Ronozyme p5000 (CT)

The story of an excellent product
created in a strong Alliance

told by:
Yu-Guo Sun (DSM) & Frank Hatzack (Novozymes)
Frank Hatzack – China visit 2005
A strong alliance of two world leaders
created an excellent product
World leader in World leader in

Enzymes! Animal Nutrition!
• R&D • Nutrition Expertise
• Production • Global Distribution
• Granulation • Global Marketing
FU- Frank Hatzack – China visit 2005 2

Why is so successful?
Because it is:
An excellent …in an excellent …with excellent

Phytase enzyme... granulate... bio-efficacy!

An excellent
Phytase enzyme

Peniophora Phytase is a 6-Phytase
Aspergillus
OPO32-
phytases
OPO32- 2
2- O3PO 4
3
2- O3PO 5 OPO32-
6
2- O3PO 1
Lily pollen phytase
Peniophora phytase

Peniophora Phytase is a FAST Enzyme!
NMR experiment measuring the number of P-groups released per minute
核磁共振试验显示每分钟释放出的磷的集团…
Peniophore lycii,
Peniophora wt
phytase NatuPhos
Aspergillus phytase
6 6
20 FYT/ml
P-groups released
5 5
20 FYT/ml
4 4
1 FYT/ml 1 FYT/ml
3 3
2 2
1 1
0 0
0 5 10 15 0 5 10 15
min min

The pH profile of RONOZYME P phytase
植酸酶的PH值
Phytase pH profiles
1.3
more acidic
1.0
pH optimum Relative activity
than Aspergillus 0.8 P.lycii
phytase!
0.5 A.ficuum
Chineese
0.3
0.0
3 4 5 6 7 8
pH
Solubility of phyate is zero above pH 6

because of Ca-phytate precipitation
pH＞6时，植酸的溶解性为零
因为钙与植酸结合成植酸钙Ca-phytate，
FU- 形成一种不溶性的沉淀。 7
Phytate degradation in the pig
植酸在猪消化道中的降解
pH 1.5 – 5.4
5 min – 18 hours
pH 6.5 – 7.5 pH 6.5 – 7.0
3 – 8 hours 2 – 24 hours
9Phytate solubility high
9pH OK for phytase
9long retention time •Phytate solubility low
•pH not optimal for phytase

The digestive tract of poultry
植酸酶在家禽消化道中的降解
pH 4 – 6.5
up to 2 h
pH 3.5 – 4.5
0.5 min – 2 min 9Phytase pH optimum
嗉囊
前胃 9Phytate solubility high
9(if pH < 6)
9Phytate solubility high 胰腺
9long retention time
9OK for phytase
•but short retention time
肌胃
pH 2.5 – 3.5
1 min – 10 min
pH 5.5 – 7.5
1–4h
泄殖腔
pH 6.5 – 7.0
•Not optimal for phytase
2–6h •Phytate solubility low
So: Peniophora phytase in is
1. is a very fast enzyme, faster than Aspergillus
2. with a more acidic pH optimum than Aspergillus
3. these features make it highly effective in the upper part

of the digestive tract

An excellent
Granulate

Why do you need a stable phytase?
Pellet feed 9Want to use for pelleting at 85C
9Want storage stability
Mash, 9Want storage stability for long time
Condensed
9Storage stability even more important
Premix 9Enzyme must be stable in presence of
minerals and other additives
No Dust & 9Want no losses during processing

Physical strength 9Excellent overall handling characteristics

CT – Coated Thermostable Granulate
Patented technology
Multi-layer coating Cellulose fibers
1. for pelleting stability for physical stability
2. enzyme release
3. dust-free
Enzyme stabilized in
400-800 microns mineral&carbohydrate
matrix
for enzyme stability
Small, defined particles
1. high particle number per gram
2. Excellent mixability
3. Excellent homogeneity in feed

1. Thermostability,
2. Enzyme Release

CT Coating has dual function
1. To protect the enzyme during pelleting
2. To allow release of enzyme activity in stomach

3. A dust-free granulate

is dust-free (< 1 mg)
200
150
mg
100
50
0
Ronozyme Phytase N Phytase P1 Phytase P2 Phytase C2 Phytase B
P5000 CT

Why is dust-free important?
1. Dust means losses of product during handling
2. Dusty products do not mix well
3. Dust is a potential health hazard

This is a high quality granulate!
10X 25X

See the point of coating?
Competitor
CT Granulates
Spray on
organic carrier
Enzyme totally
exposed!
-> low stability!

Dust measurement Phytase
Done with a Heubach Dust Meter
植酸酶粉尘检测的结果
RONOZYME P
Phytase P1 and F
Phytase N
5000 G

4. Excellent mixability and
homogenity in feed

What is the relation between particle size, particle
number and mixability?
What does this mean for the animal??

