DH36 Auto Hematology Analyzer Service Manual - V5.0

Auto Hematology Analyzer
Service Manual
Preface
Thank you for purchasing the Auto Hematology Analyzer manufactured by Dymind Biotech.
Before performing maintenance on the device, please read this service manual carefully to gain an
understanding of how to properly maintain it.
After reading, keep the manual in a convenient location for future reference and easy access.
Product name: Auto Hematology Analyzer
Model: DH36, DH33, DH31
Contact Info for After-Sales Services

Shenzhen Dymind Biotechnology Co., Ltd.
2/F, Nanfeng Building B, Nanshan Yungu Innovation Industrial Park, No.4093, Liuxian Blvd,
Taoyuan Street, Nanshan District, Shenzhen 518055, P.R.China
Landlink GmbH
Dorfstrasse 2/4, 79312, Emmendingen, Germany
Tel: (86-755)26989825
Service Tel: 400-998-7276
Fax: (86-755)26746162
Email: dymind@dymind.com
Website: http://www.dymind.com
Intellectual Property Rights

This document contains proprietary information of Shenzhen Dymind Biotechnology Co., Ltd.
(hereinafter referred to as Dymind Biotech).The corresponding product is the intellectual property of
Dymind Biotech.
No part of this service manual may be reproduced, copied, modified or translated in any form or by
any means without prior written consent of Dymind Biotech.
.
i
Declaration
Dymind Biotech reserves the right of final interpretation of this service manual.
Dymind Biotech shall be responsible for the safety, security, and performance of the product only
when all of the following conditions are met:
 The assembly, re-commissioning, extension, modification, and repair of the product are performed
by authorized personnel of Dymind Biotech.
 The installation of the relevant electrical devices complies with applicable national standards.
 The product is operated in accordance with this service manual.
Maintenance Services
Scope of free services:
All products complying with Dymind Biotech’s Product Warranty specifications may qualify for free
services.
Scope of fee-based services:
 Free services are available to all products exceeding Dymind Biotech's Product Warranty
specifications.
 Products within the warranty period require maintenance under the following circumstances:
 Artificial damage
 Improper use
 When grid voltage exceeds the specified operational range
 Unavoidable natural disasters
 When parts and supplies are replaced with no prior consent from Dymind Biotech, or machine
maintenance is performed by personnel with no prior authorization from Dymind Biotech.
 Any failure by hospitals or organizations responsible for using this instrument to implement a
competent repair/maintenance plan is likely to result in abnormal instrument failure or even a
health hazard.
 Make sure the analyzer is only operated under the conditions of use as specified in the operator's
manual. If operated outside of the specified conditions of use, the analyzer may not work properly,
which could lead to unreliable measurement results and damaged instrument components or
even bodily injury.
This analyzer must be operated by trained medical testing professionals, doctors, nurses, or
laboratory technicians.
ii
Contents
Contents
Preface....................................................................................................................................................i
Contents .............................................................................................................................................. iii
1 Manual Overview .............................................................................................................................. 1
1.1 Who Should Read This Manual ................................................................................................. 1
1.2 Symbols and Legends................................................................................................................ 1
1.3 Safety Instructions...................................................................................................................... 4
2 Instrument Configuration ................................................................................................................ 6
2.1 Mechanical Components............................................................................................................ 6
2.2 Hydraulics System ..................................................................................................................... 9
2.2.1 Schematic Diagram of the Hydraulic System .................................................................. 10
2.2.2 Hydraulics Diagram for the Overall Unit .......................................................................... 10
2.2.3 WBC/HGB Measurement Channel ................................................................................... 11
2.2.4 RBC/PLT Measurement Module ....................................................................................... 11
2.2.5 Sampling and Blood-dispensing Module ......................................................................... 12
2.2.6 Power Supply and Waste Discharge Module................................................................... 13
2.2.7 Status Monitoring Module ................................................................................................ 13
2.2.8 Hydraulic Components ..................................................................................................... 14
2.2.9 Main Measurement Modes .............................................................................................. 18
2.2.10 Hydraulics Maintenance................................................................................................. 23
2.2.11 Troubleshooting Common Hydraulics Problems ............................................................ 24
2.3 Hardware System ..................................................................................................................... 34
2.3.1 Main Control Panel .......................................................................................................... 35
2.3.2 Other Panels .................................................................................................................... 51
3 Repairs ............................................................................................................................................ 53
3.1 Overview .................................................................................................................................. 53
3.2 Preparatory Work before Repairs ............................................................................................ 53
3.2.1 Open the Left Side Panel ................................................................................................. 53
3.2.2 Open the Right Side Door ................................................................................................ 55
3.2.3 Disassembling the Front Panel Cover ............................................................................. 55
3.3 Display Screen Assembly Replacement .................................................................................. 57
3.4 Sampling Assembly Replacement ........................................................................................... 59
3.4.1 Replacing the Sample probe............................................................................................ 59
3.4.2 Replacing the Optocoupler .............................................................................................. 60
iii
Contents
3.4.3 Replacing the Sampling Assembly in X- or Y-direction .................................................... 61

3.5 Power Assembly Replacement ................................................................................................ 63
3.6 Hydraulics Components incl. Valves, Pumps, and Pressure Chambers Replacement ........... 64
3.6.1 Replacing the valve assembly ......................................................................................... 64
3.6.2 Replacing the Liquid Pump Assembly ............................................................................. 65
3.6.3 Replacing the Negative-pressure Chamber Assembly .................................................... 67
3.7 Sheath Flow Syringe Assembly Replacement ......................................................................... 68
3.7.1 Replacing the syringe ...................................................................................................... 68
3.7.2 Replacing the Motor ......................................................................................................... 69
3.8 WBC and RBC Bath Assemblies Replacement ....................................................................... 71
3.8.1 Dismantling and replacing the WBC bath assembly ........................................................ 71
3.8.2 Dismantling and replacing the RBC bath assembly......................................................... 73
3.9 Main Control Panel Replacement ............................................................................................ 76
3.10 Reagent Testing Panel Replacement ..................................................................................... 77
3.11 Temperature Sensor Replacement......................................................................................... 79
3.11.1 Disassembling and Replacing the Temperature Sensor ................................................ 79
4 Software Upgrade ........................................................................................................................... 81
4.1 Preparation ............................................................................................................................... 81
4.2 Upgrading Steps ....................................................................................................................... 81
4.3 Touch Screen Calibration ......................................................................................................... 83
5 Comprehensive Device Tuning ..................................................................................................... 84
5.1 Position Adjustment.................................................................................................................. 84
5.2 HGB Voltage Gain Setting........................................................................................................ 85
5.3 Gain Calibration ....................................................................................................................... 85
5.4 Calibration of Calibrators.......................................................................................................... 86
5.4.1 Calibration in Whole-blood Mode ..................................................................................... 86
5.4.2 Calibration in Predilute Mode ........................................................................................... 87
5.5 LIS Connection ......................................................................................................................... 87
5.5.1 Installing LIS Workstation................................................................................................. 87
5.5.2 Host Communication Settings.......................................................................................... 88
5.5.3 Connecting Analyzer with LIS .......................................................................................... 89
6 Alarms and Solutions .................................................................................................................... 91
7 Maintenance Inventory .................................................................................................................. 96
iv
1 Manual Overview
1 Manual Overview
This chapter explains the procedures for maintaining DH36 Auto Hematology Analyzer. Please read
this manual carefully to guarantee proper device maintenance and operator safety.
This manual is complemented by the operator's manual; there is no overlap between the two.
Be sure to operate the device in strict accordance with the instructions in the maintenance and
operator's manuals.
1.1 Who Should Read This Manual

This service manual should be used by professionals who possess:
 A comprehensive knowledge of electrical circuits and hydraulics
 A comprehensive knowledge of reagents
 A comprehensive knowledge of quality control
 A comprehensive knowledge of troubleshooting
 The ability to skillfully operate this analyzer
 An understanding of basic mechanical tools and any relevant terms
 Skills for using digital voltmeters and oscilloscopes
 The ability to analyze electrical-circuit graphs and hydraulics diagrams, and to understand the
related terminology
1.2 Symbols and Legends

Symbols used in the manual:
Symbol It means
The operator should follow the instruction below each symbol to

avoid personal injury.
The operator should follow the instructions below each symbol to

prevent instrument failure, damage, or disrupted test results.
1
1 Manual Overview
Symbol It means
The operator should follow the instructions below each symbol and
pay special attention to any pertinent information when following
the procedures.
The operator should follow the instructions below each symbol to

prevent any danger of infection.
The following symbols can be found on the analyzer, reagents, QC materials, or calibrator:
When you see It means
Caution
Biohazard
Exercise caution to prevent puncture
Instruction for Moving
Network interface
USB interface
Protective grounding
Alternating current (AC)
In Vitro diagnostic medical device
Batch code
Use-by-date
Serial number
European CE declaration of conformity
2
1 Manual Overview
When you see It means
Authorized Representative in the European Community
Date of manufacture
Manufacturer
+40°C
Temperature limit
-10°C
90%
% Humidity limitation
10%
106kPa
Atmospheric pressure limitation

50kPa
Consult the instructions for use
Keep away from sunlight
Keep dry
No rolling
No Stacking.
Let this side face upward.
Fragile, handle with care
Recyclable materials
The analyzer, after being scrapped, should not be disposed

with other household garbage, instead, it should be collected
and recycled following the disposal instructions for scrapped
electronic and electrical equipment.
3
1 Manual Overview
1.3 Safety Instructions

Device maintenance done in accordance with the following instructions guarantees the safety of both
patients and operators.
 Any failure by hospitals or organizations responsible for using this instrument to implement a
competent repair/maintenance plan is likely to result in abnormal instrument failure or even a
health hazard.
 To prevent risk of explosion, do not use combustible gases (e.g. anesthetics) or liquids (e.g.
ethanol) near this product.
 The machine should be powered off while troubleshooting. Any maintenance operation while the
power is on can lead to electric shock or damage to its electrical components.
 Please connect the device to the socket using a separate fuse and surge protection switch.If the
device shares a fuse and surge protection switch with other equipment, e.g. life support
equipment, any malfunction may cause an electric surge to occur when the instrument is powered
on, which can trip the circuit breaker.
 Maintenance personnel must keep their clothes, hair, and hands away from moving parts such as
the sample probe, jaws, and puncture needle to avoid being stabbed or pinched during
maintenance.
 Parts with special warnings may be subject to mechanical movements and thus lead to injuries
from pinching or stabbing during normal operation or disassembly and repair.
 Operators are obligated to comply with local and national regulations with regard to the disposal
and emission of expired reagents, waste, waste samples, consumables, and so on.
 The reagents are irritating to eyes, skin and mucosa. When handling reagents and their related
items in the laboratory, the operator should comply with laboratory safety regulations and wear
personal protective equipment (such as a protective lab suit, gloves, etc.).
 If the reagent accidentally comes in contact with your skin, wash it off immediately with plenty of
water and see a doctor if necessary.Do the same if you accidentally get any of the reagent in your
eyes.
 Improper maintenance may damage the analyzer. Maintenance personnel should maintain the
device in accordance with the instructions contained in the service manual, and inspect the device
properly after each maintenance.
 If you encounter a problem not specified in the service manual, please contact Dymind's
aftersales service department. A professional will be assigned to offer you maintenance advice.
 When repairing the electronic components of the device, please take off any metal accessories
you might be wearing to prevent personal injury or damage to the device.
 Electrostatic discharge may cause damage to the device’s electronic components. If the repair
process can result in any electrostatic damage, please wear an antistatic wrist strap or maintain
the device on an antistatic workstation.
4
1 Manual Overview
This analyzer must be operated by trained medical testing professionals, doctors, nurses, or
laboratory technicians.
 Samples, controls, calibrators, and waste may pose a potential risk of bio-contamination. When
handling reagents and related items in the laboratory, the operator should comply with laboratory
safety regulations and wear personal protective equipment (such as a protective lab suit, gloves,
etc.).
 Both the parts and surface of the analyzer are potentially infectious, so please take safety
precautions during operation and maintenance.
 The pointed sample probe, any blood stains left on it, QC materials, and calibrators may be
potentially bio-infectious, so please avoid contact with the sample probe.
5
2 Instrument Configuration
2.1 Mechanical Components

The DH36 3-part differential hematology analyzer consists of the main unit and its accessories.
Figure 2-1 Front view of the analyzer
1 - Sample aspiration touch panel 2 - Sample probe
3 - Status indicator light 4 - Thermal printer
5 - Display screen
6
Figure 2-2 Analyzer Profile
1 - LAN network interface 2 - USB interface
3 - Cam lock of the right side door 4 - Sliding door lock of the small left side
door
Figure 2-3 Back view of the analyzer
1 - Power switch 2 - AC input
3 - Cooling fan 4 - Diluent inlet connector
5 - Waste outlet 6 - Waste level detection
7 - Diluent presence detection connector 8 - Ground studs
7
Figure 2-4 Front view of the analyzer (panel cover, rear shell removed)
1 - Sampling assembly 2 - Main control panel
Figure 2-5 Right-side view of the analyzer (right-side door removed)
1 - Power assembly 2 - Sampling assembly
3 - Counting bath assembly 4 - Electromagnetic valve assembly
5 - Negative-pressure chamber 6 - Liquid pump assembly

assembly
8
Figure 2-6 Left-side view of the analyzer (Left-side door removed)
1 - Power assembly 2 - Reagent testing panel
3 - Electromagnetic valve assembly 4 - Sample syringe
2.2 Hydraulics System

The hydraulics system consists of the WBC/HGB measurement module, the RBC/PLT measurement
module, the sampling and blood dispensing module, the power supply and waste discharge module,
and the status monitoring module. Detailed description:
 WBC/HGB measurement module:
Consists of the dosing syringe, WBC/HGB counting bath module, tubing, valve, HGB emission
light, and HGB receiving tube.
 RBC/PLT measurement module:
Consists of the dosing syringe, RBC/PLT counting bath assembly, tubing, and valve.
 Sampling and blood dispensing module:
Consists of the sample probe, sample syringe, swabs, and sampling assembly.
 Power supply and waste discharge module:
Consists of the vacuum chamber, liquid pump, valve, and tubing.
 Status monitoring module:
Consists of the optocoupler and sensor.
9
2.2.1 Schematic Diagram of the Hydraulic System

Figure 2-7 Schematic diagram of the hydraulic system
2.2.2 Hydraulics Diagram for the Overall Unit

Figure 2-8 Hydraulics diagram for the overall unit
10
2.2.3 WBC/HGB Measurement Channel
Channel Principles and a Description of Their Functions

