Dr.G.R.

Damodaran College of Science

(Autonomous, affiliated to the Bharathiar University, recognized by the UGC)Re-
accredited at the 'A' Grade Level by the NAAC and ISO 9001:2008 Certified
CRISL rated 'A' (TN) for MBA and MIB Programmes

I M.Sc [2019-2021]
Semester II
Core:BIOPROCESS TECHNOLOGY-258A
Multiple Choice Questions.

1. Bioprocess Engineering would include the work of

A. mechanical engineer
B. electrical engineer
C. industrial engineer
D. All.
ANSWER: D

2. Bioprocesses differs from chemical processes with respect to the following

A. Every product requires different substrate
B. It can be produced at ambient temperature and atmospheric pressure
C. No aseptic conditions are needed
D. None of the above
ANSWER: B

3. Which of the following is NOT a method for gene transfer in bacteria?

A. Translocation.
B. Conjugation.
C. Transformation.
D. Transduction.
ANSWER: A

4. Following are the characteristics of industrial strains, A. It should withstand rapid change of temperature
and pH B. It should be able to use a wide variety of substrates C. It should be genetically stable D. It should
have a longer fermentation time
A. A,C,D are correct
B. A,B C are correct
C. A,B,D are correct
D. A,B,C and D are correct
ANSWER: B

5. The cell wall of the prokaryotes is unique and is composed of ________.

A. Cellulose.
B. Peptidoglycans.
C. Fungus cellulose.
D. Peptide.
ANSWER: B

6. Which one of the following is a malt sugar?

A. Isomaltose.
B. Maltose.
 C. Lactose.
D. Starch.
ANSWER: B

7. Grams of ethanol that can be produced from 100 grams of glucose is

A. 92
B. 51.1
C. 180
D. 46
ANSWER: B

8. Pick out the non-protein aminoacid from the following.

A. L-ornithine.
B. Asp.
C. His.
D. Asn.
ANSWER: A

9. The primary bond of protein is _______ bond.

A. Ionic.
B. Hydrophobic.
C. Peptide.
D. Disulfide.
ANSWER: C

10. Following is the method to keep the organisms metabolically active, except
A. Overlaying culture with mineral oil
B. Periodic transfer to fresh media
C. Saline suspension
D. Lyophilization
ANSWER: D

11. Which of the following amino acid is the smallest of all amino acid?
A. Gly.
B. Val.
C. Ala.
D. Ser.
ANSWER: A

12. Role of precursors in the fermentation medium is to

A. Provide buffering capacity
B. Enhance the product yield
C. Avoid foaming
D. Avoid contamination
ANSWER: B

13. Which of the following amino acid is actually an imino acid?

A. Glu.
B. Ala.
C. Pro.
D. Trp.
ANSWER: C

14. Which of the following amino acid is the largest of all the amino acid?
 A. Glu.
B. Tyr.
C. Trp.
D. His.
ANSWER: A

15. The two repeating subunits of the peptidoglycan layer are __________.
A. N-acetyl glucosamine.
B. N-acetyl muramic acid.
C. N-acetyl glycosamine.
D. both a and b.
ANSWER: D

16. Following is not an example of nitrogen source

A. Molasses
B. Urea
C. Yeast extract
D. Soy meal
ANSWER: A

17. Following are the methods of industrial strain improvement

A. Genetic recombination
B. Mutation by chemical agents
C. Mutation by UV radiation
D. All of the above
ANSWER: D

18. Organisms that prefer temperatures above 50°C are called _______.
A. thermophiles.
B. acidophiles.
C. alkalinophiles.
D. all the above.
ANSWER: A

19. Which of the following belongs to continuous culture?

A. Turbidostat.
B. Chemostat.
C. both a and b.
D. All the above.
ANSWER: D

20. A culture medium which contains known specific kinds and amounts of chemicals is a _______ medium.
A. liquid.
B. solid.
C. selective
D. defined synthetic.
ANSWER: D

