CY009

English
CYANSmart
Application Sheets

For a clear and precise diagnose

ISO 13485-2016
www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: cypress@diagnostics.be
Layout 20181024
 DIRECTIONS FOR USE

AUTOMATION

CYANSmart

CLINICAL CHEMISTRY

Version 20191127 - replaces all previous versions

 CY009 CYANSmart
Application sheet

Page Test Program Name

3 α-Amylase AAMY
4 Acid Phosphatase Total ACPt
5 Acid Phosphatase (Non) Prostatic ACP(n)p
6 Albumin ALB
7 Alkaline Phosphatase (ALP) ALP
8 Alkaline Phosphatase (ALP) Liquid ALPL
9 + 11-12 Bilirubin Direct + Scheme BILD
10 + 11-12 Bilirubin Total + Scheme BILT
13 Calcium CaAR
14 Chloride CL
15 Cholesterol Lyophilized CHOL
16 Cholesterol Liquid (new) CHOLLn
17 + 19 HDL Cholesterol Direct + Scheme HDLd
18 + 19 LDL Cholesterol Direct + Scheme LDLd
20 Creatine Kinase MB CKMB
21 Creatine Kinase MB Liquid CKMBL
22 Creatine Kinase NAC CKNAC
23 Creatine Kinase NAC Liquid CKNACL
24 Creatinine CRE
25 G6-PDH G6PDH
26 Glucose Lyophilized GLUC
27 Glucose Liquid GLUCL
28 Glucose Liquid NEW formulation GLUCLn
29 GT GGT
30 GT Liquid GGTL
31 GOT (AST) GOT
32 GOT (AST) Liquid GOTL
33 GPT (ALT) GPT
34 GPT (ALT) Liquid GPTL
35 HbA1c HbA1c
36 Hemoglobin HGB
37 Iron IRON
38 TIBC TIBC
39 LDH Liquid LDHL
40 Lipase LIPASE
41 Magnesium MgXB
42 Phosphorus PHOSPH
43 Potassium POT
44 Sodium SOD
45 Total Lipids TLIP
46 Total Protein TP
47 Total Protein in Urine and CSF TPU
48 Triglycerides TRIG
49 Triglycerides Liquid (new) TRIGLn
50 Urea UREA
51 Urea Liquid UREAL
52 Uric Acid Lyophilized UA
53 Uric Acid Liquid UAL
54 LIN340 LIN340
55 LIN510 LIN510
Legend:
1
Comments about Calibration
² Comments about Reagent blank
³ Comments about Normal ranges
4
Comments about Control serum ranges

Version 20191127 - replaces all previous versions 2

 CY009 CYANSmart
Application sheet

REF HBE03
α-Amylase
VOL 20 x 2 ml
Kinetic-colorimetric test
Standard - With CNPG3

REAGENT PREPARATION AND STABILITY

Reagent is ready to use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.

After opening the reagent is stable for 60 days when properly capped immediately after each opening and stored at 2°-8°C. The
reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample³ (Calibrator1)
(Calibrator1) 20 µl
Sample 20 µl³
Reagent 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: AAMY Blank Low: 0.00002

Program Method: Kinetic Blank High: 0.40002
Main Filter: 405 Normal Low: 28.0000 U/L
Sub Filter: None Normal High: 90.0000³ U/L
1
Program Unit: U/L Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1,3
Delay Time: 120 sec Factor: 3954.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
Dilution Factor: 1.0000 Control N Max: 0.00004 U/L
4
Linearity Min: 0.2439 U/L Control P Min: 0.0000 U/L
Linearity Max: 2200.0000 U/L Control P Max: 0.00004 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
3
These values are for serum or plasma samples. For urine samples; use 10 µl of sample, Normal High: 450 and Factor: 7908.
4
The control values can be found on the control sheets delivered together with the control vials.

LINEARITY
This method is linear up to 2200 U/l of α-amylase.
If the obtained results are greater than 2200 U/l, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

Version 20191127 - replaces all previous versions 3

 CY009 CYANSmart
Application sheet

REF HBE01
Acid Phosphatase Total
VOL 18 x 2 ml
Colorimetric kinetic test
Standard - α -Naphthyl phosphate. Hillmann

REAGENT PREPARATION AND STABILITY

R3 and R4 are ready to use.
Dissolve one tablet R2 substrate in 2 ml of R1 buffer. Cap and mix gently to dissolve all the contents. The stability of working
reagent is 2 days at 2-8ºC or 6 hours at room temperature. Do not use the tablets if they appear to be broken.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 100 µl
Sample 100 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: ACPt Blank Low: 0.00002

Program Method: Kinetic Blank High: 0.44002
Main Filter: 405 Normal Low: 0.1300 U/L
Sub Filter: None Normal High: 5.4000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 300 sec Factor: 750.000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 0.0000 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 150.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials.

LINEARITY
This method is linear up to 150 U/L of Acid Phosphatase.
If the obtained results are greater than 150 U/l, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

Version 20191127 - replaces all previous versions 4

 CY009 CYANSmart
Application sheet

REF HBE01
Acid Phosphatase (Non) Prostatic
VOL 18 x 2 ml
Colorimetric kinetic test
Standard - α-Naphthyl phosphate. Hillmann.

REAGENT PREPARATION AND STABILITY, CALIBRATION & QUALITY CONTROL & LINEARITY
see ACPt. (Page 4)

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
1
(Calibrator ) 100 µl
Sample 100 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Reagent 3 10 µl 10 µl 10 µl
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator).
Wait with the preparation of the next sample until the CYANSmart is finished with measuring the previous one. At this time, the
CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: ACPnp Blank Low: 0.00002

Program Method: Kinetic Blank High: 0.44002
Main Filter: 405 Normal Low: 0.1300 U/L
Sub Filter: None Normal High: 5.4000 U/L
1
Program Unit: U/L Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 300 sec Factor: 750.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 0.0000 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 150.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials.

NOTES
To obtain a value for Prostatic Acid Phosphatase (ACPp), you can calculate the ratio: ACPp = ACPt – ACPnp.

Version 20191127 - replaces all previous versions 5

 CY009 CYANSmart
Application sheet

REF HB0010 HB0010A HB0010M

Albumin
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Bromocresol Green
Standard 1 x 5 ml 4 x 5 ml - Colorimetric

REAGENT PREPARATION AND STABILITY

The reagent is ready to use.
All the components of the kit are stable at 2-25°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use. Once open, the standard is stable up to 3 months.
Do not exceed the temperature of 25°C during storage. The reagent should be a clear, yellow-green solution. The reagent should
be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 5 µl
Sample 5 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Then, incubate for 10 minutes at room temperature. After the incubation time,
aspirate and measure all the samples within one hour after preparation.

PROGRAM SETUP

Program Name: ALB Blank Low: 0.0000²

Program Method: End Point Blank High: 0.4000²
Main Filter: 620 Normal Low: 3.5000 g/dL
Sub Filter: None Normal High: 5.0000 g/dL
Program Unit: g/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: (value see vial) 1 g/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 g/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 g/dL
4
Linearity Min: 0.0380 g/dL Control P Min: 0.0000 g/dL
Linearity Max: 5.8000 g/dL Control P Max: 0.00004 g/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
4
The control values can be found on the control sheets delivered together with the control vials.

LINEARITY
This method is linear up to 5.8 g/dl of Albumin.
If the obtained results are greater than 5.8 g/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

Version 20191127 - replaces all previous versions 6

 CY009 CYANSmart
Application sheet

REF HBE02
Alkaline Phosphatase (ALP)
VOL 15 x 15 ml
Kinetic test optimized (DGKC)
Standard -

REAGENT PREPARATION AND STABILITY

Dissolve one tablet R2 in 15 ml of buffer Reagent R1. Cap and mix gently to dissolve the contents.
The stability of working reagent is 5 days at room temperature (15-25°C) or 21 days at 2-8°C. Do not use the tablets if they appear
to be broken.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 15 µl
Sample 15 µl
Working solution (R1 + R2) 0.9 ml 0.9 ml 0.9 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: ALP Blank Low: 0.00002

Program Method: Kinetic Blank High: 1.30002
Main Filter: 405 Normal Low: 98.0000 U/L
Sub Filter: None Normal High: 279.000 U/L
1
Program Unit: U/L Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 060 sec Factor: 3300.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 0.0000 U/L Control P Min: 0.00004 U/L
Linearity Max: 1200.0000 U/L Control P Max: 0.00004 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials.

LINEARITY
This method is linear up to 1200 U/l of Alkaline Phosphatase.
If the obtained results are greater than 1200 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

Version 20191127 - replaces all previous versions 7

 CY009 CYANSmart
Application sheet

REF HBE12
Alkaline Phosphatase Liquid
VOL 60 + 15 ml
Kinetic. Colorimetric. IFCC
Standard -

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Substrate).
The stability of the working reagent is 5 days at 15-25°C or 21 days at 2-8°C.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use. Do not freeze the reagents.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2.

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 20 µl
Sample 20 µl
Working solution (R1 + R2) 1 ml 1 ml 1 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: ALPL Blank Low: 0.00002

Program Method: Kinetic Blank High: 1.50002
Main Filter: 405 Normal Low: 26.0000 U/L
Sub Filter: None Normal High: 117.000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
Delay Time: 060 sec Factor: 2764.00001
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 1.3070 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 1400.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials.

LINEARITY
This method is linear up to 1400 U/l of Alkaline Phosphatase.
If the obtained results are greater than 1400 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

Version 20191127 - replaces all previous versions 8

 CY009 CYANSmart
Application sheet

REF HB0260 HB0020 HB0020A

Bilirubin Direct
VOL 2 x 125 ml 1 x 125 ml 4 x 125 ml
DMSO. Colorimetric
Standard - - -

REAGENT PREPARATION AND STABILITY

Reagents are ready to use
All the components of the kit are stable at 2-25°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use. The reagent should be discarded if turbidity or precipitation has
occurred, if color development has occurred in reagent N or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor 1, only when quality controls are within the defined ranges. Otherwise, use the Biochemistry
Calibrator (HBC03) for calibration. Use the value indicated on the insert ‘with sample blank’.1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature, protect samples from direct sunlight.
Then, pipette into a test tube:
Blank Sample Blank Sample Calibrator1 Blank Calibrator1
Calibrator1 100 µl 100 µl
Sample 100 µl 100 µl
Reagent N 25 µl 25 µl
Reagent D 750 µl 750 µl 750 µl 750 µl 750 µl
Thus, for every sample, you need to prepare 2 test tubes: one for measuring the sample blank (background coloration) and one for
measuring the real sample coloration. Add Reagent N only to the second tube. Add Reagent D last, mix and incubate for exactly 5
minutes at room temperature. Aspirate the mixture in the CYANSmart, exactly 5 minutes after addition of the Reagent D.
Use the pictures on page 11-12 as guidance.

