Proceeding Paper

Exploring the Antiradical Potential of Species from Lamiaceae

Family: Implications for Functional Food Development in the
Context of Neurodegenerative and Neuropsychiatric Diseases †
Ana Campinho 1 , Joana Alves 1 , Rosário Martins 2,3 , Mónica Vieira 4,5 , Clara Grosso 1, *
and Cristina Delerue-Matos 1

1 REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr.
António Bernardino de Almeida, 431, 4249-015 Porto, Portugal; 1210048@isep.ipp.pt (A.C.);
10200666@ess.ipp.pt (J.A.); cmm@isep.ipp.pt (C.D.-M.)
2 CIIMAR/CIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto,
Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal;
mrm@ess.ipp.pt
3 Health and Environment Research Centre, School of Health, Polytechnic Institute of Porto, Rua Dr. António
Bernardino de Almeida, 400, 4200-072 Porto, Portugal
4 Ciências Químicas e das Biomoléculas, Escola Superior de Saúde, Instituto Politécnico do Porto, Rua Dr.
António Bernardino de Almeida 400, 4200-072 Porto, Portugal; mav@ess.ipp.pt
5 TBIO—Centro de Investigação em Saúde Translacional e Biotecnologia Médica, Escola Superior de Saúde,
Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 400, 4200-072 Porto, Portugal
* Correspondence: claragrosso@graq.isep.ipp.pt
† Presented at the 4th International Electronic Conference on Foods, 15–30 October 2023; Available online:
https://foods2023.sciforum.net/.

Abstract: Neurodegenerative and neuropsychiatric diseases have become highly significant in West-
ern societies. Unfortunately, these diseases currently lack a cure, and existing treatments merely
manage the symptoms. Thus, it is imperative to explore new alternatives for either preventing these
disorders or treating them effectively. One promising avenue for prevention lies in the development
of neuroprotective and antioxidant functional foods. To this end, a study focused on ten species
Citation: Campinho, A.; Alves, J.; from the Lamiaceae family, which have attracted attention due to their well-known antioxidant,
Martins, R.; Vieira, M.; Grosso, C.; anti-inflammatory, anti-obesity, and anti-cancer properties, among others. The interest in their phar-
Delerue-Matos, C. Exploring the macological applications has grown significantly in recent years. In order to uncover the biological
Antiradical Potential of Species from potential of these species, the study involved performing decoctions and evaluating both the total
Lamiaceae Family: Implications for phenolic content (TPC) and antiradical activity. The results revealed that TPC values ranged from
Functional Food Development in the 59.97 ± 6.18 (Ocimum basilicum L. var minimum) to 374.0 ± 16.9 (Salvia officinalis L.) mg gallic acid
Context of Neurodegenerative and
equivalents (GAE)/g of dry extract (dw). Additionally, the IC50 values for DPPH• and ABTS•+
Neuropsychiatric Diseases. Biol. Life
scavenging activities varied between 21.55 ± 1.18 (Origanum vulgare L.) and 132.0 ± 15.3 µg/mL
Sci. Forum 2023, 26, 33. https://
(O. basilicum var minimum), and from 14.79 ± 0.50 (O. vulgare) to 44.65 ± 2.34 µg/mL (O. basilicum),
doi.org/10.3390/Foods2023-15493
respectively. The observed strong antiradical activity holds great promise for the future develop-
Academic Editor: Manuel ment of functional foods aimed at combating the oxidative stress implicated in these diseases and
Viuda-Martos promoting overall brain health. By harnessing the potential of these species from Lamiaceae family,
Published: 30 October 2023 we may pave the way for innovative approaches to tackle neurodegenerative and neuropsychiatric
conditions.

Keywords: Lamiaceae family; brain disorders; functional foods; oxidative stress

Copyright: © 2023 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
1. Introduction
conditions of the Creative Commons
Attribution (CC BY) license (https://
Oxidative stress is characterized by the imbalance between reactive oxygen species
creativecommons.org/licenses/by/ (ROS) and antioxidant defense mechanisms, and is a major contributor to the pathogenesis
4.0/).

