Scribd Logo
Upload

Welcome to Scribd!

  • 26 views

    Cleaning and Disinfection of Microbiology Lab

    Uploaded by

    Naveen Kumar
    1. The document outlines the standard operating procedure for operating and calibrating a conductivity meter in the cell culture department of Laurus Bio Pvt. Ltd. 2. It details the responsibilities and procedures for cleaning, operating, and calibrating the conductivity meter, including preparing the meter, taking measurements, and recording results. 3. The calibration procedure involves immersing the probe in standard solutions and confirming the readings to ensure accurate conductivity measurements.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Cleaning and Disinfection of Microbiology Lab

    Uploaded by

    Naveen Kumar
    26 views5 pages
    1. The document outlines the standard operating procedure for operating and calibrating a conductivity meter in the cell culture department of Laurus Bio Pvt. Ltd. 2. It details the responsibilities and procedures for cleaning, operating, and calibrating the conductivity meter, including preparing the meter, taking measurements, and recording results. 3. The calibration procedure involves immersing the probe in standard solutions and confirming the readings to ensure accurate conductivity measurements.

    Original Description:

    Cleaning and Disinfection of Microbiology Lab

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    1. The document outlines the standard operating procedure for operating and calibrating a conductivity meter in the cell culture department of Laurus Bio Pvt. Ltd. 2. It details the responsibilities and procedures for cleaning, operating, and calibrating the conductivity meter, including preparing the meter, taking measurements, and recording results. 3. The calibration procedure involves immersing the probe in standard solutions and confirming the readings to ensure accurate conductivity measurements.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    26 views5 pages

    Cleaning and Disinfection of Microbiology Lab

    Uploaded by

    Naveen Kumar
    1. The document outlines the standard operating procedure for operating and calibrating a conductivity meter in the cell culture department of Laurus Bio Pvt. Ltd. 2. It details the responsibilities and procedures for cleaning, operating, and calibrating the conductivity meter, including preparing the meter, taking measurements, and recording results. 3. The calibration procedure involves immersing the probe in standard solutions and confirming the readings to ensure accurate conductivity measurements.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    You are on page 1of 5

    LAURUS BIO (P) LTD

    STANDARD OPERATING PROCEDURE


    Operation and Calibration of Conductivity
    Title:
    Meter
    SOP No.: Department Cell culture
    Effective date Review date Page No.
    Supersedes No. Nil Date of issue 1 of 3

    1.0 OBJECTIVE
    To lay down a procedure for receipt, issuance, cleaning and sanitization of QC
    Bioanalytical Laboratory by using appropriate disinfection methods.

    2.0 SCOPE
    This procedure is applicable for the classified area of QC Bioanalytical Laboratory
    Department at Laurus Bio Pvt. Ltd., Bengaluru.

    3.0 RESPONSIBILITY
    QC Bioanalytical Lab

    4.0 ACCOUNTABILITY
    Department Head.

    5.0 DEFINITIONS
    Disinfectant: A chemical or physical agent that destroys or removes vegetative forms
    of microorganisms.

    6.0 PROCEDURE
    6.1 Requirements
    6.2 Disinfectants and fumigants,
    6.3 10 ml micropipette
    6.4 Mops
    6.5 Buckets for disinfectants
    6.6 RO water
    6.7 Measuring cylinders
    6.8 Lint free wipes
    6.9 Autoclavable bags
    6.10 Autoclave, Weighing Balance and pH meter

    6.1 Procedure for Operation of Conductivity meter


    6.1.1 Ensure that the instrument is clean and free from dust.
    6.1.2 Ensure that the instrument is calibrated.
    6.1.3 Wash the conductivity meter sensor with clean water.
    PREPARED BY REVIEWED BY APPROVED

    Cell Culture Head of the Quality


    Depart Depa
    ment rtme
    nt

    Sign: Sign: Sign:


    Date: Date: Date:
    CCFQA001/F01-00
    LAURUS BIO (P) LTD
    STANDARD OPERATING PROCEDURE
    Operation and Calibration of Conductivity
    Title:
    Meter
    SOP No.: Department Cell culture
    Effective date Review date Page No.
    Supersedes No. Nil Date of issue 2 of 3

