Analytical HPLC

LC-4000 Series

Performance
Innovation
Reliability
The LC-4000 Series HPLC is the latest in a long
history of innovative HPLC systems developed by
JASCO reaching all the way back to the start of
the commercial HPLC in the early 1970s.

The concept of the integrated LC-4000 series

HPLC provides key separation platforms at 30 MPa,
70 MPa and 130 MPa which correspond to
conventional HPLC, the increasingly popular
Rapid Analysis (RHPLC) and sub 2 µm UHPLC,
respectively. Each platform is supplied with a
dedicated pump and autosampler matched to the
operating pressure and all three platforms share
common detectors optimized for high-speed
100 Hz acquisition and narrow peak shapes
common to both RHPLC and UHPLC.

Table of contents
System
LC-4000 Advances 3
System Configurations 4
Detector Options 5
Software
ChromNAV 6
Specifications 8

2
LC-4000 Advances
Flow Innovation 200000

For over two decades JASCO analytical HPLC

pumps have employed an asymmetric twin-piston
delivery system SSQD (Slow Suction, Quick Delivery)
providing significantly better flow and pressure

Intensity
100000
profiles than conventional twin-piston reciprocating
designs. The SSQD was redeveloped for the LC-
4000 series to offer the highest stability in solvent
delivery using ExReFT (Extremely Reliable Flow
Technology). 0

0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0

Retention time [min.]
Flow rate accuracy of PU-4180 (1.0 mL/min., n = 10)
Peak retention time reproducibility of PU-4180 (n = 10)

Retention Time Naphthalene Fluorene Anthracene Pyrene Chrysene Benzo[a]pyrene

% RSD 0.025 0.018 0.017 0.022 0.016 0.020

Pioneering Optical Design

As a pioneer in optical spectroscopy dating back over 65 years, JASCO has been at the forefront of optical
detection. Adapting designs from the most powerful spectrometers, JASCO has developed a range of HPLC
detectors with unrivaled performance like the class leading FP-4020 fluorescence detector with S/N of
over 2300:1 and the world’s only circular dichroism detector for chiral chromatography. Dual simultaneous
wavelength detection is offered as standard on the UV and FP detectors adding flexibility and versatility. In
addition, JASCO offers RI (refractive index), PDA, OR (optical rotation), and MS detectors.
100000
Semi-micro cell
S/N 20 % UP 3421 Analytical cell
Anthracene: 100 fg injection *Value on the peak is S/N.
20000 2744

2854
S/N 10 % UP
Intensity

50000 2283 1708

Intensity

1415
10000
1582
1253

0
0
0.0 1.0 2.0 3.0 4.0 5.0 0.0 1.0 2.0
Retention time [min.] Retention time [min.]

High sensitivity detection of FP-4020 Redesigned flow cells for excellent peak shape.

Compact and Easy to Use

Despite the extra power delivered by the LC-4000 series HPLC, the
standard footprint is only 300 mm wide requiring very little bench
space. For those users that require front panel control, the LC-4000
series returns the popular keypad and display.

For easy user maintenance, all LC-4000 modules feature front access
for replacing consumables such as check valves and seals in the pumps,
sample needle and syringe parts in the autosamplers and lamps for the
detectors.

3
Configurations
HPLC
Designed for routine HPLC research and academic settings.
For use with 5 µm columns.

Flow rate: 0.500 to 10.0 mL/min.

(Semi-micro option 0.050 to 4.0 mL/min.)
Pressure: Analytical (0.500 to 10.0 mL/min.): 35 MPa
Semi-micro (0.050 to 4.0 mL/min.): 40 MPa
Inert system: 25 MPa
Options:
• Isocratic
• High Pressure Gradient
• Low Pressure Gradient
• Autosampler up to 100 µL injections standard
Optional 1 mL injection and temperature control
• Column Oven for various column lengths
• UV, PDA, FP, RI, CD, OR, MS

RHPLC
Designed for those requiring more sample throughput. For use with 2.5 µm
coreshell, 3 µm and 5 µm columns.

