Rev.

CO012001

Packed Columns for

Ion Chromatography
TSKgel SuperIC-Anion HS
TSKgel SuperIC-AZ
TSKgel SuperIC-AP
TSKgel SuperIC-Anion
TSKgel SuperIC-Cation HS
TSKgel SuperIC-CR
TSKgel SuperIC-A/C

INSTRUCTION
MANUAL
 Safety Precautions
To help protect you and/or your property from potential damage and ensure
personal safety, please read this manual thoroughly before using the product.

[Notational Conventions]
Notation Explanation
Indicates a hazard with a medium level of risk which, if
! WARNING not avoided, could result in death or serious injury.

Indicates a hazard with a low level of risk which, if not

! CAUTION avoided, could result in minor or moderate injury.

! WARNING
Keep away from fire
Not taking proper precautions when using flammable solvents could result in
fire, explosion, or poisoning.

! CAUTION
Use only in well-ventilated areas
In case of insufficient ventilation, flammable and toxic solvents can cause fire,
explosion, or poisoning.
Do not spill solvents
Spillage and leakage can cause fire, electric shock, poisoning, injury, or
corrosion.
Wear appropriate protective gear when cleaning up a spill.
Wear protective eye gear and gloves
Organic solvents and acids should not come into direct contact with the skin.
Handle the package with care
Inappropriate handling may cause rupturing and/or splattering of the product.
Only use this product for its intended use
This product is intended for the separation. Do not use it for any other purpose.
Make sure compounds are safe
Check that the target compounds and solutions after separation and purification
are safe.
Proper disposal
Dispose in accordance with local laws and regulations.

NOTE
Keep this manual with the product for future reference.
Precautions: Shipping Solvents
First Aid Inhalation ･ Move the person to an area with fresh air and rinse
the mouth with plenty of water.
･ Call immediately for medical attention.
Skin exposure ･ Wash the exposed area with plenty of soap and water.
Eye exposure ･ Open the eyes as wide as possible and rinse with
clean water for at least 15 minutes.
･ Call immediately for medical attention.
Ingestion ･ Rinse the mouth with plenty of water.
･ Call immediately for medical attention.
Handling Ventilation ･ Provide adequate air ventilation to keep organic vapor
and concentrations below approved level.
Storage
Container ･ Container may break if not handled with care.
handling
Wear ･ Use solvent-resistant gloves and protective eye gear
appropriate when using this product. Use of a gas mask,
protective additional protective clothing or rubber boots could be
equipment appropriate when handling this product.
Hazardous ･ If any flammable solvents are used for shipping or
substance storage of this product, keep away from fire or open
storage heat sources.
Waste Disposal ･ Dispose in accordance with local laws and regulations.
Disposal methods
General ･ Please pay attention to all safety precautions with
considerations respect to the handling and storage of this product.
Disposal ･ Fumes produced during incineration may contain
precautions nitrogen oxides, sulfur oxides and carbon monoxide.

Shipping solvent for each column : See Section 3. Column Grades and their
Specifications.
Precautions: Packing Material
First Aid Inhalation ･ Move the person to an area with fresh air and rinse the
mouth with plenty of water.
･ Call immediately for medical attention.
Skin exposure ･ Wash the exposed area with plenty of soap and water.
Eye exposure ･ Open the eyes as wide as possible and rinse with
clean water for at least 15 minutes.
･ Call immediately for medical attention.
Ingestion ･ Rinse the mouth with plenty of water.
･ Call immediately for medical attention.
Handling Ventilation ･ Provide adequate air ventilation to keep organic vapor
and concentrations below approved level.
Storage Container ･ Container may break if not handled with care.
handling
Wear ･ Use solvent-resistant gloves and protective eye gear
appropriate when using this product. Use of a gas mask, additional
protective protective clothing or rubber boots could be
equipment appropriate when handling this product.
Hazardous ･ If any flammable solvents are used for shipping or
substance storage of this product, keep away from fire or open
storage heat sources.
Fire ･ Do not expose this chromatographic resin to fire or
precautions open heat sources.
Waste Disposal ･ Dispose in accordance with local laws and regulations.
Disposal methods See below for additional precautions.
General ･ Please pay attention to all safety precautions with
considerations respect to the handling and storage of this product.
Disposal ･ This product can be safely incinerated.
precautions ･ Appropriate nitrogen oxides exhaust emission
precautions should be taken specifically for anion
exchange packing material.

