SPECIES FACT SHEET

Scientific Name: Aeshna sitchensis (Hagen, 1861)

Common Name: Zigzag Darner
Phylum: Arthropoda
Class: Insecta
Order: Odonata
Suborder: Anisoptera
Family: Aeshnidae (darners)

Conservation Status:
Global Status (1985): G5 – Secure
National Status (United States): N3
National Status (Canada): N5
State Statuses: Alaska (SNR), Idaho (SNR), Maine (S4), Michigan (SNR),
Minnesota (SNR), Montana (S2S3), New Hampshire (SNR), Oregon
(SNR), Utah (SH), Washington (S2), Wisconsin (S1), Wyoming (SNR)
Province Statuses: Alberta (S3), British Columbia (S5), Labrador (SNR),
Manitoba (S4), New Brunswick (S2), Newfoundland Island (S4),
Northwest Territories (SNR), Nova Scotia (S2), Nunavut (SNR), Ontario
(S4), Quebec (S5), Saskatchewan (SNR), Yukon Territory (S5)
(NatureServe 2009).

Technical Description:
Adult: Aeshna sitchensis belongs to the family Aeshnidae, commonly
known as the darners. Dragonflies in this family are recognized by their
large bodies, large eyes, long abdomens with complex patterns of pale
spots, and thorax typically with two lateral stripes (Paulson 2009).
Relative to other darners, A. sitchensis is a small species with the lateral
thoracic stripes narrow and so strongly notched and extended that they
form a characteristic zigzag shape (Paulson 2009). The first (most
anterior) stripe, in particular, has a distinct zigzag pattern. The two
stripes are often broken and may have a spot or streak between them,
and the frontal stripes are reduced or lacking. As with all darners, the
large eyes touch each other at the top of the head. The face is generally
pale yellow with a black cross-line, and the dark wing spots are narrow
and elongate. Males of this species have brown eyes mixed with blue
around the edges, and all of the pale markings on the body are light blue
with no green or yellow. Females have two forms; the andromorph
(resembling the male) has blue eyes and blue pale markings on the body,
while the heteromorph (different than the male) has brown eyes with blue
highlights, yellow thoracic stripes, and yellow to white spots on the
abdomen (Paulson 2009).

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The small size (≤65 mm) and very blue abdomen of the male are obvious
and aid in field identification of this species (Paulson 2009). No other
Aeshna species whose ranges overlap with this species in Washington or
Oregon (e.g. A. juncea and A. umbrosa in Colville National Forest) are so
small and blue, nor do they have the characteristic zigzag lateral thoracic
stripes. In Alaska and Canada, this species could be confused with the
azure darner (A. septentrionalis), a similar small darner species which
also has zigzag lateral thoracic stripes but differs in the coloration of the
abdomen (see Paulson 2009). Aeshna septentrionalis does not occur in
Washington or Oregon.

Immature: Aeshnidae larvae (nymphs) are generally slender compared to

those of other families, with the extensible lower lip (labia) long and flat
(Wisconsin Odonate Survey 2010). Additionally, the larvae in this family
have 6- or 7-segmented antennae (as opposed to 4), 3-segmented middle
tarsi (as opposed to 2), and the ligula with a median cleft (Tennessen
2008). The segments of the antennae are usually slender and bristle-like
(as opposed to short, thick, and hairy) (Tennessen 2008). Aeshna larvae
are distinguished from other genera in the family as follows: lack of stout
raptorial setae on the dorsal surface of the palpal lobes of the labium;
rounded posterolateral margins of the head; antennae about half as long
as distance from their base to rear of head (as opposed to longer than
that distance); compound eyes much shorter (as opposed to longer) than
their greatest width; and the apex of the epiproct bifid or acute (as
opposed to truncate) (Tennessen 2008).