Flow rate: 0.500 to 6.0 mL/min.

(Semi-micro option 0.050 to 3.0 mL/min.)
Pressure: 70 MPa
Options:
• Isocratic
• High Pressure Gradient
• Low Pressure Gradient
• Autosampler up to 20 µL injections standard
Optional 1 mL injection and temperature control
• Column Oven for various column lengths
• Detection: UV, PDA, FP, RI, CD, OR, MS

UHPLC
Designed for those requiring the highest sample throughput. For use with sub-2 µm columns.

Flow rate: 0.050 to 2.0 mL/min.

Pressure: 0.050 to 2.0 mL/min.: 100 MPa
0.050 to 1.5 mL/min.: 130 MPa
Options:
• Isocratic
• High Pressure Gradient
• Autosampler up to 5 µL injections standard
Optional 1 mL injection and temperature control
• Column Oven for various column lengths
• Detection: UV, PDA, FP, RI, CD, OR, MS

4
 Detectors

UV-4070/4075 MD-4010/4015/4017 CD-4095 CMS

UV-Visible Detector UV-Visible PDA Detectors Circular Dichroism Mass Spectrometer
Both detectors offer When 2 wavelengths are Detector When mass identification is
simultaneous dual not enough a PDA can The world’s only circular needed the CMS offers ESI,
wavelength acquisition and provide the additional dichroism detector that APCI or ASAP with positive
spectra scanning. needed as well as provides the utmost and negative switching.
spectral information and sensitivity.
Wavelength ranges: identification possibilities. CMS-S up to 1200 m/z
UV-4070: 190 to 900 nm Wavelength range: CMS-L up to 2000 m/z
Wavelength ranges: 220 to 460 nm
UV-4075: 190 to 600 nm
MD-4010: 190 to 900 nm
MD-4015: 200 to 600 nm
MD-4017: 200 to 400 nm

FP-4020/4025 OR-4090 RI-4030/4035

Fluorescence Detector Optical Rotation Detector Refractive Index Detector
For the ultimate in sensitivity the The optical rotation detector The refractive index
FP-4020 provides S/N of 2300:1. provides chiral detection for detector is a universal
The FP-4025 offers excellent optically active isomers and detector for those
sensitivity with S/N of 1400:1 and chiral compounds that have compounds that cannot be
both offer simultaneous detection no absorption. seen on the UV or FP.
of 2 wavelength pairs.
Light source: Hg/Xe lamp RI-4030 up to 120 mL/min.
Wavelength range: RI-4035 for RHPLC/UHPLC
200 to 700 nm

Related Instruments

LCMS Prep LCMS

5
ChromNAV Software
ChromNAV 2.0 (and ChromNAV-CFR 2.0) are JASCO’s next generation CDS developed from the powerful and
easy-to-use ChromNAV 1.0 with a host of existing new features. With a customizable graphical-user-interface
(GUI), the user can set-up the system to display only the functions necessary for their application. This latest
intuitive GUI allows the user to quickly learn the operation and explore the extensive functionality of data
processing.

ChromNAV 2.0 is a universal CDS which can be used with any type of separation – HPLC, RHPLC, UHPLC,
Prep LC, Analytical SFC and Prep SFC. ChromNAV can also satisfy the demands of dedicated analyses or
mutli-purpose systems.

Control Method
The pump flow rate and gradient profile display is
flexible and can be overlaid with a chromatogram for
adjusting gradient conditions.

PDA Analysis
PDA data processing is included as standard.
Data is displayed in a 2D contour plot and 3D with
simultaneous overlay of spectra and chromatograms.
Chromatograms can be extracted at single or multiple
wavelengths for quantitation.

ChromNAV 2.0 offers powerful system control and data acquisition. During acquisition, the run-time can
be extended to capture later eluting peaks. Previously acquired chromatograms can be overlaid for visual
comparison with data currently being acquired.

Samples can be changed or added to the sequence while it is acquiring. Also the sequence can be setup to
stop the pump, turn off the lamps, turn off the oven temperature and even turn off the power on the system at
the end of the sequence.

