Service manual

1 Rev. 3
 Manufacturer:
DYNEX TECHNOLOGIES, spol. s r.o.
Vodičkova 971/41
110 00 Praha
Czech Republic
Tel: +420 220 303 600
Fax: +420 224 320 133
E-mail: info@dynex.cz
www.dynex.cz

Copyright

Copyright © Revised 2017 DYNEX TECHNOLOGIES, spol. s r.o.

All rights reserved. No part of this document can be copied, transcribed or altered in any way,
electronically or mechanically, including photocopying and recording for any purpose other than
the use by the buyer without a prior written permission of DYNEX TECHNOLOGIES, spol. s r.o.

Restrictions and Duties

The information contained in this document can be modified or adjusted and are not binding for
DYNEX TECHNOLOGIES, spol. s r.o. The changes will be made in the new edition of this
publication.
DYNEX TECHNOLOGIES, spol. s r.o. do not assumes any liability for the use of harmlessness of
the devices and software not supplied directly by the manufacturer or authorized distributor.

2 Rev. 3
Revision table

Revision Date Changes

0 15.1.2013 First official edition

1 27.9.2016 Cleaning bowl maintenance, deleting
2 27.4.2017 Reconstruction of manual contents
3 21.8.2017 Changes for instruments of Variant 3

3 Rev. 3
Obsah/Content
1 General description _________________________________________________________ 9
2 Disposition ________________________________________________________________ 9
2.1 Front View ____________________________________________________________ 9
2.2 Rear View ____________________________________________________________ 10
2.3 Workspace ___________________________________________________________ 11
2.3.1 Working arm ______________________________________________________________ 11
2.3.2 Sample tubes rack__________________________________________________________ 13
2.3.3 Strips tray holder __________________________________________________________ 13
2.4 System solution bottle holder _____________________________________________ 14
2.5 Waste bottle __________________________________________________________ 14
2.6 Calibration cuvette _____________________________________________________ 15
2.7 Space under the front cover ______________________________________________ 15
2.8 Space under rear cover _________________________________________________ 16
2.9 Meaning of LEDs control ________________________________________________ 18
2.9.1 Mainboard - control LED_____________________________________________________ 18
2.9.2 Armboard - control LED _____________________________________________________ 19
3 DynLab software __________________________________________________________ 19
3.1 Installation, startup, settings ______________________________________________ 19
3.2 Manual control of individual instrument components ___________________________ 20
3.3 Setting the instrument parameters and storing in FLASH memory ________________ 22
3.4 Updating firmware in processor memory ____________________________________ 23
3.5 Saving D-code segments ________________________________________________ 24
3.5.1 Saving segments to FLASH memory ____________________________________________ 24
3.5.2 Saving segments to RAM memory _____________________________________________ 25
3.6 Run execution of segments or individual D-code instructions ____________________ 25
3.6.1 Running of the segment in DynLab SW _________________________________________ 25
3.6.2 Running of the segment stored in instrument memory _____________________________ 27
3.7 Running an individual D-code instructions ___________________________________ 27
4 Import segments with Dynablot Automatic application _____________________________ 27
5 Setting procedures_________________________________________________________ 28
5.1 Position adjustment of home position sensors ________________________________ 28
5.1.1 Home sensor of X motion ____________________________________________________ 29
5.1.2 Home sensor of Y motion ____________________________________________________ 29
5.1.3 Home sensor of Z motion ____________________________________________________ 29
5.1.4 Home senzor syringe _______________________________________________________ 30
5.1.5 Home sensor of rocking _____________________________________________________ 30
5.1.6 Aspiration arm ____________________________________________________________ 31
5.2 Setting aspiration and filling arms of reagents ________________________________ 31
5.3 Setting mechanical positions of needle _____________________________________ 32
5.4 Vacuum in the waste bottle adjustment _____________________________________ 34
5.4.1 The maximum vacuum value adjustment _______________________________________ 34

4 Rev. 3
 5.4.2 The vacuum in waste bottle sensor adjustment __________________________________ 34
5.5 The camera system adjustment ___________________________________________ 35
5.5.1 The camera control software _________________________________________________ 35
5.5.2 Mechanical position of camera adjustment ______________________________________ 38
5.5.3 Area of Interest (AOI) adjustment _____________________________________________ 39
5.5.4 Illumination, camera aperture and focus adjustment ______________________________ 40
5.6 Setting barcode reader __________________________________________________ 44
5.7 Coordinate settings using i-parameters _____________________________________ 46
5.7.1 Setting i-parameter of position X above the priming cuvette ________________________ 46
5.7.2 Setting i-parameter of rocking control __________________________________________ 46
5.7.3 Setting i-parameter coordinates and constants (Instrument coordinates and constants) __ 47
6 Instrument maintenance ____________________________________________________ 55
6.1 The instrument checking and preventive maintenance operations list ______________ 55
6.2 Disassembly the instrument covers ________________________________________ 56
6.2.1 Front cover - disassembly ____________________________________________________ 56
6.2.2 Rear cover - disassembly ____________________________________________________ 56
6.2.3 Workspace cover - disassembly _______________________________________________ 57
6.3 Replacement of pump cassettes and reagent tubes ___________________________ 59
6.3.1 Pump cassette - replacement _________________________________________________ 59
6.3.2 Filling tubes - replacement ___________________________________________________ 60
6.3.3 Tubes for reagent bottles - replacement ________________________________________ 63
6.4 Exchange of aspiration tubes _____________________________________________ 64
6.5 Replacing the vacuum system tubing _______________________________________ 67
6.6 Replacing the pipette system components ___________________________________ 69
6.6.1 Status test and replacement of the system solution pump __________________________ 69
6.6.2 Replacing the system solution tubes ___________________________________________ 70
6.6.3 Replacement of needle and tubes _____________________________________________ 72
6.7 Shift maintenance ______________________________________________________ 74
6.7.1 X - shift __________________________________________________________________ 74
6.7.2 Y - shift __________________________________________________________________ 75
6.7.3 Z – shift __________________________________________________________________ 76
6.7.4 Rocking __________________________________________________________________ 76
6.8 Maintenance of exhaust silencer and reverse throttle valve ______________________ 77
6.9 Maintenance of barcode reader ___________________________________________ 78
6.10 Camera system maintenance _____________________________________________ 79
6.11 Dismantling the syringe _________________________________________________ 80
6.12 Dismantling of needle cleaning cuvette _____________________________________ 81
6.13 Dismantling the peristaltic pump motor _____________________________________ 82
6.14 Dismantling pump of vacuum in waste bottle _________________________________ 83
6.15 Checking of the instrument functionality _____________________________________ 84
7 History of instruments running- log files _________________________________________ 84
8 Principles of operation ______________________________________________________ 85
8.1 Communication________________________________________________________ 85

5 Rev. 3
 8.2 Hydraulic scheme of reagent filling system __________________________________ 85
8.3 Hydraulic scheme of exhaust system _______________________________________ 86
8.4 Hydraulic scheme of pipetting system ______________________________________ 86
8.5 Fluid level detection with pipettor needle ____________________________________ 87
8.6 Automatic calibration of peristaltic pumps ___________________________________ 88
8.7 Level detection in system solution bottle ____________________________________ 89
8.8 Barcode reader ________________________________________________________ 90
8.9 Capturing strip images __________________________________________________ 91
9 Appendix ________________________________________________________________ 92
9.1 D-code segments ______________________________________________________ 92
9.1.1 001 selftest RUN.Dcod ______________________________________________________ 92
9.1.2 020 System voiding RUN ____________________________________________________ 92
9.2 Assay listing of Instrument check __________________________________________ 93
9.3 Preventive maintenance protokol __________________________________________ 94
9.4 Hydraulic diagram______________________________________________________ 95
9.5 Mainboard connector map _______________________________________________ 96
9.6 Armboard connector map ________________________________________________ 97

6 Rev. 3
Warning, Cautions and Notes

The following types of notices are used in this publication and highlight important information or
warn the user of a potentially dangerous situation:

NOTE
Gives helpful informations.

CAUTION
Indicates a possibility of instrument damage or data loss if instructions are
not followed.

WARNING
Indicates the possibility of severe personal injury, loss of life or equipment
damage if the instructions are not followed.

INSTRUCTION
Actions to be performed.

WARNING
This symbol indicates the possible presence of biologically hazardous
material.
Proper laboratory safety precautions must be observed.

ATTENTION
Negative environmental impacts associated with the treatment of waste.
Do not handle electrical and electronic equipment as unsorted municipal
waste.
Collect waste electrical and electronic equipment separately.

WARNING
Risk of fire and explosion!
Several disinfection products can be flammable and when improperly
handled can lead to explosions. Proper laboratory safety precautions must
be observed.

WARNING
Chemical hazardous and biohazardous waste can be associated with the
waste material from the process run on DBH.
Treat these substances and disposables, such as trays, system liquid, etc.
in accordance with good laboratory practice guidelines.
Get inquire about appropriate collecting points and approved methods of
disposal in your country, state or region.

7 Rev. 3
Use symbols

Manufacturer

Date of manufacture

In vitro diagnostics medical device

Catalogue number

Serial number

Indicates the possible presence of biologically hazardous material

Conformité Européenne

Toxic

Hot surface

Use by

Single use

USB

Consult service manual

Warning

Instruction

8 Rev. 3
1 General description
Dynablot Automatic is a computer-controlled instrument designed to automatically perform stripping
assay steps. Automation includes these features:
Handling of reagents filling and aspiration reagents from the reaction wells of the plate
Handling with the sample reading the sample ID from the bar code on the primary tube,
transfering the sample from the primary tube to the reaction well
Incubation time-controlled incubation of the strips in the reaction wells, mixing of
the reagents by rocking the strip plate
Drying drying the processed strips using the airflow and the tray holder heating
Capturing images capturing images of the processed strips using a camera system
Data manipulation saving protocols and strip images in an internal database of device
control SW, ability to collaborate with an external evaluation SW in the
form of importing and exporting protocols
The device control SW allows the user to perform and monitor:
 preparation and running of the protocol
 routine maintenance of the instrument
 creating and editing assays and tests
 protocol history and maintenance history
 setting the instruments’s operating parameters

2 Disposition
2.1 Front View

4
1

5
2

Picture 1

1 - power-on lamp 4 - peristaltic pump of the system solution

2 - peristaltic pumps for the reagents 5 - reagent bottle pad
3 - workspace cover

9 Rev. 3
 2.2 Rear View

3 6

4
7
5
8

10

11 13

12

Picture 2

1- fans of the workspace 9 - pinch valve of the aspiration cleaning

2- spring struts of the cover needle cuvette
3- vacuum tube outlet for the waste bottle 10 - pinch valve of the strip wells aspiration
4- vacuum pump exhaust arm
5- USB device control connector 11 connector of the waste bottle liquid level
6- USB camera connector sensors
7- manufacturing label 12 - connector of the level sensors in the
8- power supply cable connection with system solution bottle holder
integrated switch and fuse holder 13 - opened holder with the fuses

10 Rev. 3
 2.3 Workspace

6
2

3 7

4
8

Picture 3

1- working arm 5- label for the camera checking

2- pipette module 6- right fan of the workspace
3- filling and aspirating arms of the reagents 7- space for the sample tube rack
4- fan of the strip drying 8- the strips tray holder

