Divine Word College of Legazpi

School of Education, Arts and Sciences

BIOCHEMISTRY
FINAL EXAMINATION

I. Multiple Choices. (1 point each)

1. Which of the following amino acids is the most soluble in water at pH 7.0?
a) Glutamate
b) Tryptophan
c) Leucine
d) Tyrosine
2. The folded states of globular proteins in aqueous solutions are stabilized primarily by ______.
a) hydrophobic interactions
b) peptide bonds
c) phosphodiester bonds
d) ionic bonds
3. Certain restriction enzymes produce cohesive (sticky) ends. This means that they:
a) Cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT
content.
b) Make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA
protruding.
c) Cut both DNA strands at the same base pair.
d) Stick tightly to the ends of the DNA it has cut.
4. For protein synthesis, an amino acid needs to be attached by its ______ group to the _____ of the tRNA
molecule.
a) amino; phosphoryl group on the 5'-end
b) carboxyl; phosphoryl group on the 3'-end
c) carboxyl; hydroxyl group on the 5'-end
d) carboxyl; hydroxyl group on the 3'-end
5. The basis of precipitation of proteins by ammonium sulfate is best described by which of the following
statements?
a) Proteins are rendered insoluble when they bind the ammonium ion.
b) Proteins are rendered insoluble when they bind sulfate ion.
c) Addition of ammonium sulfate adjusts the pH to the isoelectric point of the proteins.
d) Ammonium sulfate binds water molecules, making them less available for hydration of proteins.
6. Proteins can be stabilized by bonds that could include the interaction of the side chain of a lysine residue with
the side chain of ________.
a) Gly
b) Arg
c) His
d) Asp
7. Peripheral membrane proteins:
a) Penetrate deeply into the lipid bilayer.
b) Can only be released from membranes by detergent treatment.
c) Behave like typical soluble proteins when released from membranes.
d) Are generally bound covalently to phospholipid head groups.
 Divine Word College of Legazpi
School of Education, Arts and Sciences
BIOCHEMISTRY
FINAL EXAMINATION

8. An integral membrane protein will commonly be solubilized by extraction with:

a) A buffer of alkaline or acid pH.
b) A solution of high ionic strength.
c) A chelating agent that removes divalent cations.
d) A solution containing detergent.
9. In glycoproteins, the carbohydrate moiety is always attached through the amino acid residues:
a) Tryptophan, aspartate, or cysteine.
b) Asparagine, serine, or threonine.
c) Glycine, alanine, or aspartate.
d) Aspartate or glutamate.
10. Which amino acid would be released if the intact peptide was treated with a combination of trypsin and
chymotrypsin?
a) Lys
b) Phe
c) Glu
d) Met
11. Which of the following is a characteristic of both triacylglycerol and glycerophospholipids?
a) Both contain carboxyl groups and are amphipathic
b) Both contain fatty acids and are saponifiable.
c) Both contain glycerol and ether bonds.
d) Both can be negatively charged at cellular pH.
12. Which of the following is a characteristic of both waxes and terpenes?
a) Both can contain an amino alcohol.
b) Both can contain a fatty acid.
c) Both can be non-saponifiable.
d) Both can contain oxygen.
13. Which is a characteristic of sphingolipids?
a) They all contain a fatty acid joined to glycerol.
b) They all contain a long-chain alcohol joined to isoprene.
c) They all contain ceramide joined to a polar group.
d) They all contain a carbohydrate joined to a phosphate group.
14. Which is a property of eicosanoids?
a) All eicosanoids contain three conjugated double bonds.
b) All eicosanoids contain arachidonic acid and sphingosine.
c) Prostaglandins and leukotrienes both contain a ring structure.
d) Thromboxane and prostaglandins both contain a carboxyl group.
15. Based on its structural similarity to other lipids, this lipid most likely functions as
a) A membrane component.
b) An energy storage molecule.
c) A sex hormone.
 Divine Word College of Legazpi
School of Education, Arts and Sciences
BIOCHEMISTRY
FINAL EXAMINATION

d) A vitamin required for vision.

