STUDY ASCP

Soluable Antigen + Specific Antibody= what reaction - Lymphokine - Soluable mediator produced by
Precipitation lymphocytes

Bone marrow stain - Romansky and Prussian Blue Increased protein in urine - renal disease

Rise in titers - Significant in serological testing Bethseda Assay - Used to quantify coagulation inhibitor

Secondary Response - IgG Platelet neutralization - Used to see if a patient has a

lupus anticoagulant.
Most common coag inhibitor - Lupus inhibitor
ITP - Ages 2-4
3' end - OH
Spherocytes - Membrane defect, Increased destruction
Haptens are only antigenic when coupled with - of RBCs, caused by intracorpuscular abnormality
Proteins
Ependymal clump -
5' end - Phosphate
MCH range - 27-33
RA - IgE
MCHC - 33-36%
Most pathogens enter through - Mucous membranes
Manual Platelet count Microscope - Phase contrase
Increased Megakaryocytes in bone marrow decreased
platelets - Folic Acid Def A.A decreased in patients with sickle cell - L-glutamine

Autoimmune disease mechanism - Circulating immune Chediak Higashi Syndrome - Albino, Photophobia,
complexes pyonic infections, giant dark granules in neutrophils and
monocytes
Cells Positive acid phosphatase, not inhibited with
tartate - Hairy Cell Leukemia Aspirin - Not likely to cause an abnormal thrombin time

Most common SLE - nuclear antigen RBCs are usually smaller - than the nucleus of a
lymphocyte
Light Chains - Kappa/Lamda
-a/aa - silent carrier
Mononucleosis - False positive RPR
CD20 - B cell marker
Negative Glucose oxidase and positive reducing sugars -
Non-glucose reducing sugars such as galactose Platelets - do not circulate in inactive form, Spiny sticky
form become activated in response to blood vessel
Hep B vaccine - Passive Immunity damage

Titer - Recipricol of maximum reactive dilution Hemoglobin Chromosome 16 - Alpha zeta

Decreased complement - In lupus Hemoglobin Chromosome 11 - beta, delta, episilon and

gamma
Clinical signs of HIV but AB test are negative - Window
phase before AB production Megaloblastic anemia - Oval microcytes and
Hypersegmented neutrophils
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Abnormal Granulation - Alder anomaly & Chediak-
Doesn't soley develop in the bone marrow - Higashi
Lymphocytes
Hemoglobinopathies can not be caused by - The
Transferrin Saturation - (serum Iron/TIBC)X100 deletion of a global chain

Beta Thalasemia minor - B+/B, B0/B Hemolytic anemia - Not where basophilic stippling is
found
Howell-Jolly bodies are composed of - DNA
Alpha Thalassemia - Decrease in the rate of production
CLL CD - CD19, CD20, CD23, co-expression of CD5 of Alpha chains due to partial or total deletion of the
genetic code
ADAMTS13 enzyme - Cleaving ultalarge molecules of
von willebrand factor to keep them out of circulation Which is NOT associated with Macrocytosis -
Thalassemia
Least likely to stimulate production of reactive
lymphocytes - HIV Warfarin inhibits vitamin K dependent factors - 2,7,9,10

Why ferritin is less than optimal for HH - It is an acute Flow cytometry is NOT a useful tool in - Hepatitis
phase reactant that is elevated in a variety of clinical
conditions Clot would have most of an effect on - Platelets

Barr Bodies - Normal in Females not in males B0/B+ - Intermedia/Major

Hexose monophosphate shunt - Provide Reduced B thal major - Cooleys anemia

glutathione to prevent oxidation of hemoglobin
% of US individuals heterozygous for HFE - 10-12%
CML Vs Leukmoid Reaction - LAP stain
HBS - Glutamic Acid substituted for Valine
Aquired Hemophilia A - Anti-Factor VIII inhibitor
Peroxidase stain is useful in differentiating -
HbS Protection against Sickling - HbF Lymphocytic and granulocytic cells

Absolute Cell count - Total count X % of cells counted HgH are tetramers of which globin chain - Beta

Early abnormality in lymph node of AIDS patient - LAP stain used to determine - Leukmoid reaction from
Reactive Lymphadenopathy CML

Clinical symptom thrombocytopenia - Petechiae Alpha hemoglobin chain coding - 4 gene loci Each on
chromosome 16
Lymphocyte - Pale-Blue Cytoplasm, Nucleus oval or
round Myeloblast -

Monocyte - Blue-Gray opaque cytoplasm, Nucleus Indwelling catheter contaminated by heparin -

convolutions Prolonged PTT and normal reptilase time

Newborns will develop 30% of what HB in HbH - Bart Not associated with Hereditary Spherocytosis -
Intravascular Hemolysis

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Most side scatter - Granulocytes
Hemocytometer - generally not used with synovial
Lupus Inhibitor confirmation - Russell Viper Venom crystal counts