2.3.1 Working arm

1 4

2 5

Picture 4

1 – dispensing arm for the reagents 4 – needle cleaning cuvette

2 – the reagent priming cuvette 5 – aspiration arm
3 – pipette needle

11 Rev. 3
 1

2
View after removing the cover
of the pipette module
3

1 – stepper motor of the Z motion

4
2 – tube between the syringe and the needle
3 – home sensor of the Z motion
5
4 – pipette needle
5 – level detection circuit cable
6
6 – bolt of the Z motion
7 – stepper motor of the Y motion
7
8 – toothed rod of the Y motion
9 – home sensor of the Y motion
8
10 – sensor of the position of the
aspiration arm
9

10

Picture 5

View after removing the cover of the working arm

1 5

6
2

7
3
8

4
9

Picture 6

1 – camera with the objective 6 – LED lighting 2

2 – three-way valve 7 – mirror for the camera
3 – syringe (plunger pump) 8 – mirror for the LED lighting 1
4 – armboard 9 – reagent dispensing arm
5 – LED lighting 1

12 Rev. 3
 1

3
2

Picture 7

1 - right fan of the workspace 3 – disassembled cover of the working arm

2 – fan of the strips drying

2.3.2 Sample tubes rack

1
4
2

3 5

Picture 8

1 – positions for the control tubes A, B 4 – positions for the control tubes C, D
2 – control bar code for the rear row 5 – positions for the front row of the tubes
3 – positions for the rear row of the tubes

2.3.3 Strips tray holder

3
1

5 6

2
4

Picture 9

1 – pulley with the anti slip o-ring 4 – rotary hinge of the frame
2 – eccentric drive wheel 5 - heating foil with thermostat - limiter
3 – frame to place the strips tray 6 - temperature probe for the regulator

13 Rev. 3
 2.4 System solution bottle holder

1 6 7

Picture 10

1 – cap with the suction tube 5 – fitting to connect the system solution
2 – system solution bottle pump
3 – holder 6 – receiver of the level detection system
4 – cable with the level sensor connector 7 – transmitters (LED) of the level detection
system
2.5 Waste bottle

1 5

4
6

Picture 11

1 – cable with the level sensor connector 5 – fitting for the waste tube connecting
2 – fitting for the vacuum tube connecting 6 – tube under the waste hose fitting
3 – fitting for the calibration cuvette 7 – float level "Error”
connecting 8 – float level “Warning”
4 – waste bottle

14 Rev. 3
 2.6 Calibration cuvette

1 2

Picture 12

1 – tube with the connector for connection to 3 – hole for the level measurement by the
the waste bottle needle
2 – filling hole 4 – vista into the calibration space

2.7 Space under the front cover

1 1

2 3 4 5 6 7 8 9 10

Picture 13
3 11 12 13

1 – pump motors covers 9 – encoder of the X motion cover

2 – stepper motor of the X motion 10 – connectors of the rocking motor with its
3 – pumps LED indicators boards sensor and the heating foil with its
4 – reagent pump motors temperature probe connection
5 – pump motor’s power filters 11 – vacuum in the waste bottle sensor
6 – vacuum pump 12 – vacuum in the waste bottle regulator
7 – reverse throttle valve 13 – earthing cabel of the cover
8 – power filter of the vacuum motor pump

15 Rev. 3
 1 4

2
5

Picture 14

1 – removed front cover 6 – stepper motor and the sensor connection

2 – eccentric drive wheel cable
3 – rocking home sensor 7 - the heating foil and the temperature probe
4 – faston for the grounding cable cable
5 – rocking stepper motor

2.8 Space under rear cover

Picture 15

16 Rev. 3
 1 2 3 4 5 6

7 8 9

Picture 16

1 – power supply 6 – solenoid of pinch valve for the wells

2 – barcode for barcode reader checking aspiration arm
3 – USB camera connector board 7 – trough for the cabling
4 – mainboard 8 – power chain with cabling and tubes to the
5 – solenoid of pinch valve for cleaning working arm
needle cuvette aspiration 9 – exhaust silencer of the vaccum pump

1 2 3

Picture 17

1 – rail of the X motion 4 – barcode reader on the base of the

2 – pulley and belt of the X motion stepper working arm
motor 5 – rotary mirror for reading the barcode
3 – home sensor of the X motion

17 Rev. 3
 2.9 Meaning of LEDs control
There are green LEDs placed on the electronic boards – Mainboard and Armboard. These LEDs
indicate input and output states. For information on the connectors on the boards and the
components connected to them see 9.5 Mainboard connector map and 9.6 Armboard connector map

2.9.1 Mainboard - control LED

4 5

7 8 9 10 11 12 13 14 15 16 17 18

Picture 18

1 – encoder of the X motion, blinks while 11 – left workspace fan, illuminates when the
moving output is switched on
2 – home sensor of the X motion, illuminates 12 – right workspace fan, illuminates when
when the arm is in the home position the output is switched on
3 – rocking home sensor, illuminates when 13 – system solution pump, illuminates when
the eccentric wheel is on the_sensor the output is switched on
4 – vacuum sensor in the waste bottle, pair of 14 – power supply of the system solution
LEDs – the upper LED illuminates when holder, illuminates when the output is
the under pressure is created switched on
5 – workspace cover sensor, illuminates 15 – level detection of the system solution
when the cover is closed bottle – warning, illuminates in the
6 – power voltage presence of the solution
7 – heating foil 16 – level detection of the system solution -
8 – pinch valve of the needle cleaning cuvette error, illuminates in the presence of the
aspiration, illuminates when the output is solution
switched on 17 – level detection of the waste bottle -
9 - pinch valve of the strip wells aspiration warning, illuminates in the absence of the
arm, illuminates when the output is solution
switched on 18 – level detection in the waste bottle –
10 – vacuum pump, illuminates when the error, illuminates in the absence of the
output is switched on solution

18 Rev. 3
 2.9.2 Armboard - control LED

1 2 3 4 5

Picture 19

1 – home sensor of the Y motion, illuminates 4 – position sensor of the aspiration arm,
when the arm is in the home position illuminates in the upper position of the
2 - home sensor of the Z motion, illuminates arm
when the arm is in the home position 5 – barcode reader signal, flashes when the
3 - home sensor syringe, illuminates at the code is detected
home position of the syringe plunger

3 DynLab software
DynLab is a service, widely-used PC software for electronic control systems used in Dynex
equipment.
WARNING
Use only the DynLab software features described in this chapter if you fully
understand them. Incorrect use may cause mechanical damage or
decommissioning due to incorrect firmware or parameter settings.

The software has these basic functions

 Manually control of each instrument components
 Settings the device parameters (i-parameters) and store them into FLASH processor memory
 Updating the firmware in the processor memory (for example new version of the basic
firmware)
 Creating and storing D-code segments (sub-programs used by the firmware to control the
device) into processor memory
 Running segments or individual D-code instructions

3.1 Installation, startup, settings

Installing DynLab software is done only by coping the directory with the appropriate files to your PC.

The versions of these files differ for different devices:

Constants.ini – can also have a different name, e.g. DBAConstants.ini. It describes a layout and
names of the motors, system inputs and outputs.
FlashParams.ipar – always has this name. Contains a default list and basic i-parameters values. .

19 Rev. 3
DynLab runs with a file DynLab.exe
After the first run, some software parameters must be set. The window Options opens from the Setup
/ Options or by an icon .

Picture 20

BatchFlashFiles – directory path for D-code segment files saving. They will be stored into FLASH
instrument memory.
BatchRAMFiles – directory path for D-code segment files saving. They will be stored in RAM
instrument memory. These are temporary segments used to check or service your device.
InstrumentElements - file name, e.g. Constants.ini with the layout of the motors, system inputs and
outputs

The connection status of the instrument is displayed in the lower bar of the DynLab window. It is
displayed before or after disconnection

or .
If the instrument is connected via USB to the PC, the firmware version of the mainboard is listed on
the right side of the bar.

3.2 Manual control of individual instrument components

Manual control of the instrument components can be used to diagnose device defects or to search
for movements coordinates while setting instrument parameters. The window Components control
opens from the Instrument / Components control or by an icon . The left part of the window is
used for control.

20 Rev. 3
 1

Picture 21

Stepper motors motion control (1)

There are the names of the individual stepper motors in the rows :
X Motor - travel of the working arm right (+) and left (-)
Y Motor - travel of the pipette module forward (+) and backward (-)
Z Motor - travel of the pipette needle down (+) and up (-)
Plun.pump - travel of the piston syringe for filling (+) and dispensing (-)
Rocking Motor – rotation of the excentric wheel for rocking of the plate holder
The motors are operated with the buttons

- getting to the starting position (it starts movement towards the home sensor and stops
when the signal is activated)

- relative shift of the coordinate value listed in the column JOG

- shift to the absolute value of the coordinate listed in the column Absolute (on the left from
button.The current position coordinate is displayed in the column Absolute (on the right).

Control of binary outputs (2)

There are the names of the individual parts of the instrument connected to the outputs in the rows:
Heating - power supply of the heating foil controler
Needle cleaning valve - pinch valve of the needle cleaning cuvette aspiration
Aspiration arm valve - pinch valve of the wells aspiration arm
Aspirating pump - vacuum pump
Fan left - left fan of the workspace
Fan right - right fan of the workspace
System solution pump - peristaltic pump of the system solution
System sol.level supply - supplying the level detection circuits in the system solution bottle
holder
21 Rev. 3
LED 1 - LED 9 - indicating LEDs of the reagent peristaltic and system solution pumps
Pump 1 - Pump 8 - reagent peristaltic pumps
3-way valve - 3-way valve (system solution flows into: Off – the needle cleaning
cuvette, On - the syringe)
Camera light - LED lighting1 and 2 for the camera

The outputs are controlled by buttons:

- turning the ouput ON (depending on the power-on parameters)

- turning the output OFF

Turning on can be parameterized in the fields

Volume ml – if the outputs are provided with volumetric calibration (here outputs Pump 1 - 8), it is
possible to enter the volume in tenths of ml. Then the pump is triggered only in relation to
its calibration constant stored in i-parameters for the time required to pump the specified
volume. When the value 0 is entered, the pump is started continuously.
Ms s min – according to the selected unit, the output is switched only for the specified time. When
the value 0 is entered, the output is started continuously.
Wait.version code – when selecting the switching output option to the specified volume or time, It
blocks the possibility of simultaneously switching another output.

3.3 Setting the instrument parameters and storing in

FLASH memory
The parameters (i-parameters) affecting the firmware are stored in the instrument memory. Some
parameters are the same for all instruments of one type, other may vary slightly for each piece
manufactured.
The left part of the window Components Control is used to edit and save i-parameters. It is opened
by Instrument / Components Control or an icon .

1 2 3

Picture 22

22 Rev. 3
Table description (1)
I-parameters are placed in bookmarks in function groups. There are some links to bookmarks at the
top of the table (2). The list of all bookmarks (4) opens by clicking the arrow (3).
The i-parameter group table contains columns:
Index – the i-parameter register ID. According to this number, the i-parameters in the table are initially
sorted.
Name – the name with the i-parameter function. When you click the column head, the i-parameters
are alphabetically sorted by name. Names that start with a number then create a clear list
of parameters, for example in order of how they are adjusted during the instrument set up.
FLASH Value – i-parameter values read from the connected instrument. Values can be edited in this
column.
File Value - i-parameter values retrieved from a file previously stored on a PC or from the default file
FlashParams.ipar.

Description of buttons (5)

- opens a window to select a previously saved .ipar file. When you open the file, it displays
its values in the column File Values. If the instrument is connected, it also reads the values
from its memory into a column FLASH Value.