Biol. Life Sci. Forum 2023, 26, 33. https://doi.org/10.3390/Foods2023-15493 https://www.mdpi.com/journal/blsf

Biol. Life Sci. Forum 2023, 26, 33 2 of 5

of several disorders [1], including cardiovascular diseases [2], diabetes [3], neurodegenera-
tive and neuropsychiatric diseases [4,5], and cancer [6]. Consumption of nutraceuticals and
functional foods rich in antioxidants is a suitable strategy to delay the progression of these
chronic disorders, since dietary supplementation will boost the antioxidant status of the
body, enabling the reduction in the production of oxidative stress biomarkers [7]. Antioxi-
dants are, therefore, highly demanded for nutraceutical and functional food products by
product development companies. Compared with synthetic antioxidants, such as butylated
hydroxytoluene (BHT) and butylated hydroxyanisole (BHA), natural antioxidants offer
great advantages since they are considered safer and are also economically and easily
available. They can be extracted from different natural matrices, such as edible vegetables,
aromatic plants, fruits, seeds, agri-food by-products, algae, etc. [8,9].
The Lamiaceae family, commonly known as the mint family, is composed of 236 genera
and around 6900 to 7200 species, with a worldwide distribution [10]. Several species are
known for their pharmacological potential, that results from a wide range of secondary
metabolites produced, mainly flavonoids, phenolic acids, and terpenoids [10]. Amongst
the bioactive properties reported, their antioxidant, anti-inflammatory, antimicrobial, and
neuroprotective properties are the most studied ones [10,11].
The aim of this study was to valorize 10 Lamiaceae species belonging to the Lavandula,
Mentha, Ocimum, Origanum, Rosmarinus, Salvia and Thymus genera, by assessing their
antioxidant activity and possible utilization in the design of functional food products for
neurodegeneration and neuropsychiatric prevention.

2. Material and Methods

2.1. Plant Species
Ten different species from the Lamiaceae family were purchased from an herbal store
(Ervanário Portuense, Portugal, https://www.ervanarioportuense.pt, accessed on 01 March
2023); namely, Lavandula angustifolia Miller (Lot 10.ALF.109.22.02), Mentha piperita L. (Lot
07.HOR.51102.21.11S), Mentha pulegium L. (Lot 02.POE.117J.22.2S), Ocimum basilicum L. (Lot
10.BAS.109.21.03), Ocimum basilicum var. minimum L. (Lot 10.MNJ.660.14.1C), Origanum
majorana L. (Lot 1402TR), Origanum vulgaris L. (Lot 11.ORE.1078.20.01), Rosmarinus officinalis
L. (Lot 11.ALE.117.22.01), Salvia officinalis L. (Lot 1922ALS), and Thymus vulgaris L. (Lot
12.TOM.117.22.01). All plant materials were powdered to a mean particle size of < 1000 µm
and stored at room temperature before use.

2.2. Extraction Procedure

Extracts were prepared by boiling 0.5 g of each powdered plant material in 125 mL of
water for 10 min. After this step, the extracts were filtered and lyophilized.

2.3. Determination of Total Phenolic Compounds (TPC)

A spectrophotometric assay based on the Folin–Ciocalteu reagent [12] was employed
to determine the TPC values of each extract, and calibration curves were performed using
gallic acid. The formation of the blue complex was monitored at 760 nm in a microplate
reader (Synergy HT, Biotek Instruments, Winooski, VT, USA). Results were expressed as
mg gallic acid equivalents (GAE)/g of extract dried weight (dw). Three independent assays
were performed.

