The exceptional xylanase enzyme

DSM Nutritional Products

RONOZYME ® WX is an outstanding xylanase RONOZYME® WX is derived from Thermomyces
designed to meet the requirements of modern poultry lanuginosus spp., an organism that thrives in hot, dry
and pig feeding. Characterised by exceptional heat conditions. This benefit is passed on, ensuring that the
stability, a broad pH range and a high, consistent level enzyme can remain active even at high temperatures.
of xylanase activity, RONOZYME® WX can be used as
the sole NSP enzyme in diets based on wheat, rye and RONOZYME ® WX is an endoxylanase which
triticale or these cereals in combination with barley hydrolyses arabinoxylans and xylans in feeds.
and cereal by-products.

Figure 1: Types and levels of NSP present in some cereal grains (% DM)

18
Others
16
Cellulose
14 Beta-Glucan (sol.)
NSP content (% DM)

12 Beta-Glucan (insol.)

Arabinoxylan (sol.)
10
Arabinoxylan (insol.)
8
6

4
2

0 (Adapted from Choct, 1997)

Rice Sorghum Maize Wheat Rye Triticale Barley
(pearled)
 Plant polysaccharides (long-chain carbo- Soluble arabinoxylans
hydrates) play an important role in feedstuffs.
They can function as a major nutritional Soluble arabinoxylans can increase the
component in the diet (e.g. starch) but a viscosity, or gel-formation, of the intestinal
number of non-starch polysaccharides (NSP's) contents therefore impairing nutrient uptake.
are detrimental to non-ruminants such as poul- Unimpeded movement of the animal’s own
try and pigs. Poultry and pigs are not capable enzymes, feed and products of digestion by
of digesting these NSP's due to a lack of the diffusion in the gut is essential for rapid
relevant enzymes in their digestive tracts. digestion. However, as the viscosity of the
gut contents increases, the rate of diffusion
The term NSP covers a large variety of poly- decreases, hence reducing growth rates,
saccharide molecules. The types and amounts feed efficiency and the apparent
Arabinoxylans

present in cereals (see Figure 1) and vegetable metabolisable energy of the diet. The slower
protein sources vary considerably not only rate of feed transit can also create an envi-
between different ingredients but also within ronment suitable both for intestinal microflora
the same ingredient due to variety and to multiply and compete with the host for
geographical location where grown. This con- nutrients and for pathogenic organisms
sequently affects the overall antinutritive effect. which can lead to additional problems.

O O O O O
Insoluble arabinoxylans
OH
O O
OH
O O
OH Insoluble arabinoxylans within plant cell walls
OH
O
OH
O O
OH
are associated with the encapsulation of
O O O nutrients such as starch and protein. This
HOH2C
OH
HOH2C
OH
HOH2C
OH
allows valuable nutrients to by-pass digestion
OH OH OH
by the animal. Digestion of the insoluble ara-
binoxylans results in improved exposure of
Non-starch polysaccharides include the nutrients. However, once soluble, these
arabinoxylans which are present in both soluble NSPs can produce viscous solutions as
and insoluble forms depending molecular chain described.
length and amount of branching on that chain.

Although arabinoxylans are most typically Dual-action breakdown

associated with wheat and wheat by-products, of anti-nutritional factors
it should be recognised that they are also
present in other raw materials commonly used Generally, xylanases have an affinity for either
in poultry and pig feeds. the soluble or insoluble NSPs, showing a
preferential ability to degrade one form or
RONOZYME® WX will degrade the arabin- the other. However, in contrast to many
oxylans in the feed irrespective of the source. other xylanase enzyme products on the
market, RONOZYME ® WX has a broad
spectrum of activity against both the soluble
and insoluble arabinoxylans in the feed.

RONOZYME® WX can therefore be used

successfully to increase both the productivity
and profitability of animals fed diets contain-
ing arabinoxylan-rich cereals and cereal
by-products.
– the more heat stable, more reliable

In recent years, the degree and duration of Enzyme protein structure

hydro-thermal processing applied during feed
production in many feedmills has been increased,
particularly to improve the hygiene status of the feed
(e.g. salmonella kill).

Enzymes are proteins that have specific and complex

structures. These structures are essential for the
activity of enzymes and if denatured, for example by
high temperature (particularly in conjunction with
extended conditioning times), the activity of enzymes
can be reduced.

RONOZYME® WX (CT) is the granulated form with

excellent thermo-stability characteristics, resulting
from the high intrinsic heat stability of the enzyme
combined with the added protection provided by
the unique CT (Coated Thermostable) formulation.
These two factors combine to produce the most The CT granular form
heat-stable xylanase product available on the market.

In addition, the CT formulation is free flowing and

virtually dust free (due to the high quality of the
granules) giving excellent mixing characteristics for
both premixes and feeds.

cm
xylanase product for feeds

RONOZYME® WX (CT) the most RONOZYME® WX (CT) virtually

heat stable enzyme dust-free
The excellent thermo-stability of RONOZYME® WX Dust emission from feed additives can be measured
(CT) is demonstrated in various recovery trials. One of using the standard Heubach filter method. The low-
them was performed at the Biotechnological Institute dusting characteristics of RONOZYME® WX (CT) in
in Kolding, Denmark showing the exceptional stability comparison to some competitor products can be
of RONOZYME® WX (CT) during feed processing clearly demonstrated (Figure 3).
outperforming competitors (Figure 2). The dust free formulation minimizes losses during
feed preparation and reduces the potential for allergic
RONOZYME® WX (CT) is the only enzyme which reactions due to enzyme exposure.
even at 95°C had more than 80% recovered enzyme
activity, whereas less than 15% enzyme activity was
recovered for the competitor products.