The nymphs of this species are small and rather slender, with the head
broadest across the middle of the eyes (Walker 1921). A description of
the nymph is as follows (Walker 1921): Body length 31.5 to 32.5 mm;
mentum of labium 4.5 to 5.0 mm; hind wing-pads 7.5 mm; hind femora
5.5 mm; inferior appendages 3.0 to 3.25 mm; ovipositor 2.25 to 2.5 mm;
width of head 7 mm; width of abdomen 6 mm. The head is nearly
uniform brownish in color, the thorax has traces of paler mottlings in
some specimens, and the abdomen is brownish with pale markings
almost exactly as in A. interrupta and A. eremita. A detailed description of
such markings is provided in Walker (1921). The head is similar to A.
juncea and A. interrupta, but the eyes are a shade more prominent than
A. interrupta. The lateral margins of the head are short, passing through
well-rounded angles into the posterior margin, which is straight or feebly
excavated when viewed directly from above. The mentum of the labium
reaches back to the middle of the mesacoxae, the basal breadth about
three-fifths of the apical breadth, and the greatest breadth four-fifths of
the length; the sides are nearly straight and feebly divergent from the
base to a point just beyond the middle, distad of which they are strongly
arcuate. The middle lobe is prominent, narrow, and obtusangulate,

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closely resembling that of A. juncea. The lateral lobes are broad, the
terminal parts subequal, the outer angle squarely truncate and scarcely
rounded, and the inner angle with a minute tooth. The supra-coxal
processes are rather short and blunt, subequal in length, with the
posterior one slightly stouter, and the interval nearly rectangular. The
abdomen is broadest at segments 6 and 7, a little more slender than in
A. interrupta. Lateral spines are present only on segments 7 to 9; those of
7 are minute rudiments, those of 8 extend half way to the base of
segment 9, and those of 9 extend as far as the proximal fourth of 10.
Further details regarding the inferior appendages and ovipositor are
provided in Walker (1921).

See Attachment 4 for illustrations of the nymph and photographs of the

adult of this species. Additional photographs can be viewed at
http://wiatri.net/inventory/odonata/SpeciesDetail.cfm?TaxaID=11 (Last
accessed 3 July 2010).

Life History:
The range-wide flight period for this species is from early June to October
(Wisconsin Odonate Survey 2010). In Washington, adults fly during mid
to late summer and adult specimens have been collected between July
21st and September 11th (Paulson 2010). Members of this family fly
continuously or hover intermittently, then hang vertically when they
perch (Paulson 2009). This species is an amazing aerialist with extremely
good sight, and is difficult to catch in flight. It is commonly approached
hanging on the bark of trees. It also often perches on the ground,
unusual for a darner (Bryan 2010). The males of this species fly low (only
a foot or two from the ground), sometimes apparently at random over the
bog and sometimes following the stream for some distance (Walker
1921). They fly less swiftly than others in this genus, and frequently drop
to the surface of the water for an instant, in a manner somewhat
suggestive of an ovipositing female (Walker 1921). The females have been
observed ovipositing in wet moss near the edges of small puddles in a
bog, many of which were less than a square foot in area. According to
Walker (1921), the manner of oviposition is similar to other Aeshna
species; the female alights on the moss and thrusts her abdomen into it
in various directions, apparently following no regular pattern. Usually
she remains in one spot for less than half a minute, then flies on a few
yards to repeat the operation. Occasionally, copulating pairs have been
seen rising from the bog and flying to neighboring trees (Walker 1921).

Larvae of this species feed on aquatic animals, including invertebrates

and small fish (Wisconsin Odonate Survey 2010). Adults feed on flying
insects. NatureServe (2009) designates sightings more than 3 kilometers
(1.9 miles) apart as separate populations, but little is known about their
dispersal and colonization ability. Like other darners, this species is a

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strong flier and may be a good colonist, able to reach sites several
kilometers apart. Upon emergence from the larval stage, young adults
(tenerals) may wander for a time before returning to their larval site or
another suitable area to mate. Some adults will usually be present at
locations where the species reproduces.

Range, Distribution, and Abundance:

Range-wide: The distribution of this species covers Canada and most of
the northern United States (NatureServe 2009, Paulson 2009). Based on
known records, Oregon, Wyoming, and Idaho form the southern border
in the west, and Minnesota, Michigan, and New Hampshire form the
southern border in the east (NatureServe 2009). The species was
historically present in Utah but is now thought to be extirpated from the
state (NatureServe 2009).

Washington: This species is known from Ferry, Okanogan, Pend Oreille,

Chelan and Skamania Counties, mostly at high elevations on Forest
Service land (Paulson 2010, Abbott 2010). The majority of observed and
collected specimens are from Bunchgrass Meadows, a Research Natural
Area (RNA) in Pend Oreille County.

