IB Biology Lab "Tool-Kit"

IB Biology Lab “Tool-Kit” NAME: __________________________________
Lab Safety
According to Issaquah School District policy, each student must have returned a lab safety contract, signed by student
and parent before being allowed to do lab work.
PERSONAL SAFETY
 Every person shall wear eye protection devices when using corrosive, toxic, reactive, or irritating chemicals and during
hazardous activities.
 Wash your hands before removing your goggles and leaving the laboratory.
 Never be a practical joker in the laboratory.
 Laboratory aprons must be worn when working with hazardous solutions.
 If you do not understand how or why to do a task, ask your instructor for help. If there is any doubt in your mind, ask
your instructor.
 Some chemicals can be harmful to a fetus. If pregnant, or suspect pregnancy, notify your science teacher or school
counselor.
 Tie back long hair. Secure loose clothing to the body and remove loose fitting coats and jackets.
 In classrooms functioning as a laboratory, no eating or drink will be allowed.
ROOM SAFETY
 Learn the location of the eyewash station and fire extinguishing devices and how to use them.
 Before leaving the laboratory, be certain that gas and water lines at your station are shut tightly and that all equipment
and supplies are placed in their proper places.
 Off-limits designation for any area or demonstration is to be strictly observed by students.
 Know the emergency evacuation route and meeting point.
LAB SAFETY SKILLS

 Follow your instructor’s oral and written instructions before, during and after all laboratory
activities.
 Read an assigned investigation carefully before beginning it, noting all cautions listed.
 Work deliberately and with definite purpose, but do not hurry.
 Keep the workspace at your station and your apparatus clean and in good order.
 Read the label carefully before taking anything from a bottle or container. Using the wrong material could result in
serious injury.
 Know what you are doing. Be wary of what neighboring students are doing.
MATERIALS SAFETY
 All chemicals should be regarded as hazardous unless your instructor informs you otherwise.
 Never mix or heat chemicals unless you are directed to do so.
 When mixing concentrated acids and water, always pour acids into water slowly and stir constantly.
 When observing the odor of any liquid, do not smell it directly. Use your hand to fan the odor towards you.
 Never taste a chemical or a solution or touch chemicals with your hands unless directed to do so by your instructor.
 When heating a test tube, do not heat just one spot on the test tube. Never have the open end of the test tube pointed
at anyone. Never look directly down into a test tube.
 Always allow ample time for cooling after materials have been heated.
 Dispose of all materials as instructor designates.
NOTIFY YOUR INSTRUCTOR OF ANY ACCIDENT OR POTENTIALLY HAZARDOUS SITUATION.
IF ANY CHEMICAL IS SPLASHED ON YOU SKIN OR IN YOUR EYES,

FLOOD WITH WATER IMMEDIATELY, THEN NOTIFY YOUR INSTRUCTOR
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EXPERIMENTAL DESIGN
Step 1: Observation
 Many types of observation can be made on biological systems. They may involve:
o observation of certain behaviors in wild populations
o physiological measurements made during previous experiments
o ‘accidental’ results obtained when seeking answers to completely unrelated questions
 The observations may lead to the formation of questions about the system being studied
Step 2: Defining the Problem

Every scientific investigation begins with the question that the scientist wants to answer. The questions addressed by scientific
inquiry are based on observations or information gained through previous research. Just because a question can be answered
doesn’t mean that it can be answered scientifically.
Which of the following do you think can be answered by scientific inquiry.

 What is the cause of AIDS?
 Are serial killers evil by nature?
 Why is the grass green?
 What is the best recipe for chocolate chip cookies?
 When will the Big Earthquake hit San Francisco?
 How can the maximum yield be obtained from a peanut field?
 Does watching television cause children to have shorter attention spans?
How did you decide what questions can be answered scientifically?
Step 3: Identifying the Responding (Dependent) Variable

The responding variable is what the investigator measures (or counts or records). It is what the investigator thinks will vary during
the experiment. For example, she may want to study peanut plant growth. One possible responding variable is the height of the
peanut plants.
Name some other aspects of peanut plants growth that can be measured:



All of these aspects of peanut plant growth can be measured and can be used as responding variables in an experiment. There are
different responding variables possible in an experiment. The investigator can choose the one she thinks is most importance, or she
can choose to measure more than one responding variable.
Step 4: Identifying the Manipulated (Independent) Variable

The manipulated variable is what the investigator deliberately varies during the experiment. It is chosen because the investigator
thinks it might affect the responding variable.
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Name some factors that might affect the numbers of peanuts produced by peanut plants:



In many cases, the investigator does not change the manipulated variable directly. She collects data and uses the data to evaluate
the hypothesis, rather than doing a direct experiment. For example, the hypothesis that more crimes are committed during a full
moon can be tested scientifically. The number of crimes committed is the responding variable and can be measured from police
reports. The phase of the moon is the manipulated variable. The investigator cannot deliberately change the phase of the moon, but
can collect data during any phase he chooses.
Although many hypotheses about biological phenomena cannot be tested by direct manipulation of a variable, they can be
evaluated scientifically by collecting data that could prove the hypothesis false. This is an important method in the study of
evolution, where the investigator is attempting to test hypotheses about events of the past.
The investigator can measure as many responding variables as she thinks are important indicators of peanut growth. By contrast she
must choose only one manipulated variable to investigate in an experiment. For example, if the scientist wants to investigate the
effect that the amount of fertilizer has on peanut growth, she will use different amounts of fertilizer on different plants; the
manipulated variable is amount of fertilizer.
Why is the scientist limited to one manipulated variable per experiment?
Time is frequently used as the manipulated variable. The investigator hypothesizes that the responding variable will change over the
course of time. For example, he may want to study the diversity of soil bacteria found during different months of the year. However,
the units of time used may be anywhere from seconds to years, depending upon the system being studied.
Identify the manipulated and responding variables in the following examples.
 Height of bean plants is recorded daily for 2 weeks.

Manipulated variable:______________________________________
Responding variable:_______________________________________
 Guinea pigs are kept at different temperatures for 6 weeks. Percent weight gain is recorded.
Manipulated variable:________________________________________
 The diversity of algal species is calculated for a coastal area before and after an oil spill.
 Light absorption by a pigment is measured for red, blue, green, and yellow light.
Step 5: Identifying the Controlled Variables

A third type of variable is the controlled variable. Controlled variables are factors that are kept equal in all treatments, so that any
changes in the responding variable can be attributed to the changes the investigator made in the manipulated variable.
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Materials used and measurement techniques are NOT controlled variables (they are validity measures). While materials and
techniques must be consistent, a true variable is something that could directly influence the responding variable, not just how it is
measured.
Since the investigator's purpose is to study the effect of one particular manipulated variable, he must try to eliminate the possibility
that other variables are influencing the outcome. This is accomplished by keeping the other variables at constant levels, in other
words, by standardizing these variables. For example, if the scientist has chosen the amount of fertilizer as the manipulated variable,
he wants to be sure that there are no differences in the type of fertilizer used. He would use the same formulation and same brand
of fertilizer throughout the experiment.
What other variables would have to be standardized in this experiment?
List three validity measures that could be used in this experiment.
Step 6: Writing the Hypothesis

A hypothesis is simply a statement of the scientist’s educated guess at the answer to the question. The nature of science is such that
we can prove a hypothesis false by presenting evidence from an investigation that does not support the hypothesis. But we cannot
prove a hypothesis true. We can only support the hypothesis with evidence from this particular investigation.
Scientific knowledge is thus an accumulation of evidence in support of hypotheses: it is not to be regarded as absolute truth.
Hypotheses are accepted only on a trial basis. Future investigations may be able to prove any hypothesis false. Current scientific
studies you read about in the newspaper (for example, investigations of the effects of caffeine) are sometimes quite preliminary and
therefore tentative in nature. Often, studies are published whose results contradict each other. However, this does not mean that
scientific knowledge is flimsy and unreliable. Much of the information in your textbook, for example, is based upon many
experiments carried out by numerous scientists over a period of time.
The scientific method, then applies only to hypotheses that can be proven false through experimentation. (There are other types of
scientific investigation, such as observation and comparison that do not involve hypothesis testing.) It is essential to understand this
in order to understand what is and is not possible to learn through science. Consider, for example, this hypothesis: More people
behave immorally when there is a full moon than at any other time of the month. The phase of the moon is certainly a well-defined
and measurable factor, but morality is not scientifically measurable. Thus there is no experiment that can be performed to test the
hypothesis.
Which of the following would be useful as scientific hypotheses?