6
Standard Features
• Peak integration and peak identification
• Peak grouping
• Linear and non-linear quantitation
• 3D chromatogram analysis
• Spectral analysis for UV-visible, Fluorescence and PDA detectors
• Customizable report generator
• User formula calculations
• Automatic raw data export

All data is protected and saved; which can then be analyzed and re-analyzed, reported and saved with both
raw data and with any data processing from the user’s analysis.

A comprehensive audit trail records the acquisition method along with the history of the instrument performance
in each data file. This provides the user with a snap-shot of the condition of the system during the run and can
indicate warnings about requirements for impending maintenance.

Optional Applications
• ChromNAV CFR for CFR Part 11 compliance and electronic registration of data
• ChromNAV GPC/SEC for molecular weight dispersion calculations and determinations
• ChromNAV Herparin for molecular weight dispersion of low molecular weight heparin
• ChromNAV FUMI for Function of Mutual Information (FUMI) for theoretical precision analysis
• ChromNAV FC for fraction collection (included as standard with a Prep LC)
• ChromNAV CMS for control of the CMS Mass Spectrometer (included as standard with CMS)
• ChromNAV Method Scouting for solvent and column screening in SFC and HPLC

ChromNAV GPC/SEC
Molecular weight distribution program (Option) for
GPC and SEC analysis.

ChromNAV FC
Fraction collection control for the CHF-122SC fraction
collector triggered from time, threshold and/or slope.

7
Specifications
Pumps
Isocratic

System HPLC RHPLC UHPLC

0.500 to 10.0 mL/min. 0.500 to 6.0 mL/min.
Flow Range 0.050 to 2.0 mL/min.
(Semi-micro: 0.050 to 4.0 mL/min.) (Semi-micro: 0.050 to 3.0 mL/min.)
35 MPa
130 MPa (up to 1.5 mL/min.)
Maximum Pressure (Semi-micro: 40 MPa) 70 MPa
100 MPa (up to 2.0 mL/min.)
(Inert type: 25 MPa)
Flow Rate Accuracy ±1 % of setting value or ±2 µL/min., whichever is larger
Flow Rate Precision
Measured by 0.05 % RSD or ±0.04 min. SD, whichever is larger
Chromatogram
300 (W) × 470 (D) × 150 (H) mm, 300 (W) × 470 (D) × 150 (H) mm,
Dimensions, Weight
13.8 kg 15.3 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 115 VA AC 100 to 240 V, 50/60 Hz, 95 VA

Low Pressure Gradient

System HPLC RHPLC

Mixing Accuracy ±0.8 % (5 to 95 %, 0.5 to 5.0 mL/min.)
± 0.6 % (5 to 95 %, 0.2 to 1.0 mL/min.)
Semi-micro type
± 1.2 % (5 to 95 %, up to 4.0 mL/min.)
Mixing Precision
Measured by 0.25 % RSD or ±0.02 min. SD, whichever is larger
Chromatogram
Semi-micro type 0.15 % RSD or ±0.01 min. SD, whichever is larger
Dimensions, Weight 300 (W) × 470 (D) × 150 (H) mm, 16.0 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 115 VA

Binary High Pressure Gradient

System HPLC RHPLC UHPLC

±0.4 % ±0.4 %
Mixing Accuracy
(5 to 95 %, 0.2 to 4.0 mL/min.) (5 to 95 %, 0.2 to 2.0 mL/min.)