 Measurement principles: Impedance method and colorimetric method
 Measurement parameters: WBC and HGB
 Graph information: WBC histogram
 Reagents for use
 LYE-1: Lyses the RBC and combines with hemoglobin.
 Diluent: This diluting and cleaning agent can provide an electrically conductive environment
and process cells and so on in bulk.
 Function description
 The impedance method is used during WBC measurement: After the addition of LYE-1 lyse,
the red blood cells are lysed. Through the negative-pressure chamber (with a negative
pressure of -30Kpa), the sample is aspirated out of the WBC bath through the aperture. The
WBC particles generate electric pulses when traveling through the aperture, allowing the WBC
cells to be counted according to the number of pulses emitted.
 HGB is measured by colorimetry: Prior to the addition of the blood sample, the baseline
voltage of the diluent is first measured. Then the blood sample and lyse are mixed well for a
complete reaction so that the parameter voltage of the sample can be measured. HGB can
then be calculated based on the local voltage and parameter voltage according to
Lambert-Beer's Law.
Measurement Process
The measurement process for the WBC/HGB measurement module is as follows:
1. Dosing: The diluent syringe is first applied to add the diluent into the WBC bath, and then the
sample probe is used to add the blood sample into the WBC bath, where they are mixed evenly.
After aspirating the diluted sample for the first time, LYE-1 lyse is added into the WBC bath for
incubation.
2. Mixing: Open Valve LV10 to generate air bubbles through the intermittent valve opening to mix the
sample well.
3. Measurement: Open Valve LV07 to aspirate the sample out of the WBC bath through the aperture
by means of the negative-pressure chamber (with a negative pressure of -30Kpa). The WBC
particles generate electric pulses when traveling through the aperture, allowing the WBC cells to
be counted according to the number of pulses emitted.
4. Cleaning: To clean, open LV02 and LV06, and add the diluent into the WBC bath using the diluent
syringe.
5. Waste discharge: Waste is discharged by opening Valve LV09, LV10 and Pump P1.
2.2.4 RBC/PLT Measurement Module
Channel Principles and a Description of Their Functions

 Measurement principles: Impedance method
 Measurement parameters: RBC and PLT
11
 Graph information: RBC histogram and PLT histogram

 Reagents for use
Diluent: This diluting and cleaning agent can provide an electrically conductive environment and
process cells and so on in bulk.
 Function description
RBC and PLT measurement makes use of the impedance method: After the addition of LYA-1 lyse,
the red blood cells are dissolved. Through the negative-pressure chamber (with a negative
pressure of -30Kpa), the sample is aspirated out of the WBC bath through the aperture. The RBC
and PLT particles generate electric pulses of various amplitudes when traveling through the
aperture, allowing them to be counted according to the number of pulses emitted.
Measurement Process
The measurement process for the RBC/PLT measurement module is as follows:
1. Dosing: The diluent syringe is first applied to add the diluent into the RBC/PLT bath as the base
liquid, before using the sample probe to aspirate the sample diluted for the first time from the WBC
bath into the RBC/PLT bath.
2. Mixing: Open Valve LV11 to generate air bubbles through the intermittent valve opening to mix the
sample well.
3. Measurement: Open Valve LV07 to aspirate the sample out of the RBC/PLT bath through the
aperture by means of the negative-pressure chamber (with a negative pressure of -30Kpa). The
RBC and PLT particles generate electric pulses of various amplitudes when traveling through the
aperture, allowing the RBC and PLT cells to be counted according to the number of pulses
emitted.
4. Cleaning: To clean, open Valve LV02 and add the diluent into the RBC/PLT bath using the diluent
syringe.
5. Waste discharge: Waste is discharged by opening Valve LV09, LV11 and Pump P1.
2.2.5 Sampling and Blood-dispensing Module

This module consists of the sample probe, the sample injection tube, the sampling tube, the swab, the
horizontal motor, and the vertical motor.
This is how the sampling and blood-dispensing module works:
Open LV04 and aspirate the sample from the testing tube using the sample syringe→Clean the outer
wall of the sample probe→ …until it reaches the WBC bath→Put aside a fixed amount of blood in the
WBC bath→Clean the outer and inner wall of the sample probe→Aspirate out of the WBC bath a fixed
amount of the sample that has been diluted once→Clean the outer wall of the sample probe and move
the sample probe to the RBC bath→Add into the RBC bath the sample that has been diluted
once→Clean the outer wall of the sample probe to complete the entire process of sampling and blood
dispensing.
In summary, this module works by aspirating the sample and dispensing a fixed amount of the sample
into the corresponding reaction baths, while cleaning the inner and outer walls of the sample probe to
prevent cross-contamination.
12
2.2.6 Power Supply and Waste Discharge Module

 Power supply module
By opening LV09 and Pump P1 to establish a negative pressure of -30Kpa, this module provides
propulsion for the counting of the impedance channel.
 Waste disposal module
This module consists of the following five parts:
 Discharge of waste resulting from swab cleansing
LV08 and Pump 09 are opened to discharge any waste resulting from using the swab to clean
the sample probe.
 Discharge of waste from the WBC bath
Valve LV09, LV10 and Pump P1 are opened to discharge any waste from the RBC/PLT bath.
 Discharge of waste from the RBC/PLT bath
Valve LV09, LV11 and Pump P1 are opened to discharge any waste from the RBC/PLT bath.
 Discharge of waste from the impedance counting bath
After any waste from impedance counting (WBC counting and RBC/PLT counting) flows
through the aperture, and after any waste that results from cleaning the rear section of the
WBC and RBC/PLT baths flows into the negative-pressure chamber, Valve LV09 and Pump
P1 are opened for waste discharge.
2.2.7 Status Monitoring Module

This module involves:
 Monitoring of the diluent
Detection is done by way of a floating sensor; the float moves downwards the diluent level falls,
and an alarm is activated when it reaches empty.
 Monitoring of LYE-1
An optocoupler is used to monitor the LYE-1 level; as it gets lower, the optocoupler’s status
changes until an alarm is activated once there is no LYE-1 left.
 Monitoring of waste overflow
Waste overflow is done by way of a floating sensor; as waste levels rise, the float moves upward
until it eventually activates an alarm indicating that it is full.
13
2.2.8 Hydraulic Components
List of Hydraulic Components
No. Componen Illustration Material No. Function Description Remarks

t No.
1 Sample 56.01.0163A Collects blood samples in the None

probe testing tube and dispenses a
certain portion of the sample to
be placed in the corresponding
counting bath
2 Swab 56.01.0063A Cleans the outer and inner None

walls of the sample probe
3 Two-way 24.13.0010A On-off switch for fluid control None

valve (L)
4 Three-way 24.13.0009A Directional hydraulic valve None

valve (L)
5 Two-way 24.13.0002A On-off switch for fluid control None

valve (S)
6 Three-way 24.13.0003A Directional hydraulic valve None

valve (S)
7 Liquid 24.01.0001A Discharges waste and creates None

pump a vacuum
8 Sample 20.01.0070A Quantitatively divide the blood, None

syringe-AS and add with diluent and lyse
P
14
No. Componen Illustration Material No. Function Description Remarks

t No.
9 Isolation 60.01.0012A This chamber insulates the None

chamber liquid from the electric circuits
to prevent any electrical
interference.
10 WBC/HGB 20.01.0007A 1. This consists of the front None

bath bath and the rear bath, with an
aperture in between; the
sample flows from the front
bath through the aperture to
generate electric pulses that
facilitate WBC counting;
2. The counting bath is
accompanied by a HGB
emission light and a HGB
signal receiving device for
HGB measurement.
11 RBC/PLT 20.01.0008A This consists of the front bath None

bath and the rear bath, with an
aperture in between; the
sample flows from the front
bath through the aperture to
generate electric pulses that
facilitate RBC and PLT
counting.
12 Negative-pr 20.01.0004A 1. This chamber builds the None

essure negative pressure needed to
chamber drive impedance-channel
counting;
2.This chamber builds positive
pressure needed to drive the
mixing;
3. This chamber builds
negative pressure needed to
flush the rear bath of the
impedance counting bath and
to discharge the waste liquid in
the counting bath.
15
List of Tube Types
No. Tube Illustration Material Inner Outer Features

Name No. Diameter Diameter
(mm) (mm)
1 Double-La 63.01.0006 2.0 3.5 Double-layered with

yered A an erosion-resistant
Tube inner layer
2 EVA Tube 63.01.0007 1.0 3 Has good flexibility

A and resistance to
bending and fatigue
3 Thin 50 63.01.0008 1.6 3.2 Soft Tube

Tube A
4 Thick 50 63.01.0009 2.4 4 Soft Tube

Tube A
5 3603 Tube 63.01.0010 3.2 6.4 A soft tube used for

A the external inflow of
diluent and waste
discharge
6 MPF Tube 63.01.0011 2 3.5 A hard tube, resistant

A to pressure and
deformation
7 1.5mm 63.01.0013 1.5 2.5 This tube is hard and

Teflon A transparent, and is
Tube very resistant to
erosion
8 Thick 63.01.0004 1.6 4.8 This tube is soft and

1.6mm A can be connected to
(i.d.) hard tubes
Silicone
Tube
9 3.2mm 63.01.0005 3.2 6.4 This tube is soft and

(i.d.) A can be connected to
Silicone hard tubes; it can also
Tube be used as a
protective shell
10 TPU Tube 63.01.0016 2.4 4.8 A hard tube, resistant

A to pressure and
deformation
16
No. Tube Illustration Material Inner Outer Features

Name No. Diameter Diameter
(mm) (mm)
11 2.0mm 63.01.0020 2 4 This tube is hard, has

(i.d.) A good resistance to
Teflon erosion, and can be
Tube used as an aspiration
tube inside the bottle
of lyse
List of Connector Types
No. Connector Illustration Material Material Features

Name No.
1 T420-1 53.05.0003A White nylon T-type Connector for

Connector 1.6-2.4mm (i.d.) Tubing
2 K420-6005 53.05.0005A PP 10-32 screw-thread

Connector connector for 1.6-2.4mm
(i.d.) tubing
3 S220-6005 53.05.0008A PP, natural 1/4-28 screw-thread

Connector color connector for 1.6-2.4mm
(i.d.) tubing
4 L420-1 53.05.0010A White nylon L-type Connector for

5 Y230-1 53.05.0011A White nylon Y-type Connector for

6 Y420-1 53.05.0012A White nylon Y-type Connector for

7 N430/420-1 53.05.0013A White nylon Thick-to-thin tube

Connector connector for 1.6-2.4mm
configuration
8 N420-6005 53.05.0014A PP, natural Barrel connector for

Connector color 1.6-2.4mm (i.d.) tubing
17
No. Connector Illustration Material Material Features

Name No.
9 MTLL230-1In 53.05.0016A White nylon Integrated locking ring

tegrated
locking ring
10 MTLL013-3 53.05.0044A Red nylon Integrated locking ring

Integrated
locking ring
11 LNS-1 53.05.0023A White nylon Locking bolt for white

Locking bolt panel installation
for panel
installation
12 LNS-3 53.05.0025A Red nylon Locking bolt for red panel

Locking bolt installation
for panel
installation
13 CCLR-1 53.05.0028A White nylon White-coded locking ring

Color-coded
locking ring
14 CCLR-3 53.05.0030A Red nylon Red-coded locking ring

Color-coded
locking ring
2.2.9 Main Measurement Modes

There are two measurement modes including whole-blood mode and predilute mode.
2.2.9.1 Dilution Procedure in Whole-blood Mode

Dilution procedure in whole-blood mode:
18
Figure 2-9 Dilution Procedure in Whole-blood Mode
2.2.9.2 Dilution Procedure in Predilute Mode

Dilution procedure in predilute mode:
Figure 2-10 Dilution Procedure in Predilute Mode
19
2.2.9.3 Measurement Procedure in Whole-blood Mode

In whole-blood mode, a total of 5 time slots for sample measurement are set at 0~4s, 5~17s, 18~30s,
31~44s, and 45~60s, respectively, during each of which different steps are taken. Detailed
description:
 Steps taken between 0~4s (Figure 2-11)
a. Aspirate the whole blood using the sample syringe.
b. Set the sample probe in the upper position for outer-wall cleaning.
Figure 2-11 Measurement Flowchart A
Hydraulics Diagram
T14 T11
T10
T9
T7 LV1 T8 T6 J1
T16 T15 J3
T12
T9 T9 T13
J2
LV6 LV5 LV2 T5
T17 T3
T18 negative-
pressure sensor
T19
T52 T52
T9 T20
J10 T4
J3
T1
T34 T35 J4 T24 T9 T41
T27 T22 T9 T2
T9 T9 T9
T36 J5 T25 J2 J5
J5 T25
T26
T24 LV4 LV3 T9 Diluent
J7 WBC RBC J2 J3
T24 T24 T23
J6 J6 T21 J9
T28 T25 J6 T50
J6 T25 T49
T9 T9 J6 T48 J8
T29 T30 T9 T29 T30 T42 T47
T9 T9
T37 T53
T31 LV7 J4
T53 LV8 T43 LV9 T46
T53 T51
J4 T32 T31 T33 J8 J8
syringe
T54 J11 T54 J11 T45
T44
LV10 LV11
T39
T33
T40 P1 waste
ECN No. Rev Design Check Approval Date
3-way valve 2-way valve

3 1 2:COM 1
1:NC 2:IN
3:NO 2 1:OUT
2
Note：LV-Liquid valve； P-pump；T-pipe； J-connector

a. Insert the sample probe into the WBC/HGB bath.
b. The contaminated sample is removed from the tip of the sample probe from inserting it into the
WBC bath.
c. Drain the blood sample and diluent from the WBC/HGB bath.
d. Add the diluent into the WBC/HGB bath.
e. Add the blood sample into the WBC/HGB bath and mix it through with air bubbles.
f. Drain the RBC/PLT bath.
g. Add the base solution into the RBC/PLT bath.
20
Figure 2-12 Measurement Flowchart B

a. Move the sample probe to the upper position and clean its inner and outer walls.
b. Insert the sample probe into the WBC/HGB bath to aspirate the diluted sample.
c. Move the sample probe to the upper position and above the RBC/PLT bath, and then into the
RBC/PLT bath.
d. Add the diluted sample into the RBC/PLT bath once with the sample probe, and mix well using
the air bubbles.
e. Add the LYE-1 lyse into the WBC/HGB bath and mix through with air bubbles.
f. Move the sample probe upward into the upper position and clean its outer wall.
g. A pressure of -30Kpa is created in the negative-pressure chamber.
21
Figure 2-13 Measurement Flowchart C

