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sistance for athletes while performing extension at the hip, uantification of muscle activation of lower body
knee, and plantar flexion at the ankle. This study evaluated the resistance training exercises allows practitioners
to make informed decisions regarding which
activation of the biceps femoris (BF), gluteus maximus (GMx),
exercises are optimal for performance enhance-
gluteus medius (GMe), rectus femoris, semitendinosus (ST),
ment and rehabilitation. A variety of muscles are active during
vastus lateralis, and vastus medialis during 4 variations of the
both dynamic sport movement and resistance training
step-up exercise to assess the specific muscle training exercises, including those that flex and extend the knee and
stimulus of each exercise variation. The exercises included hip, and those that abduct and adduct the leg at the hip,
the step-up, crossover step-up, diagonal step-up, and lateral including the hamstrings, gluteals, and quadriceps.
step-up. Fifteen women who regularly engaged in lower body Of these muscle groups, the hamstring muscle group has
resistance training performed the 4 exercises with 6 repe- been shown to be important in reducing anterior cruciate
tition maximum loads on a 45.72-cm (18-in.) plyometric box. ligament (ACL) injury risk, and evidence indicates training
Data were collected with a telemetered electromyography reduces hamstring inhibition and quadriceps to hamstrings
(EMG) system, and root mean square values were calculated ratio (11). Although there is a growing body of literature on
for EMG data for eccentric and concentric phases. Results of hamstring activation during resistance exercise and ham-
string to quadriceps ratios, other muscle groups within the
a repeated-measures analysis of variance revealed a variety
hip complex have received less attention. For instance, few
of differences in muscle activation between the exercises
have examined the eccentric and concentric phases (39) or
(p # 0.05). The results indicated that the crossover step-up
the role of the gluteus medius (GMe) in closed chain
elicited the greatest concentric muscle activation for the resistance exercise (1,14,38).
GMe, whereas the step-up elicited greatest eccentric Although data have indicated reduced activation of gluteus
activation for the GMe and greatest activation for the GMx, maximus (GMx) during single-leg activities (40), little data
BF, and ST in both concentric and eccentric phases. These exist to describe the role of the GMe. Studies examining the
findings can be used by practitioners to inform exercise role of the GMe during dynamic movements such as jump
landings and cuts have shown strengthening the GMe may
reduce the risk of ACL rupture through reduction in dynamic
valgus position (6,7,19). Training the GMe may improve both
Address correspondence to Dr. Christopher J. Simenz, christopher. strength and timing of GMe activation, which may reduce
simenz@mu.edu. dynamic knee valgus during sport and exercise, reducing risk
26(12)/3398–3405 of ACL injury (6,7,21,26,28,31,41).
Journal of Strength and Conditioning Research The literature has shown the benefits of including loaded
Ó 2012 National Strength and Conditioning Association single-leg exercises to improve functional stability, allowing
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TABLE 2. RMS EMG data for the BF, expressed as a percentage of MVIC for each 6RM during eccentric and concentric
phases of 4 step-up variations (N = 14).*
Eccentric phase Step-up† Crossover step-up‡ Diagonal step-up‡ Lateral step-Up‡§k
26.92 6 12.35 25.73 6 11.94 21.46 6 10.93 15.73 6 5.76
Concentric phase Step-up Diagonal step-up Crossover step-up Lateral step-up
73.14 6 28.64 69.94 6 28.49 64.24 6 33.57 55.44 6 18.00
*RMS = root mean square; EMG = electromyography; BF = biceps femoris; MVIC = maximal voluntary isometric contraction;
RM = repetition maximum.
†Significantly different from lateral step-up (p # 0.05).
‡Significantly different from diagonal step-up (p # 0.05).
§Significantly different from step-up (p # 0.05).
kSignificantly different from crossover step-up (p # 0.05).
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Variations of the Loaded Step-Up Exercise
TABLE 3. RMS EMG data for GMx, expressed as a percentage of MVIC for each 6RM during eccentric and concentric
phases of 4 step-up variations (N = 14).*
Eccentric phase Step-up†‡ Diagonal step-up Crossover step-up Lateral step-up
97.47 6 84.58 82.42 6 51.42 80.41 6 55.20 75.54 6 46.66
Concentric phase Step-up†‡§ Lateral step-up Diagonal step-up Crossover step-up
240.98 6 201.56 152.96 6 133.3 144.00 6 79.09 127.98 6 72.07
*RMS = root mean square; EMG = electromyography; GMx = gluteus maximus; RM = repetition maximum; MVIC = maximal voluntary
isometric contraction.
†Significantly different from lateral step-up (p # 0.05).
‡Significantly different from crossover step-up (p # 0.05).
