Instrumental Method Analysis - PT711
Instrumental Method Analysis - PT711
Instrumental Method Analysis - PT711
1.
nolson.k,alkor,(aoi).
poiniples
d.
and molecular
and teehnies f Biockemistry
Biday(8 pu:
CambridgeUniaity
ado). nr
york
GR. s.k.3.
and Anand,
chatwal,
Mehotsofchemical . (a003)
Tnstrumental
Analysh page No -(2-62-2.69)
-pyoffomam,
3 Thsteevtal uethodof Analysis
s.
prof.s. patil, (br).
prof. 5-D Borhde -
Salunke,
py publcoteon,pageNo - (264-273).
ston
Concky
In Corelusion,
ipnaoehonge
chxomateguohg
ofim-oehange
priniples
.Rrelis
interoctigns
oinsemtation
specialiaad tat
separatea
ns
lnu m samlebasedntin ehage charet
a
în
ties.Rei
cde
methodfindo applications
ialds,rnelusughiocemisty,anuirm
Vaios csewch
enablirg
,phaNmaeuties
s ienee
mantal
Španate and anay ionswitan
ersatlityand mateit
preeesio t amd
le tod fa undetanding
indipes inboh
diese
dharacterizing
substaneon
tting.
seeh and inatril
Siuated na suitLlo
sulabe call.
Plouo ensor
Thee Consists
of sealadnto a
tuoo aloctrodes gassflow call.
6.Jatabstem:
InToutine ohere
analysis ,
no automation is
computig
pre-progommad intagotor.
neaded,a sa more
Buficint.for higer contsolevels
may be
adatastation
ntelligont such
denicey necesang, as
or miniComputen.
f
Applcaions tonGrchangechaomatrgaphy.
sAn impostaat
of anino
chxomatngraghy
useofton-aoehange
acid mitunes.
n anolysie
the outine
of hasd coaten.
8oftening
ofnueleic ocid.
Toanayaebasecompastion
y to Sopanoteproteinmajstunes.
( meas Qhe oddities
e in food onddrug
Sarmple.
tn He somple.
the natalions
fe Comecntrote
f loator.
9Deminenalizateon
3.Colemns:
Depending
onta we and ana of
ultinate
be stanles
appication,
the Colemn matenialmay
stasl,tonùem glas such as
ment plastie
(e
Peek. lolenn ean An diameton fromnabout
to 5o em
a mm to
5 andin lenth from 33
m
e
conductiity euse.
iscommanly
dattors Te
elactvica
of eonduetity
electrica
& the simplast
deteator
Sensor Hhe
aleciodes
and Consiss
ofonly two
f allhe dateetos
sensors
eluent
Jataproui
Traebae
of te
to be one.
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mast impostont Componantintee sytem nohich
eonstantow of te
a eontinunu
has to psovide
Columnand datactor.
sluent4haoh he ac mzeetor,
mtroduetioncan
Cor bo.
be aceom
2.Tngectax:
se
Sample
in Vaniausoays
plihed
vahe.linuid
. Re amethod
singlet
a ampleemay ke
to
injecion
n
,o
groupo
bownd fumetional
thesecovalntly
chargedions
OPpositely
ase
grougs
bounded(mobile
Counton
wth likechangeisna
non),ohich will be aochansed
moxethan the
inte óamplehaingchangemognitude
qons boendeddo he matsik.
1fanioeayochonge
hus, išperformad.
chrormatograghy
changed sompa Componant wd
negatevaly ivitoect
mose oith he stotàonayphaseand willke eochanged
aheady boenladto he matiis
for(ikechangedions
of Tonexchangechsormatagsaphy
Imstremertotaon
inecor,
perp,
includes:
Tc nstumentotion
Täpical f data
and cocorden
colera,Suresos,
datecer
getem.
Theionarchange hao tuooges
caromatogray
of eochongehs i.c. and onionic
catioaie eahasges.
(liqui)
Mobile phase :
phazComposad :
Samelemaile
chaqedor pelau
SampleContaining
mdlecle.
Bufer.
paue compoud
tationany f:
Resin changedmeecules.
witth Covalontly
Typsof Toneschongen
chsomatosahg
•Anion exchongeas
aae to a
attrocted
changpd molecudes
positively
chagad solid
negativaly Gutost.
Cenon uaad
,6ufate
are are S-resin
catiooeychangehesins
deriatis ;
and cM Kesins. deried
Carboxylate ions.
4 Cotion
Gxchangess
changodmolacules
is attroctod
to a positively
Ngatiay ued axchange
chasgedsoidsuport. Comnonly
Q- resm,
Resinsane Q- resin,
a
a amineond
Quatemany
peAE en.DiethAmino thone.
ToprC:
Ton Exehagechwomatography
and apflicotion.
tratrunentatin
principle,
Lonexchangechenatagsiy
Definition
by ohick
y a procus
Ton chsomatogatlsg
exchange
changed onscan he
f similas
miotere
dig
aecos
don- n
eehongeesin
to their eatie
affinitis.
nohid
ions
ochanges
prorestis ionepehage
he mostcomron
and cgonic
• ae alnostmsubeinwater
They
tetachlocde
Buch as beneene,Carbon
Solyezts
etherete.
Complyo n natue ,einfodtthey
oymenie.
have actieos counterions hat will
marks:
Full 25 ..
Q023-24....
Session:
Organization 10marks
ofthereport:
level skill:
of writing S marks
TonExchanggcheonatoqrcgl
mstrmentatene
ASSINGMENT TOPICT inciple,
REPORT VWRITING/
"Sglizati
SUBMITTED BY
NAME OF THE STUDENT: Semanbasba
ROLLNO: 20101920091
OFQ020-2|
NO:202o10201910013
REGISTRATION
SEMESTER: 74
PAPER NAME: Tnstrumertal Theory.
ethods Anaysis
PAPER CODE: PT-74
SIGNATURE
OF THE STUDENT:
4/os/2023
Dateofsubmission: