MS9-5 Reference Book PDF

MELET SCHLOESING Laboratoires MS9-5® Reference Manual / 09-02 Page : 1
MS9-5
Automatic
Full Digital
Cell Counter
REFERENCE MANUAL
Reference Manual
Version 1.0
BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

Page : 2 MS9-5® Reference Manual / 09-02 MELET SCHLOESING Laboratoires
VERY IMPORTANT
It is VERY DANGEROUS for the MS9-5 electronic parts, MS9-5 delivered
analysis results and for the MS9-5 operator safety to connect the MS9-5
analyzer on a main power supply which does not have any Ground (Earth).
The Phase, Neutral and Ground (3rd connector on the plug) need to be really
supplied from a normalized plug certified by a professional installer. Not only
for the security of the operator, the need and quality of the Ground value
gives also to the MS9-5 analyzer the reference zero voltage calibration used
to realize hematology analysis. No Guarantee and/or Warranty could be applied
if the MS9-5 is not properly connected to a normalized Earthed and safe
electric installation.

CONTENTS

Page
UNPACKING 7
Transport Box 8
Unpacking the MS9-5 10
The Instrument 12
EXTERNAL OVERVIEW 15
Front View 16
Right View 18
Left View 20
Back View 22
Connection Panel 24
INSTALLATION 27
Cables 28
Internal Preparation 30
Reagent Tubes 32
MS-Card 34
Reagents Pack 36
INSIDE OVERVIEW 39
Fluidic Front View 40
Fluidic Back Parts 42
Electronic Parts 44
FLUIDIC DETAILS 47
Fluidic parts 48
Fluidic diagram 50
Diluent circuit 52
Detergent circuit 54
Lyse circuit 56
Lyse Eo circuit 58
Hb Meas. / Ref. circuit 60
Press. / vacuum circuit 62
Emptying chambers circuit 64
Emptying waste chamber / Vacuum cleaner circuit 66
Predilution circuit 68
The peristaltic pump 70
Valve actions 72
ELECTRONIC DETAILS 75
Boards Implantation 76
Amplifier board, External view 78
Amplifier board, Internal view 80
Connection boards 82
FK1-E Fluidic board 84
CK1-E Acquisition board 86
../..

Page
ELECTRONIC DETAILS ../..
Mother board 88
LCD TTL buffer 90
Color LCD screen cable 92
LCD screen cable adapter 93
Power Supply 94
Electric diagram 96
Ground diagram 98
General electronic diagram 100
CELL MEASUREMENT 103

Principle 104
Probe voltage calibration 106
Pulse amplification 108
Pulse acquisition 110
Pulse dimensions 112
Electronic filters 114
Histograms 116
Histogram smoothing 118
Hb photometer principle 120
DILUTION 123
Principle 124
Dilution cycle data 126
Dilution cycle steps 129
COUNTING 137
Principle 138
Counting cycle data 140
RESULTS 145
Result measurement 146
Eosino calibration 148
Result correction 150
Result calculation 152
Analytical parameters 154
Plt histogram deviation 156
3 Part-Diff parameters 158
3/5 Part-Diff parameters 160
5 Part-Diff parameters 162
ERROR CODES 167

Fluidic Error Codes 168
FK1-E Error Codes 169
CK1-E Error Codes 170
../..

REF. SPARE PARTS 173

Complete parts 174
Electronic parts 175
Solenoid parts 176
Plastic parts 177
Metallic parts 178
Ring parts 179
Tube parts 180
Computer parts 181
Accessories 182
BIOS SETUP 185

AWARD BIOS Setup 186
ANNEXES 191
Index 192

UNPACKING

TRANSPORT BOX
The 'transport box' is made to prevent any kind
of shock. Two fast opening manual locks on front
side give easy access to the interior.
Unlock the two latches.
Open the lid to access to MS9-5 and

accessories.
Notes :


Keyboard
Manuals
Accessories

UNPACKING THE MS9-5

Take out the plastic pocket with the
manuals which can be found on the right.
To extract the MS9-5 from the transport box,

tilt it backwards, holding the bottom of the
front part and the top side of the back part
as shown on the picture.
Take the keyboard and the accessories

box out.
Store the transport box in a safe place for

further use, once all the elements have
been extracted.
Notes :


THE INSTRUMENT
The instrument is packed in the box with 2 plastic Notes :
pockets and one accessories box which include :
- One MS-Card
- keyboard
- Reference manual / Quick tour manual
- reagent tubes with power cord, cover
keys and whole blood tubes adapters
Pull out by hands the protection plastic film which

covers the instrument. Never use a knife at this
stage.
Check accessories as follows :
1 alphanumeric keyboard inside a plastic

pocket,
1 mouse with a Y adapter,
2 plastic pockets with :

- 5 reagent tubes
- 1 orange reagent tube
- 1 cleaning solution,
- 1 power cord,
A plastic pocket with :

- 1 Reference manual
- 1 Quick tour manual
A plastic pocket with :

- 4 blood clot filters
- 5 whole blood tubes adapters
- 2 keys for MS9-5 front cover
A blank CD-RW.



EXTERNAL OVERVIEW

FRONT VIEW
A front description of the MS9-5 shows :
A large polychrome back lighted

VGA LCD screen,
A plastic front cover,
A whole blood tube holder which is

also the analysis starting place,
A compact alphanumeric keyboard.
Notes :



RIGHT VIEW
A right profile description of the MS9-5 shows:
An upper cover,
Insert the key inside the lock to open

and lock the upper cover,
A CD-R/RW drive,
A whole blood tube holder which is

also the analysis starting switch,
A hole for the whole blood sampling

probe.
Notes :



LEFT VIEW
A left profile description of the MS9-5 shows :
An upper cover,
6 plastic Luer lock fittings with cap

on for the reagents tube connection,
1 exhaust tube for air transfer,
A multi connection panel.
Notes :



BACK VIEW
A back side description of the MS9-5 shows :
Hinges for the upper front cover,
Internal fixation holes,
Electric main power supply plug ()

and ON/OFF switch () with fuse
(),
Air flow filter.
Fuse type which must be used :

T 2,5A / 250 VAc
T 5A / 125 VAc
Notes :



CONNECTION PANEL
The multi-connection panel description of the Notes :
MS9-5 shows from top to bottom :
External DIN keyboard connector

Used only for technical purpose,
2 USB connectors,
DB9 serial connector COM2,
Parallel printer connector LPT1,
External VGA video monitor

connector,
LAN RJ45 connector (10/100 Mb/s

Ethernet),
The 'MS-Card' reader on FK1-E

board,
The CK1-E board,
PS/2 connector for mouse AND

keyboard :
NEVER PLUG THE MOUSE ON PS/2

CONNECTOR WITHOUT Y ADAPTER
DB9 serial connector : COM1.
ONLY CONNECT PERIPHERAL

INSTRUMENTS WHICH ARE COMPLIANT
WITH REF.: CEI 950, CEI 1010-1.




INSTALLATION

CABLES
The first stage of the MS9-5 installation is to
connect cables as follows :
Connection of the alphanumeric keyboard

under the front panel of the instrument.
Respect the right keyboard DIN plug
position when connecting.
Never plug the keyboard with the Y adapter
,, Connection of the mouse (with Y

adapter).
Connection of the power supply cord.

First check the quality of the current on
the wall plug.
Phasis...Neutral : 220 V or 110 V +/- 10%
Neutral...Ground : 1 V +/- 1 V
Phasis...Ground : = Phasis...Neutral
If these values are not reached it is

fundamental and strictly necessary to
request a better main power installation by
a professional. Earthing the MS9-5 is a basic
need for safety, good results and
Manufacturer guarantee and warranty
application.
If an external printer is used with the MS9-5,

both devices (MS9-5 + printer) must be
connected on the same multi-plug.
Notes :



INTERNAL PREPARATION
Some internal preparation must be done before
taking any further steps :
Put the keys on the lock on the right side

of the instrument. If there are any difficulties
in installing the keys simply twist it gently
when inserting them.
Open the upper front cover by turning the

key counterclockwise.
Unscrew the plastic cap of the reagent

luers (on the bottom left part of the MS9-5)
in a counterclockwise direction and take
them off.
Keep these caps in a safe place.
Take the 4 transportation stickers off (yellow

arrows on the picture).
Notes :

,

REAGENT TUBES
The reagent tubes must be connected as follows :
Check that the plastic protection cap of

the reagents tube connection luers on the
instrument have been taken out.
Install by screwing in a clockwise direction

each Luer lock on the tubes according to
its corresponding color.
Check the reagent tubes and color

positions from the front side to the back
side as follows :
Black : Waste
Orange : EOSINO Lyse
Yellow : Lyse
White : Diluent
Red : Hemoref
Blue : Detergent
Green : Air transfer
Notes :


MS-CARD
The reagents pack or batch are controlled by a
smart card called the MS-CARD.
Open the MS-Card carton pocket placed

on the side of the reagents pack or in the
lysing reagent carton box.
The plastic MS-Card holder appears.
Take the plastic MS-Card holder out and

close the MS-Card carton pocket.
Insert the MS-Card in the card reader

located at the middle place of the multi-
connection panel.
The MS-Card must be inserted following
the direction of the arrow drawn on the card
itself and with the printed side on top as
shown on the picture.
Notes :


REAGENTS PACK
For installation of the reagents pack or batch
proceed as follows:
As for the smart card MS-Card, open the

carton precut area on top of the pack or
on top of each reagent carton box. Each
reagent precut area is identified with a
colored sticker of the same color as the
Luer lock fitting on the reagent tube.
4 apertures will be seen +1 for Eo-Diff.
Unscrew the reagent bottles caps which

are now accessible.
Insert the reagent tubes with their cap in

each bottle. Check the identification color
(See reagent tubes section p.32).
Install the waste container and place it

underneath the table.
It is very recommended to install the

MS-Pack reagents for MS9-5 on the same
level as the MS9-5.
To use the reagents in another place, such
as underneath the table, the setup fluidic
program must be adapted.
Notes :