□ TSKgel SuperIC column products contain combustible chromatographic

media based on a co-polymer of vinyl compounds.
 Table of Contents
01. Introduction ………………………………………………………………… 1
02. Prior to Use ………………………………………………………………… 1
03. Column Grades and their Specifications ………………………………… 1
04. Column Configuration ……………………………………………………… 5
05. Operation …………………………………………………………………… 6
06. Installation ………………………………………………………………… 8
07. Storage and Disposal ……………………………………………………… 10
08. Column Cleanup …………………………………………………………… 11
09. Calculation of Theoretical Plate Number and Asymmetry Factor …… 12
10. Quality Specifications and Warranty …………………………………… 13
 Revised in October 2009

1. Introduction
This instruction manual contains crucial information on how to care for and use
these columns in a proper manner, so as to make the most effective use of their
high performance capabilities.
Be sure to carefully read the instructions in this manual prior to use of these
columns.

2. Prior to Use
Be sure to inspect the packaging and closely inspect the column for any signs of
damage prior to use. If any damages are evident, contact your local TOSOH sales
representative at the address listed at the end of this manual.
Please confirm the following documents are included in the package.
1) INSTRUCTION MANUAL 1 copy
2) INSPECTION DATA 1 copy

3. Column Grades and their Specifications

TSKgel SuperIC products consist of 15 different analytical columns and guard
columns as shown in Tables 1 and 2.

Table 1 Analytical Column Grades and their Specifications (1)

Grade TSKgel SuperIC-Anion TSKgel SuperIC-AP

Part No. 0019673 0019840 (15 cm)
0019841 (7.5 cm)
4.6 mm I.D.× 15 cm 4.6 mm I.D.× 15 cm
Dimensions (PEEK1) (PEEK1)
4.6 mm I.D.× 7.5 cm
(PEEK1)
Base material Polystyrene gel Hydrophilic polymer gel
Particle size 5μm 6μm
Functional group Quaternary ammonium ion Quaternary ammonium ion
Capacity ca. 12 meq/L ca. 30 meq/L
Counter ion Borate and carbonate ion Carbonate ion
Shipping solvent Test eluent2 Test eluent2
Applications Anion analysis Anion analysis

− 1 −
 Table 1 Analytical Column Grades and their Specifications (2)

Grade TSKgel SuperIC-AZ TSKgel SuperIC-Anion HS

Part No. 0021444 0022766
Dimensions 4.6 mm I.D.× 15 cm 4.6 mm I.D.× 10 cm
(PEEK1) (PEEK1)
Base material Hydrophilic polymer gel Hydrophilic polymer gel
Particle size 4μm 3.5μm
Functional group Quaternary ammonium ion Quaternary ammonium ion
Capacity ca. 30 meq/L ca. 30 meq/L
Counter ion Carbonate ion Carbonate ion
Shipping solvent Test eluent2 3.8 mmol/L NaHCO3
Applications Anion analysis Anion analysis

Grade TSKgel SuperIC-CR TSKgel SuperIC-Cation HS

Part No. 0021475 0022768
Dimensions 4.6 mm I.D.× 15 cm 4.6 mm I.D.× 10 cm
(PEEK1) (PEEK1)
Base material Polystyrene gel Polystyrene gel
Particle size 3μm 3μm
Functional group Carboxylic acid Carboxylic acid
Capacity 1.0 eq/L or more 1.0 eq/L or more
Counter ion Hydrogen ion Hydrogen ion
Shipping solvent Test eluent2 Test eluent2
Applications Cation analysis Cation analysis

