Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-Recommended Hand Rub Formulations and Alcohols

Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-
Recommended Hand Rub Formulations and Alcohols
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The Study
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Annika Kratzel, Daniel Todt, Philip V’kovski, Silvio Steiner, Mitra Gultom, Tran Thi
Nhu Thao, Nadine Ebert, Melle Holwerda, Jörg Steinmann, Daniela Niemeyer, Ronald
Dijkman, Günter Kampf, Christian Drosten, Eike Steinmann, Volker Thiel, and
Stephanie Pfaender
Author affiliations: Institute of Virology and Immunology, Bern and Mittelhäusern,
Switzerland (A. Kratzel, P. V’kovski, S. Steiner, M. Gultom, T.T.N. Thao, N. Ebert, M.
Holwerda, R. Dijkman, V. Thiel); University of Bern, Bern (A. Kratzel, P. V’kovski, S.
Steiner, M. Gultom, T.T.N. Thao, N. Ebert, M. Holwerda, R. Dijkman, V. Thiel); Ruhr
University Bochum, Germany (D. Todt, E. Steinmann, S. Pfaender); Institute of Medical
Microbiology, University Hospital of Essen, Essen, Germany (J. Steinmann); Institute of
Clinical Hygiene, Medical Microbiology and Infectiology General Hospital Nürnberg,
Paracelsus Medical University, Nürnberg, Germany (J. Steinmann); Institute of Virology,
Charité Berlin, Berlin, Germany (D. Niemeyer, C. Drosten); Institute for Hygiene and
Environmental Medicine, University Medicine Greifswald, Greifswald, Germany (G. Kampf)
Suggested citation for this article
Abstract
Infection control instructions call for use of alcohol-based hand rub solutions to inactivate
severe acute respiratory syndrome coronavirus 2. We determined the virucidal activity of
World Health Organization–recommended hand rub formulations, at full strength and
multiple dilutions, and of the active ingredients. All disinfectants demonstrated efficient virus
inactivation.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third
highly pathogenic human coronavirus to cross the species barrier into the
human population during the past 20 years (1–3). SARS-CoV-2 infection is
associated with coronavirus disease (COVID-19), which is characterized by
severe respiratory distress, fever, and cough and high rates of mortality,
especially in older persons and those with underlying health conditions (3). The
World Health Organization (WHO) declared SARS-CoV-2 a pandemic on March
11, 2020 (4), and by April 8, a total of 1,447,466 confirmed cases and 83,471
deaths from SARS-CoV-2 had been reported worldwide (5).
Human-to-human transmission of SARS-CoV-2 is efficient, and infected

persons can transmit the virus even when they have no, or only mild,
symptoms (3). Because no antiviral drugs or vaccines are available, virus
containment and prevention of infection are the current highest priorities. To
limit virus spread, effective hand hygiene is crucial. Therefore, easily available
but efficient disinfectants are needed. WHO’s guidelines for hand hygiene in
healthcare suggest 2 alcohol-based formulations for hand sanitization to
reduce the infectivity and spread of pathogens (6). WHO’s recommendations
are based on fast-acting, broad-spectrum microbicidal activity, along with
accessibility and safety. The original WHO formulations failed to meet the
efficacy requirements of European Norm 1500 in previous tests (7). However,
Suchomel et al. (8) suggested modified versions with increased concentrations
of ethanol: 80% (wt/wt) (85.5% [vol/vol]; formulation I), or isopropanol, 75%
(wt/wt) (81.3% [vol/vol]; formulations II). Later, they complemented these by
reducing the glycerol concentrations (9).
We previously showed that these modified WHO formulations were able to

inactivate severe acute respiratory syndrome coronavirus (SARS-CoV) and
Middle East respiratory syndrome coronavirus (MERS-CoV; 10), which are
related to SARS-CoV-2. Current recommendations to inactivate SARS-CoV-2
were translated from findings of other coronaviruses (11). To evaluate whether
these alcohol-based disinfectants also effectively inactivate SARS-CoV-2, we
tested different concentrations of the original and modified WHO formulations
I and II (6,9), ethanol, and 2-propanol for virucidal activity.
The Study
We propagated SARS-CoV-2 (SARS-CoV-2/München-1.1/2020/929) on VeroE6

cells (kindly provided by M. Müller and C. Drosten; Charité, Berlin, Germany).
We cultured VeroE6 cells in Dulbecco’s modified minimal essential medium
supplemented with 10% heat inactivated fetal bovine serum, 1% nonessential
amino acids, 100 µg/mL of streptomycin and 100 IU/mL of penicillin, and 15
mMol of HEPES (Gibco; ThermoFisher, https://www.thermofisher.comExternal
Link).
Original WHO formulation I consists of 80% (vol/vol) ethanol, 1.45% (vol/vol)

glycerol, and 0.125% (vol/vol) hydrogen peroxide. Original WHO formulation II
consists of 75% (vol/vol) 2-propanol, 1.45% (vol/vol) glycerol, and 0.125%
(vol/vol) hydrogen peroxide. The modified WHO formulation I used in our
study consists of 80% (wt/wt) ethanol, 0.725% (vol/vol) glycerol, and 0.125%
(vol/vol) hydrogen peroxide. The modified isopropyl-based WHO formulation II
contains 75% (wt/wt) 2-propanol, 0.725% (vol/vol) glycerol, and 0.125%
(vol/vol) hydrogen peroxide (9). We also prepared ethanol (CAS 64–17–5) and
2-propanol (CAS 67–63–0) in vol/vol dilutions for investigation.
We performed virucidal activity studies by using a quantitative suspension test

with 30 s exposure time (6). In brief, we mixed 1 part virus suspension with 1
part organic load (0.3% bovine serum albumin as an interfering substance) and
8 parts disinfectant solution of different concentrations. After a 30 s exposure,
we serially diluted samples and determined the 50% tissue culture infectious
dose (TCID ) per milliliter by using crystal violet staining and subsequently
50
scoring the number of wells displaying cytopathic effects. We calculated

