SAFETY DATA SHEET Version 6.

2
Revision Date 07.08.2019
Print Date 04.10.2019

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifiers
Product name : Hexane
Product Number : 296090
Brand : Sigma-Aldrich
CAS-No. : 110-54-3
1.2 Other means of identification
n-Hexane
1.3 Relevant identified uses of the substance or mixture and uses advised against
Identified uses : For R&D use only. Not for pharmaceutical, household or other
uses.
1.4 Details of the supplier of the safety data sheet
Company : Sigma-Aldrich (M) Sdn. Bhd.
A-07-11 Empire Office, Empire Subang
47500 SUBANG JAYA, SELANGOR DARUL EHSAN
MALAYSIA
Telephone : +60 603 563-53321
Fax : +60 603 563-54116
1.5 Emergency telephone number
Emergency Phone # : +62 08001401253

SECTION 2: Hazards identification

2.1 GHS Classification

Classification according to CLASS regulations 2013
Flammable liquids (Category 2), H225
Skin corrosion/irritation (Category 2), H315
Reproductive toxicity (Category 2), H361f
Specific target organ toxicity - single exposure (Category 3), Central nervous system, H336
Specific target organ toxicity - repeated exposure (Category 2), H373
Aspiration hazard (Category 1), H304
Hazardous to the aquatic environment - chronic hazard (Category 2), H411
For the full text of the H-Statements mentioned in this Section, see Section 16.
2.2 GHS Label elements, including precautionary statements
Labelling according to CLASS regulations 2013
Pictogram

Signal word Danger

Sigma-Aldrich- 296090 Page 1 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
 Hazard statement(s)
H225 Highly flammable liquid and vapour.
H304 May be fatal if swallowed and enters airways.
H315 Causes skin irritation.
H336 May cause drowsiness or dizziness.
H361f Suspected of damaging fertility.
H373 May cause damage to organs through prolonged or repeated
exposure.
H411 Toxic to aquatic life with long lasting effects.
Precautionary statement(s)
Prevention
P210 Keep away from heat/sparks/open flames/hot surfaces. No
smoking.
P260 Do not breathe dust/ fume/ gas/ mist/ vapours/ spray.
P273 Avoid release to the environment.
P281 Use personal protective equipment as required.
Response
P301 + P310 IF SWALLOWED: Immediately call a POISON CENTER or doctor/
physician.
P331 Do NOT induce vomiting.
P370 + P378 In case of fire: Use dry sand, dry chemical or alcohol-resistant
foam for extinction.
P391 Collect spillage.
2.3 Other hazards - none

SECTION 3: Composition/information on ingredients

Substance / Mixture : Substance

3.1 Substances
Synonyms : n-Hexane

Formula : C6H14
Molecular weight : 86.18 g/mol
CAS-No. : 110-54-3
EC-No. : 203-777-6
Index-No. : 601-037-00-0
Hazardous components
Component Classification Concentration
n-Hexane
Flam. Liq. 2; 2; Repr. 2; <= 100 %
STOT SE 3; STOT RE 2;
Asp. Tox. 1; Aquatic
Chronic 2; H225, H315,
H361f, H336, H373, H304,
H411
Concentration limits:
>= 5 %: STOT RE 2,
H373; >= 20 %: STOT SE
3, H336;

Sigma-Aldrich- 296090 Page 2 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
 For the full text of the H-Statements mentioned in this Section, see Section 16.

SECTION 4: First aid measures

4.1 Description of first aid measures

General advice
Consult a physician. Show this safety data sheet to the doctor in attendance.
If inhaled
If breathed in, move person into fresh air. If not breathing, give artificial respiration.
Consult a physician.
In case of skin contact
Wash off with soap and plenty of water. Consult a physician.
In case of eye contact
Flush eyes with water as a precaution.
If swallowed
Do NOT induce vomiting. Never give anything by mouth to an unconscious person. Rinse
mouth with water. Consult a physician.
4.2 Most important symptoms and effects, both acute and delayed
The most important known symptoms and effects are described in the labelling (see section
2.2) and/or in section 11
4.3 Indication of any immediate medical attention and special treatment needed
No data available

SECTION 5: Firefighting measures

5.1 Extinguishing media

Suitable extinguishing media
Dry powder Dry sand
Unsuitable extinguishing media
Do NOT use water jet.
5.2 Special hazards arising from the substance or mixture
Carbon oxides
5.3 Advice for firefighters
Wear self-contained breathing apparatus for firefighting if necessary.
5.4 Further information
Use water spray to cool unopened containers.

