The UK Crop Microbiome CryoBank

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THE UK CROP MICROBIOME

CRYOBANK

Locations United Kingdom

Dates 01/10/2020 - 30/09/2025

Summary
Plant microbiomes are the microbial communities essential to the whole
ecological area of a plant’s ‘phytobiome’ – a term used to describe a plant’s
specific ecological area. Having a healthy phytobiome is critical to crop health,
improved crop yields and quality food. However, crop microbiomes are relatively
under-researched. The UK Crop Microbiome Cryobank project will develop a
unique, exploitable and integrated resource that will provide the biological and
bioinformatic tools to enable the development of solutions to improve soil and
crop health. Six of the UK’s key crops will be the focus and usable outputs will
underpin UK research activity in line with the Biotechnology and Biological
Sciences Research Council (BBSRC) strategic priorities in agriculture and food
security. The project will support three of the UN’s Sustainable-Development
Goals: Zero Hunger, Responsible Consumption, and Production and Life on
Land.

The problem A microbiome is the community of micro-organisms that live together in a


particular ecosystem. Humans, animals and plants all have their own unique
microbiomes.
The plant microbiome is the microbial communities associated with plants, soils
and fauna and can be considered as an essential component of the functional
‘phytobiome’ – a term used to describe a plant’s specific ecological niche (the
plant itself, the environment and its micro and macro-organisms living on and
around the plant). A healthy ‘phytobiome’ is essential for crop health and the
need to improve yields and nutritional quality.

Whilst we understand the importance of healthy microbial communities for better


crop health, research and studies carried out on what has been published is
uncoordinated and fragmented and, as a result, there are no ‘crop microbiome’
resources publicly available. This leads to the problem of research that cannot be
verified and a lack of resources to facilitate further research or the development
of new biological solutions for Agritech.

Having the right tools and resources available will help safeguard future research
and facilitate the sustainable production of the UK’s key food crops. They will
support researchers in their work and provide industry with what they need to
develop biological alternatives to chemical interventions in farming systems.

What we are doing The UK Crop Microbiome CryoBank (UK-CMCB) project will provide a
comprehensive bank of cultures, resources and information to help facilitate
research into optimizing plant yields using a sustainable agricultural approach.

As a lead on this project, CABI will manage the research project, cryopreserve
the samples and host the CryoBank. We will also coordinate the establishment of
the publicly available AgMicrobiome Base.

The project will establish a cryopreserved and characterized crop microbiome


resource to underpin UK and international crop research, building on the UK
Agritech capability provided through the Centre for Crop Health and Protection
(CHAP).

The resource will provide a facility for researchers to source data and samples
for their work, including living microbial material from the rhizoplane and genomic
sequences from different microbiome environments (as axenic cultures,
microbiome samples and in 96 well plates).

This will enable soil scientists and plant researchers to assess and compare their
work against validated datasets generated by the project.

The focus will be on the microbiomes of six major UK crops (barley, oats, oil
seed rape, potato, sugar beet and wheat) from three different soil types obtained
from across the UK.

The CryoBank will also be of interest to the general public. Through CABI and
the projects’ partners, research will be presented to the public as well as ways in
which they can engage with this unique resource.

Results so far The key projected outputs of the UK-CMCB project will include:

A cryopreserved resource of characterized material from crop


microbiomes with a prioritized collection strategy, consisting of fungi,
bacteria and ‘whole’ microbiome samples. Frozen samples will also be
made publicly available through the CABI database and linked to genomic
data
Robust methodologies, which will be available to researchers, for
collection and storage of intact microbial communities in environmental
samples and extracts of total DNA
Using ‘state-of-the-art’ cryopreservation technology to develop advanced
cryopreservation regimes which will enhance the capability of sustainably
maintaining the resource in a genotypically and phenotypically stable state
Genomic characterization of samples for assessing microbial diversity
(including symbionts, endophytes, pathogens), from whole community
taxonomies (bacteria, fungi, viruses) to individual isolate genomes, will be
undertaken
Finding new biological-based products for the Agritech industry which will
be demonstrated to the user community and will take advantage of Plant
Growth Promoting Rhizobacteria (PGPR) isolation and synthetic
community construction
A validated sequence resources database, ‘AgMicrobiome Base’ linked to
European Bioinformatics Institute (EBI) tools and data and available to the
Agritech sector and researchers, including model organisms and novel
product outputs.

The resource will begin to be made available during the first year of the project. It
will then expand throughout the duration of the project.

AgMicrobiome Base will be made available in late Spring 2021.

Updates on the project can be found via Twitter (@PMicrobiome) and the
scientific literature.

Donors Biotechnology and Biotechnology Sciences Research Council - UK Research


Innovation

Partners Rothamsted Research (Dr Tim Mauchline), Scotland’s Rural College (Prof Nicola
Holden), John Innes Centre (Dr Jake Malone), James Hutton Institute (Dr Sue
Jones)

CABI Project Manager Matthew Ryan

https://www.cabi.org/what-we-do/cabi-
projects/

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