TCID by the Spearman-Kärber algorithm, as described (12). We monitored the
50
cytotoxic effects of disinfectants by using crystal violet staining and optical

analysis for altered density and morphology of the cellular monolayer in the
absence of virus. We quantified cytotoxic effects analogous to the TCID /mL of
50
the virus infectivity.
We determined dose-response curves as percent normalized virus inactivation

versus percent log disinfectant concentration by nonlinear regression using the
robust fitting method on the normalized TCID data implemented in Prism
50
version 8.0.3 (GraphPad, https://www.graphpad.comExternal Link). We plotted
reference curves for SARS-CoV, MERS-CoV, and bovine CoV (BCoV) by using
previously published data (9). BCoV is often used as surrogate for highly
pathogenic human CoVs. We assessed the mean TCID and standard 50
deviations of means from 3 individual experiments. We identified outliers by

using Grubb’s test in Prism. We calculated reduction factors (RFs) for each
treatment condition as follows:
Figure 1. Virucidal activity of original and modified World Health Organization (WHO)–recommended hand rub
formulations I and II for inactivating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The means of
3 independent experiments...
Our results showed that SARS-CoV-2 was highly susceptible to the original and
the modified WHO formulations (Figure 1). The original and modified versions
of formulation I efficiently inactivated the virus. The original formulation I of
80% (vol/vol) ethanol had an RF of >3.8 (Figure 1, panel A) and the modified
formulation I of 80% (wt/wt) ethanol had an RF of >5.9 (Figure 1, panel C).
Dilutions >40% were still effective (Figure 1, panels A and C). Subsequent
regression analysis of modified formulation I revealed similar inactivation
profiles compared with SARS-CoV, MERS-CoV, and BCoV. (Figure 1, panel C).
The original and modified versions of formulation II also were effective. The
original formulation II of 75% (vol/vol) 2-propanol had a log -reduction of >3.8 10
(Figure 1, panel B) and the modified formulation II of 75% (wt/wt) 2-propanol

had a log -reduction of >5.9. Dilution >30% (vol/vol) also resulted in complete
10
viral inactivation (Figure 1, panel D). Regression analysis of modified WHO

formulation II showed the inactivation profile of SARS-CoV-2 was comparable
to those of SARS-CoV, BCoV, and MERS-CoV (Figure 1, panel D).
Figure 2. Effect of commercially available alcohols in inactivating SARS-CoV-2. The means of 3 independent
experiments with SDs (error bars) are shown. A) Results for ethanol. B) Results for 2-propanol. Dark gray bar...
We also investigated the susceptibility of SARS-CoV-2 against the active
components of the WHO-recommended formulations, which are also the
active ingredients of commercially available hand disinfectants. Ethanol (Figure
2, panel A) and 2-propanol (Figure 2, panel B) were able to reduce viral titers to
background levels in 30 s with RFs of between 4.8 and ≥5.9. Furthermore, we
noted that a concentration of >30% (vol/vol) ethanol or 2-propanol is
sufficient for complete viral inactivation (Figure 2).
Top
Conclusions
We found that SARS-CoV-2 was efficiently inactivated by WHO-recommended

formulations, supporting their use in healthcare systems and viral outbreaks.
Of note, both the original and modified formulations were able to reduce viral
titers to background level within 30 s. In addition, ethanol and 2-propanol
were efficient in inactivating the virus in 30 s at a concentration of >30%
(vol/vol). Alcohol constitutes the basis for many hand rubs routinely used in
healthcare settings. One caveat of this study is the defined inactivation time of
exactly 30 s, which is the time recommended but not routinely performed in
practice. Our findings are crucial to minimize viral transmission and maximize
virus inactivation in the current SARS-CoV-2 outbreak.
Top
Ms. Kratzel is a PhD candidate affiliated with the Institute of Virology and
Immunology, Bern and Mittelhäusern, Switzerland, the Department of
Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern,
Bern, Switzerland and the Graduate School for Cellular and Biomedical
Sciences, University of Bern. Her research interests include coronaviruses.

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Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-

Recommended Hand Rub Formulations and Alcohols

Autopsy of Patient with COVID-19, Japan

More articles on Coronavirus, COVID-19

Human-to-human transmission of SARS-CoV-2 is efficient, and infected

We previously showed that these modified WHO formulations were able to

We propagated SARS-CoV-2 (SARS-CoV-2/München-1.1/2020/929) on VeroE6

Original WHO formulation I consists of 80% (vol/vol) ethanol, 1.45% (vol/vol)

We performed virucidal activity studies by using a quantitative suspension test

scoring the number of wells displaying cytopathic effects. We calculated

cytotoxic effects of disinfectants by using crystal violet staining and optical

the virus infectivity.

We determined dose-response curves as percent normalized virus inactivation

deviations of means from 3 individual experiments. We identified outliers by

(Figure 1, panel B) and the modified formulation II of 75% (wt/wt) 2-propanol

viral inactivation (Figure 1, panel D). Regression analysis of modified WHO

We found that SARS-CoV-2 was efficiently inactivated by WHO-recommended

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