Mixing Precision
Measured by 0.15 % RSD or ±0.01 min. SD, whichever is larger
Chromatogram
300 (W) × 470 (D) × 150 (H) mm, 300 (W) × 470 (D) × 150 (H) mm,
Dimensions, Weight
21.9 kg 24.1 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 130 VA AC 100 to 240 V, 50/60 Hz, 110 VA

8
Autosamplers

Model AS-4050 (HPLC) AS-4150 (HPLC/RHPLC) AS-4250 (UHPLC)

Sample Injection
Full or partial fill loop injection
Method
Number of Samples 60 (2 mL vials) 180 (2 mL vials)
0.1 to 100.0 µL
Injection Volume
Large volume option (1 to 1000 µL)
Injection Accuracy ±0.1 % or less
Injection Precision 0.3 % RSD or less 0.25 % RSD or less
0.01 % or less
Carry Over 0.01 % or less
0.005 % or less with multiple solvent flushing
Maximum Pressure 30 MPa 70 MPa 130 MPa
Sample Cooling/ Option: (room temp. - 21) °C or 4 °C (whichever is higher)
Heating to 40 °C
Pre-Column
Standard (up to 2 reagents, dilution)
Derivatization
300 (W) × 470 (D) × 300 (H) mm, 300 (W) × 470 (D) × 385.5 (H) mm,
Dimensions, Weight
21 kg 25 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 60 VA AC 100 to 240 V, 50/60 Hz, 75 VA

Column Ovens

Model CO-4061 CO-4062 CO-4060 CO-4065

(Room temp. - 15) °C or 4 °C (Room temp. - 15) °C or 4 °C (Room temp. - 15) °C or 4 °C
Temperature Range (whichever is larger) (whichever is larger) (whichever is larger)
to 100 °C to 80 °C to 90 °C
Column 270 (W) × 30 (D) ×
260 (W) × 25 (D) × 110 (W) × 105 (D) × 260 (W) × 120 (D) ×
Compartment 60 (H) mm,
105 (H) mm 410 (H) mm 410 (H) mm
Dimensions Option 380 (W) mm
Safety Features Heating/cooling power shut off when unusually high temperatures or solvent leaks are detected
Optional Column Available Available
- -
Switching Valve (up to 6 positions) (up to 10 positions)
150 (W) × 470 (D) × 300 (W) × 470 (D) ×
Dimensions, Weight 300 (W) × 470 (D) × 150 (H) mm, 10 kg
465 (H) mm, 16 kg 465 (H) mm, 25 kg
AC 100 to 240 V, AC 100 to 240 V, AC 100 to 240 V, AC 100 to 240 V,
Power Requirement
50/60 Hz, 160 VA 50/60 Hz, 200 VA 50/60 Hz, 350 VA 50/60 Hz, 660 VA

Reaction Oven

Model RO-4068
Temperature Range (Room temp. + 10) °C to 200 °C
Reaction Coil Holder
Compartment 43 (W) × 354 (D) × 76 (H) mm
Dimensions
Heating/cooling power shut off when unusually high
Safety Features
temperatures or solvent leaks are detected
Dimensions, Weight 300 (W) × 470 (D) × 150 (H) mm, 12 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 450 VA
9
Specifications
Detectors
UV-Vis and Circular Dichroism

Model UV-4070 UV-4075 CD-4095

Light Source D2 lamp + WI lamp D2 lamp Hg-Xe lamp
Wavelength Range 190 to 900 nm 190 to 600 nm 220 to 460 nm
0.04 mdeg
Noise Level ±0.2 × 10-5 AU (specified condition)
(at specified conditions)
Drift
0.1 mdeg/h
At constant room 1 × 10-4 AU/h (specified condition)
(at specified conditions)
temperature
Data Output 100 Hz
Flow Cell Temperature controlled, tapered, path length 10 mm Tapered cell, path length 25 mm
Spectrum
200 to 900 nm 200 to 600 nm 220 to 460 nm
Measurement
300 (W) × 470 (D) × 150 (H) mm, 300 (W) × 470 (D) × 225 (H) mm,
Dimensions, Weight
14 kg 21 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 175 VA AC 100 to 240 V, 50/60 Hz, 125 VA AC 100 to 240 V, 50/60 Hz, 210 VA