Figure 2: Residual activities after pelleting Figure 3: Measured dust by different feed enzymes

100 80
mg dust / 25 g product on Heubach filter
Recovery (as % of activity in mash feed)

70
80
60

60 50

40
40 30

20
20
10

0 0
75° C 85° C 90° C 95° C RON® ROX® Comp. Comp. Comp. Comp. Comp. Comp.
WX G2 G 1 2 3 4 5 6
RONOZYME ® WX Comp. A Comp. B Comp. C
Study VFP 24016, DSM, Kaiseraugst, CH (2004)
Comp. D Comp. E Comp. E

Danish Tech. Institute, Kolding, DK (2004)

For improved performance and profitability
in poultry diets

Effective in poultry
Poultry and Pigs

The effect of RONOZYME ® WX Figure 4

supplementation on performance in
broilers fed a wheat-based diet was 590 1.60

studied at the Research Centre, Foulum, 580

1.56
Denmark (Figure 4). In this trial, 570

Weight gain (g: d 1– 24)

1.52
RONOZYME® WX was added to the feed 560
at 0, 200 and 300 FXU/kg diet. In compar- 550 1.48

FCR
ison with the control diet (no added 540 1.44
enzyme), the addition of RONOZYME®
530
WX resulted in significant (P < 0.05) 1.40
520
improvements in both feed conversion
1.36
ratio (~9.5%) and growth rate (~ 9.5%). 510

Significant reductions in both jejunal and 500 1.32

0 200 300
ileal viscosity resulting from the enzyme RONOZYME WX (g/tonne)
supplementation were also observed.
Weight gain FCR

A similar pattern of results was observed Figure 5

in a trial at the Institut für Klein-
2260 1.92
tierforschung, Merbitz, Germany.
2240
®
RONOZYME WX was added to a wheat- 1.88
2220
based broiler diet at 0, 200 and 300
Weight gain (g: d 1 – 43)

2200
FXU/kg diet. In comparison with the 1.84
2180
control diet, supplementation with
FCR

2160 1.80
RONOZYME® WX resulted in a 2.1%
2140
improvement in FCR and a significant 1.76
(P < 0.05) 5% improvement in weight gain 2120

(Figure 5). As in the previous trial, the 2100

1.72
improvements in performance were asso- 2080

ciated with a significant (P < 0.05) 2060 1.68

0 200 300
reduction in both jejunal and ileal viscosity. RONOZYME WX (g/tonne)

Weight gain FCR

For improved performance and profitability
in pig diets

Effective in pigs

In a trial at the University of Nottingham, UK, Figure 6

piglets (initial weight 8 – 10 kg) were fed a wheat-
based diet supplemented with RONOZYME® WX 605 1.60

at 0, 200 and 400 FXU/kg up to 25 kg liveweight. 600

1.56
595
In comparison with the control group (no enzyme
Daily liveweight gain (g)

590 1.52
addition), the addition of RONOZYME ® WX
585
resulted in improved daily liveweight gain (5% and 1.48
580

FCR
6% respectively for the 2 different treatment 575 1.44
groups) and a significant (P < 0.05) improvement 570
(9%) in feed conversion ratio (Figure 6). 565 1.40
560
1.36
555
550 1.32
0 200 400
RONOZYME WX (g/tonne)
Weight gain FCR

A similar pattern of results was observed in a com- Figure 7

mercial trial conducted in Australia. Piglets (initial
weight 6.8 kg) were fed a wheat-based diet 260 1.52
supplemented with RONOZYME® WX at 0 and 255 1.48
200 FXU/kg diet for a period of 21 days. In com-
Daily liveweight gain (g)

parison with the control group, the addition of 250

1.44
RONOZYME® WX improved both daily liveweight 245
FCR

gain and feed conversion ratio by approximately 1.40

240
8% (Figure 7).
1.36
235

230 1.32

225 1.28
0 200
RONOZYME WX (g/tonne)

Weight gain FCR

The exceptional xylanase enzyme
Product information
RONOZYME® WX is available in two product forms. RONOZYME® WX (CT) a coated
non-dusty light-brown granulate with a minimum xylanase activity of 1000 FXU per g and as
RONOZYME® WX (L) a brown aqueous liquid with a minimum xylanase activity of 650 FXU
per ml.

Recommended dose range*

RONOZYME® WX (CT) RONOZYME® WX (L)
FXU/kg Dose rate FXU/kg Dose rate
complete g/tonne complete ml/tonne
feed complete feed feed complete feed
Broilers 150 – 200 150 – 200 150 – 200 230 – 310
Turkeys 150 – 200 150 – 200 150 – 200 230 – 310
Piglets 150 – 200 150 – 200 150 – 200 230 – 310
9/2005 · GAS.Grenzach@t-online.de

This dosage range is generally recommended, on the basis of cost-effective responses. The actual dosage
may depend on pelleting conditions, desired effects and feed composition such as:

• the inclusion on high levels of cereals e.g. wheat, rye and/or triticale.
• the use of cereals soon after harvesting requires higher dosage (when the soluble NSP content will
be at its highest).
51696

* Regulatory status and dosages for different species may vary between countries.

DSM Nutritional Products Ltd.

P.O. Box 3255
CH-4002 Basel
Switzerland

www.dsmnutritionalproducts.com