Hydraulics Diagram
T14 T11
T10
T9
T7 LV1 T8 T6 J1
T16 T15 J3
T12
T9 T9 T13
J2
LV6 LV5 LV2 T5
T17 T3
T18 negative-
pressure sensor
T19
T52 T52
T9 T20
J10 T4
J3
T1
T34 T35 J4 T24 T9 T41
T27 T22 T9 T2
T9 T9 T9
T36 J5 T25 J2 J5
J5 T25
T26
J7 WBC RBC J2 J3
T24 T24 T23
J6 J6 T21 J9
T28 T25 J6 T50
J6 T25 T49
T9 T9 J6 T48 J8
T29 T30 T9 T29 T30 T42 T47
T9 T9
T37 T53
T31 LV7 J4
T53 LV8 T43 LV9 T46
T53 T51
J4 T32 T31 T33 J8 J8
syringe
T54 J11 T54 J11 T45
T44
LV10 LV11
T39
T33
T40 P1 waste

3 1 2:COM 1
1:NC 2:IN
3:NO 2 1:OUT
2
 Steps taken between 31~44s

Data of WBC channel and RBC channel is under measurement.
a. Measurement of WBC channel and RBC channel has completed.
b. Drain the WBC/HGB bath.
c. Add the diluent into the WBC/HGB bath for cleansing.
d. Zap the WBC/HGB bath.
e. Zap the RBC/PLT bath.
f. Drain the WBC/HGB bath before adding the diluent as the base solution.
g. Drain the RBC/PLT bath before adding the diluent as the base solution.
h. Clean the rear baths of the WBC/HGB and RBC/PLT baths.
i. Restore the sample assembly to measurement preparation status.
j. At around the 56s mark, the screen will show the current counting results.
k. At around the 60s mark, the sample probe is set to the blood-sampling position, which
indicates that it is ready to take the next sample.
22
Figure 2-14 Measurement Flowchart D

Hydraulics Diagram
T14 T11
T10
T9
T7 LV1 T8 T6 J1
T16 T15 J3
T12
T9 T9 T13
J2
LV6 LV5 LV2 T5
T17 T3
T18 negative-
pressure sensor
T19
T52 T52
T9 T20
J10 T4
J3
T1
T34 T35 J4 T24 T9 T41
T27 T22 T9 T2
T9 T9 T9
T36 J5 T25 J2 J5
J5 T25
T26
J7 WBC RBC J2 J3
T24 T24 T23
J6 J6 T21 J9
T28 T25 J6 T50
J6 T25 T49
T9 T9 J6 T48 J8
T29 T30 T9 T29 T30 T42 T47
T9 T9
T37 T53
T31 LV7 J4
T53 LV8 T43 LV9 T46
T53 T51
J4 T32 T31 T33 J8 J8
syringe
T54 J11 T54 J11 T45
T44
LV10 LV11
T39
T33
T40 P1 waste

3 1 2:COM 1
1:NC 2:IN
3:NO 2 1:OUT
2
2.2.9.4 Measurement Procedure in Predilute Mode

The measurement principle in predilute mode is generally the same with that in whole-blood mode,
while, the difference lies in that the whole-blood measured shall be quantitatively prediluted prior to
the measurement in predilute mode.
2.2.10 Hydraulics Maintenance
2.2.10.1 Swab Cleaning and Maintenance

In the case of serious device contamination, our service team can provide on-site service either
regularly or by request. The swab requires cleaning and maintenance to get rid of any contamination
found on its lower surface.
The operating procedure as follows: Shut down the device and disassemble the swab. A certain
amount of probe cleanser is diluted with the diluent into a 1:3 (probe cleanser to diluent) solution.
Repeatedly scrub the bottom end of the swab and the inside of its lower aperture with a Q-tip
dampened with the diluted probe cleanser. After scrubbing, use another clean Q-tip dampened with
pure diluent to clean away the probe cleanser residue at the bottom end of the swab and the inside of
its lower aperture. See Figure 2-15.
23
Figure 2-15 Cleaning the swab
After cleaning and reassembling the swab (and ensuring that the inflow/outflow interface tubing is
connected correctly), turn on the device and go to Service > Clean. Click the Clean Sample Probe
icon to perform the operation before performing cleaning and maintenance on the swab.
2.2.10.2 Cleaning and Maintenance of the WBC Bath

Log into the analyzer software interface and go to Service > Maintenance > Clean. Click Clean WBC
Bath. See Figure 2-16.
Figure 2-16 Cleaning and maintenance interface
2.2.10.3 Cleaning and Maintenance of the RBC Bath

Log into the analyzer software interface and go to Service > Maintenance > Clean. Click Clean RBC
Bath.
2.2.11 Troubleshooting Common Hydraulics Problems
2.2.11.1 Commonly Used Equipment and Tools
Name Illustration Usage Remarks
1. Used to contain liquid after a tube is

pulled out.
Small sample
2. Used as a diluent container. None
cup
3. The ratio of probe cleanser to diluent is
1:3.
24
Name Illustration Usage Remarks
plastic syringe 1. Used to manually unclog the WBC and

(disposal syringe RBC channels; None
with no pillow) 2. Used to inject other liquids.
Barrel connector tubing connection None
Used to connect tubing and the plastic

Silicone tube None
syringe
1. Used to scrub and clean the swab.

Q-tips 2. Used for other scrubbing and cleaning None
tasks.
2.2.11.2 Inspection and Troubleshooting of Valve Clogging

To prevent the possibility of liquid flowing onto the base panel when the tube connected to the valve is
pulled out, the tube pulled out needs to be placed into the small sample cup, and tissue paper should
be used as a pad to prop up any affected components. The sample cup should be removed once the
liquid has stopped flowing.
Disassemble the valve. Open the valve cap to identify any clogging caused by impurities After the
impurities have been removed, place the components in their original positions (note that the parts
with instructions are kept on the same side during installation, as shown in Figure 2-17~Figure 2-21.
Then remove the tissue paper and scrub and clean the bottom.)
Figure 2-17 3-way valve (L) Figure 2-18 2-way valve (L)
25
Figure 2-19 Installation of the large valve
Figure 2-20 Installation of the 3-way Figure 2-21 Installation of the 2-way valve (S)
valve (S)
2.2.11.3 Liquid Pump Clogging: Inspection and Troubleshooting

1. Pull out the tubing and place it into the small sample cup. Then prop up any relevant parts with
tissue paper and disassemble the pump. Use the Phillips screwdriver to open the pump cap to
observe any clogging resulting from impurities, as shown in Figure 2-22.
Figure 2-22 Pump cap
2. Remove the impurities and place the components in their original positions. Make sure to align the
two marked lines correctly while installing (as shown in Figure 2-23).
26
Figure 2-23 Installing the marked lines
aligned
3. When reassembling the pump, make sure the liquid inlet and outlet are connected to the correct
tubing (e.g. Figure 2-24).
Figure 2-24 Symbols for inflow and outflow direction
2.2.11.4 Aperture Clogging of the WBC Channel

Aperture clogging can be resolved by implementing the procedure for cleaning the WBC bath and
using the aperture backflush.
1. Go to the Service > Maintenance > Clean screen in the analyzer software interface, and click the
WBC bath button.
2. Go to the Service > Maintenance > Maintain screen, and click the Flush Apertures and then the
zap Apertures button.
3. After you are done, click the Remove Error button to check if the aperture has been unclogged.
If clogging persists, the probe cleanser needs to be manually pushed through to clean the
WBC-channel aperture, and the following steps should be taken:
1. After obtaining permission from qualified service personnel, first drain the WBC bath (the liquid
can also be aspirated dry manually using a plastic syringe or other tools): Go to the Service >
Maintenance > Maintain screen, click the Empty WBC bath button (note that a prompt box will
pop up for confirmation after draining; do not click the OK button, otherwise the WBC bath will be
refilled with liquid).
2. Pull out the tubing connected to the WBC outflow tube and connect the plastic syringe filled with
the diluted probe cleanser (with a ratio of probe cleanser to diluent of 1:3) to the WBC outflow tube
(as shown in Figure 2-25). Pushing the plastic syringe back and forth will facilitate the repeated
27
flushing of the aperture with the probe cleanser. Apply proper force while pushing to prevent the
tube from dropping and spilling (you can hold the tube with your hand to keep it in place).
Figure 2-25 Cleaning the WBC-bath aperture
3. Flush back and forth ten times, and then let it settle for 3 minutes. Afterward, flush it three more
times with a syringe containing the pure diluent using the aforementioned method.
4. After the diluent cleaning, drain the liquid inside the WBC bath and reconnect the original tubing.
To drain the WBC bath by interface operation, click the OK button in the prompt confirmation box
after draining, and then click the Remove Error button to see if the clogging problem is resolved.
5. If aperture clogging persists, redo the above procedures until the problem has been resolved.
2.2.11.5 Aperture Clogging of the RBC Channel

The solution is identical to 2.2.11.4 Aperture Clogging of the WBC Channel, except that the target
object is the RBC bath.
2.2.11.6 Overflowing of the WBC Bath

First wipe off the liquid with tissue paper, and perform the following troubleshooting steps.
1. Check if Pump1 is clogged.
Follow the steps specified in 2.2.11.3 Liquid Pump Clogging: Inspection and Troubleshooting
to inspect and resolve any clogging of the liquid pump. After troubleshooting, run the counting
procedure to see if the problem has been resolved.
If the problem has been resolved, end the procedure; if the problem persists, continue to perform
the following steps:
2. Check if Valve 10 is clogged.
28
Follow the steps specified in 2.2.11.2 Inspection and Troubleshooting of Valve Clogging to
inspect and resolve any clogging of Valve 10. After the troubleshooting, run the whole-blood
counting procedures to see if the problem has been resolved.
3. Check if there are any kinks or damage along the tubing.
Check if there are any kinks or damage along the tubing, as highlighted in red in Figure 2-26, and
replace the corresponding tubing if the problem persists. After the troubleshooting, run the
whole-blood counting to see if the problem has been resolved.
Figure 2-26 WBC tubing for fluid discharge
Hydraulics Diagram
T14 T11
T10
T9
T7 LV1 T8 T6 J1
T16 T15 J3
T12
T9 T9 T13
J2
LV6 LV5 LV2 T5
T17 T3
T18 negative-
pressure sensor
T19
T52 T52
T9 T20
J10 T4
J3
T1
T34 T35 J4 T24 T9 T41
T27 T22 T9 T2
T9 T9 T9
T36 J5 T25 J2 J5
J5 T25
T26
J7 WBC RBC J2 J3
T24 T24 T23
J6 J6 T21 J9
T28 T25 J6 T50
J6 T25 T49
T9 T9 J6 T48 J8
T29 T30 T9 T29 T30 T42 T47
T9 T9
T37 T53
T31 LV7 J4
T53 LV8 T43 LV9 T46
T53 T51
J4 T32 T31 T33 J8 J8
syringe
T54 J11 T54 J11 T45
T44
LV10 LV11
T39
T33
T40 P1 waste

3 1 2:COM 1
1:NC 2:IN
3:NO 2 1:OUT
2
4. Check whether or not the isolation chamber is airtight.
After obtaining permission from qualified service personnel, first drain the WBC bath (the liquid
can also be aspirated dry manually using a plastic syringe or other tools): Go to the Service >
Maintenance > Maintain screen, click the Empty WBC bath button (note that a prompt box will
pop up for confirmation after draining; do not click the OK button, otherwise the WBC bath will be
refilled with liquid).
Disassemble the isolation chamber below the WBC bath and pull out the corresponding tubing.
Use the thick No. 50 tube or 1.6mm (i.d.) silicone tube to attach the two connectors to the side of
the isolation chamber, plugging both apertures. Then attach the plastic syringe to the connector
above the isolation chamber using a 1.6mm (i.d.) silicone tube. Push the plastic syringe to
pressurize the isolation chamber, and hold the syringe steady for 30 seconds to check for any
appearance of air bubbles. If any air bubbles occur, the isolation chamber needs to be replaced.
See the picture below
29
Figure 2-27 Checking whether or not the isolation chamber is airtight
5. After inspection, the components and tubing need to be restored to their original locations. Click
the OK button in the confirmation box that pops up after the counting bath is drained by opening
the user interface.
If the liquid level in the WBC bath is found to be too high, immediately shut down the device to prevent
any further overflowing.
2.2.11.7 Overflowing of the RBC Bath

The troubleshooting procedure is the same as that in 2.2.11.6 Overflowing of the WBC Bath; the
only difference is that valve to be inspected is Valve 11. The tubing for inspection is shown in Figure
2-28, and the isolation chamber to be inspected is the one below the RBC bath.
Figure 2-28 RBC tubing of liquid discharge
30
2.2.11.8 Leakage from the Swab

First wipe off any liquid with tissue paper, and then perform the following troubleshooting steps:
1. Check if Pump 1 is clogged.
Follow the steps specified in 2.2.11.3 Liquid Pump Clogging: Inspection and Troubleshooting
to inspect and resolve any clogging of the liquid pump. After the troubleshooting, run the
whole-blood counting procedures to see if the problem has been resolved.
2. Check if Valve 8 is working normally.
Follow the steps specified in 2.2.11.2 Inspection and Troubleshooting of Valve Clogging to
inspect and resolve any clogging of Valve 8. After the troubleshooting, run the counting procedure
to see if the problem has been resolved.
3. Check if there are any kinks or damage along the tubing.
Check if there are any kinks or damage along the tubing, as highlighted in red in Figure 2-29, and
replace the corresponding tubing if the problem persists. After the troubleshooting, run the
whole-blood counting to see if the problem has been resolved.
Figure 2-29 Swab tubing for fluid aspiration
Hydraulics Diagram
T14 T11
T10
T9
T7 LV1 T8 T6 J1
T16 T15 J3
T12
T9 T9 T13
J2
LV6 LV5 LV2 T5
T17 T3
T18 negative-
pressure sensor
T19
T52 T52
T9 T20
J10 T4
J3
T1
T34 T35 J4 T24 T9 T41
T27 T22 T9 T2
T9 T9 T9
T36 J5 T25 J2 J5
J5 T25
T26
J7 WBC RBC J2 J3
T24 T24 T23
J6 J6 T21 J9
T28 T25 J6 T50
J6 T25 T49
T9 T9 J6 T48 J8
T29 T30 T9 T29 T30 T42 T47
T9 T9
T37 T53
T31 LV7 J4
T53 LV8 T43 LV9 T46
T53 T51
J4 T32 T31 T33 J8 J8
syringe
T54 J11 T54 J11 T45
T44
LV10 LV11
T39
T33
T40 P1 waste