Photo Diode Array

Model MD-4010 MD-4015 MD-4017

Light Source D2 lamp + WI lamp D2 lamp D2 lamp

Wavelength Range 190 to 900 nm 200 to 600 nm 200 to 400 nm

PDA Elements 1024 channels 512 channels 512 channels
Slit Width 1, 4, 8 nm 4 nm 4 nm
±3.0 × 10-6 AU ±7.0 × 10-6 AU
Noise Level
(specified condition) (specified condition)
±0.5 × 10-3 AU/h ±1.0 × 10-3 AU/h
Drift
(specified condition) (specified condition)
2.0 AU or more
Linearity
(specified condition)
Data Acquisition
100 spectra/sec. 20 spectra/sec.
Rate
Flow Cell Path length 10 mm
PC Communication USB
300 (W) × 470 (D) × 150 (H) mm, 300 (W) × 470 (D) × 150 (H) mm,
Dimensions, Weight
14.5 kg 13.5 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 180 VA AC 100 to 240 V, 50/60 Hz, 150 VA AC 100 to 240 V, 50/60 Hz, 120 VA

10
Fluorescence

Model FP-4020 FP-4025

Light Source Xenon short arc lamp
200 to 700 nm
Wavelength Range
Option up to 900 nm
Spectral Bandwidth Excitation side: 20 nm, Emission side: 20 or 40 nm
Sensitivity Raman peak of water S/N > 2300 Raman peak of water S/N > 1400
Data Output 100 Hz
Flow Cell Front loading cassette cell
Temperature
OFF, (room temp. - 10) °C to 40 °C -
Control
Spectrum
Excitation and emission spectrum measurement
Measurement
Two-Wavelength Two sets of Ex/Em wavelength setting.
Monitoring Maximum wavelength difference 200 mm or shorter.
Dimensions, Weight 300 (W) × 470 (D) × 225 (H) mm, 24 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 270 VA AC 100 to 240 V, 50/60 Hz, 230 VA

Refractive Index

Model RI-4030 RI-4035

Measurement
Deflection type
System

Refractive Index
1.00 to 1.75
Range
5.0 × 10-5 RIU (HIGH)
5.0 × 10-5 RIU (HIGH)
Linearity 5.0 × 10-4 RIU (STD)
5.0 × 10-4 RIU (STD)(H2O)
5.0 × 10-3 RIU (LOW)(H2O)
0.20 × 10-8 RIU or less 0.50 × 10-8 RIU or less
Noise Level
(specified condition) (specified condition)
Cell Capacity 10 µL 2.7 µL
Maximum Flow 10 mL/min. (Low flow tubing)
1.2 mL/min. (specified condition)
Range 120 mL/min. (High flow tubing)
0.1 MPa (Low flow tubing)
Maximum Pressure 0.1 MPa
0.3 MPa (High flow tubing)
Temperature
(Room temp. + 10) °C to (room temp. + 25) °C
Control
Dimensions, Weight 300 (W) × 470 (D) × 150 (H) mm, 15 kg
Power Requirement AC 100 to 240 V, 50/60 Hz, 80 VA

ChromNAV 2.0 and ChromNAV-CFR 2.0

Language English or Japanese

Windows 7 Professional 32/64 bit
Windows OS Windows 8.1 Professional 32/64 bit
Windows 10 Pro 32/64 bit
LC-4000, XLC-3000, LC-2000,
Controllable
some LC-1500 and some LC-900.
Hardware
Control up to 4 systems.
11
JASCO CORPORATION
2967-5, Ishikawa-machi, Hachioji-shi, Tokyo 192-8537 Japan
Tel: +81-42-649-5177 Fax:+81-42-646-4515 Web: www.jasco.co.jp
Japan
JASCO INTERNATIONAL CO., LTD.
11-10, Myojin-cho 1-chome, Hachioji-shi, Tokyo 192-0046, Japan
Tel: +81-42-649-3247 Fax: +81-42-649-3518 Web: www.jascoint.co.jp/english/
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The contents of this brochure are for reference and illustrative purposes only. Information, descriptions, and specifications in this
publication are subject to change without notice. JASCO assumes no responsibility and will not be liable for any errors or omissions
contained herein or for incidental, consequential damages or losses in connection with the furnishing, performance or use of this material.