Oregon: The distribution of this species in Oregon consists of a narrow

band covering the Willamette Valley and Cascade Range (Paulson 2009),
with records from Clackamas, Lane, and Deschutes Counties (Abbott
2010, Johnson and Valley 2005). According to Jim Johnson (pers. comm.
2010), there haven’t been any new localities found for this species in
Oregon since 2004, nor have there been any Oregon sightings since that
time, although the species is still thought to persist at a sedge meadow
near Sparks Lake, Deschutes County (the only Oregon site where it has
been found to be numerous). Little Crater Lake Meadow (Clackamas
County) dried out for a couple years and it’s possible that the conditions
left the area unsuitable for the species (Johnson 2010, pers. comm.).
Thus far, this species is unrecorded from the Blue Mountains (Johnson
and Valley 2005).

Forest Service/BLM lands: All known Washington and Oregon records for
this species are on Forest Service land (Paulson 2010, pers. comm.,
Johnson 2010, pers. comm.). In Washington, this species is documented
on Okanagon/Wenatchee National Forest (Fish Lake), Colville National
Forest (Davis Lake, Bunchgrass Meadows), and Gifford Pinchot National
Forest (Forlorn Lakes, South Prairie) (Paulson 2010, pers. comm.; Loggers
2010, pers. comm.; Kerst 2010, pers. comm.). In Oregon, this species is
documented on Mt. Hood National Forest (Little Crater Lake Meadow),
Willamette National Forest (Gold Lake Bog), and Deschutes National
Forest (Strider Lake and a sedge meadow near Sparks Lake)

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Abundance: Most collections in Washington and Oregon have been of one
or two individuals, but as many as 11 males and 4 females have been
collected at a single location/date, and greater than 50 individuals have
been observed at a single location/date (Bunchgrass Meadows, August
14th, 2007). According to Dennis Paulson (2010, pers. comm.), this
species is common and apparently well-established at most of the
Washington sites where it occurs. In Oregon, the species is only
numerous at one site (sedge meadow near Sparks Lake) (Johnson 2010,
pers. comm.).

Habitat Associations:
Wet sedge meadows, fens, bogs, and very shallow peaty ponds are the
reported habitat for this boreal species (Paulson 2009, 2010; Bryan
2010). According to the Wisconsin Odonate Survey website (2010), this
species prefers bog pools, ten square yards or less, usually without
emergent plants, including pools that dry in the summer. It can also be
found in shallow, evenly vegetated sedge/moss fens with puddles
(Wisconsin Odonate Survey 2010). Walker (1921) describes one breeding
site in British Columbia as a small mossy bog at the foot of a mountain,
fed by springs and seepage from a small, cold mountain brook. The bog
at this site was partly enclosed by spruce forest and there was practically
no aquatic vegetation other than the partly submerged moss (Walker
1921). The Washington sites range in elevation from 1850 ft. (Fish Lake,
Chelan County) to 3500 ft. (South Prairie, Skamania County) to 5000-
6000 ft. (northeast Washington) (Paulson 2010). This species co-occurs
with Somatochlora franklini, S. whitehousei, and other Somatochlora
species at Bunchgrass meadows and other sites (Walker 1921).

Threats:
Drought caused by global climate change is considered the primary
threat to this species (Paulson 2010, pers. comm.). Projected climate
changes in this region include increased frequency and severity of
seasonal flooding and droughts, reduced snowpack, increased siltation,
and increased air and water temperatures (Field et al. 2007), all of which
could impact this species’ habitat unfavorably. Moreover, since many
aspects of odonate survival (e.g. development, phenology, immune
function, pigmentation, and behavior) are sensitive to changes in
temperature, global climate change is predicted to have serious
consequences on this taxon (Hassall and Thompson 2008). As noted
above, Little Crater Lake Meadow recently dried out for a couple of years
and it is possible that the conditions left the area unsuitable for the
species (Johnson 2010, pers. comm.).

Habitat alteration and degradation may also threaten this species at

some sites. Specific activities that alter fen-habitat in Washington

5
include peat mining, wetland grazing, manipulation of water levels,
recreation, recreational development, and management of aquatic
vegetation (Fleckenstein 2006). Although the population at Bunchgrass
Meadows is now largely shielded from these threats (USDA Forest Service
2008), other potential populations in the area may not be. Insect and
disease control (still allowed in Bunchgrass Meadows) may threaten this
species. Livestock grazing is not permitted within the Bunchgrass
Meadows, although the LeClerc grazing allotment is adjacent to the RNA,
and could potentially impact the hydrology of the site. The loss of trees
through timber harvest poses additional threats to this species, since
trees provide (1) shade that maintains lower water temperatures for
larvae and (2) foraging and nighttime roosting areas for adults
(Packauskas 2005).

