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Plant Propagation Laboratory Introduction and Exercise: Controlled Pollination

1. Controlled pollination is used in plant breeding to produce superior seedlings from selected parent plants. This involves bringing chosen male and female parents into flower at the same time, collecting pollen from the male, emasculating the female, and applying the pollen. 2. The basic steps of controlled pollination include selecting parents, bringing them into flower, collecting pollen, emasculating and pollinating the female, protecting the flower, recording the cross, and allowing the seeds to mature. 3. Students will practice controlled hand pollination of petunias in the lab, making crosses according to the provided data sheet to collect and harvest seeds within a week.

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236 views2 pages

Plant Propagation Laboratory Introduction and Exercise: Controlled Pollination

1. Controlled pollination is used in plant breeding to produce superior seedlings from selected parent plants. This involves bringing chosen male and female parents into flower at the same time, collecting pollen from the male, emasculating the female, and applying the pollen. 2. The basic steps of controlled pollination include selecting parents, bringing them into flower, collecting pollen, emasculating and pollinating the female, protecting the flower, recording the cross, and allowing the seeds to mature. 3. Students will practice controlled hand pollination of petunias in the lab, making crosses according to the provided data sheet to collect and harvest seeds within a week.

Uploaded by

Sidharth Behera
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Plant Propagation Laboratory Introduction and Exercise

Module 2

Controlled Pollination
Most plant improvement comes about through seeds produced via the normal sexual cycle.
This occurs through the selection of superior individual seedlings from the seedling progeny
resulting from cross-pollination between chosen parents. These superior seedlings are then
called "selections", and may be propagated asexually or sexually, depending on the plant
species involved.

The basic steps in controlled pollination are as follows:

1. Selection of your parent plants.

2. Bringing chosen parents into flower simultaneously, if possible.

3. Collection of pollen from the male parent. Short or long term storage of the collected pollen
may be necessary.

4. Emasculation (removal of male parts) and pollination of the selected female parent.
Emasculation is not necessary if the flowers are dioecious or male sterile, or if the flower is
to be self-pollinated.

5. Protection of the pollinated flower - not usually necessary if the pollination is made indoors.
Records are made of the pollination. The effectiveness of the pollination is usually judged
from fruit and seed set percentages.

6. Growing of ripening fruit resulting from the pollination to maturity.

7. Extraction of seeds from fruit; cleaning, drying and storage of seeds. The method of seed
extraction is variable, depending on the fruit type.

8. Seed germination. A pretreatment to aid in seed ripening or to overcome dormancy prior to


germination is frequently necessary.

9. Seedling growth and transplanting until field size is attained.

10. Transplanting to the final evaluation site and growth to maturity.

11. Field selection among the produced hybrids.

12. Propagation of the selected seedlings.

This sequence represents the highlights of one breeding generation.

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Plant Propagation Laboratory Introduction and Exercise
Module 2

Controlled hand pollination practice

You will have the opportunity to pollinate petunias. The procedure will be demonstrated
for you by the laboratory instructor. Record the crosses and collect the seed as they
mature. Complete all of your crosses within a week of the assigned lab so you may
harvest your seed before your final exam.

Controlled Pollination Data Sheet

Cross Male Female Date of Number of


number Parent Parent Pollination Seed produced

1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

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