- opens a window to select the location and file name of the .ipar file. Subsequently, this file
saves values from the FLASH Value column.

- reads the values from the instrument and saves them to the FLASH Value column. To
create a table structure, the default file is FlashParams.ipar, whose values are displayed in
the File Values column.

- writes the values from the FLASH Value column to the instrument memory. Before saving
there is displayed an optional choice to store values into the file (see Save File button)

- in all tabs, the values of the File Value column are overwritten in the FLASH Value column.

3.4 Updating firmware in processor memory

Mainboard firmware can be updated using DynLab software, via USB. Firmware updates are
released fo this purpose as DBA_X_XX.S19 files.
X_XX means the version number (e.g. DBA_4_38.S19 for version 4.38).

How to update firmware

When the instrument is turned on and connected, the Instrument Memory / Update.Firmware is
selected.

Disconnection of the instrument is shown in the lower bar for a short time.
.
Then the Bootloader is connected and the .S19
file selection window is opened.
23 Rev. 3
Updating process and completion are shown in windows below:

Then the instrument must be turned OFF. The next time when the instrument is turned ON firmware
is now up to date and running.

3.5 Saving D-code segments

3.5.1 Saving segments to FLASH memory

Segments are short subroutines in D-code language. In the cases of update, they are released in
the Segment package – File direcory. The name of this segment package is
DBA_SegmentPack_X_X_X_X .
X_X_X_X means the version number (e.g. DBA_SegmentPack_1_2_0_0 for version 1.2.0.0).

NOTE
The D-code Segment package can also be saved to the instrument using
application Dynablot Automatic for the device control. For this purpose,
packages are released in one special file format (see 4 Import segments with
Dynablot Automatic)

How to save D-code segments

Copy all package files to the directory that is set in the row BatchFlashFiles, window Options.
(e.g. to the directory BatchFlashFiles when set up
C:\Users\DynablotAutomatic\Documents\DynLab\BatchFlashFiles)

Storing segments in the memory starts in the menu: Instrument Memory / Save Batch Into FLASH

memory or by the button in the upper bar and confirm the check query.
A Console window opens in which the segments can be watched. After all segments are successfully
saved, the following is displayed :

Picture 23

24 Rev. 3
 3.5.2 Saving segments to RAM memory

For assembly or service activities, special segments can be temporarily stored in RAM memory.
These segments can be run using DynLAb (see next chapter). After the instrument is turned off, the
stored segments are deleted from the instrument memory.
The procedure for storing segments into RAM memory is similar to FLASH memory. Differences are
in starting the storage and setting the path to the directory where the recording files are stored.
The path settings is in the row BatchRAMFiles of window Options.
It starts up in the Instrument memory / Save Batch Into RAM Memory or by the button in the
upper bar.

3.6 Run execution of segments or individual D-code

instructions
The D-code segment can be run in the DynLab software and according to its instructions, the
individual activities of the Mainboard are progressively executed via USB.
If a segment contains a specific instruction, another segment stored in FLASH or RAM memory can
be started. During the normal operation of the instrument, the segments of the package in FLASH
memory are ran by the Dynablot Automatic application software. By running from DynLab software,
the individual segments can be tested or it is possible to run segments specifically stored in RAM
memory.

3.6.1 Running of the segment in DynLab SW

A segment opening is made from menu File / Open or by the button in the upper bar. A window
with the opened segment file name appears.

Picture 24

You can edit the segment in the left part of the instruction window. However, segment creation is not
the subject of this manual.
In the right part of the window there is a table with the same instruction structure and is intended to
start the segment. To start, you can use these buttons above the table:

25 Rev. 3
 - running the entire segment, the instruction line to be executed is highlighted in green. Segment
run is terminated after END has been executed
- immediate stop of the executed segment

- pause of the segment runnning

- step-by-step instructions. Press the button to execute the instructions on the green line

- starting the run from the current executed line to the line that you clicked on.

If the segment code contains instructions for sending messages (SMS X,X) the windows with
information about SMS are displayed. These messages are used to communicate with the Dynablot
Automatic application SW.

You can close individual message window by pressing the or all windows by button
in the top bar.

Press the button in the top bar to open or close the information box on the sides of the main
window where the communication between the DynLab SW and the instrument in logged.

Picture 25

In the case of a component control or running a segment, it is useful to follow the left lower field. It
records errors that the instrument generates in the event of a malfunction (e.g. a motion error X is
detected in the image).
The list of errors displayed in the field can be saved to a file or clear using the menu that opens by

right-clicking on the area of the field .

26 Rev. 3
Use this button to reset USB communication between the PC and the instrument. It can be used
in case of a problem.

3.6.2 Running of the segment stored in instrument memory

If the segment triggered by the procedure described in the previous chapter contains the instruction
RUN 0, a segment stored in the instrument memory is executed after this instruction has been
executed. Selection of this executed segment is done by the address set in the R421 register. Before
starting a segment from the instrument’s memory, it is also necessary to set some registers to
parametrize the segment’s operation.
For example:
To perform a selftest of the instrument, you must run a segment 1 stored in the memory.
The DynLab SW opens or writes the startup segment:
1 2

Picture 26

In the left part of the table you can overwrite some registry values as described in their rows (1).

After pressing the button (2), the boot segment is stored and its instructions are executed. The
RUN 0 instruction initiates the instrument selftest segment.

3.7 Running an individual D-code instructions

You can send a single D-code to the command line .

Intructions are written to a line and this button sends them to the instrument for execution.

4 Import segments with Dynablot Automatic

application
The segment pack can also be saved to the intrument using the Dynablot Automatic application
software. For this purpose, SegmentPack_A_B_C_D.DcdE files are issued.
The version number consists of four digits. The condition to make the saving is that the digits A and
B of the package are the same as the digits in the version of the Dynablot Automatic application
software. This monitors package and software compatibility.
In order to save the segment package to the instrument there must be logged a user as Administrator
or Service in the application software. (see Dynablot Automatic User guide)
Use the Application administration / Segments import menu to open the window

27 Rev. 3
 .
Press the button to open a dialog to select the segment package

These windows below inform about the progress and completion of the import:

This import is registered in the database of the application and the package version is displayed in
the Application administration / About application.

5 Setting procedures
This chapter describes how to set up your instrument. The subchapters are sorted according to the
order in which the new instrument is being set up.

5.1 Position adjustment of home position sensors

Home sensors work on a magnetic principle. The sensor is located on a small printed circuit board,
the magnet is on the moving part. As the magnet approaches the sensor, a signal is sent to the
control unit. The mechanical adjustment is to fix the printed circuit board with the sensor so that the
signal is sent in the desired position of the monitored moving part. The adjustment is performed
when the instrument is switched on without selftesting. In this state, movable parts can be moved
manually and the sensor signal can be monitored by LEDs on the electronics boards.

28 Rev. 3
 5.1.1 Home sensor of X motion
The location of the sensor, Picture 17 position 3. Signal indication is on the Mainboard, Picture 18
position 2.
With the correct sensor position, the fastening screws are approximately halfway through the oval
hole on the board. The horizontal position of the board must be kept up.

The LED lights up when the arm moves from the right to
the left when the tube of the aspiration arm is on or near
the edge of the cuvette.

Picture 27

5.1.2 Home sensor of Y motion

The location of the sensor, Picture 5 position 3. Signal indication is on the Armboard, Picture 19
position 1.

The position of the sensor is not critical. Set the left

extreme position.

Picture 28

5.1.3 Home sensor of Z motion

The location of the sensor, Picture 5 position 9. Signal indication is on the Armboard, Picture 19
position 2.

The LED is lit when moving from the bottom up and when the gap between the engine block and
the needle block is L1 = 1.5 to 2 mm.
After the movement Z moves to the 0 coordinate, the gap is L2 = 0.5 mm. There must be no block
collisions!

29 Rev. 3
 L1

L2

Picture 29

When looking for the position L1 then Z

shift motion can be done manually by
rotating of the bolt.

Obr. 30

Checking the movement to the coordinate 0 can be done by DynLab SW:

First use the instruction MMC 3 1 1 to set the movement speed (see 3.7 Running an individual D-
code). Next use the relative movement (-200) to exit the starting position, then the movement to
Home position, then the relative movement (-200) to exit the starting position again and then the
absolute movement to coordinate 0 (see 3.2 Manual control of individual instrument components).

5.1.4 Home senzor syringe

The home sensor of the original piston syringe position is an integral part of this component and
does not need to be set.

5.1.5 Home sensor of rocking

The location of the sensor, Picture 14 position 3. Signal indication is on main board, Picture 18
position 3.

30 Rev. 3
Precise sensor position adjustment is not
required. The LED is on when the eccentric
wheel is in the upper position.

Picture 31

5.1.6 Aspiration arm

The location of the sensor, Picture 5 position 10. Signal indication is on the Armboard, Picture 19
position 4.
The aspiration arm does not have ist own drive. Y shift is used to control it. The aspiration arm
sensor is used only to indicate that the arm is in the upper position by means of a the spring action.

The position of the sensor is set so that

the LED in the upper position of the
arm lights up and goes out by pushing
the arm about L = 2mm.

Picture 32

5.2 Setting aspiration and filling arms of reagents

The arms are adjusted so that their position corresponds to the spacing between the adjacent strip
wells in the tray inserted in the holder. The holder is in the top position when setting up.

The position of the aspiration arm is set first.

In the correct position, the aspiration tube touches the bottom of the well at the point of its rear corner
to achieve as complete aspiration of the well content as possible.

31 Rev. 3
 1

Picture 33

Once the fixing screw (1) is released, the arm assembly together with the sensor can be moved to
the correct position. Then the screw is secured again.
Subsequently, the mutual position of the filling arm and the aspiration arm is adjusted.
The aspiration arm tube is places in the center of the well plate by moving the entire working arm.
The filling arm is adjusted so that the ends of the tubes are above the center of the adjacent well on
the left.

Picture 34

Once the fixing screw (1) is released, the arm can be moved to the correct position. Then the screw
is secured again.

5.3 Setting mechanical positions of needle

The mechanical setting of the needle position is influenced by the overall position of the working
arm. To fine-tighten the needle position, use 4 screws on the needle holder.

32 Rev. 3
 1 2

Picture 35

After the two fastening screws (1) are slightly released, the needle parallelism with the tube rack
(Picture 36 on the left) and the needle position in the center of the hole in the bottom of the cleaning
cuvette (Picture 36 on the right) can be adjusted by manipulating the four adjustment screws (2).
Then tighten the fixing screws carefully and check the position again.

Picture 36

33 Rev. 3
 5.4 Vacuum in the waste bottle adjustment

To set the vacuum level, a tube with a connector

can be used to connect the pressure gauge to the
waste bottle, which is disconnected from the
cuvette for calibration of the pumps (2.6 Calibration
cuvette).

Picture 37

5.4.1 The maximum vacuum value adjustment

The maximum vacuum value in the waste bottle is set by the vacuum regulator (see Picture 13
position 12).
Before setting, make sure that the waste tubes are properly inserted in the pinch valves (see Picture
2 positions 9 and 10) and the waste bottle lid is tightly tightened.
Switch the instrument ON and using the DynLab SW start the vacuum pump (see Picture 21 output
8 Aspirating pump). The vacuum value must be stabilize at about 200 mBar, at least at 160 mBar.

