S P E C I F I C AT I O N S H E E T

Element AVITI™ System

Performance
Cost
Flexibility

The Element AVITI System offers an unrivaled combination of

performance, cost, and flexibility in a benchtop sequencer.

Based on proprietary Avidity

Sequencing™ chemistry

For Research Use Only.

Not for use in diagnostic
procedures.
Element AVITI System
The Element AVITI System reinvents surface chemistry, base detection, and data analysis to offer a flexible and cost-effective
platform for a variety of next-generation sequencing (NGS) applications. Overarching compatibility with standard NGS libraries provides
a straightforward access point to in-house sequencing while integrated and user-friendly software tools streamline operations.

Powerful Sequencing Unrivaled Cost and Flexibility

The AVITI System combines the performance, A wide array of sequencing library inputs can be made compatible for
flexibility, and affordability of a benchtop system while sequencing on the AVITI System through easy kitted solutions. The intuitive
producing superior data quality and per gigabase operating software includes a unique run setup feature that allows tunable
(Gb) costs comparable to a larger factory-scale read throughput for shorter run times. When combined with the onboard
sequencer. Random access dual flow cell capability tunable read throughput feature at run setup, the highly efficient, single
enables independent sequencing start times and flow cell reagent design makes ordering and inventory management simple
scalable output up to 480 Gb combined. and economical across a range of cycles and read throughputs.

AVITI System Performance Summary

The AVITI System delivers high-performance sequencing metrics through its simple single flow cell and reagent cartridge design.

Table 1: AVITI System performance parameters at full read depth with 800 MILLION paired-end reads per flow cell (full scan)

Single Flow Cell Dual Flow Cell

Read Length* Run Time Data Quality
Output (Gb) Output (Gb)

2x150 240 480 48hrs

2x100 160 320 35hrs

2x75 120 240 29hrs %Q30 > 90

2x50 80 160 23hrs

2x25 40 80 17hrs

Output specifications based on a single flow cell using Element PhiX Control library at supported polony densities.
* Paired-end reads per flow cell. Two flow cells can run in parallel or each can be started independently.

AVITI Tunable Throughput for Maximum Control

Read throughput is controlled through the AVITI Operating Software during run setup. This feature gives the user maximum control
over the desired combination of turnaround time, read length, and read depth without the burden of maintaining a complicated
inventory of different flow cells and reagents.

Table 2: Examples of read throughput times using common paired-end read lengths

Read Throughput* 2x25 2x50 2x75 2x100 2x150

800M (full scan) 17hrs 23hrs 29hrs 35hrs 48hrs

600M (3/4 scan) 16hrs 21hrs 27hrs 32hrs 43hrs

400M (1/2 scan) 15hrs 20hrs 24hrs 29hrs 39hrs

200M (1/4 scan) 14hrs 18hrs 22hrs 26hrs 34hrs

100M (1/8 scan) 13hrs 17hrs 21hrs 24hrs 31hrs

* Paired-end reads per flow cell. Two flow cells can run in parallel or each can be started independently.

Element AVITI™ System | Specification Sheet 2

 A

B C
D

F
H
G

Instrument Layout Parts

The random access dual flow cell design is like having two A Touchscreen user interface for run setup
sequencing instruments on your benchtop for the cost of B Fluidics pump bay side A
one. Each side is independently controlled through the AVITI C Fluidics pump bay side B
Operating Software. The intuitive touchscreen interface is D Flow cell nest bay
positioned in the middle of the unit for convenient proximity to E Ventilated housing
all its functional bays. The instrument also features electronic F Two waste bottles, one per flow cell
sensors for bar code identification of reagents for workflow G Sequencing reagent cartridge side A
guidance and error avoidance. Door lock sensors provide safety H Sequencing reagent cartridge side B
during operation.

Instrument Status Lights Flow Cell Nest Bay Status Indicators

A prominent indicator light on each side of the instrument A button unlatches and opens the lid to a 40° angle. A wall
communicates the instrument status so it is visible from the front encircles the loading area to guide placement and an LED light
facing view at either side. in front of each nest indicates status.

Color Status Color Status

Blue fade Sequencing of washing Blue The flow cell can be unloaded

Green fade Run or wash complete Green The flow cell is loaded and ready

Red fade Error Red The flow cell is improperly loaded

White and geen fade Idle Off The flow cell cannot be unloaded

Yellow fade Warning

Element AVITI™ System | Specification Sheet 3

Element NGS Workflow
Figure 1: The Element NGS workflow is designed to help scientists use all the genomics resources they have at their disposal

1. Library Prep 2. Avidity Sequencing™ 3. Data Analysis

For existing & de novo libraries On the Element AVITI system Designed so you connect easily
• Seamless compatibility with leading • Novel chemistry yields exceptional • Intuitive AVITI Operating Software
assays data quality • FASTQ file output for open access
• 96 unique index pairs for multiplexing • Combined efficiencies greatly reduce • ElemLAB™ fleet connectivity software
run costs
• Easy protocols with little hands-on time
• Two random access flow cells for
flexible run starts

Avidite Chemistry Is Key to the AVITI System

Avidity Sequencing reimagines the critical components of NGS and maximizes the combined impact on the accuracy and efficiency
of the cycled reaction. At its core, the AVITI System is built on the unique properties of its avidite chemistry, which has binding
efficiencies that yield high accuracy and reduce reagent consumption by more than log order. This innovation allows the system to
produce state-of-the-art sequencing quality at unmatched sequencing costs in a benchtop format.