3 1 2:COM 1
1:NC 2:IN
3:NO 2 1:OUT
2
4. Check if there is perforation at the bottom of the sample probe.
Set the small sample cup below the probe. When pulling out the tube above the probe (push the
tube end outward to pull it out; otherwise there will be crimping in the sampling tube connected to
the end of the probe), disassemble the probe, and connect it to the plastic syringe filled with the
diluent using a 1.6mm (i.d.) silicone tube. Manually push the syringe to see if any liquid flows from
31
the bottom end of the probe. If such a flow is detected, this indicates that perforation exists at the
bottom of the probe (the liquid normally flows from the sides. Be sure to collect the waste when
pushing the liquid to the correct location, as shown in Figure 2-30).
Figure 2-30 Check the liquid pushing of the sample probe
Restore the tubing to its original status (cut off the crimped part at the front end of the sampling
tube, and readjust the tubing for installation. Make sure to check if the sampling tubing gets into
the way of other assemblies whenever the sampling assembly moves up and down, or if it feels
too tight. If this is the case, continue to adjust the sampling tubing until it looks like it fits properly.)
5. Check for any wear and tear in the top aperture of the swab.
To disassemble, take off the swab's fixed jump ring. Pull out the swab's inflow and outflow tubes.
Measure the upper-section aperture with a caliper; an aperture with a diameter of more than
1.71mm indicates that the swab needs to be replaced.
When reinstalling or replacing the swab, first put the inflow tube and outflow tube in place (Note:
The inflow tube is the Thin 50 tube, connected to the bottom connector; the outflow tube is the
Thick 50 tube, connected to the top connector), then put on the swab (insert the probe into the
swab aperture) and tap the swab's fixed jump ring to complete the procedure.
2.2.11.9 Problems with Creating Positive Pressure

The troubleshooting steps are:
1. Check if the pressure chamber is broken: Disassemble the pressure chamber if necessary and
seal the openings with rubber tubes. Then place the pressure chamber into the water tank. Use a
syringe or other equipment to pressurize the pressure chamber. Any air bubbles detected indicate
that the pressure chamber needs to be replaced.
2. Check if there are any kinks or damage along the tubing to the pressure chamber. Any
replacements should be made using tubing of the proper length and type.
32
Figure 2-31 Tubing of the positive-pressure chamber

Hydraulics Diagram
T14 T11
T10
T9
T7 LV1 T8 T6 J1
T16 T15 J3
T12
T9 T9 T13
J2
LV6 LV5 LV2 T5
T17 T3
T18 negative-
pressure sensor
T19
T52 T52
T9 T20
J10 T4
J3
T1
T34 T35 J4 T24 T9 T41
T27 T22 T9 T2
T9 T9 T9
T36 J5 T25 J2 J5
J5 T25
T26
J7 WBC RBC J2 J3
T24 T24 T23
J6 J6 T21 J9
T28 T25 J6 T50
J6 T25 T49
T9 T9 J6 T48 J8
T29 T30 T9 T29 T30 T42 T47
T9 T9
T37 T53
T31 LV7 J4
T53 LV8 T43 LV9 T46
T53 T51
J4 T32 T31 T33 J8 J8
syringe
T54 J11 T54 J11 T45
T44
LV10 LV11
T39
T33
T40 P1 waste

3 1 2:COM 1
1:NC 2:IN
3:NO 2 1:OUT
2
3. Check if the pump is working; if not, replace the pump.

4. Check if the positive-pressure sensor and the corresponding electrical circuit are in normal
working order. First replace a reagent testing panel and reconnect its tubing, then check if the
positive pressure is normal in the status interface; if it is normal after the replacement, this means
that the positive-pressure sensor and the corresponding electrical circuit are causing a problem
with building positive pressure (After the reagent testing panel is replaced, note that the tubing
needs to be connected correctly. First keep a record of where it was by marking it before
disassembling the tubing).
5. Check the valve directly connected to the tubing of the positive-pressure chamber to see 1)
whether the on-off switch is normally; and 2) whether there is any clogging.
6. After troubleshooting, complete the steps shown in the Remove Error wizard.
2.2.11.10 Problems in Creating the Negative Pressure

The troubleshooting steps are:
1. Check if the negative-pressure chamber is broken: Disassemble the negative-pressure chamber if
necessary and seal the openings with rubber tubes. Then put the negative-pressure chamber into
the water tank. Use a syringe or other equipment to pressurize the negative-pressure chamber.
Any air bubbles that occur indicate that the negative-pressure chamber needs to be replaced.
2. Check if there are any kinks or damage along the tubing connected to the negative-pressure
chamber. Any replacements should be made using tubing of the proper length and type.
33
Figure 2-32 Tubing of the negative-pressure chamber

Hydraulics Diagram
T14 T11
T10
T9
T7 LV1 T8 T6 J1
T16 T15 J3
T12
T9 T9 T13
J2
LV6 LV5 LV2 T5
T17 T3
T18 negative-
pressure sensor
T19
T52 T52
T9 T20
J10 T4
J3
T1
T34 T35 J4 T24 T9 T41
T27 T22 T9 T2
T9 T9 T9
T36 J5 T25J2 J5
J5 T25
T26
J7 WBC RBC J2 J3
T24 T24 T23
J6 J6 T21 J9
T28 T25 J6 T50
J6 T25 T49
T9 T9 J6 T48 J8
T29 T30 T9 T29 T30 T42 T47
T9 T9
T37 T53
T31 LV7 J4
T53 LV8 T43 LV9 T46
T53 T51
J4 T32 T31 T33 J8 J8
syringe
T54 J11 T54 J11 T45
T44
LV10 LV11
T39
T33
T40 P1 waste

3 1 2:COM 1
1:NC 2:IN
3:NO 2 1:OUT
2
3. Check if Liquid Pump 2 is working; if not, replace the pump.

4. Check if the negative pressure sensor and the corresponding electrical circuit are in normal
working order. First replace a reagent testing panel and reconnect its tubing; then check if the
negative pressure is normal in status interface. If it is normal after the replacement, this means
that the negative-pressure sensor and the corresponding electrical circuit have caused a problem
with building negative pressure (After the reagent testing panel is replaced, note that the tubing
needs to be connected correctly. First keep a record of where it was by marking it before
disassembling the tubing).
5. Check the valve directly connected to tubing of the negative-pressure chamber to see 1) whether
the on-off switch is normally; and 2) whether there is any clogging.
6. After troubleshooting, complete the steps shown in the Remove Error wizard.
2.3 Hardware System

The hardware system of the Auto Hematology Analyzer consists of the main control panel, reagent
testing panel, front panel, reagent sealing panel, etc. and its block diagram is shown in Figure 2-33.
34
Figure 2-33 Block Diagram of Hardware System
Display screen Front panel (LED,

driver panel buzzer)
Reagent Reagent
Closing Panel Main Control Panel Testing Panel
Other external connecting unit

(valve, pump, motor, sensor)
Hardware maintenance principles apply to all the panels. All the panels have passed hardware tests
before leaving the factory, and any hardware problem found lcater may be mainly caused by power
problems. Thus, hardware maintenance primarily involves the power supply. Hardware problems and
maintenance methods for each panel are described in the subsequent sections.
2.3.1 Main Control Panel

The main control panel is the core panel of the DH hematology analyzer; the device cannot work
properly with a problematic main control panel. This section introduces the main control panel from
the perspectives of composition, problem identification, and maintenance.
35
2.3.1.1 Composition of the Main Control Panel

Figure 2-34 Block Diagram of Main Control Panel Functions
monitoring
Impedance
channel
Other
External interface
Constant current
Burning control
source control
HGB control
Driver control
module
The block diagram above gives a brief overview of the composition of main control panel and the
function of each module. Further description can be found below based on the pictures for each panel.
Picture of main control panel is shown in Figure 2-35.
36
Figure 2-35 Main control panel

1
8
3
7
4
5 6
1 - External wiring interface 2 - Network interface and USB interface
3 - Digital circuit module 4 - Debugging interface
5 - Motor, pump valve and 6 - Analog impedance channel module

temperature-control module
7 - Voltage-multiplying circuit module 8 - Analog HGB channel module
The main control panel can be clearly divided into digital part, analog part and driver part. Among
them:
 The left half of the panel is the digital part, including ARM unit, FPGA unit, external interface,
debugging interface, power supply, etc.
 The right half of the panel is the analog part, including impedance channel, hardware monitoring
channel, external interface, power supply, etc.
 The lower left corner of the panel is the driver part, including motor driver, valve & pump driver,
temperature control, etc.
 In the middle of the panel there are AD and interface chips, where AD is responsible for
analog-to-digital conversion and the interface chip is responsible for on-off control for relevant
functions of the analog part.
37
2.3.1.2 Peripheral Interface of the Main Control Panel

There are several interfaces on the main control panel, and the detailed interface description is shown
in Table 2-1.
Table 2-1 Definition Table of External Interfaces on the Main Control Panel
No. Tag No. Description
1 J2 RBC analog signal interface
2 J21 Motor driver interface
3 J22 Motor driver interface
4 J23 Valve driver interface
5 J24 Valve driver interface
6 J17 Power supply input interface
7 J37 Pump driver interface
8 J39 Heating rod control interface
9 J40 Heating rod control interface
10 J7 MPU_JTAG interface
11 J12 FPGA_JTAG interface
12 J13 FPGA_AS interface
13 J31 Pressure sensor signal interface
14 J35 Front display panel interface
15 J43 Network interface
16 J46 USB interface
17 J47 USB interface
18 J14 Back-light panel interface
19 J44 Display screen data communication interface
20 J45 Touch screen data interface
21 J4 Serial port
22 J5 Serial port
23 J48 Printer interface
24 J26 Reagent testing signal interface
25 J27 Micro-switch signal interface
26 J28 Motor position optocoupler signal interface
27 J29 Motor position optocoupler signal interface
28 J30 Temperature sensor interface
29 J18 5V power inlet
38
No. Tag No. Description
30 J19 5V power inlet
31 J20 5V power outlet
34 J11 HGB signal interface
35 J10 ±12V power inlet
36 J16 110V burning power input interface
The debugging interfaces designated for the main control panel are serial interface J7 (RS232
signals), FPGA JTAG interface J3, FPGA AS interface J4, and SD interface for programming before
shipping. Use of these debugging interfaces is restricted to developers only.
2.3.1.3 Power input and indicator lights on the Main Control Panel
Digital Power Input and Indicator Lights

Power input of the digital part is 5V, and it can be tested via testing point TP15 (multimeter shall be
adopted for testing, and during the testing, the red probe shall be connected to TP15, while the black
probe shall be connected to the metal part on the housing). The 5V power input is accompanied by the
fuse F2, Normally the fuse will not be blown since the power supply of the device is equipped with
surge protection. If a short circuit occurs under the input of 5V of power, the power supply will fail to
work properly and a buzzing sound can be heard.
All digital power supplies of the main control panel are equipped with an indicator light and testing
point, and corresponding relationships between the digital power supply, indicator light, and testing
point is shown in the table below.
Position Code of Meaning Normal Status

Indicator Light
D21 5V digital power supply On
D17 3.3V digital power supply On
D14 TPS65910 3.3V working status1 On

NOTE:
1- D14 usually indicates the working status of the ARM power management chip, TPS65910A31A1, but
this indicator light only indicates the working status of one LDO signal channel in TPS65910; the
multimeter can be used to test the corresponding testing points for other LDO and DC-DC channels. For
details, see "Digital Testing Points" in the section of 2.3.1.4 Testing Points on the Main Control Panel.
39
Analog Power Input and Indicator Lights

The analog power input of +12V and -12V on the main control panel can be tested for actual voltage
values via the testing points TP21 and TP22. The +12V power input is accompanied by the fuse F4,
while -12V is accompanied by the fuse F5. Like the 5V digital power input, +12V and -12V derive
power from the device's power supply; if both are subject to overload, they will receive surge
protection from the power source and the corresponding indicator lights will not be activated.
The following table lists the analog power indicator lights:
Position Code of Indicator Meaning Normal Status

Light
D18 +12V power indicator light On
D19 -12V power indicator light On
D23 -5V power indicator light On
D25 +5V power indicator light On
+5V and -5V originate from the linear stabilizer. The corresponding lights will not be activated by the
+5V or -5V overload, and the linear stabilizer will be very hot. If either the +5V or the -5V power
indicator light is not on, immediately power off the device and troubleshoot the corresponding
electrical circuits. We recommend replacing the main control panel.
Other Indicator Lights on the Main Control Panel

The ARM and FPGA units are each assigned a single indicator light to show their working status on
the main control panel. In addition, a USB0 power indicator light is designated for the ARM unit.
The following table lists the indicator lights:
Position Code of Meaning Normal Status

Indicator Light
D79 It indicates the ARM working status and flashes Flashing

after proper system loading.
D90 It indicates the FPGA working status and Flashing

flashes after proper system loading.
D52 It indicates the MPU working status and flashes Flashing

after proper system loading.
Indicator lights are very useful in practice; hardware problems can be identified by the status of the
indicator lights. For details, see 2.3.1.5 Identification of Main Control Panel Problems.
2.3.1.4 Testing Points on the Main Control Panel

There are two types of testing points on the main control panel: Digital and analog.
Digital Testing Points

The digital testing points on the main control panel listed here are commonly used ones, in particular
for power supply and key signals:
40
Position Code of Description

Testing Points
TP15 the testing point for 5V digital input, with the expected
voltage of 5V
TP9 TPS65910 VRTC output, with the expected voltage of 1.8V
TP61 DDR3 reference voltage, with the expected value of 0.75V
TP7 TPS65910 VDIG1 output, with the expected value of 1.8V
TP8 TPS65910 VDIG2 output, with the expected value of 1.8V
TP6 TPS65910 VAUX33 output, with the expected value of

3.3V
TP5 TPS65910 VMMC output, with the expected value of 3.3V
TP3 TPS65910 VAUX2 output, with the expected value of 3.3V
TP4 TPS65910 VAUX1 output, with the expected value of 1.8V
TP1 TPS65910 VDAC output, with the expected value of 1.8V
TP2 TPS65910 VPLL output, with the expected value of 1.8V
TP20 the testing point for 3.3V digital power supply, with the
expected voltage of 3.3V
LS_CLK DIFF low-angle AD clock,4MHz clock signal
MS_CLK DIFF medium-angle AD clock, 4MHz clock signal
HS_CLK DIFF high-angle AD clock, 4MHz clock signal
WBC_CLK WBC-channel AD clock, 1MHz clock signal
RBC_CLK RBC-channel AD clock, 1MHz clock signal
Analog Testing Points

The following table lists the analog testing points:
Position Code Description

of Testing
Points
TP5 AVCC_+5V testing point, with the expected voltage of 5V
TP21 AVCC_+12V testing point, with the expected voltage of +12V
41

of Testing
Points
TP22 AVCC_-12V testing point, with the expected voltage of -12V
TP25 AVCC_-5V testing point, with the expected voltage of -5V
TP58 HGB_LED_CTL testing point, HGB light-driven control switch; 0

for on and 1 for off
TP47 WBC channel first-level OPAMP output testing point
TP46 WBC channel third-level OPAMP output testing point
TP43 WBC channel fourth-level OPAMP output testing point
TP44 WBC channel sixth-level OPAMP output testing point
TP45 WBC channel seventh-level OPAMP output testing point
WBC_AD WBC-channel AD front-end testing point
TP39 RBC channel first-level OPAMP output testing point
TP40 RBC channel third-level OPAMP output testing point
TP38 RBC channel fourth-level OPAMP output testing point
TP41 RBC channel sixth-level OPAMP output testing point
TP42 RBC channel eighth-level OPAMP output testing point
RBC_AD RBC-channel AD front-end testing point
TP31 SELECT_WBC_CTL testing point, the switch control signal for