21. The following material gives the austenitic (smoothness) properties in stainless steel
A. 8% nickel
B. 12% chromium
C. 10% aluminium
D. None of the above
ANSWER: A
22. Function of draft tube in airlift reactor is to
A. Decrease the pressure
B. Decrease the velocity of fluid
C. Improve the mass transfer
D. Minimize the kinetic energy
ANSWER: C

23. A culture containing only one kind of microorganism is called _______ culture.
A. synchronous.
B. pure
C. batch
D. continuous.
ANSWER: B

24. In which of the following bioreactors, the particles are not suspended in liquid
A. Air-lift reactor
B. Stirred vessel
C. Fluidized bed reactor
D. Trickle-bed
ANSWER: D

25. _______is referred as biological indicator of autoclave.

A. Bacillus stearothermophilus.
B. Bacillus substilis.
C. Bacillus megaterium.
D. Bacillus cereus.
ANSWER: A

26. Gas transfer calculations are often based on_____________ .

A. one-film theory.
B. three- film theory.
C. two-film theory.
D. four-film theory.
ANSWER: C

27. Which organism needs a lower concentration of oxygen in their environment for growth?
A. Anaerobe.
B. Aerobe.
C. Microaerophilic.
D. Macroaerophilic.
ANSWER: C

28. Space-time of 3 h for continuous flow reactor means that

A. Three reactor volumes of feed can be processed every hour
B. The time required to process one reactor volume of feed is 3 h.
C. It takes 3 h to dump the entire volume of the reactor with feed
D. Substrate conversion is 100% after three hours
ANSWER: B

29. The growth curve obtained for a medium containing two carbon sources is called ____________growth
curve.
A. diauxic.
B. continuous.
 C. batch.
D. synchronous.
ANSWER: A

30. Biomass yield (Yx/s) can be expressed as

A. Yx/s=g of cells produced/g of product produced
B. Yx/s=g of cells produced/g of residual substrate
C. Yx/s=g of cells produced/g of substrate consumed
D. none of the above
ANSWER: C

31. The organism that catalyses acetone-butanol fermentation is ________.

A. Acetobacter aceti
B. Penicillium chrysogenum.
C. Citrobacter sp.
D. Clostridium acetobutylicum.
ANSWER: D

32. The mechanism by which filtration occurs is/are ______.

A. inertial impaction.
B. diffusion.
C. electrostatic attraction.
D. all the above.
ANSWER: D

33. Which phase of bacterial growth represent the time of adaptation?

A. Lag.
B. Log.
C. Stationary.
D. Death.
ANSWER: A

34. The primary metabolites are synthesized in ________ phase.

A. log.
B. tropho.
C. deceleration.
D. death.
ANSWER: B

35. In ___________ phase, the secondary metabolites are synthesized.

A. exponential.
B. acceleration.
C. idio.
D. death.
ANSWER: C

36. Pick out the correct statement(s) from the following statements on stoichiometry of bioprocesses
A. Stoichiometry is based on the law of conservation of mass
B. The range of the thermodynamics coefficient for aerobic process is between 0.5 and 0.6
C. Both a & b
D. None of the above
ANSWER: C

37. Which among the following detergent is used for cell disruption?
 A. Sodium lauryl sulfate.
B. SDS.
C. Triton X-100.
D. All the above.
ANSWER: D

38. Carbon balance is generally calculated by working out the number of moles produced of a given product
per ____ grams of substrate utilized.
A. 200.
B. 100.
C. 1000.
D. 10.
ANSWER: B

39. A continuous culture in which growth rate is limited by the rate of nutrient supply is called ______.
A. turbidostat.
B. chemostat.
C. autostat
D. none of the above.
ANSWER: B

40. The transfer of genetic information by direct cell to cell contact is _____.
A. conjugation.
B. transformation
C. transduction.
D. transfection.
ANSWER: A

41. Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its
uses?
A. Restriction endonuclease - production of DNA fragments for gene cloning.
B. DNA ligase - enzyme that cuts DNA, creating sticky ends.
C. DNA polymerase - copies DNA sequences in the polymerase chain reaction.
D. Reverse transcriptase - production of cDNA from mRNA.
ANSWER: B

42. Which of the following seals the sticky ends of restriction fragments to make recombinant DNA?
A. Reverse transcriptase.
B. Restriction enzymes.
C. Gel electrophoresis.
D. DNA ligases.
ANSWER: D

43. Which organism can tolerate one environmental condition but still live in another?
A. Aerobes.
B. Anaerobes.
C. Facultative.
D. Endospore producing microbes.
ANSWER: C

44. Bacterial cells with F factor integrated in chromosomal DNA are termed as _____ cells.
A. P.
B. R
C. F.
 D. Hfr.
ANSWER: D

45. The sticky ends generated by restriction enzymes allow _____.

A. selection for plasmids lacking antibiotic resistance.
B. easy identification of plasmids which carry an insert.
C. replication of transfer RNA within the bacterial cell.
D. pieces of DNA from different sources to hybridize and ligate each other.
ANSWER: D

46. Which among the following represent aerobic process?

A. Trickling filters.
B. Rotating disc contactors.
C. Rotating drums.
D. All the above.
ANSWER: D

47. Which of the following mutations would be easiest to revert?

A. An insertion of 10base pairs.
B. A deletion of more than 10 base pairs.
C. A base pair substitution.
D. Insertion of a transposon.
ANSWER: C

48. What is gene therapy?

A. Pre-clinical testing for inherited diseases in newborns.
B. Treatment of diseases caused by genetic defect using genes.
C. Genetic engineering using recombinant DNA technology.
D. Cancer treatment using in vitro cultured stem cells.
ANSWER: B

49. Genome represents _______.

A. total number of chromosome found in an individual.
B. total number of genes of a chromosome.
C. the set of haploid chromosome found in each nucleus of a given species.
D. total number of genes required for its expression.
ANSWER: C

50. Which of the following is NOT an archaebacteria?

A. Halophiles.
B. Methanogens.
C. Cyanobacteria
D. Thermophiles
ANSWER: C

51. Potential source of hyaluronic acid is ________.

A. Streptococcus sp.
B. Staphylococcus sp.
C. Micrococcus sp.
D. Bacillus sp.
ANSWER: A

52. Which among the following is widely used in bakers yeast production?
A. Saccharomyces cerevisiae.
 B. Saccharomyces uvarum.
C. Saccharomyces carlsbergensis.
D. Candida species.
ANSWER: A

53. The tools of genetic engineering allow us to manipulate ________ directly.

A. RNA
B. DNA.
C. cell membranes.
D. cell wall.
ANSWER: B

54. The cell wall component methanochondroitin and pseudomurein are present in _____.
A. archaens.
B. eubacteria.
C. filamentous fungi
D. yeast.
ANSWER: A

55. Adsorption of organic compounds to the stationary phase such as silica gel is due to _______ forces.
A. vander waals
B. dipole.
C. H bonding.
D. all the above
ANSWER: D

56. ________ obtains energy by oxidation of organic and inorganic compounds.

A. Phototrophs.
B. Chemotrophs.
C. Autotrophs.
D. Heterotrophs.
ANSWER: B

57. _____ use reduced organic molecules as carbon source.

A. Organotrophs
B. Heterotrophs.
C. Autotrophs.
D. Lithotrophs
ANSWER: B

58. RFLP analysis is a technique that _____.

A. uses hybridization to detect specific DNA restriction fragments in genomic DNA.
B. is used to determine whether a gene is transcribed in specific cells.
C. measures the transfer frequency of genes during conjugation.
D. is used to detect genetic variation at the protein level.
ANSWER: A

59. Concentration of nitrogen source normally supplied in growth media is _____g/L.

A. 0.5.
B. 2.
C. 6.
D. 10.
ANSWER: B
60. Tower fermentor is mainly used for----- production.
A. Wine.
B. Beer.
C. Enzyme.
D. Vitamin.
ANSWER: B