 Mice require calcium for developing strong bones.
 Dogs are happy when you feed them steak.
 An active volcano can be prevented from erupting by throwing a virgin into it during each full moon.
 The higher the intelligence of an animal, the more easily it can be domesticated.
 HIV (human immunodeficiency virus) can be transmitted by cat fleas.
The investigator devises an experiment or collects data that could prove the hypothesis false. He should also think through the
possible outcomes of the experiment (whether the hypothesis is supported or proven false) and make predictions about the effect
of the manipulated variable on the responding variable in each situation. For example, a scientist has made the following hypothesis:
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“Increasing the amount of fertilizer applied will increase the number of peanuts produced.” He has designed an experiment in which
different amounts of fertilizer are added to plots of land and the number of peanuts yielded per plot is measured. The predictions
should state specifically how the responding variable will change in relation to the manipulated variable and must be stated as an “If
… Then” statement. The general format for an “If … Then” statement is “if the manipulated variable is changed in this way, then the
responding variable will change this way.” For example, if the amount of fertilizer applied to a field is doubled, then the number of
peanuts produced will double. Or, if the temperature of the reactants in a chemical reaction increases, then the rate of the reaction
will increase.
Write a hypothesis for each of the following:

 Guinea pigs are kept at different temperatures for 6 weeks. Percent weight gain is recorded.
 Batches of seeds are soaked in salt solutions of different concentrations and the number of seeds that germinate is
counted for each batch.
Step 7: Setting the Levels of Treatment

Once the investigator has decided what the manipulated variable for an experiment should be, he must also determine how to
change or vary the manipulated variable. The values set for the manipulated variable are called the levels of treatment. For
example, an experiment measuring the effect of fertilizer on peanut yield has five treatments. In each treatment, peanuts are grown
on a 100-m2 plot of ground, and a different amount of fertilizer is applied to each plot. The levels of treatment in this experiment
are set as 200 g, 400 g, 600 g, 800 g, and 1000 g fertilizer/100 m2.
The investigator's judgment in setting levels of treatment is usually based on prior knowledge of the system. For example, if the
purpose of the experiment is to investigate the effect of temperature on weight gain in guinea pigs, the scientist should have enough
knowledge of guinea pigs to use appropriate temperatures. Subjecting the animals to extremely high or low temperatures can kill
them and no useful data would be obtained. Likewise, the scientist attempting to determine how much fertilizer to apply to peanut
fields needs to know something about the amounts typically used so he could vary the treatments around those levels.
Step 8: Identifying the Control Group

It is also necessary to include control groups in an experiment. CONTROL GROUPS ARE DIFFERENT THAN CONTROLLED VARIABLES. A
control group is a treatment in which the manipulated variable is either eliminated or is set at a standard value. The results of the
control group are compared to the results of the experimental treatments. In the fertilizer example, the investigator must be sure
that the peanuts don't grow just as well with no fertilizer at all. The control would be a treatment in which no fertilizer is applied. An
experiment on the effect of temperature on guinea pigs, however, cannot have a "no temperature" treatment. Instead, the scientist
will use a standard temperature as the control and will compare weight gain at other temperatures to weight gain at the standard
temperature.
Tell what an appropriate control group would be for each of the following examples.
 The effect of light intensity on photosynthesis is measured by collecting oxygen produced by a plant.
 The effect of NutraSweet sweetener on tumor development in laboratory rats is investigated.
Step 9: Determining Replication

Another essential aspect of experimental design is replication. Replicating the experiment means that the scientist repeats the
experiment numerous times using exactly the same conditions to see if the results are consistent. Being able to replicate a result
increases our confidence in it. However, we shouldn't expect to get exactly the same answer each time, because a certain amount of
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variation is normal in biological systems. Replicating the experiment lets us see how much variation there is and obtain an average
result from different trials.
A concept related to replication is sample size. It is risky to draw conclusions based upon too few samples. For instance, suppose a
scientist is testing the effects of fertilizer on peanut production. He plants four peanut plants and applies a different amount of
fertilizer to each plant. Two of the plants die.
Can he conclude that the amounts of fertilizer used on those plants were lethal? What other factors might have affected the
results?
In IB Biology, the minimum sample size for each level of treatment of the manipulated variable is 5.
Step 10: Writing the Method

After formulating a hypothesis and selecting the manipulated and responding variables, the investigator must find a method to
measure the responding variable; otherwise, there is no experiment. Methods are learned by reading articles published by other
scientists and by talking to other scientists who are knowledgeable in the field. For example, a scientist who is testing the effect of
fertilizer on peanuts would read about peanut growth and various factors that affect it. He would learn the accepted methods for
evaluating peanut yield. He would also read about different types of fertilizers and their composition, their uses on different soil
types, and methods of application. The scientist might also get in touch with other scientists who study peanuts and fertilizers and
learn about their work. Scientists often do this by attending conferences where other scientists present results of investigations they
have completed.
SI Units and How to Use Them

When you make quantitative observations you are expected to use the appropriate units. The system of units used is the
International System of Units - SI units (Système International d’Unités). In the table below you are given some of the more common
SI units you will need to use.
When measuring time, it is appropriate to use minutes, hours or days if the experiment spans a longer period. When showing
lengths, it is appropriate to use the following units:
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Measurement Precision
o Unless there is a digital display, always measure to one spot beyond the smallest unit of CERTAIN measurement of the tool.
o For example, if you use a ruler that can accurately measure to the tenth of a centimeter, your measurement would be to the
hundredth of a centimeter.
This line would

be measured as
4.50 cm
o You may need to estimate the degree of precision sometimes especially with stop watches. Digital stop watches are said to be
accurate to 0.01s but your reaction time is only 0.1s.
o For electronic probes you may have to go to the manufacturers specifications (on their web site or in the instructions manual).
Measurement Uncertainty
o Every measurement has an uncertainty associated with it, unless it is an exact, counted integer, such as the number of trials
performed.
o The lower the accuracy and precision of a measurement instrument are, the larger the measurement uncertainty is.
o The numerical value of a ± uncertainty value tells you the range of the result. For example a result reported as 1.23 ± 0.05
means that the experimenter has some degree of confidence that the true value falls in between 1.18 and 1.28
o To determine uncertainty:
o Find the smallest increment of measurement on your measurement device
o Divide it by two
o Round to the first non-zero number
This line would be

measured as 4.50 cm
+/- 0.05cm
o Experimental uncertainties should be rounded to one significant figure. Uncertainties are almost always quoted to one
significant digit (example: ±0.05 s).
o Always round the measurement or result to the same decimal place as the uncertainty. It would be confusing to suggest that
you knew the digit in the hundredths (or thousandths) place when you admit that you unsure of the tenths place.
Wrong: 1.237 s ± 0.1 s

Correct: 1.2 s ± 0.1 s
Complete this table as a reference for measuring tools used in the IB Biology class.
MEASURING DEVICE MEASURES UNIT(S) UNCERTAINTY
Gram Scale
Meter Stick Length
Ruler
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MEASURING DEVICE MEASURES UNIT(S) UNCERTAINTY
Stop Watch
Glass thermometer
Metal thermometer
10 graduated cylinder Volume
100 graduated cylinder
15 beaker
250 beaker
1000 beaker
250 flask
1000 flask
4000 flask
Microscopy
Magnifying Power
A compound microscope has two sets of lenses. The lens you look through is called the ocular. The lens near the specimen being
examined is called the objective. The objective lens is one of three or four lenses located on a rotating turret above the stage, and
that vary in magnifying power. The lowest power is called the low power objective (LP), and the highest power is the high power
objective (HP).
You can determine the magnifying power of the combination of the two lenses by multiplying the magnifying power of the ocular by
the magnifying power of the objective that you are using. For example, if the magnifying power of the ocular is 10 (written 10X) and
the magnifying power of an objective is 4 (4X), the magnifying power of that lens combination is 40X.
Field of View (FOV)

The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine
the diameter of your field of view, place a transparent metric ruler under the low power (LP) objective of a microscope. Focus the
microscope on the scale of the ruler, and measure the diameter of the field of vision in millimeters. Record this number.
When you are viewing an object under high power, it is sometimes not possible to determine the field of view directly. The higher
the power of magnification, the smaller the field of view. The diameter of the field of view under high power must be calculated
using the following equation.
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For example, if you determine that your field of view is 2.5 mm in diameter using a 10X ocular and 4X objective, you will be able to
determine what the field of view will be with the high power objective by using the above formula. For this example, we will
designate the high power objective as 40X.
Estimating the Size of the Specimen Under Observation

Objects observed with microscopes are often too small to be measured conveniently in millimeters. Because you are using a scale in
millimeters, it is necessary to convert your measurement to micrometers. Remember that 1 μm = 0.001 mm.
To estimate the size of an object seen with a microscope, first estimate what fraction of the diameter of the field of vision that the
object occupies. Then multiply the diameter you calculated in micrometers by that fraction. For example, if the field of vision’s
diameter is 400 μm and the object’s estimated length is about one-tenth of that diameter, multiply the diameter by one-tenth to
find the object’s length.
SKYLINE MICROSCOPES QUICK REFERENCE SHEET