Stocking of non-native fish species for commercial or recreational

purposes could also negatively impact population survival, since the
dragonfly larvae may not be adapted to co-exist with such predators.

Conservation Considerations:
Bunchgrass Meadows, one of the few sites for this species in
Washington, is a unique and interesting site for Odonata. It is also the
only known Washington site for two Somatochlora species (S. franklini
and S. whitehousei), and home to a population of Coenagrion
interrogatum, a narrow-wing damselfly which is quite rare and local in
the state (Paulson 2008, pers. comm.). This site contains no non-native
vascular plant species and is noticeably unique in terms of both animal
and plant diversity (Ahlenslager 2008). It requires serious conservation
efforts, and has recently gained federal protection as an official Research
Natural Area (RNA) (USDA Forest Service 2008, Ahlenslager 2008, pers.
comm.). The main goal of an RNA is to provide opportunities for non-
manipulative and non-destructive research in ecosystems that are free
from human impact and influenced only by natural processes. Current
management of the Bunchgrass RNA includes prohibiting logging and
mining, discouraging recreational use (horseback riding and berry
picking are permitted), and reducing travel throughout the site.

Inventory: Survey for new sites near the known site at Bunchgrass
Meadows, and elsewhere in northeast Washington. The species may
occur in upland bog/fen habitat along the northern Washington border,
a region that has not yet been surveyed for odonates and is in critical
need of inventory (Paulson 2010, pers. comm.). The Oregon records are
some of the southernmost extensions of this species’ global distribution.
Since global climate change is expected to threaten southern
populations, continued surveys and abundance estimations at these
sites and surrounding areas would be valuable in evaluating distribution

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shifts, population declines, and other climate-driven effects. At a
minimum, the Sparks Lake site should be revisited; this site was last
visited in 2002 and is the only Oregon site where the species was found
to be numerous (Johnson 2010, pers. comm.). The site at Little Crater
Lake Meadow could also be revisited; this site has dried out in recent
years and whether or not it continues to host this species is of interest.

Management: Consider managing habitat-disturbing activities to

minimize impacts to sedge meadow, fen, and bog-habitat. The following
should be considered for management of bog/fen habitat (Sargent and
Carter 1999):

--Protect mineral-rich ground-water sources from pollution.

--Avoid destruction of existing hydrology (e.g. diverting, damming, or
altering water flow).
--Avoid disturbance of plant community (e.g. grazing or harvesting peat
or sphagnum).
--Avoid fertilizer use in or near wetland area, since such pollution can
drastically change plant communities, often in favor of invasive species.
--Create a buffer zone at least 91 m (100 yards) around the wetland (This
can be done by planting shrubs/grasses, or by keeping the area free of
disturbance, including roads and trails).

Prepared by: Sarah Foltz Jordan

Xerces Society for Invertebrate Conservation
Date: August 2010

Edited by: Sarina Jepsen

Xerces Society for Invertebrate Conservation
Date: August 2010

Final Edits: Rob Huff

FS/BLM Conservation Planning Coordinator
Date: February 8, 2011

ATTACHMENTS:
(1) References
(2) List of pertinent or knowledgeable contacts
(3) Maps of Oregon/Washington Distribution
(4) Photographs of Adult and Illustrations of Larva
(5) Odonata (Anisoptera) Survey Protocol, including specifics
for this species

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ATTACHMENT 1: References:

Abbott, J.C. 2007. “Aeshna sitchensis records.” OdonataCentral: An

online resource for the distribution and identification of Odonata. Texas
Natural Science Center, The University of Texas at Austin Available at:
http://www.odonatacentral.org (Accessed 2 July 2010).

Ahlenslager, K. 2008. Personal communication: E-mail exchange with

Sarah Foltz Jordan, Xerces Society, regarding the establishment of
Bunchgrass Meadows as a Research Natural Area.

Bryan, N. 2010. Zigzag Darner: Aeshna sitchensis. Talk About Wildlife.

Available at: http://talkaboutwildlife.ca/profile/?s=732 (Accessed 3 July
2010).