PROGRAM SETUP
Program Name: BILD Blank Low: 0.00002
Program Method: End Point Blank High: 0.10002
Main Filter: 546 Normal Low: 0.0600 mg/dL
Sub Filter: None Normal High: 0.2500 mg/dL
1
Program Unit: mg/dL Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.00001 mg/dL
Delay Time: 001 sec Factor: 19.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 0.0600 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 20.0000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Serum² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
²The Blank method programmed is Serum. ! Pay attention, this determines the calculation of the results, not the blank aspiration!
1. Aspirate distilled water to adjust the instrument to zero (AD value)
2. “Test Blank”  “yes” and aspirate Reagent D (blank)
3. In the standard/sample menu,
a. First: “Aspirate Serum” = Standard/Sample Blank.
b. Secondly: “Aspirate Standard/Sample” = Standard/Sample (including 25 µl of RN).
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 20 mg/dl of Bilirubin Direct. If the obtained results are greater than 20 mg/dl, dilute the sample 1:2 with
saline solution, repeat the determination, and multiply the result by factor 2.

Version 20191127 - replaces all previous versions 9

 CY009 CYANSmart
Application sheet

REF HB0270 HB0020 HB0020A

Bilirubin Total
VOL 2 x 125 ml 1 x 125 ml 4 x 125 ml
DMSO. Colorimetric
Standard - - -

REAGENT PREPARATION AND STABILITY

Reagents are ready to use.
All the components of the kit are stable at 2-25°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred, if color development has occurred in reagent N or if the
blank absorbance is out of range²

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor 1, only when quality controls are within the defined ranges. Otherwise, use the Biochemistry
Calibrator (HBC03) for calibration. Use the value indicated on the insert ‘with sample blank’.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature, protect samples from direct sunlight. Then, pipette into a test tube:
Blank Sample Blank Sample Calibrator1 Blank Calibrator1
1 100 µl 100 µl
Calibrator
Sample 100 µl 100 µl
Reagent N 25 µl 25 µl
Reagent T 750 µl 750 µl 750 µl 750 µl 750 µl
Thus for every sample, you need to prepare 2 test tubes: one for measuring the sample blank (background coloration) and one for
measuring the real sample coloration. Add Reagent N only to the second tube. Add Reagent T last, mix and incubate for exactly 5
minutes at room temperature. Aspirate the mixture in the CYANSmart, exactly 5 minutes after addition of the Reagent T.
Use the pictures on page 11-12 as guidance.

PROGRAM SETUP

Program Name: BILT Blank Low: 0.00002

Program Method: End Point Blank High: 0.10002
Main Filter: 546 Normal Low: 0.2000 mg/dL
Sub Filter: None Normal High: 1.1000 mg/dL
Program Unit: mg/dL Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 mg/dL
Delay Time: 001 sec Factor: 12.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.0000 4 mg/dL
Linearity Min: 0.1000 mg/dL Control P Min: 0.00004 mg/dL
Linearity Max: 20.0000 mg/dL Control P Max: 0.0000 4 mg/dL
Blank: Serum² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² The Blank method programmed is Serum. ! Pay attention, this determines the calculation of the results, not the blank aspiration!
1. Aspirate distilled water to adjust the instrument to zero (AD value)
2. “Test Blank”  “yes” and aspirate Reagent T (blank)
3. In the standard/sample menu,
a. First: “Aspirate Serum” = Standard/Sample Blank.
b. Secondly: “Aspirate Standard/Sample” = Standard/Sample (including 25 µl of RN).
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 20 mg/dl of Bilirubin total. If the obtained results are greater than 20 mg/dl, dilute the sample 1:2 with
saline solution, repeat the determination, and multiply the result by factor 2.

Version 20191127 - replaces all previous versions 10

 CY009 CYANSmart
Application sheet

Version 20191127 - replaces all previous versions 11

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium • www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: cypress@diagnostics.be
 CY009 CYANSmart
Application sheet

Version 20191127 - replaces all previous versions 12

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium • www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: cypress@diagnostics.be
 CY009 CYANSmart
Application sheet

REF HB0030 HB0030A HB0030M

Calcium
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Arsenazo III. Colorimetric
Standard 1 x 5 ml 4 x 5 ml -

REAGENT PREPARATION AND STABILITY

The reagent is ready for use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 20 µl
Sample 20 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 2 minutes at 15-25°C. After the incubation time, aspirate
and measure all the samples within one hour after preparation.

PROGRAM SETUP

Program Name: CaAR Blank Low: 0.0000²

Program Method: End Point Blank High: 0.8000²
Main Filter: 620 Normal Low: 8.60003 mg/dL
3
Sub Filter: None Normal High: 10.2000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0800 µl CONC: 10.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
Linearity Min: 0.1629 mg/dL Control P Min: 0.00004 mg/dL
4
Linearity Max: 20.0000 mg/dL Control P Max: 0.0000 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 3
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 20 mg/dl of Calcium.
If the obtained results are greater than 20 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

Version 20191127 - replaces all previous versions 13

 CY009 CYANSmart
Application sheet

REF HB005
Chloride
VOL 2 x 125 ml
Thiocyanate. Colorimetric
Standard 1 x 5 ml

REAGENT PREPARATION AND STABILITY

The reagent is ready for use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit. 1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 37°C/15-25°C. After the incubation time,
aspirate and measure all the samples within 30 minutes after preparation.

PROGRAM SETUP

Program Name: Cl Blank Low: 0.0000²

Program Method: End Point Blank High: 0.1500²
Main Filter: 492 Normal Low: 95.00003 mmol/L
3
Sub Filter: None Normal High: 115.0000 mmol/L
Program Unit: mmol/L Num of STD: 1
Aspiration Volume: 0500 µl CONC: 125.00001 mmol/L
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mmol/L
Dilution Factor: 1.0000 Control N Max: 0.00004 mmol/L
Linearity Min: 0.4540 mmol/L Control P Min: 0.00004 mmol/L
4
Linearity Max: 190.0000 mmol/L Control P Max: 0.0000 mmol/L
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 190 mmol/l of Chloride.
If the obtained results are greater than 190 mmol/l, dilute the sample 1:2 with distilled water, repeat the determination, and
multiply the result by factor 2.

Version 20191127 - replaces all previous versions 14

 CY009 CYANSmart
Application sheet

REF HB006
Cholesterol Lyophilized
VOL 2 x 125 ml
Enzymatic. Colorimetric test (CHOD-POD)
Standard 1 x 5 ml

REAGENT PREPARATION AND STABILITY

Dissolve the contents of one bottle R2 Enzymes into the contents of one bottle R1 Buffer. Cap and mix gently to dissolve all the
contents. This working reagent is stable 4 months at 2–8°C or 40 days at room temperature when stored in a dark bottle.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit. 1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
1
Standard 10 µl
Sample 10 µl
Working reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate 15 minutes at 15-25°C or 10 min at 37°C. After the incubation
time, aspirate and measure all the samples within 45 minutes after preparation.

PROGRAM SETUP

Program Name: CHOL Blank Low: 0.00002

Program Method: End Point Blank High: 0.10002
Main Filter: 510 Normal Low: 120.0000 mg/dL
Sub Filter: None Normal High: 200.0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 200.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 0.00000 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 900.0000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 900 mg/dl of Cholesterol.
If the obtained results are greater than 900 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

Version 20191127 - replaces all previous versions 15

 CY009 CYANSmart
Application sheet

REF HBL010 HBL010A HBL010M

Cholesterol Liquid
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Enzymatic. Colorimetric test (CHOD-POD)
Standard 1 x 5 ml 4 x 5 ml -

REAGENT PREPARATION AND STABILITY

All the reagents are ready to use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit. 1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate 15 minutes at 15-25°C or 10 minutes at 37°C. After the
incubation time, aspirate and measure all the samples within 45 minutes after preparation.

PROGRAM SETUP

Program Name: CHOLLn Blank Low: 0.00002

Program Method: End Point Blank High: 0.26002
Main Filter: 510 Normal Low: 120.0000 mg/dL
Sub Filter: None Normal High: 200.0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 200.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
Linearity Min: 0.5210 mg/dL Control P Min: 0.00004 mg/dL
4
Linearity Max: 1000.0000 mg/dL Control P Max: 0.0000 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 1000 mg/dl of Cholesterol.
If the obtained results are greater than 1000 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and
multiply the result by factor 2.

Version 20191127 - replaces all previous versions 16

 CY009 CYANSmart
Application sheet

REF HBL011
HDL Cholesterol Direct
VOL 40 + 120 ml
Enzymatic Colorimetric
Standard -

REAGENT PREPARATION AND STABILITY

R1 and R2 are ready to use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified. The reagents are light sensitive. Do not
leave bottles open. Keep containers tightly closed and prevent contamination during their use. Do not freeze the reagents.
Once opened R1 and R2 are stable for 60 days at 2-8°C. The reagents should be a clear solution. If turbidity or precipitation has
occurred, the reagent should be discarded.
CALIBRATION & QUALITY CONTROL
Use the HDL/LDL calibrator (HBC11) for calibration. The concentration1 is lot specific and given on the label of the calibrator.
Control sera are recommended to monitor the performance of assay procedures. Use the HDL/LDL control set (HBC10). Prepare
and measure these controls the same as samples. If the control values4 are found outside the defined range, mentioned on the
labels of the control vials, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Reagent Blank² Calibrator Blank1 Calibrator1 Sample Blank Sample
1
Calibrator 10 µl 10 µl
Sample 10 µl 10 µl
Distilled water 10 µl
R1 750 µl 750 µl 750 µl 750 µl 750 µl
Mix and incubate for exactly 5 minutes at 37°C. And then add:
R2 250 µl - 250 µl - 250 µl
Mix and incubate for exactly 5 minutes at 37°C. And then aspirate.
Thus, for every sample, you need to prepare 2 test tubes: one for the Calibrator/Sample Blank, to measure the background
coloration caused by the sample, and one for the Calibrator/Sample to measure the coloration caused by the reaction. After mixing
Calibrator/Sample and R1, incubate at 37°C for exactly 5 minutes. Then add R2 only to the Calibrator/Sample tube, mix and
incubate for another 5 minutes at 37°C. Then aspirate the mixtures in the CYANSmart to measure exactly 10 minutes after adding
R1. You can prepare several samples simultaneously as long as you respect the times mentioned.
Use the illustrations p.19 for guidance to perform this test in a time-efficient way.
PROGRAM SETUP
Program Name: HDLd Blank Low: 0.00002
Program Method: End Point Blank High: 1.0000²
Main Filter: 578 Normal Low: 59.0000 mg/dL
Sub Filter: None Normal High: 80.0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: (value see vial)1 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 1.0600 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 184.8000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Serum² Cuvette Temp: 37 °C