2.4. Antiradical Acitvity

The antiradical capacity was assessed against two radicals, namely, DPPH• and
ABTS•+ , according to well-established procedures [12]. Absorbances were monitored
in a microplate reader (Synergy HT, Biotek Instruments, Winooski, VT, USA) at 517 and
734 nm, respectively. Each sample was tested in triplicate and the results are expressed as
IC50 values.
Biol. Life Sci. Forum 2023, 26, 33 3 of 5

2.5. Statistical Analysis

For both TPC values and antiradical activities, samples were compared using a one-
way analysis of variance (ANOVA) followed by the Tukey’s test. p-values of less than
0.05 were considered statistically significant. A Pearson correlation between TPC and
bioactivities was also performed. All statistical analyses were carried out with GraphPad
Prism, version 8.0.1.

3. Results and Discussion

Table 1 displays the TPC values determined for the ten decoctions tested, ranging
from 59.97 to 374.0 mg GAE/g extract dw. Among all samples, S. officinalis stood out for
its highest content of phenolic compounds (374.0 mg GAE/g extract dw), followed by R.
officinalis (195.1 mg GAE/g extract dw), the Mentha species (188.9 and 140.4 mg GAE/g
extract dw) and the Origanum species (156.6 and 118.6 mg GAE/g extract dw). Brezoiu
et al. [13] reported a TPC value for the S. officinalis hydroethanolic (ethanol/water = 4/1
v/v) extract that was lower than the one shown in Table 1 (181.11 mg GAE/g extract dw),
which may be related to the different solvent used for the extraction procedure. Indeed,
Schnitzler et al. [14] compared different extraction solvents when extracting phenolic
compounds from S. officinalis, and concluded that water achieved better results than all
the tested ethanol–water mixtures. Concerning the Mentha species, the TPC values of
17.00 mg GAE/g dw (for M. pulegium) and 31.40 mg GAE/g dw (for M. piperita) were
obtained for 80% aqueous methanolic extracts [15], while, for an aqueous extract of M.
piperita, the value recorded was 230.8 mg GAE/g [16]. Yan et al. [17] determined the
TPC values for 42 O. vulgare samples, belonging to five subspecies from an oregano plant
collection of the German National Genebank, and obtained values between 79.5 mg GAE/g
dw and 147.3 mg GAE/g dw for their 80% (v/v) hydromethanolic ultrasound-assisted
extracts. The results obtained for O. majorana are better than those previously reported
for an aqueous extract (9.2 mg GAE/g) [18], while the ones found for R. officinalis are in
agreement with those described before [19].

Table 1. Total phenolic content (TPC), and DPPH• and ABTS•+ scavenging activities of the ten
decoctions.

DPPH• Scavenging
TPC ABTS•+ Scavenging Activity
Species Activity
(mg GAE/g dw) (IC50 , µg/mL)
(IC50 , µg/mL)
Lavandula angustifolia Miller 94.97 ± 11.82 e 42.66 ± 0.98 c,d 36.36 ± 1.71 b
Mentha piperita L. 188.9 ± 6.5 b 34.52 ± 3.76 c,d 28.17 ± 2.52 c
Mentha pulegium L. 140.4 ± 4.1 c,d 43.31 ± 1.90 c 25.15 ± 2.74 c
Ocimum basilicum L. 68.32 ± 8.92 e,f 40.41 ± 1.57 c,d 44.65 ± 2.34 a
Ocimum basilicum var
59.97 ± 6.18 f 132.0 ± 15.3 a 37.45 ± 1.12 b
minimum L.
Origanum majorana L. 118.6 ± 14.4 d,e 54.71 ± 17.13 b 24.83 ± 0.80 c
Origanum vulgare L. 156.6 ± 9.2 c 21.55 ± 1.18 d 14.79 ± 0.50 d
Rosmarinus officinalis L. 195.1 ± 18.3 b 25.78 ± 1.13 c,d 19.06 ± 0.57 d
Salvia officinalis L. 374.0 ± 16.9 a 29.64 ± 1.71 c,d 28.04 ± 0.39 c
Thymus vulgaris L. 70.96 ± 4.24 e,f 43.77 ± 0.36 c 28.50 ± 0.91 c
Results are expressed as mean ± standard deviation of three assays (n = 3). In each column, different superscript
letters mean statistically significant differences, at p < 0.05.