Grade TSKgel SuperIC-A/C

Part No. 0019843
Dimensions 6.0 mm I.D.× 15 cm
(SUS316)
Base material Hydrophilic polymer gel
Particle size 4μm
Functional group Carboxylic acid
Capacity ca. 0.2 eq/L
Counter ion Hydrogen ion
Shipping solvent H 2O
Applications Simultaneous analysis of anion and cation

− 2 −
 Table 2 Guard Column Grades and their Specifications (1)

Grade TSKguardcolumn SuperIC-A TSKguardcolumn SuperIC-AP

Part No. 0019674 0019842
Dimensions 4.6 mm I.D.× 1 cm 4.6 mm I.D.× 1 cm
(PEEK1) (PEEK1)
Base material Polystyrene gel Hydrophilic polymer gel
Particle size 5μm 6μm
Functional group Quaternary ammonium ion Quaternary ammonium ion
Capacity ca. 12 meq/L ca. 30 meq/L
Counter ion Borate and carbonate ion Carbonate ion
Shipping solvent Test eluent2 Test eluent2
Analytical column TSKgel SuperIC-Anion TSKgel SuperIC-AP

Grade TSKguardcolumn SuperIC-AZ TSKguardcolumn SuperIC-A HS

Part No. 0021445 0022767
Dimensions 4.6 mm I.D.× 1 cm 4.6 mm I.D.× 1 cm
(PEEK1) (PEEK1)
Base material Hydrophilic polymer gel Hydrophilic polymer gel
Particle size 4μm 3.5μm
Functional group Quaternary ammonium ion Quaternary ammonium ion
Capacity ca. 30 meq/L ca. 30 meq/L
Counter ion Carbonate ion Carbonate ion
Shipping solvent Test eluent2 3.8 mmol/L NaHCO3
Analytical column TSKgel SuperIC-AZ TSKgel SuperIC-Anion HS

Grade TSKguardcolumn SuperIC-CR TSKguardcolumn SuperIC-C HS

Part No. 0021476 0022769
Dimensions 4.6 mm I.D.× 1 cm 4.6 mm I.D.× 1 cm
(PEEK1) (PEEK1)
Base material Polystyrene gel Polystyrene gel
Particle size 3μm 3μm
Functional group Carboxylic acid Carboxylic acid
Capacity 1.0 eq/L or more 1.0 eq/L or more
Counter ion Hydrogen ion Hydrogen ion
Shipping solvent Test eluent2 Test eluent2
Analytical column TSKgel SuperIC-CR TSKgel SuperIC-Cation HS

− 3 −
 Table 2 Guard Column Grades and their Specifications (2)

Grade TSKguardcolumn SuperIC-A/C

Part No. 0019844
Dimensions 4.6 mm I.D.× 2 cm
(SUS316)
Base material Hydrophilic polymer gel
Particle size 4μm
Functional group Carboxylic acid
Capacity ca. 0.2 eq/L
Counter ion Hydrogen ion
Shipping solvent H 2O
Analytical column TSKgel SuperIC-A/C

1
PEEK : Poly ether ether ketone
2
Test eluent
・TSKgel SuperIC-Anion, TSKguardcolumn SuperIC-A
6.0 mmol/L sodium tetraborate+15 mmol/L boric acid
+0.2 mmol/L sodium hydrogen carbonate
・TSKgel SuperIC-AP, TSKguardcolumn SuperIC-AP
1.7 mmol/L sodium hydrogen carbonate+1.8 mmol/L sodium carbonate
・TSKgel SuperIC-AZ, TSKguardcolumn SuperIC-AZ
6.3 mmol/L sodium hydrogen carbonate+1.7 mmol/L sodium carbonate
・TSKgel SuperIC-CR, TSKguardcolumn SuperIC-CR
2.2 mmol/L methanesulfonic acid+1.0 mmol/L 18-crown 6-ether
・TSKgel SuperIC-Cation HS
3.0 mmol/L methanesulfonic acid+0.4 mmol/L 18-crown 6-ether
+ 0.2 mmol/L L-histidine
・TSKguardcolumn SuperIC-C HS
1.2 mmol/L methanesulfonic acid+0.4 mmol/L 18-crown 6-ether
+0.2 mmol/L L-histidine

