Biochemistry Review Guide
Biochemistry Review Guide
Biochemistry Review Guide
Reactions
What is an endergonic reaction? Exergonic?
Define anabolism. Define catabolism. What are exchange reactions?
Molecules
Know the difference between organic and inorganic compounds.
Know the important biological features of water. How is it polar? How does it
act as a solvent?
Know the properties of an acid, a base and a salt. What does pH of a solution
mean?
Organic molecules
What is a carbon skeleton?
What is a functional group?
What are macromolecules? How are they made?
Macromolecules
Carbohydrates
Chemical make-up, Different sizes, how they are made from monomers
Lipids
General characteristics, fats, phospholipids, steroids
Proteins
Functions, amino acid structure, stereoisomers, peptide bonds, levels of
protein structure, denaturation, conjugated proteins
Nucleic Acids
Nucleotide structure, nucleoside structure, DNA vs RNA structure and
function,
ATP
Structure
Function
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Protein Practice Questions
2. Draw an amino acid. Identify the N-C-C backbone and residue (R group).
5. Draw a chemical reaction where two amino acids are joined via a dehydration
synthesis. Identify the peptide bond. Is this endergonic or exergonic? Catabolic
or Anabolic? Synthesis or decomposition? Require Hydrolysis or Dehydration?
Primary
Secondary
Tertiary
Quaternary
8. Do all functional proteins have primary and secondary structure? Why or why
not?
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9. What are the two types of secondary structure.
10. How can you determine if a sequence of amino acids would be found
internally to the protein (Hint, look at #3 above) or externally depending on the
residues? (Think about hydrophobic v hydrophilic regions.)
1. What are the two types of nucleic acids full names and acronyms?
3. Draw a nucleotide. Label the phosphate, nitrogenous base, sugar, and number
the carbons in the sugar.
4. What are the 5 different nitrogenous bases and which nucleic acid can they be
found in? Which are purines and which are pyrimidines?
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5. What holds the nucleic acid monomers together to make the backbone?
6. What holds the two strands of DNA together? How many of this type of bond
the different bases together?
8. What molecular group is found at the 3’ end of a nucleic acid strand? What
molecular group is the 5’ end?
9. What does it mean for a nucleic acid to be antiparallel? (you can draw this
one)
10. What are the base pairing rules for DNA? For RNA?
11. Explain why a Purine must be matched to a Pyrimidine for the width of the
double-helix to be consistent. (You can draw this one also with an explanation.)