Activity 1 Oh My Gas!: Hydrilla Sprig

ACTIVITY 1
OH MY GAS!
Introduction
Certain organisms possess the ability to capture solar energy and utilize it
for the synthesis of organic compounds. This process which involves a series of
enzyme-mediated complex reaction is photosynthesis. The organic compounds
from photosynthesis would later serve as source of energy for the cells or as
materials for building cellular components.
In this activity, you will be observing the taking-off and release of gases that
is involved in the process of photosynthesis.
Objective
At the end of this activity you are expected to provide evidence that plants
take-in and release-off gas molecules during the process of photosynthesis.
PART A: GAS UPTAKE
Materials
Water
Hydrilla sprig
Bromthymol blue
Straw
Beaker
Test tube
Light source
Procedure
1. Obtain a 250 mL beaker and fill it with 150 mL tap water. Place two to three
drops of Bromthymol blue. Stir well with a strring rod. Bromthymol blue
is an indicator that changes color depending on the amount of carbon
dioxide dissolved in solution.
2. Introduce carbon dioixide in the solution by blowing a straw until a color
change from blue to lig
ht yellow is observed.
3. Pour xxxx mL of the prepared water-Bromthymol blue solution in two test

tubes. Label it test tubes A and B. For test tube A, add sprigs of Hydrilla.
4. Let the test tubes remain under bright sunlight or artificial light for 24 hours.
Describe what happened to the setups.
Guide Questions
Q1. Describe what you observed in test tubes A and B after 24 hours.
Q2. Explain the reason behind the change in color that happened to the solution
after 24 hours.
PART B: GAS RELEASE
Materials
 1000 mL beaker
 3 g sodium bicarbonate
 Hydrilla sprig
 Funnel
 Test tube
Procedure
1. Half-fill a 1000 mL beaker with tap water.

2. Add 3 g of sodium bicarbonate.
3. Place Hydrilla sprig in the bottom of the beaker.
4. Put a funnel over the plant, as shown:
5. Fill the test tube with water up to its brim. Secure the mouth of the test tube
with your thumb. Invert the tube and place it on top of the funnel.
6. Place the beaker under a light source. Observe what will happen from the
funnel with Hydrilla up to the inverted test tube.
Guide Questions
Q3. Describe what you observed in the test tube after placing it inverted on top
of the funnel with Hydrilla inside?
Q4. What does the presence of bubbles represent? What is inside the bubbles?
Q5. The purpose of adding sodium bicarbonate powder is to increase the
amount of carbon dioxide in the water. What is the role of carbon dioxide in the
process of photosynthesis?
Q6. Discuss the exchange of gases happening during photosynthesis (discuss
the uptake and release).
ACTIVITY 2
BLOW IT UP!
Introduction
Cellular respiration is a process of converting food molecule to an energy form

that can be used. It has two types:
1. Aerobic Respiration- happens when oxygen is present

2. Anaerobic Respiration- happens even without oxygen, requires enzymes or
microorganisms that can hasten the reactions.
The following activity will let you explore the process of cellular respiration by
identifying the materials and products involved in this life energy process.
Objective
At the end of this activity you are expected to observe cellular respiration of
yeast in an enclosed, fluid environment.
Materials
A packet of yeast
Erlenmeyer flask
Table sugar
Water
Latex balloon
Procedure
1. Fill two Erlenmeyer flasks with XXXX mL of water. Label the flasks with A
and B.
2. Add one teaspoon of yeast to the two flasks and gently swirl the bottle a few
seconds.
3. Add one teaspoon of table sugar to flask A and swirl it around some more.
Below is the summary of the contents in each of the flasks:
Erlenmeyer flask A: water, yeast, and table sugar
Erlenmeyer flask B: water, and yeast
4. Stretch out the balloon and place the neck of the balloon over the neck of the
Erlenmeyer flask. Do this to both of the setups.
5. Let the flasks sit in a warm place for about 20 minutes. Observe what will
happen to the balloons.
6. Remove the balloon and take note the resulting smell of the solution.
Guide Questions
Q1. What happened to the balloon in the setup containing water, yeast, and
sugar? How about in the setup with water and yeast only?
Q2. What do you think is inside the inflated balloon? Predict its possible
identity.
Q3. Describe the smell of the resulting solution and compare it to a commercial
beverage.
Q4. The experiment is an example of a cellular respiration taking place, is the
setup an example of aerobic respiration or anaerobic respiration? Why do you
say so?
Q5. What is/are the starting material/s of anaerobic respiration?
Q6. What is/are the end product/s of anaerobic respiration?
References
All Science Fair Projects. <http://www.all-science-fair-

projects.com/phase2/app/webroot/files/image/ 00410/b1.jpg> accessed 23 May 2015
Biology Pre-IGCSE. <https://michelleswpr.files.wordpress.com/2013/11/starch-test-boil-leaf-in-

ethanol.jpg> accessed 23 May 2015
Blow up a balloon with yeast. http://sciencebob.com/blow-up-a-balloon-with-yeast/ accessed 23

May 2015
Duka, Ivan-Marcelo A., Maribel T. Joson-Villegas & Ma. Genaleen Q. Diaz. 2003. Biology 1
Laboratory Manual: An Investigative Approach. 7th ed. University of the Philippines Los
Banos: Institute of Biological Sciences.
Olivares, Maria, Ma. Eloisa Bermio & Juanita Cruz. 2003. Science and Technology for the
Modern World. Makati City: Diwa Scholastic Press Inc.
Photosynthesis. <http://mypages.iit.edu/~smile/bi9201.html> accessed 23 May 2015
University of the Philippines National Institute for Science and Mathematics education
Development. 2009. Biology: Science and technology Textbook for Second Year.
Mandaluyong City: Book Media Press, Inc.

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ACTIVITY 1

PART A: GAS UPTAKE

3. Pour xxxx mL of the prepared water-Bromthymol blue solution in two test

PART B: GAS RELEASE

1. Half-fill a 1000 mL beaker with tap water.

Cellular respiration is a process of converting food molecule to an energy form

1. Aerobic Respiration- happens when oxygen is present

All Science Fair Projects. <http://www.all-science-fair-

Biology Pre-IGCSE. <https://michelleswpr.files.wordpress.com/2013/11/starch-test-boil-leaf-in-

Blow up a balloon with yeast. http://sciencebob.com/blow-up-a-balloon-with-yeast/ accessed 23

Photosynthesis. <http://mypages.iit.edu/~smile/bi9201.html> accessed 23 May 2015

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