When the TOSOH Ion Chromatograph IC-2001 or IC-2010 is used with suppression
mode, one of the following suppressor gels should be installed.
< IC-2001 >
Part No. 0019675 Suppressor gel TSKsuppress IC-A (20 mL gel, for anion)
Part No. 0020310 Suppressor gel TSKsuppress IC-C (20 mL gel, for cation)

− 4 −
 < IC-2010 >
Part No. 0022770 Suppressor gel TSKsuppress IC-A (30 mL gel, for anion)
Part No. 0022771 Suppressor gel TSKsuppress IC-A (60 mL gel, for anion)
Part No. 0022772 Suppressor gel TSKsuppress IC-C (30 mL gel, for cation)
Part No. 0022773 Suppressor gel TSKsuppress IC-C (60 mL gel, for cation)

An analytical column and a guard column should be connected using the following
parts.
Part No. 0016566 Connector (For 1/16” PEEK tubing, package of 2)
Part No. 0017172 PEEK tubing (1/16” O.D., 0.25 mmI.D.× 2 m, 9 cm-cut tubing
should be used.)

4. Column Configuration
The typical configuration of columns are shown in Figures 1-1 and 1-2.

End fitting Column body

End plug Label
Figure 1-1 Analytical Column

Column body End fitting

Label End plug

Figure 1-2 Guard Column

− 5 −
5. Operation
The general operating conditions for each column are shown in Table 3.

Table 3 General Operating Conditions (1)

Grade TSKgel SuperIC-Anion TSKgel SuperIC-AP
TSKguardcolumn SuperIC-A TSKguardcolumn SuperIC-AP
Flow rate Up to 0.8 mL/min Up to 0.8 mL/min
Pressure Analytical : Up to 7.0 MPa Analytical (15 cm) : Up to 7.0 MPa
Analytical (7.5 cm) : Up to 3.5 MPa
Guard : Up to 1.0 MPa Guard : Up to 1.0 MPa
Temperature 25 ∼ 40 ℃ 25 ∼ 40 ℃
pH range pH 2.0 ∼12.0 pH 2.0 ∼12.0
Organic solvent Not recommended Up to 20 vol%

Grade TSKgel SuperIC-AZ TSKgel SuperIC-Anion HS

TSKguardcolumn SuperIC-AZ TSKguardcolumn SuperIC-A HS
Flow rate Up to 0.8 mL/min Up to 1.5 mL/min
Pressure Analytical : Up to 15.0 MPa* Analytical : Up to 25.0 MPa
Guard : Up to 3.0 MPa Guard : Up to 5.0 MPa
Temperature 25 ∼ 40 ℃ 25 ∼ 40 ℃
pH range pH 2.0 ∼12.0 pH 2.0 ∼12.0
Acetonitrile, Methanol : 100 % Acetonitrile, Methanol : 100 %
Organic solvent
available available
*Up to 10.0 MPa when using eluent contains organic solvent.

Grade TSKgel SuperIC-CR TSKgel SuperIC-Cation HS

TSKguardcolumn SuperIC-CR TSKguardcolumn SuperIC-C HS
Flow rate Up to 0.8 mL/min Up to 1.2 mL/min
Pressure Analytical : Up to 15.0 MPa Analytical : Up to 25.0 MPa
Guard : Up to 3.0 MPa Guard : Up to 5.0 MPa
Temperature 25 ∼ 40 ℃ 25 ∼ 40 ℃
pH range pH 1.0 ∼5.0 pH 1.0 ∼5.0
Organic solvent Not recommended Not recommended

− 6
2 −
 Table 3 General Operating Conditions (2)
Grade TSKgel SuperIC-A/C
TSKguardcolumn SuperIC-A/C
Flow rate Up to 0.6 mL/min
Pressure Analytical : Up to 7.0 MPa
Guard : Up to 2.0 MPa
Temperature 25 ∼ 40 ℃
pH range pH 2.0 ∼12.0
Acetonitrile : Up to 20 %
Organic solvent
(Alcohols are not recommended)

CAUTION : Leaks at the connection

M
 ake sure that there are no leaks at each of the connections in ion
chromatograph.