§Significantly different from diagonal step-up (p # 0.05).
vastus medialis (VM), expressed as a percentage of maximal normalize the EMG data. The subjects also received
voluntary isometric contraction (MVIC). instruction in and performed the 4 exercises including the
step-up, crossover step-up, diagonal step-up, and lateral
Subjects
step-up. These exercises were selected for evaluation
Fifteen women (mean 6 SD; age 21.0 6 1.41 years; body
because they all are characterized by hip and knee extension,
mass 63.56 6 6.89 kg, height 159.84 6 28.99 cm) volunteer
and diagonal, lateral, and crossover step-up are additionally
university students who regularly engaged in lower body
characterized by hip abduction and adduction in a dynamic,
resistance training for at least 1 year before participation in
single-leg fashion, which is thought to elicit greater GMe
the study, and participated in sports ranging from club level
activation (24). The subjects were then tested to determine
to professional soccer, served as subjects. Subject descriptive
their 6RM for each step-up variation. Six RM loads were
information including training experience and status are
chosen because this study sought to test muscle strength as
given in Table 1. The study was approved by the institution’s
opposed to muscle endurance (16), and this exercise
university internal review board. All the subjects provided
intensity was previously used in studies assessing lower
informed consent.
body muscle activation (10,11). Approximately 72 hours
Procedures after the habituation session, the subjects returned for the
All the subjects performed a habituation and testing session. testing session.
Before each session, the subject performed a general warm- During the testing session, the subjects performed the
up including 5 minutes on an ergometer and a dynamic same dynamic warm-up session as in the habituation
warm-up for each of the major muscle groups to be used session, followed by 5 minutes of rest. The subjects then
in the test exercises. During the habituation session, all performed 2 repetitions of each of the step-up test exercises
the subjects were familiarized with the test procedures, in a randomized order with 6RM load, with 5 minutes of rest
including performing MVIC, which were obtained to between each exercise. For each step-up variation, EMG
TABLE 4. RMS EMG data for GMe, expressed as a percentage of MVIC for each 6RM during eccentric and concentric
phases of 4 step-up variations (N = 14).*
Eccentric phase Step-up†‡ Diagonal step-up‡§k Crossover step-up†‡ Lateral step-up†§k
41.90 6 15.04 40.41 6 14.80 38.39 6 15.66 36.46 6 15.10
Concentric phase Crossover step-up†‡ Step-up†‡ Diagonal step-up‡§k Lateral step-up†‡k
76.47 6 23.40 69.57 6 16.75 65.87 6 15.73 54.66 6 13.77
*RMS = root mean square; EMG = electromyography; MVIC = maximal voluntary isometric contraction; GMx = gluteus maximus;
RM = repetition maximum.
†Significantly different from diagonal step-up (p # 0.05).
‡Significantly different from lateral step-up (p # 0.05).
§Significantly different from step-up (p # 0.05).
kSignificantly different from crossover step-up (p # 0.05).
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TABLE 5. RMS EMG data for RF, expressed as a percentage of MVIC for each 6RM during eccentric and concentric
phases of 4 step-up variations (N = 14).*
Eccentric phase Diagonal step-up†‡ Lateral step-up†‡ Step-up§k Crossover step-up§k
41.59 6 10.49 39.93 6 13.07 35.69 6 9.24 35.40 6 10.93
Concentric phase Lateral step-up Diagonal step-up Crossover step-up Step-up
62.72 6 18.51 62.56 6 19.92 59.50 6 15.30 57.16 6 16.94
*RMS = root mean square; EMG = electromyography; MVIC = maximal voluntary isometric contraction; RF = rectus femoris; RM =
repetition maximum.
†Significantly different from step-up (p # 0.05).
‡Significantly different from crossover step-up (p # 0.05).
§Significantly different from diagonal step-up (p # 0.05).
kSignificantly different from lateral step-up (p # 0.05).
data were collected from the BF, GMx, GMe, RF, ST, VL, Procedures—Step-Up
and VM. Randomization and adequate rest between sets was All step-up exercises were performed on a 45.72-cm (18.0-in.)
used to reduce order effects and potential fatigue consistent plyometric box. This box height was selected to provide
with previous studies (10,11). For each exercise, the right a challenging step-up training stimulus, consistent with box
foot was identified as the lead (load bearing) foot. All EMG heights that are believed to be used in strength training
data were collected from the muscles in the right leg. The programs and similar to those used in previous research
technique for each step-up exercise variation is described as examining muscle activation during lower body resistance
follows. training exercises (11).