    6.1.4 Dry the conductivity meter probe with lint free wipes.
    6.1.5 Switch “ON” the conductivity meter by pressing the “ON/OFF” button.
    6.1.6 Dip the probe into the sample solution.
    6.1.7 The end of the sensor shall be completely immersed into the sample. Stir the probe
    gently to create a homogenous sample.
    6.1.8 Read the conductivity on the display in mS/cm or µS/cm.
    6.1.9 Allow the reading to stabilize. Record the details in Annexure.
    6.1.10 Remove the conductivity meter probe.
    6.1.11 Switch “OFF” the conductivity meter by pressing the “ON/OFF” button.
    6.1.12 Rinse the sensor with clean water and cover the sensor with the included cap after
    use.
    6.1.13 Clean the exterior surface of the Conductivity Meter with 70% IPA using lint free
    cloth.

    6.2 Procedure for Calibration of Conductivity meter


    6.2.1 PCS Testr 35 is capable of automatic and manual conductivity calibration.
    6.2.2 In automatic calibration mode, the instrument will automatically choose one of the
    three conductivity calibration standards depending on the ranges listed below.

    Conductivity Range Automatic Calibration Value


    0.0 – 200.0 µS 84 µS
    201 – 2000 µS 1413 µS
    2.01 – 20.00 µS 12.88 mS

    6.2.3 PCS Testr 35 is capable of single or multi-point conductivity calibration.


    6.2.4 Selection of muti-point calibration shall allow up to three of the following values,
    while single-point calibration shall allow only one (84 µS, 1413 µS or 12.88 mS) of
    the calibration standard.
    6.2.5 To calibrate the instrument, press ON/OFF to turn the Conductivity meter on and
    press MODE ENT to select conductivity mode.
    6.2.6 Rinse the sensor with clean water.
    6.2.7 Immerse the sensor into the calibration standard.
    6.2.8 The end of the sensor shall be completely immersed into the sample. Stir the probe

    PREPARED BY REVIEWED BY APPROVED

    Cell Culture Head of the Quality


    Depart Depa
    ment rtme
    nt

    Sign: Sign: Sign:


    Date: Date: Date:
    CCFQA001/F01-00
    LAURUS BIO (P) LTD
    STANDARD OPERATING PROCEDURE
    Operation and Calibration of Conductivity
    Title:
    Meter
    SOP No.: Department Cell culture
    Effective date Review date Page No.
    Supersedes No. Nil Date of issue 3 of 3

    gently to create a homogenous sample.


    6.2.9 Press CAL The primary display will show the un-calibrated value. The secondary
    display shall search for and lock on the closest automatic calibration value.
    6.2.10 Allow the primary display to stabilize, then press MODE ENT to confirm the
    calibration value.
    6.2.11 The primary value shall blink briefly before returning to measurement mode.
    6.2.12 Repeat steps 6.2.6 to 6.2.10 with additional calibration standards if required.
    6.2.13 Record the calibration details in Annexure.

    7.0 REFERENCES
    Not applicable

    8.0 ABBREVIATIONS

    Abbreviations Full description


    Pvt. Private
    Ltd. Limited
    PPE Personal Protective Equipment
    IPA Iso Propyl Alcohol
    % Percent

    9.0 ANNEXURES
    Not applicable

    PREPARED BY REVIEWED BY APPROVED

    Cell Culture Head of the Quality


    Depart Depa
    ment rtme
    nt

    Sign: Sign: Sign:


    Date: Date: Date:
    CCFQA001/F01-00
    LAURUS BIO (P) LTD
    STANDARD OPERATING PROCEDURE
    Operation and Calibration of Conductivity
    Title:
    Meter
    SOP No.: Department Cell culture
    Effective date Review date Page No.
    Supersedes No. Nil Date of issue 4 of 3

    PREPARED BY REVIEWED BY APPROVED

    Cell Culture Head of the Quality


    Depart Depa
    ment rtme
    nt

    Sign: Sign: Sign:


    Date: Date: Date:
    CCFQA001/F01-00
    LAURUS BIO (P) LTD
    STANDARD OPERATING PROCEDURE
    Operation and Calibration of Conductivity
    Title:
    Meter
    SOP No.: Department Cell culture
    Effective date Review date Page No.
    Supersedes No. Nil Date of issue 5 of 3

    PREPARED BY REVIEWED BY APPROVED

    Cell Culture Head of the Quality


    Depart Depa
    ment rtme
    nt

    Sign: Sign: Sign:


    Date: Date: Date:
    CCFQA001/F01-00

    You might also like