CAUTION : Pressure limitations of IC-2001

The IC-2001 has maximum pressure limit of 15 MPa.
All columns should be used up to 15 MPa on the IC-2001.

CAUTION : Column installation onto the ion chromatograph

Columns : TSKgel SuperIC-AZ, TSKgel SuperIC-Anion HS, TSKgel SuperIC-CR
and TSKgel SuperIC-Cation HS
S
 ince these columns have higher pressure ratings, make sure that there are no
leaks at each connection, especially between the pump and the column.
In the IC-2001, when column temperature is less than 40 ℃, the eluent should
be run at a flow rate at up to 0.3 mL/min to prenent activation of the pressure
limiter sensor of the system.

CAUTION : Preparation of eluent

1) M ake sure the properties of chemicals for the eluent by referring to the
Material Safety Data Sheet (MSDS).
2) Chemicals, solvents and deionized water used to prepare the eluent should be
of the highest purity available.
3) To maintain reproducibility it is recommended to first prepare the eluent in a
10-fold concentrate and then dilute appropriately before to use.

− 7
1 −
 CAUTION : Crown ether containing eluent
C rown ether containing eluents are used for the TSKgel SuperIC-CR and
TSKgel SuperIC-Cation HS columns. If there is a change in the composition of
the eluent containing crown ethers, it may take several minutes to equilibrate
the column.
[Example]
I f you replace the eluent comprised of 2.2 mmol/L methanesulfonic acid
+1.0 mmol/L 18-crown 6-ether with that of 2.2 mmol/L methanesulfonic acid
+0.5 mmol/L 18-crown 6-ether (or vise-versa) or the elution of 2.2 mmol/L
methanesulfonic acid+1.0 mmol/L 18-crown 6-ether with that of 2.2 mmol/L
methanesulfonic acid+2.0 mmol/L 18-crown 6-ether (or vise-versa), do not start
the analysis until at least 60 min at 0.7 mL/min equilibrium time has been run.

6. Installation
6-1 Parts for Connection
Part No. 0016566 Connector (For 1/16” PEEK tubing, package of 2)

6-2 Flow Direction

The flow direction of the eluent is determination by the direction of the arrow show
on the label attached to the column.

6-3 Installation
1) Take the column from the package and remove the end plugs from the column.
2) Start the pump until the eluent leaks flows of the outlet tubing that is connected
to the column. (Leakage eluent should be removed from the inlet tubing.)
3) Connect the guard column and the analytical column sequentially to the tubing,
making sure the flow direction is the same as that indicated on the label and then
pump the eluent at a half of the intended analysis flow rate.
4) After making sure there are no leaks at any of the connections, shut the door of
column oven.
5) After making sure the column temperature reaches the set point, adjust the pump
to run at the analysis flow rate.
6) Equilibrate the column with the eluent before starting analysis.

CAUTION : Switching to another analysis mode

B
 efore switching to another analysis mode (anion or cation), rinse the flow lines

− 8
2 −
 in the system with 80 % acetonitrile.
( Flow rate : 0.5 mL/min, Time for rinse : 30 min or more)

6- 4 Flow Diagram
F low diagrams of the suppression and non-suppression modes are shown in
Figures 2 and 3.

Injector Suppressor

Degasser & Pump Analytical column Drain

Detector
Eluent Guard column

Figure 2 Suppression Mode

Injector

Degasser & Pump Analytical column Drain

Detector
Eluent Guard column

Figure 3 Non-suppression Mode

6-5 Sample Preparation

6-5-1 Elimination of Insoluble Matters
It is highly recommended that all precipitate is removed from the sample. Although
some precipitate is not visible to the naked eye, by centrifuge or use a filter to avoid
contamination of the column.

6-5-2 Elimination of Hydrophobic Compounds

To prevent accumulation of hydrophobic compounds on the packing material, use a
commercially available disposable ODS column, prior to the analytical column.

