Scribd Logo
Upload

Welcome to Scribd!

  • 72 views

    Exp. 10 Urinalysis

    Uploaded by

    Risha Pedroso
    This document outlines an experiment on urinalysis. The introduction provides background on urinalysis and its importance for detecting diseases. The objectives, materials, procedure, results, and conclusion sections provide the structure and steps to analyze urine samples and understand the significance of various physical and chemical characteristics and constituents. The goal is to learn how to perform urinalysis and interpret the results to gain health insights.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Exp. 10 Urinalysis

    Uploaded by

    Risha Pedroso
    72 views5 pages
    This document outlines an experiment on urinalysis. The introduction provides background on urinalysis and its importance for detecting diseases. The objectives, materials, procedure, results, and conclusion sections provide the structure and steps to analyze urine samples and understand the significance of various physical and chemical characteristics and constituents. The goal is to learn how to perform urinalysis and interpret the results to gain health insights.

    Original Title

    Exp.-10-Urinalysis

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document outlines an experiment on urinalysis. The introduction provides background on urinalysis and its importance for detecting diseases. The objectives, materials, procedure, results, and conclusion sections provide the structure and steps to analyze urine samples and understand the significance of various physical and chemical characteristics and constituents. The goal is to learn how to perform urinalysis and interpret the results to gain health insights.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    72 views5 pages

    Exp. 10 Urinalysis

    Uploaded by

    Risha Pedroso
    This document outlines an experiment on urinalysis. The introduction provides background on urinalysis and its importance for detecting diseases. The objectives, materials, procedure, results, and conclusion sections provide the structure and steps to analyze urine samples and understand the significance of various physical and chemical characteristics and constituents. The goal is to learn how to perform urinalysis and interpret the results to gain health insights.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 5

    Name: _____________________________ Date Performed: ____________

    Year and Course: ____________________ Date Submitted: ____________


    Subject title: ________________________ Code number: _______________
    Name of Professor: ___________________ Score: ______________________

    Exercise no. 10
    URINALYSIS

    I. INTRODUCTION

    Urinalysis: A test that is done in order to analyze urine. Because toxins and
    excess fluid are removed from the body in urine, analysis of urine can provide
    important health clues. Urinalysis can be used to detect certain diseases, such
    as diabetes, gout, and other metabolic disorders, as well as kidney disease. It can also
    be used to uncover evidence of drug abuse. Accurate urinalysis may require a 'clean
    catch' of urine. Before a person gives a urine sample, he or she should drink plenty of
    fluids and wait until 1 or 2 seconds into the flow of urine before catching the urine in
    the receptacle. For some tests it is important to get the first urine of the day, which
    contains the highest concentration of toxins and other substances to be analyzed. For
    other tests, a 24-hour collection of urine may be needed.

    II. OBJECTIVES:
    At the end of the experiment, each student will be able to:

    1. ___________________________________________________
    ___________________________________________________

    2. ___________________________________________________
    ___________________________________________________

    III. MATERIALS
    25 – test tubes, test tube rack, test tube brush, test tube holder, tripod, alcohol
    lamp, wire gauze, 5-10ml graduated cylinder, 100ml graduated cylinder, 500ml
    beaker, 5-100ml beaker, 5-medicine dropper, blue and red litmus paper, pH
    paper, pH meter, Hydrometer

    5ml 5% acetic acid, 5ml Benedict’s solution, 5ml Fehling’s A and B, 5ml
    Glacial acetic acid, 2ml ammonia, 2 ml conc. NaOH, 2ml conc. HCl, 5ml
    bromine water, 5ml-10% NaOH, Sodium nitroprusside, distilled water
    IV. PROCEDURE

    Physical Test
    A. Test for specific Gravity
    Place a 90 ml of urine sample in a 100 ml graduated cylinder. Immerse
    hydrometer and determine the specific gravity of the urine sample.
    B. Color
    Note the color of the urine sample
    Chemical Test
    A. Reaction:
    Test the reason of urine with blue and red litmus paper.
    B. pH:
    Test pH of the urines sample using pH paper and pH meter.
    C. Detection of Albumin:
    Fill the test tube almost the top with clear urine. Heat (direct heating) the upper
    third to boiling point. The lower 2/3 point should not be hated. This portion will serve as
    your constant. If a cloud forms, aadd-4 drops of acetic acid. Heat once more and place in
    a rack. Observe after 5 minutes. Record your observation.
    NOTE: When the cloudiness remains after the addition of acid, it is due to albumin or nucleoproteins, if it
    disappears, it is due to phosphates.