EYEPIECE MAGNIFICATION OBJECTIVE MAGNIFICATION TOTAL MAGNIFICATION FIELD OF VIEW
10 4 Measured
10 10 Calculated
10 40 Calculated
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Lab Drawings
Drawing Materials. All drawings should be done with a sharp pencil line on white, unlined paper. Diagrams in pen are unacceptable
because they cannot be corrected. Lines are clear and not smudged. There are almost no erasures or stray marks on the paper.
Color can be used carefully to enhance the drawing. Stippling is used instead of shading.
Positioning. Center your drawing on the page. Do not draw in a corner. This will leave plenty of room for the addition of labels.
Size. Make a large, clear drawing; it should occupy at least half a page.
Labels. Use a ruler to draw straight, horizontal lines to the right of the side of the drawing. The labels should form a vertical list. All
labels should be printed (not cursive).
Accuracy. Look carefully at the specimen before you start and try to get the proportions right. Draw what you see; as you see it, not
what you imagine should be there. Avoid making “idealized” drawings. You are not necessarily drawing everything that is seen in
the field of view. Draw only what is asked for. Show only as much as necessary for an understanding of the structure – a small
section shown in detail will often suffice. It is time consuming and unnecessary, for example, to reproduce accurately the entire
contents of a microscopic field.
Do not copy textbook drawings. They are often diagrams! A drawing is different from a diagram. Whilst a drawing is an accurate
record of your observations of a particular biological specimen, showing only the features that can be seen clearly, a diagram is a
more stylized representation of a structure. In a diagram, it is customary to include all the essential features known to be associated
with the specimen, whether visible or not.
Technique. Keep looking back at your specimen whilst you are drawing. If using a microscope, while you are observing increase the
magnification to observe more details and reduce the magnification to get a more general view. Use the focusing controls on the
microscope to observe at different depths of the specimen. Move the specimen around; do not just concentrate on one part.
Observe the general appearance first.
When drawing low power plans do not draw individual cells. Show only the distribution of tissues.
When making high power drawings, draw only a few representative cells; indicate thickness of walls, membranes, etc.
Title. The title should state what has been drawn and what lens power it was drawn under (for example, phrased as: drawn as seen
through 400X magnification). Title is informative, centered, and larger than other text. The title should always include the scientific
name (which is italicized or underlined).
Scale. Include a linear magnification that indicates how many times larger the drawing is compared to life size and a scale line that
indicates relative size. To determine magnification compared to life size, use the equation:
The estimated size of the object

being drawn as determined by
Measurement of
figuring how much of the field of
the scale line you
view it fills.
drew with a ruler.
Actual size of line / size the line represents = magnification
Be sure these numbers are in the same unit of measurement. If not, convert first before calculating linear magnification!
Since you are calculating magnification from an estimate, you must round your answer to one signification digit.
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Graphing
Pie Charts Designed to show a percent of a whole, where the whole equals 100%. Pie charts are
used to compare data, but cannot be used to see how a manipulated variable affects
a responding variable. Pie charts do not show change with respect to another
variable, such as time.
Bar Graphs Designed to make comparisons of data. The data represented in bar graphs are not
necessarily dependent on any other variables and the groupings are usually
qualitative (i.e. grouped into categories, like blood types or color). The bars do NOT
touch.
Histogram Histograms are similar to bar graphs except the data represented in histogram is
usually in groups of continuous numerical (quantitative) data. In this case, the bars
do touch.
Line Graphs A line graph consists of a series of points plotted on the grid and then connected
together by a line or a curve. Line graphs are only used when both variables are
quantitative. Line graphs show trends, such as how things change over time. They are
the best type of graphs to use to show how one factor affects another factor. They
are typically used to express the relationship between responding and manipulated
variables. For example, a line graph would be used to show a baby’s increase in mass
(responding variable) over time (manipulated variable) or how the chances of birth
defects decrease (responding variable) with an increase in a mother’s folic acid intake
(manipulated variable).
Scatter Plot The points are plotted on the grid, but they are usually not joined with a line or a
curve. Line graphs are only used when both variables are quantitative. These graphs
are useful for showing if a relationship exists between two variables, especially when
it is not possible to alter either of the variables (i.e. in descriptive studies).
How do I know which type of graph to use? Follow this key…

1. Is the data a percent that sums to 100%?
a. If yes………………………………………………………………………………….. Pie chart
b. If no…………………………………………………………………………………… Go to #2
2. Are both your manipulated and responding variables quantitative?

a. If no………………………………………………………………………………….. Bar graph
b. If yes …………………………………………………………………………………. Go to #3
3. Are your manipulated variable levels continuous or clumped into groups?

a. Continuous…………………………………………………………………………. Scatter plot/line
b. Clumped ……………………………………………………………………………. Histogram
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Formatting a Graph
Graphing in Excel 2010

1. Open Excel and enter your data in columns. You can label the columns if you prefer.
2. To calculate mean:
a. Click on the box in which you want the mean to be placed
b. Click the formulas tab at the top of the screen
c. Select the “insert function button”
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d. A new box pops up. Search to find the AVERAGE option,
click OK
e. Highlight the data of which you want the average to be
calculated, click OK
3. To calculate standard deviation

a. Click on the box in which you want the SD to be placed
b. Click the formulas tab at the top of the screen
c. Select the “insert function button”
d. Search to find the STDEV option, click OK
e. Highlight the data of which you want the SD to be calculated,
click OK.
NOTE: be sure not to select the mean as one of your data points for calculating standard deviation. This is a
common mistake.
4. To select the correct number of digits for your answer (remember, you want correct precision)
a. Select the top box between row 1 and column A. This should highlight your entire spreadsheet.
b. Click the HOME tab along the top

c. Select format cells and then format cells again.
d. Select number, and indicate the number of decimal places you
want included. Hit OK.
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5. To graph
a. Along the top, select INSERT
b. Highlight the column headings (i.e. boy/girl), hold down the CTRL key and
highlight the means
c. Select the type of graph you want
d. When you click on the graph, a new set of tools opens up, called CHART
TOOLS. From this you can change the chart layout (do this to add a title)
or bar colors.
f. Within your graph, click on and delete the key that says “series 1”
g. To add axis labs, select the LAYOUT tab under CHART TOOLS and choose the “axis titles” option.
6. To add SD error bars

a. Click anywhere on the graph to open the chart tools
b. Click the layout tab
c. Select error bars and then more error bar options.
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d. Select custom and specify value 
e. A little box comes up that looks like:
f. Delete what is in each box

g. For EACH box (positive and negative) highlight the boxes that include the standard deviations. Highlight them all at
once.
h. Hit ok.
Statistics: Percentage Change

A percentage change is a way to express a change in a variable. It represents the relative change between an old value and an new
one.
V1 = the old value, the original value

V2 = the new value
Statistics: Mean
 The sum of all the data points divided by the number of data points.
 Measure of central tendency for normally distributed data.

 DO NOT calculate a mean from values that are already averages.
 DO NOT calculate a mean when the measurement scale is not linear (i.e. pH units are not measured on a linear scale).
Statistics: Standard Deviation

Averages do not tell us everything about a sample. Samples can be very uniform with the data all bunched around the mean or they
can be spread out a long way from the mean. The statistic that measures this spread is called the standard deviation. The wider the
spread of scores, the larger the standard deviation. For data that has a normal distribution, 68% of the data lies within one standard
deviation of the mean.
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MEN WOMEN
HOW TO CALCULATE STANDARD DEVIATION: 0.90 1.50
Calculate the standard deviation by subtracting the mean of a distribution from the value of each
individual variable in the distribution, squaring each resulting difference, summing these squared 2.00 3.00
differences, then dividing this sum by the number of variables, and finally taking the square root of this 1.40 3.00
quotient.
2.00 2.50
1. Calculate the mean (M) of a set of data
3.00 3.00
MEN WOMEN
2.00 3.00
3.00 4.00
4.00 3.00
3.70 2.00
2. Subtract the mean from each point of data to determine (X-M)

MEN WOMEN
2
3. Square each of the resulting numbers to determine (X-M)
MEN WOMEN
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2
4. Add the values from the previous step together to get ∑(X-M)
MEN WOMEN
5. Calculate (n-1) by subtracting 1 from your sample size. Your sample size is the total number of data points you collected.
MEN WOMEN
2 2
6. Divide the answer from ∑(X-M) by the answer from (n-1) to find ∑(X-M)
n-1
MEN WOMEN
7. Calculate the square root of your previous answer to determine the standard deviation
MEN WOMEN
SO WHAT?
On a graph…
If SD error bars overlap…
If SD error bars do not overlap …
Using EXCEL to calculate the mean and the standard deviation