2
1

Picture 38

Before manipulation with the adjusting screw (2), it is necessary to release the fixing nut (1) and
tighten it after adjustment. By tightening the adjustment screw, the negative pressure increases.
If the vacuum is stabilized at a higher value than needed, by enabling the adjusting screw the vacuum
will not decrease immediately as this prevents the non-return valve. If pressure is to be lowered, it is
necessary first to loosen the adjusting screw, then lower the vacuum in the bottle by releasing its lid.
After the lid is tightened, the vacuum will stabilize after a while.

5.4.2 The vacuum in waste bottle sensor adjustment

Sensor adjustment is to set the vacuum value at which contacts are switched (see picture 13 position
11).
Switching the contacts can be monitored by the control LEDs on the mainboard (see PicturePicture
18 position 4). Switch the instrument ON and start the vacuum pump by DynLab (see Picture 21
34 Rev. 3
Output 8 Aspirating pump). When the sensor is correctly set the control LEDs switch at about 60
mBar of the vaccum value. After the vacuum pump is stopped, the vacuum pressure can be reduced
by hand by pressing one of the pinch valves manually. LEDs back switching occurs at about 70
mBar.

Picture 39

In the case of the sensor adjustment change, the inlet tube (1) must be disconnected. A new value
can be set by turning the adjusting screw (2) at the sensor input. Then put the tube back and re-
check the negative pressure for switching. By tightening, the underpressure value at which the
switching occurs increases.

5.5 The camera system adjustment

5.5.1 The camera control software

For service purposes, you can control the camera using the uEye Cockpit software that is installed
along with the Dynablot Automatic application software.

Software is started using the icon , and

selecting the parameters No profile . If the cable is connected from the instrument camera’s
connector to the PC (see Picture 2 position 6), the camera is connected by pressing the button

at the top bar. The live view of the camera is displayed in the window and the camera type
and serial number in the top bar.

35 Rev. 3
The subject of this manual is not a description of all the uEye Cockpit features. Described are only
the functions used to set up Dynablot Automatic.

File of parameters
The camera setting is stored in the parameter file. Besides the file includes AOI, baud rate and
shutter speed. The file is stored in the memory of each camera. For archiving, the file can be saved
in a PC. These archive files are stored by manufacturer for each manufactured intrument under the
name Camera ini xxxx_xxxx_YYMMDD.ini. During service change, new camera setting can be
restored. The menu File / Load parameters a Save parameters is used to manipulate the file of
parameters.

Load parameters / from File ... - the dialog for selecting the .ini file opens. Once the selection is
confirmed, the live preview will begin to appear according to the new
settings.
Load parameters / Parameter set – it retrieves the previously saved setting from the camera’s
memory and the live preview starts displaying according to the new
setting
Save parameters / to File ... – the current setting is saved to the .ini file
Save parameters / Parameter set – the current setting is stored into the camera’s memory

Function of some other buttons

- live viewing closing and disconnecting the camera

- scaling the live view of the PC screen. Scale 1:2 shows the entire shot.

- Opens a window to view and set camera display parameters. The parameter groups are
divided into individual bookmarks.

36 Rev. 3
For service activities are mainly used these parameters:
Camera / Exposure time – setting the shutter speed changes the brightness of the live preview
Size / AOI Width,Height,Left,Top - parameters changing the size and position of the area of interest
from the overall camera view. These parameters are set so that the
shot taken by camera contains just one well with a strip. In the
instrument’s overall setting, these parameters are related to the
setting of the position coordinates of the arm to capture strip images.

- opens / closes the window showing the signal level from the selected line of the camera shot.

Picture 40

A live preview is displayed in the main part of the window (in this case, the AOI is already limited for
the size of the well). When you turn on the signal display function, the line will appear in the preview
(1). Its position is changing by dragging the mouse and is fixed with the left mouse button. You can
change the position again by clicking on the cursor on the line. The window (2) with a brightness
value graph on the row selected by the line is also displayed. The X axis shows the position of the
points in the image, the Y axis is a brightness value in the range of 0 – 255 (0 = no light, 255 =
maximum brightness or overexposed).

37 Rev. 3
 5.5.2 Mechanical position of camera adjustment

The mechanical adjustment of the camera is performed with the removed cover of the working arm
(see Picture 6). A tray is inserted into the holder. Using DynLab software, the holder is set to a
horizontal position and the light illuminates – 33 Camera light output.

Picture 41

Using the uEye Cockpit, the camera will be connected and a full view will be displayed in the live
view. The exposure time is set so that the shape of the wells is clearly visible in the picture.

Picture 42

You can see the LED lighting 1 – trinity next to the mirror (1) and the tray image in the mirror (2).
The position of the camera is not still adjusted in this case.
The position must be adjusted so that the wells are in the center of the shot in the longitudinal
direction and parallel to the edge of the frame.

38 Rev. 3
 1

The position of the camera can be changed when two

fixing screws (1) are partially released. To be accessible,
the pipette module must be moved to the rear position.

Picture 43

2 1

Picture. 44

Adjusting the well image to the center of the shot is done by manually rotating the camera in the
direction (1). Parallelism is adjusted by tightnening the screw that is below or above the camera as
required (2). After setting the camera position, both fixing screws must be tightened.

5.5.3 Area of Interest (AOI) adjustment

The area of interest of the camera is set so that the shot contains one well.

39 Rev. 3
 1

Picture 45

The area of interest (2) is set at about half the height of the shot. Care must be taken not to interfere
with the glare (1) caused by the LED on the lens. The area of interest must be safely distant from
the edge of the mirror (3).

Once the AOI is set, the live preview is limited to

the selected area.

Picture 46

5.5.4 Illumination, camera aperture and focus adjustment

The illumination of the scanned strip is realized by means of two triplets of LEDs (see 8.9 Capturing
strip images). Adjusting the illumination is to set the position of an individual LEDs so that the area
of interest (AOI) captured by the camera is illuminated uniformly. Sharp changes of brightness are
inadmissible in view of the correct evaluation of strip images.
The lighting lights up with the DynLab SW before adjusting, and a sheet of paper is placed on the
plate holder.

40 Rev. 3
 Picture 47

By changing the position of the individual LEDs by bending their leads, the presetting is done so that
their light traces are in the lens axis and the order see Picture 47.

Tracking the light trail of one LED can be

achieved by shading the other five LEDs
using the sleeves.

Picture 48

The next step is to balance the intensity of the light in the AOI of the camera. A calibration plate is
inserted into the plate holder.

41 Rev. 3
 Picture 49

Use the uEye Cockpit to show live view of the camera. By downloading parameters from the camera
(File / Load parameters / Parameter set) , only the AOI is displayed. The exposure time is set to 32
ms.
1

Picture 50

The white strip (1) from the calibration plate (fourth well) is displayed in the preview by changing the
X position of the arm. Next, a window showing the signal level from the selected camera line opens
(2) and the line of the tracked row (3) is set to the center of the well.
Subsequent alignment requires that the brightness curve (4) should be uniform and near the top of
the window (the intensity of the brightest part of the strip will be a maximum of 255).
Uniformity adjustment is first achieved by gentle changes in the position of the LEDs and
subsequently by adjusting the intensity of the individual LEDs. To achieve overall brightness, the
camera lens aperture is used.

42 Rev. 3
 4 5
1

Picture 51

Change of the LED intensity is made by adjusting the potentiometers 1, 2 and 3.

The aperture is adjusted by turning the ring (4) on the camera lens. Caution – ring position is fixed
by screw. It must be released before manipulation. The lens also has a focusing ring (5).

Picture 52

After adjustment, an example of the brightness curve is shown in Picture 52.

NOTE
When adjusting the LED position, the mirror surface may become dirty by
touching the hand. This results in unevenness in brightness. Therefore,
clean the mirrors if necessary.

43 Rev. 3
 5.6 Setting barcode reader
The reader mechanical adjustment must meet two basic conditions to achieve reliable reading of the
barcodes.

80 mm

Picture 53

The viewing angle of the reader must cover the length of the largest sample tube inserted into the
rack in the vertical direction. The lower limit is given by the size of the cutout in the tube rack, the
upper by the tube height.

5°

Picture 54

From the top view, the reader beam must deviate from the perpendicular direction by about 5° to
suppress glare that prevents reliable code reading.

1
2

Picture 55

The entire reader mechanism is adjusted during instrument production.However, the position of the
viewing angle can be gently adjusted by rotating the reader body after releasing the two screws (1).

44 Rev. 3
Deviation from the perpendicular direction can be slightly adjusted by rotating of the entire mirror
assembly after releasing the two screws (2).

Position of the reader check with the DynLab SW

A tube measuring 100 x 16 mm (maximum size for the rack) with a code at a height of 15 mm from
the bottom (corresponding to the lower edge of the cutout in the rack) is used for testing.
After switching on the instrument and DynLab connection, the reader is initialized by sending the D-
code: BCR 9 and BCR 12.
Subsequently, by sending the BCR 2 instruction, the reader is initialized. The red light switches on
the reader. The light switches OFF immediately if the code is successfully scanned or after 5 seconds
of light.

Picture 56

The tube is inserted into some position in the rack. The reading is initialized and the arm is moved
by the hand to the reading position. Once the location is found, the reader is switched OFF almost
immediately after every initialization. Subsequently, the tube is ejected so that the position of the
code matches the maximum upper limit and the reading check is repeated.

45 Rev. 3
 5.7 Coordinate settings using i-parameters
The fine adjustment of the instrument is achieved by setting the coordinates with i-parameters. This
corrects the mechanical differences between the instruments and the settings of the home sensors.
The DynLab SW is used to find the correct coordinate values and store them into the i-parameters
(see 3.3 Setting the instrument parameters and storing in FLASH memory)

5.7.1 Setting i-parameter of position X above the priming cuvette

In the correct position, the aspiration tube of the aspiration arm

is near the right inner wall of the cuvette.

Picture 57

The position is set by changing the parameter R1051 14 Offset 2 X in the Motors Calculation tab as
follows:
 Set the low movement speed X by sending the instruction MMC 1 10 10.
 Move the X arm with the relative feed in the Component Control window by about 10 cm to
the right of the left edge position.
 The X arm is sent by motor to the home position
 The X arm is sent by motor to the absolute position 0.
 Subsequently, using a small relative shift (5 steps), the correct arm position is found (see
PicturePicture 57).
 The value of the Absolute C. column (the last column in the motors control table) is added to
the existing values of i-parameter R1051 and the parameters are written to the FLASH
memory. R1051 typically ranges from -10 to -100.
You can check this setting by repeatedly moving to the home position and sending it to the absolute
position 0.

5.7.2 Setting i-parameter of rocking control

The correct value is set by changing the R1205 51 Check point Rocking in the tab Motors movement
checking as follows:
 Set the low rocking speed by sending the instruction MMC 5 50 50.
 By using the relative movement of the Rocking Motor the home position must be left.
Indicating LED (see Picture 18 position 3) must be switched OFF.
 Rocking is sent to the home position with Rocking Motor.
 Continuous rocking operation is started by sending the absolute coordinate 6000.
46 Rev. 3
  The home sensor LED is monitored and when it is OFF, the absolute position 20 is sent. The
rocking should stop and the LED should light up
 Using a relative shift + with 5 steps value, a position is searched for when the LED switches
OFF.
 The value from the Absolute C. column is written to the i-parameter R1205 and the
parameters are written to the FLASH memory

5.7.3 Setting i-parameter coordinates and constants (Instrument coordinates

and constants)

The manual control is used to set the instrument components to the correct positions when searching
for coordinate values (see 3.2 Manual control of individual instrument components). After setting the
correct position, the coordinate value (Absolute C. column) is written in FLASH Value column in the
appropriate row of the parameter table. During setup the parameters values can be stored at any
time into FLASH instrument memory, but at latest before closing DynLab SW.

Prior to coordinates setup, you need to initialize all movement by searching for the home position
and setting the correct feed rate parameters. It can be accomplished by running the selftest segment
(see 9.1.1 001 selftest RUN.Dcod). Prior to starting, SET R1 0 is set, so only a part of the selftest is
set to set the home positions.

Overview of parameters:
Index Name Typical value
R1566 001 X BCR selftest control code 150
R1551 002 X BCR mirror right side turn 6300
R1547 003 X BCR rear row control code 1000
R1548 004 X BCR rear row 1. tube 1230
R1549 005 X BCR front row control code 5800
R1550 006 X BCR front row 1. tube 5500
R1544 011 Rocking - down position 1200
R1543 012 Rocking - horizontal position 2050
R1542 013 Rocking - up position 2950
R1535 020 Y needle cleaning bowl 8340
R1532 021 Y aspiration ready 13450
R1565 022 Y aspiration down - priming bowl 400
R1528 023 X 1. well - aspiration 1500
R1533 024 Y aspiration down - strip well 300
R1530 025 X pipettor offset -130
R1534 026 Y needle - well pipetting 13400
027 Z well start of level detection for
R1538 dispensing 150
028 Z well stop of level detection for
R1539 dispensing 1000
R1531 035 X camera offset 540
R1540 036 X Camera test label offset 1695
R1545 041 X - needle - 1. tube 1790
R1536 042 Y needle - center front tube row 4175
R1537 043 Y needle - center rear tube row 275
R1574 051 X Control tube A 1526
R1578 052 Y Control tube A 4445

47 Rev. 3
R1575 053 X Control tube B 1526
R1579 054 Y Control tube B -45
R1576 055 X Control tube C 6250
R1580 056 Y Control tube C 4475
R1577 057 X Control tube D 6250
R1581 058 Y Control tube D -25
R1567 061 X pump autocalibration bowl 545
R1568 062 Y pump autocalibration bowl 12710
R1570 063 Z autocalibration bowl top 450
064Z needle position for watching of a-cal.
R1569 filling 1300

001 X BCR selftest control code

X shift position for reading of the control barcode (see Picture 16 position 2) during selftest.

The barcode reader must be initialized after switching on the instrument by sending these instructons
BCR 9
BCR 12
Sending the BCR 2 instruction starts reading. The reader switches ON the red light. The light turns
OFF immediately if the code is successfully scanned or after 5 seconds.
At the correct coordinate, the reader only briefly flashes after initialization. Coordinate is searching
in the direction from the home position. About 20 steps must be added to the value of coordinate at
which the reading starts to be succesful.

002 X BCR mirror right side turn

Position on the right side of the X shift to rotate the mirror of the barcode reader.
Adjustment is done by moving X between the coordinate 0 and the desired coordinate. At the correct
value, the mirror realibly flips. At the same time, the X shift must not hit the right edge of the travel
range.

003 X BCR rear row control code

X shift position to read the control barcode of the back row in the tube rack (see Picture 8 position 2
).
The setup procedure is similar to the parameter 001 X BCR selftest control code.

004 X BCR rear row 1. tube

X shift position for reading the barcode of the first row tube in the tube rack (position number 2).

The setup procedure is similar to the 001 X BCR selftest control code parameter. A 5-step relative
shift is used for stepping. The correct value of the parameter is the coordinate from the left direction
when the reading starts to be successful and this value is incremented by 5.

005 X BCR front row control code

X shift position for reading front line control code in the tube rack.

Prior to setting, the X motor is sent to the 002 X BCR mirror right side turn to turn the mirror to the
reading position of the front row of test tubes. The procedure is similar to 001 X BCR selftest control
code, with the difference that the search direction is from right to left and the value 20 is subtracted.

48 Rev. 3
006 X BCR front row 1. tube
X shift position for reading the barcode of the first tube in the tube rack (position number 43).

The setup procedure is similar to the 001 X BCR selftest control code parameter. A 5-step relative
shift is used for stepping. The correct value of the parameter is the coordinate from the right direction
when the reading starts to be successful and this value is reduced by 5.

011 Rocking - down position

Bottom dead center position of the tray holder.

The coordinate value is found out by stepping with the Rocking Motor from the starting position.

012 Rocking - horizontal position

Horizontal position of the tray holder.

The coordinate values is found out by stepping from the starting position through the bottom dead
center to the horizontal position.

You can use a small water-level for more

accurate horizontal positioning.

Picture 58

013 Rocking - up position

Position of the upper dead center of the plate holder.

The coordinate values is found out by stepping from the starting position through the bottom dead
center to the upper dead center.

020 Y needle cleaning bowl

Position of the pipette module (Y Motor) so that the needle is above the center of the cleaning
cuvette.

49 Rev. 3
For a more accurate positioning, it is advisable
to lower the needle position by moving the Z
shift.
It is important that the needle should be routed
to the hole in the bottom of the cleaning
cuvette (see Picture 36 ).

Picture 59

CAUTION
If the needle is inserted into the cleaning cuvette, change the Y motor
position obliquely to avoid collision of the needle with the cuvette to damage
them.

021 Y aspiration ready

Position of the pipette module (Y motor) so that the control pin just touches the caliper on the
aspiration arm.

Picture 60

022 Y aspiration down - priming bowl

Relative shift of the pipette module (Y Motor) from position 021 Y aspiration ready to get the
aspiration arm down into the priming bowl.

When setting, the Y motor is sent to the absolute coordinate 021 Y aspiration ready. Then, by relative
shift, the value, at which the arm is moved down so that the aspirating tube lightly touches the bottom
of the bowl or just above it, is found.

023 X 1. well - aspiration

X shift position for the tube of the aspiration arm above the center of the first well of the tray.

50 Rev. 3
When setting up, the tray holder is in the upper
position. The position can be better monitored by
manually moving the arm down.

Picture 61

024 Y aspiration down - strip well

Relative shift of the pipette module (Y Motor) from position 021 Y aspiration ready to get the
aspiration arm down into the well of the tray.

When setting, the Y motor is sent to the absolute coordinate 021 Y aspiration ready. Then, by relative
shift, the value, at which the arm is moved down so that the aspirating tube lightly touches the bottom
of the well or just above it, is found.

025 X pipettor offset

Relative shift of X motor from the position 023 X 1. well - aspiration so that the needle is above the
center of the first well of the tray.

When setting up, X motor is sent to the absolute coordinate 023 X 1. well - aspiration. Then, using a
relative shift, the value, at which the needle is above the center of the first well (because it is a shift
toward the home position, the coordinate will have a negative value), is found.

026 Y needle - well pipetting

Position of the pipette module (Y Motor) in which sample pipetting is perfomed into the wells.

In the correct position, the needle is above the flat part of the bottom of the well where the sample is
pipetted. The coordinate value must not be greater than the 021 Y aspiration ready value to avoid
unwanted movement of the aspiration arm.

027 Z well start of level detection for dispensing

When pipetting the sample into the well, the needle searches for the reagent level in the well.
Therefore, pipetting occurs when the needle touches the level of the reagent in the well, leaving the
reminder of the sample not dropping at the end of the needle.

With the correct coordinate position, the needle is about 3 mm above the top edge of the tray.

028 Z well stop of level detection for dispensing

It is related to the previous parameter. If the level is not found, the Z motion is terminated at this
coordinate.

51 Rev. 3
With the correct coordinate position, the needle is about 1 mm above the bottom of the well. The
needle must not touch the bottom of the well.

035 X camera offset

Relative shift of X Motor from the position 023 X 1. well - aspiration so that the first well of the plate
is in the camera’s shot.

Before setting this parameter, the camera settings must be done according to chapter 5.5 Setting
the camera system.
Adjusting is done using live camera preview (SW uEye Cockpit). The arm moves to the absolute
coordinate 023 X 1. well - aspiration. The holder with the inserted tray is in the horizontal position.
Then relative coordinate, at which the first well is in the center of the camera shot, is found.

036 X Camera test label offset

X shift position to display the Camera test label at the center of the camera shot.

This is the absolute coordinate of the X motor when the label fills the entire camera shot.

041 X - needle - 1. tube and 042 Y needle - center front tube row
X and Y shift positions for the needle above the center of theposition 1 in the tube rack.

For setting up, it is preferable to use auxiliary cylinders that have the dimensions as the maximum
tube and the center indicated. The needle is positioned in the center of the cylinder with X, Y and Z
shifts. Absolute values of X and Y coordinates of the motors are written into parameters.

Picture 62

043 Y needle - center rear tube row

Y shift position for the needle above the center of position 2, the rear row in the tube rack.

Setting similar to previous parameters. Coordinate 041 X - needle - 1. tube is common for the front
and rear row of tube positions.
52 Rev. 3
051 X Contol tube A and 052 X Contol tube A
X and Y shift positons for the needle above the center of the position for the control tube A in the
tube rack.

For setting up, it is preferable to use tubes with a lid and centerline. The needle moves X, Y and Z
feed onto the lids. Absolute values of X and Y coordinates of the motors are written into parameters.

Picture 63

053 X Contol tube B and 054 X Contol tube B

055 X Contol tube C and 056 X Contol tube C

057 X Contol tube D and 058 X Contol tube D

X and Y shift positions for the control tubes B, C and D similar as to position A.

061 X pump autocalibration bowl and 062 Y pump autocalibration bowl

X and Y shift positions for the needle above the center of the square hole in the cuvette for the
peristaltic pumps calibration.

The needle is positioned in the center of the hole by X,

Y and Z shifts. Absolute values of X and Y coordinates
of the motors are written into parameters.

Picture 64

53 Rev. 3
063 Z autocalibration bowl top
Z shift position for the needle at the top edge of the square hole.

Setting the correct position is important for proper

calculation when the pumps are calibrated.

Picture 65

064 Z needle position for watching of a-cal. filling

The position Z, in which the needle waits for the touch of the liquid during the filling of the calibration
cuvette by the pump.
The correct position is about 3 mm above the square hole lower orifice into the main cavity of the
calibration cuvettes.

When calibrating the pump, the last batch must partially fill
space of the aperture hole, where the water level is detected by
by the needle.
If the needle was too low, the pumped water would only
remain in the main cavity of the cuvette.
If the needle was too high, the cuvette would be overflowed.

Picture 66

54 Rev. 3
6 Instrument maintenance
The individual chapters describe the assembly procedures used for routine maintenance. Some of
these procedures are used to perform a check and preventative maintenance of the intrument.

6.1 The instrument checking and preventive maintenance

operations list
Checking and preventive maintenance of the instrument should be carried out at annual intervals, in
the normal operation of the instrument.