Num of Blank: 1
1
Enter the concentration values shown on the calibrator vials (HBC11)
² The Blank method programmed is Serum! Pay attention, this determines the calculation of the results, not the blank aspiration!
1. Aspirate distilled water to adjust the instrument zero (AD value)
2. “Test Blank”  “YES” and aspirate the Reagent Blank (R1 + R2 + Water)
3. In the Calibrator/Sample menu:
a. “Aspirate Serum” = Calibrator/Sample Blank mixture (Calibrator/Sample + R1)
b. “Aspirate Standard/Sample” = Calibrator/sample mixture (Calibrator/Sample + R1 + R2)
4
The control values can be found on the labels of the HDL/LDL Direct control set vials (HBC10)
LINEARITY
This method is linear up to 184.8 mg/dl of HDL Cholesterol. If the obtained results are greater than 184.8 mg/dl, dilute the sample
1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

Version 20191127 - replaces all previous versions 17

 CY009 CYANSmart
Application sheet

REF HBL012
LDL Cholesterol Direct
VOL 40 + 120 ml
Enzymatic Colorimetric
Standard -

REAGENT PREPARATION AND STABILITY

R1 and R2 are ready to use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified. The reagents are light sensitive. Do not
leave bottles open. Keep containers tightly closed and prevent contamination during their use. Do not freeze the reagents.
Once opened R1 and R2 are stable for 60 days at 2-8°C. The reagents should be a clear solution. If turbidity or precipitation has
occurred, the reagent should be discarded.
CALIBRATION & QUALITY CONTROL
Use the HDL/LDL calibrator (HBC11) for calibration. The concentration1 is lot specific and given on the label of the calibrator.
Control sera are recommended to monitor the performance of assay procedures. Use the HDL/LDL control set (HBC10). Prepare
and measure these controls the same as samples. If the control values4 are found outside the defined range, mentioned on the
labels of the control vials, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Reagent Blank² Calibrator Blank1 Calibrator1 Sample Blank Sample
Calibrator 10 µl 10 µl
Sample 10 µl 10 µl
Distilled water 10 µl
R1 750 µl 750 µl 750 µl 750 µl 750 µl
Mix and incubate for exactly 5 minutes at 37°C. And then add:
R2 250 µl 250 µl 250 µl
Mix and incubate for exactly 5 minutes at 37°C. And then aspirate.
Thus for every sample, you need to prepare 2 test tubes: one for the Calibrator/Sample Blank, to measure the background
coloration caused by the sample, and one for the Calibrator/Sample to measure the coloration caused by the reaction. After mixing
Calibrator/Sample and R1, incubate at 37°C for exactly 5 minutes. Then add R2 only to the Calibrator/Sample tube, mix and
incubate for another 5 minutes at 37°C. Then aspirate the mixtures in the CYANSmart to measure exactly 10 minutes after adding
R1. You can prepare several samples simultaneously as long as you respect the times mentioned.
Use the illustrations p19 for guidance to perform this test in a time-efficient way.

PROGRAM SETUP
Program Name: LDLd Blank Low: 0.00002
Program Method: End Point Blank High: 1.0000²
Main Filter: 578 Normal Low: 50.00000 mg/dL
Sub Filter: None Normal High: 100.0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: (value see vial) 1 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
Linearity Min: 1.6400 mg/dL Control P Min: 0.00004 mg/dL
4
Linearity Max: 250.0000 mg/dL Control P Max: 0.0000 mg/dL
Blank: Serum² Cuvette Temp: 37 °C

Num of Blank: 1
1
Enter the concentration values shown on the calibrator vials (HBC11)
² The Blank method programmed is Serum! Pay attention, this determines the calculation of the results, not the blank aspiration!
1. Aspirate distilled water to adjust the instrument zero (AD value)
2. “Test Blank”  “YES” and aspirate the Reagent Blank (R1 + R2 + Water)
3. In the Calibrator/Sample menu:
a. “Aspirate Serum” = Calibrator/Sample Blank mixture (Calibrator/Sample + R1)
b. “Aspirate Standard/Sample” = Calibrator/sample mixture (Calibrator/Sample + R1 + R2)
4 The control values can be found on the labels of the HDL/LDL Direct control set vials (HBC10)

LINEARITY
This method is linear up to 250.0 mg/dl of LDL Cholesterol. If the obtained results are greater than 250.0 mg/dl, dilute the sample
1:2 with saline solution, repeat the determination, and multiply the result by factor 2.

Version 20191127 - replaces all previous versions 18

 CY009 CYANSmart
Application sheet

Version 20191127 - replaces all previous versions 19

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium • www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: cypress@diagnostics.be
 CY009 CYANSmart
Application sheet

REF HBE10
Creatine Kinase MB
VOL 19 x 2,5 ml
Immunoinhibition. Kinetic UV
CK control 1 x 2 ml

REAGENT PREPARATION AND STABILITY

Dissolve one tablet R2 in 2,5 ml buffer R1.
The working reagent is stable for 8 days at 2-8ºC or 24 hours at room temperature (15-25°C). Do not use the tablets if they appear
to be broken.
Control: Dissolve the contents in 2 ml of distilled water. Cap vial and mix gently to dissolve the contents.
Stability: 8 hours at room temperature, 5 days at 2-8°C or 1 month at –20°C. Bring at room temperature for 30 min before use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor.
Control sera are recommended to monitor the performance of assay procedures. Use the CK control (HBC08) included in the kit.
Prepare and measure these controls the same as samples. If the control values are found outside the defined range, mentioned on
the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank1 Sample (Calibrator)
1
(Calibrator ) 40 µl
Sample 40 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: CKMB Blank Low: 0.00002

Program Method: Kinetic Blank High: 1.60002
Main Filter: 340 Normal Low: 2.8500 U/L
Sub Filter: None Normal High: 24.0000 U/L
Program Unit: U/L Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.0000 U/L
Delay Time: 180 sec Factor: 8255.0000
Test Time: 120 sec Control N Min: 0.00004 U/L
Dilution Factor: 1.0000 Control N Max: 0.00004 U/L
Linearity Min: 3.1100 U/L Control P Min: 0.0000 4 U/L
Linearity Max: 1000.0000 U/L Control P Max: 0.00004 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
2
Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 1000 U/l of CK-NAC (CK-MB is between 6 and 25% of total CK activity).
If the obtained results are greater than 1000 U/l for CK-NAC, dilute the sample 1:2 with saline solution, repeat the determination,
and multiply the result by factor 2.

Version 20191127 - replaces all previous versions 20

 CY009 CYANSmart
Application sheet

REF HBEL05
Creatine Kinase MB Liquid
VOL 60 + 15 ml
Immunoinhibition. Kinetic UV
CK control 1 x 2 ml

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of Reagent 1 with 1 volume of Reagent 2.
The stability of the working reagent is 7 days at 2-8°C or 12 hours at room temperature (15-25°C).
Control: Dissolve the contents in 2 ml of distilled water. Cap vial and mix gently to dissolve the contents.
Stability: 8 hours at room temperature, 5 days at 2-8°C or 1 month at –20°C. Bring at room temperature for 30 min before use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor.
Control sera are recommended to monitor the performance of assay procedures. Use the CK control (HBC08) included in the kit.
Prepare and measure these controls the same as samples. If the control values are found outside the defined range, mentioned on
the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank1 Sample (Calibrator)
1
(Calibrator ) 40 µl
Sample 40 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: CKMBL Blank Low: 0.00002

Program Method: Kinetic Blank High: 1.20002
Main Filter: 340 Normal Low: 1.0000 U/L
Sub Filter: None Normal High: 24.0000 U/L
Program Unit: U/L Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.0000 U/L
Delay Time: 180 sec Factor: 8255.0000
Test Time: 120 sec Control N Min: 0.00004 U/L
Dilution Factor: 1.0000 Control N Max: 0.00004 U/L
Linearity Min: 1.9000 U/L Control P Min: 0.0000 4 U/L
Linearity Max: 318.0000 U/L Control P Max: 0.0000 4 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1

2
Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 318 U/l of CK-MB.
If the obtained results are greater than 318 U/l, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HBE04
Creatine Kinase NAC
VOL 20 x 2,5 ml
Kinetic UV. NAC Activated
Standard -

REAGENT PREPARATION AND STABILITY

Dissolve one tablet R2 in 2,5 ml of buffer Reagent R1.
The stability of working reagent is 10 days at 2-8°C or 3 days at room temperature (15-25°C). Do not use the tablets if they appear
to be broken.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02) or the CK control (HBC08). Prepare and measure these controls the same as samples. If the control
values are found outside the defined range, mentioned on the insert of the control, check the instrument, reagents and calibration
for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 20 µl
Sample 20 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: CKNAC Blank Low: 0.00002

Program Method: Kinetic Blank High: 1.00002
Main Filter: 340 Normal Low: 34.0000 U/L
Sub Filter: None Normal High: 195.0000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
Delay Time: 120 sec Factor: 8095.00001
Test Time: 090 sec Control N Min: 0.00004 U/L
Dilution Factor: 1.0000 Control N Max: 0.00004 U/L
4
Linearity Min: 0.8780 U/L Control P Min: 0.0000 U/L
Linearity Max: 1300.0000 U/L Control P Max: 0.00004 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 1300 U/l of CK-NAC.
If the obtained results are greater than 1300 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HBEL03
Creatine Kinase NAC Liquid
VOL 60 + 15 ml
Kinetic UV. NAC Activated
Standard -

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of reagent R1 with 1 volume of reagent R2.
The stability of the working reagent is 2 weeks at 2-8°C or 48 hours at room temperature (15-25°C).
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02) or the CK control (HBC08). Prepare and measure these controls the same as samples. If the control
values are found outside the defined range, mentioned on the insert of the control, check the instrument, reagents and calibration
for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 20 µl
Sample 20 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: CKNACL Blank Low: 0.00002

Program Method: Kinetic Blank High: 1.00002
Main Filter: 340 Normal Low: 34.0000 U/L
Sub Filter: None Normal High: 195.0000 U/L
1
Program Unit: U/L Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 120 sec Factor: 8095.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 2.1200 U/L Control P Min: 0.00004 U/L
Linearity Max: 2000.0000 U/L Control P Max: 0.00004 U/L
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 2000 U/l of CK-NAC.
If the obtained results are greater than 2000 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HB0080 HB0080A HB0080M

Creatinine
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Jaffé
Standard 1 x 5 ml 4 x 5 ml -

REAGENT PREPARATION AND STABILITY

Reagent mixture: Mix proportionally 1:1 the reagents R1 and R2.
The working reagent is stable for 10 days at 15-25°C.
All the components of the kit are stable at 2-25°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample Standard1
Standard1 100 µl
Sample 100 µl
Working reagent (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: CRE Blank Low: 0.00002

Program Method: Two Point Blank High: 1.80002
Main Filter: 510 Normal Low: 0.60003 mg/dL
Sub Filter: None Normal High: 1.40003 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 2.00001 mg/dL
Delay Time: 030 sec Factor: 0.0000
Test Time: 060 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 0.1150 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 15.00000 mg/dL Control P Max: 0.00004 mg/dL
2
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 15 mg/dl of Creatinine.
If the obtained results are greater than 15 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HBE11
G6-PDH
VOL 100 + 20 ml
U.V. Enzymatic
Standard -

REAGENT PREPARATION AND STABILITY

R1 and R4 are ready to use.
Reconstitute the content of R2 and R3 with 2 ml of distilled water each.
After opening, R1 and R4 stable until the expiration date specified on the label at 2-8°C and the reconstituted R2 and R3 are stable
for 4 weeks at 2-8ºC, when stored tightly closed, protected from light and contaminations are prevented during their use.

