SPECIFICATION SHEET

Real-Time PCR Systems

Applied Biosystems 7500 Fast and

7500 Real-Time PCR Systems
Real Fast. Real Versatile. Real Performance.

Software Highlights The Applied Biosystems Advantage Applied Biosystems® 7500 Fast and 7500
Built on over ten years of real-time Real-Time PCR Systems
• High Resolution Melting (HRM)
expertise, the Applied Biosystems® 7500 • Flexible five-color detection systems are
Software available on the 7500
Fast and 7500 Real-Time PCR Systems easily calibrated for your choice of dyes
Fast System are versatile platforms for the detec- without requiring the addition
• Powerful New Gene Expression tion and quantification of nucleic acids in of new filter sets
Study Package standard 96-well formats. Our integrated
• Plate Setup Wizard walks you effort-
• Customizable 21 CFR Part 11 module real-time PCR solutions combine innova-
lessly through experimental design
• Export to JPEG and PowerPoint® tive thermal cycling systems, powerful
• Configurable graphs and plots software, optimized reagents, your choice • Advanced optical multicomponenting
of off-the-shelf or custom assays, and su- algorithm minimizes spectral cross
perior support for a variety of applications. talk—superior for multiplexing
• Variable excitation capacity allows
greater sensitivity for the greatest range
of dyes including FAM /SYBR® Green,
TM

VIC®/JOE , NEDTM/TAMRA /Cy3®, ROX /

TM TM TM

Texas Red®, and Cy5®

Applied Biosystems® 7500 Fast
Real-Time PCR System
Results in 30 Minutes
The Applied Biosystems® 7500 Fast
Real-Time PCR System offers maximum
Applied Biosystems 7500 Fast Real Time PCR System performance in the minimum time for
labs running a variety of applications,
Applied Biosystems provides pre-formulated, ready-to-use, quality tested 5’ nuclease TaqMan
®

including High Resolution Melting (HRM)

assays for use with 7500 Fast and 7500 Systems.
analysis. Fully optimized for Fast cycling,
Assay Details Applications
the 7500 Fast System delivers high-quali-
Assay Formats Gene Expression SNP Genotyping ty results in as little as 30 minutes.
TaqMan Genomic Assays
®

www.allgenes.com www.allsnps.com The specially designed Peltier-based 7500

Custom TaqMan Genomic Assays
®

Fast block ensures thermal uniformity at

Genome Availability (non-custom) Number of Assays top speeds. Faster ramp rates and novel
Human 196,370 >4,500,000 well design enable rapid results without
Mouse 174,469 10,000 compromising extension times or assay
quality.
Rat 150,001 N/A
D. Melanogaster (Drosophila) 40,520 N/A The Applied Biosystems® 7500 Fast System
A. Thaliana (Arabidopsis) 97,232 N/A is the original Fast solution including vali-
Rhesus Macaque 11,788 N/A
dated Fast reagents and over 785,000 avail-
able TaqMan® Gene Expression Assays.
Canine 22,616 N/A
7500 Software v2.0 More Powerful Gene Expression HRM Software
The 7500 software v2.0 for the 7500 Fast Study Package Use the Applied Biosystems® High
and 7500 Real-time PCR Systems runs on • Import an unlimited number of Resolution Melting (HRM) Software
the Windows® XP and Microsoft Vista OS Comparative Ct (Relative Quantitation) to perform more sophisticated melting
operating systems. The 7500 Software v2.0 plates to one study analysis with an easy to follow workflow and
can be used for instrument control, data • View data by biological replicate group minimal subjective data analysis steps.
collection, and advanced data analysis. or technical replicate group • Shortens analysis time by autocalling
Powerful and user-friendly, 7500 Soft- • Normalize to multiple endogenous genotypes and automatically omitting
ware v2.0 includes: controls the no template controls
• Design Wizard to walk you through
• Enter known efficiencies to adjust RQ
• Minimizes subjective analysis by
experimental design automatically grouping unknown
values for each target
• Advanced setup option offers variant clusters
flexibility for more complex
• View amplification plot, multicomponent
plot and QC summary within the study to
• Allows easy data review with
applications, such as multiplexing customizable multi-plot views,
easily identify and eliminate outliers
• Quick-start setup option allows you expandable windows and one-click
to begin a run immediately and enter
• Preview the effect of modified analysis color assignment to highlight curves
plate information later settings before permanently applying of interest
them to results
• Pipetting protocols and recipes help • Facilitates data presentation with the
you set up experiments quickly option to export data and graphs
Expanded Melt Curve Options directly to PowerPoint or as JPEG files
• Troubleshooting flags to help you
diagnose and resolve problematic
• Option to use either a continuous melt • No temperature shift required –
experiments for standard post PCR melt curves, or distinguish between homozygous
step and hold melt curve for melting mutants and wild type more easily
• Email notification when a run is experiments that require more
complete resolution and accuracy
• One-click graphical export to • More flexible melt curve protocols allow
PowerPoint and JPEG you to customize the ramp rate, tem-
perature span and data acquisition
• Identify the Tm of up to three melt
curve products per well
• Melt curve data can be imported into
the Applied Biosystems® High
Resolution Melting (HRM) Software
for in depth analysis
The Applied Biosystems ® High Resolution
Melting (HRM) Software is the easiest to
use melting analysis software enabling
real-time PCR melt curve assays to be used
more accurately for mutation scanning and
genotyping.
The 7500 Software v2.0 simplifies plate setup
with setup wizards, grouping of biological
replicate groups, and the option to import
previously used genes and samples from
libraries.