C3 - found in both classical and alternative pathways Primary hemostasis resulting from vascular damage
includes all of the following except - Vasodilation
Fc region of Antibody - Responsible for activating
complement Increases HbS Polymerization - Acid pH, Increase 2'3
BPG
Bone Marrow Collection - Posterior iliac crest
Monospot - Horse RBCs
Avidity - Overall Strength, Multivalent Antibody
Pappenheimer bodies - Splenectomy
Not considered necessary for making a diagnosis of
Hereditary Hemochromtosis - Family history Macrophage cytokine - Interferon Gamma

Which hemoglobin is known to migrate with HbS during Purpose of full pre-transfusion crossmatch - Detect AB
electrophoresis when an alkaline gel like cellulose is in recipient serum plasma that react with donor red
used - D cells

Barr Bodies are classified as - non-pathological Essential Thrombocythemia - Increased in number of

platelets and functionally abnormal
RT-PCR - Measures at Exponential Stage, All cycles
including detection can be completed in the same time End point initial iron depletion phase HH - Serum
ferritin decreases 20-50mg
Standard PCR - Measures PCR at Plateau stage,
Followed by electrophoresis Granulocyte concentration storage - 20-24 degrees
celcius for <24 hours
Characteristic of a Patient with Von Willebrand disease -
Abnormal Platelet aggregation with response to Yellow color found in CSF - Xanthochromia
ristocetin
Sicke cells - return to normal shape when deoxygenated
May increase ESR except - blood drawn into a sodium
citrate anticoagulant Normal platelet count - VWD

Adequacy of fibrinogen in heparinized patients - CML - Low LAP Score (11)

Reptilase time
CMV seronegative RBC use for exchange transfusion -
Immune Positive TB test - T cells and Macrophages Leukoreduced RBC

Waldenstrom's Macroglobulinemia Increased - IgM Irradiation is used for - Graft Vs Host disease

Contact dermatitis is mediated by - T lymphocytes DAT is indicated in all of the following EXCEPT -
Detection of alloantibodies in serum
Heinz Bodies - Supravital stain
Group A - N-acetylgalactosamine
Prozone - High Titer of Antibody
Blood Transfusion samples - 7 days
A Thalaseemia slight decrease in MCV MCHC - Minor
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Not expected with HH - Elevated hemoglobin and HCT
Bicarb - Most carbon dioxide in the blood is in the form
Reconstituted Deglycerolized RBCs good for - 14 days
Parvovirus B19 - Causes infection in patient with sickle
Directed blood donations - are perceived safer but not cell disease
safer
Cells are gated by - placing a gate around cell
All T lymphocytes are positive for - CD3 populations with the same light scatter

Antibodies Enhanced agglutination with proteolytic Glucuronic Acid - is conjugated with indirect bilirubin to
enzymes - anti-Jka,Jkb,C, E form direct bilirubin

Reticulocyte count is used to assess - RBC production by Fibrinogen - would be seen if plasma rather than serum
bone marrow was subjected to electrophoresis

Myleodysplastic syndromes - Qualitative disorder of FSH - is produced by the pituitary

erythroid, myeloid and megakaryoid
All of the following are characteristic of the role of CRP
Lipemia effects - MCHC EXCEPT - Binds to factor H to initiate the alternate
complement pathway
Normal M:E ratio in bone marrow - 2:1-4:1
Polyacrylamide gels - have the greatest resolution or
Absence of what clotting factor causes no bleeding separation of solutes
tendancy - Hageman
Increased AFP is associated with - Hepatocellular
B cells - CD19/CD20 carcinoma

Closely related to ABO antigens on the red cell Estrogen and Progesterone - Are used for Breast cancer
membrane - I,i
CA125 - Ovarian Cancer
Blood donation interval - 56 days (8 weeks)
Serum Barbituate Confirmation - Gas-Liquid
Bite Cells - G6PD deficiency chromatography, Mass Spec

Rh immune globulin therapy provides - Passive 17-ketosteroids - Testes and adrenal cortex
protection
Platelets not take from apheresis - must be processed
A2 cells - have more H antigen than A1 within 8 hours

Red cell survival is measured by - Cr51 Distinguish between bacterial infection and CGL - LAP

If transfused patient can not donate for - 1 year Source of irradiation of blood products - Cs137

Drabkin solution - Cyanmethhemoglobin Dietary iron and iron absorption - Only a small
percentage of diet iron is absorbed and used
LeA antibodes are - IgM
Follow up testing for speckled ANA pattern - ENA
8 Hydroxyquinoline and orthodo-cresolphthalein
complex (CPC) - Calcium DNA Isolation - Salts
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Anti-HBc IgM - Acute Infection All of the following are true regarding CK-MB (CK-2)
except - Ck-MB levels are normal during cardiac
Which of the following blood types would not react ischemia
with Anti-Fy3 - Fy(a-b-)
Most common reaction with bilirubin are based on the
Hydroxyurea - Induces increased production of HbF reaction of bilirubin with - Diazo Reagent

Alpha thalassemia major - incompatible with life Drugs take - 5-7 half lifes to reach steady state

Ketone reaction measured by nitroprusside - Chylomicrons are primarily composed of - Triglycerides

