Forensic Toxicology
Forensic Toxicology
Forensic Toxicology
Poyeicin Pornceltu
loxiedlgay is fhe
studa of por tans
It ic concernesd ith ne chemical ond physical propertier of toris tuasto.
Ces 0d fheir physio logi eal effechs on living9 orgónism 7he qualtriun
n quantifoti ve methode por their anglusie in bióbgical ond nn- bit lgra
aferiols and the develop menf of procedures for me retmest of poit
ning
ASl1he
por&en is ang aubstance ohth token in afpieie quoni ail oue
aHh or death
forensic boxricolpgist it primarily coocerned aith he defachion ael estimatios
F P isons in tisues ond body tloid obfained at autopsy , or occagsioel
In_ blood urine_or gagric marerial obtained from
,
a
hvun4 person
Drugc abuse
Drug is'a substance ohich may have medical, intoxicatip. pepormat
entancing or other effecks ohen aken_0r_put into honan badyu or he hody
of anothér animal andis not considered foo
>h drug that is taken for non mecical reatone lusually 4or i - alerip
ee s dyg of abus Drug obuse con lead da husical and meotal
damage and loith some substaoces) depeneene and addichion
Drug abuse is a uce of drug or substhnce in ohich con s umer taks
The substance io such quaoi or by usin9 such mehodr whith are
neaher superviced nor appra ved b an4 medical prafeccional
Ll1.
9 ng abuse is nt restricded to psyeho-attve er moodl changing drg
Some substance is used ifh an objective fhaf is agains the politr
and rules of he place los stereidc ore
used in
Spots effr ehh ecing
pespor mance), His alco called o9 sukvante a buke
Clasaiaicahoo
ladioistratrd by mau h
Akaqe sedahive dase aTbihmto y 16-90 mg
A. Based on
origin
atural Drus: derivedArom dnd reqluire li7Hle or.O0proce
Ecannabis,opiumoca pasTe
emi-cuothetic produter bu suoecting ome chemal poE
S9cocainec ,herain
ohehic iChemicol procesu are the sole bapis at produchion dg 1hee drug
melth aualone _Inandbax), amphetomine Hype sthimulanks ,dazpin,
8-Based net they-produce
Tim.ulant pychoaekive drugs that mduce temporay_imprevenmeDAS_In eith
menfal or phASicol cunctigns or both ioclude kuhstonces That wake one u
,
fulness,
,
ek
Chrnic intoxicatioo :Sleep problem, supprescion 4 oppefite
kass ef appefite Mood
Rapid nd ireguar_heart beat
,
dioiggshmphetasie
i.Aitdraal ymphame Na ,elevote B PSehog
mas
Ho Severe iveysiolbg itel ailure, distu rhed sleep ,moderde
depression ,Exireme fatia ue
Deprescant i dnuanere): includes all he
Yeducinganecihetic of
cQuplee NIh substance) uhich may induce relaxingcolmative ,anie
change in perreption
ike alahol tophotbarbitursts feelicas: Alcohol
create a feling o oc~ on_ CNS o
sress Barbi
Tte turate4Benzodiazepire
sheep.. violfnchions so rela
ell being_and prduce
kong_aching
Shot harbiturakeobsabed more chaly (Phenabarbida!
abtorbedather quickly (Peiokarb
in! ec
kOace the ballutinogen 1s in your body, chemitol Ike cerotonin and aceleholine
hallutin atianu (tan lay 3044 h»)
ntefere ifh neurotransttens on caute
DMIdimeyl4ryphmine ) peilybia
iocludej tuens
3Malutinggens produre fhat alH#eratons in oomal thought prncees,perrep.
diehinet
ion od ma0d, a lHer sontou proesting in the brain
a
Peyehedelies q.4sD,
SOtsergi
Peyeh mescaline
acid diylamide) is cynthe sized trom lytegic aud,a mbstane
deived m erget la ype of pungur that ottark cenain pe f grains)brs.
