Wa0018.

CHEMISTRY
INVESTIGATORY
PROJECT
NAME- ............................
CLASS- XII SCIENCE
ROLL NO-
SUBJECT- CHEMISTRY
TOPIC-STUDY OF QUANTITY
OF CAESIN PRESENT IN
DIFFERENT MILK SAMPLES
This is to certify that ................................................ of class
XII (Science) of Kendriya Vidyalaya Kalaikunda has
completed the InvestigatoryProject in Chemistry titled
“Study Of Quantity of Casein present in Different Samples
of Milk” himself under the supervision and guidance of
Chemistry Teacher during the year 2018-19. The progress
of the project has been continuously reported and has
been in my knowledge consistently.
Signature of Signature of External Signature of subject

Principal Invigilator Teacher
First of all, I am deeply thankful to my Chemistry
teacher ............................................ for extending his valuable
and scholarly g uid a nc e throug hout the p rep a ra tion of this p
rojec t. I wish to extend my sinc ere tha nks to
Mr. .......................................................... resp ec ted Princ ip a l,
Kend riya Vid ya la ya Birb hum for his c ontinuous motiva tion a
nd mora l sup p ort for c omp leting the work.
I a m a lso tha nkful to a ll my resp ec ted tea c hers, lib ra
ry sta ff, offic e sta ff a nd sta ff memb ers of the C hemistry L
a b ora tory of the sc hool.
I a m a lso tha nkful to the tea c hers a nd sta ff members of
the department of Chemistry for
extend ing their huma nita ria n a nd sc hola stic a ssista nc e
in find ing out the solutions of some q uery d uring my work.
Last but no the least I am indebted to my friend s, c la
ssma tes a nd p a rents for enc oura g ing a nd never let me
d own in my c onfid enc e while c omp leting the p rojec t.
INTRODUCTION
What is Casein ?
Casein is the protein found in all mammals’ milk. Mammals
include cow, goat, sheep, yak, buffalo, camel and humans.
Milk is a complete diet as it contains minerals, vitamins,

proteins, Carbohydrates, Fats And Water . Average
composition of milk fro different sources is given below :
SOURCE WATER MINERAL PROTEIN FATS CARBOH
OF MILK (%) S (%) (%) YDRATES
(%) (%)
Cow 87.1 0.7 3.4 3.9 4.9
Human 87.4 0.2 1.4 4.0 4.9
Goat 87 0.7 3.3 4.2 4.8
Sheep 82.6 0.9 5.5 6.5 4.5
Casein in the most predominant protein in milk and is a mixed
phosphor protein. Casein has a isoelectric pH of about 4.7 can
be easily separated around this isoelectric pH. It readily
dissolves in dilute acids and alkalies.
Casein is present in milk as calcium caseinate in the form of

micelles. These micelles have negative charge and on adding
acid to milk the negative charges are neutralized.
Ca2 +-Caesinate +2CH3COOH(aq)-Caesein+(CHCOO)2Ca

Natural milk is an opaque white fluid Secreted by the
mammary glands of Female mammal .The main constituents
of natural milk are Protein, Carbohydrate, Mineral Vitamins,
Fats and Water and is a complete balanced diet .Fresh milk is
sweetish in taste .However, when it is kept for long time at a
temperature of 5 degree it become sour because of bacteria
present in air. These bacteria convert lactose of milk into
lactic
acid which is sour in taste. In acidic conditions casein of milk
separate out as precipitate. When the acidity in milk is
sufficient and temperature is around 36 degree, it forms
curd.
APPLICATIONS:
In addition to being consumed in milk casein is used in the
manufacture of adhesives, binders, protective coatings,
plastics(such as knife handles and knitting needles), fabrics,
food additives, and many other products.It is commonly used
by body builders as slow digesting whey proteins and also as
an extremely high source of glutamine (post workout).
Another reason, it is used in bodybuilding is because of its
anti-catabolic effect,meaning that casein consumption inhibits
protein breakdown in the body. Casein is frequently found in
non dairy substitutes to improve consistency especially when
melted.
AIM :
To study quantity of casein in different samples of milk.
THEORY :
Milk contains 3% to4% casein suspended in water in colloidal form.It
si precipitated in weakly acidic medium
APPARATUS REQUIRED :
Funnel, funnel stand , glass rod , filter paper, weight box ,
test tubes, pestle and mortar.
CHEMICALS REQUIRED :
(i) Different samples of milk.
(ii) Saturated ammonium sulphate solution.
(iii) 1 % acetic acid solution.
PROCEDURE :
1. Wash the beaker (250 ml) with the distilled water and dry it.
2. Take 20 ml of buffalo’s milk in 250 ml beaker and find its weight.
3. Add 20 ml saturated solution of ammonium sulphate slowly with

