The document discusses exposure to agents that damage DNA such as physical, chemical, and biological factors. These agents can cause errors in DNA replication, infections, and environmental factors that induce DNA lesions. The cellular response involves DNA damage checkpoints, sensors and effectors that activate DNA repair pathways to resolve DNA damage and maintain genomic integrity.
The document discusses exposure to agents that damage DNA such as physical, chemical, and biological factors. These agents can cause errors in DNA replication, infections, and environmental factors that induce DNA lesions. The cellular response involves DNA damage checkpoints, sensors and effectors that activate DNA repair pathways to resolve DNA damage and maintain genomic integrity.
The document discusses exposure to agents that damage DNA such as physical, chemical, and biological factors. These agents can cause errors in DNA replication, infections, and environmental factors that induce DNA lesions. The cellular response involves DNA damage checkpoints, sensors and effectors that activate DNA repair pathways to resolve DNA damage and maintain genomic integrity.
The document discusses exposure to agents that damage DNA such as physical, chemical, and biological factors. These agents can cause errors in DNA replication, infections, and environmental factors that induce DNA lesions. The cellular response involves DNA damage checkpoints, sensors and effectors that activate DNA repair pathways to resolve DNA damage and maintain genomic integrity.
C Regulators in the DNA Damage Response Apoptosis Cell cycle DNA repair Metabolism Senescence
AC Yunhua Liu, Yujing Li, Xiongbin Lu checkpoints
PII: S0003-9861(16)30037-6 DOI: 10.1016/j.abb.2016.02.018 Reference: YABBI 7205 ü ERRORES EN EL PROCESO DE REPLICACIÓN ü INFECCIONES ü FACTORES AMBIENTALES
u SINDROMES DE INESTABILIDAD CROMOSÓMICA u CÁNCER
ALTERACIONES EN LOS SISTEMAS
DE REPARACIÓN v AGENTES MUTAGÉNICOS v ESPONTÁNEOS
ü DESAMINACIÓN (PÉRDIDA DE GRUPOS AMINO)
CITOSINA PRODUCE URACILO
CONVERSIÓN DE GC X AT
ü DEPURINIZACIÓN (ELIMINACIÓN DEL ENLACE N-
GLUCOSIDICO)
BASE Y AZÚCAR SE PIERDE LA ADENINA O GUANINA
ü DAÑO OXIDATIVO ü FORMACIÓN DE 8 OXO guanosina y el glicol de timina u Agentes alquilantes Cell Stem Cell
Review o Añaden grupos etilo o DNA DAMAGING DNA DNA REPAIR
metilo AGENTS LESIONS PATHWAYS FIDELITY
Non Homologous Ionizing radiation C Double strand breaks End Joining (NHEJ) + Single strand breaks X-rays C Intrastrand crosslinks Anti-tumor drugs C Homologous Interstrand crosslinks ++ o A las bases C Recombination (HR)
o Alteran el patrón de Nucleotide UV-light Bulky adducts chemicals T Pyrimidine dimers Excision +++ Repair (NER) T
apareamiento Oxygen radicals Abasic sites Base Excision Hydrolysis 8-oxo Single strand breaks Repair (BER) +++ Alkylating agents G 8-oxoguanine lesions
T C A G Bases mismatch Replication errors T Insertions Mismatch Repair (MMR) +++ A Deletions
Figure 1. DNA-Repair Pathways in Mammalian Cells
Each type of DNA assault results in a different type of lesion, which can be repaired with different fidelity by distinct and highly specialized repair pathways. Agentes intercalantes
u PROFLAVINA u ACRIDINA u ETIDIO
http://evolucionbiologica- apuntes.blogspot.com/2015/01/reparacion-del-adn.html Efecto de la radiación ultr ENERGÍA IONIZANTE
PRODUCCIÓN DE l p DÍMEROS DE m PIRIMIDINAS h c n 7 TIMINAS Dímeros de pirimidina r r (6-4) fotoproductos p ( Figura 1. Estructuras de lesiones en el ADN inducidas por “ radiación UV-B. d c l ADN polimerasa como la ARN polimerasa no son f REPARACIÓN DIRECTA
ELIMINACIÓN DIRECTA DEL DAÑO AL DNA
IRREVERSIBLES PROCARIONTES
EZIMA FOTOLIASA ALQUILTRASFERASA https://pt.slideshare.net/JuanPabloFabian/reparacion-de-adn-80634224/3 REPARACIÓN DEL DNA • BER: SITIOS DONDE SE ENCUENTRAN CITOSINAS, ADENINAS O GUANINAS DESAMINADAS, ALQUILADAS POR AGENTES EXOGENOS (8-OXOG) • NER: AGRUPA VARIAS PROTEÌNAS QUE RECONOCEN LA LESIÓN, SIRVEN DE CONTACTO PARA NUCLEASAS RETIRAN DE 12-30 NUCLEOTIDOS • MMR: QUITA NUCLEOTIDOS INSERTADOS ERRONEAMENTE POR LA POLIMERASA
• DBR: ACTÚA POR EXPOSICIÓN A RADIACIONES
IONIZANTES O QUÍMICOS, LA UNIÓN DE EXTREMOS NO HOMÓLOGOS SISTEMA 8 0XO GUANINA
