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Stem Cell

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Stem Cells and Development

Embryonic Stem Cell Ex Vivo


ESC
ESC Markers: CD9, CD24, CD29, Somatic Stem Cells
CD49f, CD117, CD133, CD324, S Self Renewal Disease Modeling
Zygote 4 Cell Stage 8 Cell Stage Blastocyst SSEA-1, SSEA-3, SSEA-4, G1 G2 iPS Cell Induction
Tra-1-60, Tra-1-81 M
Modified mRNAs
Hematopoietic Stem Cell Niche ESC Chemical Induction Gene Therapy
SRC, MYB, NANOG,
Sinusoid Oct-4, SOX2 Transcription Factors
LIF Cell Cycle
BMP-4 Oct-4 MYC Induced Pluripotent Drug Discovery
Entry Terminal
SOX2 Klf4 Stem Cells
IL-7 Differentiation Differentiation
EPO Maintenance MAPK S
Notch
Ligands
Arteriole Cyclins
Rb
G1 G2
M Neural Stem Cell Niche
Endothelial Jag1 Osteoblasts bFGF (FGF2) Marginal zone Cajal-Retzius Cells Niche
Activins
Cells Noggin
LIF
Diffusable
HSC Expansion Cortical plate Signals
Wnt CP Neurons

Differentiation
SCF
Primitive Quiescence:
Subplate
CXCL12 Streak NT-3
Markers: GFAP, intermediate
Neurofilament, zone SP Neurons
Megakaryocyte Activin A Self-
Activin A Olig2
CXCL4 bFGF (FGF2)
bFGF (FGF2) Subventricular Intermediate Renewal:
TGF-β1 Wnt
Wnt-3a zone Progenitor Cells
HSC Maintenance TPO BMP-4
Nodal
VEGF
Ectoderm SHH
Ventricular Radial Glial EGF
Mesoderm zone Progenitor Cells FGF

HSC Markers IL-3


Human: CD34, CD90, CD135, Lin- HSC
IL-6
Hemangioblast
Endoderm Ectoderm
Mouse: CD34-, CD48-, SCF
CD117, CD150 (SLAM), Lateral
Lin-, Sca-1 Hemangioblast Markers Intermediate Paraxial
Plate
Human: CD133 Mesoderm Mesoderm
MPP Markers Mesoderm Definitive
Mouse: Brachyury, Flt3
Human: Endoderm Markers: CD15, CD29,
Markers: CXCR4, Outer
CD34, CD38-, MPP CMP MEP MEP Markers CD49f, CD133, Nestin,
FOXA2, GATA4,
CD90, Lin- Human: CD34, CD71, CD110 Ectoderm Neural Tube
Pax6, SOX2
GATA6, SOX17
Mouse: Mouse: CD34-, CD41, CD105, CD150
CD34, CD117,
CD150-, CMP Markers
Lin-, Sca-1 Human: CD34, Kidney
Neural Crest
CD38, CD45RA-, CD135 Neural Stem Cell
Platelets Gut Tube
Mouse: CD34, CD117, Wnt
CLP Markers CD135, Sca-1- Retinoic Acid
Human: CD10, CD34,
FOXD3
CD45RA, CD135, Gonads Skeletal Muscle SOX10
Lin- CLP GMP Markers Red Blood Cells
Megakaryocyte
Mouse: CD117low, Human: CD34, CD38,
CD127, CD135, Lin- CD115, CD116, CD123 GMP Retina
Eyes
Mouse: CD16/32, CD34, Neuron
CD117, Sca-1-
Neutrophil
Basophil Adipocytes Dentin

Endothelial Cells
Lung
Glial Progenitor

Mouth Epithelium Spinal Cord


Eosinophil Pancreas

Mature B Cell Innate T Cell Progenitor Melanocytes


Lymphoid Bone
Smooth Muscle Thymus
Cell Monocyte
Olfactory Brain Astrocyte
Thyroid Epithelium
Oligodendrocyte
Cartilage Peripheral Neurons
Plasma Cell CD4+ T Helper Cell CD8+ Cytotoxic T Cell Dendritic Cell Macrophage Cardiomyocytes Gut

Contact BioLegend

biolegend.com Interactive Poster: biolegend.com/stemcellposter US & Canada Toll-Free: 1.877.246.5343 (877-BIOLEGEND) US Headquarters:
International: 1.858.768.5800
San Diego, CA 92121
We would like to thank Dr. Stephen Dalton, University of Georgia for his contributions to this poster. Fax: 1.877.455.9587
email: cs@biolegend.com, tech@biolegend.com 04-0013-02

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