Acetoacetic Acid
Cushing - High serum cortisol, moon face
Wash Cord blood - To remove whartons jelly
Characteristic of someone with beta thalassemia
Marked rise in ALP, conjugated bilirubin and slight rise intermedia - Some splenomegaly
in ALT - Post hepatic cholestasis
Bioavailability - Fraction of drug that is absorbed into
Prozone described by all the following EXCEPT - Results systemic circulation
in a false positive reaction
BHU accumulation of - Acetyl CoA
Doppler sonography is - preferred choice of monitoring
HDFN Common characteristic of hereditary spherocytosis,
eliptocytosis, stomatocytosis, Paroxysmal nocturnal
Acetest can be used for ketones in - Whole blood, hemoglobinuria - Red membrane defects
Urine, Serum
Which Adipocyte products is an important messenger in
CK-MB - Used to detect Reinfarction metabolism signaling the hypothalamus that there are
changes in fat stores - Leptin
X-linked agammaglobinulemia - Flat gamma SPEP
If CSF tubes can not be analyzed within 1 hour the
Type 1 diabetes increased - Ketone bodies and blood microbiology #3 tube should be - Left at room
glucose temperature

L/S - Respiratory distress syndrome Elevation in conjugated bilirubin is most likely found in -
Biliary obstruction
Most likely to interact with arterial walls, leading to
deposition of cholesterol and initiating or worsening Primary Hypothyroidism, Hashimotos thyroiditis -
artherosclorsis - Small dense LDL Decreased T4, Increased TSH, Increased TRH stimulation

TIBC is a direct measurement of - Transferrin Increased blood ammonia levels - End stage cirrhosis

Falsely elevated on non-fasting specimen - Triglycerides VLDL - Endogenous Triglycerides

CRP - Low specificity Hormonal variation during the menstrual cycle typically
shows - An abrupt rise in LH 3-36 hours prior to
Increase LDL - Heart Disease ovulation

Decreased Haptoglobin - Intravascular hemolysis

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Isoenzymes of alkaline phosphatase - Kidney, bone, Cause of metabolic alkalosis - Excessive vomiting
intestines and liver
Neutral 17-ketosteroids - Androgen production
Angiotension 2 - Increased vasoconstriction
Ovarian cancer - CA125
Primary Hyperparathyroidism - Elevated calcium,
Decreased Phosphorus Acute Phase Proteins - Glycoproteins

False statements about lead - Serum lead level is a good pH - glass electrode
inexpensive screening test for lead toxicity
Which test has been recommended by the FDA to
Deficient secretion of bilirubin into the bile canaliculi - replace the HIV-1 p24 antigen test in screening of blood
Dublin-Johnson syndrome for infectious diseases - HIV-1-NAT

Risk marker with both negative and positive Homozygous cells are used because - Stronger reactions
cardiovascular risk integrated into its measurement - are seen with homozygous cells
Apo B-100/Apo A-1
How many IU are in 300ug of RHig - 1500
True statement concerning Alkaline Phosphatase -
Increased in obstructive jaundice Passive Anti-D Less than or equal to - 2+

Electrophoretic separation of LD that shows LD-1 Explanation for negative Anti-A, Anti-B, A1, B cells on
greater than LD-2 could be indicative of - Hemolysis baby - The babys antibodies are underdeveloped

TSH - decreased in hyperthyroidism Most common type of autoimmune hemolytic anemia -

Warm autoimmune hemolytic anemia
Elevated in breast cancer - CA 15-3 CA-549
Hemoglobinemia and hemoglobinuria - will not be
Contributes to specific gravity but not osmolarity - present in extravascular hemolysis
Protein
Autologous blood must be tested for which of the
Why small dense LDL molecules are artherogenic - have following before transfusion - ABO, RH
greater mobility in the endothelium and arterial wall
Proteolytic enzymes are useful in - Kidd system
Which of the following non-HbS hemoglobins can also
sickle and show a positive solubility test - HbC harlem A1 subgroup represents - 75-80% of type A

INCREASED serum Phosphorus/Decreased Calcium - Unexpected positive reactions encountered during

Hyperparathyroidism forward ABO typing may be due to - Acquired B antigen
due to intestinal cancer
Gel electrophoresis with HbS - HbS migrates with HbD in
alkaline and alone in acid electrophoresis Antibodies enhanced by enzymes - Rh, Lewis, Kidd

Serum ALP activity is derived from all of the following Most common antibody in HDFN - Anti-D
organs except - Brain
Kle B - Is used to quantitate FMH but has poor
Myocardial infarction which of the following enzymes reproducibility
will be the first to become elevated - CK
Most common Rh0 D negative genotype - rr
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RH,Lewis, Kidd - Enhanced by enzymes What should be done first if a donor unit is
incompatible at the antiglobulin phase with several
All of the following are benefits of autologous donation different recipients - Perform a DAT on the donor unit
EXCEPT - Are on hand in case of an unexpected
emergency How many vials of Rhig need to be added after dosing -
1
What can be shipped with FFP - frozen RBCs and cryo
Antibodies to what cause febrile transfusion reactions -
Most commonly associated with febrile non-hemolytic Granulocytes
transfusion reactions - Immune response to leukocytes
RBCs during shipping - 1-10
Lookback - Procedure by transfusion service when
notification is received that a donor unit service now RF - IgM
test positive for an infectious disease
Patient presents with strep throat beta hemolytic
RBCs split using open system, must be used within - 24 catalase neg - Arcanobacterium haemolyticum
hours
Mild allergic reactions during transfusions whats the
Which is not a major Rh antigen - d cause - Plasma proteins