CALIBRATION & QUALITY CONTROL

Calibration by means of a factor.
Control sera are recommended to monitor the performance of assay procedures. Use the 2 levels of G6-PDH controls (HBC09).
Prepare and measure these controls the same as samples. If the control values are found outside the defined range, mentioned on
the control vial, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
1. PREPARATION for ERYTHROCYTES
Wash 0,2 ml of blood with 2 ml of 0.9% NaCl (saline) solution. Centrifuge after each wash for 10 min at around 3000 rpm. Repeat 3
times. Suspend the washed and centrifuged erythrocytes in 0,5 ml of R4 and let stand for 15 min at +4°C and then centrifuge again.
Use the supernatant in the assay within 2 hours.
2. TEST STEP for ERYTHROCYTES
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Sample
Hemolysate 15 µl
Reagent 1 1.0 ml
Reagent 2 30 µl
Mix and incubate for 5 min at 37°C, then add:
Reagent 3 15 µl
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample. Wait with the preparation of the next sample until the CYANSmart is finished with measuring the
previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: G6PDH Blank Low: 0.0000

Program Method: Kinetic Blank High: 0.0100
Main Filter: 340 Normal Low: 1170.00003 U/L
Sub Filter: None Normal High: 2455.00003 U/L
Program Unit: U/L Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.0000 U/L
Delay Time: 003 sec Factor: 33650.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
Dilution Factor: 1.0000 Control N Max: 0.0000 4 U/L
Linearity Min: 154.0000 U/L Control P Min: 0.0000 4 U/L
Linearity Max: 4303.0000 U/L Control P Max: 0.0000 4 U/L
Blank: Water Cuvette Temp: 37 °C

Num of Blank: 1
3
These values are for erythrocytes (U/L). To obtain results in U/g HGB, divide the results of this analysis with the results for
Hemoglobin (g/L) for that sample.
4
The control values can be found on the control vials

LINEARITY
This method is linear up to 4303 U/L of G6-PDH.
If the obtained results are greater than 4303 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and
multiply the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HB009
Glucose Lyophilized
VOL 2 x 125 ml
Enzym Enzymatic. Colorimetric
Standard - GOD-POD

REAGENT PREPARATION AND STABILITY

Dissolve the contents of one bottle R2 Enzymes in one bottle R1 buffer. This working reagent is stable 1 month after constitution
at 2-8°C or 7 days at room temperature (15-25°C).
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit. 1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Blank Sample Standard1
1
Standard 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 min at 37°C or 20 minutes at 15-25°C.
After the incubation time, aspirate and measure all the samples within 30 minutes.

PROGRAM SETUP

Program Name: GLUC Blank Low: 0.00002

Program Method: End Point Blank High: 0.10002
Main Filter: 510 Normal Low: 60.0000³ mg/dL
Sub Filter: None Normal High: 110.0000³ mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 100.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.0000 4 mg/dL
Linearity Min: 0.0000 mg/dL Control P Min: 0.00004 mg/dL
Linearity Max: 500.0000 mg/dL Control P Max: 0.0000 4 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For CSF samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 500 mg/dl of Glucose.
If the obtained results are greater than 500 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

Old formulation until lot GL-00721/3, newer lots: see GLUCLn

REF HBL04 HBL04A
Glucose Liquid
VOL 2 x 125 ml 8 x 125 ml
Enzym Enzymatic. Colorimetric
Standard 1 x 5 ml 4 x 5 ml GOD-POD

REAGENT PREPARATION AND STABILITY

The reagent is ready for use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit. 1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
1
Standard 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 15 min at 37°C. After the incubation time, aspirate and
measure all the samples within 45 minutes.

PROGRAM SETUP
Program Name: GLUCL Blank Low: 0.00002
Program Method: End Point Blank High: 0.32002
Main Filter: 510 Normal Low: 60.0000³ mg/dL
Sub Filter: None Normal High: 110.0000³ mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 100.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.0000 4 mg/dL
Linearity Min: 1.1800 mg/dL Control P Min: 0.00004 mg/dL
Linearity Max: 600.0000 mg/dL Control P Max: 0.0000 4 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For CSF samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 600 mg/dl of Glucose.
If the obtained results are greater than 600 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HBL04 HBL04A HBL04M New formulation starting from lot GL-00731
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Glucose Liquid
Standard 1 x 5 ml 4 x 5 ml -
Enzymatic. Colorimetric. GOD-POD

REAGENT PREPARATION AND STABILITY

The reagent is ready for use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit. 1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 min at 37°C or 15 min at 15-25°C.
After the incubation time, aspirate and measure all the samples within 40 minutes.

PROGRAM SETUP

Program Name: GLUCLn Blank Low: 0.00002

Program Method: End Point Blank High: 0.32002
Main Filter: 510 Normal Low: 74.0000 mg/dL
Sub Filter: None Normal High: 100.0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 100.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.0000 4 mg/dL
Linearity Min: 3.2200 mg/dL Control P Min: 0.00004 mg/dL
Linearity Max: 460.0000 mg/dL Control P Max: 0.0000 4 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 460 mg/dl of Glucose.
If the obtained results are greater than 460 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HBE05
γ-GT
VOL 15 x 15 ml
(Gamma-Glutamyl-Transferase)
Standard - Kinetic test. Carboxy substrate

REAGENT PREPARATION AND STABILITY

Dissolve one tablet of R2 Substrate into one 15ml R1 Buffer. Cap and mix gently to dissolve all the contents.
This working reagent is stable 21 days at 2-8°C or 5 days at room temperature. Do not use the tablets if they appear to be broken.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 100 µl
Sample 100 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: GGT Blank Low: 0.00002

Program Method: Kinetic Blank High: 1.80002
Main Filter: 405 Normal Low: 7.0000 U/L
Sub Filter: None Normal High: 50.0000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 060 sec Factor: 1190.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 0.0000 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 375.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent2 Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 375 U/L of γ-GT.
If the obtained results are greater than 375 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HBEL06 HBEL061

γ-GT Liquid
VOL 240 + 60 ml 60 + 15 ml
(Gamma-Glutamyl-Transferase)
Standard - - Kinetic test. Carboxy substrate

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Substrate). ).
The stability of this working reagent is 21 days at 2-8°C or 5 days at room temperature (15-25°C).
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 100 µl
Sample 100 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: GGTL Blank Low: 0.00002

Program Method: Kinetic Blank High: 1.80002
Main Filter: 405 Normal Low: 7.0000 U/L
Sub Filter: None Normal High: 50.0000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 060 sec Factor: 1190.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 2.0000 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 300.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent2 Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 300 U/L of γ-GT.
If the obtained results are greater than 300 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HBE06
GOT (AST)
VOL 15 x 15 ml
U.V. Kinetic test
Standard - According IFCC

REAGENT PREPARATION AND STABILITY

Dissolve one tablet of R2 Substrate in 15 ml of R1 Buffer. Cap and mix gently to dissolve all the contents.
This working reagent is stable 72 hours at 15-25°C or 21 days at 2-8°C. Do not use the tablets if they appear to be broken.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.

The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 100 µl
Sample 100 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: GOT Blank Low: 1.00002

Program Method: Kinetic Blank High: 2.50002
Main Filter: 340 Normal Low: 0.0000 U/L
Sub Filter: None Normal High: 38.0000 U/L
1
Program Unit: U/L Num of STD: 0
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 060 sec Factor: 1750.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
Dilution Factor: 1.0000 Control N Max: 0.00004 U/L
4
Linearity Min: 0.0000 U/L Control P Min: 0.0000 U/L
Linearity Max: 360.0000 U/L Control P Max: 0.00004 U/L
2
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 360 U/L of GOT.
If the obtained results are greater than 360 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HBEL010 HBEL010A HBEL010M

GOT (AST) Liquid
VOL 240 + 60 ml 6x120+3x60 ml 6x30+3x15 ml
NADH. U.V. Kinetic
Standard - - - According to IFCC

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Substrate).
The stability of the working reagent is 24 hours at 15-25°C or 14 days at 2-8°C.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
1
(Calibrator ) 100 µl
Sample 100 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: GOTL Blank Low: 1.00002

Program Method: Kinetic Blank High: 2.50002
Main Filter: 340 Normal Low: 3.1200 U/L
Sub Filter: None Normal High: 38.0000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
Delay Time: 060 sec Factor: 1750.00001
Test Time: 090 sec Control N Min: 0.00004 U/L
Dilution Factor: 1.0000 Control N Max: 0.00004 U/L
Linearity Min: 3.1200 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 260.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent2 Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control vials

LINEARITY
This method is linear up to 260 U/L of GOT.
If the obtained results are greater than 260 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HBE07
GPT (ALT)
VOL 15 x 15 ml
U.V. Kinetic test
Standard - According to IFCC

REAGENT PREPARATION AND STABILITY

Dissolve one tablet of R2 Substrate in 15 ml of R1 Buffer. Cap and mix gently to dissolve all the contents.
This working reagent is stable 72 hours at 15-25°C or 21 days at 2-8°C. Do not use the tablets if they appear to be broken.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 100 µl
Sample 100 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: GPT Blank Low: 1.00002
Program Method: Kinetic Blank High: 2.50002
Main Filter: 340 Normal Low: 0.0000 U/L
Sub Filter: None Normal High: 40.0000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 060 sec Factor: 1750.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 0.0000 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 400.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent2 Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 400 U/L of GPT.
If the obtained results are greater than 400 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HBEL020 HBEL020A HBEL020M

GPT (ALT) Liquid
VOL 240 + 60 ml 6x120+3x60 ml 6x30+3x15 ml
NADH. U.V. Kinetic
Standard 1 x 5 ml 4 x 5 ml - According to IFCC

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Substrate).
The stability of the working reagent is 24 hours at 15-25°C or 4 weeks at 2-8°C.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
1
(Calibrator ) 100 µl
Sample 100 µl
Working solution (R1 + R2) 1.0 ml 1.0 ml 1.0 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: GPTL Blank Low: 1.00002

Program Method: Kinetic Blank High: 2.50002
Main Filter: 340 Normal Low: 3.0000 U/L
Sub Filter: None Normal High: 40.0000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
Delay Time: 060 sec Factor: 1750.00001
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 3.0000 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 260.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent2 Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 260 U/L of GPT.
If the obtained results are greater than 260 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and multiply
the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HT001
HbA c1
VOL 30 x 10 ml
Turbidimetric
Standard -

REAGENT PREPARATION AND STABILITY

Reagent R1, R2 and R3 are ready to use.
Reagent 1 and 2 are stable for at least one month after opening stored at 2-8°C.
All the components of the kit are stable until the expiration date on the label when stored tightly closed at 2-8ºC and
contaminations are prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred.