A
Type the values you are trying to find the mean for in a column. You can label the column, but you
don’t have to. 1 Number of Pennies
2 134
Determine which box you want the mean to appear in. In the example, I want the mean to appear
3 130
in box A12. In that box, type: =AVERAGE(A2:A11) and then hit enter. Basically you are telling Excel
to average boxes A2 through A11. 4 136
5 132
Determine which box you want the standard deviation to appear in. In the example, I want the
6 131
standard deviation to appear in box A13. In that box, type: =STDEV(A2:A11) and then hit enter. You
are giving Excel the box labels for the data for which you want to find the standard deviation. 7 137
8 131
Once you have the mean and standard deviation, you need to make sure that you set the values to
9 135
the correct number of digits. EXCEL will default to giving you too many numbers after the decimal
place. Your mean and standard deviation must have the same precision (number of digits after the 10 130
decimal) as your data points. In the example to the right, since the raw data is a whole number, the 11 129
mean and standard deviation must also be whole numbers. The mean would round to 133 and the
12 132.5
standard deviation to +/- 3.
13 2.798809
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Using TI-83/84 to calculate the mean and the standard deviation
First you have to enter the data. Hit the STAT button and you will see the options EDIT, CALC
and TESTS atop the screen. Use the left and right arrows (if necessary) to move the cursor to
EDIT, then select 1: Edit...
Now you will see a table with the headings L1 and L2. Enter the values under L1 (if you want to
clear pre-existing data first, move the cursor to the top of the column, hit CLEAR and then
ENTER.)
Once all the data is entered, go back to the STAT menu, but this time move the cursor to CALC
instead of EDIT.
Once you're in the CALC menu, select 1-Var Stats, then hit ENTER. The calculator will display the
x-mean, some other stuff, and then the standard deviation (sx). Note that sx is what we want!
This is followed by something called sigma x (which is what you would get as standard deviation
if you had used n instead of n-1), and finally the sample size.
Using TI-INSPIRE to calculate the mean and the standard deviation

Create a new list and type your data into columns. You MUST Name your list (arrow to the top
of the column and enter the name of your list). Keep the name descriptive, but short.
Once your data is entered, hit MENU, #4 Statistics, #1 Stat Calculations, and choose #1 One-
Variable Statistics. The choice "Open Variable Statistics" yields the following results back to the
spreadsheet columns. One column will "label" these values, while the following column contains
the actual values.
= mean
sx = the sample standard deviation
Statistics: The Independent T-test
The Student’s t-test is a statistical test that compares the ___________________ and ____________ ________________ of two
samples to see if there is a ______________________________ between them. In an experiment, a t-test might be used to
calculate whether or not differences seen between the control and each experimental group are a factor of the manipulated
variable or simply the result of chance.
Where:
( x1 – x2 ) x1 is the mean of sample 1
s1 is the standard deviation of sample 1
t= n1 is the sample size of sample 1
x2 is the mean of sample 2
= (s1)2 (s2)2 s2 is the standard deviation of sample 2
+ n2 is the sample size in sample 2
n1 n2
There are two hypothesis related to a T-test:
NULL HYPOTHESIS ALTERNATIVE HYPOTHESIS
18
Students in Room 1 Students in Room 2
Student 145 140 138 142 154 148 153 157 161 162
Height (cm) 154 158 160 166 166 162 163 167 172 172
HOW TO CALCULATE T:
1. Calculate the mean (X) of a each sample
Room 1: ____________ Room 2: _____________
2. Find the absolute value of the difference between the means
3. Work out the standard deviation for each sample (use a calculator…)
Room 1: ____________ Room 2: _____________
4. Square the standard deviation for each group
5. Divide each squared standard deviations by the sample size of that group.
6. Add these two values
7. Take the square root of the number
8. Divide the difference in the means (step 2) by the standard error of the difference (step 7)
9. Determine the degrees of freedom (df) for the test. In the t-test, the degrees of freedom is the sum of the
sample sizes of both groups minus 2.
Use the 95%

10. Given the df, look up the critical t-value in a standard table of significance
(p=0.05)
confidence limit
11. Check your answers on-line using the t-test calculator posted at www.biologyforlife.com
19
SO WHAT?
If your calculated t value is _________ than the number in the If your calculated t value is _________ than the number in the
table, you conclude that the difference between the means for table, you conclude that the difference between the means for
the two groups is ________ the two groups is _________________________ different.
_________________________different. Meaning: Meaning: ____________________________
____________________________ ____________________________________
____________________________________
Do not worry if you do not understand how or why the test works ….
Follow the instructions CAREFULLY
T Test Critical Values
20
Performing a T-test with the TI-83/84
1. Hit the STAT button on the calculator

2. Select option 4 to clear any past lists of data.
3. Select option 1 to EDIT your lists.
4. Enter your data for each group as List 1 and List 2
5. Hit STAT button and use the arrow key to move over to the TESTS option
6. Scroll down to option 4, the 2-sample T test and hit ENTER
7. Scroll to the bottom of the screen and hit ENTER over the CALCULATE option
8. Your results are given.
T = calculated T value
df = Degrees of Freedom
X1 = mean of list 1
X2 = mean of list 2
Sx1 = standard deviation of list 1
Sx2 = standard deviation of list 2
Statistics: ANOVA (analysis of variance)

The ANOVA test is a statistical test that can be done in place of multiple T-tests when comparing the means of more than
two groups at a time. The t-test tells us if the variation between two groups is "significant". If you have 5 levels of a
manipulated variable in an experiment, you would need to compare the mean of EACH LEVEL OF THE MV to the mean of
EACH OTHER LEVEL OF THE MV. That’s 10 T-tests!
Multiple t-tests are not the answer because as the number of groups grows, the number of needed pair comparisons
grows quickly. If we did 10 t-tests, we should not be surprised to observe things that happen only 5% of the time. The
ANOVA statistic prevents up from having to do multiple t-tests puts all the data into one number.
NULL HYPOTHESIS: no significant difference exists between more than two means; no single level of the MV
leads to a result different than the other levels of the MV.
ALTERNATIVE HYPOTHESIS: a significant difference exists between means; at least one level of the MV leads to a
result different than the other levels of the MV.
The math required of the ANOVA test is beyond the scope of this class.
There are excellent on-line ANOVA calculators that will do the math and
draw a conclusion for you. The TI 83/84 calculators can also calculate the
ANOVA statistic.
Performing an ANOVA test with the TI-83/84

4. Enter your data for each group as Lists.
The data for each level of the MV should be placed in its own list.
5. Hit STAT button and use the arrow key to move over to the TESTS option
6. Scroll down to option H, the ANOVA and hit ENTER
7.Enter the lists you want to include in the ANOVA
8. Your results are given.
The ANOVA test will result in a “p-value.” If the p-value you get is less
than 0.05, we reject the null hypothesis and conclude that there is a
significant difference between the means being compared. Likewise,
if the p-value you get is more than 0.05, you would accept the null
hypothesis and conclude that there is no significance difference between the means.
21
Statistics: Correlations
One of the most common errors we find in the press is the confusion between correlation and causation in scientific and health-
related studies. In theory, these are easy to distinguish — an action or occurrence can cause another (such as smoking causes lung
cancer), or it can correlate with another (such as smoking is correlated with alcoholism). If one action causes another, then they are
most certainly correlated. But just because two things occur together does not mean that one caused the other, even if it seems to
make sense.
One way to get a general idea about whether or not two variables are related is to plot them on a “scatterplot”. If the dots on the
scatterplot tend to go from the lower left to the upper right it means that as one variable goes up the other variable tends to go up
also. This is a called a “direct (or positive) relationship.” On the other hand, if the dots on the scatterplot tend to go from the upper
left corner to the lower right corner of the scatterplot, it means that as values on one variable go up values on the other variable go
down. This is called an “indirect (or negative) relationship.”
22
Example 1: “girls who watch soap operas are more likely to have eating disorders”
Is there a direct or indirect correlation?
Is it fair to conclude there is a causal relationship?
Example 2: “as ice cream sales increase, the rate of drowning deaths increases sharply.”
Example 3: “people who have more years of education tend to have fewer years in jail”
How can one best determine if there is a causal relationship between two variables?
The most effective way of doing this is through a controlled study. In a controlled study, two
groups of people who are comparable in almost every way are given two different sets of
experiences (such one group watching soap operas and the other game shows), and the
outcome is compared. If the two groups have substantially different outcomes, then the
different experiences may have caused the different outcome.
Statistics: Correlation Coefficient (r)

A really smart guy named Karl Pearson figured out how to calculate a summary number that allows you to answer the question
“How strong is the relationship of a correlation?” In honor of his genius, the statistic was named after him. It is called Pearson’s
Correlation Coefficient (r).
23

2 2
Step 1: calculate and fill in the X and Y values
 Step 2: multiply each X score by its paired Y score which will give you the cross-products of X and Y.
 Step 3: fill in the last row of the table which contains all of you “Sum Of” statements. In other words, just add up all of
2 2
the X scores to get the ΣX, all of the X scores to get the Σ X and etc.
 Step 4: Enter the numbers you have calculated in the spaces where they should go in the formula.
24

2 2
Step 5: Multiply the (ΣX)( ΣY) in the numerator (the top part of the formula) and do the squaring to (ΣX) and (ΣY) in
the denominator (the bottom part of the formula).
 Step 6: Do the division by n parts in the formula.
 Step 7: Do the subtraction parts of the formula
 Step 8: Multiply the numbers in the denominator.
 Step 9: Take the square root of the denominator.
 Step 10: Take the last step and divide the numerator by the denominator and you will get the Correlation Coefficient!
What Good Is A Correlation Coefficient?

