study of mayana and camote tops as
acid base indicator in order to make an alternative natural acid- based indicator— specifically,
variables such as the effectivity of the mayana and camote tops as an indicator, which among the
mayana and camote tops as the most effective acid level indicator, and how helpful to the
This study is limited only to schools who are poor in equipments especially for an indicator
equipment. This study started on November 2018and is expected to be finished on March 2019.
A weakness of this study is that the experiment is only for chosen schools and the findings will
RESEARCH METHODOLOGY
Research Design
The research method or the research design that we will use is comparative and experimental
study (quantitative method). Experimental research is any research conducted with a scientific
approach, where a set of variables are kept constant while the other set of variables are being
measured as the subject of experiment and comparative research can help the us researchers to
ascend from the initial level of exploratory case studies to a more advanced level of general
theoretical models, invariance’s, such as causality or evolution by comparing the two (mayana
and camote tops) which has the effective cid-base indictors as we test some water soluble
products pH level. Since the researchers want to compare which has the effective indictors and
experiment the ph level of plants, fruits and water soluble which is why we use these research
design. The researchers use these studies because we can compare and experiment and we can
test out the pH level of something and to know its effectiveness. We use experimental therefore
Research Environment
The researchers conducted the study to the general public specifically it focuses to
students, in order to help schools that could not afford an acid-base indicator. Acid-base
indicators are substances which change color depending on the level of the acidity with pH, they
are usually weak acids or bases. The researchers decided to use an acid-base indicator to
determine the affectivity of mayana plant and camote tops. The said study will help the target
audience choose the most effective acid-base indicator, the mayana plant or camote tops.
Research Procedures
Preliminary procedures
The researchers began the study by researching and browsing the problems in
today's time. The researchers found out that there are schools that couldn’t afford or
doesn’t have access to acid base indicators. The researchers thought that it would be a big
help if the researches would be able to make an organic acid base indicator since that is
easily found in the areas where the schools are located. With the help of the moderator,
mayana and camote tops as organic acid base indicators. Afterwards, review of related
studies was made, statement of the problem was finalized, null hypothesis was created
and scope and delimitations were stated. Researchers decided to experiment in the
Gathering data:
(plectranthusscutellarioides) and camote tops (ipomoea batata leaf) as acid base indicator. Spata
(2003), states that comparative research may examine differences and similarities between the
variables of the study. The researcher collected data through experimentation. The data shows
which one of the two is more effective as an alternative natural acid- based indicator. The data
also shows if both, mayana (plectranthusscutellarioides) and camote tops (ipomoea batata leaf),
The researcher gathered all the materials needed which is: Mayana leaves,
Camote tops leaves, 2 beakers, ethanol, kalamansi juice, oil, soda, vinegar, HCl, sodium
hydroxide, soapy water ( water mixed with dishwashing soap), 2 bunsen burner, testubes,
2-4 droppers, 1 mortar and pestle, 2 stirring rod, 2 iron stand and wire gauze. The
researchers used 10 leaves in both mayana and camote tops and we crushed or tear them
into small pieces. The researchers had 2 types of solutions for both mayana and camote
tops. The first one is that the researchers boiled the leaves for 15- 30 mins and the other
one is the researchers soaked the leaves in ethanol for 10 mins and crushed the leaves
even further. After collecting the solutions, the researchers prepared 4 test tubes each of
Kalamansi juice, oil, soda, vinegar, HCl, sodium hydroxide, soapy water measuring 10
ml each. The researchers placed 5 drops of the solutions in each samples. The researchers
first dropped the solution extracted from the boiled leaves then followed by the leaves
soaked in alcohol. The researchers observed the color change of each sample and
compared whether the boiled leaves or soaked leaves is more effective and also which
one of the mayana and camote tops solution shows more accurate ph reading.
Indicator Solutions Original Strong Acid Weak Acid Neutral Strong Weak Base
Color Base
Mayana Dark
Violet
Juice
Water Yellow
Hydroxide
Tops Green
Juice
(dark
green)
blue)
Hydroxide blue)
Conclusion
We the researchers, conclude that both boiled Mayana and Camote Tops leaves is more effective
when boiled compared to the leaves with ethanol. Both leaves can determine acidity and can
show it in different ph levels but the Mayana leaves is more Precise and Accurate in determining
the PH level of the acids. So boiled Mayana leaves is better than Boiled Camote tops.
Definition of Terms
family Lamiaceae.
3. Camote tops - (Ipomoea batatas) is a dicotyledonous plant that belongs to the bindweed or
4. Ph level - A measure of acidity or alkalinity of water soluble substances (pH stands for
'potential of Hydrogen').
6. Quantitative - relating to, measuring, or measured by the quantity of something rather than
its quality.
7. Litmus paper - paper stained with litmus which is used to indicate the acidity or alkalinity of
a substance. Blue litmus paper turns red under acid conditions, and red litmus paper turns
10. Phenolphthalein - a colorless crystalline solid (pink in alkaline solution) used as an acid–
12. colorimetric - a device that analyzes color by measuring a given color in terms of a standard
13. Agar - a gelatinous substance obtained from various kinds of red seaweed and used in
purpose.
15. Matrices - (plural form of matrix) fine material used to bind together the coarser particles of
a composite substance.
18. Spoilage - the action of spoiling, especially the deterioration of food and perishable goods.
product.
References:
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Appendix A
Good day!
We the students of grade 12 section F are writing this letter to ask permission from your
good office to let us use the chemistry laboratory for our investigatory project for our subject 3iS
We need to conduct a research on a comparative study of camote tops and mayana leaves as an
acid-base indicator and the laboratory room will be very helpful to us, we would like to use the
our research. We would be using bunsen burner, mortar and pestle, beaker, graduated cylinder
and droppers. We are looking forward for your kind understanding. Thank you and may God
bless you!
Researchers:
___________________ ____________________
PARTICULARS AMOUNT
Materials
oil P 70
Calamnsi Juice P 56
Softdrinks P 34
Vinegar P 11
Mayana P 50
Camote Tops P 70
TOTAL P 261
Appendix C
Topic
Assigned
Thesis
Proposal
Development
Data
Collection
Data
Processing
and Analysis
Oral Defense
Submission of
Research
Final
Requirements
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