CALIBRATION & QUALITY CONTROL

Use the HbA1c Calibrator set (HT001S) Program the values mentioned on the vials in the method programming. Use saline
solution (9 g/L NaCl) or distilled water as STD 1, with value 0.001.
Control sera are recommended to monitor the performance of assay procedures. Use the HbA1c-control set (HT001C). Prepare
and measure these controls the same as samples. If the control values are found outside the defined range, mentioned on the
control vial, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
1. Precipitation
To determine HbA1c, a hemolysate must be prepared for each sample as well as for Calibrator HT001S (Level 1 to 4) and Control
HT001C:
- Dispense 1 mL of Reagent 3 into labeled test tubes (glass or plastic). Also provide test tubes for Calibrator and Control.
- Add 20 μL of well mixed whole blood (sample, calibrator, control). Mix.
- Allow to stand for 5 minutes or until complete lysis is evident. Hemolysates may be stored up to 10 days at 2-8°C.
2. Test step
Make sure the samples and reagents are at room temperature. Then, pipette into a test tube:
Blank Sample Calibrators
Calibrators (hemolysed) 15 µl
Sample (hemolysed) 15 µl
Reagent 1 540 µl 540 µl 540 µl
Mix and incubate for exactly 5 min at 37°C. And then add:
Reagent 2 180 µl 180 µl 180 µl
Mix and incubate for exactly 5 min at 37°C. And then aspirate:
You can prepare several samples simultaneously, as long as you respect the times mentioned!
PROGRAM SETUP
Program Name: HbA1c Normal Low: 2.0000 %
Program Method: End Point Normal High: 6.0000 %
Main Filter: 620 Num of STD: 5
Sub Filter: None STD 1 CONC: 0.0010 %
Program Unit: % STD 2 CONC: * (=CAL 1) %
Aspiration Volume: 0500 µl STD 3 CONC: * (=CAL 2) %
Delay Time: 001 sec STD 4 CONC: * (=CAL 3) %
Test Time: 003 sec STD 5 CONC: * (=CAL 4) %
Dilution Factor: 1.0000 Factor: 0.0000
Linearity Min: 2.0000 % Control N Min: 0.00004 %
Linearity Max: 16.0000 % Control N Max: 0.00004 %
4
Blank: Reagent Control P Min: 0.0000 %

Num of Blank: 1 Control P Max: 0.00004 %

0.0000 37 °C
Blank Low: Cuvette Temp:
Blank High: 2.0000
*: enter the concentration values shown on the calibrator vials. STD1 corresponds with a saline solution of 9 g/L or distilled water, STD 2
corresponds to calibrator 1; STD 3 to calibrator 2; STD 4 to calibrator 3 and STD 5 to calibrator 4.
4
The control values can be found on the control vial
LINEARITY
This method is linear up to 16% of HbA1c.

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 CY009 CYANSmart
Application sheet

REF HB011
Hemoglobin
VOL 4 x 5 ml
Drabkin
Standard - Colorimetric test

REAGENT PREPARATION AND STABILITY

Working reagent: 4,9 ml distilled water + 2 drops of reagent, or 245 ml distilled water + 5 ml of reagent and mix.
The working reagent is stable 2 months at 2 –8°C, protected from the sunlight.

All the components of the kit and the hemoglobin calibrator (HBS02) are stable at 2-8°C up to the date of expiration as specified,
when stored tightly closed, protected from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Use the Hemoglobin calibrator (HBS02).
Control sera are recommended to monitor the performance of assay procedures. If control values are found outside the defined
range, check the instrument, reagents and calibrator for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Calibrator1
Calibrator1 10 µl
Sample 10 µl
Working reagent 2.5 ml 2.5 ml 2.5 ml
Mix and incubate for 3 minutes at room temperature 15-25°C, then aspirate to measure. You can prepare several samples
simultaneously, as long as you respect the times mentioned!

PROGRAM SETUP

Program Name: HGB Blank Low: 0.0000²

Program Method: End Point Blank High: 0.0100²
Main Filter: 546 Normal Low: 12.0000 g/dL
Sub Filter: None Normal High: 18.0000 g/dL
Program Unit: g/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 15.0000 g/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 g/dL
4
Dilution Factor: 1.0000 Control N Max: 0.0000 g/dL
Linearity Min: 0.1000 g/dL Control P Min: 0.00004 g/dL
4
Linearity Max: 20.0000 g/dL Control P Max: 0.0000 g/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 20 g/dl of Hemoglobin.
If the obtained results are greater than 20 g/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB012
Iron
VOL 4 x 50 ml
Colorimetric test. ‘‘Ferrozine’’
Standard 1 x 10 ml

REAGENT PREPARATION AND STABILITY

R3 is ready to use.
Add the contents of one tube R2 reductant to the contents of one bottle R1 buffer. Cap and mix gently to dissolve all the contents.
This working reagent is stable for 3 months at 2-8°C or 1 month at room temperature (15-25°C).
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.
CALIBRATION & QUALITY CONTROL
Use the standard included in the kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Reagent Blank Sample Blank Sample Standard Blank1 Standard1
Standard 200 µl 200 µl
Sample 200 µl 200 µl
Distilled Water 200 µL
Working reagent (R1+R2) 1.0 ml 1.0 ml 1.0 ml 1.0 ml 1.0 ml
Reagent 3 1 drop 1 drop 1 drop
Mix and incubate for 10 minutes at 15-25°C. After the incubation time, aspirate and measure all the samples within 30 min after
preparation.
Thus, for every sample, you need to prepare 2 test tubes: one for measuring the sample blank (background coloration) and one for
measuring the real sample coloration.
PROGRAM SETUP

Program Name: IRON Blank Low: 0.00002

Program Method: End Point Blank High: 0.10002
Main Filter: 578 Normal Low: 40.0000 µg/dL
Sub Filter: None Normal High: 175.0000 µg/dL
Program Unit: µg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 100.00001 µg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 µg/dL
Dilution Factor: 1.0000 Control N Max: 0.0000 4 µg/dL
Linearity Min: 0.85000 µg/dL Control P Min: 0.0000 4 µg/dL
Linearity Max: 1000.0000 µg/dL Control P Max: 0.00004 µg/dL
2 °C
Blank: Serum Cuvette Temp: 37
Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
2
The Blank method programmed is Serum. ! Pay attention, this determines the calculation of the results, not the blank aspiration!
- Aspirate distilled water to adjust the instrument to zero (AD value)
- “Test Blank”  “yes” and aspirate the Reagent Blank
- In the standard/sample menu,
a. First: “Aspirate Serum” = Standard/Sample Blank.
b. Secondly: “Aspirate Standard/Sample” = Standard/Sample (including 1 drop of R3).
4
The control values can be found on the control sheets delivered together with the control
LINEARITY
This method is linear up to 1000 µg/dl of Iron. If the obtained results are greater than 1000 µg/dl, dilute the sample 1:2 with saline
solution, repeat the determination, and multiply the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB017
TIBC
VOL 100 tests
(Total Iron Binding Capacity).
Standard - Saturation and precipitating reagents.

REAGENT PREPARATION AND STABILITY

The reagents are ready to use.
See also Iron determination, code HB012.
CALIBRATION & QUALITY CONTROL
Use the standard included in the iron kit. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.
SAMPLE PREPARATION
1. PRECIPITATION
Do NOT precipitate the standard! Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Sample
Sample 500 µl
Reagent 1 1.0 ml
Mix and incubate for 10 min at 15-25°C, then add:
Reagent 2 3 dosage (spoonful)
Mix and incubate 10 min at 15-25°C, then centrifuge 15 min at 3000 rpm. Collect the supernatant and prepare for every sample 2
test tubes to perform the test step. (See Iron).
2. TEST STEP
Reagent Blank Sample Blank Sample Standard Blank1 Standard1
Standard 200 µl 200 µl
Sample 200 µl 200 µl
Distilled Water 200 µL
Working reagent (R1+R2) 1.0 ml 1.0 ml 1.0 ml 1.0 ml 1.0 ml
Reagent 3 1 drop 1 drop 1 drop
Mix and incubate for 10 minutes at 15-25°C. After the incubation time, aspirate and measure all the samples within 30 min after
preparation.
PROGRAM SETUP
Program Name: TIBC Blank Low: 0.0000
Program Method: End Point Blank High: 0.1000
Main Filter: 578 Normal Low: 200.0000 µg/dL
Sub Filter: None Normal High: 400.0000 µg/dL
Program Unit: µg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 100.0000 µg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 µg/dL
4
Dilution Factor: 3.0000 Control N Max: 0.0000 µg/dL
Linearity Min: 3.0000 µg/dL Control P Min: 0.00004 µg/dL
4
Linearity Max: 1000.0000 µg/dL Control P Max: 0.0000 µg/dL
Blank: Serum2 Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned on the insert
provided with HBC03.
2
The Blank method programmed is Serum. ! Pay attention, this determines the calculation of the results, not the blank aspiration!
- Aspirate distilled water to adjust the instrument to zero (AD value)
- “Test Blank”  “yes” and aspirate Reagent Blank mixture
- In the standard/sample menu,
a. First: “Aspirate Serum” = Standard/Sample Blank.
b. Secondly: “Aspirate Standard/Sample” = Standard/Sample (including 1 drop of R3).
4
The control values can be found on the control sheets delivered together with the control
LINEARITY
This method is linear up to 1000 µg/dl of Iron. If the obtained results are greater than 1000 µg/dl, dilute the sample 1:2 with saline
solution, repeat the determination, and multiply the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HBEL04 HBEL041

LDH Liquid
VOL 240 + 60 ml 60 + 15 ml
Lactate Dehydrogenase
Standard - - Kinetic test UV. DGKC

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Substrate).
The stability of working reagent is 21 days at 2-8°C or 15 days at room temperature (15-25°C).
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range².