16. Which property can be shared by this lipid and a terpene?

a) Both can contain isoprene.
b) Both can form micelles.
c) Both can contain a saturated fatty acid.
d) Both can be very hydrophobic molecules.
17. Which of the following statements about nucleic acids is false?
a) Nucleic acids are polymers of nucleotides.
b) Nucleic acids are linked together via phosphodiester bonds to the ribose or deoxyribose sugars.
c) Nucleic acids are poly anions
d) none of the above (all of the above statements are true)
18. You have a double stranded piece of DNA with 15% G. This DNA would be ___% A
a) 15
b) 35
c) 42.5
d) 70
19. Heat-denatured DNA exhibits ______ UV absorbance as compared to double stranded DNA. This effect is called
the __________
a) increased, hyper chromic effect
b) decreased, hyper chromic effect
c) identical, annealing effect
d) increased, renaturation effect
20. Because DNA is a highly charged polyanion, its stability to heat denaturation
a) does not depend on hydrophobic interactions

b) increases with increasing salt is

c) independent of G - C content
d) decreases with increasing salt
21. Which structural feature is shared by both uracil and thymine?
a) Both contain two keto groups.
b) Both contain one methyl group.
c) Both contain a five-membered ring.
d) Both contain three nitrogen atoms.
22. Which property is shared by both GDP and AMP?
a) Both contain the same charge at neutral pH.
b) Both contain the same number of phosphate groups.
c) Both contain the same purine.
d) Both contain the same furanose.
23. Which characteristic is shared by both adenine and cytosine?
 Divine Word College of Legazpi
School of Education, Arts and Sciences
BIOCHEMISTRY
FINAL EXAMINATION

a) Both contain one methyl group.

b) Both are anomeric.
c) Both contain one keto group.
d) Both are heterocyclic.
24. Which characteristic is found in both purines and pyrimidines?
a) They both have aromatic rings that undergo substantial tautomerization at neutral pH.
b) They both are weak bases that can be positively charged at neutral pH.
c) They both have multiple pKa values that result in zwitterion forms.
d) They both can form stable N-glycosidic bonds with β D-ribofuranose.
25. Which is a general property of both nucleosides and nucleotides?
a) Both contain a pentose in the form of a furanose.
b) Both contain at least one 5’-phosphate group.
c) Both contain a nitrogenous base that forms covalent H-bonds.
d) Both contain a hemiacetal or hemiketal bond.
26. Which of the following double-stranded DNA molecules would denature at a lower temperature than the 40
base-pair double-stranded molecule described above?
a) a 40 base-pair molecule in which 25% of the bases are adenines
b) a 30 base-pair molecule in which 40% of the bases are guanines
c) a 20 base-pair molecule in which 10% of the bases are thymines
d) a 10 base-pair molecule in which 20 % of the bases are cytosines
27. Which characteristic will this double-stranded DNA molecule share with a double-stranded RNA molecule of
the same size?
a) Both will have secondary structure.
b) Both will contain inverted repeats.
c) Both will be degraded by base.
d) Both will contain four types of base-pairs.
28. Which will be a characteristic of this one single strand?
a) The single-stranded chain will contain both ribose and deoxyribose.
b) The single-stranded chain will contain both purines and pyrimidines.
c) The single-stranded chain will contain one 5’-end and one 3’-end.
d) The single-stranded chain will contain multiple phosphodiester bonds each linking a 2’-carbon and a
5’-carbon.
29. When this single strand binds to a complementary DNA strand to form a B-DNA structure,
a) Half the base-pairs will be A-G pairs and half will be C-T pairs.
b) Each base will form at least two hydrogen bonds with a base in the opposite strand.
c) The two strands will form an antiparallel left-handed helix with 12 base pairs per turn.
d) there will be covalent phosphodiester bonds between the two strands
30. Which are characteristics of bacterial genetic material?
a) It is double-stranded and supercoiled.
b) It is single-stranded and relaxed.
 Divine Word College of Legazpi
School of Education, Arts and Sciences
BIOCHEMISTRY
FINAL EXAMINATION

c) It is circular and compacted into palindromes.

d) It is linear and attached to a protein scaffold.

II. Identification (2 points each)

1. Responsible for specifying the 3D shape of a protein.
2. Average molecular weight of amino acid residue in a protein.
3. Protein that was first sequenced by Frederick Sanger.
4. Number of amino acids that make up a protein.
5. Cell organelles involves in the process of protein synthesis.
6. A disease caused by protein deficiency.
7. Cell organelles where protein synthesis takes place.
8. A form of lipids referred as neutral lipids.
9. Beta oxidation of fatty acids occurs in _______.
10. The specific gravity of lipid.
11. Group of adjacent nucleotides are joined by ____________.
12. The sugar molecule in a nucleotide.
13. Building blocks of nucleic acids.
14. A nucleotide bases that can’t be found in RNA.
15. It initiates the unwinding of the double helix by cutting one of the strands.