HDFN due to Anti-D would be rarest in - Asians Antimitochondrial abs - Biliary cirrhosis

A2B patients have - Serum containing Anti-A1 and Red Rheumatic fever - anti myocardial abs
cells that react with Anti-A and Anti-B
Spinal fluid newborn, small grayish translucent beta
Autologous donation time frame between donation of hemolytic, small gram positive rods like dips - Listeria
surgery is - 72 hours monocytogenes

Shelf life of CPDA-1 is - 35 days Lower reference limit for progressive motility of sperm -
32%
Mycoplasma Pneumoniae - Anti-I
TSH High, Low T4, T3 - Thyroid established as cause of
If forward and reverse ABO are negative - Incubate at hypothyroidism
room temperature or 4C to enhance weak expression
Chain responsible for differences among
Routine titration for pregnant female given rhogam and immunoglobulin chains is - Heavy Chains
has Anti-Dat delivery - F (not considered good practice)
Following LEAST useful in differentiating hemophilia A
Hemolysis in post transfusion blood sample indicates - from hemophilia B - aPTT
ABO incompatibility
Not associated with delayed hemolytic transfusion
Why do few patients transfused with uncrossmatched reaction - Unexplained Bleeding from surgical site
red cells experience hemolytic transfusion reaction -
The incidence of red blood cell antibodies is low Bence jones is seen in all of the following except -
Nephrotic syndrome
Most definite indication a patient has been sensitized to
an antigen - Positive post transfusion DAT, with All of the following would be associated an increased
previously negative DAT level of AFP except - Prostate cancer
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Salts - Pull DNA from the sample Staph epidermiditis - not associated with culture
negative endocarditis
thin-walled, two or 3 celled macroconidia, no
microconidia - Epidermophyton alpha thalasemmia minor - -a/-a

Nephelometry Vs Turbidity - Nephelometry directly Antigens closely related to ABO antigens on red cell
measures the amount of light scatter by particles in membrane - I,i
solution, and turbidity measures the decrease in
incident light intensity Genotype screening in pharmacogenomics - genotyping
does not take into account drug interactions which can
KB test - difference between maternal and fetal red affect metabolism
cells
Antigens well developed on fetal cells - Kell
Lower threshold for pH of semen in males - 7.2
Automated chloride level reading - Amperiometric-
Jendrassik-Grof unconjugated bill with diazo reagent - coulometric titration
Caffeine benzoate
Sim tube, listeria monocytogenes, umbrella motility - 25
Benefit most from washed red cells - IgA def with anti- degrees
IgA
Protein S - not an acute phase protein
Marked elevation 5-HIAA - Carcinoid tumor
Positive Bile solubility test - S. pneumoniae
Genes that initiate synthesis of DNA, promote cell
division and inhibit cell death - porto-oncogenes Least likely associated with thrombotic tendency - APTT

Cause a donor to be deferred - diastolic pressure of Hemo A, B, VWD make up - 90% of hereditary diseases
110mmHg
False troponin - Rises sooner after MI than CK-MB
Enterobacteriaceae that produces Dihydrogen sulfide -
Salmonella Pluripotent stem cells give rise to - Lymphoid and
myeloid stem cells
Platelets surrounding neutrophils on differential and
low platelet count - Recollect not using EDTA apolipoproteins - are essential for lipid metabolism

Exudate - Leukocyte count greater than 1000WBC/ul Good test for screening HH - Transferrin Saturation

B and T lymphs are derived from - Hematopoietic stem Diluted reagent would result in what kind of error? -
cells Systematic

Histoplasmosis - Dohle bodies - Sepsis

Platelet storage - 6.2 Patient suffering from Celiac disease and Crohns disease
is given a new oral medication that has a narrow
CD5 - Mature T Cells therapeutic window. Which is true? - TDM would be
helpful to see how much has been absorbed
Red cells positive DAT, Removal of AB from cells - AOA,
Ficin, ZZAP, Chloroquine diphosphate
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Which of the following is not true about hybridization in
PCR procedures - Hybridization cannot happen between Two medias appropriate for bordatella pertussis -
a strand of DNA and a strand of RNA Bordet-Gengou, Regan-Lowe charcoal agar

Best way to thaw FFP - is placing it in a 37 degree or Infectious mononucleosis - Reactive Lymphocytes
lower bath
Storage temperature of enzymes - 4 C
Sheep blood agar contain an inhibitor to which of the
following organisms - Haemophilus HIPAA - Health INSURANCE portability and
accountability act
Semen specimen collected 3 hours before testing -
Report as compromised on the final report Greatest risk for maternal rubella during gestation - first
trimester
Not an application of PCR - Amplification of DNA for
northern blot Valinomycin - Potassium