INSIDE OVERVIEW

FLUIDIC FRONT VIEW

A front description of the fluidic panel shows:
The dilution syringe pump,
The X/Y system with the sampling probe,
The whole blood tube holder,
The predilution cuvette,
The reagent level sensor block,
The WBC measuring chamber

block,
The RBC & Eo measuring
chambers block,
The hemoglobin photometer,
The optical coding wheel.
Notes :


FLUIDIC BACK PARTS

A description of the fluidic back panel shows:
The diluent solenoid valve,
The solenoid valves block,
The upper connection board,
The up/down stepper motor,
The waste chamber,
The syringe pump stepper motor,
The rotation stepper motor,
The peristaltic pump motor,
The amplifier board,
The waste solenoid valve,
The lower connection board.
Notes :


ELECTRONIC PARTS
A description of the electronic parts shows:
The power supply cooling fan,
The power supply,
The main power switch,
The CD-R/RW drive,
The main pump,
The boards CK1-E (acquisition) and

FK1-E (fluidic),
The main cooling fan,
Power for the connection board,
The standard keyboard plug,
The passive bus,
The mother board,
The multi connection panel,
The LCD screen cable.
Notes :




FLUIDIC DETAILS

FLUIDIC PARTS
The description of the fluidic parts shows :
The solenoid valves,
The sampling probe,
The syringe block,
The lysing reagent buffer tube support,
The hemoglobin photometer,
The waste chamber,
The peristaltic pump,
The measuring chambers block,
The predilution cuvette,
The level sensor block,
The pump with pressure sensor :

air input
air output
pressure sensor CPR1 on FK1-E
board.
Notes :



FLUIDIC DIAGRAM
The functional parts are described in the fluidic
diagram as follows :
1) Sensor or
ground connection :
2) Ground connection :
3) Blood clot filter :
4) Tube-to-tube connector :
5) NC means valve Normally Closed

NO means valve Normally Opened.
Notes :

Sensor

DILUENT CIRCUIT
The diluent circuit is described as follows :
1) From the external white reagent tube

female luer, to the reagent level sensor
block.
2) From the reagent level sensor block to

the valve 10 which when activated leads
the diluent to valve 11 to carry out the
dilution.
3) Valve 7 provides diluent to the peristaltic

pump during the cleaning of the probe.
4) Valve 11 dispatches the diluent to the

internal part of the sampling probe for
dilution or to the outside of the sampling
probe for a sleeve cleaning or dilution.
Notes :
Diluent level detection is done between the

level sensor block ground and the diluent
input of the block


DETERGENT CIRCUIT
The detergent circuit is described as follows: In 3-part mode, the detergent is not
led into the Eo chamber but directly
1) From the external blue reagent tube goes from the valve 22 to the waste
female luer, to the reagent level sensor chamber.
block.
2) From the reagent level sensor block

to a metallic straight connector which is
the electric ground for reagent level sensor;
then the tube goes to the valve 8 which
when activated allows the detergent to flow
in the lateral chambers of the measuring
chambers blocks. The first chamber rinsed
by the detergent must be the RBC lateral
chamber (on the right and rear side), then
the WBC lateral chamber and through the
valve 22 to the Eo lateral chamber.
3) Then from the Eo lateral chamber to

valve 1 which when activated allows the
detergent to be sucked to the waste
chamber.
4) During the counting cycle, only valve 1

(5-Part mode) / valve 22 (3-Part mode) is
opened and the waste chamber is under
vacuum.
5) During the back flushing process when

dilution is performed only valve 1 (5-Part
mode) / valve 22 (3-Part mode) is opened
with the waste chamber under pressure.
Notes :
Detergent level detection is done between

the detergent input of the level sensor block
and with an external ground located near
valve 8.


LYSE CIRCUIT
The lysing reagent circuit is described as follows:
1) From the external yellow reagent tube

block.

valve 6 which when activated allows the
lysing reagent to go to the WBC chamber.
On the NC way of valve 6 is a connection
with the predilution which is transferred
by valve 15 just above.
3) The lysing reagent is stocked in a buffer

tube.
4) The lyse piston is used to pull or push the

lysing reagent.
Notes :
Lyse level detection is done between the

level sensor block ground and the lyse input
for the block.
A reagent level sensor controls the lysing

reagent when it is stored inside the buffer
tube.


LYSE EO CIRCUIT
The lysing Eo reagent circuit is described as
follows:
1) From the external orange reagent tube

block.

valve 20 which when activated allows
the lysing reagent to go to the Eo
chamber.
3) The lyse Eo piston is used to push or pull

the lyse Eo reagent.
Notes :
Lyse Eo level detection is done between

the level sensor block ground and the lyse
Eo input of the block.


HB MEAS. / REF. CIRCUIT

The hemoglobin reference and measuring circuit
is described as follows :
1) From the external red reagent tube

block.
2) From reagent level sensor block to valve 5

and 7.
3) To prime the Hb photometer with some

Hb reference solution valve 5 and 17 are
activated under vacuum, then the blank
reference measurement can be carried
out.
4) To read the Hb value from a blood,

the valve 16 is activated to empty
the WBC chamber and valve 17 is
activated to switch the dilution to the
Hb photometer.
Notes :
Hb reference level detection is done

between the level sensor block ground and
the Hb reference input for the block.
The connection of Hb reference with valve 7

is described inside the peristaltic pump
section.


PRESS. / VACUUM CIRCUIT

The pressure/vacuum circuit is described as
follows:
1) From the pump head on which are

drawn arrows to indicate the air flow
direction (pressure and vacuum) to the
valves 13 and 14.
2) These valves (13-14) are both connected

to the waste chamber which is the main
vacuum and pressure fluidic supplier.
3) The pressure and vacuum are under

feedback control by means of valve 21
and a pressure sensor built in the fluidic
board FK1-E described further.
Notes :
Valve 13 is used to create pressure inside

waste chamber, valve 14 to create vacuum.


EMPTYING CHAMBERS
CIRCUIT
The WBC/RBC/Eo chambers emptying circuit
is described as follows:
1) RBC chamber is emptied by means of

valve 2, WBC chamber is emptied by
means of valve 16 and Eo chamber is
emptied by means of valve 19 when the
waste chamber is under vacuum.
2) To prime the Hb photometer with WBC

dilution the valves 16 and 17 are
activated with the waste chamber under
vacuum.
Notes :


EMPTYING WASTE CHAMBER/

VACUUM CLEANER CIRCUIT
The waste chamber emptying and sampling
probe vacuum cleaner circuits are described as
follows :
1) When the waste chamber is under

vacuum the external part of the sampling
needle can be sucked by means of valve 3.
Aspiration is done inside the needle
cleaning head.
2) When the pump is under pressure the main

waste chamber can be emptied by
means of the valve 4.
Notes :


PREDILUTION CIRCUIT
The predilution transfer circuit is described as
follows :
1) The WBC measuring chamber is under

vacuum by means of the valve 12 which
is activated.
2) When vacuum of transfer is correct

(under control of vacuum sensor), the
valve 15 is activated.
3) During the predilution transfer some lysing

reagent can be added from the syringe
and via valve 6 to the predilution circuit.
4) During dilution inside PDL cuvette the

peristaltic pump is used to create air
bubbles to improve dilution.
Notes :
A sensor located above WBC measuring

chamber is used to control the end of the
transfer.


THE PERISTALTIC PUMP

The peristaltic pump has two ways :
1) The first way is shared :
a) After each blood sampling valve 9 is

activated and diluent is carried out
by the peristaltic pump to the needle
to rinse its external part.
b) When the MS9-5 goes to standby

mode, valves 7 and 9 are activated.
It allows the peristaltic pump to drain

Hb ref. solution to the exterior of the
sampling probe, to wash it and take
salt crystals away.
This action is necessary and obligatory to

avoid any damage to the needle cleaning
head.
2) The peristaltic pump has an air intake on

the other way. Its purpose is the generation
of bubbles in the predilution cuvette to
improve the dilution of the blood.
Notes :


VALVE ACTIONS
The action of each valve is described as follows : Notes :
valve N° Action
1 Aspiration of detergent
Aspiration of dilutions during 5-part
counting cycle
2 RBC chamber draining
3 Vacuum cleaner
4 Waste chamber draining
5 Hb reference solution valve
6 Lysing solution valve
7 Diluent / Hb-Ref. solution valve for
peristaltic pump
8 Detergent solution valve
9 Peristaltic pump
10 Diluent solution valve
11 Interior / Exterior sampling probe
valve
12 Air / Vacuum in WBC chamber
13 Pump pressure valve
14 Pump vacuum valve
15 Predilution transfer valve
16 WBC chamber draining
17 Dispatches dilution in Hb
photometer or directly to waste
chamber.
19 Eo chamber draining
20 Eo lysing solution valve
21 Pressure sensor valve
22 Aspiration of detergent
Aspiration of dilutions during 3-part
counting cycle



ELECTRONIC DETAILS

BOARDS IMPLANTATION
The implantation of boards in the MS9-5 is
defined as follows :
Empty,
CPU Industrial Mother board,
Empty,
FK1-E = Fluidic board,
Empty,
CK1-E = Acquisition board,
Empty,
CNT9-E Sup = Upper Connection board,
CNT9-E Inf = Lower Connection board,
PAB9-E = Amplifier board.
Notes :



AMPLIFIER BOARD
External view
The amplifier board PAB9-E has a metallic box Notes :
protection against electrical noise.
The external description of this board shows :
The RBC/Plt measuring chamber probe,
The WBC measuring chamber probe,
The Eo measuring chamber probe,
The Hb photometer connectors,
The connector to the acquisition board,
The electric calibration access :
OE : Offset Eosinos
GE : Gain Eosinos
SE : Probe voltage Eosinos
OB : Offset WBC
GB : Gain WBC
SB : Probe voltage WBC
OR : Offset RBC
GR : Gain RBC
OP : Offset Platelets
GP : Gain Platelets
SPR : Probe voltage RBC/Platelets
Electric calibration must be done only by

authorized persons.