Field, C.B., Mortsch, L.D., Brklacich, M., Forbes, D.L., Kovacs, P., Patz,
J.A., Running, S.W. and Scott, M.J. 2007. Chapter 14: North America.
In: Climate Change 2007: Impacts, Adaptation and Vulnerability.
Contribution of Working Group II to the Fourth Assessment Report of the
Intergovernmental Panel on Climate Change (Parry, M.L., Canziani, O.F.,
Palutikof, J.P., van der Linden, P.J. and Hanson, C.E., eds.). Cambridge
University Press, Cambridge, UK. Available at:
www.ipcc.ch/pdf/assessment-report/ar4/wg2/ar4-wg2-chapter14.pdf
(Accessed 20 July 2010).

Fleckenstein, J. 2006. “Coenagrion interrogatum Species Fact Sheet.”

Interagency Special Status /Sensitive Species Program (ISSSSP). Jan.
2006. OR-WA Forest Service / Bureau of Land Management. Available at:
http://www.fs.fed.us/r6/sfpnw/issssp/species-index/fauna-
invertebrates.shtml (Accessed 2 August 2010) .

Hassall, C. and D.J. Thompson. 2008. The effects of environmental

warming on Odonata: a review. International Journal of Odonatology
11(2): 131-153.

Johnson, J. 2010. Personal communication: E-mail exchange with Sarah

Foltz Jordan, Xerces Society.

Johnson, J. and S. Valley. 2005. The Odonata of Oregon. Bulletin of

American Odonatology 8(4): 101-122.

Kerst, C. 2010. Personal communication: E-mail exchange with Sarah

Foltz Jordan, Xerces Society.

8
Loggers, C. 2010. Personal communication: E-mail exchange with Sarah
Foltz Jordan, Xerces Society.

NatureServe. 2008. “Aeshna sitchensis.” NatureServe Explorer: An online

encyclopedia of life [web application]. Feb. 2009. Version 7.1.
NatureServe, Arlington, Virginia. Available at:
http://www.natureserve.org/explorer (Accessed 2 August 2010).

Packauskas, R.J. “Hudsonian Emerald Dragonfly (Somatochlora

hudsonica): a technical conservation assessment.” 24 Aug. 2007. USDA
Forest Service, Rocky Mountain Region. 16 Oct. 2008. Available at:
http://www.fs.fed.us/r2/projects/scp/assessments/hudsonianemeraldd
ragonfly.pdf (Accessed 15 July 2010).

Paulson, D. 2008. Personal communication: E-mail exchange with Sarah

Foltz Jordan regarding Pacific Northwest odonates.

Paulson, D. 2009. Dragonflies and damselflies of the west. Princeton

University Press. 536 pp.

Paulson, D. 2010. Washington Odonata. Slater Museum of Natural

History, University of Puget Sound. Available at:
http://www.pugetsound.edu/academics/academic-resources/slater-
museum/biodiversity-resources/dragonflies/washington-odonata/
(Accessed 21 July 2010).

Paulson, D. 2010. Personal communication: E-mail exchange with Sarah

Foltz Jordan, Xerces Society.

Sargent, M.S and Carter, K.S., ed. 1999. Managing Michigan Wildlife: A
Landowners Guide.  Michigan United Conservation Clubs, East Lansing,
MI. 297pp.

Tennessen, K. 2008. Chapter 12. Odonata. In. Merritt R.W. and K.W.
Cummins. Ed. An Introduction to the Aquatic Insects of North America.
4th Edition. Kendall/Hunt Publishing Co. Dubuque, Iowa.

USDA Forest Service 2008. Environmental Assessment. Bunchgrass

Meadows Research Natural Area Establishment and Forest Plan
Amendment.
http://gis.fs.fed.us/r6/colville/projects/nepa/so/bunchgrass/20080708
-final-bunchgrass-rna-ea.pdf (Accessed 2 August 2010).

Walker, E.M. 1921. The nymph and breeding place of Aeshna sitchensis

Hagen (Odonata). Canadian Entomologist 53(10): 221-226.

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Wisconsin Odonate Survey. 2010. Most-wanted species: Aeshna
sitchensis. Available at:
http://wiatri.net/inventory/odonata/SpeciesDetail.cfm?TaxaID=11
(Accessed 3 July 2010).