RAST - most helpful in identifying specific allergens Helminthosporium -

Xanthochromia in a premature infant may be due to - Campy fetus jejuni - Optimal growth at 42
AOA-immature blood brain barrier, Elevated protein in
CSF, Elevated bilirubin in blood Hymenolepis diminuta -

Candida parapsilosis - Shapes used to describe cocci - Ovoid, lancet, round

Kidney graft best chances - HLA identical, mixed Methylene Blue Stain works by - Staining cellular
lymphocyte culture (-), ABO identical components

Acute phase proteins are synthesized in the - Blastomyces dermatitidis -

hepatocytes
Trichoderma -
Blood lactate concentration will - increase if circulation
and oxygenation are impaired Frozen RBC cells in glycerol are frozen at - -65 or lower

17 ketosteroids - Testes and adrenal cortex ABO groups discovered by - Landsteiner

Few small purple inclusions are found in erythrocytes, Group II discrepancies - forward and reverse groupings
they can be confirmed as containing iron by - due to weakly reacting or missing antigens
performing a Prussian blue stain
Cryptospora - 5-8 <cyclospora
Secondary hemostasis - interrelated chemical processes
that lead to the formation of durable fibrin strands 15% of population - is RH neg

Moraxella catarrhalis - URI, sputum, gram-negative Enzymes - Proteins of biological origin

diplococci, Oxidase Positive, carb degradation test
inactive A-B-O- cells - only RH and ABO must be done

300 dose of rhogam - 30 ml WB Myocyte contraction regulator - cTnT

Nanometer - 10^-9 Precision - Method Reliability

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Calcitonin synthesized - Thyroid C cells
Lower limit for semen ml - 1.5ml
Hyperosmolar non-ketonic coma - 70 year old type 2
In PCR what is amplified - Target diabetic patient

SDI - position of your test compared to the mean of the Least affected by fasting - Cholesterol
test group
Decreased GFR - Creatinine
Whole blood glucose levels - are 11% lower than plasma
Argue in favor of TDM - drug is given chronically
In a closed system when FFP is thawed it can be labeled
thawed plasma 24 hours after the original thaw time AFP - used to determine neural defects
and given a shelf life of - 5 days
CSF Tubes - Chem, Micro, Hemo
Jaundice - is associated with low levels of glucuronyl
transferase Organic iodine - Thyroxine

Serum ferritin level suggestive of iron overload in a pre- iontophoresis - induces sweat production
menopausal woman - 200mg
Thin-layer chromatography is particularly useful in
When preparing red cells for elution - Thoroughly was identification of - Drugs
cells
5-15% of - Iron is absorbed daily
Thalassemia shows no anemia and may not be detected
using hemoglobin electrophoresis - Beta thalassemia CEA - Colon
minima
IF not Folate - is needed for the ABSORPTION of B12
Which component is most commonly used to replace
multiple coagulation factor defs in bleeding patients - Jaffe reaction for creatine - Saturated picric acid and
FFP sodium hydroxide

Epstein barr syndrome - >20% atypical reactive Multiple myeloma -

lymphocytes
Shows a decrease during an acute phase response -
LDL - Transports majority of cholesterol to cells for Albumin
steroid hormone synthesis
Skeletal muscle enzyme - Aldolase
End product of epinephrine and norepinephrine - VMA
Metabolic syndrome - Fasting blood glucose, trigs, HDL
Inhibits pituitary secretion of ACTH - Cortisol
Fructosamine not HA1C - long term for sickle cell
Cellulose acetate electrophoresis separates into - 5 anemia
fractions
Ion selective electrodes are called selective because
Good candidate for TDM - drug with a narrow they measure - Activity of one ion much more than
therapeutic window other ions present

HA1C 6% - Patient is compliant with diet and medication Mg activates - Alk Phosphatase, CK

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Troponin I - 3-12 hours for 7-14 days Red blood cell formation of large amounts of
hemoglobin usually begins with which developmental
Major property of ADH - Changes distal tubule water stage - Polychromatic normoblast
permeability
Main site for monocytes after diapedesis - Body tissues
After second spin in the preparation of platelets they
should - Allowed to rest for 1-2 hours Basophil - Immediate hypersensitivity reactions

DR antigens - HLA system RDW in alpha thalassemia - Increased

Platelet donation minimum - 5.5x10 Down syndrome - ALL

Components provide best source of fibrinogen with G6PD - Precipitation of Hb

patient with fibrinogenemia - Cryoprecipitate
Dry tap - Touch Preperations
Fya - destroyed by enzymes
Direct cause of HH - Absorption of excessive amounts of
Common difference between PCH and CHD - PCH iron in the small intestines
caused by IgG CHD is caused by IgM
False positive ketones - L-dopa metabolites
Type B blood - has higher incidence in Asians
Bile duct obstruction - Positive because conjugated
Consequences of HDFN most associated before death - bilirubin is present and excreted by the kidneys
Cardiac failure
Transitional epithelial cells -
AB blood patients - still have H antigens
Watson Schwarts - Urobilinogen
FFP should be transfused within - 24 hours
Activation unit for complement - C4, C2,C3
Allogenic blood - Donated by someone OTHER than the
donor Opsonins - Greatly enhance phagocytosis in RE cells