AMPLIFIER BOARD
Internal view
The internal part of the amplifier board PAB9-E Hb photometer :
board shows: receptor
emitter.
Measuring probes power supply,
Eo measuring probe voltage,
WBC measuring probe voltage,
Eo negative limiter, Notes :
WBC negative limiter,
Plt/RBC negative limiter,
Output Eo,
Output WBC,
Plt/RBC measuring probe voltage,
Output Platelets,
Output RBC,
Plt/RBC preamplification,
Plt/RBC probe,
WBC preamplification,
WBC probe,
Eo preamplification,
Eo probe,


CONNECTION BOARDS
There are two connection boards which are Notes :
called CNT9-E Sup and CNT9-E Inf.
The description of the connection boards shows:
Syringe stepper motor connector,
Up/Down stepper motor connector,
Rotation stepper motor connector,
Current limitation for motors,
The connectors from FK1-E :

Motors commands
Valves commands
The solenoid valve plugs,
The peristaltic pump connector,
The lysing reagent sensor connector,
Plugs to connect the lower card to the upper

card,
Blood tube detection switch connector,
The 25V input and pump stage :

power plug
driver
The reagent level sensor block connector.

CNT9-E Sup (Upper card)

CNT9-E Inf (Lower card)


FK1-E FLUIDIC BOARD

The description of the fluidic board FK1-E The motor drivers :
shows :
Rotation
Syringe
The motors, pump, reagent level sensors
Up/Down
connector (go to upper connection board),
Peristaltic pump.
The solenoid valves drivers :
Notes :
Valve 1 to valve 8
Valve 9 to valve 16
Valve 17 to valve 24
The solenoid valves connector (go to upper

connection board),
The pressure sensors calibration potentiometers,
Never touch gain G1 and G2.

Z1 and Z2 adjustment must be done
only by authorized persons.
The pressure sensors CPR1 () and

CPR2 ()
Only CPR1 is used (CPR2 is reserved

for future use).
The LED operational control,
The MS-Card reader,
The ISA bus controller,
The micro-controller and program storage,
The reagents level sensors,



CK1-E ACQUISITION BOARD

The description of the acquisition board CK1-E
shows :
Hb calibration (factory set),
Adjustments must be done only by

authorized persons.
The Hb acquisition unit,
Inputs (Plt/RBC/WBC/Eo) from amplifier

board PAB9-E and command signals +
power to PAB9-E,
The data storage component and CK1-E

program,
The LED operational control,
The ISA bus controller,
The micro-controller,
The digital acquisition unit,
The analog data conversion.
Notes :



MOTHER BOARD
The description of the mother board shows : Notes :
Power for CD-R/RW drive,
LCD TTL buffer,
2 USB ports,
Parallel printer port,
Serial port COM2,
External VGA monitor connector,
Ethernet connector (10/100 Mb/s),
Serial port COM1,
Internal keyboard connector (to passive

bus),
SODIM RAM,
The microdrive hard disk,
The microdrive connector,
The IDE bus connector for CD-R/RW drive.
Processor Intel MMX 266MHz low power,

The VGA controller,
RTC/Calendar, backup by industrial lithium
battery 3V/300mA,
The buzzer.



LCD TTL BUFFER

The description of the LCD TTL buffer shows :
Input connector to mother board,
Output connector for LCD cable,
Never used.
Don't confuse with keyboard extension
cable connector.
Notes :


COLOR LCD SCREEN CABLE

LCD SCREEN CABLE ADAPTER

POWER SUPPLY
The description of the Power supply shows :
(SK4) The high voltage entrance.

The standard voltage which could be
used is 100 to 240 Volts +/- 10%.
Frequencies 47Hz to 63Hz can be
used.
L is defined as : Line
N is defined as: Neutral
(SK2) The +12V / -12V / +25V output

for power supply.
(SK3) The +5V output.
Notes :
See: "Very important" information

page 2.

N L
220V
110V
DO NOT ADJ
1
+12V 1 è
+12V 3 è L
0V SK2 5 è N
0V
SK4
0V
-12V
PG
+25V
9
0V
1
0V SK3
2
+5V

ELECTRIC DIAGRAM
The description of the electric diagram Notes :
shows :
The +25V for drivers on FK1-E comes
The power supply : directly from the CNT9-E board.
+5V = Red
+12V = Yellow
-12V = Blue
+25V = Purple
Power Good (PG) = Orange
0V = Black
The power supply fan

The main fan
Connector CN1 on passive bus,
The main power filter :

Phases = Brown
Neutral = Blue
Ground = Green/Yellow
The passive bus power supply (PW1),
J10 connector of the lower connection

board (CNT9-E Inf) :
to the power supply
to the pump
The pump,
The mother board,
The CD-R/RW power supply.

1
+12V 1 è
+12V 3 è L
0V SK2 5 è N
0V
SK4
0V
-12V

PG
+25V
0V

+12V
0V SK3
0 V
+5V
1
+5V

-5V
-12V
+12V
+12V

0 V
0V
L N

1 +25V
PG 2 0V Red
1
+5 V
2 3 +24V +
+12V 4 0V
3 0 V
-12V Black
4
+0 V
5
+0 V
6
0V
1
0V

11
1234567
2
NC

12 11 12
+12V
12 12
3
0V
0V
12 11 12
+5V
12 12
+5V 12
12
11 12
12
4 12
12111
12
12
+5V
5 1 2 3 4
+5V
6

GROUND DIAGRAM
The description of the ground diagram shows :
The Main power filter connected with ,
The multi-voltage power supply,
The Main Ground Point on the metallic

base of the back side of the electronic part,
The LCD screen cable ground,
The upper metallic cover,
The front side fluidic part,
The upper connection board (CNT9-E

Sup),
The lower connection board (CNT9-E Inf).
Notes :

1
+12V 1 è
+12V 3 è L
0V SK2 5 è N
0V
SK4
0V

-12V
PG
+25V

0V
0V SK3
L N
+5V
1


GENERAL ELECTRONIC
DIAGRAM
See next page
Notes :



CELL MEASUREMENT

PRINCIPLE
The measuring principle of MS9-5 is the
impendance measurement.
Mechanical structure :
The measuring chamber consists of 2 parts. One
part is to receive the dilution which includes
the blood cells for analyzing and counting. The
other part, filled with detergent, is the aspiration
part in which a vacuum is applied . The
blood cell dilution of the first part crosses by
aspiration a calibrated aperture (Ruby) which
separates both parts.
Electrical structure :
2 electrodes are placed one in each part of
the measuring chamber. A constant current
is established between the two electrodes
across the aperture.
Phenomena :
When a cell, coming from the whole blood
dilution part, passes by a simple mechanical
aspiration, to the aspiration part of the
measuring chamber, it crosses the aperture .
Because of its different resistivity compared to
diluent, the cell disturbs the constant current
established between the two electrodes and
generates a pulse .
Notes :


ñ

ñ ñ ñ


PROBE VOLTAGE
CALIBRATION
The current established in the measuring Press the 'Calibration' button to run the
chamber depends on the voltage applied to the cycle. When finished, new values () will
electrodes of the probe. This voltage is called appear below the old ones. Verify that the
'Probe voltage' and has to be calibrated when new values are in the range below :
replacing : _ 150 < RBC calibration voltage < 170
_ aperture _ 170 < WBC calibration voltage < 190
_ measuring lateral chamber _ 140 < Eosino calibration voltage < 160
_ measuring chamber and WBC calibration voltage is greater than
_ amplifier board PAB9-E RBC calibration voltage.
_ acquisition board CK1-E Click 'Save' button to store the new
values and uptade them in the 'Sensitivity'
Probe voltage setting : dialog () (menu 'Settings', 'System').
The 'Probe voltage' dialog () (menu Never modify these values inside this dialog.
'Settings', 'Bank', 'Edit') shows one slide per
probe with which you can change the actual
probe voltage.
Probe voltage settings can be modified only

by authorized persons.
Probe voltage calibration cycle : Notes :

The calibration cycle is available in the
'Probe voltage calibration' dialog () (menu
'Calibration', 'Probe voltage'). This dialog shows
the current values for amplifier calibration voltage
and spoiling delta of aperture (: Old values).
It is necessary to perfectly clean all the
apertures before running the calibration cycle.
To clean them use the technical functions 'RBC
aperture cleaning', 'WBC aperture cleaning' and
'Eosino aperture cleaning' (menu 'Service',
'Technical functions', F6). You must also check
that all reagents have been primed before. If
not, you can prime them with the technical
function 'Complete priming' (F6).