61. Concentration of trace elements is ________ mg/L.

A. 0.5.
B. 1.5.
C. 2.5.
D. 3.5.
ANSWER: A

62. Which of the following is the most widely needed trace element for cell growth?
A. Cu.
B. Fe.
C. Mo.
D. Ni.
ANSWER: B

63. Trace element needed under specific growth conditions is _______.

A. Co.
B. Be.
C. Ti.
D. Al.
ANSWER: A

64. Trace elements that are rarely required are _______.

A. Be.
B. Al.
C. Ti.
D. All the above.
ANSWER: D

65. Plasmid and genomic DNA differ in density can be identified by _____ methods
A. Enzymatic digestion.
B. Caseium chloride gradients.
C. PEG separation.
D. Chromatographic.
ANSWER: B

66. Which one of the following represents carbon source?

A. Corn syrup.
B. Corn steep liquor.
C. soya meal.
D. Groundnut meal.
ANSWER: A

67. Abbreviation of GMP stands for ________.

A. Good Manufacturing Practice.
B. Good Making Practice.
C. Good Manufacturing Procedure.
D. Good Making Procedure.
 ANSWER: A

68. Degree of reduction of oxygen is ______.

A. 2.
B. -2.
C. 6.
D. -6.
ANSWER: B

69. Degree of reduction of phosphorous is ______.

A. 2.
B. 3.
C. 4.
D. 5.
ANSWER: D

70. What is the degree of reduction of sulfur?

A. 2.
B. 4.
C. 6.
D. 8.
ANSWER: C

71. Justify the statement:

A. gas specific and temperature dependent are not related.
B. gas specific and temperature dependent are dependent.
C. gas specific and temperature dependent are related.
D. all the above.
ANSWER: C

72. Chromatography is used to ______.

A. separate two or more compounds based on their polarities.
B. separate two or more compounds based on their masses
C. separate two or more compounds based on how strongly they interact with other compounds.
D. all the above.
ANSWER: D

73. A microarray is a/an _________.

A. ray of a small wavelength.
B. type of ultraviolet ray.
C. RNA probe used to identify viruses.
D. arrangement of oligonucleotide probes, closely arranged on a small solid support surface.
ANSWER: D

74. Activation energy for Bacillus stearothermophilus is _______ kcal/mol.

A. 70.
B. 67.7.
C. 56.5.
D. 42.7.
ANSWER: B

75. In column chromatography with a polar stationary phase such as silica gel and non-mobile phase
________ compounds are eluted first.
A. polar.
 B. non polar.
C. intermediate polar.
D. ionic.
ANSWER: B

76. The rate of ______ transfer is then multiplied by the volume of the aerator to yield the oxygen transfer
rate, a key input in diffuser design.
A. heat mass
B. oxygen deficit
C. oxygen mass
D. none of the above
ANSWER: C

77. Which among the following is an organic nitrogen source?

A. Ammonia gas.
B. Ammonium sulfate.
C. Urea.
D. Ammonium nitrate.
ANSWER: C

78. Which one of the following is used as both carbon and nitrogen source?
A. Corn steep liquor
B. Peanut meal
C. Soya meal.
D. Urea.
ANSWER: A

79. The precursor of penicillin V is _____.

A. propionate.
B. phenoxyacetic acid.
C. anthranilic acid.
D. dihydroxynovobionic acid.
ANSWER: B

80. The precursor of griseofulvin is _____.

A. chloride.
B. propionate.
C. D-Thr.
D. L-Thr.
ANSWER: A

81. Nocardia orientalis is used for the production of ________.

A. cyclosporine A.
B. organomycin A.
C. nikkomycin.
D. dimethylvancomycin.
ANSWER: D

82. The microorganism used for the production of riboflavin is _____.

A. Lactobacillus bulgaricus
B. Bacillus subtilis.
C. Streptomyces sp.
D. Serratia marcescens.
ANSWER: A
83. The inhibitor used in the production of tetracycline is _____.
A. sodium bisulfite.
B. bromide.
C. phosphate
D. penicillin.
ANSWER: B

