Pauline Grace G. Manzanillo CHEM 121.

1 ANALYTICAL CHEMISTRY 1
BS CHEMISTRY 2-A Assignment No. 1- Safety Culture

1. Discuss the difference between good laboratory practice and laboratory safety rule. Give at least 5
examples each
Working in a laboratory involves essential practices and rules that must be followed in all
experiments and other laboratory concerns. Know how to effectively follow good laboratory practices
and safety standards will produce in an appropriate system such as a good flow on conducting
laboratory experiments and prevention of any dangerous situations brought about by incorrect
handling of equipment and chemical components. To have a suitable commitment within the
perimeter of working station upholding the personnel and experimentalists, the distinction between
good laboratory practices and safety rules were established. Good laboratory practices embody a set
of principles that assure the laboratory's reliability and quality. This also refers to the safety
precautions that must be taken in order to maintain structure and unity inside the laboratory. All
laboratory personnel must be accountable for implementing these procedures and must be constantly
vigilant to avoid the development of risks. Wearing personal protective clothing such as laboratory
gowns, aprons, goggles, and gloves are examples of laboratory practices, also, never wear jewelry or
rough garments inside the laboratory. Always put or add labels on chemical containers, especially if
they contain hazardous components. Check the condition of all equipment on a regular basis, and
never use any that has cracks or is damaged. Assign a proper storage in storing chemicals; instead,
waft the odor towards you and preserve a sufficient distance. Always add acid to the water when
combining concentrated acid and water. Conversely, laboratory safety standards govern the safety of
everything contained within the laboratory, including not just the personnel but also the substances
and equipment to be utilized. These guidelines must be followed in order to significantly reduce
hazards and preserve safety within the laboratory.  Examples of basic laboratory rules include: never
trying to work alone in the laboratory and only continue to work under the supervision of an
instructor, avoiding contamination of reagents, understand how and where to properly dispose of
chemicals, do not smell the scent of a certain chemical with your nose, instead wafting the odor
towards you and preserving a proper distance, and never taste or touch the chemicals if not told to do
so.
2. Can you think of materials or things that have hazardous properties that are very useful to us but
can present a hazard to us if we are careless with them or mishandle or misuse them? Enumerate
at least 5 materials and explain its accompanying hazards.

Chemicals typically have their own structure and components. If hazardous substances are
not properly kept or handled, they might cause injury to humans. Even though the product is
beneficial in our daily lives, the presence of hazardous chemicals poses a danger. These are the
following examples of materials with its accompanied hazards:
 Fire is the most prevalent dangerous substance that we encounter in our daily lives. This
substance aids us in the preparation of food, the generation of heat, and the like.
However, because of its corrosive components, fire may endanger us, especially if it is
not monitored. This may be the cause of the expansion of the possibility of uncontrolled
fire, which would have an impact on the resources and resulted in massive destruction.
 Hydrochloric acid, also known as muriatic acid, was commonly used to clean sinks,
toilets, and other surfaces. Because of its corrosive components, it can cause a variety of
health problems, including skin irritation, respiratory tract irritation and inflammation,
 and pulmonary edema if not handled properly. This disease may have arisen as a result of
a person's prolong exposure to this hazardous substance.
 Gasoline makes our automobiles and other modes of mobility more functional. However,
dealing with gasolines or prolonged exposure to them can be harmful to the body,
causing nasal irritation, dizziness, inflamation, and other symptoms. This might be
disastrous if the area is engulfed in flames.
 Acetone is commonly used to remove nail polish. This common substance was a dangerous
material that may be harmful if not properly kept and used. If it comes into contact with
the eyes or nose, it can cause severe irritation.
 Hydrogen peroxide is a mild antiseptic that aids in the cleaning of wounds and bruises as
well as the prevention of infection. Contrary to popular belief, hydrogen peroxide is a
dangerous chemical that can cause skin irritation, burning, and blistering. In addition,
swallowing can result in death.

3. Apply RAMP in assessing the situation presented in the video: https://www.youtube.com/watch?

The tragedy occurred when two graduate students tested and investigated the dynamic and
explosive chemical, according to the film titled Texas Tech Lab Explosion. Their chemical to be
tested is a novel compound that is a nickel hydrazine perchlorate derivative. They were given the
task of synthesizing and testing this. They first intended to scale their chemical in 300 mg
increments, but then modified this to 10 g. Without a prior knowledge and training with regards
to this new compound, they just keeping it wet with the solvent to remain it from explosion. As a
result, the senior graduate tries to pound the compound in a mortar and pestle in order to break up
the clumps. Due to the student's negligence, he left it for a few minutes before continuing to
pound the chemical mixture without wearing safety goggles or keeping a safe distance from the
hazardous substance.

The disaster might have been avoided if they had followed the safety principles, RAMP,
while analyzing the threats and risks inside the laboratory. They must first detect and identify the
risks posed by nickel hydrazine perchlorate. To ensure that the experiment runs well, they should
produce a material data sheet. In assessing the dangers associated with the exposures and
procedures to be included in their experiment, students should investigate what would happen if
they expose the chemical for an extended period of time outside. In order to minimize the risks,
they should constantly be aware of the personal safety equipment that they must wear within the
laboratory to protect themselves from threats. They should also work under chemical hoods to
avoid being exposed to the dangerous compounds they have been working with. Furthermore,
with this knowledge, one should prioritize and plan ahead of time in terms of emergency
preparation, such as, all laboratories must contain safety equipment and be acquainted in the
location of all protective equipment such as fire extinguisher, fire blankets, safety showers and
more, ensuring that you have the ability to operate every equipment and aid inside the laboratory.
All of the analysts/researchers' negligence inside the laboratory has resulted in significant injuries
and, if not addressed immediately, a tragic death. In conclusion, all laboratory personnel,
researchers, and analysts should prioritize "safety first" above all else. To avoid catastrophic
injuries and damage, we must constantly prioritize our own safety either in or out of the
laboratory.
 References:

 Gasoline fact sheet. (n.d.).

 Government of Canada, C. C. for O. H. and S. (2021, August 28). Acetone : OSH answers.
Canadian Centre for Occupational Health and Safety.
https://www.ccohs.ca/oshanswers/chemicals/chem_profiles/acetone.html.

 U.S. Department of Health and Human Services. (n.d.). Safe laboratory practices & procedures.
National Institutes of Health.
https://ors.od.nih.gov/sr/dohs/safety/laboratory/Pages/student_goodlab.aspx.

 Stanborough, R. J. (2019, November 13). 22 hydrogen PEROXIDE uses you can try today.
Healthline. https://www.healthline.com/health/hydrogen-peroxide-uses.

 RMSUofA. (2012, January 20). Texas tech Lab Explosion. YouTube.

 Hill, Robert H. Jr, and Finster, David C. (2016) Laboratory Safety for Chemistry Students, 2nd
ed. John Wiley & Sons, Inc.