UV Visible Spectros
UV Visible Spectros
UV Visible Spectros
MSc II SEM
INSTRUMENTATION ASSIGNMENT
ELECTRONIC TRANSITION
According to molecular orbital theory, the excitation of a molecule by the absorption of
radiation in the UV-visible regions involves promotion of its electrons from a bonding, or non
bonding (n)orbital to an antibonding orbital.
■o.o* transition ■n-o* transition
■ π-π* transition ■n-π* transition
ISHA RAJPUT (19)
MSc II SEM
INSTRUMENTATION ASSIGNMENT
ABSORPTION AND INTENSITY SHIFT
INSTRUMENTATION
COMPONENTS:
1. Radiation Source
# A stable continuous radiation is required.
ISHA RAJPUT (19)
MSc II SEM
INSTRUMENTATION ASSIGNMENT
# It must be of the sufficient intensity for the transmitted energy to be detected at the end of the optical path.
2. Monochromator
# It is a device that can we prymatic radiation into component wavelengths.
# The monochromator unit consists of:
4. Detector :-
Device which converts light energy into electrical signals, that are displayed on readout
devices.
The transmitted radiation falls on the detector which determines the intensity of radiation
absorbed by sample.
Requirements of an ideal detector:
✓It should give quantitative response.
✓ should have high sensitivity and low noise level.
✓It should have a short response time.
TYPES OF DETECTORS
ISHA RAJPUT (19)
MSc II SEM
INSTRUMENTATION ASSIGNMENT
1. Barrier layer cell/Photovoltaic cell
2. Phototubes/Photo emissive cell
3. Photomultiplier tube
ADVANTAGES
DISADVANTAGES
Chromophore dependent
Liquid samples only.
Stray light headache
Specificity at low wavelength