CALIBRATION & QUALITY CONTROL

Calibration by means of a Factor1. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample (Calibrator1)
(Calibrator1) 15 µl
Sample 15 µl
Working solution (R1 + R2) 0.9 ml 0.9 ml 0.9 ml
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP

Program Name: LDHL Blank Low: 1.00002

Program Method: Kinetic Blank High: 2.50002
Main Filter: 340 Normal Low: 230.0000 U/L
Sub Filter: None Normal High: 460.0000 U/L
Program Unit: U/L Num of STD: 01
Aspiration Volume: 0500 µl CONC: 0.00001 U/L
1
Delay Time: 060 sec Factor: 9690.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 3.4200 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 1600.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent2 Cuvette Temp: 37 °C

Num of Blank: 1
1
Calibration by means of a Factor. Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of
STD: 1 and CONC: as mentioned on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 1600 U/L of LDH.
If the obtained results are greater than 1600 U/l, dilute the sample 1:10 with saline solution, repeat the determination, and
multiply the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HBE09
Lipase
VOL 4 x 10 ml
Enzymatic. Colorimetric.
Calibrator 1 vial (Lyo.)

REAGENT PREPARATION AND STABILITY

Reagent 1 & 2: Ready to use. Stability after opening 90 days at 2-8°C.
R2: Mix gently before use (note 1 on insert).

Calibrator: Reconstitute the contents of one vial with 1 mL of distilled water. Mix gently until complete solution.
Stability: 7 days at 2-8ºC. Divide standard solution into small volumes and freeze. Stability: 3 months at -20ºC.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
R1 should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². R2 is a turbid orange-
colored micro-emulsion, discard if turning red.

CALIBRATION & QUALITY CONTROL

Use the calibrator included in the kit1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank² Sample Calibrator1
Distilled water 10 µl
Calibrator1 10 µl
Sample 10 µl
Reagent 1 1.0 ml 1.0 ml 1.0 ml
Reagent 2 200 µl 200 µl 200 µl
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”.

PROGRAM SETUP
Program Name: LIPASE Blank Low: 0.00002
Program Method: Kinetic Blank High: 1.40002
Main Filter: 578 Normal Low: 5.0000 U/L
Sub Filter: None Normal High: 38.0000 U/L
1
Program Unit: U/L Num of STD: 1
Aspiration Volume: 0500 µl CONC: (value see insert)1 U/L
1
Delay Time: 060 sec Factor: 0.0000
Test Time: 090 sec Control N Min: 0.00004 U/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 U/L
Linearity Min: 5.0000 U/L Control P Min: 0.00004 U/L
4
Linearity Max: 250.0000 U/L Control P Max: 0.0000 U/L
Blank: Reagent2 Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively,
you can program and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 250 U/L of Lipase. If the obtained results are greater than 250 U/l, dilute the sample 1:10 with saline
solution, repeat the determination, and multiply the result by factor 10.

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 CY009 CYANSmart
Application sheet

REF HBE320 HBE320A HBE320M

Magnesium
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Xylidyl Blue-EGTA. Colorimetric
Standard 1 x 5 ml 4 x 5 ml -

REAGENT PREPARATION AND STABILITY

The reagent is ready for use
All the components of the kit are stable at 2-25°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit. 1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagent and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at 15-25° or 3 min at 37°C. After the incubation
time, aspirate and measure all the samples within 45 min after preparation.

PROGRAM SETUP

Program Name: MgXB Blank Low: 0.00002

Program Method: End Point Blank High: 1.50002
Main Filter: 510 Normal Low: 1.6000³ mg/dL
Sub Filter: None Normal High: 2.5000³ mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 2.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
Linearity Min: 0.0500 mg/dL Control P Min: 0.00004 mg/dL
4
Linearity Max: 6.6000 mg/dL Control P Max: 0.0000 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 3
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 6.6 mg/dl of Magnesium.
If the obtained results are greater than 5 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB014
Phosphorus
VOL 2 x 125 ml
U.V.-test. Phosphmolybdate
Standard 1 x 5 ml

REAGENT PREPARATION AND STABILITY

The reagent is ready for use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

AMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
1
Standard 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
Mix and incubate for 5 minutes at 37°C. After the incubation time, aspirate and measure the sample.
You can prepare several samples simultaneously, as long as you respect the time mentioned!

PROGRAM SETUP

Program Name: PHOSPH Blank Low: 0.00002

Program Method: End Point Blank High: 0.54002
Main Filter: 340 Normal Low: 2.5000³ mg/dL
Sub Filter: None Normal High: 5.0000³ mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 5.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 0.0000 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 35.0000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 35 mg/dl of Phosphorus.
If the obtained results are greater than 35 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB015
Potassium
VOL 3 x 50 ml
Colorimetric test. NaTPB
Standard 1 x 3 mL

REAGENT PREPARATION AND STABILITY

Shake Reagent 2 (NaOH) before use.
Mix proportionally 1:1 Reagent 1 and Reagent 2. After mixing, incubate for 30 minutes prior to use. Before each use, the working
reagent must be shaken. The working reagent is stable for 7 days at 15-25°C and 30 days at 2-8°C.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use. Handle standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Sample
Sample 50 µl
Reagent 3 500 µl
Mix carefully. Centrifuge for 5-10 min at 5000 rpm. Collect the supernatant in a new test tube:
Blank Sample Standard1
Standard1 100 µl
Supernatant 100 µl
Working Reagent (R1+R2) 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. To produce a homogeneous turbidity, the standard or the clear supernatant
must be added to the center of the surface of the working reagent in the test tube. Mix each test tube carefully before proceeding
to the next sample. Mix and allow for stand for 5 min. After the incubation time, aspirate and measure all the samples within 30
min after addition of the working solution.

PROGRAM SETUP

Program Name: POT Blank Low: 0.0000

Program Method: End Point Blank High: 2.5000
Main Filter: 578 Normal Low: 3.60003 mEq/L
Sub Filter: None Normal High: 5.50003 mEq/L
Program Unit: mEq/L Num of STD: 1
Aspiration Volume: 0500 µl CONC: 5.00001 mEq/L
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mEq/L
Dilution Factor: 1.0000 Control N Max: 0.00004 mEq/L
Linearity Min: 2.0000 mEq/L Control P Min: 0.00004 mEq/L
Linearity Max: 10.0000 mEq/L Control P Max: 0.00004 mEq/L
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum samples. For plasma samples; please check the insert for the values
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 10 mEq/l of Potassium.
If the obtained results are greater than 10 mEq/l, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB016
Sodium
VOL 1 x 60 ml
Colorimetric test. Mg-Uranylacetate
Standard 1 x 2 mL

REAGENT PREPARATION AND STABILITY

Reagents are ready to use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use. Handle standard very carefully to prevent contamination.
Precipitating solution becomes discolored when exposed to the light. Store protected from light. A slight turbidity does not affect
the determination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature. Then, pipette into a test tube:
Blank Sample Standard1
1
Standard 20 µl
Sample 20 µl
Reagent 2 1.0 ml 1.0 ml
Mix well. Incubate for 5 min. Then shake intensively for at least 30 sec. Then incubate for 30 min.
Centrifuge for 5-10 min at 5000 rpm. Collect the supernatant in a new test tube:
Blank Sample Standard1
Reagent 2 20 µl
Supernatant 20 µl 20 µl
Reagent 1 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 5 minutes at room temperature. After the incubation time,
aspirate and measure all the samples within 30 min after addition of the reagent 1.

PROGRAM SETUP
Program Name: SOD Blank Low: 0.0000
Program Method: End Point Blank High: 2.5000
Main Filter: 405 Normal Low: 135.0000 mEq/L
Sub Filter: None Normal High: 155.0000 mEq/L
Program Unit: mEq/L Num of STD: 1
Aspiration Volume: 0500 µl CONC: 150.00001 mEq/L
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mEq/L
Dilution Factor: 1.0000 Control N Max: 0.00004 mEq/L
Linearity Min: 49.0000 mEq/L Control P Min: 0.0000 4 mEq/L
Linearity Max: 300.0000 mEq/L Control P Max: 0.00004 mEq/L
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 300 mEq/l of Sodium.
If the obtained results are greater than 300 mEq/l, dilute the sample 1:2 with distilled water, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB018
Total lipids
VOL 2 x 125 ml
Colorimetric test
Standard 1 x 5 mL Phosphovainilla

REAGENT PREPARATION AND STABILITY

Reagent 1 is ready to use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.
Control sera are recommended to monitor the performance of assay procedures. If the control values are found outside the defined
range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard
Standard 100 µl
Sample 100 µl
Sulphuric acid 2.5 ml 2.5 ml
Shake thoroughly using a mechanical stirrer. Incubate for 10 min in a boiling water bath (100°C).
After that, cool in iced water and transfer into a new test tube:
Blank Sample Standard
Standard acid digest 50 µl
Sample acid digest 50 µl
Reagent 1 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Shake thoroughly using a mechanical stirrer. Incubate for 15 minutes at 37°C.
After the incubation time, aspirate and measure all the samples within 1 hour after addition of the reagent 1.

PROGRAM SETUP

Program Name: TLIP Blank Low: 0.0000²

Program Method: End Point Blank High: 0.3200²
Main Filter: 510 Normal Low: 450.0000 mg/dL
Sub Filter: None Normal High: 800.0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 750.0000 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 7.7000 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 1500.0000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 1500 mg/dl of lipids.
If the obtained results are greater than 1500 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and
multiply the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB0190 HB0190A HB0190M

Total protein
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Biuret. Colorimetric
Standard 1 x 5 ml 4 x 5 ml -

REAGENT PREPARATION AND STABILITY

The reagent is ready for use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
1
Standard 25 µl
Sample 25 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 minutes at 15-25°C or 5 min at 37°C. After the incubation
time, aspirate and measure all the samples within one hour after preparation.

