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    Crude Fiber Determination in Oatmeal: Application Note

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    Cyrille Otchoumou

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    Crude Fiber Determination in Oatmeal: Application Note

    Uploaded by

    Cyrille Otchoumou
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    90 views3 pages

    Crude Fiber Determination in Oatmeal: Application Note

    Uploaded by

    Cyrille Otchoumou

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    © All Rights Reserved
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    APPLICATION NOTE

    F&F-F-001-2017/A1

    Crude Fiber Determination in


    Oatmeal according to Weende
    Reference: AACC, Method 32-10.01 Crude Fiber in Flours, Feeds, and Feedstuffs

    Tested with VELP Scientifica FIWE 6 Fiber Analyzer (Code F30520200).

    Copyright © 2017 VELP Scientifica. All rights reserved.


    No part of this publication may be reproduced or transmitted in any form or for any purpose without the express permission of VELP.
    VELP Scientifica, Italy Tel: +39 039 628 811 Fax: +39 039 628 8120 www.velp.com
    CRUDE FIBER DETERMINATION IN OATMEAL

    Introduction
    Oats are a whole-grain cereal, known scientifically as Avena sativa. They are mainly grown in North America and
    Europe.
    This whole-grain are a very good source of fiber, and are high in vitamins, minerals and antioxidants.
    Oats contain more soluble fiber than other grains, leading to slower digestion, increased satiety an d suppression of
    appetite. Most of the soluble fiber is composed of beta-glucans.
    Beta-glucans are known to lower cholesterol levels and increase excretion of bile acids. They are also believed to cause
    a reduction in blood sugar and insulin levels after a carbohydrate-rich meal.
    Daily consumption of beta-glucans has been shown to lower cholesterol, especially LDL (the “bad”) cholesterol, and may
    therefore decrease the risk of heart disease.
    Oats also contain insoluble fibers, including lignin, cellulose and hemicellulose.

    Fiber Determination in Oatmeal according to Weende method


    The method is based on the solubilization (digestion) of non-cellulosic compounds by sulfuric acid and potassium
    hydroxide solutions. Crude fiber is the loss on ignition of the dried residue remaining after digestion of the sample and
    determined by weight difference. This method is applicable to grains, meals, flours, feeds, and fiber-bearing material
    from which fat can be extracted to leave workable residue.

    Reagents
    1- Sulfuric acid (H 2SO4) 1.25% - 0.255 ± 0.005 N. 12.5g, 98% concentrated to 1000 ml with distilled water.
    2- Potassium hydroxide (KOH) 1.25% - 0.223 ± 0.005 N, free from carbonate. 12.5 g to 1000 ml with distilled water.
    3- n-octanol as antifoam.
    3- Anhydrous acetone

    Sample
    Oatmeal Crude Fiber labeled value: 7.0 ± 0.6

    Analysis Procedure
    The diagram below shows the steps involved in the procedure:

    1. Dried the sample by heating in an oven at 105 °C to constant weight. Cool in a desiccator.
    2. Weight accurately 1 g about of dried and homogeneus sample (1 mm about) approximately with 1 mg (Msample ).
    3. Add 1.25% sulfuric acid up to the 150 ml notch, after preheating by RC2 hot plate (cod. F20700430) in order to reduce
    the time required for boiling.
    4. Add 3-5 drops of n-octanol as antifoam agent.
    5. Boil 30 minutes exactly from the onset of boiling.
    6. Connect to vacuum for draining sulfuric acid.
    7. Wash three times with 30 ml of hot deionized water (crucible filled up to the top), connecting each time to compressed
    air for stirring the content of crucible.
    8. After draining the last wash, add 150 ml of preheated potassium hydroxide (KOH) 1.25% and 3 -5 drops of antifoam.
    9. Boil 30 minutes.

    Copyright © 2017 VELP Scientifica. All rights reserved.


    No part of this publication may be reproduced or transmitted in any form or for any purpose without the express permission of VELP.
    VELP Scientifica, Italy Tel: +39 039 628 811 Fax: +39 039 628 8120 www.velp.com
    CRUDE FIBER DETERMINATION IN OATMEAL

    10. Filter and wash as point 7.


    11. Perform a last washing with cold deionized water aimed to cool the crucibles and then wash three times the crucible
    content with 25 ml of acetone, stirring each time by compressed air.
    12. Remove the crucibles and determine the dry weight after drying in an oven at 130 °C for 2 hours or at 105 °C for at
    least 8 hours. Let cool in a desiccator. This weight represents the crude fiber plus ash content ( Mdry).
    13. The crucibles are then placed in a muffle and heat up to 525 °C for three hours and reweighed after cooling in a
    desiccators (Mash ).
    14. Remove ash and if necessary clean the crucibles by an oxidizing procedure.

    Calculation

    % Crude Fiber = Mdry - Mash * 100


    Msample
    Msample = sample weight
    Mdry = crucible weight with fiber and ashes, after drying in an oven at 130 °C for 2 hours
    Mash = crucible weight with ashes, after muffle at 525 °C for three hours

    Typical Results on Oatmeal

    Msample (g) Mdry (g) Mash (g) Fiber %

    0.985 30.5253 30.4533 7.31


    1.010 30.4192 30.3444 7.41
    0.968 30.3726 30.3001 7.49
    0.970 30.3752 30.3043 7.31
    1.024 29.9166 29.8414 7.35
    0.970 30.4862 30.4146 7.38
    Average ± SD% 7.37 ± 0.07
    RSD% * 0.9
    Fiber Labeled Value: 7.0 ± 0.6%
    * RSD% = (Standard Deviation * 100) / Average

    Conclusion
    The obtained results are reliable and in accordance with the labeled value. The use of an extraction apparatus purposely
    devised for this method as FIWE unit, makes very easy the standardization of analytical conditions. The FIWE Series is
    suitable for Crude Fiber (CF), Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF) and Acid Detergent Lignin
    (ADL).

    Benefits of FIWE are:


     3 or 6 positions simultaneously: FIWE units can support up to 3 (FIWE 3) or 6 (FIWE 6) crucibles.
    Samples can also be processed individually
     Time saving: fast analysis (2 hours with FIWE vs. 6 hours manually)
     Easy to use: convenient filtration, with pump and air pressure
     Precision and accuracy: high reproducibility of the results: ± 1% relative or better

    In order to avoid losses of fiber, it’s important to remember that crucibles life is around 20 -30 analysis, because the
    fritted filter could be damaged from basic and acid solutions. Hence it’s suggested to change them after 20-30 analysis.

    Copyright © 2017 VELP Scientifica. All rights reserved.


    No part of this publication may be reproduced or transmitted in any form or for any purpose without the express permission of VELP.
    VELP Scientifica, Italy Tel: +39 039 628 811 Fax: +39 039 628 8120 www.velp.com

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