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    Exercise 3 Microbiological Culture Media PDF

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    Sittie Ainna A. Unte
    This document is a laboratory worksheet for a pharmaceutical microbiology class. It contains exercises on preparing solid and liquid culture media. The exercises include calculating amounts of dehydrated medium needed to prepare plates and tubed media, illustrating different types of solid tubed media, providing examples of applications for each type, completing a table comparing nutrient broth and agar, listing microorganisms that can be grown in broth, and discussing the implications of omitting a spore strip during autoclaving preparation.

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    Download as PDF, TXT or read online from Scribd

    Exercise 3 Microbiological Culture Media PDF

    Uploaded by

    Sittie Ainna A. Unte
    232 views2 pages
    This document is a laboratory worksheet for a pharmaceutical microbiology class. It contains exercises on preparing solid and liquid culture media. The exercises include calculating amounts of dehydrated medium needed to prepare plates and tubed media, illustrating different types of solid tubed media, providing examples of applications for each type, completing a table comparing nutrient broth and agar, listing microorganisms that can be grown in broth, and discussing the implications of omitting a spore strip during autoclaving preparation.

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    Exercise_3_Microbiological_Culture_Media.pdf

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    This document is a laboratory worksheet for a pharmaceutical microbiology class. It contains exercises on preparing solid and liquid culture media. The exercises include calculating amounts of dehydrated medium needed to prepare plates and tubed media, illustrating different types of solid tubed media, providing examples of applications for each type, completing a table comparing nutrient broth and agar, listing microorganisms that can be grown in broth, and discussing the implications of omitting a spore strip during autoclaving preparation.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    232 views2 pages

    Exercise 3 Microbiological Culture Media PDF

    Uploaded by

    Sittie Ainna A. Unte
    This document is a laboratory worksheet for a pharmaceutical microbiology class. It contains exercises on preparing solid and liquid culture media. The exercises include calculating amounts of dehydrated medium needed to prepare plates and tubed media, illustrating different types of solid tubed media, providing examples of applications for each type, completing a table comparing nutrient broth and agar, listing microorganisms that can be grown in broth, and discussing the implications of omitting a spore strip during autoclaving preparation.

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    PHARMACEUTICAL MICROBIOLOGY LABORATORY WORKSHEET

    UST Faculty of Pharmacy

    Group # _______
    5 Member # _______
    4 Class # _______
    20
    Name _____________________________________________________________
    Lee, Abigail Junuea Section _______
    2I-PH

    EXERCISE 3
    MICROBIOLOGICAL CULTURE MEDIA

    PREPARATION OF SOLID PLATED AND TUBED MEDIA


    Nutrient Agar container label

    Directions: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the
    medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to
    45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids.
    Mix well and pour into sterile Petri dishes.

    1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates.
    Include amount for 2 additional plates as excess to compensate for compounding losses.

    2. Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and
    25 butt-slants. Include amount for 2 additional units of each as excess to compensate for
    compounding losses.

    3. Illustrate the different solid tubed media formed.


    Deep/Butt Slant Butt-Slant

    D- OF
    This study source was downloaded by 100000848616065 from CourseHero.com on 09-04-2022 08:13:46 GMT -05:00
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    PHARMACEUTICAL MICROBIOLOGY LABORATORY WORKSHEET
    UST Faculty of Pharmacy

    4. Give examples of specific applications for different types of solid tubed media
    ___________________________________________________________________________________________________________
    Deep/Butt is used for motility, the tube of agar is stabbed into the agar without touching the
    bottom of the test tube. This is to observe the movement of microorganisms. Slant allows greater
    ___________________________________________________________________________________________________________
    surface area of microorganisms to move around, which is helpful in maintains the bacterial culture.
    ___________________________________________________________________________________________________________
    Butt-Slant on the other hand allows the culture media to grow in both aerobic and and anaerobic.
    ___________________________________________________________________________________________________________

    PREPARATION OF LIQUID MEDIA


    Nutrient Broth container label

    Directions: Suspend 13.0 grams in 1000 mL distilled water. Heat if necessary to dissolve the
    medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs
    pressure (1210C) for 15 minutes.

    1. Complete the table


    Parameter Nutrient Broth Nutrient Agar
    Composition - Simple peptone - Peptone
    - Beef extract - Beef extract/ yeast extract
    - Solidifying agent

    Applications Cultivation of different Used to cultivate and support


    variety of fastidious and non- growth of broad ranged of non-
    fastidious organisms fastidious organisms

    Sign of microbial growth - Turbidity Visible growths of colonies


    - Precipitation and pellicle
    formation

    2. Give 5 examples of microorganisms that may be grown in Nutrient Broth.


    ___________________________________________________________________________________________________________
    The five examples of microorganisms that may grow in the Nutrient Broth are:
    Streptococcus pyogenes, Psdeudomonas aeruginosa, Escherichia coli, Staphylococcus aureus,
    ___________________________________________________________________________________________________________
    and Salmonella Typhi.
    ___________________________________________________________________________________________________________
    ___________________________________________________________________________________________________________

    3. Discuss the implication/s if spore strip was missed out during the preparation of sample for
    autoclave and possible remedy for this scenario.
    ___________________________________________________________________________________________________________
    If the spore strip was missed out during the preparation of sample for autoclave, it would
    be difficult to identify whether the sample has been completely sterilized. A way to fix this
    ___________________________________________________________________________________________________________
    situation is to incubate the sample for 24 hours and if no apparent organism grow then it has
    ___________________________________________________________________________________________________________
    been fully sterilized.
    ___________________________________________________________________________________________________________

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