Small granulate size means
high particle number per gram
• particle size of 400 – 800 µm
35.0
30.0
25.0
% of particles
20.0
15.0
10.0
5.0
0.0
<180 180-250 250-300 300-355 355-425 425-500 500-600 600-710 710-850 > 850
particle size intervals [祄 ]
• Average particle number per gram is > 14000!

What does number of particles per gram mean
for mixability and for the starter chick?
每克产品中的颗粒数对小鸡料意味着什么？
• Phytase N has 5000 Granules/g. Dose is 100 g/ton feed:
¾ 500.000 Granules/ton feed-> 500 Granules/kg feed -> 10 Granules/20g feed
• RONOZYME P 5000 CT has 14.200 Granules /g, dose is 100 g/ton feed
¾ 1.420.000 Granules/ton feed -> 1.420 Granules/kg feed -> 28 Granules/20g feed
¾With RONOZYME P there is a 3 times higher probability that a
broiler chicken gets the amount of phytase it should get each day!

So, if competitors dose at only 50 g per ton…
如果有些产品每吨只有50克添加量…
…and their product is a powder-form phytase with

heterogeneous particle size distribution…
…how do you think the broiler chicken is feeling?
will I get my
phytase granules
today?

Excellent mixability in a broiler premix
肉鸡预混料中卓越的混合均匀度
120
Enzyme content in feed (% of
100
80
average)
60
40
Coefficient of variation = 6.9%
20
变异系数 = 6.9%
0
Samples
VFP 21033B

Excellent mixability in a broiler feed
肉鸡饲料中的优异混合均匀度
140
Enzyme content in feed (% of
120
100
average)
80
60 Coefficient of variation = 9.2%
40 变异系数 = 9.2%
20
0
Samples
VFP 21033A
Excellent mixability in a swine premix
猪的预混料中有非常好的混合均匀度
120
Enzyme content in premix (% of average
100
80
60
Coefficient of variation = 5.9%
40 变异系数 = 5.9%
20
0
Samples
VFP 21033B
5. Excellent Pelleting
Stability

Excellent pelleting stability even at 95ºC
95ºC制粒时的卓越稳定性
100
conditioning time 30 sec
95
CV: 8.5%
CV: 3.3%
90 88%
85%
Recovery [%]
85
80
75
70
65
60
RO NO ZYM E P RO NO ZYM E P5000
Luna No.: 2004-17531-01 / TKv, PEJ
•3 different batches of each product
•tested independently in 2-3 runs
Has a much higher pelleting stability than local competitor
与竞争对手在制粒稳定性上的比较
95
100 93 89
Retention, (% mash)
80
60
43
40
20 15 13
0
70癈 at 80 癈 at 90 癈
conditioning time 30 sec

Ronozyme P5000 K24014
trial VFP24026

Has better storage stability in pelleted feed
than other phytase products
Recovery (% of activity in initial pelleted
100 30 secs conditioning

85 84
81
77
80
67
60 56
feed)
50
47
39
40 33
21 21
20
0
RONOZYME P 5000 Phytase N Phytase F
(CT)
Initial pellets 1 month @ R.T. 1 month @ 35 deg. C 3 months @ R.T.
VFP 21052
Comparative pelleting trial from Denmark Kolding
City Biotech Institute, 2005
120.0
RONOZYME P5000 30 sec conditioning
RONOZYME P (old product)
100.0
Competitor 1
Competitor 2
80.0
60.0
40.0
20.0
0.0
75C 75C 85C 85C 90C 90C 95C 95C
75C 75C 85C 85C 90C P CT P 5000 CT Competitor 1

90C
Competitor 2
95C 95C

Is the most pelletstable phytase in the world!

6. Biological Efficacy

Biological efficacy in broilers
Growth Performance (d. 1 – 36)
肉鸡的生物学活性生长成绩（d. 1 – 36 ）
2200
2107 2110
2100
W eight gain (g/bird)
2000
1900
1796
1800
1700
1600
Control RONOZYME P (CT) RONOZYME P5000
(CT)
Mean values in a row with different superscripts were significantly different: a-b (P < 0.05)
broiler trial (ME08/02)

FCR (d. 1 – 36)
肉鸡的生物学活性饲料转化率（ d. 1 – 36 ）
1.62
1.61
1.6
FCR (g feed / g gain)
1.6
1.589
1.59 1.583
1.58
1.57
1.56
1.55
(CT)
Mean values in a row with different superscripts were significantly different: a-b (P < 0.05)

Bone Ash ( % )
肉鸡的生物学活性骨骼灰份( % )
53 52.5
52.3
Bone Ash (%)
51
49.2
49
47
(CT)

Biological efficacy in pigs
Phosphorus digestibility ( % )
猪的生物学活性磷的消化率( % )
45
41.7
Phosphorus digestibility
40
37.1
(% of intake)
35
30
25 22.9
20
(CT)
pig trial (1008644)