WBC bath zap and CC source; 0 for zap and 1 for CC source
TP29 SELECT_RBC_CTL testing point, the switch control signal for

RBC bath zap and CC source; 0 for zap and 1 for CC source
TP53 VCONST_MON_AD testing point, 1.36V±0.2V
TP28 RH_MON testing point, 1.9V±0.2V
TP30 WH_MON testing point, 1.7±0.2V
TP33 VCONST_CTL testing point, the switch control signal for CC

source; 0 for on and 1 for off
LSIN the testing point for DIFF low-angle signal input
LS_AD DIFF low-angle AD front-end testing point
MSIN the testing point for DIFF medium-angle signal input
MS_AD DIFF medium-angle AD front-end testing point
HSIN the testing point for DIFF high-angle signal input
HS_AD DIFF high-angle AD front-end testing point
HGB_AD HGB-channel AD front-end testing point
42

of Testing
Points
TP36 AVCC_+12VMON_AD testing point, reflecting the voltage of

AVCC_+12V power source
TP35 AVCC_-12VMON_AD testing point, reflecting the voltage of

AVCC_+12V power source
2.3.1.5 Identification of Main Control Panel Problems

Problems with the main control panel can be categorized as power problems, connection problems,
and functional problems. Power problems can be identified using the status of power indicator lights,
and connection problems can identified by way of direct observation; however, functional problems
are a little tricky, and can be indirectly shown using other indicators.
Power Problems of the Main Control Panel and their Indicators

The power supply of the main control panel is clearly divided between digital and analog. Among
them:
 A digital power input of 5V is enabled by the upper middle J18, J19 interfaces on the main control
panel, as shown in Figure 2-36.
Figure 2-36 Digital Power Input, Testing Point and Indicator Light
1 - 5V power inlet 2 - testing point for 5V power supply
3 - 5V power indicator light

 An analog power input of +12V and -12V is enabled by the upper right J10 interface on the main
control panel, as shown in Figure 2-37.
43
Figure 2-37 Analog power input
1 - ±12V power inlet 2 - +12V testing point
3 - -12V testing point

 A power input of 12V and 24V is enabled by the lower left J17 interface on the main control panel,
as shown in the figure below.
1 - 12V and 24V power input interface 2 - 24V testing point
3 - 12V testing point

All power inputs on the main control panel derive from the power input of the device. If either of the
power inputs on the main control panel receives a surge from a grounded or any other input power
source, the device’s power supply will activate its automatic protection mechanism and produce a
buzzing sound.
For the normal status of power indicator lights shown in the figures above, please refer to the
descriptions of the power supply and indicator lights of the main control panel in 2.3.1.1 Composition
of the Main Control Panel. If the power indicator light is in a status different from normal, this means
that the power supply is not working properly.
U11 deserves further description as follows. U11 is the ARM power management chip in the digital
44
section of the main control panel. The working status of this chip determines the working status of the
main control panel. As shown in Figure 2-38, D14 turned on indicates the proper working of U11.
Figure 2-38 ARM power management chip in the main control panel
1 - Power management chip U11 2 - U11 working indicator
Functional Problems with the Main Control Panel and their Indicators
Functional problems with the main control panel are typically related to one of two causes. One cause
may be that the counting function of the main control panel cannot be realized. For example, the
digital part’s operating system on the main control panel is not operational, the FPGA program does
not load, or there is abnormal counting. The other cause refers to control failure of the motor,
valve-pump, temperature control, etc.
 ARM OS on the main control panel fails to boot
The ARM on the main control panel boots from NAND using its default factory setting, so the
booting media selection switch S6 should be turned to NAND (i.e. OFF state for DIP switches), as
shown in Figure 2-39.
Figure 2-39 ARM booting and running
1 - DIP switch status 2 - ARM normal working indicator light D79

When the ARM OS is booted properly, the working-status indicator light D79 will flash on the main
control panel. If D79 is not lit up, it means that the ARM OS has failed to boot.
45
 MPU program loading fails

When the MPU program is booted properly, the working-status indicator light D52 will flash on the
main control panel. If D52 is not lit up, it means that the MPU program has failed to boot. The
location of D52 is shown below.
1 - MPU status indicator light

 Loading problems with the FPGA program
The FPGA program is loaded from the serial port FLASH. A successful loading of the FPGA will
cause the indicator light D90 to flash. If D90 is not lit up, it means that FPGA program has not
loaded. The location of D90 is shown in Figure 2-40.
Figure 2-40 Indicator of FPGA working status
1 - FPGA status indicator light

 Counting abnormalities
There are many causes that lead to counting abnormalities, and they can include problems with
main control panel problem. Counting abnormalities caused by main control panel problems
usually involve counting results of zero or overly high counting results on multiple occasions. A
counting result of zero usually results from a failure to apply the CC source to the counting bath,
while an overly high counting result is usually caused by too much noise in the analog-channel
circuit.
46
2.3.1.6 Maintenance of the Main Control Panel

The maintenance of the main control panel mostly deals with problems not related to connections. In
theory, only designated maintenance professionals are allowed to perform the maintenance steps for
the main control panel. Please replace the main control panel if any non-connection problems occur.
The instructions above only apply to designated maintenance professionals.
Locating Main Control Panel Problems

Successful troubleshooting is a precondition of maintenance. Main control panel problems mostly
involve the power supply. As for functional problems, there is no real value in maintenance and the
main control panel should therefore be replaced at your earliest convenience.
There are four steps in locating power-related problems:
1. Look. Power on the panel and pay attention to the power indicator lights. If any abnormality is
spotted, then there is something wrong with the loading of the indicator light’s corresponding
power source. Light abnormality here refers to situations in which a light is off or dimmer than
normal.
2. Smell. Power problems may burn out some components; an acrid smell can suggest a burnout.
Do not power on in this case.
3. Touch. Power on and touch the corresponding component on the main control panel by hand. If
the temperature feels abnormal, the component could be broken.
4. Test. The first three steps can be used to identify power overload problems on the main control
panel. This step is designed for determining what has malfunctioned. Testing can also identify the
problem of circuit breakage. This step is also part of maintenance. A multimeter is used to test for
power problems. The following section lists common problems found in panel maintenance and
their relevant indicators.
Common Problems Found in Main Control Panel Maintenance and their Indicators
See the following table for details:
Problem Description Problem Indicators Solution
Analogy power input of The power indicator light D19 is not This issue is mostly caused by the
-12V is not loaded, and activated. fact that the analog power input of
the device power -12V has shorted out. Another
supply is switched into possible reason is the breakdown
auto protection mode of the polar capacitors (mostly
when powered on. tantalum capacitors here). Due to
the abundance of -12V networks,
users are recommended to replace
the motherboard. The damaged
components can be located by
PCB for replacement by qualified
maintenance personnel.
47
+12V is not loaded, activated. fact that the analog power input of
and the device power +12V has shorted out. Another
supply is switched into possible reason is the breakdown
auto protection mode of the polar capacitors (mostly
when powered on. tantalum capacitors here). Due to
the abundance of +12V networks,
-5V is not loaded lit up or looks dim, and the power fact that the analog power input of
chip U3 feels very hot. -5V has shorted out. Another
possible reason is the breakdown
of the polar capacitors (mostly
tantalum capacitors here). Due to
the abundance of -5V networks,
+5V is not loaded lit up or looks dim, and the power fact that the analog power input of
chip U2 feels very hot. +5V has shorted out. Another
possible reason is the breakdown
of the polar capacitors (mostly
tantalum capacitors here). Due to
the abundance of +5V networks,
The ARM system on The powered-on device fails to do The power management chip U11
the main control panel self-test; the ARM status indicator may be damaged; it is
is not running light D79 is not flashing and the recommended that a qualified
indicator light for ARM power maintenance person replace U11.
management chip D14 is lit up.
The ARM system on The powered-on device fails to do Some circuit in the power
the main control panel self-test; the ARM status indicator management chip U11 may have
is not running light D79 is not flashing and the shorted out. Qualified maintenance
indicator light for ARM power personnel can use a multimeter to
management chip D14 is on. test the power output testing points
throughout U11 to locate the
problematic circuit. Start from the
capacitor and move along until
locating the short circuit.
48
The ARM system on The powered-on device fails to do The boot media for the main
the main control panel self-test; the ARM status indicator control panel, NAND Flash U107,
is not running light D79 is not flashing and the may be damaged; replace the main
indicator light for ARM power control panel as soon as possible.
management chip D14 is on. A
multimeter is used to test the power
management chip only to find that
the power output is normal
throughout the chip and the device
lifetime can last for more than three
years.
The ARM system on The powered-on device fails to do The ARM of the main control panel
the main control panel self-test; the ARM status indicator is damaged; replace the main
is not running light D79 is not flashing and the control panel immediately.
indicator light for ARM power
management chip D14 is on. A
multimeter is used to test the power
management chip only to find that
the power output is normal
throughout the chip and the ARM
chip U50 feels hot.
The FPGA program The FPGA running status indicator The FPGA on the main control
fails to load light, D90, is not flashing, the FPGA panel is damaged; replace the
3.3V power indicator light D17 is in main control panel immediately.
normal ON status, and so is the
FPGA 2.5V power indicator light
D16. FPGA 1.8V power indicator
light D15 is normally lit up; test the
FPGA1.2V with a multimeter and
the voltage tested at TP17 is
normal.
The FPGA program The FPGA running status indicator The FGPA 3.3V power circuit is
fails to load light, D90, is not flashing, and the problematic and the issue can be
FPGA 3.3V power indicator light located by multimeter testing.
D17 is not lit up.
D16 is not lit up.
D15 is not lit up.
The FPGA program FPGA running status indicator light, The FGPA 1.2V power circuit is
fails to load D90, is not flashing; test the FPGA problematic and the issue can be
1.2V power with a multimeter, and located by multimeter testing.
the voltage tested at testing point
TP17 is not 1.2V.
49
Liquid has been spilled The main control panel is eroded. Replace the main control panel
onto the main control
panel.
The driver unit is not The hydraulic tubes have collapsed. Some valves' drivers may have
working properly. malfunctioned; it is suggested that
the panel be replaced as soon as
possible. If the problem persists,
check the driver path for valves,
since this problem is usually
caused by wiring issues or a
broken valve.
The driver unit is not The pump is not working. First confirm the reliability of the
working properly. connection between the driver
panel and the rear panel. If the
connection is reliable, then the
pump may have a problematic
driver; it is suggested that the panel
be replaced right away. If the
problem persists, check the driver
path for pumps, since this problem
is mostly caused by wiring issues
or a broken pump.
The driver unit is not The motor is not working. First confirm the reliability of the
working properly. connection between the driver
panel and the rear panel. If the
connection is reliable, then the
motor may have a problematic
driver; it is suggested that the panel
be replaced right away. If the
problem persists, check the driver
path for motors, since this problem
is mostly caused by wiring issues.
The driver unit is not The driver panel has no heating. Some heating systems may have a
working properly. problematic driver; it is suggested
that the panel be replaced right
away. If the problem persists,
check the driver path for heating,
since this problem is mostly caused
by wiring issues.
Panel maintenance is time-consuming. In principle, panel maintenance is not performed on-site. If a

panel problem is confirmed on-site, please replace the panel.
50
2.3.2 Other Panels

Except for the above-mentioned main control panel, other panels (including reagent testing panel,
reagent sealing panel, front display panel, LCD screen driver panel) will not be repaired in principle. If
problems with any small panel are found on site, please replace it directly.
Functions of interfaces on other panels are briefly introduced below.
2.3.2.1 Reagent Testing Panel

Reagent testing panel is mainly responsible for testing whether there is or no reagent. Functions of
interfaces on the reagent testing panel are shown in Figure 2-41.
Figure 2-41 Reagent testing panel
1 - Sheath flow pressure signal output 2 - Positive and negative pressure

interface signal output interface
3 - Reagent presence detection interface 4 - Installation position for optical

coupling card port
51
2.3.2.2 Front Panel

The front panel is mainly responsible for indicating the running status of the device, alarm notification
and linking with the manual control of the sampling touch signal switch, as shown in Figure 2-42.
Figure 2-42 Picture of the front panel
1 - Control signal communication interface 2 - Micro-switch interface
2.3.2.3 Display Screen Driver Panel

Display screen driver panel is the driver panel for touch screen, mainly responsible for the data
communication between the user interface and the main control panel. Its interface definition is shown
in Figure 2-43.
Figure 2-43 Picture of display screen driver panel
1 - LCD screen ribbon cable 2 - Signal connection interface for the

interface LCD screen and the main control panel
3 - LCD screen back-light cable 4 - LCD screen back-light output

access interface interface
52
3 Repairs
3 Repairs
3.1 Overview
The repair engineer can repair the analyzer using standard tools. See the following section for
repairing procedures (including the tools needed). If any repairing step requires a validating step, the
repair engineer should strictly follow the procedure and take the validating step.
When following the repairing procedure as specified in this section, the operator should put on rubber
gloves and clean both hands with disinfectant after performing any repair work.
3.2 Preparatory Work before Repairs
3.2.1 Open the Left Side Panel
Purpose
When carrying out common device maintenance and troubleshooting, such as removing the sample
syringe assembly on the left side of the device, reagent testing panel, solenoid valve assembly, etc., it
is necessary to remove the left side panel.
Tools/Spare Parts
#2 (Ph2) Phillips screwdriver
Opening
As shown in Figure 3-1, use #2 (Ph2) cross screw driver to take out two M4×8 cross recessed
pan-head combination screws and four M3×8 cross recessed pan-head combination screws on the
rear panel of device housing, and take off the top panel and back panel.
53
3 Repairs
Figure 3-1 Open the left side panel (1)
1 - Top panel 2 - M4×8 cross recessed pan-head screw

