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    DRC of Acetylcholine

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    Bhargav Deka
    The experiment aims to study the dose response curve of acetylcholine on frog rectus abdominal muscles. Various apparatus are used, including an organ bath and recording drum. The frog rectus muscle is isolated and mounted in the bath containing physiological salt solution. Graded doses of acetylcholine chloride from 1 to 100 μg/mL are added to the bath at intervals and the contractile responses are recorded. The observations are tabulated showing that higher doses of acetylcholine result in greater muscle contraction responses, with the maximum response at 100 μg/mL.

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    DRC of Acetylcholine

    Uploaded by

    Bhargav Deka
    74 views3 pages
    The experiment aims to study the dose response curve of acetylcholine on frog rectus abdominal muscles. Various apparatus are used, including an organ bath and recording drum. The frog rectus muscle is isolated and mounted in the bath containing physiological salt solution. Graded doses of acetylcholine chloride from 1 to 100 μg/mL are added to the bath at intervals and the contractile responses are recorded. The observations are tabulated showing that higher doses of acetylcholine result in greater muscle contraction responses, with the maximum response at 100 μg/mL.

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    DRC of Acetylecholine

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    The experiment aims to study the dose response curve of acetylcholine on frog rectus abdominal muscles. Various apparatus are used, including an organ bath and recording drum. The frog rectus muscle is isolated and mounted in the bath containing physiological salt solution. Graded doses of acetylcholine chloride from 1 to 100 μg/mL are added to the bath at intervals and the contractile responses are recorded. The observations are tabulated showing that higher doses of acetylcholine result in greater muscle contraction responses, with the maximum response at 100 μg/mL.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    74 views3 pages

    DRC of Acetylcholine

    Uploaded by

    Bhargav Deka
    The experiment aims to study the dose response curve of acetylcholine on frog rectus abdominal muscles. Various apparatus are used, including an organ bath and recording drum. The frog rectus muscle is isolated and mounted in the bath containing physiological salt solution. Graded doses of acetylcholine chloride from 1 to 100 μg/mL are added to the bath at intervals and the contractile responses are recorded. The observations are tabulated showing that higher doses of acetylcholine result in greater muscle contraction responses, with the maximum response at 100 μg/mL.

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    Experiment No:

    AIM OF THE EXPERIMENT: TO STUDY THE DOSE RESPONSE CURVE (DRC) OF


    ACETYLCHOLINE ON RECTUS ABDOMINAL MUSCLES OF FROG.
    APPARATUS:
    Reservoir, tubing, haemostatic forceps, isolated organ bath, aeration tube, isotonic frontal
    writing lever and recording drum.
    EXPERIMENTAL CONDITION:
    Physiological Salt solution (PSS): Frog’s ringer Temperature: 37 (+ or -) 10C
    Aeration: Carbogen (95% O2 and 5% CO2)
    Basal tension on the tissue: 1 gm Magnification of the response : 10 times
    Drug: Acetylcholine Chloride (1, 10 or 100 μg/mL)
    Molecular weight of drug: 181.78
    THEORY:
    Graded Dose Response Relationship Curve of Acetylcholine on Frog Rectus Muscle:
    In graded dose response curve,
    ➢ Single biological unit, either a single animal or an isolated tissue is used.
    ➢ It depends upon an observation that graded increase (in geometric proportion) in the
    dose of drug gives proportional rise in the magnitude of biological response.
    ➢ Actually, beyond a specific dose level, biological response increases in proportion to
    the increase in dose. This dose level is known as ‘Threshold dose’.
    ➢ Such proportional rise in biological response occurs only up to a dose level known as
    ‘Ceiling dose’, beyond which a steady biological response is achieved even after
    increasing the doses.
    ➢ Shape of Graded DRC, when plotted as ‘dose Vs Response’ is a ‘Parabola’
    ➢ Shape of ‘Log Dose Vs Response’ curve is a ‘Sigmoid’ line or is having ‘S’ like shape.
    PROCEDURE:
    The assembly is set up and the arrangements are made for the above-mentioned condition.
    ➢ A frog is sacrificed as per CPCSEA recommended guidelines.
    ➢ The frog is placed in a tray with ventral side facing up. The skin is incised longitudinally
    in the middle of abdomen from pubic symphysis to the sternum. Two recti are situated
    on the either side of the midline. They are dissected out by cutting its attachment from
    pubic symphysis from below, sternum from above and abdominal muscles from sides.
    The recti can be easily differentiated from other muscles because the recti are white and
    shiny whereas other muscles are pinkish in colour.
    ➢ Two recti are separated from the midline and one recus muscle is mounted in the organ
    bath. One end of the muscle is tied to the aeration tube and the other is connected to the
    isolated frontal writing lever.
    ➢ The tissue is allowed to stabilize for half an hour. During this period the PSS is changed
    after every ten min. once the tissue is stabilize, graded doses of Ach are added to at
    defined time period of interval for obtain contractile responses.
    • 00 sec: Start the drum and record a base line for 30 sec.
    • 30 sec: Add the first dose of drug in organ bath and take the response for another 30sec.
    • 60: Stop the drum and give wash until the tip of lever rich to baseline.
    • Continue above procedure for next doses.
    ➢ Measure the height of concentration at different doses of Ach.
    ➢ Tabulate the observations into three columns as Dose of Ach, Height of concentration
    (in mm) and % response.

    GRAPH:
    OBSERVATION TABLE:

    Sl Drug Conc. of Dose of drug in Response in mm %


    No Name drug mL Response
    1. 0.1 2 20
    2. 1 0.2 5 25
    3. µg/mL 0.4 7 35
    4. 0.8 9 45
    5. 0.16 12 60
    Ach 10
    6. 0.32 14 70
    µg/mL
    7. 0.64 16 80
    8. 0.128 17 85
    100
    9. 0.256 20 100
    µg/mL
    10. 0.512 12 60

    Maximum Response

    Report: Maximum response obtained at 100 µg/mL.

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