PROGRAM SETUP
Program Name: TP Blank Low: 0.00002
Program Method: End Point Blank High: 0.22002
Main Filter: 546 Normal Low: 6.6000 g/dL
Sub Filter: None Normal High: 8.3000 g/dL
Program Unit: g/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: (value see vial)1 g/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 g/dL
4
Dilution Factor: 1.0000 Control N Max: 0.0000 g/dL
Linearity Min: 0.0080 g/dL Control P Min: 0.00004 g/dL
Linearity Max: 15.0000 g/dL Control P Max: 0.00004 g/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 15 g/dl of total protein.
If the obtained results are greater than 15 g/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB020
Total protein in Urine and CSF
VOL 2 x 125 ml
Colorimetric test. Pyrogallol Red
Standard 1 x 5 mL

REAGENT PREPARATION AND STABILITY

The reagent is ready for use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.
Control sera are recommended to monitor the performance of assay procedures. If the control values are found outside the defined
range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard
Standard 20 µl
Sample 20 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 minutes at 15-25°C or 5 min at 37°C. After the incubation
time, aspirate and measure all the samples within 30 min after preparation.

PROGRAM SETUP

Program Name: TPU Blank Low: 0.0000²

Program Method: End Point Blank High: 0.7000²
Main Filter: 578 Normal Low: 0.00003 mg/L
Sub Filter: None Normal High: 100.00003 mg/L
Program Unit: mg/L Num of STD: 1
Aspiration Volume: 0500 µl CONC: 1000.0000 mg/L
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/L
4
Dilution Factor: 1.0000 Control N Max: 0.0000 mg/L
Linearity Min: 9.4400 mg/L Control P Min: 0.00004 mg/L
Linearity Max: 4000.0000 mg/L Control P Max: 0.00004 mg/L
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 3
3
These values are for urine samples. For CSF samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 4000 mg/l of TPU.
If the obtained results are greater than 4000 mg/l, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB021
Triglycerides
VOL 12 x 20 ml
Enzymatic-colorimetric test.
Standard 1 x 5 mL GPO-POD

REAGENT PREPARATION AND STABILITY

Dissolve the contents of one bottle R2 Enzymes into the contents of one bottle R1 Buffer. Cap and mix gently to dissolve the
contents. This working reagent is stable 6 weeks at 2-8°C or 1 week at room temperature (15-25°C).
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 minutes at 15-25°C or 5 min at 37°C. After the incubation
time, aspirate and measure all the samples within 30 min after preparation.

PROGRAM SETUP
Program Name: TRIG Blank Low: 0.00002
Program Method: End Point Blank High: 0.14002
Main Filter: 510 Normal Low: 35.0000 mg/dL
Sub Filter: None Normal High: 160.0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 200.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 0.0000 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 1200.0000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 1200 mg/dl of Triglycerides.
If the obtained results are greater than 1200 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and
multiply the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HBL060 HBL060A HBL060M

Triglycerides Liquid
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Enzymatic. Colorimetric.
Standard 1 x 5 ml 4 x 5 ml - GPO-POD

REAGENT PREPARATION AND STABILITY

All the reagents are ready to use.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit.1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 10 µl
Sample 10 µl
Reagent 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 minutes at 15-25°C or 5 min at 37°C. After the incubation
time, aspirate and measure all the samples within 30 min after preparation.

PROGRAM SETUP

Program Name: TRIGLn Blank Low: 0.00002

Program Method: End Point Blank High: 0.23002
Main Filter: 510 Normal Low: 35.0000 mg/dL
Sub Filter: None Normal High: 150.0000 mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 200.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
Linearity Min: 1.0100 mg/dL Control P Min: 0.00004 mg/dL
4
Linearity Max: 1000.0000 mg/dL Control P Max: 0.0000 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 1000 mg/dl of Triglycerides.
If the obtained results are greater than 1000 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and
multiply the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HB022 HB029

Urea
VOL 4 x 250 ml 2 x 125 ml
Berthelot
Standard 1 x 5 ml 1 x 5 ml Enzymatic-colorimetric test

REAGENT PREPARATION AND STABILITY

R2 is ready for use.
Dissolve the content of one bottle R3 Enzymes into one bottle R1 Buffer. Cap and mix gently to dissolve the contents.
This working solution is stable 4 weeks at 2-8°C or 1 week at room temperature (15-25°C).

All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range2. Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
1
Standard 10 µl
Sample 10 µl
Working solution (R1+R3) 1.0 ml 1.0 ml 1.0 ml
Mix and incubate for 10 min at 15-25°C or 5 min at 37°C. And then add:
Reagent 2 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 min at 15-25°C or 5 min at 37°C. After the incubation
time, aspirate and measure all the samples within 30 min after addition of the reagent 2.

PROGRAM SETUP

Program Name: UREA Blank Low: 0.00002

Program Method: End Point Blank High: 0.32002
Main Filter: 578 Normal Low: 15.0000³ mg/dL
Sub Filter: None Normal High: 45.0000³ mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 50.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 0.0010 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 225.0000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 225 mg/dl of urea.
If the obtained results are greater than 225 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HBL03
Urea Liquid
VOL 160 + 40 ml
U.V. Kinetic test
Standard 1 x 5 ml Urease-GLDH

REAGENT PREPARATION AND STABILITY

Mix 4 volumes of R1 (Buffer) with 1 volume of R2 (Enzymes). After mixing, allow to stand for 30 minutes prior to use.
This working reagent is stable for 1 month at 2-8°C and 1 week at 15-25°C.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.
CALIBRATION & QUALITY CONTROL
Use the standard included in the kit1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Pre-rinse Standard1 Sample
1
Standard 20 µl 10 µl
Sample 10 µl
Working solution (R1+R2) 1.0 ml 2.0 ml 1.0 ml 1.0 ml
Before calibration, rinse the CYANSmart with the Pre-rinse mixture after 2 min incubation at 37°C using the “Wash” button.*
Prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart, immediately after addition of the
working solution to the sample/(calibrator). Wait with the preparation of the next sample until the CYANSmart is finished with
measuring the previous one. At this time, the CYANSmart will asks: “Press PUSH Aspirate Sample”. **

PROGRAM SETUP
Program Name: UREAL Blank Low: 1.0000²
Program Method: Two Point Blank High: 2.5000²
Main Filter: 340 Normal Low: 15.0000³ mg/dL
Sub Filter: None Normal High: 45.0000³ mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0800 µl CONC: 50.00001 mg/dL
Delay Time: 030 sec Factor: 0.0000
Test Time: 060 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 0.74300 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 400.0000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Reagent² Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned on the insert
provided with HBC03.
² Programming reagent as blank gives you the advantage that deterioration of the reagent can be easily detected. Alternatively, you can program
and aspirate distilled water as blank, with values for Blank Low: 0.0000 and Blank High: 0.0100.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control
LINEARITY
This method is linear up to 400 mg/dl of urea. If the obtained results are greater than 400 mg/dl, dilute the sample 1:2 with saline
solution, repeat the determination, and multiply the result by factor 2.

NOTES
*
Prerinsing the CYANSmart flowcell with the Pre-rinse mixture is essential to improve the calibration accuracy.
**
Carry-over by pathological samples is possible. To prevent this, we recommend to follow a sample with concentration >50 mg/dl
by aspiration of 0.8 ml Reagent Blank (working solution) before proceeding with the next sample.

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 CY009 CYANSmart
Application sheet

REF HB023 HB024

Uric Acid Lyophilized
VOL 12 x 20 ml 2 x 125 ml
Enzymatic-colorimetric test
Standard 1 x 5 ml 1 x 5 ml Uricase-POD

REAGENT PREPARATION AND STABILITY

Dissolve the contents of one bottle R2 to the contents of one bottle buffer solution R1. Cap and mix gently to dissolve the contents.
This working reagent is stable for 1 month at 2-8°C or 10 days at room temperature.
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Use the standard included in the kit1.
Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 25 µl
Sample 25 µl
Working solution (R1+R2) 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 min at 15-25°C or 5 min at 37°C. After the incubation
time, aspirate and measure all the samples within 30 min after addition of the working reagent.

PROGRAM SETUP

Program Name: UA Blank Low: 0.00002

Program Method: End Point Blank High: 0.16002
Main Filter: 510 Normal Low: 2.5000³ mg/dL
Sub Filter: None Normal High: 7.7000³ mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 6.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
4
Dilution Factor: 1.0000 Control N Max: 0.0000 mg/dL
Linearity Min: 0.0000 mg/dL Control P Min: 0.00004 mg/dL
4
Linearity Max: 40.0000 mg/dL Control P Max: 0.0000 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 40 mg/dl of uric acid.
If the obtained results are greater than 40 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

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 CY009 CYANSmart
Application sheet

REF HBL020 HBL020A HBL020M

Uric Acid Liquid
VOL 2 x 125 ml 8 x 125 ml 8 x 30 ml
Enzymatic-colorimetric test
Standard 1 x 5 ml 4 x 5 ml - Uricase-POD

REAGENT PREPARATION AND STABILITY

Mix equal volumes of R1 buffer and R2 Enzymes.
This working reagent is stable for 2 months at 2-8°C or 2 weeks at room temperature (15-25°C).
All the components of the kit are stable at 2-8°C up to the date of expiration as specified, when stored tightly closed, protected
from light and contaminations prevented during their use.
The reagent should be discarded if turbidity or precipitation has occurred or if the blank absorbance is out of range². Handle
standard very carefully to prevent contamination.

CALIBRATION & QUALITY CONTROL

Control sera are recommended to monitor the performance of assay procedures. Use Biochemistry Normal and Pathological
Controls (HBC01, HBC02). Prepare and measure these controls the same as samples. If the control values are found outside the
defined range, mentioned on the insert of the control, check the instrument, reagents and calibration for problems.

SAMPLE PREPARATION
Use the standard included in the kit1.
Make sure the reagents and samples are at room temperature.
Then, pipette into a test tube:
Blank Sample Standard1
Standard1 25 µl
Sample 25 µl
Working solution (R1+R2) 1.0 ml 1.0 ml 1.0 ml
You can prepare several samples simultaneously. Mix and incubate for 10 min at 15-25°C or 5 minutes at 37°C. After the incubation
time, aspirate and measure all the samples within 45 min after addition of the working reagent.