PULSE AMPLIFICATION
The electronic pulse amplification principle of Notes :
MS9-5 is described as follows :
Components :
Plt/RBC chamber with its probe (example)

Probe voltage feedback control
Probe voltage command
Pulse preamplification
Pulse amplification
Negative limiter
Plt pulse calibrated amplification (last
stage)
RBC pulse calibrated amplification (last
stage)
Same for WBC
Same for Eo
Current / Voltage converters
Hb amplification
Hb photometer
Actions :
Probe voltage calibration

Probe voltage feedback control
Probe voltage command
Gain potentiometer
Offset potentiometer
Plt signal to CK1-E acquisition board
RBC signal to CK1-E acquisition board
WBC signal to CK1-E acquisition board
Eo signal to CK1-E acquisition board
Hb signal
Emitter command


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PULSE ACQUISITION
The electronic pulse acquisition principle of the Notes :
MS9-5 is described as follows :
Components :
RBC pulse inverter/filter (from amplification

output) (same for Plt and Eo),
DAC attenuator,
Inverter,
8 bits flash ADC,
WBC pulse inverter/filter,
DAC attenuator,
Inverter,
8 bits flash ADC,
PowerCell (digital acquisition unit),
Controller,
Reference Hb,
Comparator,
DAC,
Hb voltage amplification.
Actions :
Probes voltage input

Reference voltage generator
Hb calibration
Plt/RBC probe voltage
WBC probe voltage
Eo probe voltage
Hb emitter
Hb signal

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PULSE DIMENSIONS
The PowerCell included in the electronic
schematic architecture of the acquisition board
gives a cell by cell 4 dimensional study for each
of the blood population.
Dimensions :
The artificial volume of the pulse, or

the greatest slide of the pulse
crossing the aperture.
The real volume of the pulse,
The crossing time,
The intermediate point of the pulse.
Notes :


ELECTRONIC FILTERS
The electronic pulse filters are programmed in
the bank setup threshold dialog of the MS9-5
software (Menu 'Services', 'Bank', 'Edit',
'Threshold'). They are available for the 4
measuring channels (Plt, RBC, WBC, Eo). The
actions as pulse filters for each of the following
filter parameters are defined as follows :
Minimum height = Low threshold

Maximum height = High threshold
Minimum width = Short time window
Maximum width = Large time window
Changing the filter parameters can only be

done by authorized persons.
Notes :



HISTOGRAMS
The heights of the electric pulses which
are generated by the blood cells are converted
by a 8 bits ADC convertor . The electric pulses
are analyzed by the PowerCell which checks
the validity of the cells. Then the heights are
and stored in the
treated by the controller
data storage chip (RAM) under a 255
channels memory area . The volumetric
correspondence of this area is incremented
each time that a cell is accepted and stored
(). This memory area creates a volumetric
distribution histograms.
Each blood population Plt, RBC, WBC, Eo

has its own volumetric distribution histogram.
The morphology of the normal Plt histogram is
LOGNORMAL , the normal RBC histogram
is NORMAL and the under population (LMG)
volumetric distributions of the WBC are
NORMAL . As an under population of WBC,
the normal Eo histogram is also NORMAL.
Notes :


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HISTOGRAM SMOOTHING
The volumetric histograms generated by the
height of the pulses have original rough structures
which are slightly variable. To stabilize the
structure of the histograms a smoothing software
process is needed. This software smoothing
process can be defined and adapted with the
data of the bank setup smoothing dialog of the
MS9-5 program (Menu 'Services', 'Bank', 'Edit',
'Smoothing').
For smoothing the histograms 2 parameters are

used :
Smoothing count
= Number of smoothing cycles for
calculations (computing)
Smoothing matrix size
= Number of smoothing elements.
Changing the smoothing parameters for

calculation can only be done by authorized
persons.
Smoothing count
= Number of smoothing cycles for
display
Smoothing matrix size
= Number of smoothing elements.
Notes :

Smoothed for calc.
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Smoothed for disp.
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Hb PHOTOMETER PRINCIPLE
The photometer measures the Hemoglobin (Hb)
value with the total reduced Hb hemolyzed blood
solution which comes from the first dilution of
the second sample previously analyzed and
counted in the WBC chamber.
A reference value for Hb (Blank Hb) from the
Hemoref® solution is needed and is measured
automatically at the counting cycle start-up.
Hb br = Hb blood result
Hb bk = Hb reference value
Hb dil = Hb WBC dilution value
Þ Hb br = Hb dil - Hb bk
The measuring principle is a Beer-Lambert

photometry integration which is done with a
capacity / resistivity system. The Beer-Lambert
reaction gives an exponential result of the
concentration which is linearly integrated by
the inverse function, done by a similar
capacity / resistivity integrator.
The main weakness of a photometry sensor is
its very high variation in temperature. This is the
reason why a reference value is needed. Only
the difference in value between Hb dil and
Hb bk is fixed even if the temperature shifts.
Notes :

Time
Hb bk
Hb dil
Hb br


DILUTION

PRINCIPLE
The dilution principle is a sequential dilution made
of 2 samples and 3 successive dilutions.
First sample :
The first dilution is prepared in the Eo chamber.
Half part of the needed Eo lysing reagent is
poured into the Eo measuring chamber . The
needle carries some blood directly from the
whole blood tube in the Eo chamber (with a
few quantity of diluent). Then the second half of
Eo lysing reagent quantity is poured.
Second sample :
At the beginning of the second dilution a small
quantity of diluent is poured in the predilution
cuvette . Then the needle brings another
whole blood sample in the predilution cuvette
and some more diluent is added. Then the
needle samples a little bit of this prediluted
blood.
At this step, the blood is carried to the WBC

measuring chamber with some lysing reagent
via the buffer tube . It will be used to count
WBC and to measure Hb.
The blood left in the needle is diluted once more

in the RBC measuring chamber . It is used to
carry out the Plt/RBC measurement.
Notes :

1st sample : Dilution Eo (5-Parts only)

2nd sample : Dilution WBC/Hb & Plt/RBC
Lysing reagent

Air
vacuum


DILUTION CYCLE DATA

The dilution is carried out in several stages which Notes :
are defined by programmed data. The
programmed data is available and can be
modified for specific adaptation with the MS9-5
program (menu 'Settings', 'Fluidic').
There are 5 dilution configuration tabs, each
containing specific parameters as follows:
Syringe stepper motor

Up/Down stepper motor used to drive up
and down the needle
Rotation stepper motor used to drive left
and right the needle
Structure of bubbles :
for mixing Eo Dil. in 5-part mode
for mixing RBC/Plt Dil. in 3-part mode
Vacuum control parameters
Counting vacuum = speed at which the
prediluted blood will be sucked during
counting
Dilution vacuum transfer = speed at which
the prediluted blood will be sucked to the
WBC measuring chamber from the PDL
cuvette
Hb priming vacuum = speed at which the
diluted blood will be sucked to the Hb
photometer
Sensors ON/OFF & Sensitivity
Priming, transfer and emptying specified
times
Changing the dilution parameters can only

be done by authorized persons.


.../...

DILUTION CYCLE STEPS

The description of the dilution cycle has the following
conventions :
1) The data which is used in the dilution cycle is

written in italic. This data is available in the dilutor setup
dialog (F12) of the MS9-5 program.
2) The Delay data is defined in a time base of 50

milliseconds (there are some exceptions).
3) The motor movements are mentioned in real motor

steps. The motor maximum speed is 1 (one) step every
4.4 millisec. for Up/Down motor, 1 (one) step every 2.2
millisec. for rotation, and 1 step every 8.8 millisec. for
the syringe stepper motor. The motor speed data is
defined in 2.2 millisec. per step.
4) Every rotation movement of the sampling probe

(Needle) are carried out with a speed equal to Rotation
speed. The rotation distances between the sampling
probe actions are defined in real steps.
5) The height (Up/Down motor) of the sampling probe

in the different cuvettes is defined in real steps
6) Section Others of the Dilutor setup dialog : The

vacuums or pressure data are programmed in ADC units.
To convert it in mBar, calculate as follows : Pressure =
125 - (2.45 * Y) where Y is in ADC unit and Pressure
in mBar.
7) In the following description the RBC chamber will

be called RBC, the WBC chamber will be called WBC,
the Eo chamber will be called Eoc and the predilution
cuvette will be called PDL. All cuvettes and chambers
are draining into the waste chamber but not the predilution
cuvette which is draining into the WBC chamber.
The needle cleaning system is composed of the Needle
cleaning Head, valve 3 and the peristaltic pump.

DILUTION CYCLE STEPS .../...

When a whole blood tube is positioned in the whole
blood carrier a switch under it is activated. This switch
is controlled by the main software. The MS9-5 sends
the order to start a dilution cycle to the FK1-E board.
0) The main software sends the four following data to
the FK1-E :
- Blood quantity to sample for Eo
- Lysing quantity to prime in Eo
- Blood quantity to sample for WBC/RBC/Plt
- Lysing quantity to prime in WBC
1) The FK1-E tests the reagent levels in the following
order :
- Lyse
- Lyse Eo
- Diluent
- Detergent
- ref. Hb
If one is missing the cycle stops and an error is
displayed on the screen.
2) The FK1-E pulls the syringe of Pitch setting (Cal_T)
to block the mechanical variations of the blood sampling
system + Diluent quantity rinsing #1 (QtRinc1) steps. At
the same time, the sampling probe goes to the whole
blood carrier by moving of Distance PDL->blood tube
holder steps. The down speed of the sampling probe is
defined by Sampling speed.
During the whole blood sampling action the FK1-E starts

several time sharing actions to waste the cuvettes and
chamber and to prime the reagents. They are defined as
follows :
a) The compressor starts with valve 14
switched on to create vacuum in the waste chamber.
b) The valve 19 is activated during 1 sec. to
empty the Eoc by aspiration.
c) The valve 16 is activated during Delay #2
for draining WBC chamber x0.05 sec. to empty the
WBC by aspiration.
d) The RBC chamber is then emptied by means
of the valve 2 during Delay #2 for draining RBC chamber
x0.05 sec.
e) Valves 5 and 17 are activated during Delay
for hemoref priming x0.05 sec. to fill the photometer
with Hb ref. solution.
f) The compressor is then switched off with
valve 14 off. The valve 13 is activated and the compressor
creates pressure in the waste chamber.
g) Valve 1 is activated during Delay for cleaning
apertures x0.05 sec. to back flush the apertures.
h) Valve 4 is activated during Delay for draining
waste chamber x0.05 sec. for draining the waste chamber.
i) The valves 1, 4 and 13 is switched off and
14 on during 3 sec. to reset the waste chamber to the
atmospheric pressure.