The subject stepped with the posterior border of the lead
Procedures—Maximum Voluntary Isometric Contraction
leg heel landing flush with the leading edge of the step box
Maximum voluntary isometric contractions for the BF and ST
and with heel-to-toe foot position perpendicular to the
groups were measured at 60° of knee flexion using the seated
leading edge of the box. The starting position was
leg curl (Hammer Strength, Schiller Park, IL, USA).
characterized by the trail leg in 10° hyperextension at the
Maximum voluntary isometric contractions for the VL,
hip measured from the greater trochanter to the midline of the
VM, and RF were measures at 60° of knee flexion on the
femur. The subject then extended the knee and hip of the lead
leg extension machine (Magnum Fitness Systems, South
leg until the trail foot was placed on the box lateral to the lead
Milwaukee, WI, USA). Maximum voluntary isometric
foot. The trail foot then returned to starting position, and the
contractions for the GMx was measured with subject lying
process was repeated.
prone at approximately 70° hip flexion on a decline bench for
the (Magnum Fitness Systems), and GMe was tested with the Procedures—Crossover Step-Up
subject’s leg abducted to approximately 25° against a padded, The subject started to the right of the box, with toes of the trail
immovable mass. foot flush with the leading edge of the box. The lead foot was
TABLE 6. RMS EMG data for ST, expressed as a percentage of MVIC for each 6RM during eccentric and concentric
phases of 4 step-up variations (N = 14).*
Eccentric phase Step-up†‡ Diagonal step-up†‡ Crossover step-up§k Lateral step-up§k
28.80 6 12.35 23.81 6 12.26 21.72 6 10.0 16.81 6 9.16
Concentric phase Step-up Diagonal step-up Crossover step-up Lateral step-up
57.36 6 29.34 52.50 6 26.0 41.85 6 20.73 42.91 6 24.51
*RMS = root mean square; EMG = electromyography; ST = semitendinosus; MVIC = maximal voluntary isometric contraction; RM =
repetition maximum.
†Significantly different from crossover step-up (p # 0.05).
‡Significantly different from lateral step-up (p # 0.05).
§Significantly different from step-up (p # 0.05).
kSignificantly different from diagonal step-up (p # 0.05).
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Variations of the Loaded Step-Up Exercise
TABLE 7. RMS EMG data for VL, expressed as a percentage of MVIC for each 6RM during eccentric and concentric
phases of 4 step-up variations (N = 14).*
Eccentric phase Step-up Diagonal step-up† Crossover step-up Lateral step-up‡
61.76 6 29.51 56.62 6 14.64 54.74 6 21.72 51.66 6 15.57
Concentric phase Diagonal step-up Crossover step-up Step-up Lateral step-up
99.37 6 29.31 97.50 6 29.20 95.02 6 26.29 94.25 6 26.31
*RMS = root mean square; EMG = electromyography; VL = vastus lateralis; MVIC = maximal voluntary isometric contraction; RM =
repetition maximum.
†Significantly different from lateral step-up (p # 0.05).
‡Significantly different from diagonal step-up (p # 0.05).
placed onto the corner of the box, with the posterior border of Procedures—Lateral Step-Up
the heel flush with the leading edge and the lateral aspect of The subject started to the left of the box with lead foot on the
the foot flush with the lateral edge of the box. The subject box. Medial edge of lead foot was placed 6 in. from the left
distance from box was determined by the measurement of edge of the box with the posterior border of the heel flush
shin angle in the frontal plane of 35° from the vertical. The with the leading edge of the box. The lead leg started with
subject then extended the knee and hip of the lead leg, a 35° shin angle from the vertical in the frontal plane. The lead
accompanied by hip abduction at the right leg until the trail leg started in neutral anatomical position. The subject then
foot landed on the step box directly lateral to the lead foot. extended the knee and hip of the lead leg until the trail foot
The trail foot was then returned to the starting point and the touched the box directly lateral to the lead foot. The trail foot
process repeated. then returned to starting position and the process repeated.
TABLE 8. RMS EMG data for VM, expressed as a percentage of MVIC for each 6RM during eccentric and concentric
phases of 4 step-up variations (N = 14).*
Eccentric phase Diagonal step-up Crossover step-up Step-up Lateral step-up
62.51 6 14.51 62.14 6 23.17 60.48 6 14.68 57.55 6 15.72
Concentric phase Diagonal step-up Crossover step-up Lateral step-up Step-up
106.89 6 30.02 105.47 6 29.10 102.73 6 24.28 103.30 6 28.23
*RMS = root mean square; EMG = electromyography; MVIC = maximal voluntary isometric contraction; RM = repetition maximum;
VM = vastus medialis.