Calcium digestibility ( % )
猪的生物学活性钙的消化率
65 64.1
Calcium digestibility
60
(% of intake)
56.5
55
49.5
50
45
(CT)
pig trial (1008644)

Growth performance ( d. 0 – 30) g/day
猪的生物学活性生长成绩( d. 0 – 30) g/day
800 794
789
Daily mean gain (g/day)
780
760
740
720
720
700
(CT)
pig trial (1008644)

FCR (d. 0 – 30 ) kg/kg
猪的生物学活性饲料转化率 (d. 0 – 30 ) kg/kg
2.91
2.9
FCR (Kg / Kg)
2.8
2.74
2.7
2.64
2.6
(CT)
pig trial (1008644)

Bone strength
(external metacarpal + metatarsal combined)
猪的生物学活性骨骼强度（外掌骨+）
280
263
Breaking strength (N)
236
240
202
200
160
(CT)
pig trial (1008644)

Growing pigs:
Ileal apparent digestibility improvements
生长猪回肠表观消化率的改善
total P* + 38.4% Asp + 2.9% Leu + 1.4% Thr + 5.7%
total IPP + 41.4% Cys + 1.8% Lys + 2.7% Tyr + 4.7%
Ca + 30.6% Glu + 1.9% Met + 4.3% Val + 3.5 %
Energy + 1.3% Gly + 8.4% Orn + 6.2%
Total N + 3.7% His + 2.1% Phe + 2.1%
Ala + 4.4% Hyp + 32% Pro + 1.8%
Arg + 2.2% Ile +2.3% Ser + 2.6%
RONOZYME P (CT) at 750 FYT/kg. Eight pigs at 60 days (WhitexLandracexPietrain). Ileal-rectal

anastomosis. Maize/SBM/barley diet fed ad libitum. 0.47 % P in diet. 5 periods of one week, 3
adaptation days, 4 ileal content sampling days. S06-2001.
*measured as total digestibility

This is why is so
successful!
Because it is:
An excellent …in an excellent …with excellent

Phytase enzyme... granulate... bio-efficacy!

Ronozyme p5000 (CT)

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The story of an excellent product

created in a strong Alliance

World leader in World leader in

FU- Frank Hatzack – China visit 2005 2

An excellent …in an excellent …with excellent

FU- Frank Hatzack – China visit 2005 3

FU- Frank Hatzack – China visit 2005 4

FU- Frank Hatzack – China visit 2005 5

FU- Frank Hatzack – China visit 2005 6

Solubility of phyate is zero above pH 6

FU- Frank Hatzack – China visit 2005 8

2. with a more acidic pH optimum than Aspergillus

3. these features make it highly effective in the upper part

FU- Frank Hatzack – China visit 2005 10

FU- Frank Hatzack – China visit 2005 11

No Dust & 9Want no losses during processing

FU- Frank Hatzack – China visit 2005 12

FU- Frank Hatzack – China visit 2005 13

FU- Frank Hatzack – China visit 2005 14

1. To protect the enzyme during pelleting

2. To allow release of enzyme activity in stomach

FU- Frank Hatzack – China visit 2005 15

FU- Frank Hatzack – China visit 2005 16

FU- Frank Hatzack – China visit 2005 17

1. Dust means losses of product during handling

2. Dusty products do not mix well

3. Dust is a potential health hazard

FU- Frank Hatzack – China visit 2005 18

FU- Frank Hatzack – China visit 2005 19

-> low stability!

FU- Frank Hatzack – China visit 2005 20

FU- Frank Hatzack – China visit 2005 21

FU- Frank Hatzack – China visit 2005 22

What does this mean for the animal??

FU- Frank Hatzack – China visit 2005 23

particle size intervals [祄 ]

• Average particle number per gram is > 14000!

FU- Frank Hatzack – China visit 2005 24

¾With RONOZYME P there is a 3 times higher probability that a

FU- Frank Hatzack – China visit 2005 25

…and their product is a powder-form phytase with

…how do you think the broiler chicken is feeling?

FU- Frank Hatzack – China visit 2005 26

FU- Frank Hatzack – China visit 2005 27

60 Coefficient of variation = 9.2%

FU- Frank Hatzack – China visit 2005 30

conditioning time 30 sec

FU- Frank Hatzack – China visit 2005 32

100 30 secs conditioning

Initial pellets 1 month @ R.T. 1 month @ 35 deg. C 3 months @ R.T.

75C 75C 85C 85C 90C P CT P 5000 CT Competitor 1

FU- Frank Hatzack – China visit 2005 34

FU- Frank Hatzack – China visit 2005 35

FU- Frank Hatzack – China visit 2005 36

FU- Frank Hatzack – China visit 2005 37

FU- Frank Hatzack – China visit 2005 38

FU- Frank Hatzack – China visit 2005 39

FU- Frank Hatzack – China visit 2005 40

pig trial (1008644)