ATTACHMENT 2: List of pertinent, knowledgeable contacts:

Chris Loggers
Dennis Paulson
Jim Johnson
Cary Kerst
Denis Doucet

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ATTACHMENT 3: Maps of Global Range/Conservation Status and
Oregon/Washington Distribution:

Records of Aeshna sitchensis in Washington and Oregon, relative to

USFS and BLM lands.

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ATTACHMENT 4: Illustrations of Nymph and Photographs of Adult:

Illustrations of the nymph of Aeshna sitchensis, extracted from Walker

(1921). 1: exuvia of full-grown male nymph; 2: head of full-grown male
nymph, direct dorsal view; 3: labium (closed), ventral view; 4: left supra-
coxal process; 5: caudal appendages of male, dorsal view; 6: terminal
abdominal segments of female nymph, ventral view.

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Aeshna sitchensis adult male, lateral view. Photograph by Denis Doucet,
used with permission.

13
Close-up photograph of Aeshna sitchensis adult male, showing dark
facial line and proportionately small eyes for a darner. Photograph by
Ben Coulter, used with permission.

ATTACHMENT 5: Odonata (Anisoptera) Survey Protocol, including

specifics for this species:

Survey Protocol

Taxonomic group:
Odonata

Where:
Adult odonates can be found feeding in range of terrestrial habitats, but
are most effectively sampled at the aquatic habitat where they mate and
oviposit. Ponds, streams, rivers, lake shores, marshes, bogs, and fens
support a range of odonate diversity. Some species (e.g. Gomphus kurilis)
frequent a variety of habitats, while others (e.g. Leucorrhinia borealis)

14
have highly specific preferences with regard to substrate, vegetation, and
water quality. For species-specific habitat information, see the section at
the end of this protocol.

When:
Adults are surveyed in summer, during the often-short window of their
documented flight period. Adult odonates are most active in warm
temperatures, and usually begin to fly at the aquatic habitat with the
morning sun. Depending on the species, males arrive as early as 9 am
and leave as late as 6 pm. Females tend to arrive several hours later,
after the males have established their mating territories (Campanella
1975). In the high temperatures of the late afternoon, some species seek
shade in trees and vegetation.

Although larvae are present all summer, it is preferable to sample later in

the season (i.e. just prior to and during the early part of adult
emergence), when a higher proportion of the more easily identified late
larval instars will be found.

Adult Surveys:
Use a long-handled, open-mesh aerial net, light enough to be swung
rapidly. Triplehorn and Johnson (2005) recommend a 300-380 mm
diameter net with a handle at least 1 m long.

Approach the site quietly, observing the environment and natural

behaviors occurring prior to sampling. Note the number of different
species present, and what their flight patterns are. This will help in
predicting the movement of target species, and in evaluating whether the
site has been surveyed “exhaustively” (i.e. all species observed at the site
have been collected or photodocumented). Since dragonflies are wary of
humans and readily leave an area when disturbed, it is important to be
as discreet in your movements as possible, at all times.

Watch vegetation, logs, tree-trunks, and large, flat rocks for perched
individuals, particularly those in the Gomphidae and Libellulidae
families. Since dragonflies are powerful fliers and notoriously challenging
to catch, try to quietly photo-document specimens prior to attempting to
capture. Use a camera with good zoom or macrolens, and focus on the
aspects of the body that are the most critical to species determination
(i.e. dorsum of abdomen, abdominal terminalia (genitalia), pleural
thoracic markings, wing markings, eyes and face). For helpful tips, see
the article “Photographing Dragonflies” (Nikula 1997) available at:
http://www.odenews.org/PhotoArticle.htm (last accessed: 25 Oct. 2008).

15
When stalking perched individuals, approach slowly from behind,
covering your legs and feet with vegetation, if possible (dragonflies see
movement below them better than movement at their level). When
chasing, swing from behind, and be prepared to pursue the insect. A
good method is to stand to the side of a dragonfly’s flight path, and swing
out as it passes. After capture, quickly flip the top of the net bag over to
close the mouth and prevent the insect from escaping. Once netted, most
insects tend to fly upward, so hold the mouth of the net downward and
reach in from below when retrieving the specimen. Collected specimens
should be placed on ice in a cooler long enough to slow their movement
(a few minutes), and then set on a log or stone and comprehensively
photographed until the subject starts to stir. Specimens to be preserved
should be placed alive, wings folded together, in glassine or paper
envelopes, as they lose color rapidly once killed. Record the eye color and
locality/collection data on the envelope, including longitude and latitude
if possible.