6-5-3 Dilution of Sample Solution

If the ionic strength of the sample solution is too high (over 100 ppm per ion),

− 9
1 −
overloading of the column may occur which will reduce the accuracy of the
quantitative analysis.
In this case, the sample solution must be diluted to an appropriate ionic strength
with sample buffer or deionized water.

7. Storage and Disposal

Always keeps the column hydrated with storage solvent by attaching the end fittings
to the column. Store the column at room temperature.

7-1 Column Storage

7-1-1 Storage Solvent
For the preparation of storage solvent, see “CAUTION : Preparation of eluent (Page 7)“.
・TSKgel SuperIC-Anion, TSKguardcolumn SuperIC-A,
TSKgel SuperIC-AP, TSKguardcolumn SuperIC-AP,
TSKgel SuperIC-AZ, TSKguardcolumn SuperIC-AZ,
TSKgel SuperIC-CR, TSKguardcolumn SuperIC-CR,
TSKgel SuperIC-Cation HS, TSKguardcolumn SuperIC-C HS
Always keep the column hydrated with eluent.
・TSKgel SuperIC-Anion HS, TSKguardcolumn SuperIC-A HS
Keep the column in eluent buffer if it will be used again within a week.
If the column will not be used for more than a week, it should be store in filled
with 3.8 mmol/L NaHCO3.
・TSKgel SuperIC-A/C
K
 eep the column in eluent buffer if it will be used again within a week.
If the column will not be used for more than a week, it should be store in
deionized water.
Solvent replacement conditions
Solvent : H2O (Deionized water)
Flow rate : 0.3 mL/min
Temp. : Less than 40 ℃
Flow time : 30 min or more

7-1-2 Storage Temperature

Store the column at 15 ~ 30 ℃. If the column is stored less than 0 ℃, it may freeze
and result in degradation of performance.

− 10 −
7-2 Disposal
The column body should be discarded as plastics or stainless steel.
Packing materials in the column should be disposed as plastics.

8. Column Cleanup
8-1 Removal of Polyvalent Electrolytes
Prolonged operation with a complex mixture of ions in the sample may lead to
gradual accumulation of polyvalent electrolyte compounds in the column.
This is evidenced by changes in chromatographic behavior and apparent loss of ion
exchange capacity.
Adsorbed materials can be stripped from the column by flushing with the following
cleaning solvents. (The effectiveness of this procedure will depand on the properties
of the individual contaminants.)
The column should be cleaned for more than an hour at a half of the operating flow
rate shown on the INSPECTION DATA sheet.
Typical cleaning solvents
・TSKgel SuperIC-Anion, TSKguardcolumn SuperIC-A,
TSKgel SuperIC-AP, TSKguardcolumn SuperIC-AP,
TSKgel SuperIC-AZ, TSKguardcolumn SuperIC-AZ,
TSKgel SuperIC-Anion HS, TSKguardcolumn SuperIC-A HS
20 mmol/L sodium carbonate+20 mmol/L sodium hydrogen carbonate
・TSKgel SuperIC-CR, TSKguardcolumn SuperIC-CR,
TSKgel SuperIC-Cation HS, TSKguardcolumn SuperIC-C HS
10 mmol/L methanesulfonic acid+1.0 mmol/L 18-crown 6-ether
・TSKgel SuperIC-A/C, TSKguardcolumn SuperIC-A/C
5 mmol/L sulfuric acid or 0.1 % phosphoric acid

8-2 Removal of Hydrophobic Compounds

Flush the packing material in the column with a cleaning solvent containing organic
solvents. (Cleanup effect will depend on properties of contaminants.)
The column should be cleaned for more than an hour at a half of the operating flow
rate shown in the INSPECTION DATA sheet.

CAUTION : Applicable solvent

Applicable organic solvent (i.e., acetonitrile or methanol) depends on the type of
the column. Make sure to use the organic solvents recommended for each

− 11 −
 column by referring to Table 3.
Please note, no organic solvents can be run on the TSKgel SuperIC-Anion,
TSKgel SuperIC-CR and TSKgel SuperIC-Cation HS columns. The use of a
disposable pretreatment column or guardcolumn is highly recommended to
prevent damages to the column.