j) Valve 2 is then activated during 1 sec. to
assure a perfect wasting state of the RBC chamber
which contains detergent in at this step.
k) Valve 16 is then activated during 1 sec. to
assure a perfect wasting state of the WBC chamber
which contains detergent in at this step.
l) Then the waste chamber goes under Max
vacuum.
During the above stage the fluidic steps to operate the

dilution for Eosinos, are as follows :
3) When the sampling probe is in the whole blood
sampling tube the FK1-E waits 0.5 sec. The syringe is
pulled of Blood quantity for eosinos (QtSang1b) steps to
sample the whole blood at the speed sampling speed.
4) The FK1-E waits 0.5 sec. and the sampling probe
goes up by 200 steps with the "needle cleaning system"
ON. The sampling probe speed up at this step is Speed
with blood.
5) The syringe pump pulls QtCompLysEo/2 steps with
the following calculation :
QtCompLysEo = <QtLyseEo>-<QtSang1b>-<QtRinc1>+<Cal_T>
to protect the sampled blood in the sampling probe.
6) The sampling probe goes to the Eoc Distance
blood tube holder->Eo chamber and the syringe pump
pulls again with QtCompLysEo/2 steps. Then the "needle
cleaning system" is switched off.
7) The FK1-E pushes the syringe of Pitch setting
(Cal_T) to block the mechanical variations of the blood
sampling system.
8) Valve 20 is activated and the syringe is pushed to
the step quantity (Lyse eosinos quantity)/2 (QtLyseEo/2)
9) Valve 10 and 11 are activated, the syringe pump
is pushed of (QtRinc1/2) steps to push blood with diluent.
10) Valve 11 is closed, the syringe pump is pushed of
(Diluent quantity rinsing #1)/2 (QtRinc1/2) steps again to
clean external part of the sampling probe with diluent.
11) Valve 10 is closed and the syringe is pushed to
the step quantity (Lyse eosinos quantity)/2 (QtLyseEo/2) to
do the last part of the Eo dilution. Then valve 20 is
closed and the syringe goes back to its home position.
Then the fluidic does the second sampling and so the

fluidic steps to operate the first dilution, for WBC/
RBC/Plt, are as follows :
12) The FK1-E pulls the syringe of Pitch setting (Cal_T)
steps to block the mechanical variations of the blood
sampling system. At the same time, the sampling probe
goes to the whole blood carrier by moving of Distance
PDL->blood tube holder steps. The down speed of the
sampling probe is defined by Sampling speed.


13) When the sampling probe is in the whole blood
sampling tube the FK1-E waits 0.5 sec. The syringe is
pulled of Blood quantity (QtSang1) steps to sample the
whole blood at the speed sampling speed.
14) The FK1-E waits 0.5 sec. and the sampling probe
goes up by 200 steps with the "needle cleaning system"
on. The sampling probe speed at this step is Speed with
blood.
15) The syringe pump pulls the Lyse quantity (QtLyse)
steps to protect the sampled blood in the sampling probe.
16) The sampling probe goes to the PDL Distance
blood tube holder->PDL and the syringe pump pulls
Pitch setting (Cal_T) add with First diluent quantity for
WBC dilution (QtDil1A) steps. Then the "needle cleaning
system" is switched off.
sampling system.
18) Valve 6 is activated and the syringe is pushed of
the step quantity Lyse quantity (QtLyse).
19) The first dilution is carried out in the predilution
cuvette as follows (with valve 6 always on) :
- Valve 10 on and the syringe pump is pushed
with First diluent quantity for WBC dilution steps with a
speed Speed for first part of dilution.
- The sampling probe is turned to the left with
Rotation for WBC dilution steps.
- Valve 11 is activated and the syringe pump is
pushed by Second diluent quantity for WBC dilution
steps with a speed Speed for second part of dilution.
- The sampling probe rises Height for WBC dilution
steps. The syringe is then pushed Third diluent quantity
for WBC dilution steps with a speed Speed for third part
of dilution.
- Wait for Delay for dilution stabilization x0.05
sec. then valves 10 and 11 are switched off.
20) Then valve 6 is closed and the syringe goes back
to its home position. The sampling probe does a 'drop-
off' over the PDL cuvette.
The fluidic steps to operate the second dilution, for

WBC/RBC/Plt, are as follows :
21) The sampling probe is turned to the right with
Rotation for WBC dilution steps and the syringe is pulled
of Pitch setting (Cal_T) steps to block the mechanical
variations of the blood sampling system.
22) The sampling probe goes down of 55 steps in the
PDL cuvette.
23) The FK1-E waits 0.5 sec. Then the syringe is
pulled of Blood quantity for RBC/PLT dilution steps to
sample the whole blood at the speed sampling speed.
24) Wait for 0.75 sec. then the sampling probe rises
by 55 steps.


25) The syringe pump pulls Lyse quantity (QtLyse)
steps to protect the sampled blood in the sampling probe.
26) The sampling probe goes to the RBC cuvette with
Distance PDL->RBC chamber steps, the syringe pump
pulls Diluent add quantity for RBC/PLT add with Diluent
quantity rinsing #2 steps, and then wait the end of the
previous task which started at step 2 of this dilution
description.
sampling system. Valve 6 is switched on and the syringe
pump pushes Lyse quantity steps.
28) Valve 10 is activated and the syringe pump goes
up Diluent quantity rinsing #2 steps with a speed Speed
up to clean the outside of the sampling probe over the
RBC cuvette and for decontaminating the RBC chamber.
The lysing reagent is then checked in the buffer tube
arriving in the WBC chamber with the sensitivity defined
by sensor sensor for Lyse level.
29) Valves 6 and 10 are switched off. Valve 16 is
activated to waste perfectly the WBC chamber (after the
back flush process) during Delay #2 for draining WBC
chamber x0.05 sec.
30) Valve 2 is activated during Delay #2 for draining
RBC chamber x0.05 sec. to empty the RBC chamber.
31) A WBC dilution vacuum transfer is established in
the waste chamber and the second dilution is carried
out :
- Valve 10 on and the syringe pump is pushed
with First diluent quantity for RBC/PLT dilution steps
with a speed Speed for first part of dilution.
- The sampling probe is turned to the left with
Rotation for RBC/PLT dilution steps.
- Valve 11 is activated and the syringe pump is
pushed by Second diluent quantity for RBC/PLT dilution
steps with a speed Speed for second part of dilution.
- The sampling probe rises Height for RBC/PLT
dilution steps. The syringe is then pushed Third diluent
quantity for RBC/PLT dilution steps with a speed Speed
for third part of dilution.
- Wait for Delay for dilution stabilization x0.05
sec. then valves 10 and 11 are switched off.
32) At the same time, the PDL transfer starts by
activating valves 1, 12, 15. The transfer sensor is checked
during this transfer.
33) The sampling probe does a 'drop-off' over the RBC
cuvette.
34) The waste chamber is under vacuum and the
Detergent is primed during 2nd delay for transflux priming
x0.05 sec. The sampling probe goes to PDL with a
rotation of Distance RBC chamber->PDL steps. The
detergent sensor is checked during all the priming
sequence with a sensitivity of sensor for detergent level.


35) Valve 1 is switched off and the main vacuum
chamber goes in pressure as defined by Bubble pressure.
36) A few mixing bubbles are carried out into the Eo
chamber with the following sequence :
- Valve 2 is switched on/off Bubble quantity times
- during an activated cycle of :
Bubble size*0,0011 sec.
- each bubble is separated within 0.25 sec.
37) The waste chamber is then set to counting vacuum.
The syringe pump position is reset by means of valve 10.
38) Valve 1 is activated and the sampling probe does
a 'drop-off' over the PDL cuvette.
39) A test is made to reach the counting vacuum and
all the motors are switched off.
[...] COUNTING CYCLE
When the counting cycle is terminated the mother board

sends an order to the FK1-E to prime the photometer
with the WBC dilution :
40) Valve 8 is activated during 2 sec. to quickly clean
the lateral chambers. At the same time the sampling
probe goes to Eoc with a rotation of Distance PDL->Eo
chamber steps and the syringe pulls 200 steps.
41) Valve 1 is closed and valve 13 is activated 0.5 sec.
to decrease vacuum inside the waste chamber.
42) Then the waste chamber is calibrated with a
vacuum of Hb priming vacuum.
43) Valves 16 and 17 and 19 are activated and wait for
Delay for prepare Hb Priming x 0.05 sec.
44) Valve 19 is closed and valve 2 is opened during
Delay for Hb Photometer chamber x 0.05 sec. to begin
to empty the RBC cuvette while the Hb photometer is
being primed. At the same time Valve 10 is activated
and the syringe pump goes up to its home position to
rinse Eoc with diluent.
45) Valves 2 and 17 are closed, valve 19 is opened
and a wait state of 1 sec. is made to allow the blood
dilution in the photometer to stabilize.
46) Valve 16 and 19 are closed and the main software
measures the Hb and the vacuum is still maintained for
1.5 sec. Then valves 12 and 15 are activated to begin the
emptying of the PDL cuvette during 1.5 sec.
47) Valves 12, 15 are activated during delay for draining
PDL x0.05 sec. to finish the PDL emptying.
48) Valve 19 is activated during 1 sec. to begin the
emptying of the Eoc chamber.


