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    Separation of DNA

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    Patricio Najeal
    The document summarizes an experiment to isolate DNA from a saliva sample. Saltwater was swirled in a person's mouth to collect cells, then mixed with dish soap and alcohol. This caused whitish, strand-like DNA to emerge suspended in the solution, with gas bubbles appearing around it. The visual appearance confirmed that DNA had been successfully isolated from the saliva in an aqueous solution.

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    Separation of DNA

    Uploaded by

    Patricio Najeal
    36 views2 pages
    The document summarizes an experiment to isolate DNA from a saliva sample. Saltwater was swirled in a person's mouth to collect cells, then mixed with dish soap and alcohol. This caused whitish, strand-like DNA to emerge suspended in the solution, with gas bubbles appearing around it. The visual appearance confirmed that DNA had been successfully isolated from the saliva in an aqueous solution.

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    lab report

    Original Title

    separation of DNA

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    The document summarizes an experiment to isolate DNA from a saliva sample. Saltwater was swirled in a person's mouth to collect cells, then mixed with dish soap and alcohol. This caused whitish, strand-like DNA to emerge suspended in the solution, with gas bubbles appearing around it. The visual appearance confirmed that DNA had been successfully isolated from the saliva in an aqueous solution.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    36 views2 pages

    Separation of DNA

    Uploaded by

    Patricio Najeal
    The document summarizes an experiment to isolate DNA from a saliva sample. Saltwater was swirled in a person's mouth to collect cells, then mixed with dish soap and alcohol. This caused whitish, strand-like DNA to emerge suspended in the solution, with gas bubbles appearing around it. The visual appearance confirmed that DNA had been successfully isolated from the saliva in an aqueous solution.

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    Isolating deoxyribonucleic acid from aqueous

    solution
    Patricio Jose H. Najeal, Kurt Matthew Amodia

    The objective of the experiment was to isolate DNA from saliva sample and to observe its
    visual appearance. The sample was extracted by swirling salt in water solution in a human
    mouth. Part of the sample was transferred to a test tube. The sample was mixed with liquid
    dishwashing solution and alcohol was allowed to suspend from the solution. DNA was
    observed to be whitish and with strand-like shape.

    Keywords: DNA, saliva, isolate, extract


    I. Introduction III. Results and Discussions
    Deoxyribonucleic acid (DNA) is a The sample was observed to have
    molecule composed of two chains that coil white strand-like masses with bubbles of gas
    around each other in a double helix structure. surrounding them. These white strand-like
    DNA carries genetic information for the masses were taken to be DNA.
    growth, development, and reproduction of all
    IV. Conclusion
    known organisms.
    DNA was successfully isolated in
    The DNA in saliva originates from
    aqueous solution. DNA was observed to have
    cells that are shed from the inner linings of
    whitish color and strand-like shape. Gas
    the mouth and from white blood cells. 1
    bubbles also started to appear around the
    The objective of the experiment is to DNA.
    isolate DNA from saliva, and to observe and
    V. References
    describe the visual appearance of DNA.
    1) Salimetrics. (Retrieved February 28,
    II. Methodology
    2019). Collecting and Handling Saliva for
    Rubbing alcohol was placed in a DNA Analysis. Retrieved from
    freezer for an hour in preparation for the https://www.salimetrics.com/collecting-and-
    experiment. Four identical containers, and a handling-saliva-for-dna-analysis
    test tube were also prepared. In one container,
    VI. Acknowledgements
    30 mL of tap water was mixed with 5 mL of
    iodized salt. In a separate container, 15 mL of We would like to thank Miss Sherry
    tap water and 5 mL of liquid dishwashing Ramayla for giving us the opportunity to
    soap were mixed. 2 mL of the saltwater conduct the experiment. We would also like
    solution was taken from the first container to thank the Philippine Science High School
    and placed on a separate container. The – Central Visayas Campus for their
    newly isolated saltwater solution was swirled unwavering support for us.
    in human mouth for 30 seconds, after which
    the human spit the saltwater solution in the VII. Appendix
    fourth container, which samples DNA from
    the human.
    1 mL of the sample was transferred to
    the test tube for ease of observation. 1 mL of
    the soap water solution from the second
    container was added to the test tube and was
    allowed to mix with the sample for 1 minute.
    After the contents were mixed, 15 mL of cold
    rubbing alcohol were transferred to the test
    tube. The alcohol was transferred slowly to
    prevent mixing the alcohol with the sample. Figure 1: Isolated DNA from aqueous solution
    The sample was observed for 1 minute.

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