84. Which of the following phenomena is NOT highly involved in removal of particles by non-fixed pore
filters?
A. Inertial impaction.
B. Diffusion.
C. Interception.
D. Electrostatic attraction.
ANSWER: C

85. According to The American Iron and Steel Institute (AISI) steels containing _____ percentage of
chromium are classified as stainless steel.
A. 4.
B. >4.
C. 3.
D. <1.
ANSWER: B

86. Pick out the electrical transducer used in bioreactor.

A. Potentiometric.
B. Amperometric.
C. Conductimetric.
D. All the above.
ANSWER: D

87. The chromium content of AISI grade 316 steels is ______ percent.
A. 15.
B. 18.
C. 25.
D. 28.
ANSWER: B

88. Which among the following is NOT an impeller?

A. Disc turbine.
B. Vaned discs.
C. Marine propeller.
D. Orifice sparger.
ANSWER: D

89. How many baffles are normally incorporated into agitated vessels to prevent vortex and to improve
aeration efficiency?
A. 1.
B. 2.
C. 3.
D. 4.
ANSWER: D

90. Which among the following is a perforated type of sparger?

 A. Porous sparger.
B. Orifice sparger.
C. Nozzle sparger
D. Combined sparger-agitator.
ANSWER: B

91. The saturation concentration for oxygen is calculated using __________ .

A. Lambert's Law
B. Henry's Law
C. Beer's Law
D. Kirby's Law
ANSWER: B

92. Addition of nutrients, acid/alkali to fermenter is normally made through _____tubes.

A. Steel.
B. Wooden.
C. Silicone.
D. Rubber.
ANSWER: C

93. In _________valve, a horizontal disc is raised/lowered in its seating to control the rate of flow.
A. Gate.
B. Piston.
C. Globe.
D. Needle.
ANSWER: C

94. In which type of valve a sliding disc is moved in/out of the flow path by turning the stem of the valve?
A. Gate.
B. Piston.
C. Globe.
D. Plug.
ANSWER: A

95. The valve commonly used to regulate the flow of water/steam is ________.
A. plug.
B. ball.
C. pinch.
D. globe.
ANSWER: D

96. The valve commonly used for ON/OFF application is ______.

A. butterfly.
B. ball.
C. pinch.
D. gate.
ANSWER: A

97. The valve commonly used to control crude flow is _____.

A. fire.
B. stick
C. ball.
D. gate.
ANSWER: D
98. The valve commonly used for accurate flow control is _______.
A. pinch.
B. globe.
C. needle.
D. diaphragm.
ANSWER: C

99. The valve commonly used for sterile uses is ________.

A. diaphragm.
B. globe.
C. needle.
D. butterfly.
ANSWER: A

100. The valve used to prevent accidental reversal of flow of liquid/gas in a pipe is _______ valve.
A. pressure control.
B. check.
C. safety.
D. gate.
ANSWER: B

101. Which of the following methods are used for polymer bead preparation?
A. Gelation.
B. Precipitation.
C. Polycondensation.
D. All the above.
ANSWER: D

102. Oxygen solubility________ as the temperature rises.

A. Inceases.
B. Not affected.
C. Decreases.
D. None.
ANSWER: C

103. Diauxic growth is the ability of microorganism to display ________ growth pattern.
A. 1.
B. 2.
C. 3.
D. 4.
ANSWER: B

104. The control system in which a sensor will detect change in output of the controller is proportional to the
input signal produced by environmental change is ____ controller.
A. two-position.
B. proportional.
C. integral.
D. derivative.
ANSWER: B

105. In ______ control the output signal is determined by the integral of the error input over the time of
operation.
A. ON/OFF.
 B. proportional
C. integral.
D. derivative
ANSWER: C

