Scribd Logo
Upload

Welcome to Scribd!

  • 2K views

    BOOM Extraction Principle

    Uploaded by

    Carlos Gooseman Casanova
    bioMérieux's proprietary BOOM technology uses magnetic silica particles to extract nucleic acids from clinical specimens. The extraction process involves lysing samples with guanidine thiocyanate to release nucleic acids, which then bind to the silica particles. Following washing cycles, nucleic acids are eluted and purified for downstream applications such as PCR. Several companies have licensed the BOOM technology for use in their own nucleic acid extraction kits.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd

    BOOM Extraction Principle

    Uploaded by

    Carlos Gooseman Casanova
    2K views11 pages
    bioMérieux's proprietary BOOM technology uses magnetic silica particles to extract nucleic acids from clinical specimens. The extraction process involves lysing samples with guanidine thiocyanate to release nucleic acids, which then bind to the silica particles. Following washing cycles, nucleic acids are eluted and purified for downstream applications such as PCR. Several companies have licensed the BOOM technology for use in their own nucleic acid extraction kits.

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    bioMérieux's proprietary BOOM technology uses magnetic silica particles to extract nucleic acids from clinical specimens. The extraction process involves lysing samples with guanidine thiocyanate to release nucleic acids, which then bind to the silica particles. Following washing cycles, nucleic acids are eluted and purified for downstream applications such as PCR. Several companies have licensed the BOOM technology for use in their own nucleic acid extraction kits.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    2K views11 pages

    BOOM Extraction Principle

    Uploaded by

    Carlos Gooseman Casanova
    bioMérieux's proprietary BOOM technology uses magnetic silica particles to extract nucleic acids from clinical specimens. The extraction process involves lysing samples with guanidine thiocyanate to release nucleic acids, which then bind to the silica particles. Following washing cycles, nucleic acids are eluted and purified for downstream applications such as PCR. Several companies have licensed the BOOM technology for use in their own nucleic acid extraction kits.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 11

    BOOM

    bioMérieux’s proprietary technology

    Extraction Principle

    Marina Perrotte, 08/2007


    Nucleic acid extraction
    procedure
    Various methods have been described for the release and/or extraction of
    nucleic acids from a wide variety of clinical specimens.
    The purpose of these methods is to:

     disrupt infected cells and microorganisms, resulting in the release of nucleic acids
     protect the nucleic acids from degradation
     remove inhibitors of amplification
     concentrate the target nucleic acid
     recover nucleic acid in an environment suitable for use in polymerase chain reaction (PCR)

    The complexity of the method chosen will depend on several factors. These include:
     type and volume of specimen to be tested
     concentration of target nucleic acid present/volume of specimen
     nucleic acid sought (DNA and/or RNA; single stranded vs double stranded)
     presence of inhibitors of the PCR which may interact with the target nucleic acid and/or
    the polymerase enzyme
     facilities available
     safety requirements

    Frank SIMONS | Slide 2


    Marina Perrotte, 08/2007
    Supports

    Different commercial supports exist for Nucleic Acids extraction


    Commercial kits can be specialized for DNA and/or RNA extraction

     Liquid reagents
     For high specimen input volume
     Centrifugation
     Automated procedure
     Columns with Membrane or Silica
     Format individual or 96-well microtiter plate
     Aspiration or centrifugation
     In 96-well microtiter plate, can be automated
     Silica particles (non Magnetic)
     Centrifugation
     Manual
     Magnetic Silica particles
     Magnet use
     Automated procedure
    Frank SIMONS | Slide 3
    Marina Perrotte, 08/2007
    BOOM
    Extraction Method

     Samples lysed in lysis buffer containing chaotropic agent: guanidine thiocyanate (GuSCN).
    Cells, bacteria, and viruses present in the samples are lysed and proteins such as nucleases
    are denatured and inactivated
     Nucleic acids (RNA and DNA) are released and bind to silica particles
     Following several washing cycles using wash buffers.
     In the final step, the nucleic acids are eluted from the silica particles in an elution buffer
     Eluate is highly purified and concentrated nucleic acid for subsequent amplification,
    sequencing and other molecular diagnostic analyses.
    Frank SIMONS | Slide 4
    Marina Perrotte, 08/2007
    BOOM
    Licences
    Different companies have a licence for BOOM
    technology use and to be allowed to sell their
    Nucleic Acids extraction methods

    N
    IO
    D T
    A R AC
    M ND XTR
    O TA E
    O
    B S DS Most of them are competitors for bioMérieux:
    LD CI Roche
    O
    G IC A Bayer
    H E E Qiagen
    T C L
    U Promega
    N Toyobo
    R
    FO Takara
    Eppendorf
    Diagnocure
    Visible Genetics
    Frank SIMONS | Slide 5
    Marina Perrotte, 08/2007
    BOOM Next generation
    Magnetic silica vs.
    non-magnetic silica

    1 µm
    1 µm

    magnet
    magnetic silica non magnetic silica

    silica • better surface / weight ratio: higher binding capacity


    • easier to manipulate and automate
    Frank SIMONS | Slide 6
    Marina Perrotte, 08/2007
    Magnetic silica
    Efficient mixing

    magnet

    Marina Perrotte, 08/2007


    silica Frank SIMONS | Slide 7
    Magnetic silica
    Efficient mixing

    magnet

    easyMAG
    disposable

    Frank SIMONS | Slide 8


    Marina Perrotte, 08/2007
    Magnetic Silica particles
    Advantages

    Frank SIMONS | Slide 9


    Marina Perrotte, 08/2007
    Magnetic Silica particles
    Advantages
    High Purity & Recovery
     Eluate highly concentrated in DNA and RNA for a wide range of
    downstream applications, to detect low concentrated targets
     Eluate with high quality, without any inhibitors for efficient and
    accurate, sensitive detection of targets

    Rapid, Easy & Flexible


     Only one set of reagents
     Easy Automation with Magnetic silica particles
     Rapid and Efficient Washing steps
     Magnetic process allows:
    ♦ High Throughput Routine Process
    ♦ Workflow optimization
    Optimization
    Frank SIMONS | Slide 10
    Marina Perrotte, 08/2007
    Frank SIMONS | Slide 11
    Marina Perrotte, 08/2007

    You might also like