1his i poteok rug. 0oly 2S cufticient do cause halatindtiont for 12
bDiccociative lg: Mogic muchroo tamim, PCP)
PCP(phenyleyelidine is sypthesized sinple chemical procels. i s e t
mIyed oi fh other drugs like ASD or omphetamine and is sald a e peoder,
copsues or tobleh or y lig wie
eriants£g, Dabura JisenWeed
4
Narcei cs: -
Su hsrtanes thot act
CNs and bripg reliep fron poin ond
an
(diatylnerpwne)
impure form-) brownSy907
10-1 km more potent fih on morphine
pium o y nil juie obd from unnpe pod pope lbpaver soma orm)
Symp toms
Euphona, Drauginas &apathy , Impoined reasoning; conuukion, comgdesth
(over dare heroin)
Feli ef unaell penistenf body poin , lasomnia.
ni' oll efftt lHinmulatons, depraand, helcinageng,narcohe?)
S Loonabis : lbooobit plonks predute a rap chemicols knoun.os Coongbineid
u hich pradutey mefal ond phycical efecfs when takoo
At .0
a ut
drug, usuall tomes
infarm o dned tlouer buds (neijanel, reuin (ren
r vorio ur extrac colechuel knsan o hashish oil,
deophor1a, Akemd sense,Impaired nemo,Bor mshr séille
foor cosoihve skils , vespi mhy problems,impotence yehoi
Slep dshurban ces , Irikabli, Trema EDeprestio
C.Ve lahle sàleenk Pe re leam derivaivs &ve lahile HC.
oreosic Toxit olag
#Concep-
-1 is the applicahion of Con vetional toxico lpgy for The puparer the crhni.
nal inveshgation to ásos legal adminisrations
od urdanerhl dori
1t cendidered
is as a hybrid o Anauficol themisy
d ih advaneed brensleh médieine ericties t the debimeehu
i s primoril coeened te medico- legal charac
ec o echemitals humoo md oimals
an
tora ace)
Aolloe ing sampler muei se collected and prese rved
Stooath and uhole at iks conrenhe (or stomech aasheiher_is avalable ono
one feet af provimal part of cmall intenshine albpg wth its Ontenk2
2504 o kiver Jpreteraby porhian contaioipg goll blaoer and ih ctent halp
ofeach krdney)
Bhod 50 mEn case_of akohol tofher tolaie paisan
poisonipg Cd poiconiag
-
Containe4
'reseruath0o
Cose Maleriak to be sent Pruervnhv th be uwed receruatives of
USe d
Bpo
Vistera mast be_preserved_ia sauraBed aCl_sslation. bol must be
preserved in 1 Sodiun floride solution. n case of alcohol paieig
ánd poicooing bu valatile poisens ,s ample shouldn be_preservedin
a lcdhol c cera couldbt_be precbrved in recttied tpinit or foumalin
h case of_acid poisooing, vis terd sbauldot_be prereredin coninan t
pluhonasttead rechpëd splrik can be_uced_be precervafives
Dformation nhe includes alopg oh sgtnple_t taxiralggical_exhibi n caeg
Namee, 0ejght and haighi at deod sertoh.
ed ate lat ime
- Ater aba hauootrmueh tine q eating doe pm poisonirg as ohoerved
-
Vamited_or 1
- blasthe pescen tpcanscioas ar hejshe has menthonee pain er untenfart in ay
the person uas sick ,uhich medicine wns gien1 parf)
-Alame opoisons cited by dead pertonAr_Egewigheised
- Name e kuspected poitoH ineludt oble, ba?tle taniner if pugd near vicin
phaken h ne hspital,ipclude dhehgil t remnol give.hainclue me
Colle thion Jordoth noy, bloo and rine.
- Alditisnal injarmahoa (it vichim is
pregnun, ottupafios t,and oher peeons!informakon
4bel 9boukdeod perkenton dher peopk
Clastifadioa opoitooe in sufticieed amt
-
Catspich o
onn be dohe octording f6 mde at ot hen 44eheie
af paicear
Mode af oction helpe in deferminigg the tape of polton ohd chenicol netur
eature
poic on bepe in anayrig I.
