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    Agar To Grain

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    Sorath Meza
    This document summarizes one person's process for inoculating rye berries with agar to create grain spawn. Key points: - An agar wedge from a petri dish was used to inoculate 6 jars of rye berries. - By day 3, the mycelium had covered the agar wedge and started colonizing the grains. - On day 7, the jars were shaken to further distribute the mycelium throughout the grains. - As of day 13, the mycelium was fully colonizing the grains and the jars were shaken again. - The next update will be when colonization is fully complete, at which point the document discusses spawning to bulk substrate.

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    © All Rights Reserved
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    Agar To Grain

    Uploaded by

    Sorath Meza
    67 views3 pages
    This document summarizes one person's process for inoculating rye berries with agar to create grain spawn. Key points: - An agar wedge from a petri dish was used to inoculate 6 jars of rye berries. - By day 3, the mycelium had covered the agar wedge and started colonizing the grains. - On day 7, the jars were shaken to further distribute the mycelium throughout the grains. - As of day 13, the mycelium was fully colonizing the grains and the jars were shaken again. - The next update will be when colonization is fully complete, at which point the document discusses spawning to bulk substrate.

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    Fórmula de agar

    Original Title

    Agar to Grain

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    This document summarizes one person's process for inoculating rye berries with agar to create grain spawn. Key points: - An agar wedge from a petri dish was used to inoculate 6 jars of rye berries. - By day 3, the mycelium had covered the agar wedge and started colonizing the grains. - On day 7, the jars were shaken to further distribute the mycelium throughout the grains. - As of day 13, the mycelium was fully colonizing the grains and the jars were shaken again. - The next update will be when colonization is fully complete, at which point the document discusses spawning to bulk substrate.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    67 views3 pages

    Agar To Grain

    Uploaded by

    Sorath Meza
    This document summarizes one person's process for inoculating rye berries with agar to create grain spawn. Key points: - An agar wedge from a petri dish was used to inoculate 6 jars of rye berries. - By day 3, the mycelium had covered the agar wedge and started colonizing the grains. - On day 7, the jars were shaken to further distribute the mycelium throughout the grains. - As of day 13, the mycelium was fully colonizing the grains and the jars were shaken again. - The next update will be when colonization is fully complete, at which point the document discusses spawning to bulk substrate.

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    © All Rights Reserved
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    You are on page 1of 3

    SpitballJedi Agar to Grain (A2G)

    Ancient
    Astronaut
    08/05/14 09:42 This is part of my series "The Basics"
    PM
    This is my first grow since loosing all my cultures and spores due to flooding about 9
    months ago.

    Once you have your grains prepared, you need to inoculate the grains. Once 100%
    colonized, it becomes "Grain Spawn". Grain spawn, aka spawn, is usually mixed with a
    bulk substrate, allowed to colonize again, and then fruited. This process is often refered to
    as "Spawning To Bulk". In my case, I will be spawning to bulk in a monotub.

    There are different ways and grains for making spawn. Many people, especially beginners,
    use a syringe with spores (aka MS or Multispore).

    In this write-up, I used a colonized agar wedge to inoculate Rye Berries. I don't want to
    spend too much time explaining agar because I really just wanted to show what what spawn
    looks.

    Here's what I did to make this grain spawn:

    On 2014-07-19, I began with a single drop of MS solution on a single agar plate. I've gone
    through about 50ish plates so far trying to get limited, clean, and healthy looking mycelium.

    I have it narrowed down to 12 plates I will be growing out over time. Each plate is 4
    transfers from MS. Not an isolate, but "limited" genetics.

    I didn't get a pic of the agar plate because of condensation.

    Here is the first A2G, from the new beginning, using my Rye Berry Tek.

    The agar recipe is:


    10g agar agar
    8g LME
    500ml water.

    Be careful when dropping in your wedge. These things really stick the glass and are almost
    imposable to get unstuck. You don't have to shake the jar right away. What I do is, once the
    lid is closed, I roll it around a little to get some grains to cover it. Then, I just let it be. It
    doesn't really matter if the agar sticks to the glass as long as it's covered with some grain.

    Day 1

    Drop agar wedges, from a single petri, in to 6 jars


    Day 3

    The mycelium has mostly covered the agar wedges and is colonizing the grains.

    Day 5

    Mycelium is definantly on the grains. Starting to see rhyzomorphic mycelium similar to


    what was on the agar plate.

    I'll probably give them a good shake in a couple of days. I will post an update at shake time.

    Day 7

    Before and after shaking. The clusters around the agar are bigger than they appear in the
    pic. They broke up really easy with some shaking. No banging required. Got it all good and
    spread out.

    Day 12
    Sidenote: I may make an adjustment to the dry time on my grain prep. I just wanted to
    mention this tek is meant to be beginner friendly and by default errs on the dry side. A
    slightly wetter grain would colonize faster, but, it's easy to get too wet, so adjust with
    caution.

    Day 13
    I went ahead and gave the jars another shake. They broke up very easily with a couple little
    bounces on my bike tire and then some shaking.

    My next update will be at 100% colonization.

    I'll update as I go.

    Coming soon: Making Bulk Substrate and Spawning to bulk

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