106. The controller which serves the rate of change of the error signal and contributes a component of the
output signal that is proportional to a derivative of the error signal is _____.
A. proportional
B. ON/OFF.
C. integral.
D. derivative.
ANSWER: D

107. The oxygen deficit (D) is the driving force for the establishment of __________ equilibria.
A. water-air
B. water-water
C. air-air
D. air-water
ANSWER: D

108. A ________ converts a digital signal from the computer into an electrical voltage.
A. DAC.
B. DDC.
C. ADC.
D. SSC.
ANSWER: A

109. Supervisory Set Point Control (SSC) is also called ________.

A. digital set point control.
B. digital to analogue converter.
C. analogue to digital converter.
D. direct digital control.
ANSWER: A

110. Which among the following has a constant viscosity regardless of shear?
A. Dilatant liquid
B. Bingham plastic.
C. Pseudoplastic.
D. Newtonian liquid.
ANSWER: D

111. In which of the following liquid the apparent viscosity decreases with increasing shear rate?
A. Bingham plastic.
B. Pseudoplastic.
C. Newtonian liquid.
D. Dilatant liquid.
ANSWER: B

112. Which of the following treatments removes phosphates and nitrates from sewage?
A. Primary.
B. Secondary.
C. Tertiary.
D. Digester.
ANSWER: C
113. The species used for citric acid production is _______.
A. Penicillium sp.
B. Aspergillus niger.
C. Candida albicans.
D. All the above.
ANSWER: D

114. Which among the following filter belong to continuous filter?

A. Rotary vacuum.
B. Vertical metal leaf.
C. Plate and frame.
D. Stacked-disc.
ANSWER: A

115. The riser tube and the down comer tubes are the features of ________ fermenter.
A. airlift.
B. packed bed.
C. tower.
D. bubble column.
ANSWER: A

116. The size of the E.coli genome is _____.

A. 4640 bp.
B. 4.64 Kbp
C. 4.64 Mbp.
D. not known with certainty.
ANSWER: C

117. Which among the following represent anaerobic process?

A. Towers.
B. Rotating biological contactors.
C. UASB.
D. Activated sludge process.
ANSWER: C

118. Rotating biological contactors are also called _______.

A. Rotating drums.
B. Rotating disc contactors.
C. UASB.
D. Fluidized bed.
ANSWER: B

119. _____________ is generally modelled by kinetic equations giving the time evolutions for biomass,
substrate, and product concentrations.
A. Fermentation.
B. Distillation.
C. Evaporation.
D. None of the above.
ANSWER: A

120. The stationary phase material in the column chromatography is also called _____.
A. adsorbent.
B. adsorb.
 C. elute.
D. absorbent.
ANSWER: A

121. The mechanism by heat transfer occurs is ________.

A. conduction.
B. convection.
C. radiation.
D. all the above.
ANSWER: D

122. In chromatography, sample components undergo _______ distribution between two phases.
A. equilibrium.
B. molecular.
C. adsorption
D. absorption.
ANSWER: A

123. In column chromatography the compound which adsorbed _______ eluted first.
A. strongly.
B. weakly.
C. with medium strength.
D. very strongly.
ANSWER: B

124. To separate the mixture of anions, _____ is used.

A. a cation exchange resin.
B. an anion exchange resin.
C. an amino acid analyzer.
D. a carbohydrate analyzer.
ANSWER: B

125. Paper chromatography is an example of ________ chromatography.

A. solid-solid
B. liquid-liquid.
C. gas-liquid.
D. solid-liquid
ANSWER: B

126. Reversed phase of HPLC is a _______ chromatography.

A. liquid-liquid.
B. solid-liquid.
C. gas-liquid.
D. solid-solid.
ANSWER: A

127. In reversed phase HPLC, the ________ polar compounds in the mixture are eluted first.
A. highly.
B. least.
C. intermediately.
D. none of the above.
ANSWER: A

128. The hydrogel obtained from natural polymers used for cell immobilization is _______.
 A. polyacrylamide.
B. methacrylate.
C. chitin.
D. polyurethane.
ANSWER: C

129. _____ is secreted by Pseudomonas elodea.