(×2)
3 - M3×8 cross recessed pan-head screw 4 – Back panel

(×4)
As shown in Figure 3-2, use #2 (Ph2) cross screw driver to remove three M3×6 cross recessed
countersunk head screws installed on the back for the left door and take off the left side panel.
Figure 3-2 Open the left side panel (2)
1 - Left side panel 2 - M3×8 cross recessed countersunk head screw (×3)
54
3 Repairs
3.2.2 Open the Right Side Door
Purpose
When carrying out common device maintenance and troubleshooting, such as removing the
preheating bath assembly on the right side of the device, pinch valve, sampling assembly, liquid pump
assembly, impedance counting reaction bath assembly (RBC, WBC), negative pressure chamber,
solenoid valve pump assembly, etc., it is necessary to open the right side door.
Tools/Spare Parts
Slot-type screwdriver
Opening
Insert the slot-type screwdriver into the slot in the right side door lock and rotate 90° counterclockwise,
then manually open the right side door.
3.2.3 Disassembling the Front Panel Cover
Purpose
While performing regular machine maintenance and inspection, the front panel cover needs to be
taken down for disassembling the sample probe, cleaning swab, sampling assembly, or indicator light
panel.
Tools/Spare Parts
#2 (Ph2) Phillips screwdriver
Preliminary Steps
Turn off the power switch on the left side of the device and pull out the power cord plug from the back
panel of the device.
Disassembly
1. As shown in Figure 3-3 and Figure 3-4, loosen the pan-head combination screws and countersunk
head screws on the back panel, left and right side doors and top panel of the device one after
another, then, remove the back panel, left and right side doors and the top panel.
55
3 Repairs
Figure 3-3 Before the top panel, back panel, left and right side panels are removed
Figure 3-4 After the top panel, back panel, left and right side panels are removed
2. As shown in Figure 3-5, take out the six affixing M3×8 cross-recessed countersunk head screws in
order to take off the front panel cover. When removing the front panel cover, make sure to pull out
the wires connected to the display circuit panel on the front panel.
56
3 Repairs
Figure 3-5 Disassembling the front panel cover
1 - Front panel cover 2 - M3×8 cross recessed countersunk head screw (×6)
3.3 Display Screen Assembly Replacement

Purpose
The display screen can be disassembled and replaced by following the procedures specified in this
section; however, in each case, 3.2 Preparatory Work before Repairs need to be taken before you
begin.
Tools/Spare Parts
 #2 (Ph2) Phillips screwdriver
 Display screen
Disassembly
1. Pull out both interfaces at the shield panel of the LCD screen interface panel, use #2 (Ph2) cross
screw driver to remove four M3×8 cross recessed pan-head combination screws on the shield
panel, take off the shield panel, then detach the black buckles on both ends, gently pull out the
golden ribbon cable, as shown in Figure 3-6.
57
3 Repairs
Figure 3-6 Replacement of display screen (1)
1 - Bunch interface 2 - LCD screen interface panel
3 - M3×8 cross recessed pan-head 4 - Ribbon cable interface

combination screw (×4)
2. Use #2 (Ph2) cross screw driver to remove eight M3×8 cross recessed pan-head combination
screws on the screen support, take off the screen support, display screen and touch screen
respectively, as shown in Figure 3-7.
Figure 3-7 Replacement of display screen (2)
1 - Touch screen 2 - Display screen
3 - Screen support 4 - M3×8 cross recessed pan-head combination

screw (×8)
58
3 Repairs
3.4 Sampling Assembly Replacement
3.4.1 Replacing the Sample probe
Purpose
The sample probe can be disassembled and replaced by following the procedures specified in this
begin.
Tools/Spare Parts
 Sample probe
Disassembly
1. Pull out the swab's snap ring to detach it from the lower bracket panel. See Figure 3-8 and Figure
3-9.
Figure 3-8 Replacement of sample probe (1)
1 - Cleaning swab 2 - Swab circlip

2. Use a #2 (Ph2) Phillips screwdriver to take out the two M3×6 small cross recessed pan head
combination screws on the fixed pressure panel of the sample probe, and then take off the fixed
pressure panel. See Figure 3-9.
59
3 Repairs
Figure 3-9 Replacement of sample probe (2)
2 - Pressure panel affixing the sample

1 - Sample probe
probe
3 - M3×8 cross recessed small

pan-head combination screw (×2)
3.4.2 Replacing the Optocoupler
Purpose
The optocoupler can be disassembled and replaced by following the procedures specified in this
begin.
Tools/Spare Parts
 2.5mm hex-socket screwdriver
 Optocoupler
Disassembly
Use the 2.5mm hex wrench to remove the M3 hex screw affixing the optocoupler, then pull out the
plug attached to the optocoupler wire to disassemble the optocoupler to be replaced. See Figure 3-10.
60
3 Repairs
Figure 3-10 Replacement of optocoupler
1 - Optocoupler 2 - Sampling assembly
3.4.3 Replacing the Sampling Assembly in X- or Y-direction
Purpose
The sampling assembly can be disassembled and replaced by following the procedures specified in
this section; however, in each case, 3.2 Preparatory Work before Repairs need to be taken before
you begin.
Tools/Spare Parts
 diagonal cutting pliers
Disassembly
1. Use the diagonal cutting pliers to cut off all the nylon binding tapes affixing the fluid tubes
(aspiration tubes of the sample probe and swab tubes), then pull out the fluid tubes of the sample
probe and the swab to detach them from the sampling assembly.
2. Pull out all the motor wires and optocoupler plug from the horizontal moving module of the sample
assembly, and use a #2 (Ph2) Phillips screwdriver to take out the three M3×6 cross recessed
countersunk head screws affixing the bracket for drag chains (see Figure 3-11) to detach the
sampling-specific drag chain from the sampling assembly.
61
3 Repairs
Figure 3-11 Replacement of the sampling assembly in X- or Y-direction (1)
1- tank drag chain connector 2 - M3×6 cross recessed countersunk head screw (×3)
3. Use a #2 (Ph2) Phillips screwdriver to take out the four M4×10 cross recessed pan head
combination screws affixing the sampling assembly, and then carefully and gently take the
sampling assembly out of the machine; during the process of removal, carefully pull out the wires
and plugs for the horizontal motor and optocoupler of the sampling assembly from the
corresponding apertures in the machine. Then pull out all the plugs to completely remove the
sampling assembly. See Figure 3-12.
Figure 3-12 Replacement of the sampling assembly in X- or Y-direction (2)
1 - Sampling assembly 2 - M4×10 cross-recessed pan-head combination screw (x4)
62
3 Repairs
3.5 Power Assembly Replacement

Purpose
The power assembly can be disassembled and replaced by following the procedures specified in this
begin.
Tools/Spare Parts
 Power assembly with the same specifications
Disassembly
1. Use #2 (Ph2) cross screw driver to take out four M3×8 cross recessed pan-head combination
screws on the back of the device power supply assembly, then remove one M3×8 cross recessed
pan-head combination screw on the front, as shown in Figure 3-13.
Figure 3-13 Replacement of power assembly
1 - Power assembly 2 - M3×8 cross-recessed pan-head

2. Pull out all lead plugs connected to the power supply.
3. Take off the power supply required to be replaced from the power supply assembly, and replace
with a new power supply, fix it with screws.
63
3 Repairs
3.6 Hydraulics Components incl. Valves, Pumps, and

Pressure Chambers Replacement
3.6.1 Replacing the valve assembly
Purpose
The valve assembly can be disassembled and replaced by following the procedures specified in this
begin.
Tools/Spare Parts
 The replacement valve with the same specifications
Preliminary Steps
1. Turn off the power switch on the left side of the device.
2. Pull out the power cord plug from the rear panel of the device.
3. If it is only the electromagnetic valve on the left or right clapboard that needs to be replaced, open
the corresponding left- or right-side door; if the one on the front panel needs to be replaced,
remove the front panel cover by following the instructions in 3.2.3 Disassembling the Front
Panel Cover.
Disassembly
1. Disassemble the peripheral fluid tubes connected to the valve assembly.
2. Use a #2 (Ph2) Phillips screwdriver to disassemble the two M3×8 cross-recessed pan-head
combination screws which affix the valve assembly, and carefully take out the valve assembly
while making sure to unplug the attached wires. See Figure 3-14.
Figure 3-14 Replacement of valve assembly
1 - the corresponding replacement valve 2 - Installation panel affixing the valve
3 - M3×8 cross-recessed pan-head

64
3 Repairs
Installation
 Make sure to the use correct model of valve and create a reliable connection
 All the wirings need to be set up according to the original positions of the machine components to
prevent them from being crushed or otherwise damaged.
 This machine involves a great number of valves. Only one valve is discussed as an example here,
and all the other valves follow the same maintenance procedures.
Follow the corresponding disassembly steps in reverse order.
3.6.2 Replacing the Liquid Pump Assembly
Purpose
The liquid pump assembly can be disassembled and replaced by following the procedures specified in
you begin.
Tools/Spare Parts
 The replacement liquid pump with the same specifications
Preliminary Steps
1. Turn off the power switch on the left side of the device.
2. Pull out the power cord plug from the rear panel of the device.
3. Open the right side door.
Disassembly
1. Pull out the peripheral fluid tubes and wire connectors attached to the liquid pump.
2. Use a #2 (Ph2) Phillips screwdriver to loosen the two M4×10 cross-recessed pan-head
combination screws which affix the liquid pump, and move the liquid pump assembly to the left till
the big hole on the affixing panel of the liquid pump is aligned to the affixing screw, then carefully
hold up the liquid pump assembly to take it out. See Figure 3-15.
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3 Repairs
Figure 3-15 Replacement of liquid pump assembly (1)
1 - Liquid pump 2 - M4×10 cross-recessed pan-head

assembly combination screw (×2)
3. The further dismantling of the liquid pump assembly is shown in Figure 3-16.
Figure 3-16 Replacement of liquid pump assembly (2)
1 - Liquid pump 2- M3×8 stainless-steel inner hex screw (×8)
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3 Repairs
Installation
 All the wirings need to be set up according to their original positions to prevent them from being
crushed or otherwise damaged, and to prevent the working liquid pump from being shaken, which
affects the fluid tubes.
 Pay special attention to the tubing joints and ensure that the connections are sound.
Resetting the Machine

1. Close the right side door.
2. Plug the power cord into the back panel of the device and turn on the power switch on the left
3.6.3 Replacing the Negative-pressure Chamber Assembly
Purpose
The negative-pressure chamber assembly can be disassembled and replaced by following the
procedures specified in this section; however, in each case, 3.2 Preparatory Work before Repairs
need to be taken before you begin.
Tools/Spare Parts
 The replacement parts or assembly of the negative-pressure chamber with the corresponding
specifications
Preliminary Steps
Open the right side door.
Disassembly
1. Disassemble the peripheral fluid tubes connected to the negative-pressure chamber.
2. Use a #2 (Ph2) Phillips screwdriver to disassemble the two M4×10 cross-recessed pan-head
combination screws which affix the negative-pressure chamber, and carefully take out the
negative-pressure chamber assembly. See the picture below.
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3 Repairs
Figure 3-17 Replacement of negative-pressure chamber assembly
1 - Negative-pressure 2 - M4×10 cross-recessed pan-head

chamber assembly combination screw (×2)
3. The further dismantling of the negative-pressure chamber assembly is the same as that of the
positive-pressure chamber assembly.
Installation
 Make sure to place the seal ring into the seal tank to keep the pressure chamber airtight.
 All the tubing needs to be set up according to the relevant standards or the original positions of
the machine components to prevent them from being crushed or otherwise damaged.

Close the right side door.
3.7 Sheath Flow Syringe Assembly Replacement
3.7.1 Replacing the syringe
Purpose
The syringe can be disassembled and replaced by following the procedures specified in this section;
however, in each case, 3.2 Preparatory Work before Repairs need to be taken before you begin.
Tools/Spare Parts
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3 Repairs
 The replacement syringe assembly with the same specifications
Preliminary Steps
Open the left side door.
Disassembly
1. Pull out the peripheral fluid tubes connected to the syringe assembly to be replaced.
2. Use the 2.5mm hex-socket screwdriver to disassemble the two M3×8 stainless-steel inner hex
screws affixing the syringe assembly. Then take out the syringe assembly. See the picture below.
Figure 3-18 Replacement of syringe
1 - Syringe assembly 2- M3×8 stainless-steel inner hex screw (×2)
Installation
3.7.2 Replacing the Motor
Tools/Spare Parts
 The replacement syringe assembly with the same specifications
Preliminary Steps
Open the left side door.
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3 Repairs
Disassembly
1. Pull out the peripheral fluid tubes connected to the syringe module to be replaced.
2. As shown in Figure 3-19, use #2 (Ph2) cross screw driver to remove four M3×8 cross recessed
pan-head combination screws, take off the syringe assembly.
Figure 3-19 Replacement of motor
3. Use the 2.5mm hex-socket screwdriver to disassemble the two M3×8 stainless-steel inner hex
screws affixing the syringe module. Then take out the syringe.
4. Take out the two M3×8 inner hex screws on the shield cover, then remove the white bolt from the
motor. Next, remove the four M3×8 inner hex screws from the back of the motor for the
replacement.
1 - Motor bolt 2 - Motor
3 - M3×8 stainless-steel inner hex screw (×9)
Installation
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3 Repairs
3.8 WBC and RBC Bath Assemblies Replacement
3.8.1 Dismantling and replacing the WBC bath assembly
Purpose
The RBC counting bath assembly can be disassembled and replaced by following the procedures
specified in this section; however, in each case, 3.2 Preparatory Work before Repairs need to be
taken before you begin.
Tools/Spare Parts
 The replacement WBC counting bath assembly with the same specifications
Preliminary Steps
1. Run the draining sequence of the software to drain any residual liquid inside the machine.
2. Turn off the power switch on the left side of the device and pull out the power cord plug from the
back panel of the device.
Disassembly
1. Use a #2 (Ph2) Phillips screwdriver to disassemble the M3×8 cross-recessed pan-head
combination screw in the lower section of the shield cover to remove the cover; to take out the
screw, move slightly upwards for 3mm or so and apply force in a direction perpendicular to the
right clapboard. See Figure 3-20.
Figure 3-20 Dismantling and replacing the WBC bath assembly (1)
1 - Shield cover of the counting 2 - Cross-recessed pan-head combination

bath screw
2. Pull out the peripheral fluid tubes attached to the WBC counting bath assembly. Pay attention to
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3 Repairs
handling the residual fluid; try to prevent it from flowing onto the other components in the machine,
and wipe clean if necessary.
combination screw for grounding on the middle clapboard of the shield case cover and pull out the
grounding wire. See Figure 3-21.
1 - WBC counting chamber assembly 2 - M3×8 cross-recessed pan-head

combination screw
4. Use 2.5mm hex wrench to remove the two M3×8 stainless-steel inner hex screws affixing the
upper section of the WBC counting bath assembly. See Figure 3-22.
1 - WBC counting chamber assembly 2 - M3×8 stainless-steel inner hex screw (×2)
5. Carefully and slowly take out the WBC counting bath assembly. Pay attention to the wiring in the
process of moving to avoid breaking the wires.
6. Remove the protective ring from the shield case and open the wire clip to take out the inner wire
along the stretching direction of the wire. Then take out the WBC counting bath assembly as a
whole.
7. The further dismantling of the WBC counting chamber assembly is shown in Figure 3-23.
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3 Repairs
1 - LED 2 - Front counting bath
3 - Flat rubber gasket 6*4.5*0.5 4 - WBC aperture
5 - Flat rubber gasket 6*3*0.5 6 - O-shaped ring 5.5*1.0
7 - Electrode of the rear bath 8 - O-shaped ring 6.5*1.0
9 - Rear chamber 10 - Optical receiver
11 - Optical filter of the counting bath assembly 12 - HGB bracket
Installation
 All the wirings need to be set up according to the working procedures or the original positions of
 The aperture, seal gasket, and seal ring need to be correctly positioned and oriented in the right
direction.