SECTION 6: Accidental release measures

6.1 Personal precautions, protective equipment and emergency procedures

Use personal protective equipment. Avoid breathing vapours, mist or gas. Ensure
adequate ventilation. Remove all sources of ignition. Evacuate personnel to safe areas.
Beware of vapours accumulating to form explosive concentrations. Vapours can
accumulate in low areas.
For personal protection see section 8.

Sigma-Aldrich- 296090 Page 3 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
6.2 Environmental precautions
Prevent further leakage or spillage if safe to do so. Do not let product enter drains.
Discharge into the environment must be avoided.
6.3 Methods and materials for containment and cleaning up
Contain spillage, and then collect with non-combustible absorbent material, (e.g. sand,
earth, diatomaceous earth, vermiculite) and place in container for disposal according to
local / national regulations (see section 13).
6.4 Reference to other sections
For disposal see section 13.

SECTION 7: Handling and storage

7.1 Precautions for safe handling

Avoid contact with skin and eyes. Avoid inhalation of vapour or mist.
Flash back possible over considerable distance.Container explosion may occur under fire
conditions.Keep away from sources of ignition - No smoking.Take measures to prevent the
build up of electrostatic charge.
For precautions see section 2.2.
7.2 Conditions for safe storage, including any incompatibilities
Keep container tightly closed in a dry and well-ventilated place. Containers which are
opened must be carefully resealed and kept upright to prevent leakage.
7.3 Specific end use(s)
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated

SECTION 8: Exposure controls/personal protection

8.1 Control parameters

Components with workplace control parameters
Component CAS-No. Value Control Basis
parameters
n-Hexane 110-54-3 TWA 50 ppm 176 Malaysia. Occupational Safety
mg/m3 and Health (Use and Standards
of Exposure of Chemicals
Hazardous to Health)
Regulations 2000.
Remarks Skin
8.2 Exposure controls
Appropriate engineering controls
Handle in accordance with good industrial hygiene and safety practice. Wash hands
before breaks and at the end of workday.
Personal protective equipment
Eye/face protection
Face shield and safety glasses Use equipment for eye protection tested and
approved under appropriate government standards such as NIOSH (US) or EN
166(EU).
Skin protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove
removal technique (without touching glove's outer surface) to avoid skin contact
Sigma-Aldrich- 296090 Page 4 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
 with this product. Dispose of contaminated gloves after use in accordance with
applicable laws and good laboratory practices. Wash and dry hands.
The selected protective gloves have to satisfy the specifications of Regulation (EU)
2016/425 and the standard EN 374 derived from it.
Full contact
Material: Nitrile rubber
Minimum layer thickness: 0.4 mm
Break through time: 480 min
Material tested:Camatril® (KCL 730 / Aldrich Z677442, Size M)
Splash contact
Material: Nitrile rubber
Minimum layer thickness: 0.2 mm
Break through time: 59 min
Material tested:Dermatril® P (KCL 743 / Aldrich Z677388, Size M)
data source: KCL GmbH, D-36124 Eichenzell, phone +49 (0)6659 87300, e-mail
sales@kcl.de, test method: EN374
If used in solution, or mixed with other substances, and under conditions which
differ from EN 374, contact the supplier of the CE approved gloves. This
recommendation is advisory only and must be evaluated by an industrial hygienist
and safety officer familiar with the specific situation of anticipated use by our
customers. It should not be construed as offering an approval for any specific use
scenario.
Body Protection
Complete suit protecting against chemicals, Flame retardant antistatic protective
clothing., The type of protective equipment must be selected according to the
concentration and amount of the dangerous substance at the specific workplace.
Respiratory protection
Where risk assessment shows air-purifying respirators are appropriate use a full-
face respirator with multi-purpose combination (US) or type AXBEK (EN 14387)
respirator cartridges as a backup to engineering controls. If the respirator is the sole
means of protection, use a full-face supplied air respirator. Use respirators and
components tested and approved under appropriate government standards such as
NIOSH (US) or CEN (EU).
Control of environmental exposure
Prevent further leakage or spillage if safe to do so. Do not let product enter drains.
Discharge into the environment must be avoided.

SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties
a) Appearance Form: liquid
Colour: colourless
b) Odour hydrocarbon-like
c) Odour Threshold No data available
d) pH 7.0
e) Melting Melting point/range: -95 °C
point/freezing point

Sigma-Aldrich- 296090 Page 5 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
 f) Initial boiling point 69 °C
and boiling range
g) Flash point -22 °C - c.c.
h) Evaporation rate 15.8
i) Flammability (solid, No data available
gas)
j) Upper/lower Upper explosion limit: 8.1 %(V)
flammability or Lower explosion limit: 1.0 %(V)
explosive limits
k) Vapour pressure 100 hPa at 9.8 °C
l) Vapour density No data available
m) Relative density 0.659 g/mL at 25 °C
n) Water solubility 0.01 g/l at 25 °C - slightly soluble
o) Partition coefficient: log Pow: ca.4 at 20 °C - (Lit.), Potential bioaccumulation
n-octanol/water
p) Auto-ignition 225 °C
temperature at 1,013 hPa
q) Decomposition No data available
temperature
r) Viscosity No data available
s) Explosive properties No data available
t) Oxidizing properties No data available
9.2 Other safety information
No data available

SECTION 10: Stability and reactivity

10.1 Reactivity
No data available
10.2 Chemical stability
Stable under recommended storage conditions.
10.3 Possibility of hazardous reactions
No data available
10.4 Conditions to avoid
Exposure to moisture may affect product quality.
Heat, flames and sparks.
10.5 Incompatible materials
Strong oxidizing agents, Rubber, various plastics
10.6 Hazardous decomposition products
Other decomposition products - No data available
Hazardous decomposition products formed under fire conditions. - Carbon oxides
In the event of fire: see section 5

Sigma-Aldrich- 296090 Page 6 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
SECTION 11: Toxicological information
11.1 Information on toxicological effects
Acute toxicity
LD50 Oral - Rat - male and female - 16,000 mg/kg
(OECD Test Guideline 401)
LC50 Inhalation - Rat - 4 h - 172 mg/l
Remarks: (RTECS)
LD50 Dermal - Rabbit - male - > 2,000 mg/kg
(OECD Test Guideline 402)
Remarks: (ECHA)
Skin corrosion/irritation
Skin - Rabbit
Result: Skin irritation - 24 h
(OECD Test Guideline 404)
Remarks: (Regulation (EC) No 1272/2008, Annex VI)
Serious eye damage/eye irritation
Eyes - Rabbit
Result: No eye irritation - 72 h
(OECD Test Guideline 405)
Respiratory or skin sensitisation
Local lymph node assay (LLNA) - Mouse
Result: negative
(OECD Test Guideline 429)
Germ cell mutagenicity
Ames test
Salmonella typhimurium
Result: negative

Mouse - male
Result: negative
(ECHA)
Carcinogenicity
IARC: No component of this product present at levels greater than or equal to 0.1% is
identified as probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
Suspected of damaging fertility.
Specific target organ toxicity - single exposure
May cause drowsiness or dizziness. - Central nervous system
Classified according to Regulation (EU) 1272/2008, Annex VI (Table 3.1/3.2)
Specific target organ toxicity - repeated exposure
Inhalation - May cause damage to organs through prolonged or repeated exposure. -
Nervous system
Classified according to Regulation (EU) 1272/2008, Annex VI (Table 3.1/3.2)
Aspiration hazard
May be fatal if swallowed and enters airways.
Aspiration hazard, Aspiration may cause pulmonary oedema and pneumonitis.
Additional Information
Repeated dose toxicity - Rat - male - Oral - No observed adverse effect level - 6.6 mg/kg

Sigma-Aldrich- 296090 Page 7 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
 (ECHA)
RTECS: MN9275000

Drowsiness, irritant effects, somnolence

narcosis, Nausea, Tiredness, CNS disorders, paralysis symptoms
Risk of corneal clouding.
It generally applies for aliphatic hydrocarbons with 6 - 18 carbon atoms t pulmonary
oedema, upon direct inhalation, i.e. in conditions that can occ spraying, inhalation of
aerosols and similar). After absorption of very l
To the best of our knowledge, the chemical, physical, and toxicological properties have not
been thoroughly investigated.

