Problem Set 5

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Biochemistry: Biomolecules, Methods, and Mechanisms

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Course / Unit: Buffers and Amino Acids / Problem Set: Buffers and Amino Acids

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Wrapping Up Buffers and Lysozyme Characterization


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Problem Set due May 4, 2021 23:00 HKT Past due


Follow-up Experiments
0.0/1.0 point (graded)
You and Cesar measure and record your lysozyme activity at different in triplicate. You graph your current results.

What are some follow-up buffers and pH conditions you could test to further investigate the optimal range of your enzyme? Select all
that apply.

Test the enzyme activity of your lysozyme in an acetic acid buffer at .

Test the enzyme activity of your lysozyme in an acetic acid buffer at .

Test the enzyme activity of your lysozyme in a phosphate buffer at .

Test the enzyme activity of your lysozyme in a phosphate buffer at .

Submit You have used 0 of 2 attempts


Identifying Your Lysozyme
0.0/1.0 point (graded)
Your group makes the appropriate follow-up buffers and determines the optimal range of your lysozyme. You carefully record your
experimental results and cross-check them with Yonchien and Cesar. Eva, the TA of your specific lab section, walks by as your group
wraps up. You stop her and ask which lysozyme your group was assigned. She hands you a piece of paper with a list of the various
lysozymes assigned to students so you can see for yourself.

Lysozymes and their Optimal Conditions

Lysozyme Optimal temperature (℃) Optimal range

papaya
clam

hen egg white


Japanese flounder g-type
Japanese flounder c-type

European sea bass

Without the results of your follow-up experiments, which of the listed lysozymes could have been your group’s assigned lysozyme?
Select all that apply.

papaya

Japanese flounder g-type

Japanese flounder c-type

hen egg white

Submit You have used 0 of 2 attempts

Identifying Your Lysozyme Continued


0.0/1.0 point (graded)
The complete results of your follow-up experiments are given.

What was your group’s assigned lysozyme? Select the best answer.

Japanese flounder c-type


hen egg white

Japanese flounder g-type

papaya

Submit You have used 0 of 1 attempt

Thoughts After Lab


You leave project lab, pleased with your group’s success. You meet up with Dorothy, who is waiting for you outside in the hall. You
share your reflections on the lab. You both enjoy the attention to detail that biochemistry requires. You tell her you had a great time
working with Yonchien and Cesar, and recommend either of them as future lab partners.

Discussion
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Topic: Problem Set: Buffers and Amino Acids / Wrapping Up Buffers and Lysozyme
Characterization

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! Identifying your lysozyme: answers look odd


2
Not sure if I follow which responses are correct and the answer seems inconsistent with the following question. I will look this u…

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