Step Operation Note / Link

1 The cassettes of peristaltic pumps 6.3.1 Pump cassette - replacement
replacement
2 The reagent tubing replacement 6.3.2 Filling tubes - replacement
6.3.3 Tubes for reagent bottles - replacement
3 Pumps calibration see Service manual, kap. 4.4 Pump calibration

4 X shift control and maintenance 6.7 Shift maintenance / 6.7.1 X - shift

5 Y shift control and maintenance 6.7 Shift maintenance / 6.7.2 Y - shift

6 X shift control and maintenance 6.7 Shift maintenance / 6.7.3 Z - shift

7 Rocking motion control and 6.7 Shift maintenance / 6.7.4 Rocking

maintenance
8 Checking and maintenance of the Check vacuum and adjust if necessary
vacuum in the waste bottle system - 5.4 Vacuum in the waste bottle adjustment
Cleaing the reverse throttle valve - 6.8 Maintenance of
exhaust silencer and reverse throttle valve
9 Checking of system solution pump 6.6.1 Status test and replacement of the system solution
pump
10 Maintenance of barcode reader 6.9 Maintenance of barcode reader
system
11 Camera system maintenance 6.10 Camera system maintenance

12 Calibration of camera system Calibration (see SW for strip evaluation - LabImage)

13 Checking of the instrument Running the protocol Instrument Check (see 6.15
functionality checking Checking of the instrument functionality)

List of spare parts

Name Catalog no. Pcs.
Tubing replacement maintenance kit - Reagent part DA21-09010 1

55 Rev. 3
 6.2 Disassembly the instrument covers
6.2.1 Front cover - disassembly

CAUTION
The instrument must be turned OFF when the cover is removed and
replaced. Otherwise, damage of the electronic parts may be caused by
manipulation with the stepper motor connector.

The cover is released by unscrewing the four screws (see Picture 67 ).

Picture 67

After lifting the front of the lid, disconnect the faston ground wire and, after partially removing the left
side of the cover, disconnect the power connectors of the rocking stepping motor with its home
sensor and the heating foil with temperature sensor. When disconnecting the connectors, the ratched
on the top of the connectors must be pressed (see Picture 68).

Picture 68

Subsequently, the cover can be removed from the instrument.

6.2.2 Rear cover - disassembly

Before disassembling the rear cover, remove the cap and disconnect the ground wire faston (see
Picture 69).

56 Rev. 3
 Picture 69

Because the tube rack is inserted into the cover, the position of the cover affects the setting of some
movements coordinate in i-parameters. In order to mount the cover to the same position later, it is
fitted at the opposite ends with an adhesive tape with a dashed line for later alignment. The tape is
cut at the place of the covers gap. Release the cover by unscrewing the five screws (see Picture 70
).

Picture 70

Subsequently, the cover can be removed from the instrument.

6.2.3 Workspace cover - disassembly

Picture 71

57 Rev. 3
On the hinges of the workspace area cover, the bolts are released and the fuses are opened. On
the left side, the faston ground conductor must be disconnected.

Picture 72

On the rear wall of the device, the bolts are loosened and the spring struts are flipped. The struts
are removed from the brackets. CAUTION - After removing the struts, the cover must be held in
place to prevent it from falling to the closed position.

Picture 73

Subsequently, the cover can be lifted and positioned next to the instrument.

58 Rev. 3
 6.3 Replacement of pump cassettes and reagent tubes
6.3.1 Pump cassette - replacement

Remove the two pipe connectors from the old

pump cassette. The ratchets are pressed at the
sides and the cassette is pulled down from the
engine axes.

Picture 74

Before installing the new cassette, it is advisable to

roughen the motor axis longitudinally with fine
sandpaper and clean with alcohol.

Picture 75

59 Rev. 3
The new cartridge is put on the motor axis and
pressed to snap the ratchets.

Picture 76

6.3.2 Filling tubes - replacement

The front cover of the instrument must be removed to replace the filling tubes (see 6.2.1 Front cover
- disassembly) and a set Dispensing tube set DA21 – 08116 is needed.

Picture 77

1 - 8 pieces of tubes marked with sleeves. The first number is the number of the tube position on the
drawing of Appendix 9.4 Hydraulic scheme. The number in brackets (1 - 8) is the name of the channel
to which the tube belongs.
2 - 3 pieces of cable ties
3 - 8 pieces of connector for connecting the tubes to the cassette of the peristaltic pump

60 Rev. 3
 1

2
4

Picture 78

The bolt (1) is unscrewed and the plastic tube holder (2) is pulled out of the filling arm. Remove the
tube cover (3) and cut off the cable ties (4). Then the old tubes are disconnected from the pump
cassette connectors and removed from the unit. The plastic holder is removed from the tubing for
further use.

8 7 6 5 4 3 2 1

Picture 79

The new tubes from the kit are inserted into the plastic holder in the order of channels (number in
bracket on the sleeve) according to Picture 79 .

The plastic tube holder is inserted into the filling

arm and secured with a screw. The ends of the
tubes are aligned to protrude about 5 mm below
the arm.

Picture 80

61 Rev. 3
 1

Picture 81

The tubes are successively inserted in the notches (1) behind the stop (2) from the 8th to the first
channel and pushed into the grooves (3) in the arm.

The result is a regular distribution of the

tubes so that they do not interfere with the
lower edge of the lighting mirror and do not
cause the shadow on the strips during their
scanning.

Picture 82

Using cable ties, the trinity of the tubes (in the

right three, one tube belonging to the system
solution pump) is attached to the lower part
of the arm. WARNING - Do not tighten the
ties too much to avoid flow reduction due to
tightening of the tubing.

Picture 83

62 Rev. 3
 Picture 84

The tubes are pushed through the holes in the front of the instrument to the respective pumps, and
connects through the connectors to the right tubes of the cassettes and the tubes are drawn into the
device. Care should be taken to regularly arrange the tubes under the front cover, without
unnecessary crossing, so that they do not lift too much when the arm is moving and do not engage
with the rocking mechanism.

6.3.3 Tubes for reagent bottles - replacement

The Bottle tubing set DA21 – 08121 is required to replace the filling tubes.

Picture 85

1 - 8 pieces of tubes marked with sleeves. The number on the sleeve indicates the channel that the
tube belongs to
2 - 8 pieces of connector for connecting the tubes to the cassettes of the peristaltic pump

63 Rev. 3
A new tube with a connector connects to the left
tube of the appropriate peristaltic pump.

Picture 86

6.4 Exchange of aspiration tubes

The DA21 – 08122 set is required to replace the tubes aspirating the waste from the wells of the
tray and from the needle cleaning cuvette.

Picture 87

1 - 4 pieces of tubes marked with sleeves. The number indicates the position of the tube in the
drawing of Appendix 9.4 Hydraulic
2 – cable tie
3 - Y-connector for connecting the tubes in front the waste bottle

Before replacing the tubes, remove the rear cover (see 6.2.2 Rear cover - disassembly). For a more
comfortable access from the rear to the bottom of the working arm, it is recommended to remove the
workspace cover (see 6.2.3 Workspace cover - disassembly).

64 Rev. 3
 2

Picture 88

At the bottom of the working arm, cut the tie (1) to release the old tubes. Remove the cover (2) from
the rear of the arm by unscrewing four screws.
After removing the old tubes, proceed to install new ones.

38

Picture 89

The tube marked by a sleeve with the number 38 is pushed through the opening (1) in the working
arm from behind and is attached to the aspiration arm (2).

1
38

37

65 Rev. 3
 Picture 90

The tube marked by a sleeve with the number 37 is attached to the needle cleaning cuvette (1). The
two tubes are fastened with the cable tie (2). CAUTION - The tie must not be tightened too much to
avoid flow reduction due to tightening of the tubing.

37

38

37

Picture 91

Next, the two tubes are inserted into the holders on the movable chain and on the mainboard cover
(see Picture 91). The tube 37 (from the aspiration arm) is at the bottom cutout and the tube 38 (from
the needle cleaning cuvette) is in the upper cut out of the chain. On the mainboard, this is the
opposite (see Picture 91).

39

38

37

Picture 92

The tubes are inserted through the grommets in the back of the instrument. It is important that the
respective tubes come to the correct pinch valve. The tubes are coupled using a Y-connector and a
tube sleeve number 39 with a W output of the waste bottle (see Picture 92).

66 Rev. 3
 1

Picture 93

The tubes are inserted into the notch in the pinch valves. CAUTION - Between the bushing and the
valve, the tubes must form a regular arc (1) to avoid flow reduction due to tightening of the tubes.
For the correct operation of the valves, the tubes must be carefully up and down inserted into the
rear of the notch (2). (see Picture 93).

The tube with sleeve No. 36 belongs to the

aspiration of the pumps calibration cuvette.

36

Picture 94

6.5 Replacing the vacuum system tubing

To replace the vacuum system tubing, the front cover (see 6.2.1 Front cover - disassembly), rear
(see 6.2.2 Rear cover - disassembly) and the right cover above the motors of the peristaltic pumps
must be removed. You need a set Vacuum tube set DA21 - 08123.

67 Rev. 3
 1

Picture 95

The drawing in the Appendix 9.4 Hydraulic is used to determine the purpose of the set items. Some
tubes are marked with a sleeve with the position number in the drawing (29, 30, 31, 33 and 35).
Unlabeled items in drawing are:
1 - position 34
2 - position 53 - inserted silencer
3 - position 32

The old tubes are dismantled. The vacuum system is again assembled from the new set according
to Picture 96. The individual parts are shown in the picture with the position shown on the Hydraulic
scheme. The position tube 35 is connected to the output V in the waste bottle.
34

32 35

31

30 32

33

51

68 Rev. 3
 35

29

30

Picture 96

Picture 97

It is necessary to orientate according to Picture 97 when mounting the reverse throttle valve.

6.6 Replacing the pipette system components

6.6.1 Status test and replacement of the system solution pump

In order to clean the needle between pipetting of individual samples, the peristaltic pump of the
system solution must be in a satisfactory condition. Pump status can be verified by a simple test
using the pipetting system purge function:
The instrument turns on and connects to the DynLab SW.
First, the initialization is required for all moves by searching for the home positions and setting the
correct shift parameters. This can be accomplished by running the selftest segment (see 9.1.1 001
selftest RUN.Dcod ).
Prior to starting, SET R1 0 is set, so only a part of the selftest is set to set the starting positions.

69 Rev. 3
The measuring cylinder is filled with 40 ml of distilled water
and the cap with a suction tube is put to it (see Picture 98).
The segment 020 System voiding RUN (see 9.1.2 020 System
voiding RUN) is ran.
The needle goes into the cleaning cuvette and the system
solution pump draws up. After the segment runs, the water
loss in the cylinder is checked.
Typical water consumption for one run of the segment is 19-
20 ml. If the consumption is less than 17 ml (e.g. more than 23
ml remained in the cylinder), replace the pump cassette (see
6.3.1 Pump cassette - replacement).

Picture 98

NOTE
The system solution pump can also be run from the Dynblot Automatic
application SW using the Instrument maintenance / system solution /
Empty.

6.6.2 Replacing the system solution tubes

To replace the system solution tubes, the front cover of the instrument must be removed (see 6.2.1
Front cover - disassembly), the rear cover (see 6.2.2 Rear cover - disassembly) and the working arm
cover. For a more comfortable access from the rear to the bottom of the working arm, it is also
recommended to dismantle the workspace cover (see 6.2.3 Workspace cover - disassembly).
The set Dispensing tube set - system liquid DA21 – 08124 is required.

26

10

Picture 99

70 Rev. 3
 Picture 100

To obtain access to the tubes, remove the caps from the front and rear parts of the working arm
(Picture 100).
Old tubes are replaced according to the pictures. The individual parts are indicated in the pictures
by the position shown in Appendix 9.4 Hydraulic .

22

21

Connection between the pump and the bottle

cap of the system solution (21) including the
pump tube connector.

