Scribd Logo
Upload

Welcome to Scribd!

  • 94 views

    Select The Right Combination To Diagnose Syphilis

    Uploaded by

    Junita
    This document discusses tests for diagnosing syphilis. It explains that during a syphilis infection, two groups of antibodies are detectable: non-treponemal antibodies which react with nonspecific antigens in an RPR VDRL test, and treponemal antibodies which react with specific antigens of Treponema pallidum in a TPHA test. The RPR VDRL test is a qualitative card test to quickly detect syphilis, while the TPHA test detects T. pallidum antibodies using sensitized avian erythrocytes. Using these two complementary tests provides high sensitivity and specificity for diagnosing syphilis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Select The Right Combination To Diagnose Syphilis

    Uploaded by

    Junita
    94 views2 pages
    This document discusses tests for diagnosing syphilis. It explains that during a syphilis infection, two groups of antibodies are detectable: non-treponemal antibodies which react with nonspecific antigens in an RPR VDRL test, and treponemal antibodies which react with specific antigens of Treponema pallidum in a TPHA test. The RPR VDRL test is a qualitative card test to quickly detect syphilis, while the TPHA test detects T. pallidum antibodies using sensitized avian erythrocytes. Using these two complementary tests provides high sensitivity and specificity for diagnosing syphilis.

    Original Description:

    Serologi smk

    Original Title

    rpr_vdrl_2013011

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document discusses tests for diagnosing syphilis. It explains that during a syphilis infection, two groups of antibodies are detectable: non-treponemal antibodies which react with nonspecific antigens in an RPR VDRL test, and treponemal antibodies which react with specific antigens of Treponema pallidum in a TPHA test. The RPR VDRL test is a qualitative card test to quickly detect syphilis, while the TPHA test detects T. pallidum antibodies using sensitized avian erythrocytes. Using these two complementary tests provides high sensitivity and specificity for diagnosing syphilis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    94 views2 pages

    Select The Right Combination To Diagnose Syphilis

    Uploaded by

    Junita
    This document discusses tests for diagnosing syphilis. It explains that during a syphilis infection, two groups of antibodies are detectable: non-treponemal antibodies which react with nonspecific antigens in an RPR VDRL test, and treponemal antibodies which react with specific antigens of Treponema pallidum in a TPHA test. The RPR VDRL test is a qualitative card test to quickly detect syphilis, while the TPHA test detects T. pallidum antibodies using sensitized avian erythrocytes. Using these two complementary tests provides high sensitivity and specificity for diagnosing syphilis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 2

    RPR VDRL CARBON / TPHA

    S e r o lo g y r a p i d t e s t s

    Select the right combination to


    diagnose Syphilis

    2 complementary agglutination tests for the


    detection of treponemal and non-treponemal
    antibodies in serum

    All-in-one kits (controls included)


    Qualitative or semi quantitative approaches available
    Cost effective, routine laboratory test
    High sensitivity and specificity
    Fast results for clinicians

    Treponema pallidum
    Principle
    During Syphilitic infection, 2 groups of antibodies are detectable: nontreponemal antibodies which react with
    nonspecific antigens (RPR VDRL test), and treponemal antibodies which react with the specific antigens to
    T. pallidum (TPHA test).

    RPR VDRL test is a serologic cardiolopidic and nontreponemic test for the quick detection of Syphilis. The reagent
    consists of a suspension of cardiolipin/lecithin/cholesterol and carbon particles in order to improve the visual
    reading. Cardiolipidic antigens react with the antibodies (reagin) present in the sample and form macroscopically
    visible black dumps.

    TPHA test detects human T. pallidum antibodies by an haemagglutination method. Avian erythrocytes are
    sensitized with T. pallidum fragments. In the presence of specific anti-T. pallidum antibodies, sensitized erythrocytes
    (Test Cells) agglutinate resulting in characteristic clouding at the bottom of the wells of the microtitre plate. In the
    absence of antibodies, they sediment and form a very dense ring or compact button in the bottom of the well.
    Unspecific reactions are detected using Control Cells which are unsensitized erythrocytes.

    Methodology
    TPHA (QUALITATIVE PROCEDURE) : RPR VDRL (QUALITATIVE PROCEDURE) :
    1 Mix 190 µL diluent 1 Dispense 1 drop of 2 Dispense
    + 10 µL serum serum / plasma 1 free-falling
    3 Transfer 25 µL or control(s) drop of the antigen
    or control(s)
    of mixture suspension

    2 Remove 150 µL

    4 Add 75 µL of: Test Cells / Control Cells

    Tap plate gently to mix the


    contents thoroughly Rotate 8 min
    Cover and let stand 45 to at 100 r.p.m
    60 min at Room Temperature

    Results are expressed as an agglutination intensity (- to 4+). Examine immediately the presence or absence of
    visible agglutination.

    Negative results 1 : Non reactive

    2 : Weakly reactive
    4+ 3+ 2+ 1+ +/-
    3 : Strongly reactive
    Positive results

    All positive results (+/- to 4) must be tested with the For the semi-quantitative procedure, please refer to the
    Part nr: EMR TPHA RPR VDRL-490-2013/00EN

    semi-quantitative procedure (see technical insert). technical insert.

    Product range
    REF. NAME QTY SENSITIVITY SPECIFICITY

    RPRL-0100 RPR VDRL CARBON 100 tests >99% >99%


    TPHA-0100 TPHA 100 tests 98.5% 99.6%
    TPHA-0004 MICROPLATES 5 units
    Product not available in the US.
    Please contact your sales representative for product availability in your country

    Headquarters
    ELITechGroup
    T: +33 1 41 45 07 13 Serbia T: +381 11 2467119
    Belgium T: +32 9 2820 531 Switzerland T: +41 26 663 86 60
    Brazil T: +55 27 3025 1415 The Netherlands T: +31 313 430574
    France T: +33 4 94 88 55 00 UK T: +44 1442 869320 S O L U T I O N S
    Italy T: +39 02 48 40 35 42 United States T: +1 609 216 736 tailored to your needs
    Middle East & Africa T: +971 4 375 2744 855-ELITECH (855-354-8324) info@elitechgroup.com • www.elitechgroup.com

    You might also like