As can see above, we just did a whole lot of calculating just to end up with a single number. How ridiculous is that? Seems kind
of like a waste of time, huh? Well, guess again! It is actually very cool! (“Yeah, right!” you say, but let me explain.)
Important Things Correlation Coefficients Tell You

1. They Tell You The Direction Of A Relationship: If your correlation coefficient is a negative number you can tell, just by
looking at it, that there is an indirect, negative relationship between the two variables. As you may recall, a negative
relationship means that as values on one variable increase (go up) the values on the other variable tend to decrease (go
down) in a predictable manner. If your correlation coefficient is a positive number, then you know that you have a direct,
positive relationship. This means that as one variable increases (or decreases) the values of the other variable tend to go in
the same direction. If one increases, so does the other. If one decreases, so does the other in a predictable manner.
25
2. Correlation Coefficients Always Fall Between -1.00 and +1.00:
a. A correlation coefficient of -1.00 tells you that there is a perfect negative relationship between the two variables.
This means that as values on one variable increase there is a perfectly predictable decrease in values on the other
variable. In other words, as one variable goes up, the other goes in the opposite direction (it goes down).
b. A correlation coefficient of +1.00 tells you that there is a perfect positive relationship between the two variables.
This means that as values on one variable increase there is a perfectly predictable increase in values on the other
variable. In other words, as one variable goes up so does the other.
c. A correlation coefficient of 0.00 tells you that there is a zero correlation, or no relationship, between the two
variables. In other words, as one variable changes (goes up or down) you can’t really say anything about what
happens to the other variable.
3. Larger Correlation Coefficients Mean Stronger Relationships

a. Most correlation coefficients (assuming there really is a relationship between the two variables you are examining)
tend to be somewhat lower than plus or minus 1.00 (meaning that they are not perfect relationships) but are
somewhat above 0.00. Remember that a correlation coefficient of 0.00 means that there is no relationship
between your two variables based on the data you are looking at.
b. The closer a correlation coefficient is to 0.00, the weaker the relationship is and the less able you are to tell exactly
what happens to one variable based on knowledge of the other variable. The closer a correlation coefficient
approaches plus or minus 1.00 the stronger the relationship is and the more accurately you are able to predict
what happens to one variable based on the knowledge you have of the other variable.
26
Making Statistical Inferences from Pearson’s r.
How do you determine whether or not your correlation is simply a chance occurrence or if it really is true of the population? You will
need three things in order to determine whether you can infer that the relationship you found in your sample also is true (in other
words, “is generalizable” in the larger population:
1. The Correlation Coefficient that you calculated

2. Something called the “degrees of freedom” which is simply the number of pairs of data in your sample minus 2.
DF =
3. A table of “Critical Values” of the correlation coefficient.
Just like the T-test,

we’ll always use the
Pearson’s R Critical Values 0.05 level of
significance
The first thing you need to do is look down the degrees of freedom column until you see the row with the number of degrees of
freedom that matches your sample degrees of freedom. Look across to the number listed under .05. This number is called “the
critical value of r”.
Critical r =
27
SO WHAT?
If your calculated r value is ____________________________ If your calculated r value is ____________________ than the
the number in the table, you conclude that the correlation is number in the table, you conclude that the correlation is
________ ___________________________________________ ________ ___________________________________________
___________________________________________________ ___________________________________________________
____________________________________________________ ____________________________________________________
Just to make sure that you are getting the idea here, try a few examples.
Performing an Correlation Coefficient test with the TI-83/84

4. Enter your data for each variable as Lists.
5. Hit STAT button and use the arrow key to move over to the
CALC option
6. Scroll down to option 4, the LInReg (ax+b), press 4 and hit
ENTER
7. Press VARS. Scroll down to 5: STATISTICS and press 5 and hit
ENTER.
8. Scroll over to EQ. Then, scroll down to 7:r and press 7. ENTER
again to get the correlation coefficient.
28
Statistics: Chi-Square (X2)
Have you ever wondered why the package of M&Ms you just bought never seems to have enough of your
Brown 13%
favorite color? Or, why is it that you always seem to get the package of mostly brown M&Ms? Is the number
of the different colors of M&Ms in a package really different from one package to the next, or does the Mars Yellow 14%
Company do something to insure that each package gets the correct number of each color of M&M? I’ll bet Red 13%
like me, you’ve stayed up nights wondering about this! Well, I recently visited the M&M web page Blue 24%
(http://www.mms.com/us/) and found that the Mars Company claims that each package of M&Ms they sell Orange 20%
should have the following percentages of each color of M&M: Green 16%
Does the expected percentage of each color of M&M’s in the bag match what we
actually observe in a bag? Let’s do a statistical test to find out!!
Chi-square Goodness of Fit is a statistical test commonly used to compare observed data with data we would expect to
obtain. Were the deviations (differences between observed and expected) the result of chance, or were they due to
other factors?
There are two hypothesis related to a X2-test:
NULL HYPOTHESIS ALTERNATIVE HYPOTHESIS
The formula for calculating chi-square is
1. The first step in the Chi Square Calculation Table for M&M Colors
calculation of an X2 M&M Color Observed Values Expected Values (O-E)2 / E
value is to determine Brown
the expected Yellow
numbers.
Red
2. Then, use the Blue
following formula for Orange
each observed and
expected category: Green
Total 100% 100%

(O-E)2 / E
3. The results are added together to get a final X2 value.
29
4. The calculated X2 value is than compared to the ____________________________ found in an X 2
distribution table. The X2 distribution table represents a theoretical curve of expected results. The
expected results are based on___________________________.
Degrees of Freedom = ________________________________.
The X2 distribution table is organized by the Level of Significance. The level of significance is the maximum
tolerable probability of accepting a false null hypothesis. We always use 0.05. This means that we would
accept the null hypothesis if the observed results were within _______of the expected results.
Number of classes (n) = ______
df = _______________________
5. If our calculated value is ______________than the .05 level of significance, we can accept our null
hypothesis.
If our calculated value is ______________ than the .05 level of significance, we can reject our null
hypothesis.
The X2 obtained was _______________. The critical value X2 (found in the distribution table) was
___________. The calculated value is ______________ than the .05 level of significance, so we can
________________ the null hypothesis. In other words, the actual M&M’s in the bag were ________
___________________________________ than what we expected to see in the bag.
Performing an Chi-Square test with the TI-83/84

nd
1. Press [2 MATRIX]
2. Select [EDIT - > 1:A]
3. Copy the data by typing in each number and then pressing ENTER
4. Now press STAT. Under the TESTS sub-menu, scroll down and select C:X2 TEST. Press ENTER.
5. Move the cursor down to DRAW and press ENTER.
The Chi-Square test will result in a “p-value.” If the p-value you get is less than 0.05, we reject the null hypothesis and conclude that
there is a significant difference between the observed and expected values. Likewise, if the p-value you get is more than 0.05, you
would accept the null hypothesis and conclude that there is no significance difference between the observed and expected.
30
Just like the T-test, we’ll
Chi Square Critical Values always use the 0.05 level
of significance
31
Report Writing
The purpose of writing a lab report is to determine how well you performed your investigation, how much you understood what
happened during the process, and how well you can convey that information in an organized fashion. Remember that lab reports
are individual assignments. You may have had a lab partner, but the work that you do and report on should be your own. Use the
following “tool kit” as a guide when you write up your formal lab reports.
LOGISTICS:
 Raw data must be collected in a bound lab composition book
 Lab must be typed (with 1.5 line spacing) or neatly hand written
 Title of lab is clear and relevant
 Report has a logical order, with clear title and section headings
 The spelling, grammar, and flow of the writing must be correct.
 When you write a lab report, you will have already performed the investigation. Please use the past tense throughout the
paper.
DESIGN ASPECT 1: Defining the Problem and Selecting Variables
 Include a Background Information section. Introduce and explain the biological principles and/or concepts that are being
investigated. Remember to cite your sources of information properly.
 Provide the scientific name of the organism being investigated (Genus species).
 State the PROBLEM QUESTION (PQ). Be sure your problem question is focused enough so that it specifically states what
was under investigation in the experiment. If a controlled experiment was done, the manipulated and responding variables
must be clearly identified. Often, but not always, written as, “What is the effect of __MV__ on __RV__?”
There are two main types of investigations that you will perform in IB biology:
1. Experiments are studies that allow scientists to manipulate a variable and observe its effects. For example:
Does changing light affect the growth of radishes? Experiments are powerful studies because they can
establish whether a variable influences or determines an outcome.
2. Sometimes experiments are neither possible nor desirable. Human subjects, for example, are often unsuitable
for experimentation for ethical reasons. Jane Goodall, wishing to discover the behavior and social structure of
chimpanzees in their natural habitat, did not perform experiments with her subjects but instead observed
them with minimal human interference. When subjects are studied “as is” rather than manipulated in
controlled settings, they are part of descriptive studies.
 In the case of a true experiment, you need to explain what you changed between groups, the MANIPULATED VARIABLE
(MV). Indicate the manipulated variable and list the levels of the MV that you included in your experimental protocol.
Provide the unit for your MV. Typically you should have a minimum of 5 levels of the MV. Explain how the range of levels
of your MV was selected.
 If you performed a descriptive study, explain why no variable was or could be manipulated.
 If appropriate, indicate your CONTROL GROUP (CG). The control group is the one group to which all other groups will be
compared. The control group receives the exact treatment as the experimental groups except it does not receive the
change in the MV. The control group can be a level of the MV.
Please note that the control group is NOT the same thing as a controlled variable.
For some experiments, there is no control group and the comparison among the experimental groups is enough.
32
 You need to explain what was measured, the RESPONDING VARIABLE (RV). List what was measured (both qualitative and
quantitative data) and explain how it was measured. Provide the unit for your RV. If no qualitative data was collected, say
so, and explain why qualitative data was not gathered.
 For true experiments in which you are determining the effect of a MV on and RV, you need to include a HYPOTHESIS. A
hypothesis predicts a relationship or trend. It will often take the form of a proposed relationship between two or more
variables that can be tested by experiment. Hypothesis statements are often written as:
 If ___(MV)____ is related to ____(RV)___, then (predict the effect).