1. Reinstall the shield case cover.
3.8.2 Dismantling and replacing the RBC bath assembly
Purpose
The RBC counting bath assembly can be disassembled and replaced by following the procedures
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3 Repairs
specified in this section; however, in each case, 3.2 Preparatory Work before Repairs need to be
taken before you begin.
Tools/Spare Parts
 The replacement WBC counting bath assembly with the same specifications
Preliminary Steps
1. Run the draining sequence of the software to drain any residual liquid inside the machine.
Disassembly
combination screw in the lower section of the shield cover to remove the cover; to take out the
screw, move slightly upwards for 3mm or so and apply force in a direction perpendicular to the
right clapboard.
2. Refer to the corresponding section in 3.8.1 Dismantling and replacing the WBC bath
assembly.
3. Pull out the peripheral fluid tubes attached to the RBC counting bath assembly. Pay attention to
handling the residual fluid; try to prevent it from flowing onto the other components in the machine,
and wipe clean if necessary.
combination screw for grounding on the middle clapboard of the shield case cover and pull out the
grounding wire. See Figure 3-24 .
Figure 3-24 Dismantling and replacing the RBC bath assembly (1)
1 - RBC counting chamber 2 - M3×8 cross-recessed pan-head

assembly combination screw
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3 Repairs
5. Use 2.5mm hex wrench to remove the two M3×8 stainless-steel inner hex screws affixing the
upper section of the RBC counting bath assembly. See Figure 3-25.
1 - RBC counting 2- M3×8 stainless-steel inner hex

chamber assembly screw (×2)
6. Carefully and slowly take out the RBC counting bath assembly. Pay attention to the wiring during
the process of moving to avoid breaking the wires.
7. Remove the protective ring from the shield case and open the wire clip to take out the inner wire
along the stretching direction of the wire. Then take out the RBC counting bath assembly as a
whole.
8. The further dismantling of the RBC counting chamber assembly is shown in Figure 3-26.
1 - Front counting bath 2 - Flat rubber gasket 6*4.5*0.5
3 - RBC aperture 4 - Flat rubber gasket 6*3*0.5
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3 Repairs
5 - O-shaped ring 5.5*1.0 6 - Electrode of the rear bath
7 - O-shaped ring 6.5*1.0 8 - Rear chamber
Installation
 The aperture, seal gasket, and seal ring need to be correctly positioned and oriented in the right
direction.

1. Reinstall the shield case cover.
3.9 Main Control Panel Replacement

Purpose
The main control panel can be disassembled and replaced by following the procedures specified in
you begin.
Tools/Spare Parts
 Phillips screwdriver
 Corresponding specifications needed to replace the main control panel
Preliminary Steps
1. Turn off the power switch of the analyzer, and pull out the power cord plug.
2. Disassemble the screws on the back panel of housing and remove the back panel of the housing.
Disassembly
1. Pull out the peripheral wires and connectors attached to the main control panel.
2. As shown in Figure 3-27, disassemble four M4×8 cross recessed pan-head combination screws
fixing the main control panel, and remove the main control panel.
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3 Repairs
Figure 3-27 Replacement of the main control panel
1
2
1 - Main control panel 2 - M3×8 cross-recessed pan-head combination screw (×4)
Installation

1. Assemble the back panel of housing.
2. Plug the power cord into the back panel of the device and turn on the power switch.
3.10 Reagent Testing Panel Replacement

Purpose
The reagent testing panel can be disassembled and replaced by following the procedures specified in
you begin.
Tools/Spare Parts
 The replacement reagent testing panel with the same specifications
Preliminary Steps
2. Open the left side door.
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3 Repairs
Disassembly
1. Remove the four M3×8 cross recessed pan-head combination screws affixing the protective cover
of the reagent testing panel and remove the protective cover. See Figure 3-28.
Figure 3-28 Replacement of reagent testing panel (1)
1- Protective cover 2 - M3×8 cross-recessed pan-head

2. Pull out all the exposed peripheral wires connected to the reagent testing panel.
3. Remove the four M3×8 cross-recessed pan-head combination screws affixing the reagent testing
panel and carefully take out the reagent testing panel. Make sure that the metal parts will not
scratch the wiring at the rear of the panel. Pull out the rear wiring to remove the reagent testing
panel. See Figure 3-29.
Figure 3-29 Replacement of reagent testing panel (2)
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3 Repairs
1 - Reagent detection 2 - M3×8 cross-recessed pan-head

panel combination screw (×4)
Installation
 Pay attention to the position of wires when installing the sensor panel so as to prevent the wires
from breaking.

1. Close the corresponding left side door.
3.11 Temperature Sensor Replacement
3.11.1 Disassembling and Replacing the Temperature Sensor
Purpose
The temperature sensor can be disassembled and replaced by following the procedures specified in
you begin.
Tools/Spare Parts
 Hex-socket screwdriver
 The replacement temperature sensor with the same specifications
Preliminary Steps
2. Open the left side door and the front panel cover.
Disassembly
Loosen the screws in the bracket for temperature sensor to take the sensor out. Then replace it with a
new sensor and tighten the screws. See Figure 3-30.
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3 Repairs
Figure 3-30 Disassembling the temperature sensor
1 - Temperature sensor 2 - Temperature sensor bracket
3 - M3×8 fixation screws
Installation

1. Re-install the front panel cover, and close the corresponding left side door.
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4 Software Upgrade
4 Software Upgrade
4.1 Preparation
1. Get officially provided upgrade package, and prepare a USB flash disk for storing the upgrade
package.
 Please adopt USB flash disks manufactured by legitimate manufacturers.

 The USB flash disk capacity shall be larger than 1G, and the format is FAT32.
2. Copy the upgrade package to the root directory of the USB flash disk (Do not paste it into any
newly-created folder).
3. Insert the USB flash disk with upgrade package into the USB interface of the host.
4. Use service authority to log in to the host.
4.2 Upgrading Steps

1. Enter the Service interface, and click the Upgrade button, as shown in the picture below.
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4 Software Upgrade
2. The host will detect the USB flash disk. 2~5 seconds later, if the USB flash disk cannot be
recognized, there will be corresponding prompt in the interface, please insert the USB flash disk
again; if the USB flash disk can be recognized, there will be dialog box in the interface as follows.
3. Select the upgrade file upgrade_1001_vxxx_XXXXXXXXXXXX.tar, and click Yes.

vxxx indicates the version of the upgrade file, and XXXXXXXXXXXX indicates the release date of
the upgrade file.
The system will pop up a similar interface as follows, listing the modules able to be upgraded and
their versions.
Meaning of the name of each module:

 LS: System boot program version
 OS: Operating system version
 HFPGA: FPGA program version
 DMCU: MCU program version
 AS: Application version
4. Click Yes to start the upgrade; click No to cancel the upgrade and restart the analyzer.
There will be prompt with the upgrading progress on the screen. If the upgrade is successful, the
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4 Software Upgrade
progress bar will display 100%. See the picture below.
5. Turn off the power supply of the analyzer, and restart the analyzer.
4.3 Touch Screen Calibration

After replacing with a new touch screen, it is necessary to calibrate the touch screen prior to use,
steps for calibration are as follows:
1. Prepare a USB flash disk with FAT32 format, create a new ts.txt file under its root directory.
2. Insert the USB flash disk into the USB interface of device.
3. Turn on the device power supply, and start the analyzer.
4. When the login interface is displayed on the LCD screen, press and hold the sample Aspirate key
for more than 15s.
5. There will be black calibration interface displayed on the LCD screen, as shown in the picture
below.
6. Use a hard object, such as a toothpick or pencil to click the focal points successively appearing on
the screen (5 points in total).
After clicking 5 focal points, the touch screen calibration is complete.
7. Turn off the power supply of the analyzer, and then restart the analyzer.
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5 Comprehensive Device Tuning
Since the replacement and maintenance of some components can lead to changes in relevant tuning
parameters, this section introduces the tuning procedures for the parameters that may be affected.
5.1 Position Adjustment
The swab height needs to be reset after the replacement of the sampling assembly and
relevant parts of the sampling swab.
When the sampling assembly or swab is replaced, it is necessary to carry out the tuning as follows:
1. Enter the Service screen and click Position Adjustment.
2. Click Start and check the distance between the bottom of the sample probe and the bottom of the
swab. If the sample probe is lower than the swab, click Up; if higher, click Down. Each click will
move the sample probe slightly. In the end, the bottom of the sample probe will be aligned with the
bottom of the swab. Then click OK.
3. Click Check to run the initialization of the sample probe. Check if the bottom of the sample probe
is still aligned with the bottom of the swab (see Figure 5-1). If it is, click OK to complete the tuning;
84
if not, repeat Steps 1~2.

Figure 5-1 Position Adjustment
5.2 HGB Voltage Gain Setting
The HGB voltage gain setting needs to be performed for the replacement of WBC bath, HGB
wire and/or main control panel.
1. In the Setup > Meterage screen, click Gain Settings.

2. Click the up/down adjustment button after the filling blank for HGB value and HGB background
voltage value will change accordingly within 4.5+/-0.3V.
3. Click OK (see Figure 5-2).
Figure 5-2 HGB voltage gain setting
5.3 Gain Calibration
Gain calibration needs to be performed for the replacement of WBC bath, RBC bath, and/or
main control panel.
85
1. Click Service > Cal > Gain cal. to access the gain calibration screen. See Figure 5-3.
Figure 5-3 Gain calibration
2. Fill in the Target cells corresponding to W-MCV&MCV with W-MCV and MCV reference values for
quality control.
3. Perform the QC test for three times in a row for the first run.
The results for each time will be automatically displayed.
 If the CV falls within reasonable parameters, the screen will show the CV and Gain values for
the first run. Go to step 4.
 If it does not fall within reasonable parameters, you’ll be prompted to redo the calibration.
Please click OK to close the message box. Then click Clear to delete the data, and repeat the
step 3.
4. Perform the QC test for three times in a row for the second run.
The results for each time will be automatically displayed.
 If the CV falls within reasonable parameters, the screen will show the CV and Gain values for
the second run, and show the final results. Please click "OK" to complete gain calibration.
 If it does not fall within reasonable parameters, you’ll be prompted to redo the calibration.
Please click OK to close the message box. Then click Clear to delete the data, and repeat the
step 4.
5.4 Calibration of Calibrators
The calibrators need calibration for the replacement of the WBC bath, the RBC bath, and/or the
main control panel.
5.4.1 Calibration in Whole-blood Mode

1. In standby mode, click Cal to enter the calibration screen.
2. Select Calibrator (see Figure 5-4).
86
Figure 5-4 Auto Calibration Using Calibrators
3. Input the calibrator lot No. JZQX-01 in the text box for Lot No., and click the Exp. Date control to
set the expiry date of the calibrator.
4. Input the target value of the current calibrator parameter.
The calibration mode is Whole Blood by default, so there is no need to set the mode.
5. Set the well-shaken calibrator under the sample probe, and then click the aspiration key on the
analyzer to start the calibration counting.
6. Repeat step 5 for a total of 12 times to get 12 results of calibration counting.
 After the counting is complete, a dialog box will pop up indicating that the test is complete.
Click the Save button to save the calibration result.
 If significant differences are found across results, a dialog box for data abnormality will pop up.
Please redo the calibration.
5.4.2 Calibration in Predilute Mode

1. In standby mode, click Cal to enter the calibration screen.
2. Select Predilute on the right side of the screen.
3. Input the calibrator lot No. JZYXS-01 in the text box for Lot No., and click the Exp. Date control to
set the expiry date of the calibrator.
4. For calibration using the prediluted calibrators, please refer to steps 4~6 in whole-blood mode.
5.5 LIS Connection

If the analyzer needs to be connected to laboratory information system (hereinafter referred to as LIS),
you can complete the connection by following the steps in this section.
5.5.1 Installing LIS Workstation

1. Install LIS workstation and set instrument type and model.
87
2. Enter LIS workstation network setup interface after installation and set monitoring IP address and
port number.
Refer to Description of LIS Communication Protocol for Dymind Hematology Analyzers to

complete the support of the LIS workstation to the LIS communication Protocol.
5.5.2 Host Communication Settings

1. Use a network cabel to connect the analyzer to LIS local area network.
2. Log on the auto hematology analyzer software; if done, skip this step.
The whole process lasts for 4 to 12 minutes. Please be patient.
3. In the Setup interface, click Host Communication in the Communication selection to access
the Laboratory Information System (LIS) communication setting interface.
See Figure 5-5.
Figure 5-5 Host Communication Settings
4. Set the IP address and other network information of the analyzer according to the actual situation.
 If the network is accessed through a router on the site, please select Obtain an IP address
automatically and Obtain DNS server address automatically.
88
 If the network is accessed through a network switch, or the analyzer is directly connected to
the LIS on the site, please select Use the following address, so as to manually set the IP
address and subnet mask of the analyzer. The IP addresses of the analyzer and LIS must be
in the same network segment. Furthermore, their subnet masks shall be the same, while other
parameters can maintain null.
5. Click OK to save the settings and close the dialog box.
5.5.3 Connecting Analyzer with LIS

1. Log on the auto hematology analyzer software; if done, skip this step.
The whole process lasts for 4 to 12 minutes. Please be patient.
2. In the Setup interface, click LIS Communication in the Communication selection to access the
Laboratory Information System (LIS) communication setting interface.
See Figure 5-6.
Figure 5-6 LIS Communication Settings
3. Input the IP address and port of LIS workstation in Network Settings area.
Find the IP address and port of LIS in the network setup interface in the LIS workstation; if IP
address can’t be found, try the method below:
a. Enter the operating system of LIS workstation.
b. Press combination key [Windows+R] to open the Run window.
c. Input cmd, and then click OK.
d. Input the ipconfig command into the cmd.exe window popped out.
The interface shows similar content as follows:
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The IPv4 address in the red box is the IP address of LIS workstation.
The IP address 192.168.8.44 of the LIS workstation shown as above is used as an example, real IP
should be in the same network segment with LIS server.
4. Click OK to save the settings.