Major crossmatch purpose - Verify donor ABO packed ANA shows speckled pattern - Screen for Sm and RNP
cell compatibility antibodies

To prevent Rh alloimmunization initiate Rhogam to - Rh Threshold limit value - Maximum level of a chemical to
negative pregnant females, anteatal in the 3rd trimester which a worker can be exposed without causing adverse
health affects
Antibody most likely negative for HDFN - Anti-A
Class C fire - Electrical
Anaphalxis - on RBCs lacking IgA
Safety regulations fall under what organization in the
Leukmoid Reaction - WBC >50,000 clinical laboratory - OSHA

The dissolution of a clot with 5M urea indicates which Probe - Nucleic acid used to identify a hybridization
of the following factor defiencies - XIII target

Preparation of standards - Reagent grade

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HLA-A and HLA-B can be detected by - Lymphocyte Specific gravity - Relationship between ionic
cytotoxicity concentration and specific gravity

A standard is used to check the - Accuracy Which hormone does not stimulate gluconeogenesis
(formation of glucose from non carbs) - Insulin
Lymph nodes of aids patients - Reactive
lymphadenopathy Significant titer - 8-32

Diapedesis - How long neutrophils reside in peripheral All of the following associated with hemoglobin C
blood 7-8 hours except - fast motility of HbC at pH 8.6

Cordocentesis - Procedure used to obtain fetal blood Blood gas anticoagulant - heparin
sample to monitor severity of HDFN and can be used to
deliver intrauterine transfusions High Specificity - Accurately identifies the absence of
disease
Which antigen might show a cell typing change during
pregnancy - Lewis Semen 3 hours after collection - Report as compromised
in final report
Russell viper venom test - Confirmatory test for lupus
anticoagulant Sudan III - Presence of Fat

10-12% - of people in the USA are heterozygous for HFE Why serum ferritin is not optimal for screening test of
mutation HH - Acute phase reactant that is frequently elevated in
a variety of clinical conditions
Semi-automated and automated urine chemical reagent
strip readers - Remove subjectivity allowing for better Routine sperm count dilution - 1:20
reproducibility
Glomerular filtration mechanism - hydrostatic
When evaluating the throughput of a particular method differential in glomerular tufts
you should consider all of the following except -
Sensitivity of technique Chediak Higashi - Abnormal granulation

Right to know law - Hazard communication 1910.1200 Joint fluid does not - have the same consistency as
plasma
Storing reagents without expiration date - Store in a
manner where the oldest will be used first Coombs reagent contains - Mono/Polyclonal antibodies,
anti-C3b
Corneal tissue transplant - absence of class II
transplantion antigens Monitor Precision - Run samples in duplicate

Outpatient reimbursement code - Common procedure Used to separate DNA >50 - Pulsed field electrophoresis
terminology codes
CSF WBC 60-100% - Lymphocytes
Half-life IgG - 23 days
Newborns who develop hemoglobin H may
Relative centrifugal force of a centrifuge may be demonstrate up to 30% of which type of hemoglobin -
affected by all of the following except - Time Bart

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Hgb that protects against sickling in patients with HbS - Estriol, HcG, Inhibin, alpha-fetal protein can be obtained
Hgb F during pregnancy to - Assess Down syndrome

Drawing with a butterfly - Draw and discard a waste Alcohol in gram stain to - Decolorize all gram negative
light-blue top tube before the tube that will be used for organisms
coagulation studies
Primary reagents in aPTT - Activated partial
Heterozygous sickle cell - HgA/S thromboplastin and Calcium

Endpoint iron depletion phase for Hereditary Anaphalactic shock - IgE

hemochromatosis - Serum ferritin decreases to
between 20-50 largest constitute of non protein nitrogen - Urea

Screening test for hemoglobin S based on - Decreased Most common type of autoimmune hemolytic anemia -
solubility of deoxygenated Hb S in solution that WAHA
produces turbidity
BNP - synthesized and secreted in the heart
Barr bodies - non-pathological
Enzyme mutliplied immunoassay technique differ in
Basophil granules - Histamine that they - no separation of bound and free antigen is
required
NK lymphocyte - Large cell with abundant cytoplasm
and a few azurophillic granules Porphyrins - Urine dark wrapped in foil ph 7.5

>50% of lymphocytes found on peripheral blood smear Cabot rings - Megaloblastic anemia
of a 5 month child - Normal finding
No diabetes 45 and older should get screened - every 3
Drabkins solution - Cyanmethemoglobin years

Bone marrow M:E ratio - 2.5:1 2 hour glucose test on pregnant woman - <140

Pathway initiated when collagen is exposed - Intrinsic SLE - Homogenous pattern

All of the following describe T lymphocytes except - Percentage of glycerol used when freezing RBCs - 40%
Production of surface immunoglobulins
HbF 100% - Delta-Beta thalassemia major
Beta Thalassemia intermedia - Some Splenomegaly
Pasteurella - Cat bite
Bone marrow cellular morphological - detail cant be
determined Normal adult hemoglobin A - Alpha and beta

