Scribd Logo
Upload

Welcome to Scribd!

  • 14 views

    Semester Test 1 Without Memo-2

    Uploaded by

    Lencelot Malope
    The document provides a university semester test with 5 questions covering various topics in microbiology and biochemistry. The test includes questions about: 1) Preparing a 500 mL 100 mM phosphate buffer solution at pH 7.0 from available solutions of phosphoric acid and its conjugate bases. 2) Drawing the detailed structure of the peptide Gly-Phe-Asp-Gln at pH 7.0 including all atoms and charges. 3) Briefly explaining the meaning of "primary", "secondary", "tertiary", and "quaternary" protein structure. 4) Identifying which of four 3D protein structure representations is most likely that of myoglobin based on structural features.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Semester Test 1 Without Memo-2

    Uploaded by

    Lencelot Malope
    14 views1 page
    The document provides a university semester test with 5 questions covering various topics in microbiology and biochemistry. The test includes questions about: 1) Preparing a 500 mL 100 mM phosphate buffer solution at pH 7.0 from available solutions of phosphoric acid and its conjugate bases. 2) Drawing the detailed structure of the peptide Gly-Phe-Asp-Gln at pH 7.0 including all atoms and charges. 3) Briefly explaining the meaning of "primary", "secondary", "tertiary", and "quaternary" protein structure. 4) Identifying which of four 3D protein structure representations is most likely that of myoglobin based on structural features.

    Original Description:

    test 2 biochem

    Original Title

    Semester Test 1 without memo-2

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document provides a university semester test with 5 questions covering various topics in microbiology and biochemistry. The test includes questions about: 1) Preparing a 500 mL 100 mM phosphate buffer solution at pH 7.0 from available solutions of phosphoric acid and its conjugate bases. 2) Drawing the detailed structure of the peptide Gly-Phe-Asp-Gln at pH 7.0 including all atoms and charges. 3) Briefly explaining the meaning of "primary", "secondary", "tertiary", and "quaternary" protein structure. 4) Identifying which of four 3D protein structure representations is most likely that of myoglobin based on structural features.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    14 views1 page

    Semester Test 1 Without Memo-2

    Uploaded by

    Lencelot Malope
    The document provides a university semester test with 5 questions covering various topics in microbiology and biochemistry. The test includes questions about: 1) Preparing a 500 mL 100 mM phosphate buffer solution at pH 7.0 from available solutions of phosphoric acid and its conjugate bases. 2) Drawing the detailed structure of the peptide Gly-Phe-Asp-Gln at pH 7.0 including all atoms and charges. 3) Briefly explaining the meaning of "primary", "secondary", "tertiary", and "quaternary" protein structure. 4) Identifying which of four 3D protein structure representations is most likely that of myoglobin based on structural features.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 1

    UNIVERSITY OF THE FREE STATE Question 2 [5]

    You have available 1 M solutions of H3PO4 (pKa 2.15), H2PO4- (pKa 6,82) and HPO42- (pKa
    MAIN CAMPUS 12,38). Explain how you will prepare 500 ml of a 100 mM phosphate buffer at pH 7,0. Show all
    calculations.   
    BOCB2616
    Question 3 [5]
    DEPARTMENT OF MICROBIOLOGY AND BIOCHEMISTRY Draw the detailed structure of the peptide Gly-Phe-Asp-Gln (at pH 7,0). Show all atoms and
    charges.
    Semester Test 1: 7 April 2022
    Question 4 [5]
    ASSESSOR: Prof. M.S. Smit Briefly explain what is meant by the “primary”, “secondary”, “tertiary” and “quaternary” structure of
    MODERATOR: Prof. H.G. O’Neill proteins.

    TIME: 1 hour MARKS: 50 Question 5 [5]


    The figure below shows three-dimensional representations of four different proteins. Different
    coloured (shades of grey) cartoons indicate different polypeptide chains. Which of these (A, B, C
    Supplementary information or D) is most likely a representation of the structure of myoglobin? Explain your answer. Why
    could the others not be myoglobin?

    PART A [25 marks]

    Online test to be completed in 30 min.


    PART B [25 marks]
    ANSWER ALL QUESTIONS ON TEST PAPERS. REMEMBER TO ENTER YOUR STUDENT NUMBER!

    Question 1 [5]
    A. Explain why the melting point of methane (CH4) (-182,5 ⁰C) is so much lower than that of ice
    (H2O) (0 ⁰C), while their molecular weights (16 and 18) are very similar. (3)  
    B. Explain what happens when fatty acids are mixed with water. (2)

    You might also like