Picture 101

25

22

24

Picture 102

71 Rev. 3
When connecting the pump and the three-way valve, the tube (22) passes through the opening (1)
in the working arm. The front is connected by a connector to the right tube of the peristaltic pump. In
the working arm area, it is connected to the three-way valve input 1 (COM).

25

26

Picture 103

The tubes (25) and (26) are used for the connection between the output 2 (NC) of three-way valve
and the syringe. The assembly of the tubing to the syringe is shown in Picture 103. When handling
the tube, the connector on the syringe head must be loosen. The new tube is inserted into the
connector and then it is tightened.

24

The connection between the output 3 (NO) of the

three-way valve and the needle cleaning cuvette
(1) is done by a tube (24) guided along the back of
the working arm.

Picture 104

6.6.3 Replacement of needle and tubes

To replace the needle and its tubes, the cover of the working arm and the pipette cover must be
removed. The tubes are in the Dispensing tube set - pipetting DA21 - 08125.

72 Rev. 3
 27

28

Picture 105

9.4 Hydraulic is used to determine the purpose of the set items. The silicon tube (1) is designed to
be threaded onto a tube (27) for fastening to the holder (see next text).
28 27 1 2 3 4 27

Picture 106

To release the pipette tube, loosen and unclip the holder (1) on the pipette module, remove the
protective spiral (3), and cut off the cable tie (4) in the working arm area. Remove the tube from the
needle with a silicone junction. From the head of the syringe, the tube can be pulled out after the
connector is released.

28

27

73 Rev. 3
 Picture 107

When fitting a new tube, when attaching the junction (28) to the needle, careful caution is required
so that the edge of the needle does not cut off the junction wall. Even a very small hole would cause
inaccuracies in pipetting. When the connector on the syringe head is loosen, the tube (27) is inserted
in it and the connector is tightened.

The newly mounted tube is fastened with a strap to the holder, together with the level detection cable
with a protective spiral and fixed by the holder on the pipette module. For correct fixation, a piece of
silicone tubing must be fitted at the teflon tube in the
place of the holder (27) (see Picture 106 position 1).

When removing the needle, the inlet tube is

disconnected. The needle lock (1) is loose and tilted.
The needle can be pulled out of the tube in the needle
holder and replaced with a new one.

Picture 108

6.7 Shift maintenance

6.7.1 X - shift

For X shift, care must be taken

to ensure that the rails (1) are
clean. During maintenance,
the rails are cleaned from the
top and bottom by alcohol and
gently lubricated with oil.

Picture 109

74 Rev. 3
 1

If the belt is to be tensioned, four bolts (1)

are released, tensioning the belt on the
pulley and tightening the bolts again.

Picture 110

6.7.2 Y - shift

For Y shift , care must be taken to ensure that the rails (1) are clean. During maintenance, the rails
are cleaned from the top and bottom by alcohol and gently lubricated with oil.
The toothed ridge (2) must be sufficiently greased with vaseline.

75 Rev. 3
 6.7.3 Z – shift

For Z shift, care must be taken to ensure that the rails

(1) are clean. During maintenance, the rails from
both sides are cleaned with alcohol and gently
lubricated with oil.
Also the drive screw (2) is gently lubricated with oil.

Picture 111

6.7.4 Rocking

For proper operation of the mechanism, the pulley (1)

must rotate freely and contain a rubber o-ring to prevent it
from sliding on the eccentric wheel (2). In case of damage
to a part, it is necessary to replace it.

Picture 112

76 Rev. 3
 1

If the belt tension is required, four bolts (1) are

released, pressure on the pulley is tensioned
and the bolts are secured again.

Obr. 113

6.8 Maintenance of exhaust silencer and reverse throttle

valve
The exhaust silencer (see Picture 16 position 9) and reverse throttle valve (see Picture 13 position
7) may be contamined with residual fluids. The reverse throttle valve may even be glued, resulting
in insufficient vacuum creation in the waste bottle.

The container of the silencer is removed from

the holder and it can be cleaned after the
unscrewing the lid.

Picture 114
1

Picture 115

After turning the valve body halves, the insert (1) is removed, which is thoroughly washed with water
and checked if its spring loaded piston moves freely.
77 Rev. 3
When reassembling, care must be taken to ensure
that the insert is correctly positioned so that the
valve is permeable in the direction indicated by the
arrows.
The direction of arrows from the waste bottle to the
vacuum pump must also be observed when
connecting the valve to the tubes.

Picture 116

6.9 Maintenance of barcode reader

Picture 117

To ensure that the barcode reader functions correctly, make sure that the visor cap (1) and the mirror
(2) are clean. A fine cloth intended for maintenance of the optics is used for cleaning. Alcohol may
be used on the mirror when it is contaminated. Caution - the visor cap is made of plastic, and if
chemicals are used for cleaning, it could be damaged.

78 Rev. 3
 6.10 Camera system maintenance

Picture 118

For proper operation of the camera system, the lens filter (1), the camera mirror (2), and the mirror
for LED lighting (3) must be clean.
A fine cloth intended for maintenance of the optics is used for cleaning. Alcohol may be used on
mirrors when the dirt is heavier.

CAUTION
An optical mirror with a reflective layer applied to the surface is used for
camera to reduce dupplication of the image. Therefore, it is not protected
by glass. It is very important to use a very soft material for cleaning. The
paper towel can cause scratches on the mirror surface.

NOTE
The lens filter and the camera mirror are well protected against dirt by their
position under the cover of the working arm, in a normal operation. If there
is no suspicion of their contamination, it is sufficient to clean the LED mirror
for normal maintenance and the working arm cover must not be removed.

79 Rev. 3
 6.11 Dismantling the syringe

1 3

Picture 119

The cover of the working arm is removed first. To access the four syringe screws (3), the mirror
holder lighting and the LED lighting 2 are diverted after the two screws are released.
The holder remains hanging on the power lines. After the screws (3) are released and the two
connectors are disconnected, the syringe can be removed from the arm.

CAUTION
When manipulating with the holder, the shape of LEDs must not be changed
so the strip lighting settings are not changed.

80 Rev. 3
 3

Picture 120

In case of a damage, only the syringe head (2) can be replaced. Two bolts (1) are released and the
head retracts from the piston (3).

6.12 Dismantling of needle cleaning cuvette

Picture 121

After removing the two screws (1), remove the cuvette in the direction of the arrow from its position
and disconnect the waste tube (2) and tube of the system solution (3).

81 Rev. 3
 5

1 2

Picture 122

To clean the inner part of the cuvette, remove the cap (2) after loosening the four screws (1). For
proper functioning of the cuvette, the inlet port of the system solution (3), the waste hole (4) and the
needle rinse nozzles (5) must be well through and clean.

6.13 Dismantling the peristaltic pump motor

1 2

Picture 123

Remove the peristaltic pump cassette and loosen the two screws (1). The pump motor is ejected
and the power wires are released from the terminal block (2).
WARNING for the correct polarity of the supply wires when reassembling.

82 Rev. 3
 6.14 Dismantling pump of vacuum in waste bottle

Picture 124

Remove the tubes (1) and the connector (2) from the motor assembly. The four screws (3) are
released from the back.

Picture 125

Picture 126

The body of the head can be washed with water so that the contact surfaces of the two valves (1)
are clean.

83 Rev. 3
 6.15 Checking of the instrument functionality
The instrument functionality is verified by running the Instrument Check protocol. This test is
assigned to an assay of the same name (see 9.2 Assay listing of Instrument check).

At least 4 samples are selected when creating a worklist. The barcode reader function is verified by
reading the code from the inserted tubes with a barcode. The type of tubes must be the same as the
type selected in the worklist table, and the tubes are filled with the system solution in the same
volume as the usual volume of samples in routine operation.
Reagent preparation will require distilled water at pump number 8.
In the assay steps, the correct execution of each activity is checked. The confirmation is required,
as with manual activity, before performing any other activity.
In the first step of the assay, 100 μl of the system solution is pipetted from the tubes to the empty
wells. Check for pipetting in each well (100 μl) does the pipettor function (orientative checking for
pipetting can be done by aspiration of 100 μl using a pipette).
In the second step, 2 ml of distilled water is filled into the wells and incubation is then started - rocking
for 30 seconds.
In the third step, the contents of the wells is aspirated.
In the fourth step, one strip drying cycle is run.
In the fifth and the final step, the plate can be replaced by a calibration plate and scanned for strip
images.
Then the protocol run is completed.
Images of the calibration plate can be checked in the detail of the completed protocol in the menu
History / Protocols.

7 History of instruments running- log files

The Dynablot Automatic PC application creates and stores files with the history of the instrument
running. Communication.log files contain a detailed record of the instrument operation, and their
analysis can help to correct the malfunctions. Files with error status records can be sent to service
centers supporting Dynablot Automatic instrument to help resolve technical issues.

The files are stored in the C:\Dynex\DynablotAutomatic\Logs.

The file that is currently being recorded is called communication.log.
Files from previous days are added to the date they were recorded, for example,
communication.log.2017-02-16. If a file size exceeds 1 MB, a new file is created for that day. The
original file is renamed by adding the name to the name with the sequence number .1. If the current
file exceeds the limit size again, a new file is created and all older files of that day are renumbered
as one.
This creates a chronological series of log files ranging from the latest records to older ones. For
example:
communication.log
communication.log.1
communication.log.2017-02-20
communication.log.2017-02-16
communication.log.2017-02-16.1

84 Rev. 3
8 Principles of operation
8.1 Communication
External communication between the PC and the instrument:
This communication is realized via two USB ports.
One communication channel is connected to the mainboard of the intrument and passes data of
commands and information related to the control of the intrument operation.
The second channel directly connects PC and camera and provides camera control and image data
transfer. This USB connection also provides power to the camera, which is therefore independent of
the power supply of the instrument.

Internal communication:
Communication between individual functional units within the instrument
 Mainboard – armboard, is realized via a cable stored in the energy chain between the
instrument chassis and the movable working arm, has its own communication protocol
 Barcode reader – mainboard, is realized via a flat cable between the reader and the armboard
and also by the cable stored in the power chain between the chassis of the instrument and
the movable working arm, the serial communication at the TTL level
 Level detection circuit – mainboard, is realized via a cable stored in the energy chain between
the chassis of the device and the movable working arm, the I2C communication protocol

8.2 Hydraulic scheme of reagent filling system

The instrument has 8 channels to fill the reagents. Each channel is composed of a peristaltic pump.
The inlet tube ending with a plastic tube for insertion into a reagent vial and an outlet tube leading to
the filling arm are connected to it. Simple design of the channel enables easy replacement of parts
during preventive maintenance.

Tubes for reagent Pumps 1-8 Tubes in the instrument

bottles

Filling arm

Picture 127

85 Rev. 3
 8.3 Hydraulic scheme of exhaust system
The aspirating system is based on vacuum in the waste bottle. It ensures that the used system
solution is aspirated out of the needle cleaning cuvette and aspirates the contents of the wells while
replacing the reagents during the protocol run.

