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Name: YunantoFARMASIB

Exercise 8: Chemical and Physical Processes of Digestion: Activity 1: Assessing Starch Digestion by Salivary Amylase Lab
Report

Pre-lab Quiz Results


You scored 100% by answering 6 out of 6 questions correctly.

1. The substrate for amylase is


You correctly answered: e. starch and carbohydrate.

2. Which of the following is true of enzymes?


You correctly answered: c. Their activity can be affected by temperature and pH.

3. The reagent IKI tests for the presence of


You correctly answered: a. starch.

4. Which of the following is not true of controls?


You correctly answered: d. A negative result with a positive control is required to validate the test

5. Which of the following is an end product of starch digestion?


You correctly answered: e. maltose and glucose

6. Hydrolases are enzymes that break down large molecules into smaller subunits through the addition of _____________.
You correctly answered: b. water

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Experiment Results
You have not completed the Experiment.

Experiment Data:

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. Tubes 3, 7, and 8 reveal that


You correctly answered: d. amylase activity was highest at pH 7.

2. This activity includes a number of negative controls. Which tube indicates that the amylase solution was not
contaminated with maltose?
You correctly answered: b. tube 4

3. Which tubes indicate that the deionized water did not contain contaminating starch or maltose?
You correctly answered: c. tubes 4, 5, and 6

4. Explain where and why salivary amylase would be most active.


You correctly answered: d. Salivary amylase would be most active in the mouth because pH 7 is where its peak activity is.

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Review Sheet Results
1. List the substrate and the subunit product of amylase.
You did not answer this question.

2. What effect did boiling have on enzyme activity? Why? How well did the results this compare with your prediction?
You did not answer this question.

3. At what pH was the amylase activity the most active? Describe the significance of this result.
You did not answer this question.

4. Briefly describe the need for controls and give an example used in this activity.
You did not answer this question.

5. Describe the significance of using a 37°C incubation temperature to test salivary amylase activity.
You did not answer this question.

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