49) Valve 5 and 17 are opened during 2 sec. to wash
Hb photometer and the sampling probe goes back to
PDL with a rotation of Distance Eo chamber->PDL steps.
At this stage of the dilution the MS9-5 main software

checks if a new whole blood tube is programmed in the
tube holder. If it is the case the MS9-5 orders to the
FK1-E board to start a new cycle (go to step 1).
In the other case :
50) The reagent levels are checked and an error is
displayed in case one is missing. The main vacuum
chamber is still under vacuum and valves 5 and 17 for 1.5
sec. to rinse the photometer.
51) Valve 16 is activated during 2.5 sec. to perfect the
waste of the WBC chamber.
52) The sampling probe goes up to its home position
and valve 3 is activated to dry the probe.
53) The sampling probe goes down by Height inside
the PDL steps and then valve 3 is closed. At the same
time Eo chamber is emptying, valve 19 is activated during
2x Delay #1 for draining WBC chamber x0.05 sec.
54) Valve 2 is activated during 2x Delay #1 for draining
RBC chamber x0.05 sec.
55) The peristaltic pump turns in the reverse way during
0.5 sec. to decrease the pull of the pump tubes.
56) The vacuum is stopped with valve 14 off and the
waste chamber is set at the atmospheric pressure. Then
all valves are switched off.


COUNTING

PRINCIPLE
The MS9-5 counting cycle is structured in 4 main The Hb measurement :
parts : The Hb measurement is carried out
at the end of the counting cycle. The Hb
The voltage setup and Hb Blank : number of measurement cycles is defined in
This cycle takes a few seconds for the the MS9-5 system setup dialog (menu
Hb blank measurement and the probe voltage 'Settings', 'System', 'Hb', 'Number of
set and control . The Hb number of blank measuring cycles').
measurement cycles is defined in the MS9-5
system setup dialog (menu 'Settings', 'System',
'Hb', 'Number of reference cycles'). Notes :
The cell counting and clot control :

Every 150 millisec. during the counting
cycle the digital probe voltage are checked with
the CVDC® concept. This MS-Labs patent
concept gives a very efficient and accurate real
time clot control.
The counting cycle is partitioned in 4 digital
counting data blocks ,,,. Each block
stores the following data:
- Real time length of the block
- Quantity of cells counted
- MCV of the block
The blocks are compared to each other.
A delta (%), corresponding to the difference
between the cells counted in each block is
calculated (∆1, ∆2, ∆3). This delta activates
an alarm if the value goes over the user
programmed value (menu 'Settings', 'System',
'Sensitivity', 'Counting delta for clogging').
The histograms acquisition.

This part is performed simultaneously with
part .


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Counting time

COUNTING CYCLE DATA

The MS9-5 counting data is divided into 7 parts : The probe voltage during
counting :
The Hb measurement data : RBC/Plt probe voltage in 1/10 V
Number of reading cycles for Hb WBC probe voltage in 1/10 V
measurement. Eo probe voltage in 1/10 V
Number of reading cycles for Hb
reference measurement.
Mini/Maxi LED current for Hb reference
measurement. Changing the parameters
The Clot sensitivity detection : can only be done by authorized
Acceptable range of voltage = acceptable
persons.
range in which the voltage is set before
the beginning of the counting.
Voltage delta for clogging = acceptable
voltage range during the RBC/Plt, WBC,
Notes :
Eo counting cycle.
Counting delta for clogging = acceptable
delta between the Plt, RBC, WBC, Eo The amplifier calibration voltage and the
counting blocks. spoiling delta of aperture values have to be
Acceptable delta for counting vacuum =
acceptable delta between the starting set very rarely. To set them, you must run a
vacuum and the ending vacuum. calibration cycle (see 'Calibration cycle'
RBC/WBC/Eo amplifier calibration voltage = section)
calibration of the basic current field in the
corresponding chamber.
Spoiling delta of RBC/WBC/Eo aperture =
acceptable range of calibration for the
basic current field.
The reference counting data :
Reference counting time value.
Reference for RBC channel gain (for MCV
measurement).
Reference counting vacuum
1st dilution reference rate (WBC dilution)
2nd dilution reference rate (RBC/Plt
dilution)
Reference probe voltage
The counting and Histograms cycle
times :
Cell counting time in seconds (one per
channel).





RESULTS

RESULT MEASUREMENT
The MS9-5 proceeds with the following
parameter measurements as follows :
1) Plt, RBC, WBC, Eo :

The MS9-5 counts a number of cells from
a specified dilution during a fixed time (normal
counting),
or
counts the time until a fixed quantity of cells
is reached (proportional counting).
2) Hemoglobin :
The MS9-5 measures the hemoglobin
under its reduced form Cyanmethemoglobin.
The measurement is taken from the first dilution
at 540 nm (DRAPKINS method).
A blank reference measurement is taken
during each analysis on the reference solution
'Hemoref®'.
3) MCV :
The MS9-5 measures the MCV (), then
to obtain the Hematocrit result the following
calculation is done :
Hematocrit = MCV * RBC
4) MPV :
The MS9-5 measures the MPV (), then
to obtain the Plateletcrit result the following
calculation is done :
Plateletcrit = MPV * Plt
Notes :

MCV
qty

fl
MPV
qty

fl

EOSINO CALIBRATION
The MS9-5 proceeds at the following parameter
measurements as follows :
The Eo volumetric histogram () shows a

hollow with stromas on the left and eosinophil
cells on the right. The histogram is clipped so
that only the eosinophils part is kept.
The clipping volume is the minimum height

for Eo, which can be set in the 'Threshold'
configuration dialog (menu 'System', 'Bank',
'Edit', 'Threshold'). The usual value for this
threshold is between 30 and 40.
Notes :

Minimum
height
qty
fl
Stromas Eosinophils
123
1234567890123456789012345678901212345678901234567
1234567890123456789012345678901212345678901234567
123
1234567890123456789012345678901212345678901234567
123
0 è 255

RESULT CORRECTIONS
The MS9-5 software can correct the result from Notes :
many kinds of natural effects. It corrects the Log
lost in very high result cases. With the smoothing
routines, it pulls down the low results.
Log/Log amplification corrections :

The correction array, in the 'Bank correction
setup' dialog (F5) does an exponential
compensation of the Log. lost in the very high
results. The 3 channels which are very sensitive
for such compensation are the Plt, WBC and
Eo channels. The usual correction values is
0.030 for Plt, 0.012 for WBC and 0.060 for Eo.
The smoothing pulling down

corrections :
The effect of such smoothing calculation
routines is to moderate the volumetric cell
distribution morphology. It eliminates the hazard
of the wrong population. The effect is to pull down
the result. The 2 channels which are very
sensitive for such compensation are the Plt, and
Eo channels.
Examples :
High smoothing
Smoothing count = 9
Smoothing matrix size = 5
Normal smoothing
Smoothing count = 6
Smoothing matrix size = 5
No smoothing
Smoothing count = 0

õõ

ò
ò
ò

RESULT CALCULATIONS
The MS9-5 does the following calculations to
deliver the results :
1) Plt, RBC, WBC : 4) Hct :

The software uses the following The software uses the following
parameters to do the calculations : parameters to do the calculations :
- Number of cells counted - Measured MCV value
- Time of counting cycle - Factory MCV calibration value
- Factory calibration value - User MCV calibration value
- User calibration value - Blood Bank MCV calibration value
- Blood Bank calibration value - Exponential compensation
- Smoothing ratio Off - Gain and probe voltage
- Exponential compensation compensation
- Blood dilution compensation
5) Pct :
2) Eo : The software uses the following
The software uses the following parameters to do the calculations :
parameters to do the calculations : - Measured MPV value
- Number of cells counted - Factory MPV calibration value
- Time of counting cycle - User MPV calibration value
- Factory calibration value - Blood Bank MPV calibration value
- User calibration value - Exponential compensation
- Blood Bank calibration value - Gain and probe voltage
- Smoothing ratio Off compensation
- Exponential compensation
Eo dilution compensation
-
WBC dilution Notes :
3) Hb :
The software uses the following
parameters to do the calculations :
- Measured blank value
- Measured Hb Value
- Factory calibration value
- User calibration value
- Blood Bank calibration value
- Exponential compensation