Pinch valves for aspiration of W V

Needle cleaning cuvette needle cleanig cuvette

Waste
wells

Waste
bottle

Exhaust
Vacuum Reverse
Pump throttle valve Vacuum

Exhaust Rear side of the instrument

silencer
Vacuum switch

Vacuum regulator

Picture 128

A diaphragm pump is used to create a vacuum in the waste bottle, the outlet of which is fed through
the muffler into the exhaust in the rear wall of the instrument. The silencer reduces the noise of the
instrument and separates any residual liquid that is sucked from the waste bottle. By the vacuum
regulator, the vacuum is set in the waste bottle when both aspiration valves are closed. Depending
on the pressure value on the regulator inlet valve, air is pumped into the pump. Pressure is adjusted
by turning the screw on the top of the regulator. By tightening of the screw, the air suction is limited,
which increases the bottle under pressure. The reverse throttle valve maintains the vacuum in the
waste bottle after the pump has been switched off. This speeds up the system's readiness to run the
next aspiration cycle. By means of a vacuum sensor, the instrument control system receives a signal
whether sufficient aspiration pressure is achieved. By opening the exhaust valves of aspiration for
cleaning cuvette or wells, the aspiration from the appropriate location is activated.

8.4 Hydraulic scheme of pipetting system

The pipetting system performs the sample transfer between the sample tubes in the rack and the
wells in the tray. It is filled by the system solution during its operation. The solution makes the
pipetting more accurate (if the air fills the system its compressibility is problematic) and it is used for
the needle cleaning.

86 Rev. 3
 Syringe Three-way valve System solution pump

Needle

M
System solution
Needle cleaning cuvette bottle

Picture 129

The needle serves to manipulate the sample itself. The surface of the inner walls and the lower part
of the outer wall of the needle are provided with ceramic "nanocoating" technology, which greatly
facilitates its cleaning. The cleaning is done by the peristaltic pump with the flow of the system
solution. Depending on the location of the three-way valve, the solution is passed through the syringe
into the interior of the needle or into the top of the cleaning cuvette. During cleaning, the needle is
partially inserted into the cuvette and the outer part that comes into contact with the sample is
washed.

Accurate measurement of the volume of the pipetted sample is performed in the syringe by piston
movements. The piston is sealed at the bottom of the head of the syringe, the inside diameter of
which is larger than the diameter of the piston. This allows free flow of system solution at any piston
position. When pipetting, the three-way valve outlet leading into the syringe is closed and the syringe
with the needle forms a closed space. The movement of the piston is then accurately transferred to
the system solution in the needle and thus to the pipetted sample. Before taking a sample, a small
amount of air is drawn into the needle tip and this creates a bubble, which separates the sample
from the system solution.

8.5 Fluid level detection with pipettor needle

Liquid level detection using a pipetting needle is used to detect:
 the sample levels in primary tubes before aspirating
 the reagent level in the wells of strip plate before pipetting samples (pipetting below the
level of the well content eliminates the drop of sample on the tip of the needle)
 the presence of a system solution in a needle cleaning cuvette during the system solution
priming
 the presence of a system solution in a needle cleaning cuvette while filling the cuvette during
extra needle cleaning
 the water level in the calibration cuvette during the calibration of peristaltic pumps

For detecting the touch of a pipette needle with a liquid, the principle of the electrical capacity
measuring between the needle and the chassis of the apparatus is used, where the needle forms
one electrode and the chassis of the instrument the second electrode of the measured capacitor.
The capacity measuring circuit is located on the armboard and is connected to the needle by a
shielded cable.

87 Rev. 3
 Capacity
measurement IO
Needle
Measured capacity
The instrument chassis

Picture 130

When looking for a liquid level, the parasitic capacity of the needle against the chassis is first
recorded. Then, the required process is triggered (for example, moving the needle down when
searching for the sample level or starting the system solution pump when filling the cleaning cuvette).
As soon as the needle touches the fluid which is looked for, the fluid capacity is added to the parasitic
needle capacity and the measuring circuit records an increase of the value. If the increase exceeds
the required limit, the level detection is considered to be successful.

Picture 131

INSTRUCTION
The capacity of the liquid to be searched against the chassis of the instrument
is dependent in the electrical conductivity of the liquid. For this reason, it is
important to add a preparation named Setup Clean to the distilled water in the
prescribed concentration when preparing the system solution. Low
concentration decreases the conductivity of the system solution and causes
errors during the preparation of the system solution and during the filling of the
cleaning cuvette during extra needle cleaning.

8.6 Automatic calibration of peristaltic pumps

In the automatic calibration process, the peristaltic pump performs short, time-defined batches that
fill the calibration cuvette. Calibration constant is calculated as the ratio of pumping time to filled
volume.

Picture 132

88 Rev. 3
In the first calibration phase, the needle is at the lower edge of the measuring hole and the calibrated
pump performs filling doses. Once the needle detects fluid contact, the dosage is interrupted. The
fill time is calculated from the number of batches. The level is stabilized in the measuring hole after
the last dose.
Subsequently, the needle goes to the upper position and returns to the measuring hole to measure
the height of the level. The volume is calculated from the known volume of the cuvette main cavity
and the level of the level in the measuring hole.
Then, the liquid from the calibration cuvette is aspirated into the waste bottle and the cuvette is
prepared to calibrate the next pump.

8.7 Level detection in system solution bottle

The level detection in the system solution bottle is carried out in the holder by contactless
measurement using light beams. The presence of the solution is detected in two levels - Warning
and Alarm. A different beam refraction passing through an empty or filled bottle is used for detection.
.

LED Sensors

Warning

Alarm

The level detection is activated only when the system solution is in the Ready state and the
measurement is done once every 10 seconds with a brief LED illumination.

INSTRUCTION
Do not place labels or self-adhesive labels on the system solution bottle at the
measurement level to prevent beam passage.

89 Rev. 3
 8.8 Barcode reader
The bar code reader scans the sample identification codes from the primary tubes inserted into the
rack that is inserted into the instrument. In order to save space, reading is performed via the swivel
mirror.

Rear row of tubes

Reader Rotary mirror

Front row of tubes

Picture 133

The swivel mirror allows you to read the codes from the opposite rows of tubes in the rack. The tilting
of the mirror is performed mechanically when the working arm reaches the utmost right and left X
shifts.

INSTRUCTION
The types of codes, that are recognized during reading, can be selected in the
instrument settings. In terms of reliability of recognition and read speeds, it is
advisable to select only the types that are actually used on the tubes.

90 Rev. 3
 8.9 Capturing strip images
The strip image capture is performed with a 5 MPixel monochrome CCD camera. To achieve the
required distance between the camera lens and the scanned area, the shot is performed over the
sloping mirror. This made it possible to reduce the height of the instrument.

Virtual camera position

Camera

Mirror

Shot area in plate well

Green LEDs are used to illuminate the area being scanned. In order to suppress the influence of
ambient light penetrating into the workspace, the camera lens is equipped with a green filter that
only passes light of the wavelength corresponding to the LED light. Lighting LEDs with a radiation
angle of about 20 ° are placed in two triplets to achieve bottom illumination of the wells without
undesirable shadows from the walls of the wells. At the same time, the LEDs are positioned so that
their direct reflections on the wet surface of the strips are out of the camera view.
By setting the camera parameters, the AOI is selected, which is part of the camera overall shot. Only
this part of the image is then transmitted by the camera when creating a snapshot of a well with a
strip. An appropriate combination of arm and camera position, choice of area of interest, and lighting
adjustment will give a picture of the bottom of the well with a stripe without undesirable shadows and
glare.

Side view View from the top

Area of interest

Virtual
LED position

Border of the
camera shot

Mirror

Picture 134

91 Rev. 3
9 Appendix
9.1 D-code segments
The chapter contains D-code segments that are run from DynLab software for some service
activities.

9.1.1 001 selftest RUN.Dcod

;****Self tests and home positions after the instrument switch ON****
SET R1 0 ; 0=only home positions, 1=all tests
SET R2 1 ;BarCodeReader 0=No 1=Yes
SET R3 0 ;Camera 0=No 1=Yes
SET R400 0
SET R421 1
RUN 0
END

9.1.2 020 System voiding RUN

;***************System voiding (syringe, needle, needle cleaning bowl)**************

SET R400 0
SET R421 20
RUN 0
END

92 Rev. 3
 9.2 Assay listing of Instrument check

Group Instrument check

Assay Instrument check
Step Operation Parameter Value
Pipettting Pipetting samples Volume 100,00 µl
Disable multiple No
pipetting
Extra needle No
cleaning
Clot detection No
Dispensing Manual operation Message Dispensing start
Incubation
Dispensing Filling Volume 20,00 * 0.1 ml
Incubation
Reagents Dist. Water
Dispensing Manual operation Message Incubation start
Incubation
Dispensing Incubation Speed rocking 2
Incubation
Incubation time 00:00:30
After stopping the Yes
incubation, stop the
rocking
Aspiration Manual operation Message Aspiration start
Aspiration Aspiration Thorough No
Aspiration
Drying Manual operation Message Drying start
Drying Drying Drying cycles 1
Images Manual operation Message Insert the
calibration plate -
Images taking start
Images Capturing

93 Rev. 3
 9.3 Preventive maintenance protokol
-----------------------------------------------------------------------------------------------------------------------------------

Preventive maintenance protokol

Instrument : Dynablot Automatic

Serial number : ___________ - ____________

Checklist of operations

Step Operation done

1 The cassettes of peristaltic pumps replacement

2 The reagent tubing replacement

3 Pumps calibration

4 X shift control and maintenance

5 Y shift control and maintenance

6 X shift control and maintenance

7 Rocking motion control and maintenance

8 Checking and maintenance of the vacuum in the waste bottle system

9 Checking of system solution pump

10 Maintenance of barcode reader system

11 Camera system maintenance

12 Calibration of camera system

13 Checking of the instrument functionality checking

Date

Name
Service technician
Signature

94 Rev. 3
9.4 Hydraulic diagram

95 Rev. 3
9.5 Mainboard connector map

JP5 JP4
JP
7

JP JP9 JP8 JP
12 10

JP
11 MAINBOARD

JP19 JP20 JP22 JP23 JP24 JP25 JP26 JP27 JP28 JP18

JP4 - auxiliary service connector for LCD display right fan of workspace area
JP5 - pumps LED indicators boards peristaltic pump of system solution
JP 7 - encoder of X shift power supply of the system solution holder
JP8 - sensor of the closed position of the workspace cover JP24 - level detection in syst. solution bottle and waste bottle
JP9 - vacuum in the waste bottle sensor JP25 - peristaltic pumps channel 1 and 2
JP10 - main cable for connection to the armboard JP26 - peristaltic pumps channel 3 and 4
JP11 - rocking home sensor JP27 - peristaltic pumps channel 5 and 6
JP12 - home sensor of X shift JP28 - peristaltic pumps channel 7 and 8
JP18 - power supply 24 V
JP19 - stepper motor of X shift
JP20 - stepper motor of rocking
JP22 - outputs : power supply of the heating foil regulator
pinch valve of asp. cleaning needle cuvette
pinch valve of asp. arm of the wells
vacuum pump
JP23 - outputs : left fan of workspace area

96 Rev. 3
9.6 Armboard connector map

JP7 JP8
JP9 ARMBOARD
JP14 JP
JP16 JP15 JP 10
JP 20
JP17 18 JP11 JP12 JP13

JP 7 - home sensor of Z shift JP14 - home sensor of Y shift

JP 8 - main cable for connection to mainboard JP15 - home senzor of syringe
JP 9 - position sensor of the aspiration arm JP16 - level detection circuit
JP10 - three-way valve JP17 - barcode reader
JP11 - stepper motor of Y shift JP18 - LED lighting 1 and 2
JP12 - stepper motor of Z shift JP20 - fan of strip drying
JP13 - stepper motor of syringe

97 Rev. 3