A. Pectin.
B. Gellan gum.
C. Chitin.
D. Carrageenans.
ANSWER: B

130. Red sea weeds serve as a source of _____.

A. alginate.
B. carrageenans.
C. chitin.
D. agarose.
ANSWER: B

131. Molecular weight cut off of a membrane is the molecular weight at which the membrane rejects _______
percentage of solute.
A. 50.
B. 70.
C. 90.
D. 100.
ANSWER: C

132. Which of the following transducer is used as Thermistor?

A. Electrical.
B. Optical.
C. Thermal.
D. Mechanical.
ANSWER: C

133. The aspect ratio of vessels used for microbial work will be ____.
A. 3:1.
B. 2:5.
C. 4:2.
D. 1:1.
ANSWER: A

134. Tachometer is used to control ______.

A. speed.
B. pH.
C. gas supply.
D. temperature.
ANSWER: A

135. The species used for acetone-butanol production is _______.

A. Clostridium sp.
B. Candida sp.
C. Saccharomyces cerevisiae.
D. Rhizopus sp.
 ANSWER: A

136. Rotameter is used to control ______.

A. velocity.
B. temperature.
C. gas supply.
D. all the above.
ANSWER: C

137. Optimal temperature for ethanol production using yeast is ____ degree celcius.
A. 30.
B. 40.
C. 50
D. 60.
ANSWER: A

138. Which among the following factors will affect oxygen transfer in fermenter?
A. Bubbles.
B. Sparging.
C. Antifoam agents.
D. all the above.
ANSWER: D

139. The major organism used hexoses as substrates for ethanol production is _____.
A. Saccharomyces cerevisiae.
B. Candida sp.
C. Clostridium sp.
D. Zymomonas sp.
ANSWER: A

140. ______organism used lactoses and pentoses as substrates for ethanol production.
A. Yeast.
B. Candida sp.
C. Penicillium sp.
D. Mucor sp.
ANSWER: B

141. The thermophile used in ethanol production is _____.

A. Kluyveromyces fragilis
B. Candida sp.
C. Thermus aquaticus.
D. Thermoanaerobacter ethanolicus.
ANSWER: D

142. The species widely used in industrial production of lactic acid is _______.
A. Lactobacillus sp.
B. Pediococcus sp.
C. Rhizopus sp.
D. Leuconostoc sp.
ANSWER: A

143. Optimal temperature for Lactobacillus delbruckii in lactic acid production is ____degree celcius.
A. 40.
B. 43.
 C. 46.
D. 50.
ANSWER: B

144. Which organism is called Brewers yeast?

A. Saccharomyces cerevisiae.
B. Saccharomyces uvarum.
C. Saccharomyces carlsbergensis.
D. Candida sp.
ANSWER: C

145. Which among the following is widely used in bakers yeast production?
A. Saccharomyces cerevisiae.
B. Saccharomyces uvarum.
C. Saccharomyces carlsbergensis.
D. Candida yeast.
ANSWER: A

146. The most common carbon and energy source for bakers yeast production is ___.
A. starch.
B. molasses.
C. soybean meal.
D. sulfite waste liquor.
ANSWER: B

147. In column chromatography with a polar stationary phase such as silica gel and non mobile phase
________ compounds are eluted first.
A. polar.
B. non polar.
C. intermediate polar.
D. ionic.
ANSWER: B

148. The formation of ________ is a crucial step in an anaerobic digestion.

A. hydrogen.
B. carbon dioxide.
C. water.
D. acetate.
ANSWER: D

149. Trickling filter is also known as ________ bed.

A. bacterial.
B. sewage.
C. retention.
D. salt.
ANSWER: A

150. Effluent from sewage treatment plants should have a _____ biological oxygen demand (BOD) value.
A. normal.
B. low.
C. no.
D. both a and b.
ANSWER: B
 Staff Name
Kalaigandhi V .