XS Series E 04 Adjustment
XS Series E 04 Adjustment
XS Series E 04 Adjustment
(4) Adjust LD unit X-direction roughly so that laser beam can position at the center of the Flow Cell
image behind the FSC Detector location (now unit removed).
(4.1.13 Adjusting Laser Diode Position)
(5) Install FSC Detector at the specified position from the Flow Cell.
(4.1.14 Installing Photo Detector No.30)
(6) Adjust X and Y direction of the LD unit so that laser beam can be positioned at the center of the
beam stopper on the FSC Detector.
(4.1.15 Adjusting Beam Stopper Position)
[AA] 316E003
Cautions)
In case LD unit or Flow Cell angle adjustment is not well done, uniform signal will be seen for a
short time only (unstable). If it is difficult to get SFL signal, check if Flow Cell image at the slit of the
SSC Detector is clear and sharp when shining a flash light toward flow cell. If NOT, adjust SFL
Lens X-direction to get sharp and clear image on the slit.
(10) Verify the results in Fine mode menu, and then fix the units. (4.1.20 Optical Alignment (fine))
(11) Adjust the SSC Detector left or right so that Flow Cell image can be aligned at the slit of SSC
Detector. (Step (9) in 4.1.20 Optical Alignment (fine))
(12) Adjust X-direction of the FSC Detector so that FSC signal becomes the lowest. (This means direct
laser beam is interrupted at the beam stopper.) And then, fix the FSC Detector.
(Step (14) in 4.1.20 Optical Alignment (fine))
Caution)
Adjustment for FSC(W) data is not required as long as FSC(W) data is within the specified limit.
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(2) Connect a syringe and push CELLPACK in the flow cell to waste chamber.
(2) Lift and move FCM Detector Block toward you by loosening fixing screws.
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(10) Adjust VR1 so that the laser power is 3.4 +/- 0.1 mW with a ceramic screw driver. (A ceramic screw
driver is recommended to prevent occurring static accident.)
(3) Place a hex wrench between flow cell and laser diode, and adjust the laser diode angle so that the
reflected light and laser light are one line.
(3) Tighten fixing screws so that the flow cell angle doe not move.
(4) Confirm that the laser beam and reflected beam on the wrench are one line after tightening the fix-
ing screws.
(5) Click OK on the Laser Power screen to turn off the laser.
(3) When step (2) is unclear to observe the two lines of the flow cell, adjust the Laser Diode R/L posi-
tion by observing laser beam in flow cell as shown below. When laser beam hits the sample path in
the flow cell, reflected beam can be seen.
(3) Click OK on the Laser Power screen to turn off the laser.
Warning
Since undiluted latex is easy to coagulate, shake it enough and add to CELLPACK.
Use the latex materials within 3 hours after making it. Do not add latex to made latex
materials after a time. (Use the latex materials within an hour after making for confir-
mation of Optical Alignment (fine))
Since the fluorescence intensity becomes weak when PS-FLUO RED-2.5 (latex for
fluorescence) receives light, store PS-FLUO RED-2.5 in dark place. [AA]
Use diluted latex in a bottle.Store latex in room temperature and do not freeze it.
(9) Adjust FSC(W) approximately 0.3 or lower. If the value does not become 0.3, repeat steps (6) to
(8).
(10) Slightly loosen the screws circled in red (4 locations) shown in the diagram on the next page.
(11) Pour 1 mL of SFL latex to reaction chamber and press start switch.
(12) Adjust Photo Detector position so that the SFL wave on the oscilloscope is highest by turning
adjustment screw D.
(13) Adjust Photo Detector Position so that the SFL wave on the oscilloscope is highest and has mini-
mum variation by turning adjustment screw E and F.
(14) If the SFL wave has variation, repeat steps (12) and (13).
(15) Adjust the Photo Diode Position so that the SFL(W) is approximately 0.3 or lower by turning adjust-
ment screw D, E and F.
(16) Click Close in the screen.
313C063-R
X W
FSC 60 ± 30 0.35
SFL 130 ± 50 0.30
SSC 50 ± 40 0.35
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(2) Adjust Sample Set Unit Cover position so that the cover and chassis are flat by clicking Back or
Front.
(4) When movement completes, click Start in the Tube Rotation. Adjust the Sample Set Unit position
so that the test tube rotates by clicking Back and Front key. Adjust the Sample Set Unit position so
that the test tube rotates discontinuously by clicking Front key.
Move Sample Set Unit backward – 15 pulses by click in Back key.
Confirm that the test tube rotates continuously. Click Save for saving the position. Click Stop in the
Tube Rotation and Close key.
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(6) Tighten left fixing screw of the BR Unit No. 1. Click Close to close the window. Tighten right fixing
screw of the BR Unit No.1
(7) Set two racks to the sampler, select Controller Service Hand Adjustment and click Set Posi-
tion and Down.
(8) Adjust Hand height position so that the gap between rack and hand is 1 to 2 mm by adjusting rod
height position.
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(10) After completing adjustment, click Save Up and remove the test tube from the Hand.
(11) Click Rack-01 Catch. Place a test tube in the hand and click Down. Adjust the test tube position
so that the test tube is center of the Rack-01 by clicking Back, Front, Left or Right.
(12) After completing adjustment, click Save Up and remove the test tube from the Hand.
(13) Click Rack-02 Catch. Place a test tube in the hand and click Down. Adjust the test tube position
so that the test tube is center of the Rack-01 by clicking Back, Front, Left or Right.
(14) After completing adjustment, click Save Up and remove the test tube from the Hand.
(1) Confirm that 30 minutes past after turning on the main unit.
(2) Select Menu Controller Service Sensitivity.
(3) Enter appropriate values into HGB blank and ASP. Sensor blank, and click Send. Repeat this step
until value comes within Adjustment Value range as shown in table below.
Click Send
(HGB2)
Enter Values
(HGB1)
Confirm values are in the table
(4) Click Close after values come within Adjustment Value range as shown in table above.
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(1) Select Menu Controller Service Mode. Select Control 3 in Analytical Mode and Off in Con-
tinuous, and click OK.
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(5) Confirm the value is HGB assay (1) Enter HGB assay value (2) Click “>>>” (3) Click Send
value for e-CHECK Level 2 X 105 +/- 50 for e-CHECK Level 2 X 105
(7) Click Close when value becomes HGB assay value for e-CHECK Level 2 X 105 +/- 50.
Click Send
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Target range
DIFF-X: assay value +/- 3ch
DIFF-Y: assay value +/- 2ch
FSC-X: assay value +/- 2ch
MCV: assay value +/- 1 fL
MPV: assay value +/- 1fL
HGB: assay value +/- 0.2 g/dL
Make sure that the DIFF scattergram and FSC histogram are discrete and displayed normally.
(8) Click Close when the values are within the range.
(9) Repeat step (6) and (7) until the values are within the range.
For adjusting DIFF-Y using human blood, aspirate ten normal blood samples and Calculate mean
value. Enter target value in DIFF-Y and click ‘Send’ so that the result value will be in the following tar-
get value range.
Target Value: 43.5 +/- 3.0
- Incorrect colors
- No bright dots in the circled area - Bright dots in the circled area
GOOD example
- Expanded
- Incorrect colors