Acetone, which helps retain bright colors, is recommended for killing

odonates. Glassine envelopes with the lower corner clipped and the
specimen inside should be soaked in acetone for 24 hours (2 to 4 hours
for damselflies) and then removed, drained, and air-dried. The resulting
specimens are extremely brittle, and can be stored in envelopes, pinned
with wings spread, or pinned sideways to conserve space. Mating pairs in
tandem or copula should be indicated and stored together, if possible.
Collection labels should include the following information: date, time of
day, collector, detailed locality (including water-body, geographical
coordinates, mileage from named location, elevation, etc.), and detailed
habitat/behavior (e.g. “perched on log near sandy lake shore”). Complete
determination labels include the species name, sex (if known),
determiner name, and date determined.

Relative abundance surveys can be achieved by timed watches at

designated stations around a site. We recommend between 5 and 10
stations per site, each covering one square meter of habitat, and each
monitored for 10 to 15 minutes. Stations should be selected in areas
with the highest odonate usage, and spread out as evenly as possible
throughout the site. During and one minute prior to the monitoring
period, observers should remain very still, moving only their eyes and
writing hand. Recorded information should include start and end times,
weather, species, sex, and behavior (e.g. male-male interaction, pair in
tandem). Observations occurring near, but outside of, the designated
station should be included but noted as such.

Catch and marked-release methods can help evaluate population sizes,

species life-span, and migration between sites. This strategy (most

16
appropriate if several sites are being surveyed repeatedly throughout a
season) involves gently numbering the wing with a fine-tip permanent
marker before release.

Larval Surveys:
When surveying for larvae, wear waders, and use care to avoid disrupting
the stream banks, vegetation, and habitat. Depending on the habitat, a
variety of nets can be useful. D-frame nets are the most versatile, as they
can be used in both lotic and lentic habitats. Kick-nets are only useful
when sampling stream riffles, and small aquarium nets are most effective
in small pools. If desired, relative abundance between sites or years can
be estimated by standardizing sampling area or sampling time. When the
use of a D-frame net is not feasible (e.g. in areas that have very dense
vegetation, little standing water, and/or deep sediment), an alternative
sampling device, such as a stovepipe sampler, can be used. This
cylindrical enclosure trap (~34 cm in diameter and 60 cm in height) is
quickly forced down through the water/vegetation and firmly positioned
in the bottom substrate. Material and organisms are then removed by
hand using small dip nets (Turner and Trexler 1997).

Net contents are usually dumped or rinsed into shallow white trays to
search for larvae more easily, as they are quite cryptic and can be
difficult to see if they are not moving. White ice-cube trays may also aid
in field sorting. Voucher collection should be limited to late instar larvae,
which can be most readily identified. If necessary, early instars can be
reared to later stages or adulthood in screened buckets/aquaria with tall
grasses added for emergence material. However, since the rearing
process often takes many trials to perfect, it is only recommended if
knowledge of species’ presence-absence status at a particular site is
critical, and few-to-no late instars or adults are found.

Voucher specimens can be either (1) preserved on-site in sample vials

filled with 80% ethanol, or (2) brought back from the field in wet
moss/paper-towels, killed in boiling water, cooled to room temperature,
and transferred to 80% ethanol. Although the latter method is more time
intensive, it is recommended for maximum preservation of internal
anatomy (Triplehorn and Johnson 2005). Live specimens should be
separated by size during sorting to reduce cannibalism/predation.

Although easily overlooked, larval exuviae left on rocks, sticks, or

vegetation on which the adult emerged are valuable for species
documentation. These cast-off exoskeletons of the final larval instar can
be identified to species using larval traits, and offer a unique,
conservation-sensitive sampling method for odonates (Foster and Soluk
2004). Since exuviae indicate the presence of successful breeding

17
populations at a particular locale, their habitat data can be very
informative, and should be documented with as much care as that of
larvae and adults.

Species-specific survey details:

Aeshna sitchensis
Since this species appears to be common and stable at most known
Washington sites, surveys in Washington should focus on uncovering
new sites in unsurveyed regions in the northeastern part of the state
(Paulson 2010, pers. comm.). The species may occur in upland bog/fen
habitat near the existing site at Bunchgrass Meadows, and along the
northern Washington border, a region that has not yet been surveyed for
odonates and is in critical need of inventory (Paulson 2010, pers. comm.).