9. Calculation of Theoretical Plate Number and Asymmetry Factor

The theoretical plate number (N) and the asymmetry factor (As) as well as their
chromatographic conditions for each columns are as shown on the INSPECTION
DATA sheet.

9-1 Method of Calculating Theoretical Plate Number

Ve

h
W 1/2
1/2h

Injection
Figure 4 Method of Calculating Theoretical Plate Number

The theoretical plate number (N) of a column is calculated by the half width method
shown in Figure 4 and the following equation.
N = 5.54 (Ve/W1/2)2
Ve : Elution time (min)
W1/2 : Peak width (min) at one-half the peak height
h : Peak height

− 12 −
9-2 Method of Calculating Asymmetry Factor

Ve

h
a b
1/10h

Injection
Figure 5 Method of Calculating Asymmetry Factor

The asymmetry factor (As) of a column is calculated by the 1/10h method.

As = b/a

10. Quality Specifications and Warranty

10-1 Conditions for Inspection Data
1) TSKgel Super IC-Anion
Eluent : 6.0 mmol/L sodium tetraborate+15 mmol/L boric acid
+0.2 mmol/L sodium hydrogen carbonate
Flow rate : 0.8 mL/min
Temp. : 40 ℃
Sample : SO42- ion (5 mg/L)
Injection vol. : 30μL
Suppressor gel : TSKsuppress IC-A
Instrument : TOSOH Ion Chromatograph IC-2001
2) TSKgel SuperIC-AP
Eluent : 1.7 mmol/L sodium hydrogen carbonate
+1.8 mmol/L sodium carbonate
Flow rate : 0.8 mL/min
Temp. : 40 ℃
Sample : SO42- ion (5 mg/L)
Injection vol. : 30μL
Suppressor gel : TSKsuppress IC-A
Instrument : TOSOH Ion Chromatograph IC-2001
3) TSKgel SuperIC-AZ
Eluent : 6.3 mmol/L sodium hydrogen carbonate
+1.7 mmol/L sodium carbonate

− 13 −
 Flow rate : 0.8 mL/min
Temp. : 40 ℃
Sample : SO42- ion (5 mg/L)
Injection vol. : 30μL
Suppressor gel : TSKsuppress IC-A
Instrument : TOSOH Ion Chromatograph IC-2001
4) TSKgel SuperIC-Anion HS
Eluent : 3.8 mmol/L sodium hydrogen carbonate
+3.0 mmol/L sodium carbonate
Flow rate : 1.5 mL/min
Temp. : 40 ℃
Sample : SO42- ion (5 mg/L)
Injection vol. : 30μL
Suppressor gel : TSKsuppress IC-A
Instrument : TOSOH Ion Chromatograph IC-2010
5) TSKgel Super IC-CR
Eluent : 2.2 mmol/L methanesulfonic acid
+1.0 mmol/L 18-crown 6-ether
Flow rate : 0.7 mL/min
Temp. : 40 ℃
Sample : Na+ and NH4+ ion (2 mg/L each)
Injection vol. : 30μL
Suppressor gel : TSKsuppress IC-C
Instrument : TOSOH Ion Chromatograph IC-2001
6) TSKgel Super IC-Cation HS
Eluent : 3.0 mmol/L methanesulfonic acid
+0.4 mmol/L 18-crown 6-ether+0.2 mmol/L L-histidine
Flow rate : 1.2 mL/min
Temp. : 40 ℃
Sample : Na+ ion (2 mg/L) and K+ ion (5 mg/L)
Injection vol. : 30μL
Suppressor gel : TSKsuppress IC-C
Instrument : TOSOH Ion Chromatograph IC-2010
7) TSKgel Super IC-A/C
Eluent : 6.0 mmol/L 18-crown 6-ether+0.45 mmol/L 5-sulfosalicylic acid
+5.0 mmol/L L-tartaric acid+5 % acetonitrile
Flow rate : 0.6 mL/min