 If the ___(MV)____ is (described the changes), then the ____(RV)___ will (predict the effect).
 ___(RV)____ will (predict the effect) when ____(MV)___ (describe the changes).
 You must also provide an EXPLANATION for your hypothesis. This should be a brief discussion (paragraph form) about the
science behind your hypothesis and prediction. You should site credible references that support your explanation (see
section on citations).
DESIGN ASPECT 2: Controlling Variables
 At least three CONTROLLED VARIABLES (CV) are required, but more may be necessary. The controlled variables you list
must be relevant to your investigation. You need to control for all variables that may reasonably affect the outcome of the
investigation. Materials used and measurement techniques are NOT controlled variables (they are validity measures).
While materials and techniques must be consistent, a true variable is something that could directly influence the
responding variable, not just how it is measured.
 You must explain why and how variables were controlled. When explaining why a variable needs to be controlled, describe
how the variable could impact the results if it was not controlled. Often times, students create a table to organize this
information:
CONTROLLED VARIABLES WHY in must be controlled HOW it was controlled

1.
2.
3.
DESIGN ASPECT 3: Developing a Method for Collection of Data
Before performing and writing about your experiment, you may need to run some PRE-TRIALS. A pretrial is a pretest
of the experimental methods that you conduct to tweak the procedure before actual data collection begins. Pre-trials
will help you determine specific details of your experiment and increase your change of success with your
experimental design because you can work out any kinks before collecting data.
 Make a list of the MATERIALS needed in the investigation. Be as specific as possible (example: ’50 mL beaker’ instead of
‘beaker’); include the volumes of tubes and cylinders, the concentrations of solutions, the model and manufacturer of any
complex apparatus. If you have to decide how much of a substance or a solution to use, state your reasoning or show the
calculations.
 Include DIAGRAMS OR PHOTOGRAPHS. Be sure your diagram includes a title and any necessary labels. Photographs are
an excellent way to document your experiment. Consider taking the following photographs:
o You, the researcher, shown working on your experiment

o Experimental setup, to show the overall environment
o Individual photos of the experimental and control groups throughout your experiment
33
o Close-ups of how data were collect (for example, a close-up photograph of your hands holding the instrument to take
measurements)
 State or discuss the PROCEDURE that you used in the experiment. Be sure your procedure explains how you changed the
manipulated variable. This can be in paragraph form or a list of step-by-step directions. Provide enough detail so that
another person could repeat your work by reading your report.
o If you use a known, published protocol than you must provide a full citation as a reference.
o Your procedure must include at least three clear VALIDITY MEASURES (VM) (i.e. cleaning test tubes prior to use,
cleaning the microscope lenses, using the same ruler, etc). Validity measures are things kept constant to make sure
experimental measurements are valid and consistent.
o Your procedure must CLEARLY STATE HOW YOU COLLECTED DATA. What measuring device did you use, what data did
you record, when did you collect data? What qualitative observations did you look for?
o Explain how you set up so you had MULTIPLE TRIALS of data collection. The procedure must allow collection of
“sufficient relevant data”. The definition of “sufficient relevant data” depends on the context. The planned
investigation should anticipate the collection of enough data so that the problem question can be suitably addressed
and an evaluation of the reliability of the data can be made. As a rule, the lower limit is a sample size of five. Very
small samples run from 5 to 20, small samples run from 20 to 30, and big samples run from 30 upwards. Obviously, this
will vary within the limits of the time available for an investigation.
o If you will be COMBINING DATA with data collected by other students in the class, you should indicate that, “pooling
data was done to ensure collection of significant, relevant data” (IB Biology subject guide, 2009, page 26). Be sure to
cite this reference if you pool data.
o If you are SAMPLING only a portion of a population, you must explain how and why you ensured that the sample was
randomly selected.
o Your procedure must be safe and ethical. Organisms, including humans, cannot be subject to harm in your
investigation. List any SAFETY PRECAUTIONS that were taken during the lab. If necessary, address the IBO animal
experimentation policy.
DATA COLLECTION AND PROCESSING ASPECT 1: Recording Raw Data
 Create a formal DATA TABLE in which to present the raw, unmodified data you collected. Be sure your table:
o Is easy to understand
o Has a specific title
o Is titled in sequential order as “Table 1: title.” “Table 2: title”
o Has column headings
o Includes the unit of measurement of the MV and RV (always in metric units)
o Includes the measurement uncertainty of the measurement tools used (or, if the data was a count, indicates that
“counts have no measurable uncertainty”). Uncertainly is usually stated in a column heading or as a footnote at
the bottom of the table. Additional information about measurement uncertainty can be found later in this
booklet.
o Has a consistent and correct number of digits for each measurement
o Has decimal points aligning down a column (if applicable) and numbers centered in the column
o Indicates which data was collected by which student IF the data was collected and pooled across multiple students.
34
 Your report must include QUALITATIVE DATA. This might be a paragraph in which you describe:
o Observations about the procedure or deviations from the procedure
o Observations about results not directly relating to the RV
o Specific qualitative data for each trial
 LAB DRAWINGS are considered data by the IB Organization. Biological drawings can be made from biological specimens
viewed with a microscope, binocular microscope or hand-lens. They should always be accurate records of the observed
features of a specimen. Not all labs will include a lab drawing. Additional information about lab drawings can be found later
in this handbook.
DATA COLLECTION AND PROCESSING ASPECT 2: Processing Raw Data
 STATISTICS are useful mathematical tools which are used to analyze data. Common statistics used in biology are:
o Mean
o Range
o Median
o Percent change
o Standard deviation (to determine amount of variation around a mean)
o T-test (to compare two means to determine if they are statistically different from each other). Chi-square (to
determine if “observed” results are significantly different from “expected” results)
o Correlation coefficient (to determine the extent two variables are related to each other).
o Chi-square (to compare observed with theoretically expected results)
Use only the statistical tests appropriate to investigate and address your problem question. Additional information about
statistics can be found later in this booklet.
 When a t-test, chi-square or correlation coefficient is calculated, you must indicate the significance level at which your
critical value is determined (we typically use the 95% confidence interval, 0.05).
 For each statistic you calculate, you must EXPLAIN WHY YOU ELECTED TO DO THAT CALCULATION. What does the
calculation tell you about the data?
35
DATA COLLECTION AND PROCESSING ASPECT 3: Presenting Processed Data
 Show an EXAMPLE CALCULATION for each statistic you calculate. Use plenty of room; make sure they are labeled, are clear
and are legible. Show the units of measurements in all calculations. Pay attention to the number of digits! Don’t lose
accuracy by carelessly rounding off. Do not truncate.
 Present your data processing results in a TABLE. The initial raw data and the processed (calculated) data may be shown in
one table provided they are clearly distinguishable. Be sure your processed data table:
o Is easy to understand
o Has a specific title
o Has column headings
o Includes the unit of measurement
o Has a consistent and correct number of digits for each measurement (to the same precision as your raw data)
o Has decimal points aligning down a column (if applicable) and numbers centered in the column
 You must also present your results in a GRAPH.