5. Check if the connection is successful.
The LIS icon in the upper right side on the analyzer screen turns from gray to black ,
which indicates auto hematology analyzer software is connected to LIS successfully.
If the icon stays gray, the connection fails. Please check if the IP address and port of LIS is correct
and reconnect as the steps above; if the problem still exists, please contact the hospital network
administrator to handle it.
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6 Alarms and Solutions

This section introduces error messages that can appear in the analyzer, possible causes, and
troubleshooting steps to be taken by the operator. If the problem persists after troubleshooting, please
take hardware issues into account and consider replacing the relevant parts or panels.
For the following issues, please click the error message box at the bottom right corner of the software
interface, then click "Remove Error" in the popup dialog box. Usually the problem will be automatically
resolved; if it persists, refer to the "Solution" column for further maintenance.
Problem Solution
No. Problem Description
Name
1. This syringe problem can occur while it is

The syringe fails to leave its being moved. Please refer to the following
initial position. solution:
2. Follow the instructions in 3.7 Sheath Flow
Sample syringe fails to return Syringe Assembly Replacement to
to its initial position. disassemble the syringe, then remove the dust
cover and optocoupler. Plug the optocoupler
into the connector of the syringe optocoupler.
The sample syringe takes too
The user enters the Service > Status >
many steps to return to its
Syringe Sensor screen and covers the center of the
1 initial position.
problem optocoupler with a piece of paper. Check if the
optocoupler status shown in the screen is
blocked; if yes, then the optocoupler is working
The sample syringe is busy.
properly.
3. Follow the instructions in 3.7.2 Replacing the
Motor to disassemble the syringe motor and
replace it with a new motor. Then go to syringe
Sample syringe timeout self-test under Service > Self-test > Syringe
Self-test; if the syringe is working, then the
maintenance has been successful.
The horizontal motor fails to 1. The user enters the Service > Status >
leave its initial position. Sensor screen and covers the center of the
optocoupler with a piece of paper. Check if the
The horizontal motor fails to optocoupler status shown in the screen is
return to its initial position. blocked. If it is, then the optocoupler is working
Horizontal properly; if not, then refer to 3.4.2 Replacing
The motor fails to move to the
2 motor WBC position. the Optocoupler on how to replace the
problem optocoupler.
The motor fails to move to the 2. Refer to 3.4.3 Replacing the Sampling
open sampling position. Assembly in X- or Y-direction to disassemble
the sampling assembly and make sure the
The horizontal motor is busy.
motor wiring is secure. If the wiring is OK, then
Horizontal motor timeout disassemble the motor and replace it with a
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Problem Solution
Name
new one. After the installation, make proper
adjustments to the belt's tension, then go to
The optocoupler of the the sampling assembly self-test under
horizontal motor is not Service > Self-test > Syringe Self-test. If the
working properly. sampling assembly is working, then the
maintenance process is OK.
The vertical motor fails to 1. The user enters Service > Status > Sensor
leave its initial position. screen and covers the center of the vertical
optocoupler with a piece of paper. Check if the
The vertical motor fails to corresponding optocoupler status shown in the
return to its initial position. screen is blocked. If it is, then the optocoupler
The motor fails to move into is working properly; if not, then refer to 3.4.2
position to isolate the air Replacing the Optocoupler on how to
bubbles. replace the optocoupler.
Vertical 2. Refer to 3.4.3 Replacing the Sampling
3 motor The motor fails to move to the Assembly in X- or Y-direction to disassemble
problem counting bath position. the motor from the sampling assembly and
make sure motor wiring is secure. If the wiring
The motor fails to move to the
is OK, then disassemble the motor and replace
open sampling position.
it with a new one. After the installation, make
The vertical motor is busy. the proper adjustments to the belt tension,
then go to the sampling assembly self-test
under Service > Self-test > Syringe Self-test.
Vertical motor timeout If the assembly is working, then it has been
successfully fixed.
The CC Refer to 3.9 Main Control Panel Replacement

source The CC source voltage is to replace the main control panel.
4
voltage is abnormal.
abnormal
The 12V +12V power is not working Refer to 3.9 Main Control Panel Replacement
power is properly. to replace the main control panel.
5
not working -12V power is not working
properly properly.
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Problem Solution
Name
1. Take another measurement after performing

the soaking with probe cleanser to see if the
problem has been resolved. If not, enter into
the system for multiple times to check HGB
background voltage. If the voltage is steady
and exceeds the standard ratings, please
perform the following procedures.
Abnormal HGB background
2. Go to Setup > Meterage > Gain Settings
voltage.
screen, adjust the current HGB gain and set
(The rated range of HGB background voltage within 4.5+/-0.1V.
Abnormal background voltage is
6 background 3. If the problem persists, please try to clean the
4.2~4.8V; a system message
voltage transmitting end and the receiving end of the
abnormal background
HGB bracket. These two areas should not be
voltage will be shown to
cleaned with alcohol or organic solvents.
remind the user to adjust
Instead, use a rubber pipette bulb for purging.
HGB gain.)
If the counting bath is contaminated with
overflown liquid, wipe it clean with tissue paper
that will not flake.
4. If the problem persists after the above steps
are taken, please consider replacing any
relevant components such as the HGB bracket
or analog panel.
Check if the ambient temperature is within the

Abnormal specified range of 15~30°C; if yes, refer to 3.11.1
The ambient temperature
7 temperatur Disassembling and Replacing the
exceeds the working range.
e Temperature Sensor to replace the temperature
sensor.
The positive-pressure Refer to 2.2.11.9 Problems with Creating

chamber fails to create Positive Pressure for troubleshooting.
pressure
Abnormal pressure of
positive-pressure chamber
(lower than normal)
positive-pressure chamber
(higher than normal)
Pressure
8 chamber The negative-pressure Refer to 2.2.11.10 Problems in Creating the
problem chamber fails to create Negative Pressure for troubleshooting.
pressure
negative-pressure chamber
(lower than normal)
negative-pressure chamber
(higher than normal)
DIFF probe clogging.
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Problem Solution
Name
1. Check if the reagent has expired or is

contaminated.
2. Go to the Service > Maintenance > Maintain
screen and click cleanser soaking to clean the
hydraulics. Then return to the Sample
Analysis screen and conduct background
measurements to see if the problem has been
Abnormal background.
resolved.
Abnormal (One or multiple results of
9 3. If the problem persists, please check for any
background background measurement
peripheral interference from the grounding wire
exceed the background
or shield wire, or if any electric-brush devices
range.)
such as drills are being used on and off in the
area. This can influence the counting results.
4. If there is no such interference, please check
the airtightness of each syringe and the rear
chamber of the counting bath. If the
airtightness is unsatisfactory, then replace
accordingly.

screen and click Unclog to start unclogging.
2. Go to the Service > Self-test > Valve/Pump
Abnormal Self-test screen, click Valve 7 and check if it is
10 WBC WBC clogging. working. If it is OK, please refer to 2.2.11.4
counting Aperture Clogging of the WBC Channel for
operation.
screen and click Cleanser Soak.

screen and click Unclog to start unclogging.
2. Go to the Service > Self-test > Valve/Pump
Abnormal Self-test screen, click Valve 7 and check if it is
11 RBC RBC clogging. working. If it is OK, please refer to 2.2.11.5
counting Aperture Clogging of the RBC Channel for
operation.
screen and click Cleanser Soak.
Check the connection of the float sensor at the

Waste rear section of the machine; if the connection is
12 Waste container is full.
overflow OK, replace the float sensor for waste overflow
detection.
DIL-E expiration. Expired reagent or insufficient residual amount.

This means that the reagent has gone out of date
Insufficient DIL-E. or the amount remaining is insufficient to support
Abnormal hydraulics operations such as counting. Please
13 DIL-E is not replaced.
reagent following the troubleshooting procedures below:
LYE-1 expiration. Go to the Reagent Management > Setup
screen, scan the barcode of the new reagent as
Insufficient LYE-1.
94
Problem Solution
Name
shown in the alarm message, and then load the
LYE-1 is not replaced. reagent to resolve the problem.
DIL-E running out or air Check for the availability of the relevant reagent.
bubbles in inlet tubing. If the reagent is available, then check if the
optocoupler of the reagent testing panel is on. If
not, adjust the screw of the potentiometer and
rotate counterclockwise to see if the optocoupler
can be activated.
 If it works, drain the liquid from the tube
and use a multimeter to press the spot as
shown in the picture. Adjust the
potentiometer and set the voltage at 1.5V
or so (the testing points are all located in
the left part of the picture, among which
Potentiometer A corresponds to Testing
Point A, and so on and so forth).
14 No reagent
LYE-1 running out or air
bubbles in inlet tubing.
 If it is not working, refer to 3.10 Reagent

Testing Panel to replace the reagent
testing panel.
Check if the right-side door is open. The door

Door or
should not press against the microswitch. If the
15 cover open The right-side door is open.
door is properly closed, please replace the
error
microswitch on the side door.
95
7 Maintenance Inventory
No. Material No. Material Name
1 20.01.0071A Power assembly
2 20.01.0143A WBC counting bath assembly
3 20.01.0144A RBC counting bath assembly
4 20.01.0022A Reagent presence testing PCBA
5 20.01.0070A 1001 syringe assembly
6 20.01.0068A Sampling assembly
7 20.01.0055A Negative-pressure chamber assembly
8 22.01.0023A PCBA of the main control panel (1001)
9 22.01.0020A LCD screen interface panel PCBA
10 23.99.0001A Wire for the 2-way electromagnetic valve (L)
11 23.99.0002A Wire for the 3-way electromagnetic valve (L)
12 23.99.0003A Wire for the 2-way electromagnetic valve (S)
13 23.99.0004A Wire for the 3-way electromagnetic valve (S)
14 23.99.0007A Wire for the location optocoupler
15 23.99.0008A Wire for the liquid pump
16 23.99.0015A Wire for sample aspiration micro-switch
17 23.99.0016A Wire for the temperature sensor
18 23.99.0017A Wire for the self-control motor
19 23.99.0018A Wire for the side door microswitch
20 23.99.0020A Belt pulley
21 24.02.0005A Stepping motor
22 24.05.0004A Synchronous belt (TBN192MXL025)
23 24.05.0002A Synchronous belt (TBN140MXL025)
24 37.01.0010A Control wire for AD input
25 37.01.0021A Grounding wire for the rear panel
26 37.02.0040A Liquid pump control wire

27 37.02.0038A Control wire for the float sensor
96
No. Material No. Material Name

28 37.02.0034A Control wire for the sampling syringe
29 37.02.0035A Control wire for the horizontal/vertical stepping motor
30 37.02.0037A Control wire for the syringe optocoupler
31 37.02.0036A Control wire for the sampling optocoupler
32 37.02.0041A Control wire for the temperature sensor
33 37.02.0031A Valve control wire 1
34 37.02.0032A Valve control wire 2
35 37.02.0055A Control wire for the front panel cover
36 37.02.0049A Reagent control wires
37 53.09.0003A Guide shaft
38 53.20.0001A Towing-chain connectors
39 53.99.0001A Towing chain
40 56.01.0063A Cleansing swabs
41 56.01.0163A Open sample probe (Needle open)
42 60.01.0012A Isolation chamber
43 60.01.0027A Aspiration touch panel (black)
44 60.10.0025A DH3 series screen-printing housing
45 63.01.0023A Double-layered tube (1000mm) for maintenance
46 63.01.0024A EVA tube (1100mm) for maintenance
47 63.01.0025A Slim No. 50 tube (1000mm) for maintenance
48 63.01.0026A Wide No. 50 tube (850mm) for maintenance
49 63.01.0027A No. 3603 tube (1500mm) for maintenance
50 63.01.0028A MPF tube (1000mm) for maintenance
51 63.01.0029A 1.5mm Teflon tube (1100mm) for maintenance
52 63.01.0030A 1.0mm Teflon tube (1000mm) for maintenance
53 63.01.0031A 2.4mm TPU rubber tube (300mm) for maintenance
54 63.01.0032A 0.78mm silicone tube (50mm) for maintenance
55 63.01.0033A Pharmed tube (50mm) for maintenance
56 63.01.0034A 1.6mm thin silicone tube (40mm) for maintenance
57 63.01.0035A 1.6mm thick silicone tube (80mm) for maintenance
58 63.01.0036A 3.2mm silicone tube (200mm) for maintenance
97

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3.4.3 Replacing the Sampling Assembly in X- or Y-direction .................................................... 61

1.1 Who Should Read This Manual

1.2 Symbols and Legends

The operator should follow the instruction below each symbol to

The operator should follow the instructions below each symbol to

The operator should follow the instructions below each symbol to

When you see It means

Exercise caution to prevent puncture

Instruction for Moving

Alternating current (AC)

In Vitro diagnostic medical device

European CE declaration of conformity

When you see It means

Authorized Representative in the European Community

Atmospheric pressure limitation

Consult the instructions for use

Keep away from sunlight

Let this side face upward.

Fragile, handle with care

The analyzer, after being scrapped, should not be disposed

1.3 Safety Instructions

2.1 Mechanical Components

1 - Sample aspiration touch panel 2 - Sample probe

3 - Status indicator light 4 - Thermal printer

Figure 2-2 Analyzer Profile

1 - LAN network interface 2 - USB interface

Figure 2-3 Back view of the analyzer

1 - Power switch 2 - AC input

3 - Cooling fan 4 - Diluent inlet connector

5 - Waste outlet 6 - Waste level detection

7 - Diluent presence detection connector 8 - Ground studs

1 - Sampling assembly 2 - Main control panel

Figure 2-5 Right-side view of the analyzer (right-side door removed)

1 - Power assembly 2 - Sampling assembly

3 - Counting bath assembly 4 - Electromagnetic valve assembly

5 - Negative-pressure chamber 6 - Liquid pump assembly

Figure 2-6 Left-side view of the analyzer (Left-side door removed)

1 - Power assembly 2 - Reagent testing panel

3 - Electromagnetic valve assembly 4 - Sample syringe

2.2 Hydraulics System

2.2.1 Schematic Diagram of the Hydraulic System

2.2.2 Hydraulics Diagram for the Overall Unit

2.2.3 WBC/HGB Measurement Channel

Channel Principles and a Description of Their Functions

2.2.4 RBC/PLT Measurement Module

Channel Principles and a Description of Their Functions

 Graph information: RBC histogram and PLT histogram

2.2.5 Sampling and Blood-dispensing Module

2.2.6 Power Supply and Waste Discharge Module

2.2.7 Status Monitoring Module

2.2.8 Hydraulic Components

List of Hydraulic Components

No. Componen Illustration Material No. Function Description Remarks

1 Sample 56.01.0163A Collects blood samples in the None

2 Swab 56.01.0063A Cleans the outer and inner None

3 Two-way 24.13.0010A On-off switch for fluid control None