ANALYTICAL PARAMETERS
The MS9-5 also delivers some analytical Notes :
parameters defined as follows :
1) Plt :
Pct : Plateletcrit
MdP : Platelet Mode
MnP : Platelet Median
µPlt : Micro Plt Ratio
MPlt : Macro Plt Ratio
PDW : Plt Distribution Width
(Standard Deviation)
2) RBC :
Hct : Hematocrit
MCH : Mean Cell Hb
= Hb / RBC
MCHC : Mean Cell Hb Concentration
= Hb / Hct
µRBC : Micro RBC Ratio
MRBC : Macro RBC Ratio
RDW-SD : Red cells Distribution Width
(Standard Deviation)
RDW-CV : RDW-SD / MCV
(Coefficient of Variation)
3) WBC
Lym% : Lymphocytes ratio (%)
Lym# : Lymphocytes absolute value
Mon% : Monocytes ratio (%)
Mon# : Monocytes absolute value
Neu% : Neutrophils ratio (%)
Neu# : Neutrophils absolute value
Bas% : Basophils ratio (%)
Bas# : Basophils absolute value
Eo% : Eosinophils ratio (%)
Eo# : Eosinophils absolute value

qty

fl
MPV = Same volume on
each side
qty
100%

fl
Mode = Most frequent volume
qty 50% 50%

fl
Median = Same number of cells
on each side
qty

ç è
fl
µPlt MPlt

Plt HISTOGRAM DEVIATION

The platelet histogram can have some erratic
deviations due to many reasons described as
follows :
Left side interference :

modify : Quantity increased
Quantity very unstable
Mode very low (sometimes)
MPV decreased
Due to : No/Bad ground (no earth)
Expired reagents
Contaminated reagents
Leak on chamber waste valve
Modal cut of the histogram with step

on 10 and step on 15 etc... :
modify : Quantity decreased
Macro Plt and MPV increased
Abnormal mode (sometimes)
due to : Micro-agglutinations which can
be confirmed on the WBC
histogram when the Plt are very
low and the WBC quantity is
increased.
Right side interference :

modify : Quantity very much decreased
Macro Plt and MPV increased
due to : Micro RBC
Notes :

qty
fl
5 10 15
qty
fl
5 10 15
qty
fl
5 10 15

3 PART-DIFF PARAMETERS
The MS9-5 carries out the 3 part differential Notes :
calculation on the Leukocyte population.
This calculation is carried out from fixed cursors
which are programmed in the Min/max
configuration dialog of the MS9-5 software (menu
'Services', 'Bank', 'Edit', 'Min/max cell volumes
ranges').
Start End
WBC area
Defined from to
Lymphocytes area
Defined from to
Monocytes area
Defined from to
Granulocytes area
Defined from to
Immature Cells area
Defined from to
Platelet clusters, Nucleated RBC
Defined from to
It is possible to set the cursors from to

in the Min/max configuration dialog. The 's
area of the dialog contains the parameters
specific to 3 part-diff for WBC.


123456789012345678901234567890121234567890123456789012345
123456789012345678901234567890121234567890123456789012345
123456789012345678901234567890121234567890123456789012345
0 100 200 300 400 fl
0 64 128 192 255


3/5 PART-DIFF PARAMETERS

The MS9-5 carries out the measurement
of Plt and RBC from fixed cursors which are
programmed in the Min/max configuration dialog
() of the MS9-5 software (menu 'Services',
'Bank', 'Edit', 'Min/max cell volumes ranges').
The Plt and RBC cursor locations are the
same wether the MS9-5 works in 3 or 5 part-
diff. . They are located in the '3 part-diff / 5 part-
diff' area ().
Plt histogram ()

µPlt area
Defined from 0 to 'Start'
Plt area
Defined from 'Start' to 'End'
MPlt area
Defined from 'End' to 255
RBC histogram ()

µRBC area
Defined from 0 to 'Start'
RBC area
Defined from 'Start' to 'End'
MRBC area
Defined from 'End' to 255
Notes :

Start End
qty

fl
123
12345678901234567890123456789012123456789
12345678901234567890123456789012123456789
123
123
12345678901234567890123456789012123456789
0 è 255
ç è
µPlt MPlt
Start End
qty

fl
12345678901234567890123456789012123456789
123
12345678901234567890123456789012123456789
123
12345678901234567890123456789012123456789
123
0 è 255
ç è
µRBC MRBC


5 PART-DIFF PARAMETERS
The MS9-5 carries out the 5 part differential Notes :
calculation on the Leukocyte population.
This calculation is carried out from fixed cursors

which are programmed in the Scattergram
configuration dialog () of the MS9-5 software.
To access this dialog, click on 'WBC' button ()
in the Min/max configuration dialog (menu
'Services', 'Bank', 'Edit', 'Min/max cell volumes
ranges', ).
The Scattergram configuration dialog shows 4
areas :
: Lymphocytes area
: Basophils area
: Granulocytes area
: Monocytes area
To modify a sensitivity, click in the corresponding

area ( to ).
For example, click in basophils area () :
Handles will appear ( p.162), which you can
drag to the requested sensitivity. You can
decrease basophils sensitivity by shortening the
Basophils area ( p.162).





ERROR CODES

FLUIDIC ERROR CODES

The MS9-5 fluidic error codes are described as
follows :
code Problem code Problem

1 Vacuum error 26 Aperture vacuum error during Eo
2 Transflux sensor error or transflux aperture cleaning
empty 27 Peristaltic pump error
3 Hemoref sensor error or hemoref
empty
4 Isoflux sensor error or isoflux empty
5 Predilution transfer error Notes :
6 Lyse sensor error or lyse empty
7 Predilution cuvette draining error
8 Vacuum needle cleaning head sensor
or peristaltic pump error
9 Waste chamber draining sensor error
10 Needle error
11 Syringe error
12 Needle + syringe error
13 Blood tube clotted
14 Transflux priming error during dilution
preparation
15 Counting vacuum error
16 Predilution transfer vacuum error
17 Lyse supply during lyse priming /
dilution preparation
18 Transflux priming vacuum error
19 Hemoref priming vacuum error
20 Aperture vacuum error during RBC
aperture cleaning
21 Aperture vacuum error during WBC
aperture cleaning
22 Atmosphere pressure error
23 bubble pressure error
24 Hb photometer priming vacuum error
25 lyse Eo sensor error or lyse Eo
empty

FK1-E ERROR CODES

The MS9-5 FK1-E error codes are described as
follows :
code Problem
201 Mode type badly transmitted
203 Wrong data transmitted
230 Fluidic time out
232 I²C time out
233 I²C bus busy
240 Bad code transmitted
241 Motor Busy
242 Whole blood tube capped
243 Optical X/Y row error
244 Up/Down detection error
901 FK1-E board busy
902 Motor error
905 Wrong answer, FK1-E board out of work
Notes :

CK1-E ERROR CODES

The MS9-5 CK1-E error codes are described
as follows :
code Problem
201 Mode type badly transmitted
202 Hardware error
203 Wrong measuring channel number
230 CK1-E board time out
240 Bad code transmitted
241 Unavailable function
242 Hb system non operational
900 Threshold error
901 Wrong parameter value
902 Out of memory
903 Calculation error
905 Wrong answer, CK1-E board out of
work
Notes :



REF. SPARE PARTS

COMPLETE PARTS
Reference MSL Description
3MS09 315 ∅60µm RBC aperture complete

3MS09 325 ∅80µm WBC aperture complete
3MS09 325 ∅80µm Eo aperture complete
3MS09 902 Whole blood tubes colored adapters (4 pcs)
3MS95 910 MS9-5 RBC & Eo measuring chambers complete
3MS95 911 Complete WBC measuring chamber block for MS9-5
3MS09 915 Main waste chamber complete
3MS95 920 MS9-5 syringe pump complete with motor
3MS95 921 MS9-5 syringe pump without motor
3MS95 930 X/Y system complete for MS9-5
3MS95 931 Sampling probe complete for MS9-5
3MS95 935 Needle cleaning head complete
3MS09 940 Photometer complete
3MS95 981 TFT color screen block complete (3MS09 281C + 3MS09 282C
+ connector + metallic part)

ELECTRONIC PARTS
0MSCPBUS Passive bus and slots

3MS04 153 Mini-switch for syringe
3MS95 210 FK1-E fluidic board
3MS95 215-i Lower connection board CNT9-E
3MS95 215-s Upper connection board CNT9-E
3MS95 216 Reagent level sensor block
3MS09 221 LCD TTL buffer for MS9-5 mother board
3MS09 233-25 Power supply 25V
3MS95 241 Amplifier board PAB9-E
3MS95 245 Acquisition board CK1-E
3MS09 266 MS9-5 mother board
3MS09 281C TFT color screen
3MS09 282C Converter board for color backlight
3MS09 287 Color backlight
3MS09 812 Start cycle micro-switch
3MS09 815 Optical sensor

SOLENOID PARTS
3MS95 409 Syringe motor 2N/11.6

3MS09 410 Up/Down step motor
3MS09 411 Rotation step motor with gear box
3MS95 421 Main pump for MS9-5
3MS09 422 Silent block for compact compressor
3MS95 431 Power supply fan (power cord lg = 20cm)
3MS95 433 Main fan (power cord lg = 52cm)
3MS09 450 Solenoid valve 2V
3MS09 451 Solenoid valve 3V
3MS95 453 2 ways EV for draining
3MS95 454 3 ways EV for diluent

PLASTIC PARTS
3MS95 120E MS9-5 plastic front cover (nude)

3MS95 310 RBC & Eo measuring chambers block for MS9-5
3MS95 311 WBC measuring chamber block for MS9-5
3MS09 330 Measuring lateral chamber
3MS95 331 Double measuring chambers lateral part
3MS09 340 Predilution cuvette
3MS09 352 Blood tube plastic starter
3MS95 360 MS9-5 plexi head of syringe pump
3MS95 361 Syringe pump black plastic basis
3MS95 392 MS9-5 black optical coding wheel

METALLIC PARTS
3MS95 110 Grey color metallic basis

3MS95 112 Grey color metallic master cover

RING PARTS
3MS09 320 Aperture holder set of O-rings

3MS09 610C ∅1.5 TEFLON ring for syringe pump
3MS95 613 Eo lysing reagent syringe pump ring
3MS09 614 Lysing ring
3MS95 618 Needle cleaning head ring
3MS95 619 Needle cleaning head TEFLON ring
3MS09 620 Diluent piston ring
3MS95 905 Syringe set of O-rings (610C + 613 + 614 + 620)

TUBE PARTS
3MS95 901 Reagents tubing kit for MS9-5

3MS95 907 MS9-5 set of tubes for maintenance

COMPUTER PARTS
3MS95 081 Hard disk microdrive 512Mo

3MS95 715 RAM 128Mo for mother board
3MS09 970 French
3MS09 971 US
3MS09 972 Cyrillic Alphanumeric keyboard
3MS09 973 Spanish
3MS09 974 Danish
3MS95 982 IDE CD-R/RW drive
3MS95 983 Mouse for MS9-5

