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    Paraffin Wax Impregnation

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    Elysa Dela Cruz
    1. The document discusses techniques for tissue processing including paraffin wax impregnation and infiltration using solutions like celloidin and epoxy resins. 2. Different sectioning, staining, and microscopy viewing techniques are also covered such as using floation baths, adhesives on glass slides, hematoxylin and eosin staining. 3. The roles of mordants, decolorization, and metallic impregnation in staining are explained. Proper terminology for stains and staining results is emphasized.

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    Paraffin Wax Impregnation

    Uploaded by

    Elysa Dela Cruz
    19 views53 pages
    1. The document discusses techniques for tissue processing including paraffin wax impregnation and infiltration using solutions like celloidin and epoxy resins. 2. Different sectioning, staining, and microscopy viewing techniques are also covered such as using floation baths, adhesives on glass slides, hematoxylin and eosin staining. 3. The roles of mordants, decolorization, and metallic impregnation in staining are explained. Proper terminology for stains and staining results is emphasized.

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    Lecture_8

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    1. The document discusses techniques for tissue processing including paraffin wax impregnation and infiltration using solutions like celloidin and epoxy resins. 2. Different sectioning, staining, and microscopy viewing techniques are also covered such as using floation baths, adhesives on glass slides, hematoxylin and eosin staining. 3. The roles of mordants, decolorization, and metallic impregnation in staining are explained. Proper terminology for stains and staining results is emphasized.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    19 views53 pages

    Paraffin Wax Impregnation

    Uploaded by

    Elysa Dela Cruz
    1. The document discusses techniques for tissue processing including paraffin wax impregnation and infiltration using solutions like celloidin and epoxy resins. 2. Different sectioning, staining, and microscopy viewing techniques are also covered such as using floation baths, adhesives on glass slides, hematoxylin and eosin staining. 3. The roles of mordants, decolorization, and metallic impregnation in staining are explained. Proper terminology for stains and staining results is emphasized.

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    You are on page 1of 53

    Lecture 8

    Paraffin Wax Impregnation

    bakit hindi >60? shrinkage and hardening


    paraffin oven 2-5

    Lecture 8 1
    Lecture 8 2
    dapat always filtered before use

    pwede 2x gamiting

    Lecture 8 3
    paraffin only used 2x

    Lecture 8 4
    manual or hand

    ilan changes ng wax ginagamawa 4x with 15 mins interval

    Lecture 8 5
    immerse in medium for approx 3 hours

    Lecture 8 6
    Lecture 8 7
    Lecture 8 8
    Lecture 8 9
    Lecture 8 10
    lower MP
    sliding sledge ester

    Lecture 8 11
    kasi water sol = bawal dumikit sa basa kasi matutunaw
    cannot be floated out on water use other solutions

    Lecture 8 12
    Lecture 8 13
    Lecture 8 14
    Dry eyes ni Gilson

    Lecture 8 15
    gilsons mixture sa dry pinapatak lang hanggang mamuo
    sa wet celloidin nilulublob

    Lecture 8 16
    Lecture 8 17
    double embedding = CP kasi celloidin paraffin
    ICEP
    Infiltrate with celloiding and Embed with Paraffin

    Lecture 8 18
    1. L pieces can be adjsuted

    Lecture 8 19
    Lecture 8 20
    Lecture 8 21
    Lecture 8 22
    Lecture 8 23
    anong pinagkaiba nila magkaiba size ng molecule
    pinaka maliit ay spuur
    medium glycerol

    bisphenol largest size ng molecule


    mas maliit ang size ng molecule mas mabilis mag infiltrate
    spurr mas mabilis

    Polyester 1950s

    mas maganda epoxy

    Lecture 8 24
    Trimming

    Lecture 8 25
    2 kinds of trimming
    coarse kapag pangit dimension ng tissue

    Lecture 8 26
    Sectioning or Cutting

    Lecture 8 27
    Lecture 8 28
    Section cut is picked by:
    after cutting → transfer to floation water bath
    camel or squirrel or stable

    Lecture 8 29
    Lecture 8 30
    floation 30 seconds or less pag tumagal masisira
    45 - 50

    Adhesives
    hindi na daw kelangan as long as chemically clean glass slide
    ginagamit if not sticking to slide

    Lecture 8 31
    Lecture 8 32
    Lecture 8 33
    Lecture 8 34
    Lecture 8 35
    1. xylene = milky
    repeat using aOH

    clearing = dealcoholization

    first = fixation problem


    hardening of tissue 2ndary aim
    insensitive to subsquent process

    Lecture 8 36
    infiltration = airholes = repeat impregnation

    4-6 micra 5th paraffin celloidin 10-15 micra


    6th if disposal blade = replace blade; conventional steel knives

    Lecture 8 37
    to increase humidity blow or boil water to form steam??

    Lecture 8 38
    terminologies
    stain itself
    staining result

    Lecture 8 39
    mordant important sa hematoxylin

    without mordant = hematoxylin not possible


    examples

    Lecture 8 40
    Lecture 8 41
    not essential compared to mordant

    kahit wala may color parin


    used both for indirect

    Lecture 8 42
    walang differention na nagaganap
    example
    H and Eosin for frozen sectioning

    Lecture 8 43
    exampe: routine H and E
    meron decolorization

    Lecture 8 44
    most common decolorizer: acid alcohol (HCl and ethyl aOH)
    used for regressive staining technique using routine HE

    Lecture 8 45
    kung ano color stain ganon din color

    example: yellow tapos nagiging red yung tissue

    Lecture 8 46
    responsible of conversion of color to blue or blue black
    use weak alkali solution

    tap water weak alkali

    Metallic impreg
    using colorless salts reduced to black deposits on surface of tissue

    Lecture 8 47
    1. silver chloride

    2. gold chloride

    not absorbed sa surface lang

    Lecture 8 48
    picro thionine for caliculi
    cryptococcus = india ink - negative stain

    Lecture 8 49
    Lecture 8 50
    Lecture 8 51
    Lecture 8 52
    Lecture 8 53

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