− 14 −
 Temp. : 40 ℃
Sample : Cl- ion (2.95 mg/L) and Na+ ion (0.38 mg/L)
Injection vol. : 30μL
Instrument : TOSOH Ion Chromatograph IC-2001

10-2 Quality Specifications

Table 4 Quality Specifications

Grade TSKgel SuperIC-Anion TSKgel SuperIC-AZ

Part No. 0019673 0021444
Plates 9,000 or more (SO42-) 10,000 or more (SO42-)
Asymmetry 1.1∼2.0 (SO42-) 0.8∼1.5 (SO42-)

Grade TSKgel SuperIC-AP TSKgel SuperIC-AP

Part No. 0019840 0019841
Plates 8,000 or more (SO4 ) 2-
4,000 or more (SO42-)
Asymmetry 0.8∼1.5 (SO42-) 0.8∼1.5 (SO42-)

Grade TSKgel SuperIC-Anion-HS

Part No. 0022766
Plates 8,000∼11,000 (SO42-)
Asymmetry 0.9∼1.4 (SO42-)

Grade TSKgel SuperIC-CR TSKgel SuperIC-Cation HS

Part No. 0021475 0022768
Plates 8,000∼16,000 (Na+) 4,500∼7,000 (Na+)
0.9∼1.4 (Na+)
Asymmetry 0.9∼1.6 (Na+)
2.4∼3.6 (K+)
Rs 5.6 or more (Na -NH4 )
+ +
−

Grade TSKgel SuperIC-A/C

Part No. 0019843
Plates 5,000 or more (CI-), 14,000 or more (Na+)
Asymmetry 0.8∼2.0 (CI-), 0.8∼2.0 (Na+)

− 15 −
10-3 Warranty
Check the appearance of the column and test its performance according to Section 9
within two weeks of the receipt of the column.
If the quality specifications in Table 4 can not be obtained or the column has been
damaged during transportation, contact a TOSOH representative within two weeks.
TOSOH will replace the column free of charge.
Note that column lifetime is not guaranteed.
No column should be returned to TOSOH without TOSOH’s prior consent.
The specifications of these columns may be improved without notice.

− 16 −
 TOSOH CORPORATION
BIOSCIENCE DIVISION
Shiba-Koen First Bldg.
3-8-2 Shiba, Minato-ku, Tokyo 105-8623, Japan
Phone: +81-3-5427-5180 Fax: +81-3-5427-5220
Web site: http://www.tosoh.com/Products/SeparationPurification/
HPLC database: www2.tosoh.co.jp/hlc/hlcdb.nsf/StartE?OpenForm

TOSOH BIOSCIENCE LLC

156 Keystone Drive, Montgomeryville, PA 18936, USA
Phone: +1-215-283-5000 Fax: +1-215-283-5035
E-mail: Sales&marketing.sep@tosohbioscience.com
Web site: www.tosohbioscience.com

TOSOH BIOSCIENCE GmbH

Zettachring 6, 70567 Stuttgart, Germany
Phone: +49-711-132570 Fax: +49-711-1325789
E-mail: Info.sep.eu@tosohbioscience.com
Web site: www.tosohbioscience.com

TOSOH (SHANGHAI) CO., LTD.

BIOSCIENCE DIVISION
Room B205, Gienkeeplas Center,
No. 1221 Hami Road, Shanghai 200335, China
Phone: +86-21-5219-2751 Fax: +86-21-5219-2781
E-mail: Info@tosoh.com.cn
Web site: www.tosohshanghai.com

HLC, TSK-GEL, TSKgel, TSKgel SuperMultipore, BioAssist, Enantio, PStQuick, Enviropak,

TOYOPEARL, ToyoScreen, TOYOPEARL GigaCap, TOYOPEARL MegaCap, TOYOPEARLPAK
and TOYOPAK are registered trademarks of TOSOH CORPORATION in Japan and other countries.

This manual may not be reprinted or copied in whole or in part without written consent of TOSOH
CORPORATION. The contents of the manual are subject to change without notice.

Printed in Japan
T0910-500