stirring. Fat and casein will separate out as precipitate.
4. Filter the above solution and transfer the precipitate in another

beaker.
5. Treat the above precipitate with 30 ml distilled water. Casein

dissolves forming milky solution whereas fat remains as such.
6. Warm the above contents of the beaker to 40 - 45°C on a low

flame. Now, add 1% acetic acid solution drop wise with stirring
when casein gets precipitated.
7. Filter the precipitated casein and wash with distilled water and dry
it.
8. Find the weight of dry precipitate.
9. Repeat the whole experiment with cow’s milk, goat’s milk and
sheep’s milk.
OBSERVATIONS :
Volume of milk taken in each
 case
  20 ml
Weight of milk taken

 W1 g
Weight of Casein isolated


W2 g
Percentage of casein =
Weight of casien
* 100
Weight of Milk
SL.NO Type of Volume of Weight of Weight of Percentage

Milk Milk Taken Milk Casein Of
(ml) ( g) ( g) Casein
1 Buffalo’s 20 23.09 0.632 2.73 %
milk
2 Cow’s 20 35.66 0.55 1.64 %
milk
3 Goat’s 20 23.09 0.77 3.67 %
milk
RESULT :
(i) Different Samples of milk contains different percentage of
casein.
(ii) Highest percentage of casein is present in Goat’s milk.
PRECAUTIONS :
1. Handle apparatus and chemicals carefully.
2. Add ammonium sulphate solution very slowly.
3. Stir milk while a dding chemicals.
4. Do not disturb milk after adding ammonium sulphate

solution and wait some time for fat and casein to precipitate
out.
5. Take the amount readings carefully with digital weighing

machine only.
BIBLIOGRAPHY
 www.wikipedia.co m
 www.encyclopedia.co m
 www.caesine-pro.co m
 www.sciencejournals.co m
 www.icar.nic.i n
 www.zetascience.co m
 www.scribd.co m

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CHEMISTRY

Signature of Signature of External Signature of subject

Milk is a complete diet as it contains minerals, vitamins,

Casein is present in milk as calcium caseinate in the form of

Ca2 +-Caesinate +2CH3COOH(aq)-Caesein+(CHCOO)2Ca

2. Take 20 ml of buffalo’s milk in 250 ml beaker and find its weight.

3. Add 20 ml saturated solution of ammonium sulphate slowly with

4. Filter the above solution and transfer the precipitate in another

5. Treat the above precipitate with 30 ml distilled water. Casein

6. Warm the above contents of the beaker to 40 - 45°C on a low

8. Find the weight of dry precipitate.

Weight of milk taken

Weight of Casein isolated

SL.NO Type of Volume of Weight of Weight of Percentage

(ii) Highest percentage of casein is present in Goat’s milk.

1. Handle apparatus and chemicals carefully.

2. Add ammonium sulphate solution very slowly.

3. Stir milk while a dding chemicals.

4. Do not disturb milk after adding ammonium sulphate

5. Take the amount readings carefully with digital weighing

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