ACCESSORIES
3MS09 560A Blood clot filter

3MS95 845 Logo stick for front cover
3MS09 890 Special grease for syringe
3MS95 190 Probe test cable for PAB9-E
3MS09 805 Main power switch + filter
3MS95 847 Anti glare filter for screen
3MS09 979 Keyboard protection
3MS95 KIT Maintenance kit within :
1 Acquisition board CK1-E
1 FK1-E fluidic board
1 Amplifier board PAB9-E
1 MS9-5 mother board
1 Power supply MS9(-5) 25V
2 Start cycle micro-switch
1 RBC & Eo measuring chambers block for MS9-5
1 WBC measuring chamber block for MS9-5
1 Photometer complete
1 2 ways EV for draining
1 3 ways EV for diluent
1 Solenoid valve 2V with expansion inserts
1 Solenoid valve 3V with expansion inserts
1 Reagent level sensor block

1 Blood clot micro-filters (12/box)
1 ∅60µm RBC aperture complete
1 ∅80µm WBC/Eo aperture complete
2 Syringe set of O-rings
2 Needle cleaning head ring
2 Needle cleaning head TEFLON ring
1 Set of tubes for maintenance
1 Special grease for syringe



BIOS SETUP

AWARD BIOS SETUP

1) STANDARD CMOS SETUP
2) BIOS FEATURES SETUP

3) CHIPSET FEATURES SETUP
4) POWER MANAGEMENT SETUP

5) PNP/PCI CONFIGURATION
6) INTEGRATED PERIPHERALS



ANNEXES

INDEX
A
Accessories 8-13, 182
Aperture 104-107, 112, 130, 140, 168, 174, 179, 182
B
Blood clot filter 12, 50, 182
Bubble 68, 70, 126, 134, 168
Buffer tube 48, 56, 124, 133
Buzzer 88
C
Calibration 2, 78, 84, 86, 106, 108, 110, 140, 148, 152
Cap 20, 30, 32, 36, 169
CD-R/RW 12, 18, 44, 88, 96, 181
CK1-E 24, 44, 76, 86, 106, 108, 170, 175, 182
Clot control 138
CNT9-E 76, 82, 96-99, 175
D
Detergent 54, 72, 104, 130-134
Diluent 42, 52, 70, 72, 104, 124, 130-134, 176, 179, 182
Dilution 52, 60, 64, 68-72, 104, 120, 123-126, 129-135, 140, 146, 152,
168
E
Electrical noise 78
F
Fan 44, 96, 176
FK1-E 24, 44, 48, 62, 76, 82, 84, 96, 130-135, 169, 175, 182
Fuse 22
G
Gain 78, 84, 108, 140, 152
Ground connection 50
H
Hemoref 32, 120, 130, 146, 168
Histogram 116-119, 138, 140, 146-149, 154-15, 160

I
IDE bus 88, 181
K
Key 12, 18, 30
Keyboard 10, 12, 16, 24, 28, 44, 88, 181-182
L
Lateral chamber 54, 106, 134, 177
LCD TTL buffer 88, 90, 175
LED control 84, 86
Level sensor block 40, 48, 52-61, 82, 175, 182
Lyse 32, 56, 130, 132-133, 168
Lyse Eo 32, 58, 130-131, 168
M
Manual 8, 10, 12
Measuring chamber 40, 48, 68, 78, 104, 106, 124, 126, 174, 177, 182
Measuring probe 78-81
Microdrive 88, 181
Mother board 44, 76, 88, 90, 96, 134, 175, 181, 182
Motor 82, 84, 129, 134, 169
peristaltic pump 42
rotation 42, 82, 126, 176
syringe 42, 82, 126, 129, 174, 176
up / down 42, 82, 126, 129, 176
Mouse 12, 24, 28, 181
N
Needle 66, 70, 124, 126, 129, 131-132, 168, 174, 179, 182
O
Offset 78, 108
Optical coding wheel 40, 177
P
PAB9-E 76-81, 86, 106, 175, 182
Parallel port 24, 88
Passive bus 44, 96, 175
Peristaltic pump 42, 48, 60, 68-73, 82, 84, 129, 135, 168
Photometer 40, 48, 60, 64, 72, 78-81, 108, 120, 126, 130, 134-135, 168,
174, 182
Power supply 2, 12, 22, 28, 44, 80, 94-99, 175, 182
PowerCell 86, 110-113, 116
Predilution 68, 72, 168
Predilution cuvette 168, 177

Predilution cuvette 40, 48, 70, 124, 129, 132

Pressure 54, 62, 66, 72, 129-130, 134-135, 168
sensor 48, 62, 72, 84
Priming 106, 126, 130, 132-133, 168
Printer 24, 28, 88
Probe voltage 78, 80, 106-111, 138-142, 152
Pulse 104, 108-117
Pump 44, 48, 62, 66, 72, 82, 84, 96
R
Reagent pack 34
Reagent tube 12, 32, 36, 52-71
S
Salt crystals 70
Sampling probe 48, 52, 66, 70, 72, 129-135, 174
Screen 16, 130, 174-175, 182
Screen cable 44, 92-93, 98
Sensor 50, 51, 68, 82, 84, 120, 126, 133, 134, 168, 175
Serial port 88
Smart card 12, 24, 34-36, 84
Smoothing 118-119, 150, 152
Syringe 40, 42, 48, 68, 82, 84, 126, 129-134, 168, 174-177, 179, 182
T
Threshold 114, 148, 170
Tube adapter 12, 174
Tube holder 16, 18, 40, 130-132, 135
U
USB port 24, 88
V
Vacuum 54, 60-69, 72, 104, 125-126, 129-131, 133-135, 140, 168
Valve 42, 48, 52-73, 82, 84, 129-135, 176, 182
diluent 42, 52, 72, 131-134, 176
waste 42, 72, 156, 176
W
Waste chamber 42, 54, 62, 64, 66, 129-130, 133-135, 174
Waste container 36
X
X/Y system 40, 169, 174
Y
Y adapter 12, 24, 28


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MELET SCHLOESING Laboratoires MS9-5® Reference Manual / 09-02 Page : 1

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

CELL MEASUREMENT 103

ERROR CODES 167

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

REF. SPARE PARTS 173

BIOS SETUP 185

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

Unlock the two latches. 

Open the lid to access to MS9-5 and

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

UNPACKING THE MS9-5

 To extract the MS9-5 from the transport box,

 Take the keyboard and the accessories

Store the transport box in a safe place for

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

Pull out by hands the protection plastic film which

Check accessories as follows :

 1 alphanumeric keyboard inside a plastic

 1 mouse with a Y adapter,

 2 plastic pockets with :

 A plastic pocket with :

 A plastic pocket with :

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

 A large polychrome back lighted

 A plastic front cover,

 A whole blood tube holder which is

 A compact alphanumeric keyboard.

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

 Insert the key inside the lock to open

 A whole blood tube holder which is

 A hole for the whole blood sampling

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

 6 plastic Luer lock fittings with cap

 1 exhaust tube for air transfer,

 A multi connection panel.

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

 Hinges for the upper front cover,

 Internal fixation holes,

 Electric main power supply plug ()

 Air flow filter.

Fuse type which must be used :

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

BP 508-OSNY - 95528 CERGY-PONTOISE / FRANCE Tel.: 33 1 30 75 30 00 Fax.: 33 1 30 73 17 80

 External DIN keyboard connector

 DB9 serial connector COM2,

 Parallel printer connector LPT1,

 External VGA video monitor

Unlock the two latches.

To extract the MS9-5 from the transport box,

Take the keyboard and the accessories

1 alphanumeric keyboard inside a plastic

1 mouse with a Y adapter,

2 plastic pockets with :

A plastic pocket with :

A plastic pocket with :

A large polychrome back lighted

A plastic front cover,

A whole blood tube holder which is

A compact alphanumeric keyboard.

Insert the key inside the lock to open

A whole blood tube holder which is

A hole for the whole blood sampling

6 plastic Luer lock fittings with cap

1 exhaust tube for air transfer,

A multi connection panel.

Hinges for the upper front cover,

Internal fixation holes,

Electric main power supply plug ()

Air flow filter.

External DIN keyboard connector

DB9 serial connector COM2,

Parallel printer connector LPT1,

External VGA video monitor

LAN RJ45 connector (10/100 Mb/s

The 'MS-Card' reader on FK1-E

The CK1-E board,

PS/2 connector for mouse AND

DB9 serial connector : COM1.

Connection of the alphanumeric keyboard

,, Connection of the mouse (with Y

Connection of the power supply cord.

Put the keys on the lock on the right side

Open the upper front cover by turning the

Unscrew the plastic cap of the reagent

Take the 4 transportation stickers off (yellow

Check that the plastic protection cap of

Install by screwing in a clockwise direction

Check the reagent tubes and color

Open the MS-Card carton pocket placed

The plastic MS-Card holder appears.

Take the plastic MS-Card holder out and

Insert the MS-Card in the card reader

As for the smart card MS-Card, open the

4 apertures will be seen +1 for Eo-Diff.

Unscrew the reagent bottles caps which

Insert the reagent tubes with their cap in

Install the waste container and place it

The dilution syringe pump,

The X/Y system with the sampling probe,

The whole blood tube holder,

The predilution cuvette,

The reagent level sensor block,

The WBC measuring chamber

The hemoglobin photometer,

The optical coding wheel.

The diluent solenoid valve,

The solenoid valves block,

The upper connection board,

The up/down stepper motor,

The waste chamber,

The syringe pump stepper motor,

The rotation stepper motor,

The peristaltic pump motor,

The amplifier board,

The waste solenoid valve,

The power supply cooling fan,

The power supply,