Scribd Logo
Upload

Welcome to Scribd!

  • 30K views

    Biology 2nd Lab Report (Onion Cell)

    Uploaded by

    Johanna Haludilu
    1. The document describes an experiment to prepare stained temporary mounts of onion peel cells and observe them under a microscope. Onion cells were stained with safranin dye and mounted on a slide with glycerine to view their structures. 2. Key structures observed in onion cells included the cell wall, nucleus located at the cell periphery, cytoplasm surrounding a large central vacuole, and distinct regularly shaped cells lined up side by side. 3. The experiment allowed the student to learn how to prepare slides of plant cells and observe the different cellular structures that distinguish plant cells, like cell walls and large central vacuoles.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Biology 2nd Lab Report (Onion Cell)

    Uploaded by

    Johanna Haludilu
    30K views4 pages
    1. The document describes an experiment to prepare stained temporary mounts of onion peel cells and observe them under a microscope. Onion cells were stained with safranin dye and mounted on a slide with glycerine to view their structures. 2. Key structures observed in onion cells included the cell wall, nucleus located at the cell periphery, cytoplasm surrounding a large central vacuole, and distinct regularly shaped cells lined up side by side. 3. The experiment allowed the student to learn how to prepare slides of plant cells and observe the different cellular structures that distinguish plant cells, like cell walls and large central vacuoles.

    Original Description:

    Onion cell lab report notes

    Original Title

    Biology 2nd lab report (onion cell)

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    1. The document describes an experiment to prepare stained temporary mounts of onion peel cells and observe them under a microscope. Onion cells were stained with safranin dye and mounted on a slide with glycerine to view their structures. 2. Key structures observed in onion cells included the cell wall, nucleus located at the cell periphery, cytoplasm surrounding a large central vacuole, and distinct regularly shaped cells lined up side by side. 3. The experiment allowed the student to learn how to prepare slides of plant cells and observe the different cellular structures that distinguish plant cells, like cell walls and large central vacuoles.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    30K views4 pages

    Biology 2nd Lab Report (Onion Cell)

    Uploaded by

    Johanna Haludilu
    1. The document describes an experiment to prepare stained temporary mounts of onion peel cells and observe them under a microscope. Onion cells were stained with safranin dye and mounted on a slide with glycerine to view their structures. 2. Key structures observed in onion cells included the cell wall, nucleus located at the cell periphery, cytoplasm surrounding a large central vacuole, and distinct regularly shaped cells lined up side by side. 3. The experiment allowed the student to learn how to prepare slides of plant cells and observe the different cellular structures that distinguish plant cells, like cell walls and large central vacuoles.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 4
    At a glance
    Powered by AI
    The key takeaways from the experiment are that plant cells have distinct cell walls, nuclei, cytoplasm and large vacuoles at their center. Temporary mounts of onion peel cells were prepared to observe these structures under a microscope.

    The main components of a plant cell observed in onion peel cells are the cell wall, cell membrane, cytoplasm, nucleus and a large vacuole present at the center surrounded by cytoplasm.

    The steps followed to prepare the temporary mount were peeling onion skin, staining it with safranin, placing it on a slide with glycerine, adding a cover slip and viewing under a microscope.

    TEXILA AMERICAN UNIVERSITY

    Name: Johanna N. Haludilu


    Date: 22 April 2020
    Module: Biology I
    Experiment: 2
    Title: Plant cells (Onion cells)
    Lecturer: Dr Zulu
    Onion cells

    Aim
    To prepare strained temporary mounts of onion peel cells and record the observations.

    Introduction
    An onion is a multicellular plant organism. As in all plant cells, the cell of an onion peel consists of a
    cell wall, cell membrane, cytoplasm, nucleus and a large vacuole. The nucleus is present at the
    periphery of the cytoplasm. The vacuole is prominent and present at the centre of the cell. It is
    surrounded by cytoplasm. The presence of a cell wall and a large vacuole are indicators that held
    identify plant cells, such as seen in the onion peel.

    Apparatus
    1. Glycerine, 2. Safranin solution, 3. Dropper, 4. Forceps, 5. Distilled water, 6. Needle & brush, 7.
    Cover slip, 8. Compound microscope, 9. Blotting paper, 10. Onion, 11. Glass slide, 12. Watch glass

    Precautions
    Use a brush to transfer the peel from one apparatus to another. Staining of peel should neither be
    too dark, nor too light. Extra glycerine stain should be removed using blotting paper.

    Procedures
    Pour some distilled water into a watch glass. Peel off a leaf from half a piece of onion and using the
    forceps, pull out a piece of transparent onion peel (epidermis) from the leaf. Put the epidermis in
    the watch glass containing distilled water. Take a few drops of safranin solution in a dropper and
    transfer this into another watch glass. Using a brush, transfer the peel into the watch glass
    containing the safranin solution. Let this remain in the Safranin solution for 30 seconds, so that the
    peel is stained. Take the peel from the Safranin solution using the brush and place it in the watch
    glass containing the distilled water. Take a few drops of glycerine in a dropper and pour 2-3 drops
    at the center of a dry glass slide. Using the brush, place the peel onto the slide containing glycerine.
    Take a cover slip and place it gently on the peel with the aid of a needle. Remove the extra glycerine
    using a piece of blotting paper. Place this glass side on the stage of the compound microscope and
    view it.

    Observations
    We observed that there are a large number of regularly shaped cells lying side by side and each cell
    has a distinct cell wall. A distinct nucleus is present on the periphery of each cell. Lightly stained
    cytoplasm is observed in each cell. A large vacuole is present at the center of each cell, and is
    surrounded by the cytoplasm.

    My plant cell observation (my own drawing)

    Conclusion
    In this experiment we learned that cell walls and large vacuoles are clearly observed in all the cells,
    the cells placed for observation are plant cells. We learned how to prepare slides and what
    solutions are used when preparing them. We also saw the different cells that make up plant cells
    and their shapes. Overall this experiment was very educational.
    References
    1. Manual of Secondary Science Kit for Classes IX and X - Published by NCERT
    2. http://www.indiastudychannel.com/projects/4421-To-prepare-stained-temporary-mount-
    onion-peel-record-observations.aspx
    3. //www.youtube.com/watch?v=zXAgbSzEADQ
    4. //www.youtube.com/watch?v=i2x3MKSJez4
    5. http://en.wikipedia.org/wiki/Cell_%28biology%29

    Questions
    1. Safranin
    2. Thin layer of a fleshy onion leaf
    3. Glycerine
    4. Prevents drying
    5. To highlight the cell organelles
    6. Inner side of cheek with a toothpick
    7. A (Methylene blue)
    8. Soaking with the filter paper
    9. iv, i, iii, ii
    10. Avoid entry of air bubbles
    11. Prokaryotes
    12. Vacuole
    13. Irregular
    14. Pinkish red
    15. Plasma membrane and nucleus

    You might also like