PROGRAM SETUP
Program Name: UAL Blank Low: 0.00002
Program Method: End Point Blank High: 0.12002
Main Filter: 510 Normal Low: 2.5000³ mg/dL
Sub Filter: None Normal High: 7.7000³ mg/dL
Program Unit: mg/dL Num of STD: 1
Aspiration Volume: 0500 µl CONC: 6.00001 mg/dL
Delay Time: 001 sec Factor: 0.0000
Test Time: 003 sec Control N Min: 0.00004 mg/dL
Dilution Factor: 1.0000 Control N Max: 0.00004 mg/dL
4
Linearity Min: 0.0600 mg/dL Control P Min: 0.0000 mg/dL
Linearity Max: 25.0000 mg/dL Control P Max: 0.00004 mg/dL
Blank: Reagent Cuvette Temp: 37 °C

Num of Blank: 1
1
Alternatively, you can use the Biochemistry Calibrator (HBC03) for calibration. Program NUM of STD: 1 and CONC: as mentioned
on the insert provided with HBC03.
3
These values are for serum or plasma samples. For urine samples; please check the insert for the values and sample preparation.
4
The control values can be found on the control sheets delivered together with the control

LINEARITY
This method is linear up to 25 mg/dl of uric acid.
If the obtained results are greater than 25 mg/dl, dilute the sample 1:2 with saline solution, repeat the determination, and multiply
the result by factor 2.

Version 20191127 - replaces all previous versions 53

 CY009 CYANSmart
Application sheet

LIN340
Once every year, CYANSmart should be validated to assure correct results.

Validation of CYANSmart is based on two principles:

 Verification of linearity
 Verification of precision
This is done by measuring two reference solutions at different wavelengths:
 Potassium dichromate at 340 nm using the LIN340 method
 Ammonium cobalt(II) sulfate hexahydrate at 510 nm using the LIN510 method

PREPARATION AND STABILITY

Prepare a dilution series of Potassium Dichromate K2Cr2O7 as measuring solution.
See service manual for detailed procedure.

PROGRAM SETUP

Program Name: LIN340 Blank Low: 0.0000

Program Method: End Point Blank High: 0.0100
Program Unit: g/l Normal Low: 0.2000
Main Filter: 340 Normal High: 1.7000
Sub Filter: None NUM of STD: 0
Delay Time: 001 sec CONC: 0.0000
Test Time: 003 sec Factor: 1.0000
Aspirate Volume: 0500 µl Control N Min: 0.0000
Dilution factor: 1.0000 Control N Max: 0.0000
Linearity Min: 0.0050 Control P Min: 0.0000
Linearity Max: 2.5000 Control P Max: 0.0000
Blank: Water Cuvette Temp: 37 °C
Num of blank: 1

Version 20191127 - replaces all previous versions 54

 CY009 CYANSmart
Application sheet

LIN510
Once every year, CYANSmart should be validated to assure correct results.

Validation of CYANSmart is based on two principles:

 Verification of linearity
 Verification of precision
This is done by measuring two reference solutions at different wavelengths:
 Potassium dichromate at 340 nm using the LIN340 method
 Ammonium cobalt(II) sulfate hexahydrate at 510 nm using the LIN510 method

PREPARATION AND STABILITY

Prepare a dilution series of Ammonium Cobalt(II) Sulfate Hexahydrate as measuring solution.
See service manual for detailed procedure.

PROGRAM SETUP

Program Name: LIN510 Blank Low: 0.0000

Program Method: End Point Blank High: 0.0100
Program Unit: g/dl Normal Low: 0.3000
Main Filter: 510 Normal High: 1.9000
Sub Filter: None NUM of STD: 0
Delay Time: 001 sec CONC: 0.0000
Test Time: 003 sec Factor: 1.0000
Aspirate Volume: 0500 µl Control N Min: 0.0000
Dilution factor: 1.0000 Control N Max: 0.0000
Linearity Min: 0.0050 Control P Min: 0.0000
Linearity Max: 2.5000 Control P Max: 1.0000
Blank: Water Cuvette Temp: 37 °C
Num of blank: 1

Version 20191127 - replaces all previous versions 55

 CY009 CYANSmart
Application sheet

Adjustments compared to the previous version 20171123

1. Urea Liquid
- Pre-rinsing for improved accuracy
- Aspiration volume: 800 µL
- After-rinsing for pathological samples to prevent carry-over
2. Cholesterol HDL Direct  NEW
3. Cholesterol LDL Direct  NEW
4. Cholesterol Liquid old formulation (CHOLL)  Discontinued, now use method CHOLLn
5. Triglycerides Liquid old formulation (TRIGL)  Discontinued, now use method TRIGLn
6. Iron + TIBC
- Blank High: 0.1000
7. GOTL
- Stability working solution: 14 days at 2-8°C

Adjustments compared to the previous version 20180305

1. Albumin  HB0010 (1L format) discontinued, use HB001
2. Cholesterol HDL direct (HBL011)
a. Reference ranges
b. Improved explanation and addition of illustrations on for a time-efficient way to test several samples
simultaneously

3. Cholesterol LDL direct (HBL012)

a. Reference ranges
b. Improved explanation and addition of illustrations for a time-efficient way to test several samples
simultaneously
4. Cholesterol LDL direct (HBL012)
5. Cholesterol LDL (HB028)  Discontinued, now use method LDLd
6. SODenz  Discontinued, use method SOD
7. Triglycerides (HB021)
a. Linearity limit: 1200 mg/dL

Adjustments compared to the previous version 20181119

1. Glucose Liquid (HBL04/HBL04A)  New improved formulation
Starting from lot GL-00731, use GLUCLn method

Adjustments compared to the previous version 20190401

1. HDL precipitation (HB007)  Discontinued
2. Lactate dehydrogenase (HBE08)  Discontinued
3. HbA1c (HT001) Sample preparation:
a. Sample volume 15µL
b. Reagent 1 volume 540 µL
c. Reagent 2 volume 180µL

Version 20191127 - replaces all previous versions 56

 CY009

English
To all Healthcare Professionals,

Thank you for your interest. We appreciate it!

CYANSmart
Cypress Diagnostics provides the tools and solutions that
let clinical laboratories worldwide deliver clear and precise
diagnostic results.
We manufacture the equipment needed for handling the

Semi-Automatic Biochemistry
samples, analyzers to perform the tests, reagents to carry out
the tests and - increasingly often - the software platforms to
report the results.

Analyzer Cypress Diagnostics supplies clinical laboratories.

We are a quality-oriented company with ISO certification

and CE-approved products. Our production facility is located
in Hulshout, Belgium. Our products are preferred and
appreciated by users in more than 100 countries worldwide.
Cypress Diagnostics is a family-owned business, founded in
1995 in Leuven, Belgium.

We work exclusively with selected and trained distributors.

We sell high-quality instruments and customer service is an
integral part of our quality policy.
All our products come with an aftersales and performance
guarantee.

Please feel free to contact our local distributor for more

information.
Our latest inserts and catalogs are downloadable on
www.diagnostics.be .

Test the difference!

Kind regards,

The Cypress Diagnostics Team

ISO 13485-2016
www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: cypress@diagnostics.be
CYANSmart Technical Specifications

Optical system Power supply

The CYANSmart is an an easy-to-operate semi-automatic biochemistry analyzer. This new in vitro diagnostic • Flow cell: 32 µl, 10 mm light path • AC 110/220 V - 50/60 Hz
instrument is designed to have powerful features in a compact, independent unit. • Min. reaction volume: 500 µl per test • Automatic voltage and frequency switch
• Halogen lamp 6V/10W • Grounding required
• Photo detector: Silicon based (range 300 – 900 nm) • Power: 200 VA, reduced to 140 VA in standby mode
Cost savings: Convenience:
• Measurement range: 0,000 – 3,500 Abs • Power save / Standby mode
• Minimum reaction volume of 500 µl means decreased • Easy tracking of all the results from each patient by a
• Wavelength: 340 – 620 nm
costs per test unique identifier
• Wavelength selection: Automatic via 7 interference Software
• Limited consumables required • All Cypress Diagnostics biochemistry reagents are
filters: 340, 405, 492, 510, 546, 578 & 620 nm • Unique 16 digit patient ID
• Extra items included on delivery (two fuses, five paper preprogrammed on delivery
• Memory: 180 test methods and 6000 sample test results
rolls, a spare lamp, and pump tube) • 180 open channels are programmable to allow for
Flow cell • Cypress Diagnostics methods preprogrammed on
• Integrated incubator, printer, pump, and flow cell other user-defined tests
• 25 °C, 30 °C, 37 °C, ambient temperature delivery
• Long life lamp, further extended by power saving / • Memory for up to 6000 sample results
• Peltier element • Languages: English, French, Spanish
standby mode • Large font size, easily legible and read with little effort
• Thermostatic control: PID controlled, ±0,5 °C • QC: 2 controls programmable plus a separate QC results
• Robustness of the analyzer with minimal daily • LCD touch screen with virtual alphanumeric keyboard
• 10 mm menu with (printable) graphs
maintenance • Multilingual capability
• Calculation methods: absorbance, endpoint, two-point,
• Analyzer guides the user and so reduces mistakes • Minimal daily maintenance
Incubator kinetic, bichromatic
• 20 places with 14 mm diameter • Calibration methods: factor, calibrator and multi-point
Flexibility:
• 37 °C calibration
• On-board printer for printing automatically or on Desktop Software:
• Thermostatic control: PID controlled, ± 1 °C • Blank options: sample blank, reagent blank and water
request printing • Clear reporting with patient overview with flags and
• Incubation time: 0 – 999 seconds
• Seven wavelengths (340, 405, 492, 510, 546, 578, and reference ranges
Display • Reading time: 3 – 999 seconds
620 nm filters) cover all clinical diagnostic applications • Easier reporting and result sharing (email & network
• Back-illuminated LCD
• Five calculation methods (absorbance, endpoint, printing)
• Touchscreen calibration possible Weight and dimensions
two-point, kinetic, bichromatic) and three calibration • Elimination of transcription errors (no more manual
• 800 x 480 pixels (screen size: 7 inch) • Instrument: 9 kg, 20 x 43,5 x 41,5 cm (H x W x L)
methods (factor, calibrator, and multi-point data entry)
calibration) provide plenty processing options with • Data back-up
Printer Environmental requirements
one system • Convenient upgrade of the methods by quick upload
• Automatic or on-demand printing • Ambient temperature: 15 °C – 30 °C
• Three blank options (sample blank, reagent blank, and • Built-in thermal printer • Relative humidity: 30 % - 70 %
water) provide background correction of the reagent • 24 characters per line
and sample coloration • Prints graphs Order code:
• CY009: CYANSmart

Contact: e-mail: cypress@diagnostics.be Your Distributor

tel: + 32 (0)15 67 67 68
Nijverheidsstraat 8
2235 Hulshout
Belgium
URL: www.diagnostics.be CYANSmart EN 2019-04

ISO 13485-2016 ISO 13485-2016

www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: cypress@diagnostics.be www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: cypress@diagnostics.be