Experiment 1. The Compound Microscope

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NAME: KENNETH G.

ROMERO GROUP NUMBER: __________


YEAR AND SECTION: CCR3-B3 DATE PERFORMED: 09/01/2022
PROFESSOR: MARRY ANN DEOGRACIAS DATE CORRECTED:

EXPERIMENT 1
THE COMPOUND MICROSCOPE

I. OBJECTIVE: TO STUDY THE PARTS AND THE CORRECT TECHNIQUE OF USING THE
COMPOUND MICROSCOPE.
II. MATERIALS: compound microscope, glass slides with specimen

III. DISCUSSION: The compound microscope is an example of a light microscope, a type that
makes use of visible light. It consists of two sets of lenses, namely, the primary magnifiers or the
objectives, the lenses of which are immediately next to the specimen being studied; and the
secondary magnifier, that is, the ocular or eyepiece, the lenses of which are next to the eye.

IV. PROCEDURE: Draw and label the parts of the compound microscope.

V. OBSERVATION/CONCLUSION:

1. Give the function(s) of the following:

a. ocular/eyepiece – The eyepiece, or ocular lens, is the part of the microscope that
magnifies the image produced by the microscope's objective so that it can be seen by the
human eye.

b. revolving nosepiece – This is the part that holds two or more objective lenses and can be
rotated to easily change power.

c. coarse adjustment knob – A rapid control which allows for quick focusing by moving the
objective lens or stage up and down. It is used for initial focusing.

d. low power objective – Low power objectives cover a wide field of view and they are useful
for examining large specimens or surveying many smaller specimens. This objective is useful for
aligning the microscope. The power for the low objective is 10X.

e. high power objective – The high-powered objective lens (also called “high dry” lens) is
ideal for observing fine details within a specimen sample. The total magnification of a high-
power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving you a
very detailed picture of the specimen in your slide

f. stage – The flat platform that supports the slides. Stage clips hold the slides in place. If your
microscope has a mechanical stage, the slide is controlled by turning two knobs instead of
having to move it manually. One knob moves the slide left and right, the other moves it forward
and backward.

g. condenser – the condenser is located beneath the stage and serves to gather wavefronts
from the microscope light source and concentrate them into a cone of light that illuminates the
specimen with uniform intensity over the entire viewfield.

h. mirror – it is used to reflect light from an external light source up through the bottom of
the stage.

i. iris diaphragm – The opening and closing of this iris diaphragm controls the angle of
illuminating rays (and thus the aperture) which pass through the condenser, through the
specimen and then into the objective.

2. Describe briefly and in a systematic order, the manner or method of properly using the

compound microscope.

- The systematic order of properly using a microscope starts with the handling. You should carry
it with both hands with one hand supporting the base. When you put the microscope down, put
your specimen on the slide and fasten it. Look through the eye piece and adjust the lens as low
as possible but without touching the slide.

3. Give some precautionary measures in the use of the microscope.

- Do not touch the glass part of the lenses with your fingers. Use only special lens paper to clean
the lenses.
- Always keep your microscope covered when not in use.

- Always carry a microscope with both hands. Grasp the arm with one hand and place the other
hand under the base for support.

4. Name the three general parts of the compound microscope and their sub-parts.

* Head/Body houses the optical parts in the upper part of the microscope.

* Base of the microscope supports the microscope and houses the illuminator.

* Arm connects to the base and supports the microscope head.

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