SECTION 12: Ecological information

12.1 Toxicity
Toxicity to fish LC50 - Pimephales promelas (fathead minnow) - 2.5 mg/l - 96 h
Remarks: (ECOTOX Database)
Toxicity to daphnia EC50 - Daphnia magna (Water flea) - 2.1 mg/l - 48 h
and other aquatic Remarks: (Lit.)
invertebrates
12.2 Persistence and degradability
Biodegradability aerobic - Exposure time 28 d
Result: 98 % - Readily biodegradable.
(OECD Test Guideline 301F)
Remarks: (in analogy to similar products)
12.3 Bioaccumulative potential
No data available
12.4 Mobility in soil
No data available
12.5 Results of PBT and vPvB assessment
PBT/vPvB assessment not available as chemical safety assessment not required/not
conducted
12.6 Other adverse effects
Toxic to aquatic life with long lasting effects.
No data available

SECTION 13: Disposal considerations

13.1 Waste treatment methods

Product
Offer surplus and non-recyclable solutions to a licensed disposal company. Burn in a
chemical incinerator equipped with an afterburner and scrubber but exert extra care in
igniting as this material is highly flammable.
Contaminated packaging
Dispose of as unused product.

Sigma-Aldrich- 296090 Page 8 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
SECTION 14: Transport information
14.1 UN number
ADR/RID: 1208 IMDG: 1208 IATA-DGR: 1208
14.2 UN proper shipping name
ADR/RID: HEXANES
IMDG: HEXANES
IATA-DGR: Hexanes
14.3 Transport hazard class(es)
ADR/RID: 3 IMDG: 3 IATA-DGR: 3
14.4 Packaging group
ADR/RID: II IMDG: II IATA-DGR: II
14.5 Environmental hazards
ADR/RID: yes IMDG Marine pollutant: yes IATA-DGR: no
14.6 Special precautions for user
None
14.7 Incompatible materials
Strong oxidizing agents, Rubber, various plastics

SECTION 15: Regulatory information

15.1 Safety, health and environmental regulations/legislation specific for the

substance or mixture
Notification status
AICS: On the inventory, or in compliance with the inventory
DSL: All components of this product are on the Canadian DSL
ENCS: On the inventory, or in compliance with the inventory
ISHL: On the inventory, or in compliance with the inventory
KECI: On the inventory, or in compliance with the inventory
NZIoC: On the inventory, or in compliance with the inventory
PICCS: On the inventory, or in compliance with the inventory

SECTION 16: Other information

Full text of H-Statements referred to under sections 2 and 3.

H225 Highly flammable liquid and vapour.
H304 May be fatal if swallowed and enters airways.
H315 Causes skin irritation.
H336 May cause drowsiness or dizziness.
H361f Suspected of damaging fertility.
H373 May cause damage to organs through prolonged or repeated exposure.
H411 Toxic to aquatic life with long lasting effects.

Sigma-Aldrich- 296090 Page 9 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada
 Further information
Copyright 2018 Sigma-Aldrich Co. LLC. License granted to make unlimited paper copies
for internal use only.
The above information is believed to be correct but does not purport to be all inclusive
and shall be used only as a guide. The information in this document is based on the
present state of our knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any guarantee of the properties of
the product. Sigma-Aldrich Corporation and its Affiliates shall not be held liable for any
damage resulting from handling or from contact with the above product. See
www.sigma-aldrich.com and/or the reverse side of invoice or packing slip for additional
terms and conditions of sale.

The branding on the header and/or footer of this document may temporarily not visually
match the product purchased as we transition our branding. However, all of the
information in the document regarding the product remains unchanged and matches the
product ordered. For further information please contact mlsbranding@sial.com.

Sigma-Aldrich- 296090 Page 10 of 10

The life science business of Merck operates as MilliporeSigma in

the US and Canada