o Use the correct type of graph for the type of data you are presenting.
o Graphs must be clear and easy to understand. Please avoid “creative” or “funny” coloring of graphs.
o Graphs need to have appropriate scales, labeled axes with units, and accurately plotted data points.
o Graphs are titled in sequential order as “Figure 1: title.” “Figure 2: title”
o If necessary, add smooth lines or curves to show the overall trend of the data.
o If a mean is calculated, only graph the mean, not all data points. When a mean is graphed, its associated standard
deviation error bar must also be included (and labeled as such).
o Legends (keys) are not always necessary. Delete “series 1” and “series 2” boxes from graphs created in Excel.
CONCLUSION AND EVALUATION ASPECT 1: Concluding
 Write one (or more) paragraphs in which you DRAW CONCLUSIONS FROM YOUR RESULTS. Your conclusion should be
clearly related to the research question and the purpose of the experiment.
o Answer the problem question
o Be accurate (i.e., if you used a T-test, be sure your conclusion matches what the T-test tells you; don’t say there is a
difference if the T-test says the difference is insignificant).
o Was your hypothesis supported or refuted? Use the appropriate language, i.e. “Supports my hypothesis” (not
‘proves’ or ‘is correct’). The word prove is much too strong for a single study on a topic.
o Provide a brief explanation as to how you came to this conclusion from your results. In other words, sum up the
evidence and explain observations, trends or patterns revealed by the data. Summarize the processed data:
mean, range and standard deviation. Refer directly to tables and graphs by referencing tables and figures (i.e. “as
seen in Figure 1…”)
o Summarize the results of the statistical test(s) was the effect of the MV significant or not?
 If possible, CITE LITERATURE related to your conclusion. Does you result coincide with published results? Does it refute
published results?
CONCLUSION AND EVALUATION ASPECT 2: EVALUATING PROCEDURE
 In general, how much CONFIDENCE do you have in the results? Avoid giving your confidence as a percentage; use words
such as “very” or “somewhat.” Are your results fairly conclusive, or are other interpretations/results possible?
 Why are you (or aren’t you) confident? What did you do to make sure your results are valid? Was the range of the MV
levels appropriate? Was the data collected relevant to the problem question?
 Explain any anomalous data points
 Identify and discuss significant ERRORS that actually affected your data collection. You must identify the source of error
and tie it to how it likely affected your results. Avoid hypothetical errors (“could have” or “I might have”) without evidence
to back it up. Common errors include:
36
o Human error: Human error can occur when tools or instruments are used or read incorrectly. Human errors can be
systematic because the experimenter does not know how to use the apparatus properly or they can be random
because the power of concentration of the experimenter is fading. Automated measuring using a data-logger
system can help reduce the likelihood of this error; alternatively you can take a break from measuring from time to
time. Do not list time constraints or time management as errors - they should be eliminated with good practical
skills. The focus here should be on the investigation.
o Calibration error: Some instruments need calibrating before you use them. If this is done incorrectly it can
increase the risk of systematic error.
o Random errors: In biological investigations, the changes in the material used or the conditions in which they are
carried out can cause a lot of errors. Biological material is notably variable.
o The act of measuring: Could the measurement uncertainty have affected the results? Why or why not?
o Uncontrolled variables: What variables were not controlled? What effect might each of these uncontrolled
variables have had on your data? On the conclusion?
o Systematic errors: could the measurement uncertainty have affected the results? Why or why not? Did
systematic errors affect the data? The conclusion?
Errors and their effect on the results can be clearly presented in a table.
 What are the LIMITATIONS of your conclusion? Can the results be generalized to other situations/conditions? How might
your results explain a process in the “real world”?
CONCLUSION AND EVALUATION ASPECT 3: IMPROVING THE INVESTIGATION
 What could you do to make IMPROVEMENTS to the investigation? Suggestions for improvements should be based on the
weaknesses and limitations identified in aspect 2.
 As appropriate, address modifications to the experimental technique and the data range. Suggest future experiments.
 Propose only realistic and specific modifications. “More time” and “be more careful” are inadequate.
REFERENCES AND CITATIONS
 IN TEXT CITATIONS
It is permissible in the design and conclusion sections to use brief quotations. Sometimes a book or reference has a phrase
or sentence that expresses exactly the thought you are trying to convey; you may use that phrase or sentence IF you use
quotation marks and cite a reference at the end of the sentence. It is NOT appropriate to borrow extensive passages (more
than two sentences) from a text or web site.
You should also acknowledge where ideas or knowledge not originally your own come from, even if you state your
understanding of the idea in your own words. This is usually done by putting the first author’s last name and the date of the
paper in parentheses at the end of the sentence containing the idea.
 WORKS CITED LIST
Any source you mention in the text of your paper should be included in a list of references in a separate section at the end
of the paper. These references are usually listed in alphabetical order by the first author’s last name. Make sure all the
authors of a paper or book are listed, and include the title of the book or article, the journal or publisher (and place), and
37
the date. If you used just part of a book, indicate the chapter or pages used. For web sites, give the exact electronic address
and any other information you have about it (the author, the name of the organization that sponsors the site).
Examples:
o Book:
 Author(s). Year. Title. Location: Publisher. Number of pages, or pages cited.
 Hille, Bertil. 1992. Ionic Channels of Excitable Membranes. Second Edition. Sunderland, MA: Sinauer
Associates, Inc. 607p.
o Article:
 Author(s). Year. Title of Article. Journal, volume number, pages.
 Huxley, A.F. and R. Stämpfli. 1949. Evidence for salutatory conduction in peripheral myelinated nerve
fibres. J Physiol. (Lond.) 108: 315-339.
o Web page:
 Name of web page. Creator or publisher. Date accessed. Web address.
 The Animated Brain. Brainviews, Ltd. Saltatory conduction. July, 2012.
http://www.brainviews.com/abFiles/AniSalt.htm
o Lecture or information from a teacher.
 Name of teacher (alphabetically, by last name). The exact date and topic of the lecture (including the
course in which it was given).
 Or for individual answers to questions you asked a teacher, you can call it “personal communication” and
give the date.
 Do not use Wikipedia as a resource site; however you may read it to gain understanding.
38
IB LAB SCORING CHECKLIST:
FORMAT EXPLANATION OF SCORING:
 Raw data collected directly into lab composition book
 Formal report is typed with 1.5 line spacing  If no mark is made next to the box,
 Logical order of report, with clear title and headings it means that the full point was
 Clear spelling, grammar, and flow of the writing awarded for the indicated criteria.
 Sources have in correctly formatted in-text citations (last name, date) The work presented was accurate,
 References correctly cited in an alphabetical ”works cited” list thorough and detailed.
 Table(s) labeled sequentially as Tables (i.e. as Table 1… Table 2…)
 Picture(s), diagram(s) and graph(s) labeled sequentially as Figures  If a slash mark is made across the
 If appropriate, scientific name of organism is provided; italics or underlined box, it means that ½ of a point was
awarded for the indicated criteria.
CONTEXTUAL INFORMATION The work is either incomplete or
 Brief (1-2 sentence) introduction to the topic of the report and how it fits with what is lacks detail.
being learned in class
 Detailed and accurate biology background information relevant to the problem  If a circle is made around the box,
question is included (1-2 paragraphs) it means that no point was
 Hypothesis concerns effect of MV on RV awarded for the indicated criteria.
 Hypothesis explanation is valid The work was either missing or
 Source(s) of background and hypothesis explanation information is cited incorrect.
MANIPULATIVE SKILLS
 Follow directions carefully
 Do not fabricate data
 Seek assistance when appropriate (independence is encouraged)
 Consistently carry out proper safety measures
 Properly use a wide range of experimental equipment
 Safely dispose and reduce waste
 Work in the lab in a way that does not put yourself or others in harm’s way
 Follow the IBO animal experimentation policy
 Maintain a clean and organized lab station and work area
IB DESIGN ASPECT 1: problem and variables

 Problem question is stated with a clear and measurable MV and RV
 Problem question relates to the prompt given in class
 Manipulated variable correctly identified (or if descriptive lab, describe why no MV)
 At least 5 levels of the MV are listed (with unit)
 There is an explanation of how the range of MV levels was selected
 Responding variable is correctly identified
 Quantitative RV provided (with unit)
 Qualitative RV provided (or explain why none)
IB DESIGN ASPECT 2: Control of Variables

 1st of at least 3 relevant controlled variables (which are not validity measures) is identified (CV-1)
 2nd of at least 3 relevant controlled variables (which are not validity measures) is identified (CV-2)
 3rd of at least 3 relevant controlled variables (which are not validity measures) is identified (CV-3)
 There is a clear explanation how the 1st of 3 relevant controlled variables was controlled This info can be included in
 There is a clear explanation how the 2nd of 3 relevant controlled variables was controlled the procedure or in a
 There is a clear explanation how the 3rd of 3 relevant controlled variables was controlled separate section
 There is a clear biological explanation why the 1st of 3 relevant controlled variables was controlled
 There is a clear biological explanation why the 2nd of 3 relevant controlled variables was controlled
 There is a clear biological explanation why the 3rd of 3 relevant controlled variables was controlled
 If possible, data is collected to show that the 1st of 3 relevant controlled variables was controlled
This data can be
 If possible, data is collected to show that the 2nd of 3 relevant controlled variables was controlled
presented later in the lab
 If possible, data is collected to show that the 3rd of 3 relevant controlled variables was controlled report, alongside MV
and RV data
39
IB DESIGN ASPECT 3: Method for Collection of Data
 The name and quantities of major apparatus used are listed, including size and graduation
 The reasoning behind the quantities of materials is given
 There is a titled and labeled picture or diagram of the set up (“Diagram” is not a good title)
 Procedure includes at least 3 validity measures (VM)
 Procedure describes how the MV was varied (MV) between 5 levels
 Procedure describes how data related to the MV was collected
 An adequately broad MV range is considered
 Sample size is at least 5 for each level of the MV (MT)
 Explanation of how and why you sampled data (if appropriate)
 Indication that data was pooled, with reference to IB subject guide (if appropriate)
 Directions about how to collect measurement data (with tool listed) (DC)
 Procedure worked! Sufficient data was collected using the procedure described.
 Reasonable safety precautions are provided
IB DATA ASPECT 1: Collecting and Recording Raw Data