    DO NOT agitate, stir or shake the urine during the two heating process.

    D. Detection of Glucose: Benedict’s test


    D.1. Place 3 ml of Benedict’s reagent in a clean test tube, then add 5 ml of
    urine. Mix thoroughly. Boil it in a water bath for 10-15 minutes. Allow the tube to cool
    and if glucose is present, red or yellow precipitate will appear. Record your observation.
    D.2. Mix equal amount of Fehling’s solution A and B in a test tube. Add
    enough water in the proportion of 1 is to 5 (1 part Fehling’s to 5 parts distilled water). To
    the solution, add 5 ml of urine sample. Bring to boil (in water bath), if glucose is present,
    red or yellow precipitate is formed. Record your observation.
    E. Test for Acetone: (Determine the degree of acidosis in diabetes mellitus).
    Lange’s test:
    Take about 10 ml of urine in a test tube, and add 10 drop of glacial acetic acid and
    2-4 drops of sodium nitroprusside reagent and run a little diluted ammonia upon
    its surface. If acetone is present, a reddish-purple ring will form within a few
    minutes at the junction of the two fluids. (DO NOT shake or stir or stir the
    mixtures). Record your observation. (watch a video)

    F. Detection of Urea:
    In a test tube, place 5 ml of urine then add 2 ml of conc. Sodium hydroxide and a
    few drops of bromine water. Note the production of nitrogen gas. Record your
    observation. (watch a video)

    G. Detection of Uric acid:


    In a test tube, place 5ml of urine in a beaker. Add 1 ml of conc. HCI, stir well and
    set side until the next laboratory period. Describe the crystal deposits on the side
    of the beaker. Record your observation.

    DATA
    PHYSICAL TEST CHEMICAL TEST

    NAME Vol.of Specific Color


    sample gravity
    Limus papaer

    pH meter
    pH paper

    Uric acid
    Albumin

    Acetone
    Glucose
    BLUE

    RED

    Urea
    IV. RESULTS AND OBSERVATION

    1. What is the significance in getting the physical characteristics of urine?


    __________________________________________________________________
    __________________________________________________________________

    2. What would the presence of albumin indicates?


    __________________________________________________________________
    __________________________________________________________________
    3. What would the presence of glucose indicates?
    __________________________________________________________________
    __________________________________________________________________
    4. What would the presence of acetone indicates?
    __________________________________________________________________
    __________________________________________________________________
    5. What would the presence of urea indicates?
    __________________________________________________________________
    __________________________________________________________________
    6. Why must a 24-hr period sample of urine be used for examination if a detailed
    composition is to be determined?
    __________________________________________________________________
    __________________________________________________________________
    For a simple routine qualitative analysis, why is an early sample of urine is
    used for the test, and not a sample collected after meal?
    ____________________________________________________________
    ____________________________________________________________
    7. What is meant by glycosuria?
    __________________________________________________________________
    __________________________________________________________________
    Albiminuria?
    __________________________________________________________________
    __________________________________________________________________
    8. What volume of urine does an adult excrete daily?
    __________________________________________________________________
    __________________________________________________________________
    9. What is the pH range of urine?
    __________________________________________________________________
    __________________________________________________________________
    10. What are the normal constituents of urine (male and female)?
    __________________________________________________________________
    __________________________________________________________________
    What are the abnormal constituents of urine?
    __________________________________________________________________
    __________________________________________________________________

    V. CONCLUSION

    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________
    __________________________________________________________________

    You might also like