PROTEX® 6L

Genencor® Bacterial Alkaline Protease

DESCRIPTION BIOCHEMICAL PARAMETERS

PROTEX® 6L enzyme is a bacterial alkaline Enzyme Type: Alkaline serine endopeptidase
protease derived from a selected strain of Bacillus IUB#: 3.4.21.62
licheniformis. Typical application areas for Molecular Weight: 22.5 kDa
PROTEX® 6L enzyme include protein processing, IEP: 6.8
silver recovery from film material and pet food Activators/cofactors: None required for maximal
production. activity
Inhibitors: Organophosphorous reagents which
TYPICAL CHARACTERISTICS
react with serine (diisopropyl fluorophosphate,
Activity: 580,000 DU/gram (minimum)
diisopropyl phosphofluoridate). Oxidative
Appearance: Amber to light brown liquid
reagents which release active chlorine.
Grade: Food grade, Kosher
APPLICATION RECOMMENDATIONS
Unit Definition
PROTEX® 6L protease is effective in hydrolyzing
The activity of PROTEX® 6L enzyme is expressed
most proteins (hemoglobin, casein, egg yolk,
in DU/gram. The DU assay is based on the ability
gelatin, fish and other plant and animal proteins)
of a protease to cleave p-nitroanilide from a
to lower molecular weight peptides.
synthetic peptide substrate. This results in an
increase in absorbance at 405 nm. This increase in The enzyme expresses more effective hydrolytic
absorbance is related directly to protease activity activity if the substrate is (partially) unfolded or
via the use of enzyme standards. A copy of the denatured protein.
assay method is available upon request.
DOSAGE RECOMMENDATIONS
pH Dependency A typical PROTEX® 6L enzyme dosage rate of 0.25
The pH range for the activity of PROTEX® 6L to 1.00% w/w versus substrate protein is recom-
enzyme is approximately 7.0 to 10.0, with an mended as a starting point for the optimization of
optimum performance at pH 9.5. The exact pH enzyme dosage.
optimum will depend on process variables,
REGULATORY STATUS
including temperature, time, substrate nature and
This product meets or exceeds the Joint FAO/
concentration.
WHO Expert Committee on Food Additives
Temperature Dependency (JECFA) and the Food Chemicals Codex (FCC)
The activity of PROTEX® 6L enzyme is effective in specifications for enzyme preparations used in
the temperature range from 25°C (75°F) to 70°C food and is GRAS.
(160°F), with an optimum performance at 60°C
STORAGE & STABILITY
(140°F). The exact temperature optimum will
PROTEX® 6L enzyme will meet the declared
depend on many process variables, such as pH,
activity of 580,000 DU/g upon arrival at the
time, substrate nature and concentration.
customer’s plant.
Enzyme Inactivation
Genencor® enzymes can be safely stored in
The enzyme can be inactivated by holding for 5 -
unopened and sealed original containers. Enzyme
10 minutes at operational pH, at a temperature of
containers should be stored below 20°C (70°F),
80 - 85°C (175 - 185°F); or at a pH below 4, holding
preferentially refrigerated and sheltered against
for 30 minutes at 50°C (125°F); or at a pH above
direct sunlight.
10, holding for 60 minutes at a temperature of
60°C (140°F). During storage, PROTEX® 6L enzyme will have an
activity loss at elevated temperatures. For more
information on storage of this product, please
contact your Genencor® representative.

Together we can.
PACKAGING
PROTEX® 6L enzyme is available in various package
sizes. Please contact Genencor® for detailed
information.
SAFETY AND ENZYME HANDLING
Inhalation of enzyme dust and mists should be
avoided. In case of contact with the skin or eyes,
promptly rinse with water for at least 15 minutes.
For detailed handling information, please refer
to the appropriate Material Safety Data Sheet;
the Enzyme Technical Association (ETA) handbook,
Working Safely With Enzymes; and the Association
of Manufacturers and Formulators of Enzyme
Products (Amfep) handbook, Guide to the Safe
Handling of Microbial Enzyme Preparations.
All are available from Genencor®.
TECHNICAL SERVICE
Genencor® will work with customers to enhance
processes and solve problems. Let us know what you
need and we will assist you.

For more information

USA and Canada Asia/Pacific
Rochester, New York Singapore
Telephone: +1 800 847 5311 Telephone: +65 6511 5600
Telefax: +1 585 256 5295 Telefax: +65 6511 5666
Europe, Africa, Shanghai, P.R. China
and Middle East Telephone: +86 21 2307 9588
Leiden, The Netherlands Telefax: +86 21 2307 9599
Telephone: +31 71 5686 168 Mumbai, India
Telefax: +31 71 5686 169 Telephone: +91 22 2825 8713
Latin America Telefax: +91 22 2825 8733
Buenos Aires, Argentina
Telephone: +54 11 4875 9500
Telefax: +54 11 4875 9529

© 2009 Danisco US Inc.

GENENCOR and PROTEX are registered trademarks of Danisco US Inc. or its
affiliates in the United States and/or other countries. MULTIFECT, or uses, thereof,
may be covered by one or more issued patents or pending patent applications.
The information contained in this product literature is, to the best of our knowledge,
true and accurate and the product as sold is in conformance with the specifications
set forth herein as determined by the assay methods described. Due to conditions of
use, technical errors or omissions, improper handling or storage beyond our control,
Danisco US Inc. hereby DISCLAIMS ANY EXPRESS OR IMPLIED WARRANTIES,
INCLUDING THE IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS
FOR A PARTICULAR PURPOSE. Danisco US Inc. shall not be liable for any incidental,
consequential, or special damages resulting in any way from the furnishing,
performance, or use of this product literature or the product described herein.
Nothing contained herein shall be construed as a representation by Danisco US Inc.
that the use or resale of the product or processes described herein will not violate
any rules or regulations or infringe upon patents or other intellectual property rights
of third parties or that the recommendations and usage suggestions described
constitute any authorization or inducement to infringe any such rights.
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