 Data for the RV is independently collected
 Quantitative data for the MV is independently collected
 Quantitative data is collected for at least 5 levels of the MV
 Quantitative data is collected for at least 5 trials of each level of the MV
 Data table is neatly constructed, organized, and makes good use of space. Table does not break across a page.
 Specific title is included. The title indicates what data was collected. Note: table title is NOT the lab title.
 Logical set-up of columns and rows (i.e., MV ordered in first column, RV(s) in next column(s)) with headings at the top of each
column
 Metric units for each piece of data are clearly and correctly identified (may be done at top of each column)
 Measurement uncertainty for both MV and RV is noted and correct (may be done at top of each column)
 Data is measured to a consistent, correct precision for the tool used; decimal points align
 Qualitative data is presented clearly
 If data was pooled between students, data collected by the author is clearly indicated
IB DATA ASPECT 2: Processing Raw Data

 If appropriate, correct difference is calculated for each data point
 If appropriate, correct rate is calculated for each data point
 If appropriate, correct mean is calculated for each level of the MV
 If appropriate, correct standard deviation is calculated for each level of the MV
 If appropriate, correct t-value(s) calculated (if in excel, correct P value is given)
 If appropriate, correct ANOVA calculated (correct P value is given)
 If appropriate, correct correlation coefficient is calculated
 If appropriate, correct slope is calculated
 If appropriate, correct % error is calculated
 There is an explanation for EACH data processing calculation (How will the processing of the data help answer the problem
question? What will the processed data “tell you”?)
IB DATA ASPECT 3: Processed Data Presentation

 Working for processed data has been presented so that all the stages to the final result can be followed
 Processed data is organized into a easy to read table, with clearly labeled title and column headings (with units)
 Processed data has correct precision; decimal points align
 If appropriate, significance level (0.05), critical t-values and degrees of freedom are provided
 The correct type of graph is made for the type of data presented (i.e. bar, line, histogram, pie, etc…)
 Graph is neatly constructed, organized, and makes good use of space. If used, colors make the graph more readable.
 Specific title is included. The title indicates what data is presented. Graph title is NOT the lab title.
 RV on the Y axis and MV on the X axis
 Units are clearly and correctly identified along the X and Y axis
 The graph axes are proportional to the data
 The numbers on the graph have a consistent and correct precision
40
 All points are plotted clearly and correctly. The mean of the data is graphed (not each individual trial).
 Graph includes SD error bars, labeled as such
 If needed, a best fit line or smooth curves is added to the graph to show trends or relationships in the data
 “Series” boxes are deleted from graphs created in Excel
IB CONCLUSION ASPECT 1: Concluding

 Correct conclusion is drawn; conclusion relates to the problem / purpose
 If a hypothesis is being tested, there is a statement whether the data supports or refutes the hypothesis (avoid “proves”)
 Use data to support conclusion (mean, SD and T) with direct reference to figures and tables
 Interpret results of data processing (mean, standard deviation, T-test, ANOVA, correlation, slope, % error)
 Draw a conclusion based on statistical significance
 Compare different graphs, or describe the trends shown in graphs
 If appropriate, compare experimental results with that in a reliable and valid published source (cited appropriately)
 Provide a biological explanation for the results
IB CONCLUSION ASPECT 2: Evaluating Procedure

 Confidence in results is stated
 Positive aspects of lab design are mentioned
 Appropriateness or limitations due to MV range and levels
 Anomalous data points explained (if appropriate)
 Implications of the uncertainties or standard deviations on the results and/or conclusion are explained
st
 1 of at least 3 errors / limitations that actually affected data and/or conclusion (E)
nd
rd
st
 Description of how 1 of 3 errors / limitations may have impacted results and/or conclusion
nd
rd
IB CONCLUSION ASPECT 3: Improving the Investigation

st
 1 of at least 3 improvements to procedure are provided, based in errors / limitations described
nd
rd
 Suggestions are not vague (“be more careful” is unacceptable)
 Suggestions reduce errors, reduce uncertainty, or improve control of variables
 Suggest modifications to MV range
41
IB INTERNAL ASSESSMENT: EXPERIMENTAL DESIGN SCORE
Defining the problem Developing a method
LEVEL Controlling variables
and selecting variables for collection of data
Formulates a focused problem / Designs a method for the effective Develops a method that allows for the
Complete research question and identifies the control of the variables. collection of sufficient relevant data.
2 relevant variables.
Formulates a problem / research Designs a method that makes some Develops a method that allows for the
Partial question that is incomplete or attempt to control the variables collection of insufficient relevant data.
1 identifies only some relevant
variables.
Does not identify a problem / Designs a method that does not Develops a method that does not
Not at all research question and does not control the variables. allow for any relevant data to be
0 identify any relevant variables. collected.
IB INTERNAL ASSESSMENT: EXPERIMENTAL DATA COLLECTION AND PROCESSING SCORE

LEVEL Recording raw data Processing raw data Presenting processed data
Records appropriate quantitative and Processes the quantitative raw data Presents processed data appropriately
Complete associated qualitative raw data, correctly. and, where relevant, includes errors
2 including units and uncertainties and uncertainties.
where relevant.
Records appropriate quantitative and Processes quantitative raw data, but Presents processed data appropriately,
Partial associated qualitative raw data, but with some mistakes and/or omissions. but with some mistakes and/or
1 with some mistakes or omissions. omissions.
Does not record any appropriate No processing of quantitative raw data Presents processed data
Not at all quantitative raw data or raw data is is carried out or major mistakes are inappropriately or incomprehensibly.
0 incomprehensible. made in processing.
IB INTERNAL ASSESSMENT: EXPERIMENTAL CONCLUSION SCORE
LEVEL Concluding Evaluating Procedure(s) Improving the investigation
States a conclusion, with justification, Evaluates weaknesses and limitations. Suggests realistic improvements in
Complete based on a reasonable interpretation respect of identified weaknesses and
2 of the data. limitations.
States a conclusion based on a Identifies some weaknesses and Suggests only superficial improvements.
Partial reasonable interpretation of the data. limitations, but the evaluation is weak
1 or missing.
States no conclusion or the Identifies irrelevant weaknesses and Suggests unrealistic improvements.
Not at all conclusion is based on an limitations.
0 unreasonable interpretation of
the data.
42

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LAB SAFETY SKILLS

NOTIFY YOUR INSTRUCTOR OF ANY ACCIDENT OR POTENTIALLY HAZARDOUS SITUATION.

IF ANY CHEMICAL IS SPLASHED ON YOU SKIN OR IN YOUR EYES,

Step 2: Defining the Problem

Which of the following do you think can be answered by scientific inquiry.

How did you decide what questions can be answered scientifically?

Step 3: Identifying the Responding (Dependent) Variable

Step 4: Identifying the Manipulated (Independent) Variable

Why is the scientist limited to one manipulated variable per experiment?

Identify the manipulated and responding variables in the following examples.

 Height of bean plants is recorded daily for 2 weeks.

Step 5: Identifying the Controlled Variables

What other variables would have to be standardized in this experiment?

List three validity measures that could be used in this experiment.

Step 6: Writing the Hypothesis

Which of the following would be useful as scientific hypotheses?

 Dogs are happy when you feed them steak.

 HIV (human immunodeficiency virus) can be transmitted by cat fleas.

Write a hypothesis for each of the following:

Step 7: Setting the Levels of Treatment

Step 8: Identifying the Control Group

 The effect of NutraSweet sweetener on tumor development in laboratory rats is investigated.

Step 9: Determining Replication

Step 10: Writing the Method

SI Units and How to Use Them

This line would

This line would be

Wrong: 1.237 s ± 0.1 s

Meter Stick Length

10 graduated cylinder Volume

100 graduated cylinder

500 graduated cylinder

2000 graduated cylinder

Field of View (FOV)

Estimating the Size of the Specimen Under Observation

SKYLINE MICROSCOPES QUICK REFERENCE SHEET

The estimated size of the object

Actual size of line / size the line represents = magnification

How do I know which type of graph to use? Follow this key…

2. Are both your manipulated and responding variables quantitative?

3. Are your manipulated variable levels continuous or clumped into groups?

Graphing in Excel 2010

3. To calculate standard deviation

b. Click the HOME tab along the top

6. To add SD error bars

f. Delete what is in each box

Statistics: Percentage Change

V1 = the old value, the original value

 Measure of central tendency for normally distributed data.

Statistics: Standard Deviation

2. Subtract the mean from each point of data to determine (X-M)

If SD error bars do not overlap …

Using EXCEL to calculate the mean and the standard deviation

Using TI-INSPIRE to calculate the mean and the standard deviation

Statistics: The Independent T-test

NULL HYPOTHESIS ALTERNATIVE HYPOTHESIS

Room 1: ____________ Room 2: _____________

2. Find the absolute value of the difference between the means

Room 1: Room 2: _

Room 1: Room 2: _

 If _(MV) is related to (RV)_, then (predict the effect).