A Rased on mode ef action
Cerres ire poitons:IH te highly active initant and ot only preduees toglo-
mmatian bot alsa octual uleeraBion_of the tissues. Basicao comasive poi
ves,
destroy and ersdes the surpaté with ahich ih comet 'n tonkac! ieh
redeces ocal acfionhey act by extrocting oater from fhe titaves and
gulefe cellular proteins and converf emaglo bin tnho hematin.
ngroup congich a &hropg acide ond shopg ilkalitHsoaHD,,He!,
Dx l i e acid carbolic acids, oaliculic acid, NabH,corbenatas,ommsnium,Na,
sen
a
Neuretier puicons ah cheth en nervou en hgh 1ome nturhe
a lacal miaat action Fg.alkahid poitangbisroton.cir t pal
lcor
thet bave dpengic action on the cerebua spinal 4peripherdl neu
he paitobeng kron_a serehrol,spinal épenphtril racp
Cerebml poiconmg have a somnigeeo ley gpiads) inebrist ( ol
an0Rstheties,dedhhves Lhupnabics fuelsarochernital ampounds)or delirig,
effect (hy dhotarnbeladsone,conmab
Spinol poionr include_nux Vomica and ik alkalbids and gekeian
Peripheral poisong include curare_and conium
b
Larciat paitans i..ach onthe heak inchuderdigikalie akander
aconite2nicoine
C
Asphuyior poisons-octr on tha recpiratory syetem. It intludeu ieipin
Aouch os CO, CO hwes tome_o4r Jade
seLwer
dgtelaneous poisons hext are poison having diteengd
ntlades
phormocobia
1 dnolgesties Aotipy refies onhihismnicdranquitzsr ,0iat
s:ontt, streel drgr desianer
druge
2asec00_00ufepaiton
1 Gasedus poisons ilhese are_in gaseous state and i iahaled ,hamper
Competen te at blaod as a_tarrier a oxgen_ond mgs domoge the kisue
of fhe air patsqges ond lurgs. Co, co M,S , So2 cI,Ng0, tear,
Vplatile pogoni poi sont: -Acute pois ening_aith vo latile substanter
urua
follb os th e slbuo inholation of
vopous Yn order to became intoxitaked. anst
phosphine,arsine photgene chloride , edt.
9.Volahile Ormnic hoirons-ae oganit chemkalk that have high VP ot ordinary
a
Suicidalpaisaning
Intake e tome poisanaus substonce by indiuidual himcelp for selp_ kilin
Joealsuicical poicons chould be easilg auailable euce ná pain o7 es pan
Cheap Hegtelecr or plea.mni tasfe copable op heipg da ken' wth food or dhink
Include opium abiturahe drganophasphorusdaral acid,CusoG_ , vt pest
Lontaining inc phasp.hide mic acid
3-Accidental poisenáng
General hke place due do storoge opoisonouS 4 Don-p0isopous aubston ot
thesame place.
Noticeable acidenhal poisons_inclade ospininonanphasphottus,Caso, sele
biHesrgat,Co,CoH,et
aFoo poisoningocor oue n lock s Santafion public hogiene
can_ occur in man ays and mabe 16olated instonces_or may constiare
m epidemi mase and poiso.ning ögital caues_are micrabd, 2ans
fungi, plants animals,eft. and na tur due chemitol ogeh inkrodere
duwng 4ood preesting like heaug meals peshiidestosd addive
larhthidans 'HaveHog qen, cdleurliny agen, ruettgg ajen ,preservahve
L hogenic poisronig arises from the action af dactorr ,for instonce
iniechon af ong otuHiDn or of'oan appropake coluhon in gn on moppropr Ae
xraetion is a
very common la horatory procedore using for isela
octen (diguid- iguid oxtrachon) is me bacic pejerned
mebe as i is e conomical and e asy Ho pezorm
he xraehion of the analyhe is baged dn the traneer ofo l
Jubstaote from oneliqu id phose into another hiq. phase bered U
he etra cion tolveots are organic elatile andimiaéible ia th ahe opem
layer and he taa layers are dishind/ separafed
orgaoie lyer is pacred through onhydiout'sodhum tuphore to Rnae ip.
The dps poison dhich diccoe infa orante calver ave leth pr eva
pordio, ths fhe pure dugl poison ís exfracteol
daigslpoICDsron viccerc
re- treatmeot
50_tieue in 1o0 ml beaker ouf ioto emallpieces macerafedprapery
material was tronsfered h he plask (conienl28) uith 10g mkgro ur
Jedlum Sulphake and 10 mo4 gloaial acetic_acid
he lask was placed on baili water baih af 60'C 4or 8-9 haune
-
Cn fents are then cobled ahdl piltered ucing iltr paper. 1he piltrate wa
utea jor xtractio n ot dif. drgs paicon~ åt pek folloaing methodr
Basic ertroctioo
etratted with 2Sml, 20m4 n l ether: chlarofarm 3:1) mittur
Neuteal ex troction
vtrattead oith sml, 20nl 41S mi chkxo fasm
Acidic extroction
10 mi
urine comple wos mode ocidic by addiog HPO% ar tototie.