Reagents and Disposables Complete Validation Solutions

A complete line of reagents including • IQ/OQ—A certified Applied Biosystems • 21 CFR Part 11 compliance—The SDS 21
TaqMan® Fast Universal PCR Master Mix, Service Engineer will assist you with CFR Part 11 Module is the most flexible
TaqMan® Universal PCR Master Mix, your Installation Qualification and module available to assist with 21 CFR
Power SYBR® Green PCR Master Mix, Operational Qualification (IQ/OQ) or Part 11 compliance using real-time PCR
and disposables including 96-well plates, Instrument Performance Verification systems. Customize the 21 CFR Part 11
is available for use with the 7500 Fast and (IPV) process as part of your overall support tools to meet your compliance
7500 Real-Time PCR Systems. system validation needs
Performance Specifications
Once installed, an Applied Biosystems Demonstrated Performance

Field Service Engineer will ensure your

Instrument 7500 Fast System 7500 System
7500 Fast or 7500 System is performing
to specifications. Using the RNase P Performance
Instrument Verification Plate, we will verify
Dynamic Range 9 logs of linear dynamic range
that:
Sensitivity Detection of 1 copy Detection of 1 copy
• Your 7500 Fast or 7500 System of template in a 20 µL of template in a 50 µL
can distinguish between samples reaction for a single reaction for a single
containing 5,000–10,000 DNA template reporter TaqMan assay,
®

reporter TaqMan assay,

®

copies, with a statistical confidence level with 99.7% confidence. with 99.7% confidence.
of 99.7%
Run Time < 30 minutes < 2 hours
• A 7500 Fast System can complete this
analysis in as little as 30 minutes
System Specifications

Thermal Cycling System Peltier-based, 96-well block

Optical System CCD camera with halogen lamp excitation;

five-excitation and five-emission filters

“I was very impressed Calibrated Dyes at Installation FAM /SYBR Green, VIC /JOE , NED /TAMRA /Cy3 ,
TM ® ® TM TM TM ®

ROX /Texas Red , and Cy5

TM ® ®

with the performance of

Additional Dye Available Calibration for new dyes within the wavelength range
the Applied Bisosystems ® is possible by following the custom dye calibration
procedure in the User’s Manual. Purchase of
7500 Fast instrument additional filter sets is not necessary.

coupled with Applied Passive Reference Dyes ROX dye or any calibrated dye.
Use of a passive reference dye is optional.
Bisosystems Fast PCR ®

Reaction Volumes 5–30 µL 20–100 µL

Master Mix. Conversion Sample Format Fast 96-well plates Standard 96-well plates
optimized for 10 µL reactions and 0.2 mL tubes
from our standard real time
Peak Block Heating Rate 5.5°C 2.5°C
PCR procedure was very
Temperature Range 4–100°C 4–100°C
simple and straightforward. Temperature Accuracy +/- 0.25°C of setpoint/display temperature,

We saw an increase in measured at 3 minutes after clock start

Temperature Uniformity +/- 0.50°C, 30 seconds after clock start

sensitivity, and the run only
Dimensions (w x d x h) 34 cm (13.99 in) x 45 cm (17.72 in) x 49 cm (19.29 in)
took about 35 minutes.”
Weight 34 kg (75 lb)
Karen, Associate Scientist,
Veterinary Diagnostic Laboratory Software Specifications

Applications Comparative Ct, Standard Curve, Relative Standard

Curve, Allelic Discrimination, Plus/Minus

Dye Discrimination Multicomponenting algorithm

Multiplate Data Comparison Compare an unlimited number plates of gene

expression assays

Multiplex Capability Multiplex up to five targets per well

ORDERING INFORMATION

Description Part Number

7500 Real-Time PCR System with Notebook Computer and SDS Software 4351104

7500 Real-Time PCR System with Tower Computer and SDS Software 4351105

7500 Fast System Upgrade Kit 4362143

7500 Fast Real-Time PCR System with Notebook Computer and SDS Software 4351106

7500 Fast Real-Time PCR System with Tower Computer and SDS Software 4351107

Notebook Computer for 7300/7500/7500 Fast System 4359286

Tower Computer for 7300/7500/7500 Fast System 4359284

7500 System SDS v1.4 Software 21 CFR Part 11 Module 4377354

7500 Fast System SDS v1.4 Software 21 CFR Part 11 Module 4377355

Service and Support

Applied Biosystems has the most extensive network of
Field Application Specialist (FAS) and Support Engineers
dedicated to real-time PCR.

Included with the purchase of a 7500 Fast

or 7500 System:
• Full instrument installation and performance
verification by a certified Field Service Engineer
• One day of FAS training
• One-year warranty

Additionally, Applied Biosystems has a portfolio of flexible

service options designed with your lab in mind.

The Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems are covered by US Patent No. 6,814,934, EP876562, and other patents owned by Applied Biosystems. For
further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
© 2009 Life Technologies Corporation. All rights reserved. Trademarks of Life Technologies Corporation and its affiliated companies: Applied Biosystems®, AB Logo™, FAM™, JOE™,
NED™, ROX™, SYBR®, Texas Red®, TAMRA™ and VIC®. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Cy3 and Cy5 are registered trademarkes of GE Healthcare
Bio-Sciences. Windows and Vista are registered trademarks of Microsoft Corporation in the United States and other countries. All other trademarks are the sole property of their
respective owners.
Printed in the USA, 08/2009 Publication 117SP05-10 B-088026

Headquarters International Sales

850 Lincoln Centre Drive | Foster City, CA 94404 USA For our office locations please call the division
Phone 650.638.5800 | Toll Free 800.345.5224 headquarters or refer to our Web site at
www.appliedbiosystems.com www.appliedbiosystems.com/about/offices.cfm