Concerning DPPH• scavenging activity, all plant extracts were active, displaying
IC50 values in the range of 21.55 µg/mL (O. vulgare) and 132.0 µg/mL (O. basilicum var.
minimum), with the order of potency being as follows: O. vulgare ≈ R. officinalis ≈ S.
officinalis ≈ M. piperita ≈ O. basilicum ≈ L. angustifolia ≈ M. pulegium ≈ T. vulgaris > O.
majorana > O. basilicum var minimum. Except for the study published by Dorman et al. [16],
in which the reported IC50 values of the aqueous extracts against DPPH• were higher (e.g.,
Biol. Life Sci. Forum 2023, 26, 33 4 of 5

335.0 µg/mL for O. vulgare, 236.5 µg/mL for R. officinalis, 265.8 µg/mL for S. officinalis,
382.4 µg/mL for T. vulgaris, and c.a. 150 µg/mL for M. piperita), all the values reported
by other authors for aqueous and hydroethanolic extracts are in the same range as those
presented in Table 1 [15,18,20–24].
The strongest ABTS•+ scavenging activity was observed for O. vulgare and R. officinalis,
followed by O. majorana ≈ M. pulegium ≈ S. officinalis ≈ M. piperita ≈ T. vulgaris > L.
angustifolia > O. basilicum var minimum ≈ O. basilicum (Table 1), and the obtained IC50
values are in the same range of those determined by other authors. Mapeka et al. [25] tested
different extracts of Lamiaceae species, and the IC50 values were as follows: O. majorana
(IC50 = 5.79 µg/mL), R. officinalis (IC50 = 10.56 µg/mL), S. officinalis (IC50 = 17.18 µg/mL),
M. piperita (IC50 = 19.96 µg/mL), T. vulgaris (IC50 = 27.48 µg/mL), and O. basilicum (IC50 =
53.54 µg/mL).
The correlation analysis between TPC and the antiradical activity data showed that
there is not a strong correlation between chemical composition and bioactivities
(r = −0.454 between TPC and DPPH• scavenging activity, r = 0.451 between TPC and
ABTS•+ scavenging activity, and r = −0.400 between both antiradical activities), meaning
that other classes of compounds may also contribute to the overall activity.

4. Conclusions
In this study, 10 decoctions prepared from Lamiaceae species were evaluated for
their potential antiradical activity. All extracts displayed strong activity, holding great
promise for the future development of functional foods designed to combat the oxidative
stress implicated in chronic disorders, such as neurodegenerative and/or neuropsychiatric
diseases.

Author Contributions: Conceptualization, R.M., M.V., C.G., and C.D.-M.; methodology, R.M., M.V.,
C.G., and C.D.-M.; validation, R.M., M.V., C.G., and C.D.-M.; formal analysis, R.M., M.V., C.G.,
and C.D.-M.; investigation, A.C. and J.A.; resources, R.M. and C.D.-M.; writing—original draft
preparation, A.C. and J.A.; writing—review and editing, A.C., J.A., R.M., M.V., C.G., and C.D.-M.;
visualization, A.C., J.A., R.M., M.V., C.G., and C.D.-M.; supervision, R.M., M.V., C.G., and C.D.-M.;
funding acquisition, R.M. and C.D.-M. All authors have read and agreed to the published version of
the manuscript.
Funding: This work was supported by projects REQUIMTE/LAQV—UIDB/50006/2020, UIDP/
50006/2020, and LA/P/0008/2020 financed by FCT/Ministério da Ciência, Tecnologia e Ensino
Superior (MCTES), through national funds.
Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.
Data Availability Statement: The data presented in this study are available on request from the
corresponding author.
Conflicts of Interest: The authors declare no conflict of interest.

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