The Oregon records are some of the southernmost extensions of this

species’ global distribution. Since global climate change is expected to
threaten southern populations, continued surveys and abundance
estimations at these sites and surrounding areas would be valuable in
evaluating distribution shifts, population declines, and other climate-
driven effects. At a minimum, the Sparks Lake site should be revisited;
this site was last visited in 2002 and is the only Oregon site where the
species was found to be numerous (Johnson 2010, pers. comm.). The site
at Little Crater Lake Meadow should also be revisited; this site has dried
out in recent years and whether or not it continues to host this species is
of interest.

Bogs and wet meadows (fens) are the appropriate habitat in which to
conduct surveys for this species. Sites should be surveyed midday,
between late July and early September, and approached quietly in search
of perched adults. Members of this family fly continuously or hover
intermittently, then hang vertically when they perch (Paulson 2009). This
species is an amazing aerialist with extremely good sight, and is difficult
to catch in flight. It is commonly approached hanging on the bark of
trees. It also often perches on the ground, unusual for a darner (Bryan
2010). The females may be collected in wet moss near the edges of small
bog puddles, where oviposition occurs (Walker 1921). The males of this
species fly low (only a foot or two from the ground), sometimes
apparently at random over the bog and sometimes following the stream
for some distance (Walker 1921). They fly less swiftly than others in this
genus, and frequently drop to the surface of the water for an instant, in a
manner somewhat suggestive of an ovipositing female (Walker 1921). The
small size helps distinguish this species from other darners, although in-
hand examination is usually necessary to see the characteristic zigzag
pattern on the thorax.

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While researchers are visiting sites and collecting adults and exuviae,
detailed habitat data should also be acquired, including substrate type,
water quality, vegetation characteristics, and presence/use of canopy
cover (Packauskas 2007). Abundance estimates for this species would
also assist future conservation efforts, since population size is important
in evaluating the stability of a species at a given locality.

References (Survey Protocol Only):

Bryan, N. 2010. Zigzag Darner: Aeshna sitchensis. Talk About Wildlife.

Available at: http://talkaboutwildlife.ca/profile/?s=732 (Accessed 3 July
2010).

Campanella, P.J. 1975. The evolution of mating systems in temperate

zone dragonflies (Odonata: Anisoptera) II: Libellula luctuosa (Burmeister).
Behaviour 54: 278-310.

Foster, S.E. and D.S. Soluk. 2004. Evaluating exuvia collection as a

management tool for the federally endangered Hine's emerald dragonfly,
Somatochlora hineana Willaimson (Odonata: Cordulidae). Biological
Conservation 118: 15-20.

Johnson, J. 2010. Personal communication with S. Foltz Jordan, Xerces

Society.

Merritt, R.W., Cummins, K.W., and M.B. Berg. 2008. An introduction to

the aquatic insects of North America. Fourth Edition. Kendall/Hunt
Publishing Co., Dubuque, Iowa. 1158pp.

Nikula, B. 1997. “Photographing Dragonflies” 13 September 1997 Ode

News. 22 Oct. 2006 <http://www.odenews.org/PhotoArticle.htm>.

Packauskas, R.J. “Hudsonian Emerald Dragonfly (Somatochlora

hudsonica): a technical conservation assessment.” 24 Aug. 2007. USDA
Forest Service, Rocky Mountain Region. 16 Oct. 2008
<http://www.fs.fed.us/r2/projects/scp/assessments/hudsonianemerald
dragonfly.pdf >

Paulson, D. 2008. Personal communication: Email exchange with S.

Foltz Jordan, Xerces Society.

Paulson, D. 2009. Dragonflies and damselflies of the west. Princeton

University Press. 536 pp.

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Paulson, D. 2010. Personal communication: Email exchange with S.
Foltz Jordan, Xerces Society.

Triplehorn, C. and N. Johnson. 2005. Introduction to the Study of

Insects. Thomson Brooks/Cole, Belmont, CA. 864pp.

Turner, A.M. and J.C. Trexler. 1997. Sampling Aquatic Invertebrates

from Marshes: Evaluating the Options. Journal of the North American
Benthological Society 16(3): 694-709.

Walker, E.M. 1921. The nymph and breeding place of Aeshna sitchensis

Hagen (Odonata). Canadian Entomologist 53(10): 221-226.

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