-
fupnel-
eyefher_war
T10m 10m ethe) p) 4ded to it and shaken'por S-0 oi'ub
9m
VireousHumbar t1Sol op ehe oddes 4 sholken fu s-,
,nit loyer,
(Aeie erfrot)- -Made olkelimeAguepus
laifth
layer
ommooium
Dsed tor identizicatinn
o_acisit dmg) iS ml chloroform :ether (1:3) hydrny-
hve
added 4 chaken for S-10 mio
Organic tayer Aqueous laye
(Basic extrati)
(ked for idenfigicohsn basic Made
-1S ml
neutral with glocial octhies
drugs) chlsrofotm &Aded 4shokeni-
S-L0 miquh
Organie loyer
tNettra erwatt)
Aqueous layer
-Used (Dir cardey
por identiticatioa q neural dy
Tdeot ah on techn igue
HIcn te riue
les preeie
#Praam
Prasunivt at f, Nortohis drugs 4hodrgpic dubitana
hive tesk indiate that the sispectescuhstonre je preamt o
nat. is wulcolazimetic lielcolaur fort).
A presumpfive Hest forillicik drupsi based on spot tesks Thot pod
ncespekigic celors for specipie duge aldo inelueas the
sical examinadion_(iualor micrascopic eroninatioa) 4o uodexkand
phgsical praperties ond charatterictics ot suaetoncas.
1 Opium
A Marquis test > Bmoll amt a[Cuspected moteial +3 drops of woter
-Ad a drop of teagei 1-0Sml o4 0/ HeHS to 1d ml ar glaciolg
Adedráp ot ont.H,$ D
Purple to violet colour indicates_ postibe presente _o;_opium
amattrial+3drsp ot ater)+ Ldrap Fesia
3.ene solphare Fest9 brownish purple _colour tnelcafe pres ence of opium
3.Canabis
A. fost Bue_B Sll Test
- smallamd. of material in tast the +mall ont-o reogeed 1/p d L
+sdrop of chbrototn, shake fest tuhe or 1 ir ¥daplaokyenus o,
01N Na0M Soluhon, shake #or mihufes
Purple -red colotur ot the lower(ehlorotwn)Jayer indicetau popst 9
he hwerlehlorojarm) laye
Violed tolor qy
kale
anoh,
c m d Hel , sheake for ew sec
ted 1sn
and allo w ixfure h
rf min color develops within 2-3 min add 2
chlbhyerm 0nd shoke the miture
nty
1 Cecaine
ACeball thia cyanafe test -> Blue colau
marerral in ttl tube 1 adrop of_16.a4. HC/
+
sol, Shake_4or .
1 orap 2S1._cobalt ) hiozuanate 'solution
ef ,shake fer
8. Moadipiesd tanl hiogyonah 1esfo best)
materíol tS drap at reaqeot 1(19 cobaf () hioguon ae iccoled
Ao blue ceiour, add a adaolant07 acetie aci9,4 so ml glgeeine
material irst I blue tobur till nat develaped,csdaine ok
as
3. Methagu alone
A. Coball thioyonaBe eblue colour
0yseride AsD)
A Ehzich test Viole! cobur_othin foo _minute
11 Mesealine
AMarguis lesk Oronge colour
B.Libermao0 Tes blbck colour 1 drop_at resgert 1 so djum aitie
18ml con uasoa
12.Peilacybine
A.Margis ask Dronge cobur .Ehhich tesk Violel cobur
13.Phencycliine
A.Cobalk hiasyeoate te! Blue tobon B.Mecke et Prk olr
19 Fentanul/ opha - nehylAeutagyl 1S Pethicline
A.MalquTS test orang ol our 4 Marg ist Hest 2 Orooge obur
B-Aibermann desd Red ony
Methadone
A-Marquis fest nk toour
develkoingaholy 4 thangiog to vie
B.HND-, tst 0sagge ) O_ red
m deri al + drep Yagayt
10drop(3m) o toe
HNO, to
lonloM&0