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    Disinfectants For The Classroom: Recommendations

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    Ayyu Thrye Sartheeqaa
    There are many types of disinfectants available, each with their own advantages and disadvantages. The document recommends using a 5-10% bleach solution to disinfect surfaces and bacterial cultures overnight, and a 1:100 or 1:200 dilution of bleach or Lysol to disinfect surfaces. While bleach is effective, other options like Lysol or chlorhexidine are better for student use since bleach can ruin clothing. Proper hand washing with soap is also important for students.

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    Disinfectants For The Classroom: Recommendations

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    Ayyu Thrye Sartheeqaa
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    There are many types of disinfectants available, each with their own advantages and disadvantages. The document recommends using a 5-10% bleach solution to disinfect surfaces and bacterial cultures overnight, and a 1:100 or 1:200 dilution of bleach or Lysol to disinfect surfaces. While bleach is effective, other options like Lysol or chlorhexidine are better for student use since bleach can ruin clothing. Proper hand washing with soap is also important for students.

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    There are many types of disinfectants available, each with their own advantages and disadvantages. The document recommends using a 5-10% bleach solution to disinfect surfaces and bacterial cultures overnight, and a 1:100 or 1:200 dilution of bleach or Lysol to disinfect surfaces. While bleach is effective, other options like Lysol or chlorhexidine are better for student use since bleach can ruin clothing. Proper hand washing with soap is also important for students.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    29 views1 page

    Disinfectants For The Classroom: Recommendations

    Uploaded by

    Ayyu Thrye Sartheeqaa
    There are many types of disinfectants available, each with their own advantages and disadvantages. The document recommends using a 5-10% bleach solution to disinfect surfaces and bacterial cultures overnight, and a 1:100 or 1:200 dilution of bleach or Lysol to disinfect surfaces. While bleach is effective, other options like Lysol or chlorhexidine are better for student use since bleach can ruin clothing. Proper hand washing with soap is also important for students.

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    Disinfectants for the Classroom

    There are many disinfectants available. The difficulty is that there is no perfect disinfectant: effective, safe,
    readily available and inexpensive. The table below highlights the types available and their advantages and
    disadvantages. Since there are no pathogens used in the classroom, most of the disinfectants described in the
    table should be fine; however, pine oil products should be avoided since they are ineffective in killing bacteria.

    Recommendations
    • To disinfect bacterial cultures, plates, swabs, pipettes, toothpicks and other contaminated materials soak
    them in 5-10% household bleach – leave overnight and then discard.
    • To disinfect surfaces, wipe thoroughly with 1:100 dilution of household bleach or 1:200 dilution of Lysol.
    • While bleach can be used by students to clean lab benches, it is better for students to use a disinfectant that
    will not ruin their clothing such as a phenol (Lysol) or chlorhexidine (Phisohex).
    • Keep disinfectant hand cleaner by the sink for use by students to clean their hands.

    TYPE BRAND ACTION SPECTRUM ADVANTAGES DISADVANTAGES


    NAMES
    Chlorine Clorox, Purex Effective against bacteria and viruses. Good disinfectant and Caustic, rapidly inactivated by organic
    (5.25% sodium Generally used at a 1:30 dilution of deodorizer. Inexpensive and material, loses effectiveness quickly,
    hypochlorite) ordinary household bleach (one ounce available. produces carcinogenic by-products, ruins
    per quart of water). Needs 5 min clothing.
    contact time. Rapidly inactivated by
    organic materials.

    Stabilized Oxyfresh Dent- Effective against bacteria and viruses. Good disinfectant and Not readily available. Expensive.
    chlorine dioxide Rapidly inactivated by organic deodorizer. Diluted solution Rapidly inactivated by organic material.
    materials. is safe. Concentrated chlorine dioxide fumes may be
    toxic to living tissue.
    Phenols Lysol (phenol*, Effective against many bacteria, fungi Good bacterial and viral High concentrations can irritate skin eyes and
    not benzyl and some viruses. Requires 10 min. disinfectant. Moderate cost. lungs. Does not kill all bacteria and viruses.
    ammonium Cl), contact time. May not work well in the May not work well in the presence of organic
    Stericol, O-Syl presence of organic material. material
    Staphene, 1-
    Stroke Environ,
    Chlorhexidine Nolvasan, Effective against most bacteria and Effective against bacteria; Not effective against Pseudomonas. Does not
    gluconates Phisohex, some yeast. Requires 10 min. contact non-toxic. Moderate-high kill spores or viruses.
    Virosan, time. Does not kill bacterial spores or cost.
    Hibitane, viruses.
    Quaternary Roccal-D, Cleaner and disinfectant. Effective against most Expensive to purchase initially, although it is
    ammonium Quintacide, Effective against many types of bacteria. Detergent helps to used in dilute solution (0.1% final). Does not
    compounds Parvosol, Hitor, bacteria, some viruses, but not against remove organic material. kill spores, fungi. Pseudomonas, or some
    Merquat, spores or fungi. Requires contact time Low toxicity. viruses.
    Cetylcide of 10 min.
    Glutaraldehydes Wavecide, Effective against bacteria, including Very stable and effective. Expensive. Difficult to obtain.
    Cidex, spores, and viruses. Works well in Can irritate skin, eyes and lungs.
    Sporcide, presence of organic material and is very
    Banacide, stable compared to other disinfectants.
    Sterol
    Alcohols Ethanol is effective against a wide Broad range, low cost, Requires long contact time and evaporates
    variety of pathogens if soaked for readily available, non-toxic quickly so items must be soaked in alcohol.
    sufficient time (20-30 min). Flammable.
    70% ethanol is considered most
    effective disinfectant.
    Iodines Vanodine, Effective against most bacteria, Good disinfectant. Readily Rapidly inactivated by organic materials.
    Betadyne, including spores, and some viruses. available. Long shelf life. Must be used in concentrated form, which
    Povidone, Requires 30 min. contact time. Rapidly Moderate cost. stains surfaces.
    Scrubodyne inactivated by organic materials.

    Pine oil Pine-Sol, Hexol Pine oils have some disinfectant Good for cleaning, low cost, Not effective in killing pathogens.
    properties, but they are not very non-toxic, readily available.
    effective.
    * Note that many Lysol products in the grocery store do not contain phenol. The effective form is the hospital strength Lysol that contains
    phenylphenol.

    Contributed by:
    Teresa Thiel
    Department of Biology
    University of Missouri-St. Louis

     1999 Science in the Real World: Microbes in Action


    This material may be duplicated by teachers for use in the classroom. Any other use is prohibited.

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