Hemo test most affected by microclots - platelet count What would rule out TB - Yellow colonies when exposed
to light
Essential thrombocythemia - Platelets are increased and
abnormal P disc resistant - Strep viridins

Minimum volume of RH pos red cells needed to Which test is normal in a patient with dysfibrinogemia -
produce Anti-D - <0.1 ml Immunologic fibrinogen level

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T lymphocytes - CD3
Reduce Zeta potential in RBCs by - PEG, albumun or
Zollinger Ellison disease - Gastrin enzymes

Syphillis microscope - Darkfield Which does not develop in the bone marrow -
Lymphocyte
Macro-Amylasemia - Increased serum amylase, Normal
to low urine amylase Pheochromacytoma - Metanephrines

HCT - % of blood made up of blood Pippette should be wiped off - BEFORE lowering the
miniscus to the calbration mark
HbD is elevated in all of the following except - alpha-
thalassemia Monoclonal B cell population CD19,20,23 and 5 - CLL

Neisseria gonococci and Neisseria meningococci can B antigen mixed field - B3

oxidase - Tetramethyl-p-phenylenediamine
dihydrocholoride Bence jones heat precipitation test - Precipitates
between 40-60 but redissolves at 100
Hep B recovery 6 month later - Anti-HbS
Proteins in 8.6 pH will become - NEGATIVELY charged
HCG - Rapid rise in levels during first trimester; slow and migrate to the anode
decline and possible level off throughout remainder of
pregnancy Unexpected positive result during ABO forward typing -
Acquired B antigen
Which is NOT true of RT-PCR - Does not require use of
reverse transcriptase Adipokine decreased in obesity, anti-inflammatory,
increases insulin sensitivity - Adiponectin
Occupational exposure greatest risk of HIV transmission
- Percutaneous injury Cenfugical force affected by everything but - Time

Specificity - % of patients without a disease who will Six sigma improvement - Failure mode and effects
have a negative test analysis

Mild allergic reaction to a transfusion is caused by - Radiation source for atomic absorption - Hollow
Drugs or food consumed by the blood donor cathode lamp

Bacteria in urine - Alkaline Best correlates to HDFN - Aminotic fluid bilirubin

Weak D - Reduced number of D antigen sites on surface Corneal tissue transplant - Absence of class II
of red cell transplantation antigens

False positive for Syphillis RPR - Mono Nasopharynx of healthy people but rarely pathogenic -
Cornyebacterium
True monocytic leukemia - M5
HbS polymeration increase -
Reagent strip primarily test for which type of protein - Acid/dehydration/INCREASE 2,3
Albumin

False negative result in AHG testing - Low pH of saline

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Important messenger in metabolism signaling Sequestration crisis sickle cell - exchange transfusion
hypothalmus that there are changes in fat stores - and or splenectomy
Leptin
Out of how 1,000,000 caucasians will have the following
Diagnosis of diptheria is determined by - Determining phenotype? Group O,K+, Jk(a+) - 30,000
culture is a toxin producing strain
Influenza A - Is worst than Influenza B or C
Adequacy of fibrinogen in heparinized patients -
Reptilase time CSF lymphocytosis is associated with all of the following
except - Cerebral absess
NOT used to classify acute leukemia - Retic Count
What requires a complex lymphoblastoid cell culture -
Seal on RBCs opened how long can it be refrigerated Epstein Barr
prior to administration - 24 hours
Urine test strip protein - Albumin
Equivalent to CMV neg - Leukoreduced RBC
10 large hemocytometer squares contain - 1ul of fluid
McLeod - Kell
Chylomicrons are composed of - Triglycerides
Pos/Neg Cardiovascular risk - Apo B-100/Apo-B-1
Warfarin inhibits what factors - Vit K dépendent
Hemoglobinapathy - Substitution of an amino acid in 2,7,9,10
the hemoglobin chain
CAD cold agglutinin disease - anti I
Non invasive way to predict anemia in fetus - Color
doppler middle cerebral artery peak systolic velocity VLDL - Endogenous

Gene loci for alpha chain are adjacent to locus for what Not a learning domain of a MLS program -
globin chain - Zeta Comprehension domain

Conditions associated with polyclonal broadbase Procedure that utilizes leukapheresis to collect Buffy
increase in gamma globulins except - Immunodeficiency coat from whole blood - Photophoresis

Virus that causes aplastic crisis in HbS - Parvovirus B19 Oliguria - Acute glomerulonephritis

Enzyme responsible for conjugation of bilirubin - M tuberculosis to aggregate - Cording factor

Glucurynol transferase
Beta thalassemia that shows no anemia and may not be
Circulating organic iodine - Thyroxine detected using hemoglobin electrophoresis - Beta
Minima
Dimorphic species that does not produce a yeast colony
when incubated at 35 - Coccioides Immitus Lower reference level for semen - 32%

Normal Spinal Fluid Volume - 90-150ml NAA techniques - Target amplification/signal

amplification
Elevated conjugated bilirubin - Biliary obstruction
Nephrotic all include of the following except -
Hydroxyuria acid - Induces increased production of HbF Hyperproteinemia