A Wide Selection of Sequencing Library Inputs

The AVITI System surface chemistry is designed with a unique proprietary attachment chemistry and oligonucleotide sequences to
achieve optimum sequencing accuracy and cycling efficiency. To adapt to this surface chemistry, the AVITI System comes with both
standard and de novo NGS library prep options to fit seamlessly into nearly any genomics applications workflow.

Element Adept™ Library Compatibility Element Elevate™ Library Prep Workflow

Workflow The Elevate Workflow is designed as a substitution of adapters
in a standard NGS library preparation kit chosen by the user. The
The Adept Workflow is specifically designed to circularize
Elevate Workflow is based on two kits, the Element Elevate Index
standard NGS linear libraries, including PCR-free workflows, to
and Adapter Kit and the Element Elevate Library Circularization
make them compatible for sequencing on the AVITI System.
Kit. The index plate contains 96 unique index pairs for
Standard libraries also include those originated from specialized
multiplexing strategies. As with the Adept Library Compatibility
technologies such as Gel Bead-in-Emulsion from 10X Genomics.
Kit, the Elevate Library Circularization Kit contains 24 reactions
Libraries can be pooled or circularized individually in a single
and qPCR reagents for quantitation. Indexed libraries can be
reaction. The Element Adept Library Compatibility Kit is fully
pooled first or circularized individually before pooling and
kitted with 24 reactions and includes a quantitative PCR (qPCR)
sequencing on the AVITI System.
standard and primer mix to aid in quantitation and accurate
instrument loading.

Open Access Data Analysis Onboard Telemetry

The AVITI Operating Software converts base calls into FASTQ The AVITI System is factory equipped with onboard system
files that are not dependent on any proprietary database or sensors to measure performance metrics of key components
secondary analysis software. within the system to enable service and support. Raw sequence
data generated from the AVITI System is never accessed
through telemetry.

Element AVITI™ System | Specification Sheet 4

Element AVITI System for NGS
Meticulous specifications for performance and quality ensure accurate and standardized results.

Table 2: AVITI System specifications

Specifications

Performance Parameters Flow cells: Two random access

Read pairs passing filter per flow cell: ≥ 800 million
Maximum Read Length: 2 x 150 bp

Operating Environment Temperature: 18–26°C

Altitude: < 2000 m
Sound level: < 62 db at 3.3 feet

Physical Dimensions (H × W × D) 29.5 in × 36.7 in × 29.5 in

Weight: 155.1 kg / 342 lbs

Crated Dimensions (H × W × D) 48.6 in × 51 in × 35 in

Weight: 245.9 kg / 527 lbs

Power Requirements 100–240 VAC @ 50/60 Hz, 15 A, 550 W (average)

Operating System (OS) Ubuntu 20.04 LTS

Safety and Compliance NRTL certified IFC

FCC/IC approved

Power Cord and Ethernet Cable Protective Earth and Fuses

In the US, the AVITI System ships with the power cord NEMA An Element supplied grounded cable connects the instrument
5-20P to IEC 320-C19. Each instrument requires one delegate to protective earth.
outlet with single-phase 20 amp. Plug the power cord directly
into a standard wall outlet (NEMA 5-20R). Do not use an The instrument does not contain any user-replaceable fuses.
extension cord. The instrument also ships with an Ethernet cable Any fuse that needs to be replaced will require an Element
that you can plug into any Ethernet port. The power cord and specialist to install.
Ethernet cable are each 3 m (10 ft) long.

Preparing your Site for an AVITI System

Performing the Element AVITI System Workflow requires preparing lab space to accommodate the Element AVITI System. The AVITI
System is a benchtop instrument that integrates dedicated chemistry, hardware, and software to amplify and sequence DNA libraries.
Specific preparation will be necessary to prepare for its operation in the laboratory. The following additional items and physical
requirements are all listed in the Element AVITI System Site Prep Guide (MA-00007) at go.elembio.link/user-guides. These include,
without limitation, the following requirements:

• Stable benchtop or table with adequate spacing for ventilation and access
• Power requirements related to plug receptacle and circuit load

Element AVITI™ System | Specification Sheet 5

Ordering Information Installation
Product Catalog # When purchasing a new AVITI System, the instrument comes
complete with accessories, software, and instructions for use.
Element AVITI™ System 880-00001 The instrument includes a standard one-year, full parts and labor
warranty. The instrument is fully installed by an Element field
AVITI Sequencing Kit 860-00001 service engineer and includes two AVITI Sequencing Kits, an
Adept Library Compatibility Kit, and a PhiX Control Library for
Element Adept™ Library 830-00003 spike-in, so you can immediately begin using the AVITI System.
Compatibility Kit

Element Elevate™ Index and

Adapter Kit
830-00005 Learn More
To learn more about the AVITI Systems,
Element Elevate™ Library 830-00001 visit: go.elembio.link/aviti
Circularization Kit

PhiX Control Library, Adept 830-00004

PhiX Control Library, Elevate 830-00002

Element Biosciences
elementbiosciences.com
Telephone: 619.353.0300
Email: info@elembio.com

Document # LT-00002
For Research Use Only. Not for use in diagnostic procedures.

Information in this document is provided for research use only and is subject to change without notice.

© 2022 Element Biosciences, Inc. All rights reserved. Element Biosciences, Adept, Aviti and the Element Biosciences logo are trademarks of Element Biosciences, Inc. Other
names mentioned herein may be trademarks of their respective companies.
Visit elementbiosciences.com for more information.

Element AVITI™ System | Specification Sheet 6