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    E GQ VZB 001 17OHP 2009 v2

    Uploaded by

    Nghi Nguyen

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    E GQ VZB 001 17OHP 2009 v2

    Uploaded by

    Nghi Nguyen
    12 views2 pages

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    E_GQ_VZB_001_17OHP_2009_v2

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    12 views2 pages

    E GQ VZB 001 17OHP 2009 v2

    Uploaded by

    Nghi Nguyen

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    NEONATAL 17-OHP

    Screening Elisa assay

    Enzyme immunoassay
    DR Us
    for the titration of IED ing
    17 Alpha-hydroxyprogesterone B
    SP LOO
    in newborns OT D
    S

    Indications
    Features
    ∎∎ Quantitative determination of
    ∎∎ Convenient transport and
    17 Alpha-Hydroxyprogesterone in
    good stability of the samples
    blood samples dried on
    Schleicher & Schuell 903 ∎∎ Accurate, sensitive and
    filter paper. specific assay
    ORDERING INFORMATION
    ∎∎ This kit is particularly suitable ∎∎ Non-isotopic method
    Code : E-GQ-192 for screening of congenital
    Package Size : 192 tests/kit hyperplasia (CAH) in newborns. Performance
    Code : E-GQ-1920 characteristics
    Package size : 1920 tests/kit
    ∎∎ Detection limit : 1.82 ng/mL
    ∎∎ Measuring range :
    from 0 to 200 ng/mL
    Kit contents
    Quantity
    Reagents 192 tests 1920 tests

    Microplates (breakable strips) 2 x 96 20 x 96

    Calibrators 1-8 1 aluminium pouch 3 aluminium pouches

    17-OHP conjugate 1 x 0.45 ml 10 x 0.45 ml

    Extraction buffer 2 x 30 ml 1 x 500 ml

    Controls 1 aluminium bag 3 aluminium bags

    Washing Solution 1 x 55 ml 1 x 500 ml

    Chromogenic substrate 1 x 30 ml 1 x 250 ml


    Zentech s.a.
    Blocking Reagent 1 x 30 ml 1 x 250 ml
    Liège Science Park
    Avenue du Pré-Aily,10
    B-4031 Angleur • Belgium
    Tel : +32 (0)4 361 42 32
    Fax : +32 (0)4 367 00 63
    info@zentech.be
    http://www.zentech.be
    E-GQ-VZB-001 / 2012-12-01
    NEONATAL 17-OHP
    Screening Elisa assay
    Principle of the test
    The 17-OHP Screening ELISA is an enzyme immunoassay to quantitate human 17 Alpha-Hydroxy-
    progesterone (17-OHP) using dried blood spots.
    The 17-OHP Screening ELISA is a competitive ELISA. The strips are coated with an anti-17-OHP
    antibody that capture 17-OHP present in the sample. Dried blood spots are incubated with the ex-
    traction buffer on a shaker for 1 hour at room temperature prior to overnight incubation at 4 °C.
    The extraction buffer contains a 17-OHP molecule conjugated to horseradish peroxidase (HRP).
    The 17-OHP peroxidase conjugate is allowed to compete with 17-OHP present in the sample. The
    following complexes are formed :
    Rabbit anti-17-OHP * 17-OHP in sample è Rabbit anti-17-OHP * 17-OHP peroxidase conjugate
    After a washing step, the immunocomplex is detected by reduction of 3.3’-5.5’-tetramethylbenzi-
    dine (TMB) by HRP. The development of a blue colour is inversely proportional to the amount of
    antigen in the sample or calibrator. The enzymatic reaction is stopped by addition of sulfuric acid
    and absorbance at 450 nm is read using an ELISA microtiter plate reader.

    Analytical performances
    Precision
    The intra-assay imprecision has been tested with 2 samples containing various amounts of
    17-OHP in 8 replicates.
    17-OHP
    (ng/ml) Standard Deviation CV (%)

    Sample 1 7.49 0.809 10.8


    Sample 2 52.84 4.558 8.63

    The inter-assay precision has been tested in 3 separated assays with 2 samples containing various
    amounts of 17-OHP, each sample tested in 8 replicates.

    17-OHP
    (ng/ml) Standard Deviation CV (%)

    Sample 1 7.8 0.3 3.4


    Sample 2 55.8 11.6 20.8

    Recovery
    Known amounts of 17-OHP were added to sample to determine recovery of the assay.
    17-OHP 17-OHP
    Added (ng/ml) Recovered (ng/ml) Recovery (%)

    Zentech s.a.
    Liège Science Park Sample 1 200 213.98 107
    Avenue du Pré-Aily,10 100 109.96 110
    B-4031 Angleur • Belgium 25 23.06 92.2
    Tel : +32 (0)4 361 42 32
    50 55.11 110.2
    Fax : +32 (0)4 367 00 63
    info@zentech.be
    http://www.zentech.be
    E-GQ-VZB-001 / 2012-12-01

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