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Not a feature of SLE - Increased serum complement
Anti malarial drugs hemolytic anemia - G6PD Def
Find in DIC not TTP, HUS - Prolonged PT/PTT
Fibrinogen def - Cryo
Red blood cell transfusion infections - Yersenia Pestis
Found in both classical and alternative pathway - C3
Contributes to specific gravity but not osmolality -
Protein Best describes neutrophil - Cytoplasm of cell contains
specific granules
Not a possible cause of death after HIV infection -
Penetration of membrane by the HIV spike Does not require computer label on blood - Outdate

Minimum HgB for autogenous blood - 11g Class B - Grease fire/Flammable chemicals

Best likelyhood to receive D positive RBCs without Distal Convoluted tubles - Maintain water-electrolyte
adverse effects - C in trans to RHD and acid base balance

Competitive Radioimmunoassay - Antibody will react RBC that does not react with Anti-Fy3 - Fy(a-b-)
equally with labeled and unlabeled antigen
False regarding troponin - Rises sooner than CK-MB
Malaria blood deferrment - 3 years
Benefit from washed cells - IgA def with anti IgA
Absence of what factor causes no bleeding tendency -
Hageman VLDL - Endogenous

A1 subgroup makes up - 75-80% Increased alpha thalassemia EXCEPT - Iron binding

capacity
Wilsons disease - Increased urine copper
Rh antibodies - Immune IgG
Most common nucleated cell in bone marrow -
MetaMyleocyte RDW in alpha thalassemia histogram - Shifted to the left

Not a koch postulate - When organism is injected into Alpha thalassemia intermedia crisis - Increased LD and
HUMAN will produce disease Bilirubin and normal haptoglobin

DNA virus - Hep B F serum lead - Serum lead level is an good inexpensive
screening test for lead toxicity
Abnormal assay associated with thrombotic tendency -
APTT Basophilic stippling - Aggregates of ribosomes

Confirm pence jones - Urine protein Elect, Myocyte contraction - cTnT

immunoelectro
Calcitonin increase - Medullary carcinoma of the thyroid
CSF Multiple Scleorsis - Lymphocytes
Shows presence of disease - Sensitivity
Ammonia - Waste product of AA and protein
metabolism Granulocyte concentrates be administered after
collection - 24 hour
False about urea - Decreased in Dehydration
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Lymphocytes - GFHD
Source of HIV envelope - The host cell membrane
First enzyme to be elevated during MI - CK
Which antibody causes HDFN but limited to less severe
Capillary electrophoresis - occurs in a capillary tube with symptoms - Anti-A,B
detection while the solutes separate and migrate in the
tube HgH - Directly alters the integrity of the RBC membrane

CSF for syphillis - VDRL Community associated MRSA - is predominantly

associated with skin and soft tissue infections
Falsey elevated hemoglobin - Lipidemia icterus
HIV - RNA is used as a template for DNA synthesis
Neubauer hemocytometer - 9 squares
ALP - increased in obstructive jaundice
Group II discrepancies - antigens
Autoantibody found in all sera - Anti-I
Viral and rickettsia testing - IFA
Proper temperature when cross matching in presence
Percentage of bleach - 10% of cold antibody - 37

Hyperparathyroidism - I(CA) D(Ph) Must be labeled with a biohazard label or be colored

red or orange to indicate biohazard - A container that is
HLA - Allergic tranfusion reactions used for disposal of needles in the blood collection area

EDTA and Sodium citrate combine with - CA to inhibit Potassium ferricyanide - Ferrous iron is oxidized to ferric
coagulation iron to form methehemoglobin

Continuous occurance on one side - Shift Platelet concentration technique - Light spin followed
by a hard spin
Specificity - True Negatives
After second spin in preparation of platelets - They
Sensitivity - T positives should be allowed to rest for 1-2 hours

Fluorometer design - Prevent excitation light from Electric fire - Class C

reaching the detector
Major spill of a carcinogen has occurred - Do not
NRBCs most likely seen on - Beta Thalassemia Major attempt to clean it up; follow your laboratorys
hazardous materials emergency prodcedures
Acute phase proteins - Glycoproteins
Levy jennings QC requires - a minimum of 20-30
Strep G - Deep wound cellulitis analyses of control serum over a period of 20-30 days

False positive bilirubin - Large doses of Standard precautions - All specimens are handled as if
chloropromazazine they are hazardous and infectious

Most often associated with transfusion reaction of Paper wood or cloth fire - Class A
person lacking IgA - Anaphalaxis
Right to know OSHA law - Hazard communication
Molecular method not used - Donor antibody screening
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Relative centrifucal force is function of - Radius and
RPMs

QC run +2SD and -2D out of QC - Poor precison resulting

from random error

95% confidence - +/- 2SD

Analytical sensitivity - ability to measure low

concentrations of analytes

All of the following statement about fluorometry are

true except - Fluoresence is indirectly proportional to
the analyte being measured

Spectrophotometric absorbance is related to %

transmittance in what way - Inversely and
logarithmically

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