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one-fifth of the distance from the medial joint line to the GMx, RF, ST, VL, VM. Tables 2–8 present the specific data
anterior superior iliac spine. The LH electrode was placed for each of the muscle group and step-up variation.
halfway between the ischial tuberosity and the fibular
insertion site, at least 5 cm proximal to the musculotendinous DISCUSSION
junction. The MH electrode was placed halfway between the This is the first study to comprehensively evaluate a variety of
ischial tuberosity and the tibial insertion point, at least 4 cm step-up exercises using training loads based on RM testing
proximal to the musculotendinous junction. The GMx while monitoring the activation of the GMe musculature
electrode was placed on the muscle belly one-third of the along with a large number of other hip and thigh musculature.
distance from the second sacral spine to the greater Significant differences were found between step-up variations
trochanter. The GMe electrode was placed one-third of the and between concentric and eccentric phases for the GMe,
distance from the iliac crest to the greater trochanter. A contrary to the findings of Ayotte et al. (1) who found no
common reference electrode was placed 10 mm anterior and significant differences in GMe activation between the step-up
halfway between the medial condyle and medial malleolus of and lateral step-up exercises in unloaded subjects. Thus, in
the tibia. Skin preparation included shaving hair if necessary, higher load testing conditions such as the 6RM used in this
abrasion, and cleaning the surface with alcohol. Elastic tape study, GMe activation appears to change as a function of
was applied to ensure electrode placement to minimize exercise variation. Specifically, the crossover step-up was
motion artifact and to provide strain relief for the electrode found to elicit the greatest concentric activation of the GMe,
cables. Surface electrodes were connected to an amplifier and whereas the step-up produced the greatest eccentric
streamed continuously through an analog to digital converter activation, which may be because of the starting position
(DelSys Inc.) to an IBM-compatible notebook computer. of crossover step-up, which placed the lead leg of the subject
into femoral adduction. As a result, GMe showed greater
Statistical Analyses activation during the concentric phase of the crossover step-
All data were filtered with a bandpass filter allowing 10 Hz up, as the position likely forced the muscle to activate in an
high pass and 450 Hz low pass, saved, and analyzed with the attempt to abduct the femur. This finding suggests the
use of computer software (EMGworks 3.1, Delsys, Inc.). Root crossover step-up should be included in resistance training
mean square EMG-signal processing was calculated over programs for court and field sport athletes in an attempt to
a 125-millisecond moving window and used on all EMG data reduce incidence of dynamic knee valgus, as weakness/
for the duration of the exercise and normalized to the RMS fatigue in the GMe has been shown to contribute to this (6,7).
EMG signal of the MVIC to determine muscle activation and Thus, training of the GMe in this fashion should be
to evaluate results with respect to previous research. Data incorporated to reduce dynamic valgus, a common injury
were analyzed for the second repetition of each exercise and position because of unplanned changes of direction and
compared with the MVIC of each muscle group. Data were cutting maneuvers, because the GMe plays a role in dynamic
analyzed for seconds 2–3 of the MVICs, using the highest of pelvic stabilization and the reduction of dynamic valgus of
the trials. the knee during such maneuvers (18).
The statistical analyses were undertaken with SPSS 17.0. In this study, all muscle groups exhibited concentric
A 2-way mixed analysis of variance with repeated measures activations that were much greater than eccentric, consistent
for step-up exercise type was used to evaluate the main with the findings of previous research (15,34). The GMx
effects for step-up variation and the interaction between showed significantly different activation patterns between
step-up variation and eccentric-concentric phase, for RMS exercises. The greatest activation for both concentric and
EMG of each muscle groups. When the main effects were eccentric phases was elicited by the step-up exercise, which is
found, Bonferroni adjusted pairwise comparisons were consistent with the muscle’s predominantly inferior-superior
used to identify the specific differences in muscle activation line of pull (29). Much greater activation was demonstrated
for each exercise. Assumptions for linearity of statistics for this exercise than has been previously shown during the
were tested and met. An a priori alpha level of p # 0.05 was loaded squat exercise when using 6RM loads (33).
used. In this study, the RF showed greatest activation during the
lateral step-up, which ranged from 39.93% of MVIC during
RESULTS the eccentric phase to 62.72% during the concentric phase
The analysis of EMG data revealed significant main effects and diagonal step-up exercises, which ranged from 41.59%
(p # 0.001) for BF, GMx, GMe, RF, ST, and VL, indicating MVIC during the eccentric phase to 62.56% during the
that there were differences in activation of these muscle concentric phase, both of which were performed with 6RM
groups between the step-up exercise variations. No differ- intensity. However, both were completed with lighter
ences were found for the VM (p = 0.833). A significant absolute loads compared with the step-up and crossover
interaction (p # 0.001) was found for exercise type and phase step-up.
for GMe. Analysis revealed no significant interactions The VL and VM showed no significant differences
between exercise type and phase (p # 0.05) for the BF, between concentric and eccentric phases, contrary to
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Variations of the Loaded Step-Up Exercise
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